JP2007068933A - Shoe insole with vegetable essential oil for preventing and treatment for foot trichophytosis - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an inexpensive, simple and preventive implement for treatment of a patient, surely producing an effect and attaining high safety to foot trichophytosis, which a number of patients suffer from and which is easy to relapse. <P>SOLUTION: In the case of interdigit type foot trichophytosis, when the diseased part is limited to the sole side, at least a shoe insole applied to that part is supplied with vegetable essential oil to obtain a satisfactory effect. However, when the diseased part is extended to the instep side, a cover is added to the shoe insole to supply both sides with vegetable essential oil to surely produce the effect. Further, a cloth piece of another material quality different from the body is stuck to the part of the shoe insole and the cover corresponding to the vicinity of the diseased parts, and the cloth piece is supplied with vegetable essential oil to further enhance the anti-trichophytosis effect. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method and apparatus for preventing or treating tinea pedis caused by ringworm.

  Ringworm fungus invades and develops keratinized skin tissue, that is, the stratum corneum, hair and nails, and causes local infection (ringworm). Ringworm is the most prevalent of all human diseases, and it is said that more than 10% of the population is constantly affected in almost all parts of the world. In Japan, the number of patients is estimated to exceed 10 million. Has been. The largest number of patients is tinea pedis, which is commonly referred to as “athlete's foot”, and the most frequently seen type is the intercostal type, especially between the 4th and 5th. Treatment of ringworm, except for internal use in onychomycosis, is generally performed by a so-called external application agent that directly contacts the affected part with the drug, but ringworm is highly resistant to the drug and is likely to repeat recurrence. In addition, ringworm bacteria are resident in some humans and pet animals and easily cause reinfection in other humans. Because of these characteristics, the prevalence of ringworm remains at a high level. Ringworm is caused by infection with fungi of the genus Trichophyton, Epidermophyton, and Microsporum, and the causative bacteria include Trichophyton rubrum, T. et al. Mentagrophytes followed by Epidermophyton floccusum.

  A method and apparatus for preventing or treating tinea pedis using its vapor without directly contacting the plant essential oil having anti-tinea fungus activity directly (patent publication 2000-128800). , Infection prevention and / or treatment of athlete's foot and its equipment). However, with regard to the steam of plant essential oil, the effective range from the location of the plant essential oil is examined in detail, and based on this, the arrangement of the plant essential oil is devised, and this is true for shoe insoles that can reliably exert preventive or therapeutic effects against foot ringworm. It was not reported until.

  An object of the present invention is to devise the arrangement of plant essential oil having anti-tinea activity for the prevention and treatment of foot tinea, which has the largest number of patients, and is a safe and effective, inexpensive and simple shoe insole Is to provide.

  Ringworms are aerobic and grow near the surface of the skin. The main component of the plant essential oil vapor is a hydrophobic, low molecular weight terpene compound, which easily penetrates into the skin surface to reach ringworm. Ringworms and spores are highly lipophilic and adsorb and absorb hydrophobic plant essential oil components well. Thus, the plant essential oil component absorbed by ringworm fungus suppresses its growth and further sterilizes and exhibits its effect. However, in order for the plant essential oil component to evaporate to reach the affected area of tinea which is far from the location, and to exert effective anti-tinea fungi activity on the spot, the distance between the two is limited to a certain range. It has not been examined in detail until. The present inventors made a plastic human foot mold, made several shallow holes in it, embedded an agar medium in which ringworm bacteria were grown, and put on leather shoes with socks and a shoe insole on which plant essential oil was placed. I tried a model experiment. As a result, if the distance from the plant essential oil location to the affected area of tinea is within 1.5 cm, a sufficient effect for prevention and treatment will be exhibited, but a sufficient distance will not be obtained if the distance is longer. The headline has led to the present invention. In addition, the plant essential oil in this invention means the aromatic oily substance obtained by steam distillation, pressing or solvent extraction from plant materials, such as an herb.

  The present invention is a shoe insole in which the plant essential oil is devised so that the plant essential oil vapor having anti-tinea fungi activity is effective against tinea pedis, and it can be effectively prevented or prevented by using it. Can be treated. In addition, it is safe, inexpensive and convenient, and is easy for patients to use on a daily basis. They can contribute to the prevention and treatment of tinea pedis which is likely to recur.

