JP2007086026A - Kit for immuno-chromatography, and testing vessel - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a kit for immuno-chromatography capable of detecting the material to be detected, without using a storage case or a test tube rack. <P>SOLUTION: The kit for immuno-chromatography comprises a test instrument for immuno-chromatography for detecting an object matter to be inspected in the specimen; and a testing vessel capable of storing the specimen. The test instrument comprises a specimen addition part that is to be dipped in the specimen stored in the testing vessel; a label maintaining part for holding the label material which generates the antigen-antibody reaction with the detection object in the specimen; and the determination part, where the matter is fixed that generates antigen-antibody reaction with the material to be detected. The testing vessel, equipped with a hook for holding the vessel by hanging, in a state of being hung by the hook, the material to be detected; can be detected. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an immunochromatography kit and a test container used for immunochromatography.

  There is a method of using immunochromatography as a method for easily examining various diseases using a body fluid such as blood, serum, pharyngeal wiping fluid, and urine as a specimen.

  Immunochromatography can be performed using an immunochromatography kit comprising an immunochromatographic test tool for detecting a substance to be detected in a sample and a test container that can contain the sample. Such an immunochromatography kit is often used in the medical field.

  In conventional immunochromatography kits, a sample is usually injected into a test container, and the test tool is inserted into the test container. Then, the test container is placed in a test tube stand or a storage case placed on a desk. It is used by the method of arrange | positioning and leaving to stand for about 20 minutes.

  For example, in the winter season when colds and flu are prevalent, a large number of patients visit clinics and hospitals for medical examinations and treatments, and medical workers conduct examinations and examinations without the time to rest. . When conducting a disease test using the above-described conventional test container, a test container containing a sample collected from a patient and an immunochromatographic test device is mounted on a storage case or a test tube stand and a flat surface such as a desk. It must be placed on the site for a certain period of time. In the above-mentioned busy medical field, multiple inspections and operations are often performed simultaneously on a single desk. Therefore, a storage case or test tube stand with a test container attached when performing other operations. May be defeated. Moreover, since a storage case and a test tube stand must be used, a space for mounting them must be secured.

The present invention has been made in view of such circumstances, and provides an immunochromatography kit capable of detecting a substance to be detected without using a storage case or a test tube stand.

Means for Solving the Problems and Effects of the Invention

  The immunochromatography kit of the present invention comprises an immunochromatography test tool for detecting a substance to be detected in a sample and a test container that can contain the sample, and the test tool is housed in the test container. A sample addition part immersed in the sample, a label holding part for holding a labeling substance that causes an antigen-antibody reaction with a substance to be detected in the sample, and an immobilization substance that causes an antigen-antibody reaction with the target substance are fixed. The test container includes a hook part for hooking and holding the test container, and the detection target substance can be detected in a state where the test container is held by the hook part. is there.

  The immunochromatography kit of the present invention is provided with a hook portion in a test container for containing a sample, and the hook portion enables detection of a substance to be detected while the test container is held on a desired object. It is. The desired object is, for example, a healthcare worker's pocket (chest pocket, waist pocket, etc.), a chart binder, or the like.

  By performing the detection in a state where the test container is held on a desired object, the detection can be performed without using a test tube stand or a storage case. Further, by holding the hook portion in the pocket of a medical staff, the test container can be easily carried and can move around to various places while performing detection.

  It is preferable that the hook part provided in the test container of the present invention includes a base part connected to the test container and a hook body extending from the base part toward the bottom of the test container.

  The hook body may be configured such that the distance between the hook body and the test container is gradually increased as the distance from the base portion increases.

  The hook portion may further include a grip portion that extends in a direction opposite to the hook body, and the test container may further include a protruding portion that protrudes in the same direction as the grip portion.

  The hook body may have a non-slip portion on the surface facing the test container.

  The hook portion further includes a protrusion extending in the opposite direction of the hook body, and the shape of the opening edge of the test container is such that the hook body and the test container are arranged at a predetermined angle with the protrusion. It may be configured to abut and hold the hook body and the test container at a predetermined angle.

  The protruding portion may have a meshing portion that meshes with the opening edge.

  The protrusion may be configured to close the opening of the test container when the opening edge and the meshing portion are in contact with each other.

