JP2007070266A - Agent for suppressing increase of blood glucose level - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an agent for suppressing increase of a blood glucose level, capable of being utilized as medicines such as oral medicine, suppository and drops and foods, etc. <P>SOLUTION: The agent for suppressing increase of the blood glucose level comprises litchi seed or its extract. The oral medicine comprises the agent for suppressing increase of blood glucose level. A beverage or a food comprises the agent for suppressing increase of the blood glucose level. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a lychee seed-derived blood sugar level increase inhibitor applied to, for example, pharmaceuticals such as internal medicines, suppositories, and injections, quasi-drugs, and foods and drinks.

  As a conventionally used one, there is an unaffected child, which is the same family as the lychee plant. Unaffected children used the pericarp as a soap substitute. In addition, lychee may be traced to the traditional Chinese bureaucratic test, and once in Beijing, when a newborn baby was born on the third day after birth, lychee, longan, He always throws in walnuts, wishes to be the head of the three exams in the department, and expresses his congratulations. Lychee has been prized as an edible since the days of the princess, but seeds were never edible. Also, lychee seeds are used as an ointment for skin diseases. Issued on November 10, 1978, published by World Fruit Tree Book and Agricultural Books Co., Ltd. Japanese Patent Laid-Open No. 2000-128730 shows a patent using lychee (lychee) as a moisturizing agent.

As described above, it is a well-known fact that lychee seeds are used for skin external preparations and baths, and there is no novelty to use lychee seeds and fruits as skin external preparations.
However, lychee seed has never been used as a medicine such as an internal medicine, a suppository or an injection, or a food. The present invention has found extremely useful effects such as suppression of an increase in blood glucose level in lychee seeds.

That is, the blood sugar level elevation inhibitor of the present invention contains litchi seeds or an extract thereof.
Moreover, the lipid metabolism improving agent of the present invention is characterized by containing litchi seeds or an extract thereof.
Furthermore, the chemical | medical agent of this invention contains the said blood glucose level raise inhibitor.
Furthermore, the food and drink of the present invention is characterized by containing a lipid metabolism improving agent.

Hereinafter, embodiments of the present invention will be described in detail.
The blood glucose level elevation inhibitor of the present invention is limited to using lychee seeds, and all effects can be expected if the method uses lychee seeds. Although lychee seeds may be used as they are, when using an extract, a method using a polar organic solvent is a better method. As a solvent to be used, water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, ethyl acetate, ether, hexane, carbon dioxide or the like is used as a single solvent as it is. Two or more types can be arbitrarily mixed and used to create an extract.

  When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C., preferably about 80 to 100 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used. Preferably, deep water (deep ocean water) is used as the extraction solvent.

  When hydrous ethanol is used as the extraction solvent, the ethanol concentration is preferably 30 to 90% (volume ratio). This is because if the amount is less than about 30% (volume ratio) or exceeds 90% (volume ratio), the extraction amount of the active ingredient tends to decrease. The extraction temperature is 20 to 80 ° C, preferably about 50 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  As a method for extracting the blood sugar level increase inhibitor of the present invention, any method such as continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction can be employed, and any device is used at room temperature or under reflux heating. be able to.

As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or water-containing ethanol is used as the extraction solvent, the extraction is performed for about 30 minutes to 5 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying, and purification in accordance with a conventional method to obtain a blood sugar level increase inhibitor.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  Moreover, the blood sugar level rise inhibitor of this invention can be contained in food-drinks. For example, confectionery (gum, candy, caramel, chocolate, cookies, snack confectionery, jelly, gummy, tablet confectionery, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasoning (Miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and other general and health foods (tablets, capsules, etc.), nutritional supplements (nutrient drinks, etc.) ) Etc. You may add the blood glucose level raise inhibitor of this invention to an instant foodstuff. For example, a product obtained by spray-drying or freeze-drying a blood sugar level increase inhibitor together with powdered cellulose can be easily contained in a food or drink by making it into a powder, granule, tablet or solution.

The blood glucose level elevation inhibitor of the present invention can be contained in pharmaceuticals used for oral administration (including pharmaceuticals and quasi drugs in the Pharmaceutical Affairs Law). For example, it can be in the form of products such as soft / hard capsules or tablets, granules, fine granules, powders, liquids and the like.
Moreover, you may administer the blood glucose level raise inhibitor of this invention parenterally. When administered as a parenteral preparation, it can be in the form of an injection, a suppository, or the like in the form of a solution or with a dispersion, suspension, stabilizer or the like added.

[Production of lychee seed extract]
As the raw material, lychee seeds were used after being dried and ground. First, lychee seeds were refluxed with 60% (v / v) water-containing ethanol to obtain a water-containing ethanol extract. At this time, the reflux temperature was 75 to 85 ° C., and the reflux time was 4 hours. Thereafter, the same amount of dextrin as the solid content of the concentrated solution was added to obtain a dry powder to obtain a lychee seed extract (Example).

  In order to examine the functionality of the manufactured lychee seed extract, the following animal experiments were conducted.

  Rats were fed a diet containing the produced lychee seed extract. For comparison, rats were fed a diet that did not contain lychee seed extract.

