JP2006333827A - UTILIZATION OF CHROMOSOME SNPs ALLELE FOR IDENTIFYING BREWING YEAST - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for discriminating the strains of yeast from each other, a method for classifying the strains of the yeast and a method for selecting the strains of the yeast having characteristic brewing properties. <P>SOLUTION: It is possible to identify and classify the strains of the yeast, and select the strains having the characteristic brewing properties by preparing primer sets for gene polymorphisms and detecting the genome DNA of the yeast by using ≥1 polymorphism as indices. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a yeast strain identification method, a classification method, and a strain selection method having desired brewing characteristics.

  Conventionally, the identification of yeast has mainly relied on biochemical tests such as a sugar assimilation test by culture. These tests take time, and the test results are not always reliable due to the influence of culture conditions.

  Yeasts used for beer brewing are roughly classified into two types. They are a top fermentation yeast used for top fermentation and a bottom fermentation yeast used for bottom fermentation.

  The top fermenting yeast is classified as Saccharomyces cerevisiae, while the bottom fermenting yeast is classified as Saccharomyces pastorianus. Saccharomyces pastorianus is known to be a heteroploid having both two types of genomes: Saccharomyces cerevisiae and its related species, Saccharomyces bayanus. As the two subgenomes constituting the genome of Saccharomyces pastorianus, those derived from Saccharomyces cerevisiae are referred to as Sc-type genomes, and those derived from Saccharomyces bayanus are referred to as Lg-type genomes. In this way, the two types of strains, top fermented yeast and bottom fermented yeast, are very closely related and both are used for brewing beer. It is difficult to distinguish strains by methods. Furthermore, identification of strains within the same species is even more difficult.

  Examples of strain identification methods that replace biochemical tests include methods for detecting differences in DNA levels. JP-A-9-299090 “Primer used for detection of S. diastaticus” describes a method for detecting Saccharomyces diastaticus, a harmful wild yeast on beer brewing, and JP-A-11-56366 “Method for identifying yeast” A method for distinguishing and identifying brewing yeast and non-brewing yeast is described, and Japanese Patent Application Laid-Open No. 2002-233382 “Determination of beer yeast” describes a method for discriminating bottom fermentation yeast for brewing from wild yeast. These methods are all methods for discriminating brewing yeasts from other yeasts, and are not methods for discriminating between brewing yeast strains. That is, these methods could not identify yeast strains of the same use that are genetically very close.

  As a method for identifying a yeast strain by detecting a difference in DNA level, JP 2005-27527 “Strain yeast strain identification method” describes a method for identifying a strain belonging to sake yeast. However, there has never been a method for discriminating strains for brewing yeast other than sake yeast.

  Since SNP (Single Nucleotide Polymorphism, single nucleotide polymorphism) can discriminate slight differences in DNA level, it is considered that it can be used to identify genetically very close varieties. JP 2002-209581 “Cow breed identification method” describes a cow breed identification method using SNP. However, there is still no method for yeast strain identification using SNP.

  As in the case of yeast identification, the classification of yeast has mainly depended on biochemical tests such as a sugar assimilation test by culture. These tests take time, and the test results are not always reliable due to the influence of culture conditions.

  Examples of strain identification methods that replace biochemical tests include methods for detecting differences in DNA levels. Although there is a literature that attempted classification by the AFLP (Amplified Fragment Length Polymorphism) method (see, for example, Non-Patent Document 1), it was insufficient for classification of yeast strains of genetically very similar uses.

  Sulfurous acid, esters, and higher alcohols are all important aroma components that greatly affect the flavor of alcoholic beverages.

  Sulfurous acid is a substance having a high antioxidant effect, and is widely used as an antioxidant in the fields of food, beverages, pharmaceuticals and the like. Also in beer brewing, it is known that the quality retention period will be prolonged depending on the increase in the concentration of sulfite contained in the product, and if the concentration of sulfite in the product is increased, the flavor stability is excellent and the quality is maintained for a long time. A product with a period can be manufactured.

  Numerous attempts have been made to increase the ability of yeast to produce sulfite using genetic recombination techniques (see, for example, Patent Document 6, Non-patent Document 2, and Non-patent Document 3). In view of the above image and the like, it is not preferable to increase the ability of yeast to produce sulfite using genetic recombination technology.

  Hydrogen sulfide is a substance having a “rotted egg” -like odor, and in beer brewing, it not only causes off-flavor of the product but also a precursor of other off-flavor causing substances. Therefore, it is desirable that the ability of yeast to produce hydrogen sulfide is low.

  Attempts have also been made to reduce the ability of yeast to produce hydrogen sulfide using genetic recombination techniques. Japanese Patent No. 2005398 “Yeast Hydrogen Sulfide Production Suppressing Gene and Brewing Yeast Introduced with the Gene” describes a method for reducing the hydrogen sulfide production ability of yeast by introducing NHS5 gene. Japanese Patent No. 3514507 “Hydrogen Sulfide Production” "Yeast with reduced ability and beer production method using this yeast" describes a method for reducing the hydrogen sulfide producing ability of yeast by constitutively expressing the MET25 gene. However, considering the consumer image of genetically modified foods, it is not preferable to reduce the ability of yeast to produce hydrogen sulfide using genetic modification techniques.

  It is common to select a strain having a high sulfite-producing ability or a strain having a low hydrogen sulfide-producing ability from many yeast strains. However, in order to select these strains, it was necessary to conduct a fermentation test. The fermentation test takes time, and the test results are not always reliable due to the influence of culture conditions.

  Esters are particularly important aroma components that determine the flavor characteristics of liquors and are substances produced during fermentation by yeast. Higher alcohols are also substances produced by yeasts, but higher alcohols not only affect the flavor of alcoholic beverages, but also become precursors of ester production, which is an important aroma component, and the content of alcoholic beverages in the product This is an important factor directly related to the characteristics of Conventionally, it was necessary to conduct a fermentation test in order to select yeast strains with high or low ester or higher alcohol production ability. The fermentation test takes time, and the test results are not always reliable due to the influence of culture conditions.

JP-A-9-299090 JP-A-11-56366 JP 2002-233382 A Japanese Patent Laying-Open No. 2005-27527 JP 2002-209581 A JP 2004-283169 A Japanese Patent No. 2005398 Japanese Patent No. 3514507 M.M. Azumi et al. “Yeast”, Vol. 18, p. 1145 to 1154, 2001 C. Korch et al. "Proc. Eur. Brew. Conv. Congress., Lisbon", p. 201-208, 1991 J. et al. Hansen et al. "Nature Biotech.", Vol. 14, p. 1587-1591, 1996

  In brewing beer, happoshu and other miscellaneous sake, yeast not only performs alcoholic fermentation but also produces various aroma components. Depending on the combination of yeast strains, wort components and fermentation control methods, the flavor of beer, happoshu and other miscellaneous sakes will be characterized, so classification and identification of yeast strains will ensure proper brewing management. This is an essential technology. As described above, establishment of a technique for accurately identifying and classifying a large number of yeast strains in a short time in an actual liquor production site and in a test research institution has been a serious problem. Sulfurous acid is an important substance that affects the quality retention period of beer, happoshu, and other miscellaneous sake, and esters and higher alcohols are used in beer, happoshu, and other miscellaneous sakes in terms of product flavor. It is an important aroma component that determines its characteristics. The present invention relates to a method for distinguishing yeast strains for brewing from each other, a method for classifying yeast strains for brewing, and yeast having high or low ability to produce substances such as sulfite, hydrogen sulfide, esters, and higher alcohols. It was made for the purpose of providing a method for selecting the.

  As a result of intensive studies to solve the above problems, the present inventors have detected alleles for gene polymorphisms at 33 loci searched genome-wide for genomic DNA of yeast, and combinations of the detected alleles Based on the above, the yeast strains to which the genomic DNA belongs were identified, and the yeast strains were distinguished from each other and the yeasts were classified. Furthermore, by examining specific alleles, we succeeded in selecting yeasts with high or low ability to produce substances such as sulfurous acid, hydrogen sulfide, esters, and higher alcohols. The present invention has been completed based on this result.

That is, the present invention is as follows.
For the genomic DNA of brewer's yeast, alleles consisting of a combination selected from the following (1) to (33) or a plurality selected from (1) to (33) are detected, and the yeast strain is determined based on the result. Yeast strain identification method and classification method characterized by:
(1) At least one of (a) to (ki) of SEQ ID NO: 1:
(A) an allele which is a base C or T present at the position of base number 53,
(A) an allele that is base A or G present at the position of base number 140;
(C) an allele whose base C or T is at the position of base number 170,
(D) an allele that is base A or G present at the position of base number 185,
(E) an allele that is base A or G present at the position of base number 225,
(F) an allele which is the base G or T present at the position of the base number 239, and (g) an allele which is the base A or G present at the position of the base number 298;

(2) at least one of (a) to (e) of SEQ ID NO: 2:
(A) an allele which is the base C or T present at the position of the base number 104;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base C or T present at the position of base number 304,
(D) an allele which is the base C or T present at the position of the base number 321 and (e) an allele which is the base A or G present at the position of the base number 322;

(3) at least one of (a) to (c) of SEQ ID NO: 3:
(A) an allele which is a base C or T present at the position of base number 125,
(A) an allele that is base A or G present at the position of base number 145;
(C) an allele with insertion or non-insertion of 3 bases present at positions of base numbers 351 to 354;

(4) at least one of (a) to (co) of SEQ ID NO: 4:
(A) an allele which is a base C or T present at the position of base number 67,
(A) an allele which is a base C or T present at the position of base number 74;
(C) an allele that is base A or G present at the position of base number 104;
(D) an allele which is a base C or T present at the position of base number 160;
(E) an allele that is base A or C present at the position of base number 268,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base A or G present at the position of base number 324;
(H) an allele that is base C or T present at the position of base number 446;
(G) an allele which is the base G or T present at the position of the base number 453, and (co) an allele which is the base A or G present at the position of the base number 466;

(5) At least one of (a) and (b) of SEQ ID NO: 5:
(A) an allele which is the base A or G present at the position of the base number 431, and (b) an allele which is the base C or T present at the position of the base number 464;

(6) At least one of (A) to (B) of SEQ ID NO: 6:
(A) an allele that is base A or C present at the position of base number 75;
(A) an allele which is the base C or T present at the position of the base number 185,
(C) an allele which is the base C or T present at the position of the base number 216,
(D) an allele that is base A or C present at the position of base number 217,
(E) an allele that is base A or C present at the position of base number 253,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base C or T present at the position of base number 318,
(H) an allele that is base A or T present at the position of base number 441;
(G) an allele that is base A or G present at the position of base number 444;
(E) an allele that is base A or T present at the position of base number 445;
(Sa) an allele that is a base C or T present at the position of base number 463, and (f) an allele that is a base C or T present at the position of base number 493;

(7) At least one of (a) to (f) of SEQ ID NO: 7:
(A) an allele that is base A or G present at the position of base number 213,
(A) an allele that is base A or G present at the position of base number 243;
(C) an allele that is base C or T present at the position of base number 290,
(D) an allele that is base A or T present at the position of base number 348;
(E) an allele which is the base A or G present at the position of the base number 350, and (f) an allele which is the base C or T present at the position of the base number 378;

(8) at least one of (A) to (E) of SEQ ID NO: 8:
(A) an allele that is base A or G present at the position of base number 88;
(A) an allele which is a base C or T present at the position of base number 152;
(C) an allele that is base C or T present at the position of base number 161;
(D) an allele which is the base A or G present at the position of the base number 309, and (e) an allele which is the base C or T present at the position of the base number 381;

(9) At least one of (A) to (U) of SEQ ID NO: 9:
(A) an allele which is a base C or T present at the position of base number 66;
(A) an allele that is the base C or T present at the position of the base number 229, and (c) an allele that is the base A or T at the position of the base number 262;

(10) At least one of (a) to (c) of SEQ ID NO: 10:
(A) an allele that is a deletion or non-deletion of 1 base T present at positions from base numbers 163 to 170;
(A) an allele that is deleted or non-deletion of 1 base A present at the position of base numbers 309 to 317, and (c) an allele that is base A or C at the position of base number 356;

(11) At least one of (a) to (ki) of SEQ ID NO: 11:
(A) an allele that is base A or T present at the position of base number 69;
(A) an allele which is a base C or T present at the position of base number 93;
(C) an allele that is base A or G present at the position of base number 111;
(D) an allele which is the base C or T present at the position of the base number 208;
(E) an allele that is base A or G present at the position of base number 210;
(F) an allele which is the base C or T present at the position of the base number 408, and (g) an allele which is the base A or G present at the position of the base number 429;

(12) At least one of (a) to (c) of SEQ ID NO: 12:
(A) an allele that is base A or G present at the position of base number 104;
(A) an allele that is the base C or G present at the position of the base number 151, and (c) an allele that is the base C or T present at the position of the base number 343;

(13) at least one of (a) to (c) of SEQ ID NO: 13:
(A) an allele that is base A or G present at the position of base number 126;
(A) an allele that is the base C or G present at the position of the base number 201, and (c) an allele that is the base A or G present at the position of the base number 264;

(14) At least one of (A) to (U) of SEQ ID NO: 14:
(A) an allele that is base A or C present at the position of base number 132;
(A) an allele that is base A or C present at the position of base number 185, and (c) an allele that is base C or T present at the position of base number 189;

(15) at least one of (A) to (E) of SEQ ID NO: 15:
(A) an allele that is base C or T present at the position of base number 101,
(A) an allele that is base C or T present at the position of base number 183,
(C) an allele that is inserted or non-inserted into 1 base A existing at positions of base numbers 188 to 192,
(D) an allele which is the base C or T present at the position of the base number 280, and (e) an allele which is the base G or T present at the position of the base number 338;

(16) At least one of (a) to (sa) of SEQ ID NO: 16:
(A) an allele that is base A or T present at the position of base number 134;
(A) an allele that is base A or T present at the position of base number 135;
(C) an allele that is base C or T present at the position of base number 137,
(D) an allele that is base A or T present at the position of base number 138;
(E) an allele that is base A or G present at the position of base number 142;
(F) an allele that is base A or G present at the position of base number 165,
(G) an allele that is base C or T present at the position of base number 183,
(H) an allele that is base A or C present at the position of base number 219,
(G) an allele that is base C or G present at the position of base number 232;
(Co) an allele that is base A or G at the position of base number 293, and (sa) an allele that is base C or T at the position of base number 332;

(17) at least one of (a) to (e) of SEQ ID NO: 17:
(A) an allele that is base A or T present at the position of base number 65,
(A) an allele that is base A or G present at the position of base number 158,
(C) an allele that is the base G or T at the position of base number 236,
(D) an allele that is base A or G present at the position of base number 256, and (e) an allele that is base C or T at the position of base number 474;

(18) at least one of (a) and (b) of SEQ ID NO: 18:
(A) an allele which is the base C or T present at the position of the base number 96, and (a) an allele which is the base C or T present at the position of the base number 133;

(19) At least one of (a) to (e) of SEQ ID NO: 19:
(A) an allele that is base A or G present at the position of base number 261,
(A) an allele that is base C or T present at the position of base number 282,
(C) an allele that is base C or T present at the position of base number 371, and (d) an allele that is base C or T at the position of base number 440;

(20) At least one of (a) and (b) of SEQ ID NO: 20:
(A) an allele which is the base A or G present at the position of the base number 247, and (b) an allele which is the base C or T present at the position of the base number 362;

(21) at least one of (a) to (e) of SEQ ID NO: 21:
(A) an allele that is base A or G present at the position of base number 82;
(A) an allele that is base A or G present at the position of base number 91;
(C) an allele that is the base G or T present at the position of the base number 139, and (d) an allele that is the base C or T at the position of the base number 140;

(22) (a) an allele that is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) at least one of (a) and (b) of SEQ ID NO: 23:
(A) an allele which is the base A or T present at the position of the base number 420, and (b) an allele which is the base C or G present at the position of the base number 485;

(24) at least one of (a) to (co) of SEQ ID NO: 24:
(A) an allele which is the base C or T present at the position of base number 189,
(A) an allele that is base A or G present at the position of base number 209;
(C) an allele that is base C or T present at the position of base number 218,
(D) an allele that is base C or T present at the position of base number 260;
(E) an allele that is base C or T present at the position of base number 283,
(F) an allele that is base C or G present at the position of base number 315,
(G) an allele that is base A or G present at the position of base number 379;
(H) an allele which is the base C or T present at the position of the base number 385,
(G) an allele which is the base A or C present at the position of the base number 551, and (co) an allele which is the base C or T present at the position of the base number 597;