  The front end of the hook body may be configured to be wider than the bottom of the test container.

  The hook body may have a convex portion on the surface facing the test container.

  The test container may have a convex portion on the surface facing the hook body.

  The tip of the hook body may be substantially the same height as the bottom of the test container.

  A hook part is connected to the test container, a hook body extending from the base part toward the bottom of the test container, and a spacer that is connected to the test container and that faces the hook body. It is preferable that the surface of the spacer portion facing the hook body is inclined with respect to the major axis direction of the test container.

  The surface of the spacer portion facing the hook body and the surface of the hook body facing the spacer portion may be substantially parallel.

  The hook portion may be disposed near the center of the long axis direction of the test container.

  The hook part may have a magnet part.

  The hook part may have an opening.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. The drawings are used for convenience of explanation, and the scope of the present invention is not limited to the embodiments shown in the drawings.

  FIG. 1 shows an immunochromatography kit according to an embodiment of the present invention. This kit includes an immunochromatographic test device 1 for detecting a substance to be detected in a sample, and a test container 3 that can contain the sample. 2 (a), 2 (b), 3 (a), and 3 (b) are a side view and a front view, respectively, of the test device 1 and the test container 3 of FIG. In addition, the figure of the test container 3 displays what was seen from the upper part for convenience of illustration.

  As shown in FIGS. 2 (a) and 2 (b), the test device 1 includes a sample addition member 7 made of a rayon nonwoven fabric and a glass fiber on a base material 5 made of a plastic plate having an adhesive layer on the surface. A label holding member 9 made of a nonwoven fabric, a chromatographic membrane carrier 11 made of a nitrocellulose porous body, and an absorption member 13 made of a cellulose nonwoven fabric are provided. The sample addition member 7 functions as a sample addition unit that is immersed in the sample accommodated in the test container 3. The label holding member 9 is disposed in contact with the sample addition member 7 and functions as a label holding unit that holds a labeling substance that causes an antigen-antibody reaction with a substance to be detected in the sample. The chromatographic membrane carrier 11 is disposed in contact with the label holding member 9 and has a determination unit to which an immobilization substance that causes an antigen-antibody reaction with a substance to be detected is immobilized. The absorption member 13 is disposed so as to contact the chromatographic membrane carrier 11.

  In the chromatographic membrane carrier 11, a linear first determination unit 11A, second determination unit 11B, and control unit 11C are formed in order from the upstream side. The label holding member 9 holds a first labeling substance, a second labeling substance, and a control labeling substance. In the first determination unit 11A, the second determination unit 11B, and the control unit 11C, anti-influenza A antibody and anti-influenza B antibody (hereinafter referred to as “anti-FluA antibody” and “anti-FluB antibody”, respectively) are used as immobilization substances. And biotin is immobilized. In addition, displays “A”, “B”, and “!” Indicating the types are printed at positions corresponding to the first determination unit 11A, the second determination unit 11B, and the control unit 11C, respectively. The first labeling substance and the second labeling substance are respectively an anti-FluA antibody and an anti-FluB antibody labeled with blue latex particles, and the control labeling substance is avidin labeled with red latex particles. The anti-FluA antibody and the anti-FluB antibody are respectively referred to as an influenza A virus that is a first detected substance and an influenza B virus that is a second detected substance (hereinafter referred to as “FluA virus” and “FluB virus”, respectively). ) And antigen-antibody reaction.

  Taking the FluA virus as an example, if the FluA virus is contained in the sample, the labeled anti-FluA antibody in the label holding member 9 recognizes a predetermined site of the FluA virus and binds by antigen-antibody reaction. To form a complex. Next, the anti-FluA antibody on the chromatographic membrane carrier 11 recognizes another site of the FluA virus and captures the complex. When the complex is captured, a blue line appears in the first determination unit 11A, and the FluA virus is detected visually.

  Avidin is not captured by the anti-FluA antibody and anti-FluB antibody on the chromatographic membrane carrier 11, but specifically binds to biotin, and is thus captured by biotin immobilized on the control portion 11C. When avidin is captured, a red line appears in the control part 11C, and it is visually observed that avidin has reached the control part 11C. Since the control unit 11C is provided downstream of the first determination unit 11A and the second determination unit 11B, it is confirmed that the sample has passed through the first determination unit 11A and the second determination unit 11B by checking the red line. It is confirmed.