The present invention is a shoe insole in which the plant essential oil having anti-tinea fungi activity is devised so as to obtain a sufficient effect. The most common tinea pedis is the intercostal type, but when the affected area is confined to the sole of the foot, it can be prevented or treated with a shoe insole in which plant essential oil is placed at least in the area including the affected area.
However, if the affected area also extends toward the instep, it is necessary to dispose the plant essential oil in the vicinity thereof, and one method is to cover the instep with the plant essential oil added. Further, as shown in the examples, the diffusion of the essential oil vapor tends to exert the antibacterial effect more than the case of diffusing upward, and it is more effective to add a cover. Further, it is desirable that the cover is easily removable from the shoe insole body, and the old cover which has been reduced in effect thereby can be replaced with a new one, and a high effect can be obtained again. In other words, it is important to select a shoe insole suitable for the spread of the affected area of foot tinea, which makes it possible to reliably prevent or treat tinea pedis.

  The plant essential oil that can be used in the present invention can be used as long as it has an antibacterial effect. Examples of particularly strong anti-tinea activity include the following. Clove oil, oregano oil, tea tree oil, lavender oil, geranium oil, perilla oil. One or more plant essential oils are selected from these and placed on the shoe insole and cover. In some cases, a plant essential oil that is fragrant and has a deodorizing effect, such as peppermint oil, may be added simultaneously.

The arrangement of the plant essential oil used in the present invention can be easily performed by spraying the shoe insole portion and the cover including the region in the vicinity of the affected area of the ringworm with a spray. As a spraying method, a solution spray in which plant essential oil is contained at an appropriate concentration, an aerosol spray in which plant essential oil is directly dissolved in a liquefied gas, and the like can be used. The shoe insole portion and the cover on which the plant essential oil is disposed can be pasted with a cloth made of a material different from that of the main body to improve the ability to retain the plant essential oil, thereby further enhancing the effect.
In addition, the plant essential oil used in the present invention may be microencapsulated and sprayed on a shoe insole and a cover, thereby improving the durability of the effect.

  The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

  Example 1. Number of small conidia and colony size after growth of inoculated ringworm

Trichophyton T. cerevisiae preserved at Teikyo University Medical Mycology Research Center mentagrophytes TIMM2789 is used as an inoculum and inoculated into a 1/10 Sabouraud glucose slant medium and cultured at 27 ° C. for 1 week, the fungus grows and produces small conidia. A physiological saline containing 0.05% Tween 80 is added and suspended in the medium in which the small conidia are produced, and a supernatant containing the small conidia is prepared. The supernatant is diluted with physiological saline, and the contained conidia are adjusted to the following number. 10 ⁸ , 10 ⁷ , 10 ⁶ , 10 ⁵ , 10 ⁴ , 10 ³ cells / ml.
Inoculate 100 μl of the adjusted conidia suspension on a flat plate prepared by dispensing Sabouraud's glucose agar medium into a sterile petri dish (diameter: 8.5 cm, Eiken Chemical, Tokyo) Spread paint. After culturing at 27 ° C. for 2 days, the portion of the flat plate where the mycelium has uniformly grown is shown as cork borer NO. 3 (inner diameter 7 mm) is punched out and the agar block with the mycelium attached is cut out.
Cut out 3 agar blocks where mycelia grew from a flat plate with the same number of conidia, and prepared them by dispensing 65 ml of Sabouraud Agar Medium into Eiken Sterilized No. 1 Petri dish (230 × 80 × 15.5 mm). Then, each is placed with the mycelium side up, and cultured at 27 ° C. for 4 days.

Table 1 shows the initial number of small conidia and the average size of three colonies grown from the same number of conidia. The initial number of small conidia and the average colony size showed a positive correlation, and they were plotted on a semilogarithmic graph to create a calibration curve. In particular, a linear relationship was obtained when the number of conidia was between 10 ^{4 and} 10 ⁶ . That is, it was possible to estimate the initial viable cell count by measuring the colony size.