(25) at least one of (a) to (ki) of SEQ ID NO: 25:
(A) an allele which is a base C or T present at the position of base number 62;
(A) an allele that is base A or G present at the position of base number 96;
(C) an allele that is base A or G present at the position of base number 171;
(D) an allele that is base C or T present at the position of base number 176,
(E) an allele that is base C or T present at the position of base number 222;
(F) an allele which is the base C or T present at the position of the base number 228, and (g) an allele which is the base A or G present at the position of the base number 273;

(26) At least one of (a) to (ki) of SEQ ID NO: 26:
(A) an allele that is base A or G present at the position of base number 57;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base A or T present at the position of base number 221;
(D) an allele that is base A or G present at the position of base number 303;
(E) an allele that is base A or T present at the position of base number 350;
(F) an allele that is base A or T present at the position of base number 400, and (g) an allele that is base A or G that is present at the position of base number 434;

(27) At least one of (a) to (e) in SEQ ID NO: 27:
(A) an allele that is base A or G present at the position of base number 80;
(A) an allele that is a deletion or non-deletion of base A present at positions from base numbers 116 to 122;
(C) an allele that is base A or G present at the position of base number 204;
(D) an allele that is base C or T present at the position of base number 249, and (e) an allele that is base G or T at the position of base number 389;

(28) At least one of (a) to (e) of SEQ ID NO: 28:
(A) an allele that is base A or C present at the position of base number 83;
(A) an allele which is a base C or G present at the position of base number 169,
(C) an allele that is base C or T present at the position of base number 193,
(D) an allele that is the base A or C existing at the position of the base number 215, and (e) an allele that is the base A or G that exists at the position of the base number 232;

(29) (a) an allele that is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) an allele which is the base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) An allele of base C or T present in the position of base number 162 of SEQ ID NO: 31;
(32) At least one of (a) and (b) of SEQ ID NO: 32:
(A) an allele that is the base C or T present at the position of the base number 34, and (a) an allele that is the base A or G that is present at the position of the base number 336;

(33) (a) An allele of base A or G present in the position of base number 361 of SEQ ID NO: 33;
The allele consisting of any combination of (1) and (2) and (3) below is detected for the genomic DNA of yeast, and based on the results, yeast having a high ability to produce sulfite and hydrogen sulfide producing ability A method for selecting at least one of low yeast, yeast having high ester-producing ability, yeast having low ester-producing ability, yeast having high ability to produce higher alcohol, and yeast having low ability to produce higher alcohol:
(1) at least one of SEQ ID NO: 17 from (a) to (e):
(A) an allele that is base A or T present at the position of base number 65,
(A) an allele that is base A or G present at the position of base number 158,
(C) an allele that is the base G or T at the position of base number 236,
(D) an allele that is base A or G present at the position of base number 256, and (e) an allele that is base C or T at the position of base number 474;
(2) at least one of (A) to (E) of SEQ ID NO: 1
(A) an allele which is a base C or T present at the position of SEQ ID NO: 53,
(A) an allele having a base C or T at the position of SEQ ID NO: 170,
(C) an allele which is the base A or G present at the position of SEQ ID NO: 185,
(D) an allele that is the base G or T at the position of SEQ ID NO: 239, and (e) an allele that is the base A or G at the position of SEQ ID NO: 298;
(3) at least one of (a) and (b) of SEQ ID NO: 2
(A) an allele which is the base C or T present at the position of the base number 104, and (a) an allele which is the base C or T present at the position of the base number 321;
In the above method, it is desirable that the yeast strain is a top fermentation yeast or a bottom fermentation yeast.

  In the above method, the direct sequencing method, the DHPLC (Determining High Performance Liquid Chromatography) method, the method using Surveyor Nuclease, the TaqMan PCR method, the Invader method, the primer extension method, the SSCP (Simple Mon Stroke Method). The detection can be performed using one or two or more methods selected from the group consisting of a method using a DNA chip.

  Furthermore, the present invention is a kit containing a primer, oligonucleotide, primer or oligonucleotide used for the detection of the allele.

  The present invention makes it possible to perform classification, identification and identification of yeast quickly, simply and with high accuracy. According to the present invention, it is possible to identify strains quickly and with high accuracy, and in producing beer, happoshu, and other miscellaneous sakes, it is possible to produce products that stably exhibit flavor characteristics. Is played.

  In addition, according to the present invention, not only distinction between different brewing yeasts such as top fermentation yeast and bottom fermentation yeast, but also discrimination between identical brewing yeasts that are very difficult and delicate, for example, strains between the same bottom fermentation yeasts, In the liquor manufacturing industry, test research institutes, etc., the present invention enables quick, simple and highly accurate classification, identification and identification of yeast. Clear discrimination is also possible.

  Furthermore, according to the present invention, it is possible to efficiently select yeast strains with high or low ability to produce sulfurous acid, hydrogen sulfide, esters, and higher alcohols without conducting complicated and inaccurate fermentation tests.

  Embodiments of the present invention will be described below. This identification / classification / selection method is a method for discriminating an allele type in a yeast gene sample and identifying / classifying / selecting a yeast strain from the allele type.

  In the present invention, allele detection or determination or allele type detection or determination refers to detection and determination of an allele having a single nucleotide polymorphism in the genomic DNA of a yeast strain to be identified, classified and selected. In this case, detection is performed for one chromosome on a pair of chromosomes as well as detection for both chromosomes. When detecting for both chromosomes, homozygosity or heterozygosity at a single nucleotide polymorphism site is included. Including detection. In addition, allele detection or allele type detection also means detection and determination of a base at a single nucleotide polymorphism site in a yeast strain.

  The gene sample preferably means a gene (specifically, DNA) of a cell of top fermentation yeast or bottom fermentation yeast, or a sample containing this as a main component. Preparation of a gene sample from yeast cells can be performed according to a generally known DNA extraction / purification method. It is also possible to prepare a gene sample using a commercially available DNA extraction / purification kit.

  In this identification method, the allele type of yeast can be determined using the SNP of the region containing the sequences shown in SEQ ID NOS: 1 to 33 as an index. Specifically, the discrimination of the allele type can be performed based on information represented by any of the following (1) to (33).

(1) The nucleic acid contained in the gene sample contains any one or a plurality of bases of the 53rd, 140th, 170th, 185th, 225th, 239th, 298th or more of the base sequence represented by SEQ ID NO: 1. Information about which allele type of the nucleotide sequence shown in Table 2 is the nucleic acid having the nucleotide sequence to be included (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 1).

(2) The nucleic acid contained in the gene sample is a nucleotide sequence containing any one or a plurality of bases of the 104th, 149th, 304th, 321st, 322th of the base sequence represented by SEQ ID NO: 2. Information on which nucleic acid possesses the base sequence of which allele type shown in 3 (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 2).

(3) The nucleic acid contained in the gene sample is shown in Table 4 that includes one or more of the 125th, 145th, 351st to 354th bases of the base sequence represented by SEQ ID NO: 3. Information about which nucleic acid possesses the base sequence of which allele type (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 3).

(4) The nucleic acid contained in the gene sample is the 67 th, 74 th, 104 th, 160 th, 268 th, 269 th, 324 th, 446 th, 453 th, 466 among the base sequence represented by SEQ ID NO: 4 Information about which nucleotide sequence of any of the allele types shown in Table 5 is included in the base sequence containing any or a plurality of bases (allele type relating to the region containing the sequence shown in SEQ ID NO: 4) Discriminating information).

(5) The nucleic acid contained in the gene sample is any of the allele types shown in Table 6 in which the base sequence including any one of the 431rd and 464th bases or a plurality of bases among the base sequence shown in SEQ ID NO: 5 Information about whether the nucleic acid has a base sequence (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 5).

(6) The nucleic acid contained in the gene sample is the 75th, 185th, 216th, 217th, 253rd, 269th, 318th, 441th, 444th, 444th, 445 of the nucleotide sequence represented by SEQ ID NO: 6 Information about which nucleotide sequence of any of the allele types shown in Table 7 is included in the base sequence containing any of the 1st, 463rd, 493th or multiple bases (sequence shown in SEQ ID NO: 6) Allele type discrimination information for the area that contains).

(7) A nucleotide sequence in which the nucleic acid contained in the gene sample contains any one or a plurality of bases of the 213rd, 243rd, 290th, 348th, 350th, 378th of the base sequence represented by SEQ ID NO: 7 Is information about which of the allele types shown in Table 8 is a nucleic acid (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 7).

(8) The nucleic acid contained in the gene sample is a base sequence containing any one or a plurality of bases of 88th, 152th, 161st, 309th, 381 among the base sequences represented by SEQ ID NO: 8 Information about which nucleic acid possesses the base sequence of which allele type shown in 9 (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 8).

(9) Among the nucleotide sequences represented by SEQ ID NO: 9, the nucleic acid contained in the gene sample is any of the nucleotide sequences including any of the 66th, 229th, 262th or a plurality of bases shown in Table 10. Information about whether the nucleic acid has an allele-type base sequence (allele-type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 9).

(10) The nucleic acid contained in the gene sample is a nucleotide sequence containing any one or a plurality of bases from the 163rd to the 170th, the 309th to the 317th, the 356th, among the base sequences represented by SEQ ID NO: 10. Information on which allele type of nucleotide sequence shown in No. 11 is a nucleic acid (allele type discrimination information on the region containing the sequence shown in SEQ ID NO: 10).

(11) The nucleic acid contained in the gene sample contains any one or a plurality of bases of the 69th, 93rd, 111th, 208th, 210th, 408th, 429th of the base sequence represented by SEQ ID NO: 11 Information about which allele type of the nucleotide sequence shown in Table 12 is the nucleic acid having the nucleotide sequence to be included (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 11).

(12) The nucleic acid contained in the gene sample is any one of the nucleotide sequences represented by SEQ ID NO: 12 and any one of the nucleotide sequences including any of the 104th, 151st, 343rd or multiple bases shown in Table 13 Information about whether or not the nucleic acid has an allele-type base sequence (allele-type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 12).

(13) The nucleic acid contained in the gene sample is any one of the nucleotide sequences represented by SEQ ID NO: 13 that includes any of the 126th, 201st, 264th or multiple nucleotide sequences shown in Table 14. Information about whether or not the nucleic acid has an allele-type base sequence (allele-type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 13).

(14) The nucleic acid contained in the gene sample is any one of the base sequences represented by SEQ ID NO: 14, including any of the 132rd, 185th, and 189th base sequences or a plurality of base sequences shown in Table 15. Information about whether the nucleic acid has an allele-type base sequence (allele-type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 14).

(15) The nucleic acid contained in the gene sample is a nucleotide sequence comprising any one of the 101st, 183rd, 188 to 192nd, 280th, 338th or a plurality of bases in the nucleotide sequence represented by SEQ ID NO: 15 Information on which allele type of the nucleic acid sequence shown in Table 16 is a nucleic acid (allele type discriminating information on the region containing the sequence shown in SEQ ID NO: 15).

(16) The nucleic acid contained in the gene sample is the 134 th, 135 th, 137 th, 138 th, 142 th, 165 th, 183 th, 219 th, 232 th, 293 among the base sequence represented by SEQ ID NO: 16 Information about which nucleotide sequence of any of the allelic types shown in Table 17 is the nucleic acid containing the nucleotide sequence of any of the first and 332th nucleotides or the 332th nucleotide (region containing the sequence shown in SEQ ID NO: 16) Allele type discrimination information).

(17) The nucleic acid contained in the gene sample is a base sequence containing any one or a plurality of bases of the 65th, 158th, 236th, 256th, 474th, among the base sequences represented by SEQ ID NO: 17. 18. Information on which allele type of nucleotide sequence shown in 18 is a nucleic acid (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 17).

(18) The nucleic acid contained in the gene sample is any one of the allele types shown in Table 19, wherein the base sequence containing any one or a plurality of bases of the 96th, 133th of the base sequence shown in SEQ ID NO: 18 Information about whether the nucleic acid has a base sequence (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 18).

(19) Table 20 shows the nucleotide sequences of nucleic acids contained in the gene sample, including any of the 261st, 282nd, 371st, 440th or multiple nucleotides of the nucleotide sequence represented by SEQ ID NO: 19. Information on which nucleic acid possesses the base sequence of which allele type (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 19).

(20) The nucleic acid contained in the gene sample is any of the allele types shown in Table 21, wherein the nucleotide sequence containing any one or a plurality of bases of the 247th and 362th positions in the base sequence represented by SEQ ID NO: 20 Information about whether the nucleic acid has a base sequence (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 20).

(21) Table 22 shows the nucleotide sequences in which the nucleic acid contained in the gene sample includes any or more of the 82nd, 91st, 139th, 140th bases of the base sequence represented by SEQ ID NO: 21 Information about which nucleic acid possesses the base sequence of which allele type (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 21).

(22) The nucleic acid contained in the gene sample has the base sequence of any allele type shown in Table 23, wherein the base sequence containing the 359th to 364th bases of the base sequence shown in SEQ ID NO: 22 Information about whether it is a nucleic acid (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 22).

(23) The nucleic acid contained in the gene sample is any of the allele types shown in Table 24, wherein the base sequence containing any one or more of the 420th and 485th bases of the base sequence shown in SEQ ID NO: 23 Information about whether the nucleic acid has a base sequence (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 23).

(24) The nucleic acid contained in the gene sample is 189th, 209th, 218th, 260th, 283rd, 315th, 379th, 385th, 551th, 551th, 597 out of the base sequence represented by SEQ ID NO: 24 Information about which nucleotide sequence of any of the allele types shown in Table 25 is included in the base sequence containing any one or a plurality of bases (allele type relating to the region containing the sequence shown in SEQ ID NO: 24) Discriminating information).

(25) The nucleic acid contained in the gene sample contains any one or a plurality of bases of the 62nd, 96th, 171st, 176th, 222th, 228th, 273th of the base sequence represented by SEQ ID NO: 25 Information about which allele type of the nucleotide sequence shown in Table 26 is the nucleic acid having the nucleotide sequence to be contained (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 25).

(26) The nucleic acid contained in the gene sample contains any one or a plurality of bases of the 57th, 149th, 221nd, 303th, 350th, 400th, 434th of the base sequence represented by SEQ ID NO: 26 Information about which allele type of the nucleotide sequence shown in Table 27 is the nucleic acid having the nucleotide sequence to be contained (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 26).

(27) The nucleic acid contained in the gene sample has a base sequence containing any one or a plurality of bases of the 80th, 116th to 122nd, 204th, 249th, 389th of the base sequence represented by SEQ ID NO: 27 Information about which of the allele types shown in Table 28 is a nucleic acid (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 27).

(28) The nucleic acid contained in the gene sample is represented by a nucleotide sequence comprising any of the 83rd, 169th, 193rd, 215th, 232nd or a plurality of bases in the nucleotide sequence represented by SEQ ID NO: 28. Information on which nucleic acid possesses the base sequence of which allele type shown in 29 (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 28).

(29) The nucleic acid contained in the gene sample is a nucleic acid having a base sequence of any allele type shown in Table 30, wherein the base sequence containing the 309th base is the base sequence shown in SEQ ID NO: 29 Information about the (allele type discrimination information regarding the region including the sequence shown in SEQ ID NO: 29).

(30) The nucleic acid contained in the gene sample is a nucleic acid having a base sequence of any allele type shown in Table 31, wherein the base sequence containing the 216th base in the base sequence shown in SEQ ID NO: 30 Information about the information (allele type discrimination information regarding the region including the sequence shown in SEQ ID NO: 30).

(31) The nucleic acid contained in the gene sample is a nucleic acid having a base sequence of any allele type shown in Table 32, wherein the base sequence containing the 162nd base is the base sequence shown in SEQ ID NO: 31 Information about allele type (allele type discrimination information related to the region including the sequence shown in SEQ ID NO: 31).

(32) The nucleic acid contained in the gene sample is any of the allele types shown in Table 33, wherein the base sequence containing any or more of the 34th and 336th base sequences of the base sequence shown in SEQ ID NO: 32 Information about whether the nucleic acid has a base sequence (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 32).

(33) The nucleic acid contained in the gene sample is a nucleic acid having a base sequence of any allele type shown in Table 34, wherein the base sequence containing the 361st base among the base sequence shown in SEQ ID NO: 33 Information about the (allele type discrimination information regarding the region including the sequence shown in SEQ ID NO: 33).