  Hereinafter, the test container 3 will be described with reference to FIGS. The test container 3 has a bottomed cylindrical shape having an opening at the top, and is used by inserting one end of the test device 1 from the opening of the test container 3. The test container 3 includes a hook portion 15. By using the hook part 15, the detection target substance can be detected by performing an immunochromatography in a state where the test container 3 is held on a desired object. The hook portion 15 includes a base portion 17 connected to the test container 3 and a hook body 19 extending from the base portion 17 toward the bottom of the test container 3. The base 17 is disposed near the opening of the test container 3. The hook body 19 has a convex portion 21 on the surface facing the test container 3. The convex portion 21 is provided at the tip of the hook body 19. The hook main body 19 is wider toward the tip. The hook portion 15 may be integrally formed with the test container 3 or may be separately manufactured and attached to the test container 3. The test container 3 and the hook part 15 can be formed of resin, glass, metal or the like. The base portion 17 may be disposed at a position other than the vicinity of the opening of the test container 3, and the convex portion 21 may be disposed at a position other than the tip of the hook body 19. Further, the hook main body 19 may have a constant width toward the tip, or may have a narrow width.

Next, the usage method of the kit of this embodiment is demonstrated using FIG.
First, a predetermined amount of a sample 23 prepared by diluting a specimen such as a nasal cavity aspirate of a patient with a developing solvent is injected into the test container 3. Next, the test device 1 is inserted into the test container 3. Thereafter, the test container 3 is attached to the desired object 25 using the hook portion 15. Here, the breast pocket of a medical worker is assumed as an object.

  When left in this state for about 10 to 20 minutes, the sample 23 sequentially moves the sample addition member 7, the label holding member 9, the chromatographic membrane carrier 11, and the absorption member 13 by capillary action. When the sample 23 passes through the label holding member 9, the labeling substances (first, second and control labeling substances) held on the label holding member 9 are eluted in the developing solvent. If the sample contains FluA virus or FluB virus, a blue line 25a appears in the first determination unit 11A or the second determination unit 11B due to the above-described action. Regardless of the presence or absence of virus, a red line 25b appears in the control part 11C (FIG. 4 shows the case where the FluA virus is contained in the sample).

  When the kit of this embodiment is used, the medical staff can move around in various places while wearing the test container 3 in which the test device 1 is inserted in the breast pocket during the above-mentioned leaving period, and it takes 10 to 20 minutes. After a certain amount of time has passed, the test results can be confirmed at a favorite place. Therefore, the kit according to the present embodiment is very easy to use for busy health workers.

  So far, the specific embodiment has been described as an example. However, the present invention is not limited to this embodiment, and various modifications are possible.

  The substance to be detected is not particularly limited as long as it causes an antigen-antibody reaction, and examples thereof include cells such as bacteria, protists and fungi, viruses, proteins, polysaccharides and the like. For example, in addition to the above influenza virus, parainfluenza virus, RS virus, Mycoplasma pneumoniae, rotavirus, calcivirus, coronavirus, adenovirus, enterovirus, herpes virus, human immunodeficiency virus, hepatitis virus, pathogenesis of severe acute respiratory syndrome Viruses, Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus spyogenes, malaria parasite, other pathogens of various diseases such as digestive system diseases, central nervous system diseases, hemorrhagic fever, metabolites, carcinoembryonic antigens And tumor markers such as Shirahura and Shihora, and hormones.

  The base material 5 is for appropriately arranging the above-described members such as the sample addition member 7 and the label holding member 9, and various materials such as paper and glass can be used in addition to plastic. The sample addition member 7 can be formed of various materials such as glass fiber or cellulose fiber in addition to rayon. The label holding member 9 can be formed of various materials such as cellulose fiber in addition to glass fiber. In addition to nitrocellulose, the chromatographic membrane carrier 11 is nylon (for example, modified nylon having an amino group which may have a carboxyl group or an alkyl group as a substituent), polyvinylidene difluoride (PVDF), cellulose It can be formed of various materials such as acetate. The absorbing member 13 can be formed of various materials such as glass fiber in addition to cellulose. As the sample addition member 7, the label holding member 9, the chromatographic membrane carrier 11, and the absorption member 13, materials having various structures capable of developing the sample by capillary action can be used in addition to the nonwoven fabric or the porous material.

