JP2007010565A - Index agent for determining navel odor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an index agent containing a substance suitable as an index for a navel odor, and a determination method for a degree of the navel odor or effectiveness of a deodorant agent, capable of determining objectively and quantitatively the presence of the navel odor, intensity thereof and the effectiveness of the deodorant agent, using the substance as the index. <P>SOLUTION: The present invention provides the index agent for determining the navel odor containing the compound (substance) having a group expressed by a general formula (1):RCOX, the determination method for the navel odor, and the determination method for the effectiveness of the deodorant agent, using the compound as the index, where R represents an alkyl group with a 1-6C, and X represents a divalent or trivalent hetero atom, in the general formula (1). <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an indicator agent that can be used for determination of navel odor, a method for determining the degree of navel odor, or the effectiveness of a deodorant agent.

  In recent years, with an increasing awareness of cleanliness, an increasing number of people are concerned about body odor. Body odor is a collective term for odors that occur from various parts of the body, and the main sites of occurrence are the head (scalp hair odor), oral cavity (mouth odor), axilla (crab odor), inguinal region, sole of the foot (foot odor), etc. There is. A foul odor occurs when the secretions from the apocrine sweat glands developed in the axilla are decomposed by skin resident bacteria, and the foot odor is a high-temperature and high-humidity situation such as in shoes. It is well known that it is decomposed and generated by the action of skin resident bacteria.

  In recent years, clothing that exposes a part of the body has appeared due to a change in fashion, and the width of the body part that is recognized as a deodorant target has been expanded accordingly. In particular, fashion that exposes the abdomen centering on the navel is popular among young women. Under such circumstances, what has become obvious is the navel odor. Therefore, for those who are concerned about navel odor, efforts to reduce the navel odor, such as how much odor originally existed in their navel, or the use of deodorants and cleaning agents, have been successful in the present situation. Whether or not there is an important concern.

  However, when evaluating the degree of body odor and the effectiveness of a deodorant, there is a large room for panelist subjective judgment in sensory evaluation, so quantitative judgment is difficult and objectivity is lacking. Therefore, such an evaluation can be used as a guideline for determining the presence or absence of the body odor constitution, but the degree of odor reduction by the deotorant and the cleaning agent cannot be quantitatively determined. Therefore, a method has been proposed in which the degree of body odor centered on apocrine odor and the effectiveness of a deodorant agent are quantitatively determined using a specific compound as an indicator substance (see, for example, Patent Documents 1 and 2).

  However, there has been no report on the bad smell from the navel, including the main causative substance, and the quantitative judgment has not been realized.

JP 2004-309454 A JP 2005-017272

  An object of the present invention is to objectively and quantitatively determine the presence or absence of a navel odor and the strength or effectiveness of a deodorant using an indicator that contains a substance suitable as an indicator of navel odor and the substance. It is an object of the present invention to provide a method for determining the degree of navel odor or the effectiveness of a deodorant.

  As a result of intensive research on the causative component of the navel odor, the present inventors found that a fatty acid very similar to the navel odor and having a quantifiable concentration of lower fatty acid, particularly isovaleric acid, is present on the inner surface of the navel. Furthermore, it has been found that these components can be used as an objective index for quantitatively determining the degree of odor at the navel.

The present invention provides an indicator for determining navel odor containing a compound having a group represented by the general formula (1).
RCOX (1)
[Wherein, R represents an alkyl group having 1 to 6 carbon atoms, and X represents a divalent or trivalent heteroatom. ]

  Moreover, this invention provides the determination method of the navel smell which uses the said compound as a parameter | index, and the effectiveness determination method of a deodorant agent.

  According to the present invention, it is possible to objectively and quantitatively determine the level of a person's navel odor from the presence or absence and strength of the navel portion.

  In addition, about the latent state of navel odor, such as when there is a large amount of lower fatty acid that is a cause of navel odor in the navel, but it has changed to a weak derivative without salt or other odors In the present invention, it can be detected and accurately evaluated by directly measuring it as a lower fatty acid or a derivative thereof.

  Furthermore, according to the present invention, the effectiveness of a deodorant agent targeting navel odor can be objectively and quantitatively determined.

  The present inventors have analyzed the odorous substance volatilized from the navel by using a device capable of local and selective odor analysis, and a sample wiped from the navel by targeting the navel of a subject where odor is observed in the navel As a result, the main causative substance of navel odor was found to be a lower fatty acid having 2 to 7 carbon atoms such as isovaleric acid (FIGS. 1 and 2). Further, by conducting an odor analysis of the navel portion, it was found that there is a correlation between the abundance of lower fatty acids in the navel portion and the odor intensity (FIG. 3).