  Example 2 Measurement of anti-tinea fungi activity of plant essential oil vapor using sealed box

Use a rigid plastic box (Jalee, Ltd. Tokyo) with an internal volume of 1.3 l as a sealed box, put a sterilized glass dish (diameter 9 cm) in it, spread sterilized filter paper (diameter 8 cm), and add 3 ml of sterilized water . On top of this, three agar blocks (prepared from a plate inoculated with 10 ⁷ small conidia) grown with ringworm fungi are placed with the mycelia facing up. Fold sterile filter paper (diameter 9 cm) so that the diameter intersects the crease at a right angle of 8: 2, and puts the large part vertically on a sterile glass dish. A certain amount of plant essential oil is applied to the tip of the raised portion. When the amount is 20 mg or less, it is dissolved in ethyl acetate in advance and applied so that the plant essential oil becomes a certain amount.
Put the ringworm and plant essential oil in this way, seal the box, and store at 27 ° C. for 24 hours. Thereafter, three agar blocks are taken out, and the mycelia are transferred to a Sabouraud agar plate prepared separately in a No. 1 petri dish (6 agar blocks per dish) and cultured at 27 ° C. for 4 days. The colony size of ringworm fungus grown from the block is measured using calipers, and the viable cell count is calculated from the average of the three measured values using the calibration curve created in Example 1. Furthermore, the number of viable bacteria remaining after exposure to the essential oil vapor is divided by the number of viable bacteria in the untreated group and multiplied by 100, and the resulting viability (%) is calculated. expressed.

  Table 2 shows the anti-tinea fungus fertility of each plant essential oil expressed in terms of fungus survival rate. Oregano oil showed the strongest effect in the smallest amount, followed by clove oil, perilla oil, geranium oil, lavender oil, and tea tree oil, all of which showed remarkable effects.

  Example 3 Effect of diffusion direction on anti-tinea activity of plant essential oil vapors

  As in Example 2, the effect of the diffusion direction on the anti-tinea fungi activity of plant essential oil vapor was examined by a method using a sealed box. In other words, when examining the effect of steam diffusing downward, place a glass dish with an agar block with ringworms in a sealed box, install a wire mesh on it, and filter paper coated with plant essential oil on the glass dish. Placed directly above. In order to examine the effect of the steam diffusing upward, a glass dish with an agar block was placed on the installed wire mesh, and a filter paper coated with plant essential oil was placed directly under the glass dish. After storing at 27 ° C. for 3 to 24 hours, the agar block was taken out to examine the viability of ringworm. The results are shown in Table 3.

  As shown in the example of oregano oil, down-diffusion showed stronger activity (lower survival rate) after 3 hours exposure even at the same distance. However, after 24 hours of exposure, the survival rate was significantly reduced, and the difference between the two could not be detected. In the example of tea tree oil, in the case of upward diffusion, the shorter the distance, the stronger the activity. However, downward diffusion showed stronger activity. In the case of perilla oil, the addition amount in this experiment showed strong activity, and a difference depending on the diffusion direction could not be detected. In summary, when the amount of plant essential oil added and the exposure time are set to be the same, the downward diffusion of steam shows stronger activity than the upward diffusion. In addition, the closer the distance between the plant oil location and the ringworm, the stronger the activity.

  Example 4 Evaluation of anti- ringworm fungus activity of plant essential oil vapor using foot-shaped model and shoes

Make a hard plastic foot model with several holes (diameter 1cm, depth 1cm), and after sterilization, each hole is filled with absorbent cotton to a thickness of 5mm, and sterile water is injected into the hole. To prevent drying). On top of that, an agar block in which ringworm fungus was grown was put with the mycelium side outward, and a commercially available nylon sock was put in cowhide boots. Cork borer NO. 2 (inner diameter 10 mm), a hole was made, and a paper disc (diameter 8 mm) to which vegetable essential oil was added was placed and previously laid on boots. The hole of the foot model and the shoe insole was adjusted and adjusted so that the distance between the essential oil location and the ringworm was constant so that the influence of the distance of the essential oil vapor effect could be quantitatively evaluated. The amounts of various plant essential oils added to the shoe insole were determined based on the test results using a sealed box, assuming that the shoe internal volume was 1 liter.
The shoes containing ringworm and plant essential oil are stored at 27 ° C for 6 to 24 hours. The agar block with the ringworm is removed from the foot model and transferred to a newly prepared Sabouraud agar plate (No. 1 square petri dish). did. As in Examples 2 and 3, the agar block was cultured at 27 ° C. for 4 days, and the initial viable cell count was calculated from the grown colony size. %) And used as an index of effect. The bacteria survival rate (%) was determined to be effective when it was 10% or less.