  The chromatographic membrane carrier 11 may include only one determination unit or two or more according to the type of the substance to be detected. Further, the chromatographic membrane carrier 11 may not include a control part. Moreover, the determination part / control part may not be formed in a line shape, and may be formed in, for example, a circular shape or a rectangular shape. The label holding member 9 may hold only one type of labeling substance, or may hold two or more types. Further, the label holding member 9 may not hold the control labeling substance. The labeling substance may be labeled with latex particles other than blue and red, metal particles such as gold, and dye molecules. When there are two or more kinds of labeling substances, each labeling substance may be labeled with a different color or the same color. Furthermore, the labeling substance and the control labeling substance may be labeled in different colors or in the same color.

  In the said embodiment, although the classification display of 11 A of 1st determination parts, the 2nd determination part 11B, and the comparison part 11C was printed on the membrane carrier 11 for chromatography, these classification displays may be attached | subjected by methods other than printing. However, it may not be attached. Further, when the test tool 1 is inserted into the test container 3, the above type display is attached to the position on the test container 3 corresponding to the positions of the first determination unit 11 </ b> A, the second determination unit 11 </ b> B, and the control unit 11 </ b> C. Good. The type display may be other than “A”, “B”, and “!”.

  Various antibodies or antigens can be used as the immobilization substance and the labeling substance. That is, when the substance to be detected is an antigen, the immobilization substance and the labeling substance use an antibody that causes an antigen-antibody reaction with this antigen, and when the substance to be detected is an antibody, the immobilization substance and the labeling substance are: An antibody that causes an antigen-antibody reaction with an antigen that causes an antigen-antibody reaction with this antibody or an antibody that is a substance to be detected can be used.

  The immobilization substance in the control part may be avidin, and the control labeling substance may be biotin. Further, the immobilization substance and the control labeling substance in the control part may be other than the combination of biotin and avidin. For example, a combination that binds by an antigen-antibody reaction may be used. For example, an antigen is used as the control labeling substance, and an antibody that causes an antigen-antibody reaction with the antigen is used as the immobilization substance in the control part. The reverse is also possible. As the control labeling substance, a substance that does not undergo antigen-antibody reaction with the substance to be detected or the immobilization substance of the determination unit is used.

  Hereinafter, various embodiments of the test container 3 will be shown.

(1) Test container having a hook portion with magnets FIGS. 5A and 5B are a side view and a front view of a test container 3 having a hook portion with a magnet, respectively.

  The test container 3 has a hook portion 15 having a magnet portion 29. The magnet portion 29 is formed near the center of the hook body 19. The magnet portion 29 can be formed by attaching a magnet to the hook body 19. In another embodiment, the hook portion 15 is made of a magnetic material, and the entire hook portion 15 is used as the magnet portion 29.

  FIG. 6 shows a use state of the kit having the test container 3. According to FIG. 6, the test container 3 is attached to the iron wall 31 using the magnet portion 29. According to the present embodiment, the test container 3 can be held on the object by the magnet even with respect to the object on which the hook portion cannot be hooked, which is convenient.

(2) Test container having hook portion with opening FIGS. 7A and 7B are a side view and a front view of a test container 3 having a hook portion with an opening, respectively.

  The test container 3 has a hook portion 15 having an opening 33. The opening 33 is formed near the center of the hook body 19. The opening 33 can have any shape such as an ellipse, a circle, or a rectangle.

  FIG. 8 shows a use state of the kit having the test container 3. According to FIG. 8, the test container 3 is attached to a pin 37 piercing the wall 35 using the opening 33. According to the present embodiment, the test container 3 can be held on the object by the opening 33 even for an object to which the hook portion cannot be hooked, which is convenient.

(3) Test container having a stepped hook portion FIGS. 9A and 9B are a side view and a front view of a test container 3 having a stepped hook portion, respectively.
The hook main body 19 of the hook portion 15 of the test container 3 is configured such that the distance between the hook main body 19 and the test container 3 is gradually increased as the distance from the base portion 17 increases. Therefore, the surface of the hook body 19 that faces the test container 3 is stepped. The stage may be two stages or three or more stages. The hook body 19 has a convex portion 21 facing the test container 3 at each stage. In the present embodiment, the back surface of the hook body 19 is planar, but the thickness of the hook body 19 may be uniform and the back surface of the hook body 19 may be stepped.