  The above methods (1) to (33) can be obtained by making full use of means that are sensitive to the presence of SNPs. Specifically, a direct sequencing method, a DHPLC (Denaturing High Performance Liquid Chromatography) method, a method using a Surveyor Nuclease, a TaqMan PCR method, an Invader method, a primer extension method, an SSCP (Single Strand Condrum method). Examples of the method used can be given. About the method using DHPLC method and Surveyor Nuclease, it can carry out according to the protocol using the product of Transgenomic (USA). Regarding the TaqMan PCR method and the Invader method, for example, “Strategy of SNP gene polymorphism” (edited by Yusuke Nakamura, Nakayama Shoten) p. 94-105 and the primer extension method, for example, “Strategy of SNP gene polymorphism” (edited by Yusuke Nakamura, Nakayama Shoten) p. Each of them can be carried out according to the description of 106-117. The SSCP method can be carried out according to the protocol using, for example, a BioRad (USA) DCode system. Regarding the method using a DNA chip, for example, “SNP gene polymorphism strategy” (edited by Yusuke Nakamura, Nakayama Shoten) p. It can carry out according to description of 128-135. In this case, as an oligonucleotide probe to be attached to the DNA chip, 5 to 50 nucleotides including a base in which a SNP described later is present can be used. As the most typical and preferred means, a region containing any one of SEQ ID NOS: (1) to (33) is amplified using a gene amplification primer described below, and BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and GeneAmp PCR System 9700 (Applied Biosystems), the DNA base sequence of the gene amplification product is determined by a direct sequencing method according to a conventional method, and used as a method for discriminating a desired allele type. It is done.

  A general multivariate analysis method can be used for classification of yeasts using the allele type discrimination method. Specific examples include cluster analysis, principal component analysis, and discriminant analysis. If the details of the cluster analysis method are specifically described, any of the nearest neighbor method, the farthest neighbor method, UPGMA (group average method), WPGMA (weighted average method), WARD method and the like can be used. These multivariate analyzes can be easily performed using commercially available statistical software such as STATISTICA, SPSS, and SAS. As the most typical and preferred means, replace allele information in (1) to (33) with homozygous allele type 1 and -1, heterozygous allele type 0, and replace STATISTICA with A method of performing a cluster analysis by using the UPGMA method can be listed.

  The identification of yeast strains refers to determining the allele type and determining the difference between the allele type and the yeast with the known allele type. It is classified into one of the above types according to the allele type of yeast that has been newly classified into the type of type section and newly determined for the allele type. As classification of yeast, for example, classifications of Type 1 to Type 14 described in Table 1 to Table 34 can be mentioned.

  For the selection of yeast for sulfite-producing ability, hydrogen sulfide-producing ability, ester-forming ability, and higher alcohol-producing ability, the SNP of the region containing the sequence shown in SEQ ID NO: 17 is used as an index, and shown in SEQ ID NOS: 1 and 2 It is possible to discriminate using the SNP of the region including any of the sequences as an index. Specifically, the discrimination of the allele type can be performed based on information represented by any combination of the following (1) and (2) and (3). By determining the following allele types, it is possible to predict the characteristics of yeast strains related to sulfite production, hydrogen sulfide production, ester production, higher alcohol production, and select yeast strains with the desired characteristics. can do. Desirable properties include the property that one or more properties of sulfite generating ability, hydrogen sulfide generating ability, ester generating ability, higher alcohol generating ability are high or low.

(1) The nucleic acid contained in the gene sample is any one of the nucleotide sequences shown in Table 18 including the 65th, 158th, 236th, 256th, and 474th bases of the nucleotide sequence shown in SEQ ID NO: 17. Information on whether the nucleic acid has a base sequence of the allele type (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 17).

(2) The nucleic acid contained in the gene sample is any one of the nucleotide sequences shown in Table 2 including the 53rd, 170th, 185th, 239th, and 298th bases of the nucleotide sequence represented by SEQ ID NO: 1. Information on whether the nucleic acid possesses the base sequence of the allele type (allele type discrimination information relating to the region containing the sequence shown in SEQ ID NO: 1).

(3) The nucleic acid contained in the gene sample has the base sequence of any allele type shown in Table 3 in which the base sequence containing the 104th and 321st bases among the base sequence shown in SEQ ID NO: 2 Information about whether it is a nucleic acid (allele type discrimination information regarding the region containing the sequence shown in SEQ ID NO: 2).

  As described above, the method for identifying the strain has been described taking the example of the top fermenting yeast belonging to Saccharomyces cerevisiae and the bottom fermenting yeast belonging to Saccharomyces pastorianus. In the present invention, the yeast belongs to Saccharomyces cerevisiae or Saccharomyces pastorianus. Any yeast can be used as long as it is yeast. For example, the present invention can be applied to sake yeast, wine yeast, shochu yeast, and whiskey yeast as well as practical yeasts such as top fermentation yeast and bottom fermentation yeast.

  The present invention includes primers used to discriminate the above alleles, identify yeast strains, classify yeast strains, or select yeasts.

  A primer is used to amplify DNA containing a specific single nucleotide polymorphism, and is a pair of primers consisting of a primer consisting of the 5 'base sequence of the single nucleotide polymorphism site and a primer consisting of the 3' base sequence Can be designed as a set. The number of bases constituting the primer is 5 to 50, preferably 10 to 30, and more preferably 15 to 25. The length of the base sequence to be amplified is not limited. The single nucleotide polymorphism sites of the present invention are present in 33 regions of yeast genomic DNA, and the total is 148 sites. The primer only needs to be able to amplify a base sequence including at least one site of 148 single nucleotide polymorphism sites. In the case of a single nucleotide polymorphic site present in the vicinity, a base sequence including a plurality of single nucleotide polymorphic sites may be amplified with a pair of primers. Amplification of DNA using a primer can be performed by a known method.

Examples of the primer set of the present invention include the following primer sets.
It is at least a pair of primer sets used for amplification of a DNA fragment containing a single nucleotide polymorphic site on yeast genomic DNA, wherein the single nucleotide polymorphic site is 148 of (1) (a) to (33) (a) below. At least one of the single nucleotide polymorphic sites, wherein the primer is a single nucleotide polymorphic site of the following (1) (a) to (33) (a) of the nucleotide sequences of SEQ ID NOs: 1 to 33 A pair of primer sets that can be used to amplify a DNA fragment, consisting of a partial sequence consisting of 10 to 30 bases present on the 3 ′ side and 5 ′ side of at least one polymorphic site among them:

(1) From SEQ ID NO: 1 (A) to (G):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 53;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 140;
(C) a single nucleotide polymorphic site where base C or T is at the position of base number 170,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 185,
(E) a single nucleotide polymorphic site that is base A or G present at the position of base number 225;
(F) a single nucleotide polymorphic site that is base G or T present at the position of base number 239, and (g) a single nucleotide polymorphic site that is base A or G present at the position of base number 298;

(2) SEQ ID NO: 2, (A) to (E):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 104;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 304,
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 321; and (e) a single nucleotide polymorphism site that is base A or G at the position of base number 322;

(3) (A) to (C) of SEQ ID NO: 3:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 125;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 145;
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 3 bases exists at positions of base numbers 351 to 354;

(4) SEQ ID NO: 4, (A) to (C):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 67;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 74;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 160;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 268,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 324;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 446;
(G) a single nucleotide polymorphism site that is base G or T present at the position of base number 453, and (co) a single nucleotide polymorphism site that is base A or G present at the position of base number 466;

(5) (A) and (I) of SEQ ID NO: 5:
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 431, and (a) a single nucleotide polymorphic site that is base C or T present at the position of base number 464;

(6) SEQ ID NO: 6, (A) to (S):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 75;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 185,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 216,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 217;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 253,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 318,
(H) a single nucleotide polymorphic site that is base A or T present at the position of base number 441;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 444;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 445;
(Sa) a single nucleotide polymorphic site that is base C or T present at the position of base number 463, and (f) a single nucleotide polymorphic site that is base C or T present at the position of base number 493;

(7) From SEQ ID NO: 7, (a) to (f):
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 213,
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 243;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 290,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 348;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 350, and (f) a single nucleotide polymorphic site which is base C or T present at the position of base number 378;

(8) (A) to (E) of SEQ ID NO: 8:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 88;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 152;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 161;
(D) a single nucleotide polymorphism site that is base A or G present at the position of base number 309, and (e) a single nucleotide polymorphism site that is base C or T at the position of base number 381;

(9) From SEQ ID NO: 9, (a) to (c):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 66;
(A) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 229, and (c) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 262;

(10) (A) to (C) of SEQ ID NO: 10:
(A) a single nucleotide polymorphic site which is a deletion or non-deletion of 1 base T existing at positions of base numbers 163 to 170;
(A) a single nucleotide polymorphic site that is a deletion or non-deletion of one base A present at positions of base numbers 309 to 317, and (c) one of bases A or C present at the position of base number 356 Nucleotide polymorphism site;

(11) From SEQ ID NO: 11, (A) to (G):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 69;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 93;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 111;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 208;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 210;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 408, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 429;

(12) From SEQ ID NO: 12, (a) to (c):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(A) a single nucleotide polymorphic site that is base C or G present at the position of base number 151, and (c) a single nucleotide polymorphic site that is base C or T present at the position of base number 343;

(13) (A) to (C) of SEQ ID NO: 13:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 126;
(A) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 201, and (c) a single nucleotide polymorphic site which is the base A or G present at the position of the base number 264;

(14) (A) to (C) of SEQ ID NO: 14:
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 132;
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 185, and (c) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;

(15) From SEQ ID NO: 15, (a) to (e):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 101;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 1 base A exists at positions of base numbers 188 to 192,
(D) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 280, and (e) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 338;

(16) (A) to (SA) of SEQ ID NO: 16:
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 134;
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 135;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 137,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 138;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 142;
(F) a single nucleotide polymorphic site which is base A or G present at the position of base number 165,
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(H) a single nucleotide polymorphic site which is base A or C present at the position of base number 219;
(G) a single nucleotide polymorphic site which is base C or G present at the position of base number 232;
(Co) a single nucleotide polymorphic site which is base A or G present at the position of base number 293, and (sa) a single nucleotide polymorphic site which is base C or T present at the position of base number 332;

(17) From SEQ ID NO: 17, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 65;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphic site which is base G or T present at the position of base number 236,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 256, and (e) a single nucleotide polymorphic site which is base C or T present at the position of base number 474;

(18) (A) and (I) of SEQ ID NO: 18:
(A) a single nucleotide polymorphism site which is the base C or T present at the position of the base number 96, and (a) a single nucleotide polymorphism site which is the base C or T which is present at the position of the base number 133;

(19) (A) to (D) of SEQ ID NO: 19:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 261,
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 282;
(C) a single nucleotide polymorphic site that is base C or T present at the position of base number 371, and (d) a single nucleotide polymorphic site that is base C or T present at the position of base number 440;

(20) (A) and (A) of SEQ ID NO: 20:
(A) a single nucleotide polymorphism site that is base A or G present at the position of base number 247, and (b) a single nucleotide polymorphism site that is base C or T present at the position of base number 362;

(21) From SEQ ID NO: 21, (a) to (d):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 82;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 91;
(C) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 139, and (d) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 140;
(22) (a) a single nucleotide polymorphism site which is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;

(23) (A) and (I) of SEQ ID NO: 23:
(A) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 420, and (a) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 485;

(24) (A) to (C) of SEQ ID NO: 24:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 209,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 218,
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 260;
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 283,
(F) a single nucleotide polymorphic site which is base C or G present at the position of base number 315,
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 379;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 385,
(G) a single nucleotide polymorphic site which is base A or C present at the position of base number 551, and (co) a single nucleotide polymorphic site which is base C or T present at the position of base number 597;

(25) (A) to (G) of SEQ ID NO: 25:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 62;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 96;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 171;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 176,
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 222;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 228, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 273;

(26) From SEQ ID NO: 26, (a) to (g):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 57;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base A or T present at the position of base number 221;
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 303;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 350;
(F) a single nucleotide polymorphic site which is base A or T present at the position of base number 400, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 434;

(27) (A) to (E) of SEQ ID NO: 27:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 80;
(A) a single nucleotide polymorphism site which is a deletion or non-deletion of base A existing at positions from base numbers 116 to 122;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 204;
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 249, and (e) a single nucleotide polymorphism site that is base G or T at the position of base number 389;

(28) From SEQ ID NO: 28, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 83;
(A) a single nucleotide polymorphic site which is base C or G present at the position of base number 169,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 193,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 215, and (e) a single nucleotide polymorphic site which is base A or G present at the position of base number 232;

(29) (a) a single nucleotide polymorphic site which is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 216 of SEQ ID NO: 30;

(31) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 162 of SEQ ID NO: 31;
(32) (A) and (I) of SEQ ID NO: 32:
(A) a single nucleotide polymorphic site that is base C or T present at the position of base number 34, and (a) a single nucleotide polymorphic site that is base A or G present at the position of base number 336;
(33) (a) A single nucleotide polymorphic site which is base A or G present at the position of base number 361 in SEQ ID NO: 33.

  Furthermore, a primer set capable of amplifying a sequence containing all of the single nucleotide polymorphism sites contained in the nucleotide sequences shown in SEQ ID NOs: 1 to 33 at a time is also included. Examples of such primer sets include the following primer sets: It is done.

A primer set comprising a pair of primer sets consisting of at least one of the following combinations (1) to (33):
(1) a primer on the 5 ′ side of base number 53 of SEQ ID NO: 1 and a primer on the 3 ′ side of base number 298 of SEQ ID NO: 1;
(2) a primer on the 5 ′ side of base number 104 of SEQ ID NO: 2 and a primer on the 3 ′ side of base number 322 of SEQ ID NO: 2;
(3) a primer on the 5 ′ side of base number 125 of SEQ ID NO: 3 and a primer on the 3 ′ side of base number 354 of SEQ ID NO: 3;
(4) a primer on the 5 ′ side of base number 67 of SEQ ID NO: 4 and a primer on the 3 ′ side of base number 466 of SEQ ID NO: 4;
(5) a primer on the 5 ′ side of base number 431 of SEQ ID NO: 5 and a primer on the 3 ′ side of base number 464 of SEQ ID NO: 5;
(6) a primer on the 5 ′ side of base number 75 of SEQ ID NO: 6 and a primer on the 3 ′ side of base number 493 of SEQ ID NO: 6;
(7) a primer on the 5 ′ side of base number 213 of SEQ ID NO: 7 and a primer on the 3 ′ side of base number 378 of SEQ ID NO: 7;
(8) a primer on the 5 ′ side of base number 88 of SEQ ID NO: 8 and a primer on the 3 ′ side of base number 381 of SEQ ID NO: 8;
(9) a primer on the 5 ′ side of base number 66 of SEQ ID NO: 9 and a primer on the 3 ′ side of base number 262 of SEQ ID NO: 9;
(10) a primer on the 5 ′ side of base number 163 of SEQ ID NO: 10 and a primer on the 3 ′ side of base number 356 of SEQ ID NO: 10;
(11) a primer on the 5 ′ side of base number 69 of SEQ ID NO: 11 and a primer on the 3 ′ side of base number 429 of SEQ ID NO: 11;
(12) a primer on the 5 ′ side of base number 104 of SEQ ID NO: 12 and a primer on the 3 ′ side of base number 343 of SEQ ID NO: 12;
(13) a primer on the 5 ′ side of base number 126 of SEQ ID NO: 13 and a primer on the 3 ′ side of base number 264 of SEQ ID NO: 13;
(14) a primer on the 5 ′ side of base number 132 of SEQ ID NO: 14 and a primer on the 3 ′ side of base number 189 of SEQ ID NO: 14;
(15) a primer on the 5 ′ side of base number 101 of SEQ ID NO: 15 and a primer on the 3 ′ side of base number 338 of SEQ ID NO: 15;
(16) a primer on the 5 ′ side of base number 134 of SEQ ID NO: 16 and a primer on the 3 ′ side of base number 332 of SEQ ID NO: 16;
(17) a primer on the 5 ′ side of base number 65 of SEQ ID NO: 17 and a primer on the 3 ′ side of base number 474 of SEQ ID NO: 17;
(18) a primer on the 5 ′ side of base number 96 of SEQ ID NO: 18 and a primer on the 3 ′ side of base number 133 of SEQ ID NO: 18;
(19) a primer on the 5 ′ side of base number 261 of SEQ ID NO: 19 and a primer on the 3 ′ side of base number 440 of SEQ ID NO: 19;
(20) a primer on the 5 ′ side of base number 247 of SEQ ID NO: 20 and a primer on the 3 ′ side of base number 362 of SEQ ID NO: 20;
(21) a primer on the 5 ′ side of base number 82 in SEQ ID NO: 21 and a primer on the 3 ′ side of base number 140 in SEQ ID NO: 21;
(22) a primer on the 5 ′ side of base number 359 of SEQ ID NO: 22 and a primer on the 3 ′ side of base number 364 of SEQ ID NO: 22;
(23) a primer on the 5 ′ side of base number 420 of SEQ ID NO: 23 and a primer on the 3 ′ side of base number 485 of SEQ ID NO: 23;
(24) a primer on the 5 ′ side of base number 189 of SEQ ID NO: 24 and a primer on the 3 ′ side of base number 597 of SEQ ID NO: 24;
(25) a primer on the 5 ′ side of base number 62 of SEQ ID NO: 25 and a primer on the 3 ′ side of base number 273 of SEQ ID NO: 25;
(26) a primer on the 5 ′ side of base number 57 of SEQ ID NO: 26 and a primer on the 3 ′ side of base number 434 of SEQ ID NO: 26;
(27) a primer on the 5 ′ side of base number 80 of SEQ ID NO: 27 and a primer on the 3 ′ side of base number 389 of SEQ ID NO: 27;
(28) a primer on the 5 ′ side of base number 83 of SEQ ID NO: 28 and a primer on the 3 ′ side of base number 232 of SEQ ID NO: 28;
(29) a primer on the 5 ′ side of base number 309 of SEQ ID NO: 29 and a primer on the 3 ′ side of base number 309 of SEQ ID NO: 29;
(30) a primer on the 5 ′ side of base number 216 of SEQ ID NO: 30 and a primer on the 3 ′ side of base number 216 of SEQ ID NO: 30;
(31) a primer on the 5 ′ side of base number 162 of SEQ ID NO: 31 and a primer on the 3 ′ side of base number 162 of SEQ ID NO: 31;
(32) a primer on the 5 ′ side of base number 34 of SEQ ID NO: 32 and a primer on the 3 ′ side of base number 336 of SEQ ID NO: 32;
(33) A primer on the 5 ′ side of base number 361 of SEQ ID NO: 33 and a primer on the 3 ′ side of base number 361 of SEQ ID NO: 33

  An example of the primer set is a sequence that can amplify all the sequences of SEQ ID NOs: 1 to 33, and is a combination of the sequences shown below.