[Navel odor indicator]
In the general formula (1), R may be any of a linear or branched alkyl group having 1 to 6 carbon atoms, and the hetero atom represented by X includes an O atom, an N atom, an S atom, and the like. Illustrated. Examples of the compound having a group represented by the general formula (1) include a compound in which X is a hydroxyl group, that is, a fatty acid having a linear or branched alkyl group having 1 to 6 carbon atoms (hereinafter referred to as a lower fatty acid). The Examples of the lower fatty acid include acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, 2-methylbutyric acid, hexanoic acid and the like. Among these, isovaleric acid is present in the umbilical portion. It is a main causative component and is particularly suitable as an indicator.

  Further, the compound having the group represented by the general formula (1) may be a compound obtained by chemically modifying the lower fatty acid using a derivatizing reagent as long as the detection function as an indicator is not lost. . For example, a derivative obtained by converting a carboxy group of a lower fatty acid into a salt, ester, thioester, amide or the like can be used. Specific examples include compounds in which the carboxy group is methyl esterified or trimethylsilylated.

  Derivatives include UV labeling agents such as Op-nitrobenzyl-N, N-diisopropylisourea (PNBDI) and p-bromophenacyl bromide (PBPB), 4-bromomethyl-7-methoxycoumarin (Br-MmC) ) And other fluorescent labeling agents, color developing labeling agents such as 2-nitrophenylhydrazine, silylating agents such as N-trimethylsilylimidazole (TMSI) and N, O-bis (trimethylsilyl) acetamide (BSA), and trifluoroacetic anhydride An acylating agent such as trifluoroacetylimidazole can be used.

  The index agent for determination of navel odor of the present invention can be used alone with the compound itself having the group represented by the general formula (1), and water, methanol, ethanol, ether, 2-propanol in order to enhance its handling property In addition, a solvent for dissolving or diluting, such as dichloromethane, can be contained, and other stabilizers, antiperspirants, fungicides, antibacterial agents, surfactants, antioxidants, perfumes, plants Additives such as extracts can be blended to make a composition suitable for practical use such as storage and use in judgment tests.

  The index agent for determining navel odor of the present invention preferably contains 0.0001 to 100% by mass, more preferably 0.01 to 100% by mass of the compound having a group represented by the general formula (1) in the index agent. it can.

[Method of judging navel odor]
Lower fatty acids are suitable as an index because they can be easily quantified by themselves and can be easily quantified as a derivative obtained by chemically modifying a carboxy group. That is, by measuring the abundance of the lower fatty acid or its derivative in the navel part by an appropriate method chemically or physically, the odor of the navel part can be objectively and quantitatively determined.

  A method for collecting a sample from the navel portion is not particularly limited, and for example, a device described in Japanese Patent Laid-Open No. 2003-21582 that can selectively perform odor analysis on a local area can be used. Specifically, a probe made of Teflon (trademark of DuPont) is inserted into the navel recess, and the surface of the navel recess is purged directly. The purged air is concentrated in the adsorption tube, and the adsorption tube is subjected to gas chromatography equipped with a dedicated heat desorption device, whereby data proportional to the volatilization amount of the odorous substance can be obtained. Alternatively, the sample can be collected by wiping from the navel with a degreasing gauze impregnated with water or the like.

  The amount of the lower fatty acid or derivative thereof in the collected sample can be measured, for example, by gas chromatography. For example, when the content of isovaleric acid present in the navel is measured by GC-MS. Prepares a calibration curve using isovaleric acid or its derivative as a standard substance (standard). What is necessary is just to identify the peak of isovaleric acid contained in the sample extract | collected using this analytical curve. The measurement of lower fatty acids or derivatives thereof can also be performed by optically measuring the sample by subjecting the lower fatty acids in the sample to ultraviolet labeling, fluorescence labeling, etc., using the derivatization reagent described above. . In this case, a calibration curve is prepared in advance using the labeling agent of the present invention containing a lower fatty acid or a derivative thereof as a standard substance, and the lower fatty acid contained in the collected sample is measured by using this calibration curve. In addition, the degree of navel odor can be determined.

