JP2006314273A - Method for collecting momordicin from momordica charantia as raw material - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for collecting momordicin at high purity from Momordica charantia. <P>SOLUTION: The method for collecting momordicin comprises soaking Momordica charantia with ethanol to produce a Momordica charantia extract, adjusting the extract to be acidic, leaving the extract to stand under refrigeration, separating it into a supernatant and a precipitate, and removing the precipitate. Deposition of momordicin is performed through adding fine powder of Momordica charantia to the supernatant from which the precipitate is removed. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method for recovering momordesine using bitter melon as a raw material.

  Nowadays, the existence of various active ingredients contained in bitter melon has been clarified, and bitter-containing products in which bitter melon ingredients are incorporated by focusing on such active ingredients have been proposed in various fields such as food and cosmetics. ing.

In the above-mentioned bitter melon-containing product, an extract of bitter melon fruit, a pressed solution, and the like are mixed into the product so that the active ingredient of bitter melon is contained in the product (for example, see Patent Document 1).
Japanese Patent Laid-Open No. 2002-212050

  However, since the various extracts in the bitter melon are mixed in the bitter melon extract and the above-described squeezed solution, if the extract and the squeezed solution are used as they are, as long as they are limited to components having a specific effect. However, the content of the component itself having a specific effect due to the other components is reduced, and the effect is reduced.

  In addition, the other components may interfere with the action of the component having the specific effect.

  The inventor of the present application has focused on momordesin, which is a bitter component of bitter melon and has a therapeutic action for diabetes, and has studied a method for recovering the momordesin from bitter melon with high purity. The object of the present invention is to recover highly pure momordesin from bitter melon.

  In the method for recovering momordesine using bitter melon as a raw material of the present invention, the bitter melon is extracted with ethanol to produce a bitter melon extract solution, and the bitter melon extract solution is adjusted to acidity and then left to stand under refrigeration to obtain a supernatant and a precipitate. It was decided to remove the precipitate after separation.

  In addition, the method for recovering momordesin using the bitter melon of the present invention as a raw material is characterized in that momordesine is precipitated by adding fine powder of bitter melon to the supernatant from which the precipitate has been removed.

  In addition, in the method for recovering momordesine using bitter melon as a raw material of the present invention, the bitter melon is extracted with ethanol to produce a bitter melon extract solution, and a substance having a molecular weight of 600 or more is separated and recovered from the bitter melon extract solution using a molecular sieve. did.

In addition, the method for recovering momordesine using the bitter melon of the present invention as a raw material is also characterized by the following points.
(1) The bitter melon extract solution is separated into substances having molecular weights of 618, 632, and 816 by molecular sieve.
(2) The bitter melon extract solution is adjusted to be acidic and then allowed to stand under refrigeration to separate the supernatant liquid and the precipitate, and then the precipitate is removed.

  According to the first aspect of the present invention, the bitter melon is extracted with ethanol to produce a bitter melon extract solution, and the bitter melon extract solution is acidified and then left to stand under refrigeration to separate into a supernatant and a precipitate. After that, we decided to remove the precipitate, so we can remove the precipitate that contains a lot of low-molecular substances from the bitter melon extract solution, leaving a supernatant that contains a lot of high-molecular substances that become momordesine, and efficiently using momordesin. It can be recovered.

  According to the present invention of claim 2, since it was decided to precipitate momordesin by adding a fine powder of bitter melon to the supernatant liquid from which the precipitate was removed, it becomes easier to deposit momordesin with the fine powder as a nucleus, Momordesin can be efficiently recovered from the bitter melon extract.

  According to the third aspect of the present invention, the bitter melon is extracted with ethanol to produce a bitter melon extract solution, and a substance having a molecular weight of 600 or more is separated and recovered from the bitter melon extract solution with a molecular sieve. Can be recovered by a simple method without cost.