A primer set consisting of at least one combination of the following combinations (1) to (33):
(1) A combination of SEQ ID NO: 34 and SEQ ID NO: 35;
(2) a combination of SEQ ID NO: 36 and SEQ ID NO: 37;
(3) a combination of SEQ ID NO: 38 and SEQ ID NO: 39;
(4) a combination of SEQ ID NO: 40 and SEQ ID NO: 41;
(5) a combination of SEQ ID NO: 42 and SEQ ID NO: 43;
(6) a combination of SEQ ID NO: 44 and SEQ ID NO: 45;
(7) a combination of SEQ ID NO: 46 and SEQ ID NO: 47;
(8) A combination of SEQ ID NO: 48 and SEQ ID NO: 49;
(9) Combination of SEQ ID NO: 50 and SEQ ID NO: 51;
(10) A combination of SEQ ID NO: 52 and SEQ ID NO: 53;
(11) A combination of SEQ ID NO: 54 and SEQ ID NO: 55;
(12) A combination of SEQ ID NO: 56 and SEQ ID NO: 57;
(13) A combination of SEQ ID NO: 58 and SEQ ID NO: 59;
(14) a combination of SEQ ID NO: 60 and SEQ ID NO: 61;
(15) a combination of SEQ ID NO: 62 and SEQ ID NO: 63;
(16) A combination of SEQ ID NO: 64 and SEQ ID NO: 65;
(17) A combination of SEQ ID NO: 66 and SEQ ID NO: 67;
(18) A combination of SEQ ID NO: 68 and SEQ ID NO: 69;
(19) A combination of SEQ ID NO: 70 and SEQ ID NO: 71;
(20) a combination of SEQ ID NO: 72 and SEQ ID NO: 73;
(21) A combination of SEQ ID NO: 74 and SEQ ID NO: 75;
(22) a combination of SEQ ID NO: 76 and SEQ ID NO: 77;
(23) A combination of SEQ ID NO: 78 and SEQ ID NO: 79;
(24) A combination of SEQ ID NO: 80 and SEQ ID NO: 81;
(25) a combination of SEQ ID NO: 82 and SEQ ID NO: 83;
(26) a combination of SEQ ID NO: 84 and SEQ ID NO: 85;
(27) A combination of SEQ ID NO: 86 and SEQ ID NO: 87;
(28) A combination of SEQ ID NO: 88 and SEQ ID NO: 89;
(29) a combination of SEQ ID NO: 90 and SEQ ID NO: 91;
(30) a combination of SEQ ID NO: 92 and SEQ ID NO: 93;
(31) A combination of SEQ ID NO: 94 and SEQ ID NO: 95;
(32) a combination of SEQ ID NO: 96 and SEQ ID NO: 97;
(33) A combination of SEQ ID NO: 98 and SEQ ID NO: 99.

  Furthermore, the present invention includes an oligonucleotide or a label thereof used for detecting and discriminating the above alleles, identifying yeast strains, classifying yeast strains, or selecting yeasts. The oligonucleotide consists of a DNA fragment comprising a base sequence containing a single nucleotide polymorphic site of yeast or a base sequence complementary to the base sequence. The oligonucleotide can be used as a probe for detecting a single nucleotide polymorphism. The number of bases constituting the oligonucleotide used for detection and discrimination of alleles is 5 to 50, preferably 10 to 30, and more preferably 15 to 25. Allele discrimination can be performed by a hybridization assay using the probe. The oligonucleotide of the present invention can be prepared by chemical synthesis or can be prepared as an amplification product obtained by amplifying a gene by PCR using the above primers. The probe of the present invention may be labeled with a fluorescent substance, an enzyme, a radioisotope, a chemiluminescent substance or the like for detection. As a labeling substance used for labeling, a known substance can be used and labeled by a known method. Examples of the fluorescent substance include Cy3, Cy5, rhodamine, fluorescein and the like.

Examples of the oligonucleotide of the present invention include the following oligonucleotides.
An oligonucleotide consisting of a DNA fragment containing a single nucleotide polymorphic site on yeast genomic DNA or a label thereof, and 148 single nucleotide polymorphic sites from the following (1) (a) to (33) (a) At least one of the following single nucleotide polymorphism sites (1) (a) to (33) (a) of the nucleotide sequences of SEQ ID NOs: 1 to 33: An oligonucleotide consisting of a partial sequence consisting of 10 to 30 bases containing one polymorphic site or a sequence complementary to the partial sequence, or a label thereof:

(1) From SEQ ID NO: 1 (A) to (G):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 53;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 140;
(C) a single nucleotide polymorphic site where base C or T is at the position of base number 170,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 185,
(E) a single nucleotide polymorphic site that is base A or G present at the position of base number 225;
(F) a single nucleotide polymorphic site that is base G or T present at the position of base number 239, and (g) a single nucleotide polymorphic site that is base A or G present at the position of base number 298;

(2) SEQ ID NO: 2, (A) to (E):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 104;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 304,
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 321; and (e) a single nucleotide polymorphism site that is base A or G at the position of base number 322;

(3) (A) to (C) of SEQ ID NO: 3:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 125;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 145;
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 3 bases exists at positions of base numbers 351 to 354;

(4) SEQ ID NO: 4, (A) to (C):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 67;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 74;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 160;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 268,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 324;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 446;
(G) a single nucleotide polymorphism site that is base G or T present at the position of base number 453, and (co) a single nucleotide polymorphism site that is base A or G present at the position of base number 466;

(5) (A) and (I) of SEQ ID NO: 5:
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 431, and (a) a single nucleotide polymorphic site that is base C or T present at the position of base number 464;

(6) SEQ ID NO: 6, (A) to (S):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 75;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 185,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 216,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 217;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 253,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 318,
(H) a single nucleotide polymorphic site that is base A or T present at the position of base number 441;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 444;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 445;
(Sa) a single nucleotide polymorphic site that is base C or T present at the position of base number 463, and (f) a single nucleotide polymorphic site that is base C or T present at the position of base number 493;

(7) From SEQ ID NO: 7, (a) to (f):
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 213,
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 243;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 290,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 348;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 350, and (f) a single nucleotide polymorphic site which is base C or T present at the position of base number 378;

(8) (A) to (E) of SEQ ID NO: 8:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 88;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 152;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 161;
(D) a single nucleotide polymorphism site that is base A or G present at the position of base number 309, and (e) a single nucleotide polymorphism site that is base C or T at the position of base number 381;

(9) From SEQ ID NO: 9, (a) to (c):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 66;
(A) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 229, and (c) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 262;

(10) (A) to (C) of SEQ ID NO: 10:
(A) a single nucleotide polymorphic site which is a deletion or non-deletion of 1 base T existing at positions of base numbers 163 to 170;
(A) a single nucleotide polymorphic site that is a deletion or non-deletion of one base A present at positions of base numbers 309 to 317, and (c) one of bases A or C present at the position of base number 356 Nucleotide polymorphism site;

(11) From SEQ ID NO: 11, (A) to (G):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 69;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 93;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 111;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 208;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 210;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 408, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 429;

(12) From SEQ ID NO: 12, (a) to (c):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(A) a single nucleotide polymorphic site that is base C or G present at the position of base number 151, and (c) a single nucleotide polymorphic site that is base C or T present at the position of base number 343;

(13) (A) to (C) of SEQ ID NO: 13:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 126;
(A) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 201, and (c) a single nucleotide polymorphic site which is the base A or G present at the position of the base number 264;

(14) (A) to (C) of SEQ ID NO: 14:
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 132;
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 185, and (c) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;

(15) From SEQ ID NO: 15, (a) to (e):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 101;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 1 base A exists at positions of base numbers 188 to 192,
(D) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 280, and (e) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 338;

(16) (A) to (SA) of SEQ ID NO: 16:
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 134;
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 135;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 137,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 138;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 142;
(F) a single nucleotide polymorphic site which is base A or G present at the position of base number 165,
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(H) a single nucleotide polymorphic site which is base A or C present at the position of base number 219;
(G) a single nucleotide polymorphic site which is base C or G present at the position of base number 232;
(Co) a single nucleotide polymorphic site which is base A or G present at the position of base number 293, and (sa) a single nucleotide polymorphic site which is base C or T present at the position of base number 332;

(17) From SEQ ID NO: 17, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 65;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphic site which is base G or T present at the position of base number 236,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 256, and (e) a single nucleotide polymorphic site which is base C or T present at the position of base number 474;

(18) (A) and (I) of SEQ ID NO: 18:
(A) a single nucleotide polymorphism site which is the base C or T present at the position of the base number 96, and (a) a single nucleotide polymorphism site which is the base C or T which is present at the position of the base number 133;

(19) (A) to (D) of SEQ ID NO: 19:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 261,
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 282;
(C) a single nucleotide polymorphic site that is base C or T present at the position of base number 371, and (d) a single nucleotide polymorphic site that is base C or T present at the position of base number 440;

(20) (A) and (A) of SEQ ID NO: 20:
(A) a single nucleotide polymorphism site that is base A or G present at the position of base number 247, and (b) a single nucleotide polymorphism site that is base C or T present at the position of base number 362;

(21) From SEQ ID NO: 21, (a) to (d):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 82;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 91;
(C) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 139, and (d) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 140;

(22) (a) a single nucleotide polymorphism site which is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) (A) and (I) of SEQ ID NO: 23:
(A) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 420, and (a) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 485;

(24) (A) to (C) of SEQ ID NO: 24:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 209,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 218,
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 260;
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 283,
(F) a single nucleotide polymorphic site which is base C or G present at the position of base number 315,
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 379;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 385,
(G) a single nucleotide polymorphic site which is base A or C present at the position of base number 551, and (co) a single nucleotide polymorphic site which is base C or T present at the position of base number 597;

(25) (A) to (G) of SEQ ID NO: 25:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 62;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 96;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 171;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 176,
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 222;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 228, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 273;

(26) From SEQ ID NO: 26, (a) to (g):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 57;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base A or T present at the position of base number 221;
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 303;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 350;
(F) a single nucleotide polymorphic site which is base A or T present at the position of base number 400, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 434;

(27) (A) to (E) of SEQ ID NO: 27:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 80;
(A) a single nucleotide polymorphism site which is a deletion or non-deletion of base A existing at positions from base numbers 116 to 122;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 204;
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 249, and (e) a single nucleotide polymorphism site that is base G or T at the position of base number 389;

(28) From SEQ ID NO: 28, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 83;
(A) a single nucleotide polymorphic site which is base C or G present at the position of base number 169,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 193,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 215, and (e) a single nucleotide polymorphic site which is base A or G present at the position of base number 232;

(29) (a) a single nucleotide polymorphic site which is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 162 of SEQ ID NO: 31;
(32) (A) and (I) of SEQ ID NO: 32:
(A) a single nucleotide polymorphic site that is base C or T present at the position of base number 34, and (a) a single nucleotide polymorphic site that is base A or G present at the position of base number 336;
(33) (a) A single nucleotide polymorphic site which is base A or G present at the position of base number 361 in SEQ ID NO: 33.

  The oligonucleotide or the labeled product thereof may contain a plurality of the single nucleotide polymorphism sites. In this case, the oligonucleotide or label thereof is a partial sequence of the nucleotide sequence of SEQ ID NOs: 1 to 33, and a sequence containing a plurality of single nucleotide polymorphism sites contained in the sequence.

  Furthermore, the present invention includes an immobilized substrate on which the oligonucleotide is immobilized. Various substrates such as a slide glass, a nitrocellulose membrane, and microbeads can be used as the substrate on which the oligonucleotide is immobilized. When a plurality of oligonucleotides are aligned and immobilized on an immobilized substrate, the immobilized substrate can be used as a DNA microarray or a DNA chip. Further, the oligonucleotide may be synthesized on a substrate, or the synthesized oligonucleotide may be immobilized on the substrate. Immobilization on a substrate can be performed using, for example, a commercially available spotter or arrayer, and oligonucleotides can be immobilized by adsorption or binding via covalent bond, and by covalent bond binding. For immobilization, functional groups such as amino groups and SH groups for covalent bonding may be introduced into the substrate surface and the oligonucleotide.

  Allele detection using an immobilized substrate on which an oligonucleotide is immobilized can be performed by a known method.

  The present invention will be specifically described by the following examples, but the present invention is not limited to these examples.

Primer design Primers were designed as described below.
First, genomic DNA was extracted from the bottom fermented yeast KBY011 strain according to the method described by Oshima et al. (Protein Nucleic Acid Enzyme, 1990, Vol. 35, No. 14, p2523-p2541), and the restriction enzyme Sau3AI (Roche Diagnostics) Sucrose density gradient centrifugation according to the method described in 2.80-2.83 of Molecular Cloning 3rd Edition (Sambrook and Russell, Cold Spring Harbor Laboratory Press), about 1000 A DNA fragment having a base pair size was recovered. The recovered DNA was completely digested with the restriction enzyme BamHI and ligated to the BamHI site of pUC118 (Takara Bio) that had been dephosphorylated using DNA Ligation Kit Ver2.1 (Takara Bio). The resulting reaction product was used to transform Escherichia coli Competent High DH5α (Toyobo) according to the attached protocol. The obtained transformant was cultured according to a conventional method, and then the plasmid was collected using a multiscreen-FB plate (Millipore). Using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems), GeneAmp PCR system 9700 DNA sequencing reaction was performed using (Applied Biosystems). M13 Primer M1 and M13 Primer RV (both Takara Bio) were used as primers. The sequencing reaction followed the kit protocol. Unreacted fluorescent dye was removed using a multiscreen-HV plate (Millipore) packed with Sephadex G-50 Superfine (Amersham), and the resulting reactive organism was used using ABI PRISM 3700 DNA Analyzer (Applied Biosystems). DNA base sequencing was performed. Thus, the DNA base sequence of about 6000 transformants was obtained. The result of the obtained DNA base sequence was compared with a known Saccharomyces cerevisiae sequence to confirm the position of the locus on the chromosome.

  From the obtained base sequence, at appropriate intervals on the chromosome map, 114 sites and 123 from the two subgenomes constituting the genome of the bottom-fermenting yeast that is an allopolyploid, namely, Sc type genome and Lg type genome, respectively. A total of 237 chromosomal positions (hereinafter referred to as gene loci) from the entire genome and the entire genome are set, and primer design is performed so that a non-coding region of about 500 base pairs is amplified for each gene locus. It was.

  Since the Sc-type genome is almost identical to the Saccharomyces cerevisiae sequence, 65 loci are set at appropriate intervals on the chromosome map from the known Saccharomyces cerevisiae sequence. Primers were designed for the locus to amplify mainly the non-coding region of the approximately 500 base pair Sc-type genome. In this way, primer design was performed mainly for amplifying non-coding regions at a total of 302 loci, 179 from the Sc-type genome and 123 from the Lg-type genome. Oligonucleotides used as primers were obtained by requesting synthesis from Sigma-Aldrich Japan.

