JP2006234844A - Sampling device of skin permeating gas - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sampling device of a skin permeating gas constituted so as to obtain clinical data in a noninvasive/bloodless manner to contribute to home medical treatment or health control. <P>SOLUTION: The sampling device of the skin permeating gas includes a cylindrical member (31) having a spiral groove (32), the lid body (33) provided to the upper surface of the cylindrical member (31), the gas introducing part (34) connected to the lid body 33, the gas lead-out part (35) connected to the lid body (33) and the sealing member (40) of the cylindrical member (31) for hermetically sealing the air in the groove (32) of the cylindrical member (31) to set it to a predetermined temperature. After the component of the air set to the predetermined temperature is measured, the sealing member (40) of the cylindrical member (31) is detached and the cylindrical member (31), from which the sealing member (40) is detached, is set to the surface of the skin of a subject in a hermetically sealed state to sample the skin permeating gas. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a skin permeation gas collection device for measuring the physical health of a subject.

  Conventionally, blood and human metabolites (urine, stool) have been mainly used as clinical items for knowing human health, but in recent years saliva and exhaled gas are also becoming targets, and sweat Will also be included as a clinical item in the future. The components in these exhaled gas, saliva, and sweat depend on blood components, and a quantitative correlation is recognized between them. In the future, these non-invasive metabolites are expected to be used for home medical care and health management.

By the way, there has never been a measuring instrument for clinical measurement using such skin permeation gas or for checking a person's health condition. This is because the existence of the skin permeation gas itself was not assumed.
None

  As described above, the present inventors have found that the skin permeation gas depends on the blood components of peripheral blood vessels. In addition, we found that skin permeation gas is very important for knowing a new type of disease state.

  In view of the above situation, an object of the present invention is to provide a skin permeation gas collection device that can obtain clinical information by noninvasive and noninvasive blood and contribute to home medical care and health care.

In order to achieve the above object, the present invention provides
[1] In the skin permeation gas collection device, a cylindrical member having a spiral groove, a lid provided on the upper surface of the cylindrical member, a gas introduction unit connected to the lid, and the lid And measuring the component of the air set to the predetermined temperature after sealing the air in the groove of the cylindrical member and sealing the cylindrical member for setting to a predetermined temperature, The sealing member of the cylindrical member is removed, the cylindrical member from which the sealing member has been removed is set in a sealed state on the surface of the subject's skin, and skin permeation gas is collected.

  According to the present invention, the following effects can be achieved.

  (A) It is possible to obtain clinical information by noninvasive / noninvasive blood and contribute to home medical care and health management.

  (B) The skin permeation gas emanating from the skin surface can be easily collected and the type and amount of gas can be analyzed.

  (C) The collected gas can be easily sealed and can be easily transported to the measuring device.

  (D) It can be securely attached to the skin.

  (E) Contamination from the inside of the cylindrical member or the environment can be measured in advance, and skin permeation gas can be measured more accurately.

  (F) By providing the spiral flow path, the flow rate of the collected gas per unit area of the skin can be increased, and the skin permeating gas can be collected effectively.

  (G) The apparatus can be reduced in size.

  The skin permeation gas collection device of the present invention includes a cylindrical member having a spiral groove, a lid provided on the upper surface of the cylindrical member, a gas inlet connected to the lid, and the lid. And measuring the component of the air set to the predetermined temperature after sealing the air in the groove of the cylindrical member and sealing the cylindrical member for setting to a predetermined temperature, The sealing member of the cylindrical member is removed, the cylindrical member from which the sealing member has been removed is set in a sealed state on the surface of the subject's skin, and the skin permeating gas is collected.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

  FIG. 1 is a configuration diagram of a skin permeation gas collecting apparatus showing a first reference example of the present invention, FIG. 1 (a) is an external perspective view, and FIG. 1 (b) is a skin permeation gas collecting apparatus. FIG. 1 (c) is a diagram showing a state where the skin permeation gas is circulated from the lower tank to the upper tank, and FIG. 1 (d) is a diagram showing the state where the upper tank is filled. It is a figure which shows the state which sealed the performed skin permeation | transmission gas.

  In these drawings, 1 is a container of a skin permeation gas collecting apparatus, 1A is a lower tank in the container 1, 1B is an upper tank in the container 1, and 2 is arranged in the lower part in the container 1, A partition plate having an opening 2A, 3 is an operation member capable of opening and closing the opening 2A of the partition plate 2, 4 is a knob provided at the upper end of the operation member 3, 5 is a rod of the operation member 3, and 6 is the rod. 5 is a valve member provided at the lower end of 5, 7 is an O-ring for sealing between the container 1 and the rod 5, 8 is an air outlet for exhausting skin permeation gas provided in the container 1, and 9 is skin provided in the container 1 The permeate gas outlet 10 is the skin of the subject.

  The operation of this apparatus will be described below.

