JP2007051120A - Method of manufacturing mulberry branch and stem extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a mulberry branch and stem extract enhanced in blood sugar suppresive effect by synergetic effect of an activity inhibition of an α-glucosidase by a 1-deoxynojirimycine and an activity inhibition of an α-amylase by polyphenols. <P>SOLUTION: By squeezing mulverry chips which have been conventionally diposed of by a compressor, the mulberry branch and stem extract containing the 1-deoxynojirimycine and polyphenols in a high concentration and having the high blood sugar suppressing effect, is extracted. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

Detailed Description of the Invention

A technology that has been found to have an inhibitory effect on the increase in blood sugar level of mulberry chip extract, which has been incinerated as waste for the purpose of preventing diabetes and other lifestyle-related diseases caused by saccharides ingested in daily diets.

The lifestyle-related diseases seen in Japanese people in recent years have been attributed to the Western diet, but the daily diet is largely reflected and may be due to excess sugar due to ingestion of rice and grains. high. Diabetes mellitus, in particular, is the most preventive and therapeutic means of improving dietary habits, and is being guided by specialized institutions such as low-calorie diets, exercise therapy, and drug therapy.

Recently, the development of drugs that suppress the absorption of saccharides or regulate the degradation metabolism of saccharides has progressed, and “Acarbose”, “Baisein”, etc. have been put on the market as α-glucosidase inhibitors, and now they are mainly used as therapeutic and preventive agents for diabetes and obesity. There is a feeling. It was used as a herbal medicine for diabetes and hypertension as “mulberry skin”, and many of its actions were related to the inhibitory action on the increase in blood glucose level. Since then, it has been clarified that the mechanism of the inhibitory action on the increase in blood glucose level is based on the α-glucosidase inhibitory action by 1-deoxynojirimycin (YOSIKUNI, Y., Agric. Biol. Chem. 52, pp, 1988). It was done. In recent years, α-amylase inhibitory action, maltase, sucrase inhibitory action has been found in heat-extracted extracts with respect to mulberry leaves, and it has been suggested that the active substance is a component other than 1-deoxynojirimycin. 1-deoxynojirimycin is found only in the culture solution of plants of the genus Zacobinia from Latin America and actinomycetes except for mulberry. Therefore, it is clear that mulberry has a specific inhibitor of blood sugar level elevation that is not found in other plants.

Problems to be invented

The object of the present invention is to clarify in the present invention that a mulberry bark extract has the same blood glucose level inhibitory substances as mulberry bark and mulberry leaves that have been reported in the past. There is a possibility that it will develop as a functional health food that is very useful for the improvement and prevention of the onset of secondary diabetes, for which an increase in blood glucose level is a concern.

Mulberry strips produced from mulberry strains in the spring were harvested 2-6 months later, and 20 kilograms dried for 1 to 12 months were crushed into chips of about 0.5 to 5 centimeters. After this was immersed in water, an extract of 10 liters was obtained by squeezing with a compressor. 10 liters of extract obtained by immersion compression was continuously concentrated in a hot bath at 85 ° C. or less to obtain 500 ml of concentrated extract.
Determination method of 1-deoxynojirimycin

A solution obtained by accurately taking 0.5 ml of the soaked compressed extract and concentrated extract in a capped screw-cap test tube and dissolving 2.4 ml of 30 mM borate buffer and 5.0 mg of 4-Fluoro-7-nitrobenzofurazane in 1 ml of ethanol 100 Add exactly microliters and mix. The mixed solution was heated at 60 ° C. for 40 minutes and then immediately cooled, and 2 ml of 1N hydrochloric acid was accurately added. 20 microliters of this mixture was analyzed by HPLC. For analysis, an HPLC system (model LC-10A) manufactured by xxx was used, the column was an ODS column (YMC-pack DOS-A A-302), the moving bed was liquid A; 0.05% phosphoric acid, liquid B Analyzed with methanol: 5% phosphoric acid (99: 1) with a 0 minute (A100%)-45 minute (95%, B5%) gradient system (flow rate 1 milliliter l / min, temperature 40 ° C.); The fluorescence intensity was measured and obtained at Ex 470 nm and Em 530 nm.
Quantitative determination of total polyphenols

The total polyphenol was quantified by a colorimetric method using a phenol coloring test tablet (Wako Pure Chemical Industries, Ltd.). One phenol coloring test tablet was added to 10 milliliters of the test solution and allowed to stand at room temperature for 20 minutes, and then the absorbance at 510 nm was measured and calculated as the amount of caffeic acid.