Search for the locus where the SNP exists The search for the locus where the SNP exists was performed as described below.
From the bottom fermentation yeast of 8 strains of # 2, # 5, # 8, # 11, # 15, # 19, # 27, and # 28, the method described by Oshima et al. (Protein Nucleic Acid Enzyme, 1990, Vol. 35, No. 14. p2523 to p2541), genomic DNA was extracted. The obtained genomic DNA was adjusted to a concentration of 10 ng / μl. Gene amplification was performed by GeneAmp PCR system 9700 (Applied Biosystems) using the obtained genomic DNA as a template and the above-described 302 primer pairs and Ex-Taq (Takara Bio). The reaction was performed on a scale of 2 μM for each primer, 3 ng / μl for template DNA, and 10 μl per tube. PCR conditions were 94 ° C. for 5 minutes, followed by 45 cycles of 94 ° C. for 30 seconds, 58 ° C. for 30 seconds, and 72 ° C. for 30 seconds, and finally kept at 72 ° C. for 7 minutes. The sample after the reaction was diluted by adding 30 μl of sterilized water, a part thereof was taken out, and the reaction was confirmed by electrophoresis using a 1% agarose gel.

  DNA sequencing reaction was performed using GeneDamp PCR system 9700 (Applied Biosystems) using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) with the above PCR product as a template. As a primer, one of the primers used in PCR for obtaining a template was used at a concentration of 2.44 μM. The sequencing reaction followed the kit protocol. After the reaction, the unreacted fluorescent dye was removed using a multiscreen-HV plate (Millipore) packed with Sephadex G-50 Superfine (Amersham), and ABI PRISM 3700 DNA Analyzer (Applied Biosystems) was used. DNA base sequencing was performed. For each locus, nucleotide sequence data obtained from DNA of 8 strains of yeast was obtained from ATGC Ver. 4 (Genetics) was assembled on a computer to confirm the presence of SNPs. At that time, attention was also paid to the waveform data of the obtained DNA base sequence, and homozygous and heterozygous were distinguished (FIG. 1).

Thus, by comparing the DNA base sequences of 302 loci of 8 strains of bottom fermenting yeast, 33 loci where SNPs were found in any yeast were selected. The primer combinations necessary for amplifying the region of 33 loci by PCR and the typical sequences obtained by the primer combinations are shown in the following 1 to 33:
1. Primer combination: SEQ ID NO: 34 and 35, amplified sequence: SEQ ID NO: 1;
2. Primer combination: SEQ ID NO: 36 and 37, amplified sequence: SEQ ID NO: 2;
3. Primer combination: SEQ ID NO: 38 and 39, amplified sequence: SEQ ID NO: 3;
4). Primer combination: SEQ ID NO: 40 and 41, amplified sequence: SEQ ID NO: 4;
5. Primer combination: SEQ ID NOs: 42 and 43, amplified sequence: SEQ ID NO: 5;
6). Primer combination: SEQ ID NO: 44 and 45, amplified sequence: SEQ ID NO: 6;
7). Primer combination: SEQ ID NO: 47 and 48, amplified sequence: SEQ ID NO: 7;
8). Primer combination: SEQ ID NO: 48 and 49, amplified sequence: SEQ ID NO: 8;
9. Primer combination: SEQ ID NO: 50 and 51, amplified sequence: SEQ ID NO: 9;
10. Primer combination: SEQ ID NO: 52 and 53, amplified sequence: SEQ ID NO: 10;
11. Primer combination: SEQ ID NO: 54 and 55, amplified sequence: SEQ ID NO: 11;
12 Primer combination: SEQ ID NO: 56 and 57, amplified sequence: SEQ ID NO: 12;
13. Primer combination: SEQ ID NO: 58 and 59, amplified sequence: SEQ ID NO: 13;
14 Primer combination: SEQ ID NO: 60 and 61, amplified sequence: SEQ ID NO: 14;
15. Primer combination: SEQ ID NO: 62 and 63, amplified sequence: SEQ ID NO: 15;
16. Primer combination: SEQ ID NOs: 64 and 65, amplified sequence: SEQ ID NO: 16;
17. Primer combination: SEQ ID NO: 66 and 67, amplified sequence: SEQ ID NO: 17;
18. Primer combination: SEQ ID NO: 68 and 69, amplified sequence: SEQ ID NO: 18;
19. Primer combination: SEQ ID NO: 70 and 71, amplified sequence: SEQ ID NO: 19;
20. Primer combination: SEQ ID NO: 72 and 73, amplified sequence: SEQ ID NO: 20;
21. Primer combination: SEQ ID NO: 74 and 75, amplified sequence: SEQ ID NO: 21;
22. Primer combination: SEQ ID NO: 76 and 77, amplified sequence: SEQ ID NO: 22;
23. Primer combination: SEQ ID NO: 78 and 79, amplified sequence: SEQ ID NO: 23;
24. Primer combination: SEQ ID NO: 80 and 81, Amplified sequence: SEQ ID NO: 24;
25. Primer combination: SEQ ID NO: 82 and 83, amplified sequence: SEQ ID NO: 25;
26. Primer combination: SEQ ID NO: 84 and 85, amplified sequence: SEQ ID NO: 26;
27. Primer combination: SEQ ID NO: 86 and 87, amplified sequence: SEQ ID NO: 27;
28. Primer combination: SEQ ID NO: 88 and 89, amplified sequence: SEQ ID NO: 28;
29. Primer combination: SEQ ID NO: 90 and 91, amplified sequence: SEQ ID NO: 29;
30. Primer combination: SEQ ID NO: 92 and 93, amplified sequence: SEQ ID NO: 30;
31. Primer combination: SEQ ID NO: 94 and 95, amplified sequence: SEQ ID NO: 31;
32. Primer combination: SEQ ID NO: 96 and 97, amplified sequence: SEQ ID NO: 32;
33. Primer combination: SEQ ID NO: 98 and 99, amplified sequence: SEQ ID NO: 33;

Analysis of SNPs of yeast strains and identification of strains by allele type For the loci where 33 selected SNPs were found, 53 bottom fermented yeasts and top side fermented yeasts shown in Table 1 were used in the same manner as described above. The DNA base sequence of yeast was determined. For each gene locus, nucleotide sequence data obtained from the DNA of 53 strains of yeast was obtained from ATGC Ver. 4 (Genetics) was assembled on a computer, and the presence or absence of SNP was investigated while distinguishing homozygous and heterozygous using waveform data. As a result, allele types as shown in Tables 2 to 34 could be obtained.

配列番号１における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 1 and allele pattern of each strain

配列番号２における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 2 and allele pattern of each strain

配列番号３における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 3 and allele pattern of each strain

配列番号４における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 4 and allele pattern of each strain

配列番号５における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 5 and allele pattern of each strain

配列番号６における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 6 and allele pattern of each strain

配列番号７における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 7 and allele pattern of each strain

配列番号８における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 8 and allele pattern of each strain

配列番号９における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 9 and allele pattern of each strain

配列番号１０における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 10 and allele pattern of each strain

配列番号１１における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 11 and allele pattern of each strain

配列番号１２における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 12 and allele pattern of each strain

配列番号１３における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 13 and allele pattern of each strain

配列番号１４における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 14 and allele pattern of each strain

配列番号１５における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 15 and allele pattern of each strain

配列番号１６における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 16 and allele pattern of each strain

配列番号１７における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 17 and allele pattern of each strain

配列番号１８における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 18 and allele pattern of each strain

配列番号１９における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 19 and allele pattern of each strain

配列番号２０における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 20 and allele pattern of each strain

配列番号２１における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 21 and allele pattern of each strain

配列番号２２における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 22 and allele pattern of each strain

配列番号２３における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 23 and allele pattern of each strain

配列番号２４における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 24 and allele pattern of each strain

配列番号２５における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 25 and allele pattern of each strain

配列番号２６における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 26 and allele pattern of each strain

配列番号２７における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 27 and allele pattern of each strain

配列番号２８における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 28 and allele pattern of each strain

配列番号２９における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 29 and allele pattern of each strain

配列番号３０における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 30 and allele pattern of each strain

配列番号３１における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 31 and allele pattern of each strain

配列番号３２における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 32 and allele pattern of each strain

配列番号３３における多型の位置と、各菌株のアレルパターン

Position of polymorphism in SEQ ID NO: 33 and allele pattern of each strain

By combining these allele type differences, it was possible to almost distinguish 53 strains of yeast. It was the following three sets of bottom fermenting yeast that could not be distinguished by the combination of differences in these allele types:
1. # 05 and # 27 shares;
2. # 08 and # 18 shares;
3. # 21 and # 25 shares;
The possibility that these three sets are the same stock cannot be denied.

Classification of yeast strains by utilization of allele types With regard to the 53 bottom fermented yeasts and top fermented yeasts shown in Table 1, 18 regions shown in Table 3, Table 7, Table 14 to Table 25, Table 29, Table 32 to Table 34 Cluster analysis was performed using the data of each polymorphism. The polymorphism data replaced homozygotes with -1 and 1 and heterozygotes with 0. For the cluster analysis, statistical analysis software STATISTICA was used, the UPGMA method was selected, and the analysis was performed according to the instruction manual. FIG. 2 shows the result of analyzing the data of the bottom fermentation yeast and the top fermentation yeast. The bottom fermenting yeast indicated in bold and the top fermenting yeast indicated in fine are classified into different clusters.

FIG. 3 shows the result of cluster analysis performed on the data of only the bottom fermenting yeast strain.
Even when only the data for the bottom fermenting yeast was used, the affinity within the bacterial species of the bottom fermenting yeast could be shown in the dendrogram.

Discovery of allele types related to sulfurous acid, hydrogen sulfide, ester, higher alcohol production ability Among the yeast strains shown in Table 1, # 03, # 04, # 05, # 06, # 07, # 08 # 10, # 12, # 14, # 15, # 16, # 17, # 18, # 19, # 20, # 21, # 22, # 23, A fermentation test was conducted by selecting # 24 strain, # 25 strain, # 26 strain, # 27 strain, # 30 strain, and # 31 strain. The fermentation test was carried out by static culture using a general beer brewing medium as a medium and a glass test tube having a liquid volume of 500 ml and a liquid depth of 100 cm. After culturing for 1 week, various components of the culture solution were measured. The components were measured by titration for sulfurous acid and gas chromatography for hydrogen sulfide, ester and higher alcohol. The relationship between the ability to produce various components during fermentation of the tested yeast and each polymorphism data in the 33 regions shown in Tables 2 to 34 was examined. As a result, among the allele polymorphisms included in SEQ ID NO: 17 shown in Table 18, the strain included in Type 1, that is, the base present at the position of base number 65 in SEQ ID NO: 17 is an A / G heterozygote. The base present at the position of base number 158 is an A / G heterojunction, the base present at the position of base number 236 is a heterojunction of G / T, and the base present at the position of base number 256 is For yeast that is A / G heterozygous and the base at position 474 is C / T heterozygous, allele polymorphisms contained in SEQ ID NO: 1 and SEQ ID NO: 2 and during fermentation It has been found that there is a relationship between the values of the various components produced. Among the test strains, strains whose polymorphic type of the allele included in SEQ ID NO: 17 is Type 1 are as follows: # 04 strain, # 05 strain, # 08 strain, # 14 strain, # 16 strain # 17, # 18, # 21, # 25, # 27, # 30, # 31. Regarding these strains, when the relationship between the allele polymorphisms contained in SEQ ID NO: 1 and SEQ ID NO: 2 and the values of various components generated during fermentation was examined, the allele polymorphisms contained in SEQ ID NO: 1 shown in Table 2 Among them, the strain included in Type 1, that is, the base present at the position of base number 53 of SEQ ID NO: 1 is C, the base present at the position of base number 170 is C, and the base is located at the position of base number 185. The yeast strain in which the base present is A, the base present at the position of base number 239 is G, and the base present at the position of base number 298 is A is a strain included in Type 4, ie, SEQ ID NO: 1 The base present at the position of base number 53 is a C / T heterojunction, the base present at the position of base number 170 is a C / T heterojunction, and the base present at the position of base number 185 is A / G It is a telozygous, the base present at the position of base number 239 is a G / T heterojunction, the base present at the position of base number 298 is an A / G heterozygote and is more sulfite, sulfide than yeast strains It was found that the ability to produce hydrogen, esters and higher alcohols was all high. These results are shown in FIGS. Similarly, among allele polymorphisms contained in SEQ ID NO: 2 shown in Table 3, the strains contained in Type 1 are all more capable of producing sulfite, hydrogen sulfide, esters and higher alcohols than the strains contained in Type 5. It was found. From these results, in order to select a strain having a high ability to produce sulfurous acid, hydrogen sulfide, ester, and higher alcohol, among allele polymorphisms included in SEQ ID NO: 17 shown in Table 18, strains included in Type 1 were selected. Among them, among the allele polymorphisms contained in SEQ ID NO: 1 shown in Table 2, among them, the strain contained in Type 1, and among allele polymorphisms contained in SEQ ID NO: 2 shown in Table 3, Type 1 It was shown that at least any one of the strains contained in the above should be selected. In addition, in order to select a strain having low ability to produce sulfurous acid, hydrogen sulfide, ester and higher alcohol, a strain included in Type 1 is selected from polymorphs of alleles included in SEQ ID NO: 17 shown in Table 18. Among them, among the allele polymorphisms included in SEQ ID NO: 1 shown in Table 2, among strains included in Type 4, and among allele polymorphisms included in SEQ ID NO: 2 shown in Table 3, are included in Type 5 It was shown that at least one of the strains should be selected.

It is a figure which shows the DNA base sequence waveform data of a yeast strain | stump | stock. It is a figure which shows the result of the cluster analysis of a bottom fermentation yeast and a top fermentation yeast. It is a figure which shows the result of the cluster analysis of bottom fermentation yeast. It is a figure which shows the production amount of sulfurous acid and hydrogen sulfide of the yeast strains of Type 1 and Type 2 allele types. It is a figure which shows the production amount of isoamyl acetate and n-propanol of the yeast strains of Type 1 and Type 2 allele types.

SEQ ID NOs: 34 to 99: primers

Claims (31)