  First, as shown in FIG. 1 (b), the operation member 3 is pushed down, and the container 1 of the skin permeation gas collecting device is placed on the skin 10 of the subject with the opening 2 </ b> A of the partition plate 2 closed by the valve member 6. Press and fix. In this state, the skin permeation gas from the skin 10 of the subject is collected in the lower tank 1A. Here, the air outlet for exhausting the skin permeation gas provided in the container 1 and the skin permeation gas outlet 9 are closed.

  Next, when the collection of the skin permeating gas is finished, the operating member 3 is pulled up to open the opening 2A of the partition plate 2 as shown in FIG. Then, the skin permeation gas collected in the lower tank 1A flows to the upper tank 1B.

  Next, as shown in FIG. 1 (d), the operation member 3 is pushed down again to close the opening 2A of the partition plate 2, and the skin permeating gas is confined and stored in the upper tank 1B.

  Next, although not shown, the skin permeation gas outlet 9 is opened, the air blowing port 8 is also opened, the skin permeating gas is expelled by ventilation from the air blowing port 8, and the skin permeating gas is used for measurement.

  FIG. 2 is a block diagram of a skin permeation gas collecting apparatus showing a second reference example of the present invention.

  In this figure, 11 is a first container for storing skin-permeating gas, 12 is a second container that surrounds the container 11 and depressurizes by removing air to bring it into close contact with the skin, and 13 is a second container 12. Exhaust device for exhaust connected, 14 is a cock, 15 is a gas introducing device connected to the first container 11, 16 is a derivation device for skin permeation gas connected to the first container 11, and 17 is a subject. The skin.

  In this way, after the derivation device 16 is closed and the gas introduction device 15 is opened and gas (dry air) is introduced into the first container 11, the gas introduction device 15 is closed and the skin 17 of the subject permeates the skin. Collect gas. When the skin permeation gas is collected, the outlet device 16 is opened and the skin permeation gas is collected in a test container (not shown).

  In that case, the container portion has a double structure in order to make the first container 11 adhere to the skin 16 of the subject. Specifically, the inside of the second container 12 disposed outside the first container 11 can be depressurized using the exhaust device 13 to achieve close contact with the skin.

  FIG. 3 is a configuration diagram of a skin permeation gas collecting apparatus showing a third reference example of the present invention, FIG. 3 (a) is a diagram showing a finger sac and a syringe connected thereto, and FIG. 3 (b) is a diagram showing a finger sack. FIG. 3 (c) is a view showing a state where the finger sack is attached, FIG. 3 (c) is a view showing a state where the finger sack is filled with air, and FIG. 3 (d) is a view showing a state where the air and skin permeation gas are inhaled. is there.

  In this figure, 21 is a finger sack (a Teflon (registered trademark) owl), 22 is a connector (for example, a cock), 23 is a syringe (100 ml), 24 is a stopcock, and 25 is the outside of the finger sack 22 at the base. A rubber or clip to be attached to, 26 is a finger of a subject, 27 is a gas introducing device, and 28 is a finger sack in a state of being inflated with air.

  According to this embodiment, the skin permeation gas can be recovered by the following three methods.

[1] First Method As shown in FIG. 3A, a finger sack 21 having a cock 22 at the tip and a syringe 23 having a stop cock 24 connectable to the cock 22 are prepared. As shown in b), the finger sack 21 is attached to the finger 26. Next, as shown in FIG. 3 (c), the stop cock 24 is opened, the cock 22 is opened, gas (dry air) is introduced from the syringe 23, and the finger sack 21 is inflated and then sealed. Then, stop cock 24 is closed. Therefore, skin permeation gas is collected from the subject's finger 26. When the skin permeation gas can be collected, the skin permeation gas is collected in the syringe 23 through the cock 22 by opening the stop cock 24.

[2] Second Method As shown in FIG. 3A, a finger sack 21 having a cock 22 at the tip and a syringe 23 having a stop cock 24 connectable to the cock 22 are prepared. As shown in b), the finger sack 21 is attached to the finger 26. Next, as shown in FIG. 3 (c), the stopcock 24 is closed, the gas introduction device 27 is opened to introduce gas (dry air), the finger sac 21 is inflated and then sealed, and the skin permeating gas is obtained. To collect. When the skin permeation gas can be collected, the skin permeation gas is collected into the syringe 23 through the cock 22 with the stop cock 24 opened.

[3] Third Method As shown in FIG. 3A, a finger sack 21 having a cock 22 at the tip and a syringe 23 having a stop cock 24 connectable to the cock 22 are prepared. As shown in b), the finger sack 21 is attached to the finger 26. Next, as shown in FIG. 3C, the stopcock 24 is opened, the gas introduction device 27 is opened, and the gas (dry air) is introduced, and the skin permeation gas is collected into the syringe 23.

  Without limiting to the above, the gas is introduced from the cock 22 to collect the skin permeation gas, and the skin permeation gas is collected into the syringe 23, or the gas is introduced from the gas introduction device 27, and the skin. Various modifications such as collecting the permeate gas and collecting it from the cock 22 are possible.