  According to the fourth aspect of the present invention, since the bitter melon extract solution is classified into substances having molecular weights of 618, 632, and 816 by molecular sieving, the constituents of momordesin are divided into three molecular weights. The molecular weight of the momordesine constituent material can be properly used according to the application.

  According to the fifth aspect of the present invention, the bitter melon extraction solution is adjusted to acidity and then left to stand under refrigeration, and the precipitate is removed after separation into a supernatant and a precipitate. A precipitate containing a large amount of low-molecular substances can be removed from the solution to leave a supernatant liquid containing a large amount of high-molecular substances that become momordesine, and momordesin can be efficiently recovered.

  The method for recovering momordesine using bitter melon as a raw material according to the present invention is to extract bitter melon with ethanol to produce a bitter melon extract, and recover momordesine from the bitter melon extract.

  All the species contained in the genus Cucurbitaceae and momordica can be used for the bitter melon, which is a raw material, and momordesin can be recovered particularly efficiently among the cucurbitaceae plants from the bitter melon.

  The bitter melon can be used as it is or after it has been dried, but especially when it is used as a dry powder, the area of the dry powder in contact with ethanol is increased to extract momordesine. Efficiency can be improved.

  When dried bitter melon is used, if the bitter melon is dried slowly by natural drying, secondary bonds are formed between sugar molecules in the bitter melon, preventing water from penetrating and reducing the solubility of momordesine. Therefore, it is desirable to dry the bitter melon quickly.

  The concentration of ethanol used as a solvent is desirably 70 to 85% by weight. By using ethanol in such a concentration range, the extraction efficiency of momordesin can be improved, and the concentration of momordesin as a final processed product can be improved. The water content can be suppressed and the quality can be easily maintained.

  In the method for recovering momordesine according to the present invention, the bitter melon and ethanol are mixed to produce a bitter melon extract from which the bitter melon component containing momordesine is first extracted.

  Next, ethanol is recovered from the produced bitter melon extract by vacuum concentration, etc., adjusted to acidity, and then allowed to stand under refrigeration to separate into a supernatant and a precipitate, and the precipitate is removed. The precipitate contains a large amount of low molecular weight substances having a molecular weight of 600 or less, and the supernatant liquid contains a large amount of high molecular weight substances having a molecular weight of 600 or more that can be a constituent of momordesine. Therefore, momordesine can be efficiently recovered by removing the precipitate from the bitter melon extract solution.

  Next, momordesin is precipitated by adding about 2 to 3% of the weight of the supernatant liquid to the supernatant liquid from which the precipitate has been removed. The fine powder of bitter melon becomes a nucleus when the crystals of momordesin precipitate, so that the addition of the fine powder of bitter melon facilitates the precipitation of momordesine, and the momordesine can be efficiently recovered from the bitter melon extract solution.

  The momordesin deposited as described above is mixed with ethanol and extracted again. In this way, since extraction with ethanol is performed in two steps, it is possible to recover momordesin with higher purity from bitter melon. Moreover, in the two extractions, since highly safe ethanol is used as the solvent, there is no danger even if the solvent remains in the final processed product, momordesin.

  When extracting momordesine for the second time, it is desirable to use ethanol at a concentration higher than that of the first time, with the ethanol concentration being 100% by weight. Thereby, the extraction amount of momordesine can be increased.

  Next, the momordesin-extracted solution in which momordesin is dissolved is centrifuged to separate into ethanol as a supernatant and momordesin as a precipitate.

  Alternatively, the momordesine extraction solution may be subjected to molecular sieving to recover a substance having a high momordesine content and a molecular weight of 600 or more as a solution. In particular, when recovering momordesin by molecular sieve, if the substances with molecular weights of 618, 632, and 816, which are constituents of momordesin, are separately recovered, the constituents of momordesin with different molecular weights can be used according to the application. Can do. Thus, when recovering momordesin by molecular sieve, it is necessary to recover ethanol from the solution containing momordesin permeated through the molecular sieve by vacuum concentration or the like.