It is at least a pair of primer sets used for amplification of a DNA fragment containing a single nucleotide polymorphic site on yeast genomic DNA, wherein the single nucleotide polymorphic site is 148 of (1) (a) to (33) (a) below. At least one of the single nucleotide polymorphic sites, wherein the primer is a single nucleotide polymorphic site of the following (1) (a) to (33) (a) of the nucleotide sequences of SEQ ID NOs: 1 to 33 A pair of primer sets that can be used to amplify a DNA fragment, consisting of a partial sequence consisting of 10 to 30 bases present on the 3 ′ side and 5 ′ side of at least one polymorphic site among them:
(1) From SEQ ID NO: 1 (A) to (G):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 53;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 140;
(C) a single nucleotide polymorphic site where base C or T is at the position of base number 170,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 185,
(E) a single nucleotide polymorphic site that is base A or G present at the position of base number 225;
(F) a single nucleotide polymorphic site that is base G or T present at the position of base number 239, and (g) a single nucleotide polymorphic site that is base A or G present at the position of base number 298;
(2) SEQ ID NO: 2, (A) to (E):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 104;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 304,
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 321; and (e) a single nucleotide polymorphism site that is base A or G at the position of base number 322;
(3) (A) to (C) of SEQ ID NO: 3:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 125;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 145;
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 3 bases exists at positions of base numbers 351 to 354;
(4) SEQ ID NO: 4, (A) to (C):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 67;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 74;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 160;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 268,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 324;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 446;
(G) a single nucleotide polymorphism site that is base G or T present at the position of base number 453, and (co) a single nucleotide polymorphism site that is base A or G present at the position of base number 466;
(5) (A) and (I) of SEQ ID NO: 5:
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 431, and (a) a single nucleotide polymorphic site that is base C or T present at the position of base number 464;
(6) SEQ ID NO: 6, (A) to (S):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 75;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 185,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 216,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 217;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 253,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 318,
(H) a single nucleotide polymorphic site that is base A or T present at the position of base number 441;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 444;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 445;
(Sa) a single nucleotide polymorphic site that is base C or T present at the position of base number 463, and (f) a single nucleotide polymorphic site that is base C or T present at the position of base number 493;
(7) From SEQ ID NO: 7, (a) to (f):
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 213,
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 243;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 290,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 348;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 350, and (f) a single nucleotide polymorphic site which is base C or T present at the position of base number 378;
(8) (A) to (E) of SEQ ID NO: 8:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 88;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 152;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 161;
(D) a single nucleotide polymorphism site that is base A or G present at the position of base number 309, and (e) a single nucleotide polymorphism site that is base C or T at the position of base number 381;
(9) From SEQ ID NO: 9, (a) to (c):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 66;
(A) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 229, and (c) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 262;
(10) (A) to (C) of SEQ ID NO: 10:
(A) a single nucleotide polymorphic site which is a deletion or non-deletion of 1 base T existing at positions of base numbers 163 to 170;
(A) a single nucleotide polymorphic site that is a deletion or non-deletion of one base A present at positions of base numbers 309 to 317, and (c) one of bases A or C present at the position of base number 356 Nucleotide polymorphism site;
(11) From SEQ ID NO: 11, (A) to (G):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 69;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 93;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 111;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 208;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 210;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 408, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 429;
(12) From SEQ ID NO: 12, (a) to (c):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(A) a single nucleotide polymorphic site that is base C or G present at the position of base number 151, and (c) a single nucleotide polymorphic site that is base C or T present at the position of base number 343;
(13) (A) to (C) of SEQ ID NO: 13:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 126;
(A) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 201, and (c) a single nucleotide polymorphic site which is the base A or G present at the position of the base number 264;
(14) (A) to (C) of SEQ ID NO: 14:
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 132;
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 185, and (c) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(15) From SEQ ID NO: 15, (a) to (e):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 101;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 1 base A exists at positions of base numbers 188 to 192,
(D) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 280, and (e) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 338;
(16) (A) to (SA) of SEQ ID NO: 16:
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 134;
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 135;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 137,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 138;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 142;
(F) a single nucleotide polymorphic site which is base A or G present at the position of base number 165,
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(H) a single nucleotide polymorphic site which is base A or C present at the position of base number 219;
(G) a single nucleotide polymorphic site which is base C or G present at the position of base number 232;
(Co) a single nucleotide polymorphic site which is base A or G present at the position of base number 293, and (sa) a single nucleotide polymorphic site which is base C or T present at the position of base number 332;
(17) From SEQ ID NO: 17, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 65;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphic site which is base G or T present at the position of base number 236,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 256, and (e) a single nucleotide polymorphic site which is base C or T present at the position of base number 474;
(18) (A) and (I) of SEQ ID NO: 18:
(A) a single nucleotide polymorphism site which is the base C or T present at the position of the base number 96, and (a) a single nucleotide polymorphism site which is the base C or T which is present at the position of the base number 133;
(19) (A) to (D) of SEQ ID NO: 19:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 261,
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 282;
(C) a single nucleotide polymorphic site that is base C or T present at the position of base number 371, and (d) a single nucleotide polymorphic site that is base C or T present at the position of base number 440;
(20) (A) and (A) of SEQ ID NO: 20:
(A) a single nucleotide polymorphism site that is base A or G present at the position of base number 247, and (b) a single nucleotide polymorphism site that is base C or T present at the position of base number 362;
(21) From SEQ ID NO: 21, (a) to (d):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 82;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 91;
(C) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 139, and (d) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 140;
(22) (a) a single nucleotide polymorphism site which is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) (A) and (I) of SEQ ID NO: 23:
(A) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 420, and (a) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 485;
(24) (A) to (C) of SEQ ID NO: 24:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 209,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 218,
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 260;
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 283,
(F) a single nucleotide polymorphic site which is base C or G present at the position of base number 315,
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 379;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 385,
(G) a single nucleotide polymorphic site which is base A or C present at the position of base number 551, and (co) a single nucleotide polymorphic site which is base C or T present at the position of base number 597;
(25) (A) to (G) of SEQ ID NO: 25:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 62;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 96;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 171;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 176,
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 222;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 228, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 273;
(26) From SEQ ID NO: 26, (a) to (g):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 57;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base A or T present at the position of base number 221;
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 303;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 350;
(F) a single nucleotide polymorphic site which is base A or T present at the position of base number 400, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 434;
(27) (A) to (E) of SEQ ID NO: 27:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 80;
(A) a single nucleotide polymorphism site which is a deletion or non-deletion of base A existing at positions from base numbers 116 to 122;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 204;
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 249, and (e) a single nucleotide polymorphism site that is base G or T at the position of base number 389;
(28) From SEQ ID NO: 28, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 83;
(A) a single nucleotide polymorphic site which is base C or G present at the position of base number 169,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 193,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 215, and (e) a single nucleotide polymorphic site which is base A or G present at the position of base number 232;
(29) (a) a single nucleotide polymorphic site which is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 162 of SEQ ID NO: 31;
(32) (A) and (I) of SEQ ID NO: 32:
(A) a single nucleotide polymorphic site that is base C or T present at the position of base number 34, and (a) a single nucleotide polymorphic site that is base A or G present at the position of base number 336;
(33) (a) A single nucleotide polymorphic site which is base A or G present at the position of base number 361 in SEQ ID NO: 33. The primer set according to claim 1, wherein the pair of primer sets comprises at least one combination of the following combinations (1) to (33):
(1) a primer on the 5 ′ side of base number 53 of SEQ ID NO: 1 and a primer on the 3 ′ side of base number 298 of SEQ ID NO: 1;
(2) a primer on the 5 ′ side of base number 104 of SEQ ID NO: 2 and a primer on the 3 ′ side of base number 322 of SEQ ID NO: 2;
(3) a primer on the 5 ′ side of base number 125 of SEQ ID NO: 3 and a primer on the 3 ′ side of base number 354 of SEQ ID NO: 3;
(4) a primer on the 5 ′ side of base number 67 of SEQ ID NO: 4 and a primer on the 3 ′ side of base number 466 of SEQ ID NO: 4;
(5) a primer on the 5 ′ side of base number 431 of SEQ ID NO: 5 and a primer on the 3 ′ side of base number 464 of SEQ ID NO: 5;
(6) a primer on the 5 ′ side of base number 75 of SEQ ID NO: 6 and a primer on the 3 ′ side of base number 493 of SEQ ID NO: 6;
(7) a primer on the 5 ′ side of base number 213 of SEQ ID NO: 7 and a primer on the 3 ′ side of base number 378 of SEQ ID NO: 7;
(8) a primer on the 5 ′ side of base number 88 of SEQ ID NO: 8 and a primer on the 3 ′ side of base number 381 of SEQ ID NO: 8;
(9) a primer on the 5 ′ side of base number 66 of SEQ ID NO: 9 and a primer on the 3 ′ side of base number 262 of SEQ ID NO: 9;
(10) a primer on the 5 ′ side of base number 163 of SEQ ID NO: 10 and a primer on the 3 ′ side of base number 356 of SEQ ID NO: 10;
(11) a primer on the 5 ′ side of base number 69 of SEQ ID NO: 11 and a primer on the 3 ′ side of base number 429 of SEQ ID NO: 11;
(12) a primer on the 5 ′ side of base number 104 of SEQ ID NO: 12 and a primer on the 3 ′ side of base number 343 of SEQ ID NO: 12;
(13) a primer on the 5 ′ side of base number 126 of SEQ ID NO: 13 and a primer on the 3 ′ side of base number 264 of SEQ ID NO: 13;
(14) a primer on the 5 ′ side of base number 132 of SEQ ID NO: 14 and a primer on the 3 ′ side of base number 189 of SEQ ID NO: 14;
(15) a primer on the 5 ′ side of base number 101 of SEQ ID NO: 15 and a primer on the 3 ′ side of base number 338 of SEQ ID NO: 15;
(16) a primer on the 5 ′ side of base number 134 of SEQ ID NO: 16 and a primer on the 3 ′ side of base number 332 of SEQ ID NO: 16;
(17) a primer on the 5 ′ side of base number 65 of SEQ ID NO: 17 and a primer on the 3 ′ side of base number 474 of SEQ ID NO: 17;
(18) a primer on the 5 ′ side of base number 96 of SEQ ID NO: 18 and a primer on the 3 ′ side of base number 133 of SEQ ID NO: 18;
(19) a primer on the 5 ′ side of base number 261 of SEQ ID NO: 19 and a primer on the 3 ′ side of base number 440 of SEQ ID NO: 19;
(20) a primer on the 5 ′ side of base number 247 of SEQ ID NO: 20 and a primer on the 3 ′ side of base number 362 of SEQ ID NO: 20;
(21) a primer on the 5 ′ side of base number 82 in SEQ ID NO: 21 and a primer on the 3 ′ side of base number 140 in SEQ ID NO: 21;
(22) a primer on the 5 ′ side of base number 359 of SEQ ID NO: 22 and a primer on the 3 ′ side of base number 364 of SEQ ID NO: 22;
(23) a primer on the 5 ′ side of base number 420 of SEQ ID NO: 23 and a primer on the 3 ′ side of base number 485 of SEQ ID NO: 23;
(24) a primer on the 5 ′ side of base number 189 of SEQ ID NO: 24 and a primer on the 3 ′ side of base number 597 of SEQ ID NO: 24;
(25) a primer on the 5 ′ side of base number 62 of SEQ ID NO: 25 and a primer on the 3 ′ side of base number 273 of SEQ ID NO: 25;
(26) a primer on the 5 ′ side of base number 57 of SEQ ID NO: 26 and a primer on the 3 ′ side of base number 434 of SEQ ID NO: 26;
(27) a primer on the 5 ′ side of base number 80 of SEQ ID NO: 27 and a primer on the 3 ′ side of base number 389 of SEQ ID NO: 27;
(28) a primer on the 5 ′ side of base number 83 of SEQ ID NO: 28 and a primer on the 3 ′ side of base number 232 of SEQ ID NO: 28;
(29) a primer on the 5 ′ side of base number 309 of SEQ ID NO: 29 and a primer on the 3 ′ side of base number 309 of SEQ ID NO: 29;
(30) a primer on the 5 ′ side of base number 216 of SEQ ID NO: 30 and a primer on the 3 ′ side of base number 216 of SEQ ID NO: 30;
(31) a primer on the 5 ′ side of base number 162 of SEQ ID NO: 31 and a primer on the 3 ′ side of base number 162 of SEQ ID NO: 31;
(32) a primer on the 5 ′ side of base number 34 of SEQ ID NO: 32 and a primer on the 3 ′ side of base number 336 of SEQ ID NO: 32;
(33) A primer on the 5 ′ side of base number 361 of SEQ ID NO: 33 and a primer on the 3 ′ side of base number 361 of SEQ ID NO: 33 The primer set according to claim 2, wherein the pair of primer sets comprises at least one combination of the following combinations (1) to (33):
(1) A combination of SEQ ID NO: 34 and SEQ ID NO: 35;
(2) a combination of SEQ ID NO: 36 and SEQ ID NO: 37;
(3) a combination of SEQ ID NO: 38 and SEQ ID NO: 39;
(4) a combination of SEQ ID NO: 40 and SEQ ID NO: 41;
(5) a combination of SEQ ID NO: 42 and SEQ ID NO: 43;
(6) a combination of SEQ ID NO: 44 and SEQ ID NO: 45;
(7) a combination of SEQ ID NO: 46 and SEQ ID NO: 47;
(8) A combination of SEQ ID NO: 48 and SEQ ID NO: 49;
(9) Combination of SEQ ID NO: 50 and SEQ ID NO: 51;
(10) A combination of SEQ ID NO: 52 and SEQ ID NO: 53;
(11) A combination of SEQ ID NO: 54 and SEQ ID NO: 55;
(12) A combination of SEQ ID NO: 56 and SEQ ID NO: 57;
(13) A combination of SEQ ID NO: 58 and SEQ ID NO: 59;
(14) a combination of SEQ ID NO: 60 and SEQ ID NO: 61;
(15) a combination of SEQ ID NO: 62 and SEQ ID NO: 63;
(16) A combination of SEQ ID NO: 64 and SEQ ID NO: 65;
(17) A combination of SEQ ID NO: 66 and SEQ ID NO: 67;
(18) A combination of SEQ ID NO: 68 and SEQ ID NO: 69;
(19) A combination of SEQ ID NO: 70 and SEQ ID NO: 71;
(20) a combination of SEQ ID NO: 72 and SEQ ID NO: 73;
(21) A combination of SEQ ID NO: 74 and SEQ ID NO: 75;
(22) a combination of SEQ ID NO: 76 and SEQ ID NO: 77;
(23) A combination of SEQ ID NO: 78 and SEQ ID NO: 79;
(24) A combination of SEQ ID NO: 80 and SEQ ID NO: 81;
(25) a combination of SEQ ID NO: 82 and SEQ ID NO: 83;
(26) a combination of SEQ ID NO: 84 and SEQ ID NO: 85;
(27) A combination of SEQ ID NO: 86 and SEQ ID NO: 87;
(28) A combination of SEQ ID NO: 88 and SEQ ID NO: 89;
(29) a combination of SEQ ID NO: 90 and SEQ ID NO: 91;
(30) a combination of SEQ ID NO: 92 and SEQ ID NO: 93;
(31) A combination of SEQ ID NO: 94 and SEQ ID NO: 95;
(32) a combination of SEQ ID NO: 96 and SEQ ID NO: 97;
(33) A combination of SEQ ID NO: 98 and SEQ ID NO: 99. The primer set according to claim 1, wherein the single nucleotide polymorphism site is at least one of the following 12 single nucleotide polymorphism sites (1) (a) to (3) (a):
(1) From SEQ ID NO: 17, (A) to (E):
(A) a single nucleotide polymorphism which is base A or T present at the position of base number 65,
(A) a single nucleotide polymorphism which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphism which is the base G or T present at the position of the base number 236,
(D) a single nucleotide polymorphism that is the base A or G existing at the position of the base number 256, and (e) a single nucleotide polymorphism that is the base C or T that exists at the position of the base number 474;
(2) SEQ ID NO: 1, (A) to (E):
(A) a single nucleotide polymorphism which is the base C or T present at the position of base number 53;
(A) a single nucleotide polymorphism in which the base C or T present at the position of base number 170 is
(C) a single nucleotide polymorphism which is base A or G present at the position of base number 185,
(D) a single nucleotide polymorphism that is the base G or T existing at the position of the base number 239, and (e) a single nucleotide polymorphism that is the base A or G that exists at the position of the base number 298;
(3) (A) and (A) of SEQ ID NO: 2:
(A) A single nucleotide polymorphism that is the base C or T existing at the position of the base number 104, and (b) a single nucleotide polymorphism that is the base C or T that exists at the position of the base number 321. The primer set according to claim 4, wherein the pair of primer sets comprises at least one combination of the following combinations (1) to (3):
(1) a primer on the 5 ′ side of base number 65 of SEQ ID NO: 17 and a primer on the 3 ′ side of base number 474 of SEQ ID NO: 17;
(2) a primer on the 5 ′ side of base number 53 of SEQ ID NO: 1 and a primer on the 3 ′ side of base number 298 of SEQ ID NO: 1;
(3) A primer on the 5 ′ side of base number 104 of SEQ ID NO: 2 and a primer on the 3 ′ side of base number 321 of SEQ ID NO: 2. The primer set according to claim 5, wherein the pair of primer sets comprises at least one combination of the following combinations (1) to (3):
(1) A combination of SEQ ID NO: 66 and SEQ ID NO: 67;
(2) a combination of SEQ ID NO: 34 and SEQ ID NO: 35;
(3) A combination of SEQ ID NO: 36 and SEQ ID NO: 37. An oligonucleotide consisting of a DNA fragment containing a single nucleotide polymorphic site on yeast genomic DNA or a label thereof, and 148 single nucleotide polymorphic sites from the following (1) (a) to (33) (a) At least one of the following single nucleotide polymorphism sites (1) (a) to (33) (a) of the nucleotide sequences of SEQ ID NOs: 1 to 33: An oligonucleotide consisting of a partial sequence consisting of 10 to 30 bases containing one polymorphic site or a sequence complementary to the partial sequence, or a label thereof:
(1) From SEQ ID NO: 1 (A) to (G):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 53;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 140;
(C) a single nucleotide polymorphic site where base C or T is at the position of base number 170,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 185,
(E) a single nucleotide polymorphic site that is base A or G present at the position of base number 225;
(F) a single nucleotide polymorphic site that is base G or T present at the position of base number 239, and (g) a single nucleotide polymorphic site that is base A or G present at the position of base number 298;
(2) SEQ ID NO: 2, (A) to (E):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 104;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 304,
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 321; and (e) a single nucleotide polymorphism site that is base A or G at the position of base number 322;
(3) (A) to (C) of SEQ ID NO: 3:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 125;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 145;
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 3 bases exists at positions of base numbers 351 to 354;