  Example 4.1. Trials when the ringworm is placed on the back of the foot model

  In this test, an agar block in which ringworm was grown was placed in two cylinders on the back side of the foot model and near the base of the toes. Add tea tree oil (8 or 20 μg / paper disk), oregano oil (1 or 5 μg / paper disk), clove oil (2 or 10 μg / paper disk), and geranium oil (4 or 20 μg / paper disk) at the same level. Two paper discs were placed near the base of the toes of the shoe insole. As a result, the effect judged to be effective was not obtained with any plant essential oil. That is, unlike the result expected from the test of the sealed box, it was considered that the plant essential oil placed on the shoe insole hardly exerted an effect on the affected part of the tinea on the back side of the foot.

  Example 4.2. Test 1 when ringworm is placed on the back of the foot model

  In this test, a foot model having three holes on the back side (FIG. 1A) was used, and an agar block on which ringworm was grown was placed at two locations at the base of the toes and at one location on the arch. The plant essential oil was placed at two locations on the shoe insole so as to hit the agar block at the base of the toes. In addition, the distance from the plant essential oil arrangement | positioning location to the agar block of the arch part was set to 12 cm. The results are shown in Table 4. In the case of a low dose, all essential oils were ineffective after 6 hours of exposure, but at a high dose (10 times the low dose), tea tree oil and oregano oil were effective against ringworms at the base of the toes. All other essential oils, except oregano oil, were effective at low doses after 24 hours exposure. However, all essential oils were ineffective against tinea tinea. The above results show that the essential oil is effective only when it is placed in the vicinity of ringworm.

  Example 4.3. Test 2 with ringworm bacteria on the back of the foot model

In this test, a foot model having three holes on the back side was used as in Test 1, but the arrangement method of the plant essential oil was changed. That is, a piece of filter paper of about 40 cm ² cut out in accordance with its shape was placed on the toe portion of the shoe insole, and the essential oil dissolved in ethyl acetate was uniformly applied to the whole. The test results are shown in Table 5. All the essential oils were effective at low doses, except for low-dose oregano oil, against ringworms at the base of the toes. However, all of the essential oils were ineffective against the tinea rotumans part of the arch approximately 7 cm away from the end of the filter paper coated with essential oil. The above results show that the essential oil is not spot-disposed in a narrow area corresponding to the ringworm, but is effective even if the essential oil is arranged in an area of several tens of cm ² including the area. This shows that the effect is not recognized when the distance is too far.

  Example 4.4. Test 3 with ringworm bacteria on the back of the foot model 3

  In this test, a foot-shaped model (FIG. 1B) having 8 holes centered on the toe root on the back side was used, and an agar block on which ringworm was grown was placed on each. The plant essential oil was applied to each of three paper discs in a set amount, and three plant oils were arranged around the base of the toes of the shoe insole. A foot type model in which socks were put on leather shoes with an insole was put in, and it was stored at 27 ° C. for 24 hours to determine the effect. The test results are shown in Table 6. All essential oils tested were effective against all nearby ringworms, with the exception of ringworms located away from where they were placed. For example, in the case of tea tree oil, the trichophyton, NO. ,,,, Ringworm, NO. It was also effective for 2, 3, 5, and 6. However, the tinea fungus far away is NO. No effective effect was obtained for 8. In particular, perilla oil exerted a strong anti-tinea effect. Examining the distance between the ringworm fungus and the location where the essential oil was placed, and determining the respective distances in combination with the effects, the effects were definitely evaluated as effective if the distance between the two was within 1.5 cm. When it was further away, the effective effect was not necessarily obtained. From the above results, it was concluded that in order to obtain an effective effect with a relatively small amount of essential oil as in this test, it is necessary to keep the distance between the location of the essential oil and ringworm bacteria within 1.5 cm.