  In addition, when recovering momordesin by molecular sieve, the supernatant after removing the precipitate by separating the supernatant by separating the supernatant by adjusting the pH without adding the fine powder of bitter melon directly. The bitter extract solution can be directly sifted through a molecular sieve without being separated into a supernatant and a precipitate by pH adjustment. Moreover, as a molecular sieve, the column which used the silica gel for the filler can be used suitably.

  The momordesin separated from the momordesin extraction solution by centrifugation or molecular sieve as described above is dried by spray drying or hot air drying, and sieved to select a powder of 100 mesh or less. Thus, by making the shape of momordesine into a dry powder state, it becomes easy to handle and the quality is easily maintained.

  In particular, it is desirable to perform the drying process by spray drying or hot air drying at a temperature of 60 ° C. to 140 ° C. By performing the drying process in such a temperature range, it is possible to efficiently dry without modifying momordesin. .

  It should be noted that the second momordesine extraction described above and the ethanol recovery associated therewith are omitted, and the momordesine precipitated by adding the fine powder of bitter melon after the first momordesine extraction is directly applied by spray drying or hot air drying. It can also be dried.

  Hereinafter, an embodiment of a method for recovering momordesine using bitter melon as a raw material according to the present invention will be described more specifically.

[Embodiment 1]
(1) First momordesine extract production step First, momordesin is extracted by mixing dry powder of bitter leaves, stems, and roots with 75% ethanol as a solvent.

(2) Solvent recovery step Next, the extraction solution is heated at a temperature of about 90 degrees to evaporate the solvent, and then concentrated under reduced pressure to recover the evaporated solvent at a temperature of about 80 degrees for about 5 hours. To recover most of the solvent from the extracted solution.

(3) First standing step Next, the concentrated extraction solution is allowed to stand at a temperature of 5 degrees for 24 hours, and the fat content in the extraction solution is generated as a solid content.

(4) 1st filtration process Next, the extraction solution after standing is filtered by membrane separation or celite (diatomaceous earth), and the fat and other precipitates generated in the previous process are removed from the extraction solution.

(5) Second standing step Next, the extraction solution consisting of the filtrate is adjusted to pH 4 to 5 with acetic acid, and the pH-adjusted extraction solution is allowed to stand again at a temperature of 5 degrees for 24 hours. Separate into supernatant and precipitate.

(6) Second filtration step Next, the extracted solution after standing is filtered through a filter to remove low-molecular substances such as pigments and some jelly-like impurities (phytocollage) in the extracted solution that becomes a precipitate. To do.

(7) Second momordesine extract production step Next, a fine powder having a particle size of about 20 μm of bitter leaves, stems and / or roots is added to the extract solution consisting of the filtrate for 2% of its weight. Crystals are precipitated. Thus, by adding the fine powder of bitter melon, the fine powder can be used as a seed to efficiently precipitate momordesin.

  The momordesin crystals precipitated by the above treatment are dissolved in 100% ethanol, which is a hydrophilic solvent, and momordesin is extracted again.

(8) Centrifugation process Next, the extraction solution produced | generated by the said 2nd momordesine extract production | generation process is centrifuged, and it isolate | separates into the deposit containing momordesine, and the water | moisture content containing ethanol, and removes a water | moisture content.

(9) Drying step Next, the precipitate separated by centrifugation is dried with hot air at a temperature of 85 degrees for 6 hours.

(10) Sorting Step The dried momordesine powder dried as described above is sieved to sort a substance of 100 mesh or less, and this is used as the final processed product.

  According to the method for recovering momordesin comprising the steps (1) to (10), momordesin contained in bitter melon can be recovered with high purity.

[Embodiment 2]
In the method for recovering momordesine of this embodiment, the steps of (1) to (6) are the same as those of the first embodiment, and the subsequent steps are different. Therefore, in the following, the steps (1) to (6) common to the first embodiment are omitted, and the steps after (7) characteristic of the present embodiment will be described.