  Male Otsuka Long-Evans Tokushima Fatty rats (hereinafter referred to as OLETF rats) that genetically spontaneously develop one-month-old type 2 diabetes, and male Long-Evans Tokushima Otsuka rats (hereinafter referred to as diabetes) that do not develop diabetes in the control model animals. LETO rat). Rats were housed in an animal breeding room with a light cycle of room temperature 22 ± 1 ° C., room temperature 55 ± 5%, 8: 00 &#12316; 20: 00.

  During the first 3 months, MF chow diets were given to LETO and OLETF rats for preliminary breeding. Since OLETF rats usually develop type 2 diabetes from 5 to 8 months of age, we started eating the test meal together with LETO rats from 4 months of age one month before the possibility of onset.

  After 4 hours of fasting for 6 hours (9:00 &#12316; 15:00), blood was collected from the tail vein, blood glucose level was measured, and grouped into 6 animals per group so that body weight and blood glucose level were equal Then, the following test meals were fed freely for 5 months.

  The test diet was a purified diet based on AIN-76, which was fed to a control group of LETO and OLETF rats. Weight composition (g / kg) of feed is 200 casein, 100 corn oil, 35 mineral mixture (AIN-76-MX), 35 vitamin mixture (AIN-76-VX), 50 cellulose, 2 choline bitartrate, DL- Methionine 3, corn starch 150 and sucrose 450 were used. In the lychee seed extract-added food, lychee seed extract was added at 5% of the total weight of the feed, and the amount of sucrose was reduced by the added amount. During the breeding period, food intake was measured every day, and body weight and water intake were measured every other day.

  1, 2, 3, 4 and 5 months after the start of test meal administration, blood glucose was measured by blood collection from the tail vein after 6 hours of fasting using a blood glucose test measurement Medisafe chip (Terumo, Tokyo). FIG. 1 shows the change in blood glucose level in rats over 5 months after taking the test meal.

  As shown in FIG. 1, the blood glucose level of LETO rats that did not develop diabetes who took the control diet was low throughout the breeding period. On the other hand, the blood glucose level of OLETF rats that developed diabetes that took the control diet increased over time, and was considered to have developed diabetes. The blood glucose level of OLETF rats fed with lychee seed extract did not rise significantly during the breeding period, and remained at a significantly lower level than that of OLETF rats fed with a control diet. From this, it became clear that the lychee seed extract has an excellent blood glucose rise inhibitory effect.

Serum insulin concentration in OLTF rats fed control diets decreased 5 months after the onset of diabetes, but insulin concentrations in OLETF rats fed lychee seed extract showed the same high level as LETO rats. The results are shown in Table 1. It was observed that lychee seed extract inhibits the decrease in insulin secretion by inhibiting the functional damage of pancreatic islets of Langerhans due to the onset of diabetes.

Serum and liver triglyceride levels in OLETF rats fed the control diet were markedly elevated in LETO rats, but lychee seed extract effectively reduced serum and liver triglyceride concentrations in OLETF rats. The results are shown in Table 2.

Lychee seed extract reduced serum and liver cholesterol levels. The results are shown in Table 3.

Hereinafter, the example of the compounding example of the blood glucose level raise inhibitor (Lychet seed extract) of this invention is given. However, the following blending examples do not limit the invention of the present invention, and it is needless to say that the blending examples are not limited to the following blending examples as long as they have an effect of suppressing an increase in blood sugar level.
Formulation Example 1: Chewing gum
53.0% by weight sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Lychee seed extract 0.5
100.0% by weight

Formulation Example 2: Gummy
Reduced water tank 40.0% by weight
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Ume Juice 4.0
Ume Flavor 0.6
Dye 0.02
Lychee seed extract 1.0
100.0% by weight

Formulation Example 3: Candy
50.0% by weight sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Lychee seed extract 0.4
100.0% by weight

Formulation Example 4: Yogurt (hard / soft)
41.5% by weight of milk
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Lychee seed extract 0.4
Perfume
Water residue
100.0% by weight

Formulation Example 5: Soft capsule
Brown rice germ oil 87.0% by weight
Emulsifier 12.0
Lychee seed extract 1.0
100.0% by weight

Formulation Example 6: Coffee beverage (liquid)
Roasted coffee beans 6.0% by weight
Sugar 6.0
Baking soda 0.2
Emulsifier 0.15
Lychee seed extract 1.0
Water residue
100.0% by weight

Formulation Example 7: Coffee drink (powder)
Instant coffee 90.0% by weight
Skim milk 7.0
Lychee seed extract 3.0
100.0% by weight

Formulation Example 8: Soft drink
Fructose glucose liquid sugar 30.0% by weight
Emulsifier 0.5
Lychee seed extract 0.05
Perfume
Purified water residue
100.0% by weight

Formulation Example 9: Tablet
Lactose 54.0% by weight
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Lychee seed extract 1.0
100.0% by weight

Formulation Example 10: Tablet
76.4% by weight of sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Lychee seed extract 0.5
Purified water 3.9
100.0% by weight

  As described above, the lychee seed extract can be sufficiently used as a health food or a pharmaceutical having a possibility of exerting an effect for preventing or improving diabetes and improving lipid metabolism.

In the present Example, it is a graph which shows the change of the rat blood glucose level for 5 months which ingested the test meal.

Claims (3)

  A blood sugar level increase inhibitor comprising litchi seeds or an extract thereof.   2. A pharmaceutical comprising the blood glucose level elevation inhibitor according to claim 1. A food / beverage product comprising the blood sugar level increase inhibitor according to claim 1.

Images