  Example 5.1. Evaluation by foot model when plant essential oil is sprayed on shoe insole 1

A preparation for spraying was prepared by the following method. Blend essential oils each containing 3.5% tea tree oil, 3.5% lavender oil, 1.0% geranium oil and 2.0% peppermint oil were prepared in advance, respectively, 70% ethanol 80%, isopropanol 15%, glycerin 5 % Dissolved in a mixed solvent consisting of 5%. After spraying this preparation on the tip portion (about 25 cm ² ) of the shoe insole, the shoe insole was air-dried at room temperature for 5 minutes and laid on leather shoes. The amount of spray at one time was 0.8 ml and sprayed 2 to 5 times. In this test, a foot-shaped model (FIG. 1A) having three holes on the back side was used, and an agar block of ringworm fungus growing in each hole was placed and socks were put on. It was put into a leather shoe laid with a shoe insole and stored at 27 ° C. for 24 hours to determine the effect. The test results are shown in Table 7. The anti-tinea fungus effect was stronger at the tip, and the effect became stronger as the number of sprays increased.

  Example 5.2. Evaluation by foot model when plant essential oil is sprayed on shoe insoles 2

  The shoe insole used in this experiment was used by placing a filter paper (about 25) on the front end of the main body with an aluminum foil in between and fastening the periphery with staples. The spray formulation was prepared as in Example 5.1. After spraying this formulation on filter paper, the shoe insole was air-dried at room temperature for 5 minutes and laid on leather shoes. The spray amount and number of spray formulations, the foot model used, and the storage conditions were the same as in Example 5.1. The test results are shown in Table 8. The anti-tinea fungus effect at the tip was markedly stronger compared to the result of Example 5.1. This result shows that the anti-tinea effect can be greatly improved by the material of the dough holding the plant essential oil. Further, when the insole was sprayed before being put into the shoes, the plant essential oil was limitedly arranged, and a more preferable effect was obtained.

  The present invention is a shoe insole in which the arrangement of plant essential oil is devised so that the plant essential oil vapor having anti-tinea fungus activity actually exerts an effect against tinea pedis, and it is effective in using it to effectively treat foot tinea. Prevention or treatment becomes possible. In addition, the present invention is highly safe, inexpensive and simple, and easy for patients to use on a daily basis. For the above reasons, the present invention can contribute to the prevention and treatment of tinea pedis which has a large number of patients and is likely to recur, and has high industrial utility value.

A is a photograph of a foot model with 3 holes on the back side and cowhide boots containing it (used in Examples 4.2 and 4.3). B is a photograph of a foot model with 8 holes on the back side and cowhide boots containing it (used in Example 4.4).

Explanation of symbols

  The numbers A and B in FIG. 1 indicate the numbers of holes formed in the foot model.

Claims (6)

The plant essential oil having anti-tinea fungus activity is placed in an area within 1.5 cm from the affected area or the site where infection is to be prevented without directly contacting the skin, thereby treating or preventing foot tinea Shoe insoles The shoe insole according to claim 1, wherein a plant essential oil having anti-tinea activity is disposed on at least a part including a region corresponding to a toe and a base thereof, thereby treating or preventing foot ringworm. The shoe insole according to claim 1 or 2, wherein a fabric made of a material different from the main body is attached to a region where the plant essential oil having anti-tinea fungi activity is disposed, thereby treating or preventing foot tinea. The shoe insole having a cover covering the back side of the foot from the base of the toes to the toes according to Claims 1, 2, and 3, wherein a plant essential oil having anti-tinea fungus activity is also disposed on the cover Shoe insoles for the treatment or prevention of tinea pedis The plant essential oil according to claim 4, wherein the cover is replaceable and is made of a material different from the main body and having anti-tinea activity, thereby treating or preventing foot tinea Features shoe insoles 6. The plant essential oil having anti-tinea activity in claim 1, 2, 3, 4 and 5, at least one selected from clove oil, oregano oil, tea tree oil, lavender oil, geranium oil and perilla oil Shoe insoles for the prevention and treatment of foot ringworm, characterized by

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