(7) Column permeation step In the column permeation step, the extraction solution after completion of the second filtration step in (6) of Embodiment 1 above is passed through a column packed with silica gel, and first, purified water is used as a solvent to reduce the concentration. Wash off molecular weight sugar. Next, substances having molecular weights of 618, 632, and 816 are recovered as solutions using 65 to 70% ethanol as a solvent.

(8) Second Solvent Recovery Step Next, the solution containing each molecular weight substance is concentrated under reduced pressure for 1 hour under the same temperature conditions as in the solvent recovery step in (2) of Embodiment 1 above, and ethanol as the solvent is added. Recover from solution.

(9) Drying step Next, the residue after ethanol recovery is dried by spray drying under temperature conditions of an inlet temperature of 80 degrees and an outlet temperature of 100 to 120 degrees.

(10) Sorting step The dried powder of momordesin for each molecular weight dried as described above is screened to screen a substance of 100 mesh or less as in the sorting step in (10) of Embodiment 1, and this is finally Treated.

  According to the method for recovering momordesine of Embodiment 2 above, momordesin contained in bitter melon can be recovered with high purity, and the constituents of momordesin can be recovered by dividing them into three molecular weights. Constituent substances can be properly used according to the application.

[Embodiment 3]
In the method for recovering momordesine of this embodiment, the steps 1 and 2 and the steps (1) to (6) are common, and the subsequent steps are different. Therefore, in the following, the steps (1) to (6) common to the first and second embodiments will be omitted, and the steps after (7) characteristic of the present embodiment will be described.

(7) momordesine precipitation step In the momordesine precipitation step, a fine powder having a particle size of about 20 μm of bitter leaves, stems, and / or roots, as in the second momordesine extract production step in (7) of Embodiment 1, 2% of its weight is added to the extraction solution after completion of the second filtration step of (6) to precipitate momordesin crystals. Thus, by adding the fine powder of bitter melon, the fine powder can be used as a seed to efficiently precipitate momordesin.

(8) Drying step The momordesine crystals precipitated in the step are dried by spray drying under the temperature conditions of the inlet temperature of 80 degrees and the outlet temperature of 100 to 120 degrees, as in the drying step in the second embodiment (9). .

(9) Sorting process The dried powder of momordesine dried as described above is sieved to sort a substance of 100 mesh or less as in the sorting process in (10) of Embodiments 1 and 2, and this is the final processed product. And

  According to the method for recovering momordesine of Embodiment 3 described above, momordesin contained in bitter melon can be recovered with high purity, and momordesin can be recovered with fewer operations, and the labor required for recovery can be saved. .

Claims (5)

  Extracting bitter melon with ethanol to produce a bitter melon extract solution, adjusting the bitter melon extract to acidity, allowing it to stand under refrigeration, separating it into a supernatant and a precipitate, and then removing the precipitate. A method for recovering momordesine from bitter melon.   The method for recovering momordesin using bitter melon as a raw material according to claim 1, wherein fine powder of bitter melon is added to the supernatant from which the precipitate has been removed to precipitate momordesin.   A method for recovering momordesine using bitter melon as a raw material, comprising extracting bitter melon with ethanol to produce a bitter melon extract solution, and separating and recovering a substance having a molecular weight of 600 or more from the bitter melon extract solution using a molecular sieve.   4. The method for recovering momordesine using bitter melon as a raw material according to claim 3, wherein the bitter melon extract solution is fractionated into substances having molecular weights of 618, 632, and 816 by molecular sieve.   The bitter melon according to claim 3 or 4, wherein the bitter melon extract solution is adjusted to be acidic and then left to stand under refrigeration to separate the supernatant liquid and the precipitate, and then the precipitate is removed. Method for recovering momordesine using as a raw material.