  The determination of navel odor can also be performed by sensory evaluation. When sensory evaluation is carried out, an odor standard sample of each concentration is prepared using an index of the present invention in which fatty acid or a derivative thereof, preferably isovaleric acid or a derivative thereof as an active ingredient is diluted in several stages. And the odor of the subject sample which prepared the odor of the sample extract | collected from the navel part is collated with a standard sample, and what is necessary is just to determine the quantity of the lower fatty acid or its derivative contained in a sample by sensory evaluation. In addition, sensory evaluation includes, for example, evaluation for directly judging the color, evaluation using a test paper impregnated with an indicator agent, and the like.

  In addition, when a lower fatty acid is labeled with a chromophore in the visible region such as 2-nitrophenylhydrazine as a derivatizing reagent to give carboxylic acid 2-nitrophenylhydrazide, the concentration of the lower fatty acid derivative-color development It is also possible to prepare a standard sample and use it for sensory evaluation.

  In this way, the degree of navel odor can be objectively and quantitatively measured by observing color tone and measuring using an analytical instrument, in addition to olfaction.

  In addition, since the amount of the lower fatty acid in the navel portion coincides with the presence and intensity of the navel odor, the quantitative analysis of the lower fatty acid indicates whether the subject has a possibility of generating a navel odor, that is, potential. Evaluation can be made.

  In addition, when there is a large amount of lower fatty acid in the navel, but it has no odor such as salt or is changed to a weak derivative, there is a latent state of navel odor. However, such a state cannot be evaluated accurately even if a sensory evaluation or a fact-finding with a high correlation with navel odor is conducted. In contrast, in the present invention, the latent state of navel odor can be detected and accurately evaluated by directly measuring such a derivative.

[Method of judging effectiveness of deodorant]
In the present invention, the effectiveness of the deodorant agent targeting navel odor can be objectively and quantitatively determined using an indicator agent containing a lower fatty acid or a derivative thereof.

  In determining the effectiveness of the deodorant agent, the lower fatty acid or a derivative thereof may be used alone as an indicator of the present invention, and other components such as a solvent or stabilizer for dissolution or dilution, Additives such as antiperspirants, bactericides, antibacterial agents, surfactants, antioxidants, fragrances, plant extracts, etc., and use them as compositions that are practical for storage and use in judgment tests, etc. Also good.

  Deodorant agents to be evaluated for effectiveness are (1) a type that sterilizes bacteria on the skin and prevents the degradation of skin waste products, (2) a type that decomposes or changes odor components into odorless derivatives, (3 ) It may be due to any type of mechanism of action, such as a type that masks odors. The method of using a lower fatty acid or a derivative thereof as an indicator for determining the effectiveness of a deodorant is not particularly limited, and may be used according to the action mechanism and evaluation method of the deodorant.

  For example, by adding a predetermined amount of a deodorant agent sample to an indicator agent containing a lower fatty acid or a derivative thereof, preferably isovaleric acid or a derivative thereof at a predetermined concentration, and quantifying the change state of the indicator agent by an appropriate method. The effectiveness of the deodorant sample can be determined objectively and quantitatively.

  As a method for quantifying the state of change of the indicator, when the deodorant sample is a type that reduces the odor by degrading or inducing the isovaleric acid of (2) above to another compound, calibration of the indicator is performed. A line may be prepared in advance, and an instrumental analysis may be performed using this calibration curve, or a changed or unchanged substance of the indicator agent may be quantified by chemical analysis such as titration or extraction. When the deodorant sample is the type masking the navel odor of (3) above, prepare the odor standard sample of each concentration by diluting the indicator agent in several stages, and add the deodorant agent sample. The odor is checked against a standard sample, and the masking effect may be determined by sensory evaluation.

  In addition, for example, a predetermined amount of a deodorant agent sample is added to an indicator containing a fluorescently labeled compound of lower fatty acid at a predetermined concentration, and the change state of the labeled compound is subjected to instrumental analysis using a calibration curve of the same labeled compound. You may quantify with. In addition, when a predetermined amount of a deodorant agent sample is added to an indicator containing a lower fatty acid at a predetermined concentration, the labeling moiety is used when the lower fatty acid change or unchanged substance is quantified by chemical analysis such as titration or extraction. You may detect using.

  Furthermore, a sample of a deodorant agent is actually applied to a human navel, and samples such as navel sweat collected before and after application are evaluated using lower fatty acids or derivatives thereof as an index, and compared, so-called in In vivo evaluation can be performed.

  In this way, the effectiveness of the deodorant can be determined objectively and quantitatively by observing the color tone by visual observation or measurement using an analytical instrument in addition to the sense of smell.