(4) SEQ ID NO: 4, (A) to (C):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 67;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 74;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 160;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 268,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 324;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 446;
(G) a single nucleotide polymorphism site that is base G or T present at the position of base number 453, and (co) a single nucleotide polymorphism site that is base A or G present at the position of base number 466;
(5) (A) and (I) of SEQ ID NO: 5:
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 431, and (a) a single nucleotide polymorphic site that is base C or T present at the position of base number 464;
(6) SEQ ID NO: 6, (A) to (S):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 75;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 185,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 216,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 217;
(E) a single nucleotide polymorphic site which is base A or C present at the position of base number 253,
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 269;
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 318,
(H) a single nucleotide polymorphic site that is base A or T present at the position of base number 441;
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 444;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 445;
(Sa) a single nucleotide polymorphic site that is base C or T present at the position of base number 463, and (f) a single nucleotide polymorphic site that is base C or T present at the position of base number 493;
(7) From SEQ ID NO: 7, (a) to (f):
(A) a single nucleotide polymorphic site that is base A or G present at the position of base number 213,
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 243;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 290,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 348;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 350, and (f) a single nucleotide polymorphic site which is base C or T present at the position of base number 378;
(8) (A) to (E) of SEQ ID NO: 8:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 88;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 152;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 161;
(D) a single nucleotide polymorphism site that is base A or G present at the position of base number 309, and (e) a single nucleotide polymorphism site that is base C or T at the position of base number 381;
(9) From SEQ ID NO: 9, (a) to (c):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 66;
(A) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 229, and (c) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 262;
(10) (A) to (C) of SEQ ID NO: 10:
(A) a single nucleotide polymorphic site which is a deletion or non-deletion of 1 base T existing at positions of base numbers 163 to 170;
(A) a single nucleotide polymorphic site that is a deletion or non-deletion of one base A present at positions of base numbers 309 to 317, and (c) one of bases A or C present at the position of base number 356 Nucleotide polymorphism site;
(11) From SEQ ID NO: 11, (A) to (G):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 69;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 93;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 111;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 208;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 210;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 408, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 429;
(12) From SEQ ID NO: 12, (a) to (c):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 104;
(A) a single nucleotide polymorphic site that is base C or G present at the position of base number 151, and (c) a single nucleotide polymorphic site that is base C or T present at the position of base number 343;
(13) (A) to (C) of SEQ ID NO: 13:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 126;
(A) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 201, and (c) a single nucleotide polymorphic site which is the base A or G present at the position of the base number 264;
(14) (A) to (C) of SEQ ID NO: 14:
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 132;
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 185, and (c) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(15) From SEQ ID NO: 15, (a) to (e):
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 101;
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(C) a single nucleotide polymorphism site in which insertion or non-insertion of 1 base A exists at positions of base numbers 188 to 192,
(D) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 280, and (e) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 338;
(16) (A) to (SA) of SEQ ID NO: 16:
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 134;
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 135;
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 137,
(D) a single nucleotide polymorphic site which is base A or T present at the position of base number 138;
(E) a single nucleotide polymorphic site which is base A or G present at the position of base number 142;
(F) a single nucleotide polymorphic site which is base A or G present at the position of base number 165,
(G) a single nucleotide polymorphic site which is base C or T present at the position of base number 183,
(H) a single nucleotide polymorphic site which is base A or C present at the position of base number 219;
(G) a single nucleotide polymorphic site which is base C or G present at the position of base number 232;
(Co) a single nucleotide polymorphic site which is base A or G present at the position of base number 293, and (sa) a single nucleotide polymorphic site which is base C or T present at the position of base number 332;
(17) From SEQ ID NO: 17, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or T present at the position of base number 65;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphic site which is base G or T present at the position of base number 236,
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 256, and (e) a single nucleotide polymorphic site which is base C or T present at the position of base number 474;
(18) (A) and (I) of SEQ ID NO: 18:
(A) a single nucleotide polymorphism site which is the base C or T present at the position of the base number 96, and (a) a single nucleotide polymorphism site which is the base C or T which is present at the position of the base number 133;
(19) (A) to (D) of SEQ ID NO: 19:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 261,
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 282;
(C) a single nucleotide polymorphic site that is base C or T present at the position of base number 371, and (d) a single nucleotide polymorphic site that is base C or T present at the position of base number 440;
(20) (A) and (A) of SEQ ID NO: 20:
(A) a single nucleotide polymorphism site that is base A or G present at the position of base number 247, and (b) a single nucleotide polymorphism site that is base C or T present at the position of base number 362;
(21) From SEQ ID NO: 21, (a) to (d):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 82;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 91;
(C) a single nucleotide polymorphic site which is the base G or T present at the position of the base number 139, and (d) a single nucleotide polymorphic site which is the base C or T present at the position of the base number 140;
(22) (a) a single nucleotide polymorphism site which is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) (A) and (I) of SEQ ID NO: 23:
(A) a single nucleotide polymorphic site which is the base A or T present at the position of the base number 420, and (a) a single nucleotide polymorphic site which is the base C or G present at the position of the base number 485;
(24) (A) to (C) of SEQ ID NO: 24:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 189;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 209,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 218,
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 260;
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 283,
(F) a single nucleotide polymorphic site which is base C or G present at the position of base number 315,
(G) a single nucleotide polymorphic site which is base A or G present at the position of base number 379;
(H) a single nucleotide polymorphic site which is base C or T present at the position of base number 385,
(G) a single nucleotide polymorphic site which is base A or C present at the position of base number 551, and (co) a single nucleotide polymorphic site which is base C or T present at the position of base number 597;
(25) (A) to (G) of SEQ ID NO: 25:
(A) a single nucleotide polymorphic site which is base C or T present at the position of base number 62;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 96;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 171;
(D) a single nucleotide polymorphic site which is base C or T present at the position of base number 176,
(E) a single nucleotide polymorphic site which is base C or T present at the position of base number 222;
(F) a single nucleotide polymorphic site which is base C or T present at the position of base number 228, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 273;
(26) From SEQ ID NO: 26, (a) to (g):
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 57;
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 149;
(C) a single nucleotide polymorphic site which is base A or T present at the position of base number 221;
(D) a single nucleotide polymorphic site which is base A or G present at the position of base number 303;
(E) a single nucleotide polymorphic site which is base A or T present at the position of base number 350;
(F) a single nucleotide polymorphic site which is base A or T present at the position of base number 400, and (g) a single nucleotide polymorphic site which is base A or G present at the position of base number 434;
(27) (A) to (E) of SEQ ID NO: 27:
(A) a single nucleotide polymorphic site which is base A or G present at the position of base number 80;
(A) a single nucleotide polymorphism site which is a deletion or non-deletion of base A existing at positions from base numbers 116 to 122;
(C) a single nucleotide polymorphic site which is base A or G present at the position of base number 204;
(D) a single nucleotide polymorphism site that is base C or T present at the position of base number 249, and (e) a single nucleotide polymorphism site that is base G or T at the position of base number 389;
(28) From SEQ ID NO: 28, (a) to (e):
(A) a single nucleotide polymorphic site which is base A or C present at the position of base number 83;
(A) a single nucleotide polymorphic site which is base C or G present at the position of base number 169,
(C) a single nucleotide polymorphic site which is base C or T present at the position of base number 193,
(D) a single nucleotide polymorphic site which is base A or C present at the position of base number 215, and (e) a single nucleotide polymorphic site which is base A or G present at the position of base number 232;
(29) (a) a single nucleotide polymorphic site which is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) a single nucleotide polymorphic site which is base C or T present at the position of base number 162 of SEQ ID NO: 31;
(32) (A) and (I) of SEQ ID NO: 32:
(A) a single nucleotide polymorphic site that is base C or T present at the position of base number 34, and (a) a single nucleotide polymorphic site that is base A or G present at the position of base number 336;
(33) (a) A single nucleotide polymorphic site which is base A or G present at the position of base number 361 in SEQ ID NO: 33. The oligonucleotide or label thereof according to claim 7, wherein the single nucleotide polymorphism site is at least one of the following 12 single nucleotide polymorphism sites (1) (a) to (3) (a):
(1) From SEQ ID NO: 17, (A) to (E):
(A) a single nucleotide polymorphism which is base A or T present at the position of base number 65,
(A) a single nucleotide polymorphism which is base A or G present at the position of base number 158,
(C) a single nucleotide polymorphism which is the base G or T present at the position of the base number 236,
(D) a single nucleotide polymorphism that is the base A or G existing at the position of the base number 256, and (e) a single nucleotide polymorphism that is the base C or T that exists at the position of the base number 474;
(2) SEQ ID NO: 1, (A) to (E):
(A) a single nucleotide polymorphism which is the base C or T present at the position of base number 53;
(A) a single nucleotide polymorphism in which the base C or T present at the position of base number 170 is
(C) a single nucleotide polymorphism which is base A or G present at the position of base number 185,
(D) a single nucleotide polymorphism that is the base G or T existing at the position of the base number 239, and (e) a single nucleotide polymorphism that is the base A or G that exists at the position of the base number 298;
(3) (A) and (A) of SEQ ID NO: 2:
(A) A single nucleotide polymorphism that is the base C or T existing at the position of the base number 104, and (b) a single nucleotide polymorphism that is the base C or T that exists at the position of the base number 321.   The oligonucleotide or label thereof according to claim 7 or 8, which is used as a probe.   An immobilized substrate on which the oligonucleotide according to claim 7 or 8 or a label thereof is immobilized.   A yeast strain identification kit comprising the primer set according to any one of claims 1 to 3, the oligonucleotide according to claim 7 or a label thereof, or the immobilized substrate according to claim 10.   A yeast strain classification kit comprising the primer set according to any one of claims 1 to 3, the oligonucleotide according to claim 7 or a label thereof, or the immobilized substrate according to claim 10.   A yeast selection kit comprising the primer set according to any one of claims 4 to 6, the oligonucleotide according to claim 8 or a label thereof, or the immobilized substrate according to claim 10. For yeast genomic DNA, alleles consisting of a combination selected from (1) to (33) below or a plurality selected from (1) to (33) are detected, and yeast strains are identified based on the results. A method for identifying a strain of yeast characterized by:
(1) At least one of (a) to (ki) of SEQ ID NO: 1:
(A) an allele which is a base C or T present at the position of base number 53,
(A) an allele that is base A or G present at the position of base number 140;
(C) an allele whose base C or T is at the position of base number 170,
(D) an allele that is base A or G present at the position of base number 185,
(E) an allele that is base A or G present at the position of base number 225,
(F) an allele which is the base G or T present at the position of the base number 239, and (g) an allele which is the base A or G present at the position of the base number 298;
(2) at least one of (a) to (e) of SEQ ID NO: 2:
(A) an allele which is the base C or T present at the position of the base number 104;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base C or T present at the position of base number 304,
(D) an allele which is the base C or T present at the position of the base number 321 and (e) an allele which is the base A or G present at the position of the base number 322;
(3) at least one of (a) to (c) of SEQ ID NO: 3:
(A) an allele which is a base C or T present at the position of base number 125,
(A) an allele that is base A or G present at the position of base number 145;
(C) an allele with insertion or non-insertion of 3 bases present at positions of base numbers 351 to 354;
(4) at least one of (a) to (co) of SEQ ID NO: 4:
(A) an allele which is a base C or T present at the position of base number 67,
(A) an allele which is a base C or T present at the position of base number 74;
(C) an allele that is base A or G present at the position of base number 104;
(D) an allele which is a base C or T present at the position of base number 160;
(E) an allele that is base A or C present at the position of base number 268,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base A or G present at the position of base number 324;
(H) an allele that is base C or T present at the position of base number 446;
(G) an allele which is the base G or T present at the position of the base number 453, and (co) an allele which is the base A or G present at the position of the base number 466;
(5) At least one of (a) and (b) of SEQ ID NO: 5:
(A) an allele which is the base A or G present at the position of the base number 431, and (b) an allele which is the base C or T present at the position of the base number 464;
(6) At least one of (A) to (B) of SEQ ID NO: 6:
(A) an allele that is base A or C present at the position of base number 75;
(A) an allele which is the base C or T present at the position of the base number 185,
(C) an allele which is the base C or T present at the position of the base number 216,
(D) an allele that is base A or C present at the position of base number 217,
(E) an allele that is base A or C present at the position of base number 253,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base C or T present at the position of base number 318,
(H) an allele that is base A or T present at the position of base number 441;
(G) an allele that is base A or G present at the position of base number 444;
(E) an allele that is base A or T present at the position of base number 445;
(Sa) an allele that is a base C or T present at the position of base number 463, and (f) an allele that is a base C or T present at the position of base number 493;
(7) At least one of (a) to (f) of SEQ ID NO: 7:
(A) an allele that is base A or G present at the position of base number 213,
(A) an allele that is base A or G present at the position of base number 243;
(C) an allele that is base C or T present at the position of base number 290,
(D) an allele that is base A or T present at the position of base number 348;
(E) an allele which is the base A or G present at the position of the base number 350, and (f) an allele which is the base C or T present at the position of the base number 378;
(8) at least one of (A) to (E) of SEQ ID NO: 8:
(A) an allele that is base A or G present at the position of base number 88;
(A) an allele which is a base C or T present at the position of base number 152;
(C) an allele that is base C or T present at the position of base number 161;
(D) an allele which is the base A or G present at the position of the base number 309, and (e) an allele which is the base C or T present at the position of the base number 381;
(9) At least one of (A) to (U) of SEQ ID NO: 9:
(A) an allele which is a base C or T present at the position of base number 66;
(A) an allele that is the base C or T present at the position of the base number 229, and (c) an allele that is the base A or T at the position of the base number 262;
(10) At least one of (a) to (c) of SEQ ID NO: 10:
(A) an allele that is a deletion or non-deletion of 1 base T present at positions from base numbers 163 to 170;
(A) an allele that is deleted or non-deletion of 1 base A present at the position of base numbers 309 to 317, and (c) an allele that is base A or C at the position of base number 356;
(11) At least one of (a) to (ki) of SEQ ID NO: 11:
(A) an allele that is base A or T present at the position of base number 69;
(A) an allele which is a base C or T present at the position of base number 93;
(C) an allele that is base A or G present at the position of base number 111;
(D) an allele which is the base C or T present at the position of the base number 208;
(E) an allele that is base A or G present at the position of base number 210;
(F) an allele which is the base C or T present at the position of the base number 408, and (g) an allele which is the base A or G present at the position of the base number 429;
(12) At least one of (a) to (c) of SEQ ID NO: 12:
(A) an allele that is base A or G present at the position of base number 104;
(A) an allele that is the base C or G present at the position of the base number 151, and (c) an allele that is the base C or T present at the position of the base number 343;
(13) at least one of (a) to (c) of SEQ ID NO: 13:
(A) an allele that is base A or G present at the position of base number 126;
(A) an allele that is the base C or G present at the position of the base number 201, and (c) an allele that is the base A or G present at the position of the base number 264;
(14) At least one of (A) to (U) of SEQ ID NO: 14:
(A) an allele that is base A or C present at the position of base number 132;
(A) an allele that is base A or C present at the position of base number 185, and (c) an allele that is base C or T present at the position of base number 189;
(15) at least one of (A) to (E) of SEQ ID NO: 15:
(A) an allele that is base C or T present at the position of base number 101,
(A) an allele that is base C or T present at the position of base number 183,
(C) an allele that is inserted or non-inserted into 1 base A existing at positions of base numbers 188 to 192,
(D) an allele which is the base C or T present at the position of the base number 280, and (e) an allele which is the base G or T present at the position of the base number 338;
(16) At least one of (a) to (sa) of SEQ ID NO: 16:
(A) an allele that is base A or T present at the position of base number 134;
(A) an allele that is base A or T present at the position of base number 135;
(C) an allele that is base C or T present at the position of base number 137,
(D) an allele that is base A or T present at the position of base number 138;
(E) an allele that is base A or G present at the position of base number 142;
(F) an allele that is base A or G present at the position of base number 165,
(G) an allele that is base C or T present at the position of base number 183,
(H) an allele that is base A or C present at the position of base number 219,
(G) an allele that is base C or G present at the position of base number 232;
(Co) an allele that is base A or G at the position of base number 293, and (sa) an allele that is base C or T at the position of base number 332;
(17) at least one of (a) to (e) of SEQ ID NO: 17:
(A) an allele that is base A or T present at the position of base number 65,
(A) an allele that is base A or G present at the position of base number 158,
(C) an allele that is the base G or T at the position of base number 236,
(D) an allele that is base A or G present at the position of base number 256, and (e) an allele that is base C or T at the position of base number 474;