阿武らによって報告されている（日本食品保蔵科学会雑誌ＶＯＬ．３０ Ｎｏ．５，２００４）桑葉エキス中に含まれる１−デオキシノジリマイシンの含有量に比べ、桑条浸漬抽出エキス中の１−デオキシノジリマイシン含有量の方が勝る結果が得られた。また、総ポリフェノール含量も桑葉エキスの報告内容を上回る成績が得られた。この成績は、桑葉エキスを凌駕する血糖値上昇抑制効果が桑条浸漬抽出エキスにあることを示唆している。Content of 1-deoxynojirimycin and total polyphenols in mulberry trunk soaked extract and concentrated extract

Compared with the content of 1-deoxynojirimycin contained in the mulberry leaf extract reported by Abu et al. (Japan Food Preservation Science Society magazine VOL.30 No. 5, 2004), A better result was obtained with a deoxynojirimycin content. In addition, the total polyphenol content exceeded the reported content of mulberry leaf extract. These results suggest that the extract extracted from mulberry leaves has the effect of suppressing the increase in blood glucose level that exceeds that of mulberry leaves extract.

投与１０分後から血糖値が１００％以上上昇した無処置対象群に対して、桑条浸漬エキス群および濃縮エキス群では共々血糖値はほとんど上昇せず１００分後まで検体投与前の血糖値とほぼ同じ値で推移した。以上の結果は１−デオキシノジリマイシンによるグルコシダーゼの阻害のみならず、ポリフェノール類によるアミラーゼの阻害が起こったことを示している。Inhibition of blood glucose elevation by oral administration of mulberry strip soaked extract and superheated concentrated extract Using 7-week-old Wister male rat fasted overnight the day before the experiment, before mulberry strip soak extraction before administration of artificial feed containing starch and sucrose The extract and the heat-concentrated extract were orally administered, and the blood glucose level of each group of 5 mice was measured over time by the glucose oxidase method, and the increase inhibition rate was calculated from the average value, and compared with the extract non-administered group. The change rate (%) of the blood glucose level was obtained from the following formula.
Change rate of blood glucose level (%)
= Blood glucose level after sample administration (mg / dl) / blood glucose level before sample administration (mg / dl) x 100

In contrast to the untreated subject group, whose blood glucose level increased by 100% or more from 10 minutes after administration, the blood glucose level hardly increased in the mulberry strip soaked extract group and the concentrated extract group. It remained almost the same value. The above results indicate that not only the inhibition of glucosidase by 1-deoxynojirimycin but also the inhibition of amylase by polyphenols.

The invention's effect

Since 1-deoxynojirimycin and polyphenol contained in the mulberry extract extract are water-soluble, it is easy to adjust the concentration of the active ingredient, and the drinking water can be used from the base to the daily confectionery. That is, the blood sugar level increase inhibitor in the fields of food, food additives, cosmetics, medicine, etc., and the polyphenol component of mulberry extract is the same as chlorogenic acid, caffeic acid, astragalin, etc. contained in mulberry leaf extract. It is considered that the substance consists of a group of substances, and these substances have been reported to have an amylase inhibitory action. In particular, chlorogenic acid is found to have a high content in leaf extracts and to be involved in α-amylase inhibitory activity. Chlorogenic acid, which is predicted to have a high content in mulberry strip extract, has been reported to exhibit glucose-6-phosphatase inhibitory action and insulin secretion promoting action involved in the suppression of excessive gluconeogenesis in the liver. In addition to the effects, it can be expected to develop effects such as preventing liver damage and preventing aging.

Claims (2)

Active substance 1-deoxynojirimycin and mulberry extract (mulberry branch) produced from a mulberry strain containing polyphenols, and a soaked extract obtained by immersing mulberry chips that have been dried in the sun after being cut in water, Heated concentrated extract obtained by warm bath. From the results in which the rats in the group administered with 25 ml / kg of the dried mulberry soak extract and 0.5 ml / kg of the heat-concentrated extract suppressed the increase in blood glucose level of the feed group containing starch and sucrose until 100 minutes later, The extract contains 1-deoxynojirimycin and polyphenols according to claim 1, wherein a specific synergistic effect of suppressing glucose absorption in the small intestine is exhibited by inhibition of glucosidase by 1-deoxynojirimycin and amylase inhibition by polyphenols. To extract.