Test example 1
28 healthy Japanese subjects were randomly selected. A sensory evaluation was conducted by seven specialist panelists on the odor of the navel. The evaluation criteria were four levels of “strongly smelling”, “smelling”, “slightly smelling”, and “not smelling”, and the judgment was made based on the agreement of seven people. As a result, among the subjects, there were 6 strongly smelling people, 6 smelling people, 2 slightly smelling people, and 14 not smelling people.

Example 1
Among the 14 people who had odors in Test Example 1, from a subject having a typical navel odor, a device described in Japanese Patent Application Laid-Open No. 2003-21582 that can selectively analyze odors locally is used. The navel was analyzed for odor. A probe made of Teflon (trademark of DuPont) was inserted into the subject's navel, and the odor component of the navel was collected in an adsorption tube at a purge flow rate of 90 mL / min and a suction rate of 80 mL / min for 10 minutes. The adsorption tube was introduced into a gas chromatography-mass spectrometer (Agilent Technology, GC-MS system) equipped with a heat desorption apparatus (Gestel), and the amount of odor components was calibrated. The measurement conditions are as follows.
Column: J & W, DB-1 (60m x ID 0.25mm)
Oven: Initial temperature 40 ℃
Temperature rise 2 ℃ / min, 300 ℃

  The results of GC-MS analysis are shown in FIG. From this result, the presence of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, 2-methylbutyric acid, valeric acid, and hexanoic acid as lower fatty acids is shown in the navel dents of people with a navel odor, and the peak From the area, it was found that these lower fatty acids were present at high concentrations. This eluted component had a strong odor very similar to navel odor in odor sniffing gas chromatography. From this, it was confirmed that the lower fatty acid is suitable as a determination index agent for navel odor.

Example 2
Of the 14 people who had odors in Test Example 1, a subject having a typical navel odor wiped off the navel using a degreased gauze impregnated with 1 mL of purified water, and then 1N water from the wiped gauze. Extraction was performed using 10 mL of an aqueous sodium oxide solution, and after neutralization, the acidic fraction was concentrated with purified diethyl ether to prepare an extraction sample. The sample was introduced into the same gas chromatography-mass spectrometer (Agilent Technology, GC-MS system) as used in Example 1, and it was confirmed that the lower fatty acid was mainly (FIG. 2). The measurement conditions are as follows.
Column: J & W, DB-1 (60m x ID 0.25mm)
Oven: Initial temperature 60 ℃
Temperature rise 2 ℃ / min, 300 ℃

Example 3
Isovaleric acid was quantified using GC-MS according to the same procedure as in Example 1, targeting a total of 28 subjects in the same group as in Test Example 1. From these results, a group of subjects who strongly smell navel odor (6 people), a group of subjects who smell navel odor (6 people), a group of subjects who smell slightly navel odor (2 people), and a group of subjects who do not smell navel odor (14 people) The average detection amount for each group was calculated. As shown in FIG. 3, there is a correlation between the detected amount and the odor intensity, and the stronger the navel odor, the more the amount of isovaleric acid in the navel part, while in subjects without odor, Fatty acids were below the detection limit, especially isovaleric acid was not detected. From this, it was confirmed that isovaleric acid was suitable as a determination index agent for navel odor.

It is an elution peak showing the result of GC-MS analysis of components collected by purging the navel surface of a person with navel odor.

It is the elution peak which shows the result of having analyzed the extraction component extract | collected by gauze the navel surface of a person with a navel smell.

It is a graph which shows the relationship between the detection amount of the isovaleric acid of the component extract | collected by purging the navel surface of the person with and without a navel smell, and the intensity of odor.

Claims (6)

An index agent for determining navel odor containing a compound having a group represented by the general formula (1).
RCOX (1)
[Wherein, R represents an alkyl group having 1 to 6 carbon atoms, and X represents a divalent or trivalent heteroatom. ]   The index agent for determining navel odor according to claim 1, wherein the compound having a group represented by the general formula (1) is a fatty acid.   The indicator for navel odor determination according to claim 1 or 2, wherein R in the general formula (1) is an alkyl group having 4 or 5 carbon atoms.   The indicator agent for determining navel odor according to claim 3, wherein the compound having a group represented by the general formula (1) is isovaleric acid. A method for determining navel odor, using the compound having the group represented by the general formula (1) as an index.
RCOX (1)
[Wherein, R represents an alkyl group having 1 to 6 carbon atoms, and X represents a divalent or trivalent heteroatom. ] A method for determining the effectiveness of a deodorant agent using a compound having a group represented by the general formula (1) as an index.
RCOX (1)
[Wherein, R represents an alkyl group having 1 to 6 carbon atoms, and X represents a divalent or trivalent heteroatom. ]