  FIGS. 10A and 10B show the usage state of the kit having the test container 3. 10A shows a state when the test container 3 is attached to the relatively thin object 39, and FIG. 10B shows a state when the test container 3 is attached to the relatively thick object 41. Show. In the case of FIG. 10A, the object 39 is held at a portion where the distance between the hook body 19 and the test container 3 is narrow, and in the case of FIG. 10B, the object 41 is held at a portion where the distance is wide. is doing. Thus, according to the present embodiment, the test container 3 can be held on an object having a wide range of thicknesses.

(4) Test container having a hook part with a grip part FIGS. 11A and 11B are a side view and a front view of a test container 3 having a hook part with a grip part, respectively.
The hook portion 15 of the test container 3 further includes a grip portion 43 extending in the opposite direction of the hook body 19, and the test container 3 further includes a protruding portion 45 protruding in the same direction as the grip portion 43. . By grasping the grip portion 43 and the protruding portion 45 and moving the grip portion 43 in the direction of the arrow, the distance between the hook main body 19 and the test container 3 can be widened, corresponding to objects of various thicknesses. be able to. The surface of the grip portion 43 facing the test container 3 is preferably provided with a non-slip portion (not shown). The anti-slip portion can be formed by sticking an anti-slip member such as a rubber sheet on this surface or by forming a jagged surface on the hook body 19. The base portion 17 of the hook portion 15 is made thin so that the angle between the hook body 19 and the test container 3 can be changed relatively freely. A spring member that applies a force in a direction in which the hook body 19 approaches the test container 3 may be attached to the base portion 17 and the like. The protrusion 45 can be integrally formed with the test container 3. The protrusion 45 is preferably formed thicker than the test container 3 in order to have a certain degree of rigidity.

  FIG. 12 shows a usage state of the kit having the test container 3. According to FIG. 12, the test container 3 is attached to a relatively thick object 46 using the hook portion 15. Thus, according to the present embodiment, the test container 3 can be held on the objects having various thicknesses.

(5) Test container with long hook body of hook part FIGS. 13A and 13B are a side view and a front view of the test container 3 with long hook body of the hook part, respectively.

  The hook body 19 of the hook portion 15 of the test container 3 has a tip 19 a that is substantially the same height as the bottom 3 a of the test container 3. “Substantially the same” means that the difference in height between the tip 19 a and the bottom 3 a is generally within 10% of the total length of the test container 3. However, it is preferable that the tip 19a is on the base 17 side with respect to the bottom 3a so that the bottom 3a contacts the mounting surface before the tip 19a when placed on a desk or the like.

  FIG. 14 shows a usage state of the kit having the test container 3. According to FIG. 14, the test container 3 is attached to a short plate-like object 49 protruding vertically from the desk 47 using the hook portion 15. When the test container 3 shown in FIG. 3 or the like is used, the bottom of the test container 3 comes into contact with the desk 47 before the hook portion 15 and the object 49 come into contact with each other, so that the test container 3 is attached to the object 49. Is difficult. According to the present embodiment, since the hook body 19 is sufficiently long, the test container 3 can be easily attached to the object 49.

(6) Test container having a convex portion on the surface facing the hook main body FIGS. 15A and 15B are a side view and a front view of the test container 3 having the convex portion on the surface facing the hook main body, respectively. is there.

  The hook main body 19 of the test container 3 has a convex portion 21 on the surface facing the test container 3, and the test container 3 has a convex portion 51 on the surface facing the hook main body 19. By sandwiching the target object between the convex part 21 and the convex part 51, the test container 3 is unlikely to be detached from the target object. 15 (a) and 15 (b) show an example using a long hook portion as shown in FIGS. 13 and 14, but also when a short hook portion as shown in FIGS. 1 to 12 is used. Similar effects can be obtained. Further, the lower surface 51 a of the convex portion 51 is preferably on the same plane as the bottom 3 a of the test container 3. In this case, the test container 3 can be further stabilized when the test container 3 is attached to the object.