(18) at least one of (a) and (b) of SEQ ID NO: 18:
(A) an allele which is the base C or T present at the position of the base number 96, and (a) an allele which is the base C or T present at the position of the base number 133;
(19) At least one of (a) to (e) of SEQ ID NO: 19:
(A) an allele that is base A or G present at the position of base number 261,
(A) an allele that is base C or T present at the position of base number 282,
(C) an allele that is base C or T present at the position of base number 371, and (d) an allele that is base C or T at the position of base number 440;
(20) At least one of (a) and (b) of SEQ ID NO: 20:
(A) an allele which is the base A or G present at the position of the base number 247, and (b) an allele which is the base C or T present at the position of the base number 362;
(21) at least one of (a) to (e) of SEQ ID NO: 21:
(A) an allele that is base A or G present at the position of base number 82;
(A) an allele that is base A or G present at the position of base number 91;
(C) an allele that is the base G or T present at the position of the base number 139, and (d) an allele that is the base C or T at the position of the base number 140;
(22) (a) an allele that is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) at least one of (a) and (b) of SEQ ID NO: 23:
(A) an allele which is the base A or T present at the position of the base number 420, and (b) an allele which is the base C or G present at the position of the base number 485;
(24) at least one of (a) to (co) of SEQ ID NO: 24:
(A) an allele which is the base C or T present at the position of base number 189,
(A) an allele that is base A or G present at the position of base number 209;
(C) an allele that is base C or T present at the position of base number 218,
(D) an allele that is base C or T present at the position of base number 260;
(E) an allele that is base C or T present at the position of base number 283,
(F) an allele that is base C or G present at the position of base number 315,
(G) an allele that is base A or G present at the position of base number 379;
(H) an allele which is the base C or T present at the position of the base number 385,
(G) an allele which is the base A or C present at the position of the base number 551, and (co) an allele which is the base C or T present at the position of the base number 597;
(25) at least one of (a) to (ki) of SEQ ID NO: 25:
(A) an allele which is a base C or T present at the position of base number 62;
(A) an allele that is base A or G present at the position of base number 96;
(C) an allele that is base A or G present at the position of base number 171;
(D) an allele that is base C or T present at the position of base number 176,
(E) an allele that is base C or T present at the position of base number 222;
(F) an allele which is the base C or T present at the position of the base number 228, and (g) an allele which is the base A or G present at the position of the base number 273;
(26) At least one of (a) to (ki) of SEQ ID NO: 26:
(A) an allele that is base A or G present at the position of base number 57;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base A or T present at the position of base number 221;
(D) an allele that is base A or G present at the position of base number 303;
(E) an allele that is base A or T present at the position of base number 350;
(F) an allele that is base A or T present at the position of base number 400, and (g) an allele that is base A or G that is present at the position of base number 434;
(27) At least one of (a) to (e) in SEQ ID NO: 27:
(A) an allele that is base A or G present at the position of base number 80;
(A) an allele that is a deletion or non-deletion of base A present at positions from base numbers 116 to 122;
(C) an allele that is base A or G present at the position of base number 204;
(D) an allele that is base C or T present at the position of base number 249, and (e) an allele that is base G or T at the position of base number 389;
(28) At least one of (a) to (e) of SEQ ID NO: 28:
(A) an allele that is base A or C present at the position of base number 83;
(A) an allele which is a base C or G present at the position of base number 169,
(C) an allele that is base C or T present at the position of base number 193,
(D) an allele that is the base A or C existing at the position of the base number 215, and (e) an allele that is the base A or G that exists at the position of the base number 232;
(29) (a) an allele that is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) an allele which is the base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) An allele of base C or T present in the position of base number 162 of SEQ ID NO: 31;
(32) At least one of (a) and (b) of SEQ ID NO: 32:
(A) an allele that is the base C or T present at the position of the base number 34, and (a) an allele that is the base A or G that is present at the position of the base number 336;
(33) (a) An allele of base A or G present at the position of base number 361 of SEQ ID NO: 33.   The method according to claim 14, wherein the allele is detected using the primer set according to any one of claims 1 to 3, the probe according to claim 7, or the immobilized substrate according to claim 10. For the genomic DNA of yeast, an allele comprising any combination selected from (1) to (33) below or a plurality selected from (1) to (33) is detected, and based on the results, a plurality of yeast strains are detected. A method for classifying yeast strains, characterized by determining an affinity relationship:
(1) At least one of (a) to (ki) of SEQ ID NO: 1:
(A) an allele which is a base C or T present at the position of base number 53,
(A) an allele that is base A or G present at the position of base number 140;
(C) an allele whose base C or T is at the position of base number 170,
(D) an allele that is base A or G present at the position of base number 185,
(E) an allele that is base A or G present at the position of base number 225,
(F) an allele which is the base G or T present at the position of the base number 239, and (g) an allele which is the base A or G present at the position of the base number 298;
(2) at least one of (a) to (e) of SEQ ID NO: 2:
(A) an allele which is the base C or T present at the position of the base number 104;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base C or T present at the position of base number 304,
(D) an allele which is the base C or T present at the position of the base number 321 and (e) an allele which is the base A or G present at the position of the base number 322;
(3) at least one of (a) to (c) of SEQ ID NO: 3:
(A) an allele which is a base C or T present at the position of base number 125,
(A) an allele that is base A or G present at the position of base number 145;
(C) an allele with insertion or non-insertion of 3 bases present at positions of base numbers 351 to 354;
(4) at least one of (a) to (co) of SEQ ID NO: 4:
(A) an allele which is a base C or T present at the position of base number 67,
(A) an allele which is a base C or T present at the position of base number 74;
(C) an allele that is base A or G present at the position of base number 104;
(D) an allele which is a base C or T present at the position of base number 160;
(E) an allele that is base A or C present at the position of base number 268,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base A or G present at the position of base number 324;
(H) an allele that is base C or T present at the position of base number 446;
(G) an allele which is the base G or T present at the position of the base number 453, and (co) an allele which is the base A or G present at the position of the base number 466;
(5) At least one of (a) and (b) of SEQ ID NO: 5:
(A) an allele which is the base A or G present at the position of the base number 431, and (b) an allele which is the base C or T present at the position of the base number 464;
(6) At least one of (A) to (B) of SEQ ID NO: 6:
(A) an allele that is base A or C present at the position of base number 75;
(A) an allele which is the base C or T present at the position of the base number 185,
(C) an allele which is the base C or T present at the position of the base number 216,
(D) an allele that is base A or C present at the position of base number 217,
(E) an allele that is base A or C present at the position of base number 253,
(F) an allele that is base C or T present at the position of base number 269;
(G) an allele that is base C or T present at the position of base number 318,
(H) an allele that is base A or T present at the position of base number 441;
(G) an allele that is base A or G present at the position of base number 444;
(E) an allele that is base A or T present at the position of base number 445;
(Sa) an allele that is a base C or T present at the position of base number 463, and (f) an allele that is a base C or T present at the position of base number 493;
(7) At least one of (a) to (f) of SEQ ID NO: 7:
(A) an allele that is base A or G present at the position of base number 213,
(A) an allele that is base A or G present at the position of base number 243;
(C) an allele that is base C or T present at the position of base number 290,
(D) an allele that is base A or T present at the position of base number 348;
(E) an allele which is the base A or G present at the position of the base number 350, and (f) an allele which is the base C or T present at the position of the base number 378;
(8) at least one of (A) to (E) of SEQ ID NO: 8:
(A) an allele that is base A or G present at the position of base number 88;
(A) an allele which is a base C or T present at the position of base number 152;
(C) an allele that is base C or T present at the position of base number 161;
(D) an allele which is the base A or G present at the position of the base number 309, and (e) an allele which is the base C or T present at the position of the base number 381;
(9) At least one of (A) to (U) of SEQ ID NO: 9:
(A) an allele which is a base C or T present at the position of base number 66;
(A) an allele that is the base C or T present at the position of the base number 229, and (c) an allele that is the base A or T at the position of the base number 262;
(10) At least one of (a) to (c) of SEQ ID NO: 10:
(A) an allele that is a deletion or non-deletion of 1 base T present at positions from base numbers 163 to 170;
(A) an allele that is deleted or non-deletion of 1 base A present at the position of base numbers 309 to 317, and (c) an allele that is base A or C at the position of base number 356;
(11) At least one of (a) to (ki) of SEQ ID NO: 11:
(A) an allele that is base A or T present at the position of base number 69;
(A) an allele which is a base C or T present at the position of base number 93;
(C) an allele that is base A or G present at the position of base number 111;
(D) an allele which is the base C or T present at the position of the base number 208;
(E) an allele that is base A or G present at the position of base number 210;
(F) an allele which is the base C or T present at the position of the base number 408, and (g) an allele which is the base A or G present at the position of the base number 429;
(12) At least one of (a) to (c) of SEQ ID NO: 12:
(A) an allele that is base A or G present at the position of base number 104;
(A) an allele that is the base C or G present at the position of the base number 151, and (c) an allele that is the base C or T present at the position of the base number 343;
(13) at least one of (a) to (c) of SEQ ID NO: 13:
(A) an allele that is base A or G present at the position of base number 126;
(A) an allele that is the base C or G present at the position of the base number 201, and (c) an allele that is the base A or G present at the position of the base number 264;
(14) At least one of (A) to (U) of SEQ ID NO: 14:
(A) an allele that is base A or C present at the position of base number 132;
(A) an allele that is base A or C present at the position of base number 185, and (c) an allele that is base C or T present at the position of base number 189;
(15) at least one of (A) to (E) of SEQ ID NO: 15:
(A) an allele that is base C or T present at the position of base number 101,
(A) an allele that is base C or T present at the position of base number 183,
(C) an allele that is inserted or non-inserted into 1 base A existing at positions of base numbers 188 to 192,
(D) an allele which is the base C or T present at the position of the base number 280, and (e) an allele which is the base G or T present at the position of the base number 338;
(16) At least one of (a) to (sa) of SEQ ID NO: 16:
(A) an allele that is base A or T present at the position of base number 134;
(A) an allele that is base A or T present at the position of base number 135;
(C) an allele that is base C or T present at the position of base number 137,
(D) an allele that is base A or T present at the position of base number 138;
(E) an allele that is base A or G present at the position of base number 142;
(F) an allele that is base A or G present at the position of base number 165,
(G) an allele that is base C or T present at the position of base number 183,
(H) an allele that is base A or C present at the position of base number 219,
(G) an allele that is base C or G present at the position of base number 232;
(Co) an allele that is base A or G at the position of base number 293, and (sa) an allele that is base C or T at the position of base number 332;
(17) at least one of (a) to (e) of SEQ ID NO: 17:
(A) an allele that is base A or T present at the position of base number 65,
(A) an allele that is base A or G present at the position of base number 158,
(C) an allele that is the base G or T at the position of base number 236,
(D) an allele that is base A or G present at the position of base number 256, and (e) an allele that is base C or T at the position of base number 474;
(18) at least one of (a) and (b) of SEQ ID NO: 18:
(A) an allele which is the base C or T present at the position of the base number 96, and (a) an allele which is the base C or T present at the position of the base number 133;
(19) At least one of (a) to (e) of SEQ ID NO: 19:
(A) an allele that is base A or G present at the position of base number 261,
(A) an allele that is base C or T present at the position of base number 282,
(C) an allele that is base C or T present at the position of base number 371, and (d) an allele that is base C or T at the position of base number 440;
(20) At least one of (a) and (b) of SEQ ID NO: 20:
(A) an allele which is the base A or G present at the position of the base number 247, and (b) an allele which is the base C or T present at the position of the base number 362;
(21) at least one of (a) to (e) of SEQ ID NO: 21:
(A) an allele that is base A or G present at the position of base number 82;
(A) an allele that is base A or G present at the position of base number 91;
(C) an allele that is the base G or T present at the position of the base number 139, and (d) an allele that is the base C or T at the position of the base number 140;
(22) (a) an allele that is a deletion or non-deletion of base A present at positions of base numbers 359 to 364 of SEQ ID NO: 22;
(23) at least one of (a) and (b) of SEQ ID NO: 23:
(A) an allele which is the base A or T present at the position of the base number 420, and (b) an allele which is the base C or G present at the position of the base number 485;
(24) at least one of (a) to (co) of SEQ ID NO: 24:
(A) an allele which is the base C or T present at the position of base number 189,
(A) an allele that is base A or G present at the position of base number 209;
(C) an allele that is base C or T present at the position of base number 218,
(D) an allele that is base C or T present at the position of base number 260;
(E) an allele that is base C or T present at the position of base number 283,
(F) an allele that is base C or G present at the position of base number 315,
(G) an allele that is base A or G present at the position of base number 379;
(H) an allele which is the base C or T present at the position of the base number 385,
(G) an allele which is the base A or C present at the position of the base number 551, and (co) an allele which is the base C or T present at the position of the base number 597;
(25) at least one of (a) to (ki) of SEQ ID NO: 25:
(A) an allele which is a base C or T present at the position of base number 62;
(A) an allele that is base A or G present at the position of base number 96;
(C) an allele that is base A or G present at the position of base number 171;
(D) an allele that is base C or T present at the position of base number 176,
(E) an allele that is base C or T present at the position of base number 222;
(F) an allele which is the base C or T present at the position of the base number 228, and (g) an allele which is the base A or G present at the position of the base number 273;
(26) At least one of (a) to (ki) of SEQ ID NO: 26:
(A) an allele that is base A or G present at the position of base number 57;
(A) an allele that is base A or G present at the position of base number 149;
(C) an allele that is base A or T present at the position of base number 221;
(D) an allele that is base A or G present at the position of base number 303;
(E) an allele that is base A or T present at the position of base number 350;
(F) an allele that is base A or T present at the position of base number 400, and (g) an allele that is base A or G that is present at the position of base number 434;
(27) At least one of (a) to (e) in SEQ ID NO: 27:
(A) an allele that is base A or G present at the position of base number 80;
(A) an allele that is a deletion or non-deletion of base A present at positions from base numbers 116 to 122;
(C) an allele that is base A or G present at the position of base number 204;
(D) an allele that is base C or T present at the position of base number 249, and (e) an allele that is base G or T at the position of base number 389;
(28) At least one of (a) to (e) of SEQ ID NO: 28:
(A) an allele that is base A or C present at the position of base number 83;
(A) an allele which is a base C or G present at the position of base number 169,
(C) an allele that is base C or T present at the position of base number 193,
(D) an allele that is the base A or C existing at the position of the base number 215, and (e) an allele that is the base A or G that exists at the position of the base number 232;
(29) (a) an allele that is base A or G present at the position of base number 309 in SEQ ID NO: 29;
(30) (a) an allele which is the base C or T present at the position of base number 216 of SEQ ID NO: 30;
(31) (a) An allele of base C or T present in the position of base number 162 of SEQ ID NO: 31;
(32) At least one of (a) and (b) of SEQ ID NO: 32:
(A) an allele that is the base C or T present at the position of the base number 34, and (a) an allele that is the base A or G that is present at the position of the base number 336;
(33) (a) An allele of base A or G present at the position of base number 361 of SEQ ID NO: 33.   The method according to claim 16, wherein the allele is detected using the primer set according to any one of claims 1 to 3, the probe according to claim 7, or the immobilized substrate according to claim 10.   The method according to claim 16 or 17, characterized in that the determination of the affinity uses cluster analysis.   The method according to claim 18, wherein the algorithm of cluster analysis is UPGMA method. A method for detecting an allele comprising the combination of any of the following (1) and (2) and (3) with respect to genomic DNA of yeast and selecting the yeast based on the result:
(1) at least one of SEQ ID NO: 17 from (a) to (e):
(A) an allele that is base A or T present at the position of base number 65,
(A) an allele that is base A or G present at the position of base number 158,
(C) an allele that is the base G or T at the position of base number 236,
(D) an allele that is base A or G present at the position of base number 256, and (e) an allele that is base C or T at the position of base number 474;
(2) at least one of (A) to (E) of SEQ ID NO: 1
(A) an allele which is a base C or T present at the position of base number 53,
(A) an allele of the base C or T existing at the position of base number 170;
(C) an allele that is base A or G present at the position of base number 185,
(D) an allele which is the base G or T present at the position of the base number 239, and (e) an allele which is the base A or G present at the position of the base number 298;
(3) at least one of (a) and (b) of SEQ ID NO: 2
(A) an allele which is the base C or T present at the position of the base number 104, and (a) an allele which is the base C or T present at the position of the base number 321;   The method according to claim 20, wherein the allele is detected using the primer set according to any one of claims 4 to 6, the probe according to claim 8, or the immobilized substrate according to claim 10.   The method according to claim 20 or 21, wherein the yeast to be selected has a high sulfite producing ability as a brewing characteristic.   The method according to claim 20 or 21, wherein the selected yeast has a low sulfite producing ability as a brewing characteristic.   The method according to claim 20 or 21, wherein the selected yeast has a high hydrogen sulfide producing ability as a brewing characteristic.   The method according to claim 20 or 21, characterized in that the yeast to be selected has a low ability to produce hydrogen sulfide as a brewing characteristic.   The method according to claim 20 or 21, wherein the yeast to be selected has a high ester-producing ability as a brewing characteristic.   The method according to claim 20 or 21, characterized in that the yeast to be selected has a low ester-producing ability as a brewing characteristic.   The method according to claim 20 or 21, wherein the selected yeast has a higher alcohol-producing ability as a brewing characteristic.   The method according to claim 20 or 21, wherein the yeast to be selected has a lower higher alcohol-producing ability as a brewing characteristic.   30. The method according to any one of claims 14 to 29, wherein the yeast species is upper surface fermentation yeast or lower surface fermentation yeast.   Detection is performed by direct sequencing method, DHPLC (Denaturing High Performance Liquid Chromatography) method, method using Surveyor Nuclease, TaqMan PCR method, Invader method, primer extension method, SSCP (Single Strand Condrum method) 31. A method according to any one of claims 14 to 30, characterized in that it is carried out using one or more methods selected from the group consisting of methods.

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