  FIG. 16 shows the usage state of the kit having the test container 3. According to FIG. 16, the short plate-like object 49 projecting vertically from the desk 47 is held in a state of being bent between the convex portion 21 and the convex portion 51. For this reason, according to the present embodiment, the object 49 is unlikely to be detached from the test container 3.

(7) Test container having hook portion with protrusions FIGS. 17A and 17B are a side view and a front view of the test container 3 having hook portions with a protrusion, respectively.

  The hook portion 15 of the test container 3 further includes a protruding portion 53 that extends in the direction opposite to the hook body 19. Further, the opening edge 3b of the test container 3 abuts on the protrusion 53 when the hook body 19 and the test container 3 are arranged at a predetermined angle, and holds the hook body 19 and the test container 3 at a predetermined angle. It is configured as follows. In another expression, the shape of the opening edge 3b of the test container 3 is inclined in a direction away from the bottom of the test container as the distance from the base 17 of the hook part 15 increases. The protrusion 53 has a meshing portion 55 that meshes with the opening edge 3 b of the test container 3. The protrusion 53 closes the opening of the test container 3 when the opening edge 3 b and the engagement portion 55 are engaged with each other. The width of the tip 19 a of the hook body 19 is wider than the width of the bottom 3 a of the test container 3.

The usage method of the kit which has the said test container 3 is demonstrated using FIG. First, as shown in FIG. 18, when the test container 3 is held on a thin plate-like object 56 standing vertically, the object 56 is interposed between the hook body 19 and the test container 3 as described above. And the test container 3 can be held on this.
Next, as shown in FIG. 19, the usage method when putting the test container 3 on the desk 47 etc. is demonstrated. First, the tip 19 a of the hook body 19 is moved in a direction away from the test container 3. At this time, the protrusion 53 approaches the opening of the test container 3, and finally the protrusion 53 and the opening edge 3b come into contact with each other. At this time, the angle between the hook body 19 and the major axis of the test container 3 is a predetermined angle θ. The angle θ is preferably 20 to 60 degrees, more preferably 30 to 50 degrees. The angle θ can be determined by the angle α between the surface formed by the opening edge 3 b and the major axis of the test container 3. When the test container 3 is placed on the desk 47 in this state, the test container 3 is prevented from falling without being supported from the outside by being supported by the hook body 19. Since the width of the tip 19a of the hook body 19 is wider than the width of the bottom 3a of the test container 3, the hook body 19 can support the test container 3 stably. In the present specification, the “major axis of the test container 3” is an axis indicating the direction from the bottom of the test container 3 toward the opening of the test container 3.

  Further, when the protruding portion 53 comes into contact with the opening edge 3b, the meshing portion 55 meshes with the opening edge 3b. By this meshing, the hook body 19 and the test container 3 are fixed in a state of being held at a predetermined angle θ. For this reason, the hook main body 19 can support the test container 3 more stably. Further, the protruding portion 53 closes the opening of the test container 3 when the opening edge 3 b and the meshing portion 55 are in contact with each other. In this case, even when the test container 3 falls, the sample 23 inside the test container 3 can be prevented from leaking from the test container 3.

(8) Test container having a hook part with a spacer part FIGS. 20A and 20B are a side view and a front view of a test container 3 having a hook part with a spacer part, respectively.

  The hook portion 15 of the test container 3 is connected to the base 17 connected to the test container 3, the hook body 19 extending from the base 17 toward the bottom of the test container 3, the test container 3 and And a spacer portion 65 disposed at a position facing the hook body 19. A surface 65 a of the spacer portion 65 facing the hook body 19 is inclined with respect to the major axis direction of the test container 3. A surface 65a of the spacer portion 65 facing the hook body 19 and a surface 19b of the hook body 19 facing the spacer portion 65 are substantially parallel. The hook portion 15 is disposed near the center of the test container 3 in the long axis direction. The angle β between the surface 65a and the long axis of the test container 3 is preferably 5 to 30 degrees. Note that the surface 65 a and the surface 19 b may be open toward the tip 19 a of the hook body 19. The hook portion 15 may be disposed in the vicinity of the opening of the test container 3.

  FIG. 21 shows a usage state of the kit having the test container 3. According to FIG. 21, the test container 3 is held on the plate-like object 50 by the hook portion 15. In the present embodiment, since the hook portion 15 includes the spacer portion 65, the test container 3 is held in an inclined state. Further, the test device 1 is arranged so that the surface having the determination part, that is, the surface to which the chromatographic membrane carrier 11 is bonded faces the direction of the arrow X. During the inspection, the test container 3 is often arranged at a position lower than the inspector's line of sight, but according to the present embodiment, the test container 3 is held in an inclined state. It is easy to confirm the presence or absence of the lines 25a, 25b and the like.

(9) Test Container Consisting of Test Tool Holding Part and Sample Storage Part FIG. 22 shows an immunochromatography kit consisting of a test container consisting of the test tool holding part 57 and the sample storage part 59 and the immunochromatographic test tool 1. FIG.

  The test container 3 includes a test tool holding part 57 capable of fixing an immunochromatography test tool inside, and a sample storage part 59 capable of storing a sample therein. Are connectable to each other. The test tool holding component 57 includes the hook portion 15. In FIG. 22, the test tool 1 is fixed in the test tool holding component 57. A desiccant (not shown) is fixed in the test tool holding component 57 in order to prevent the test tool 1 from getting wet. A backflow prevention member 61 is provided above the sample addition member 7 of the test device 1 in order to prevent the sample moved to the marker holding member 9 and the like from flowing back. The backflow preventing member 61 can be formed by opening a through-hole that is slightly larger than the cross section of the test device 1 in the polystyrene foam piece. The backflow preventing member 61 is used by inserting the test device 1 into this through hole. The lower surface 57a of the test tool holding component 57 is open, and the upper surface 57b may be open or closed. When the upper surface 57b is closed, when the test tool holding component 57 and the sample storage component 59 are connected, the inside becomes a sealed space, and the sample can be prevented from flowing out in the event of falling. The sample storage component 59 has a bottomed cylindrical shape with an opening at the top, and the inner diameter thereof is approximately the same as the outer diameter of the test tool holding component 57.

Next, the usage method of the kit of this embodiment is demonstrated using FIG.
First, a predetermined amount of a sample 23 prepared by diluting a specimen such as a nasal cavity aspirate of a patient with a developing solvent is injected into the sample housing component 59. Next, the test container holding part 57 to which the test tool 1 is fixed and the sample storage part 59 are connected to assemble the test container 3. Thereafter, the test container 3 is attached to the desired object 25 using the hook portion 15. The subsequent steps are the same as described with reference to FIG.

  Next, various embodiments of the test device 1 will be described. The test device 1 may be the one shown in FIGS. 24A to 24C other than the one shown in FIG. In the test device of FIG. 24A, the sample addition member 7 is configured to cover the label holding member 9 and to contact the chromatographic membrane carrier 11. In the test device of FIG. 24B, the label holding member 9 is disposed at a distance from the chromatographic membrane carrier 11, and the sample addition member 7 covers the label holding member 9 and contacts the chromatographic membrane carrier 11. Is configured to do. In the test device of FIG. 24C, the label holding member 9 is arranged with a space between the chromatographic membrane carrier 11 and the developing member 63 is in contact with the label holding member 9 and the chromatographic membrane carrier 11. Has been placed. The unfolding member 63 can be composed of non-woven fabrics of various materials such as rayon, glass fiber or cellulose fiber, like the sample addition member 7. 24B and 24C, since a member having a high sample development speed is sandwiched between the label holding member 9 and the chromatographic membrane carrier 11, elution of the labeling substance in the label holding member 9 is performed. Speeds up and enables quick measurement.

  Various features shown in the above embodiments can be combined with each other. In the case where a plurality of features are included in one embodiment, one or a plurality of features can be appropriately extracted and used alone or in combination in the kit of the present invention.

The structure of the kit for immunochromatography of one Embodiment of this invention is shown. It is the (a) side view and (b) front view of the test device of the kit of FIG. It is the (a) side view and (b) front view of the test container of the kit of FIG. The use condition of the kit of FIG. 1 is shown. It is (a) side view and (b) front view of the test container which has another hook part with a magnet which is another embodiment of the test container of the kit of FIG. FIG. 6 shows a use state of a kit having the test container of FIG. It is (a) side view and (b) front view of the test container which has a hook part with an opening part which is another embodiment of the test container of the kit of FIG. The use condition of the kit which has the test container of FIG. 7 is shown. It is (a) side view of the test container which has a stepped hook part which is another embodiment of the test container of the kit of FIG. 1, (b) It is a front view. FIG. 10 shows a use state of a kit having the test container of FIG. (A) shows a thin object, and (b) shows a state when attached to a thick object. It is (a) side view and (b) front view of the test container which has a hook part with a grip part which is another embodiment of the test container of the kit of FIG. The use condition of the kit which has the test container of FIG. 11 is shown. It is (a) side view of the test container with a long hook main body of a hook part which is another embodiment of the test container of the kit of FIG. 1, (b) It is a front view. The use condition of the kit which has the test container of FIG. 13 is shown. It is (a) side view of the test container which is another embodiment of the test container of the kit of FIG. 1, and has a convex part in the surface facing a hook main body, (b) It is a front view. The use condition of the kit which has the test container of FIG. 15 is shown. It is (a) side view and (b) front view of the test container which has a hook part with a protrusion part which is another embodiment of the test container of the kit of FIG. FIG. 18 shows a use state when the kit having the test container of FIG. 17 is attached to a thin object. The use condition on the desk etc. of the kit which has the test container of FIG. 17 is shown. It is (a) side view of the test container which is another embodiment of the test container of the kit of FIG. 1, and a hook part has a spacer part, (b) Front view. The use condition of the kit which has a test container of Drawing 20 is shown. It is the (a) side view and (b) front view of the test container which consist of a test tool holding | maintenance part and a sample storage part based on this invention. The usage condition of the kit which has the test container of FIG. 22 is shown. FIG. 2 shows various embodiments of the test device of the kit of FIG.

Explanation of symbols

1: Immunochromatographic test tool 3: Test container 5: Base material 7: Sample addition member 9: Label holding member 11: Chromatographic membrane carrier 13: Absorbing member 15: Hook portion 17: Base portion 19: Hook body 19a: Hook Tip of main body 19b: Surface facing the spacer portion of the hook main body 21: Convex portion of the hook main body 23: Sample 25a: Blue line 25b: Red line 27: Object 29: Magnet part 31: Wall 33: Opening part 35: Wall 37: Pin 39: Thin object 41: Thick object 43: Grip part 45: Projection part 46: Object 47: Desk 49: Short plate-like object 50: Plate-like object 51: Projection part 53: Projection Part 55: Meshing part 56: Object 57: Test tool holding part 59: Sample housing part 61: Backflow prevention member 63: Member for deployment 65: Spacer part 65a: surface of the spacer portion facing the hook body

Claims (6)

An immunochromatographic test tool for detecting a substance to be detected in a sample, and a test container capable of storing the sample;
A sample adding part immersed in the sample accommodated in the test container; a label holding part for holding a labeling substance that causes an antigen-antibody reaction with the detected substance in the sample; and the detected substance And a determination unit to which an immobilizing substance that causes an antigen-antibody reaction is fixed,
The test container includes a hook portion for hooking and holding the test container,
An immunochromatography kit capable of detecting a substance to be detected in a state where the test container is held by the hook portion. The test container has a bottomed cylindrical shape having an opening at the top,
The kit according to claim 1, wherein the test device is used by being inserted from an opening of the test container. The kit according to claim 1, wherein the hook portion includes a base portion connected to the test container and a hook body extending from the base portion toward a bottom direction of the test container. The hook portion includes a base connected to the test container, a hook main body extending from the base toward the bottom of the test container, a position connected to the test container and facing the hook main body. The surface of the spacer portion that faces the hook body is inclined with respect to the major axis direction of the test container. Kit. The test container includes a test tool holding part capable of fixing the test tool therein, and a sample storage part capable of storing the sample therein, and the test tool holding part and the sample storage part are connected to each other. The kit according to any one of claims 1 to 4, which is possible. A test container for storing a sample in which a substance to be detected is detected by an immunochromatographic test device, comprising a hook portion for hooking and holding the sample, with the hook portion being held by the hook portion A test container that can detect the target substance.

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