  By comprising in this way, the emitted gas can be easily collected and collect | recovered from the body skin surface, the skin surface of a finger circumference only here.

  Hereinafter, an experimental example using this sampling method will be described.

  FIG. 4 is a diagram comparing a breath and acetone generated from the left index finger using the apparatus of the present invention.

  When collecting acetone generated from the left index finger, exhalation was also collected at the same time as the acetone sampling start time, and the results of comparing the respective acetone concentrations are shown in FIG. Referring to this figure, there is a positive correlation between exhaled breath and acetone emitted from the left index finger.

  As described above, outside air was used this time in the collection and quantification of acetone generated from the left index finger. This is because the surrounding air always enters the finger sack when the finger is put into the finger sack at the time of sampling.

  In using this method, it is assumed that the amount of acetone in the outside air does not change in a short time (within 30 minutes). Actually, it does not always show a constant value, so if the change in the amount of acetone exceeds the error range, the determination of acetone will be suspicious by this method. Therefore, in order to improve this problem, it is necessary to adopt a finger sack and a sample collecting method in which ambient air does not enter when the finger is taken in and out.

  FIG. 5 is a block diagram of a skin permeation gas collecting apparatus (sampling probe) showing an embodiment of the present invention.

  In this figure, 31 is a cylindrical member having a spiral groove 32, 33 is a lid provided on the upper surface of the cylindrical member 31, 34 is a gas introduction part connected to the lid 33, and 35 is connected to the lid 33. 40 is a cylindrical member sealing member that seals the air in the groove 32 of the cylindrical member 31 and sets it to a predetermined temperature, 41 is a heat sink, and 42 is a Peltier element that sets the temperature of the sealed air. , 43 is a silicon sheet, and 44 is a Teflon (registered trademark) sheet, which seals the air in the groove 32.

  Here, first, air in the laboratory is introduced from the gas introduction unit 34 and sealed in the cylindrical member 31. After the air introduced by the Peltier element 42 is brought to a predetermined temperature, the air is led out from the gas lead-out unit 35. Measure the air (probe blank). Next, in place of the cylindrical member sealing member 40, the bottom surface of the cylindrical member 31 is brought into contact with the skin 45 of the subject and sealed, and the skin permeating gas is collected in the groove 32 of the cylindrical member 31. The collected skin permeation gas is led out together with air from the gas lead-out unit 35 and measured.

  The measurement results are shown below.

  Table 1 shows the measurement results.

この表から明らかなように、被験者ａの場合、測定器からの空気量は１．８ｎｇ、被験者の皮膚透過ガスであるアセトンを加えた量が４．４ｎｇであり、被験者の皮膚透過表面から放出されたアセトンガスは２．６ｎｇであることが分かる。皮膚のサンプリングはプローブブランクよりいずれの被験者においても高値であった。

As is clear from this table, in the case of the subject a, the amount of air from the measuring device is 1.8 ng, and the amount of acetone, which is the subject's skin permeating gas, is 4.4 ng. It can be seen that the acetone gas applied is 2.6 ng. Skin sampling was higher in all subjects than the probe blank.

  The amounts of acetone in the indoor air of 50 ml, 25 ml, and 10 ml were 5.8, 2.8, and 1.3 ng, respectively. Since the sampling probe volume was 3.8 ml, the contamination from the environment was 0.5 ng or less. The acetone concentration in the laboratory air was almost equal to the amount of acetone in the indoor air of the reinforced concrete building.

  From the above, it can be said that the amount of acetone released from the surface of human skin can be measured with the experimental means.

FIG. 6 is a graph showing the relationship between BMI {body mass index, body weight (kg) / [height (m)] ² )}, which is an index of obesity, and the amount of acetone released using the apparatus of the present invention.

  As is clear from this figure, a tendency of a positive correlation was observed between the degree of obesity (BMI) and the amount of acetone released.

  The collected gas from the human skin surface is quantified, for example, by chromatography.

  FIG. 7 is a diagram showing a chromatographic analysis result when the probe blank of the present invention is sampled for 1 minute inside the left forearm. Here, the upper part of FIG. 7 is a diagram showing the analysis result of the chromatograph when the skin permeation gas collecting apparatus of the present invention is used and sampling is performed for 10 minutes inside the left forearm. The lower part of FIG. 7 is a diagram showing the analysis result of the probe blank chromatograph. As is apparent from this figure, according to the skin permeation gas collecting device, the release of the skin permeation gas on the human skin surface is larger than that present in the environment or as a probe blank.

  8 to 11 are diagrams showing the relationship between the intake of milk and the release of hydrogen gas using the skin permeation gas collecting apparatus of the present invention. The breath and left hand of subject A (before drinking milk, drinking about 4 (After time), the hydrogen chromatogram measured for the breath and left hand of subject B (before drinking milk, about 4 hours after drinking) is shown.

  FIG. 8 shows a chromatogram of hydrogen in exhaled breath. Chromatograms a and b are before and after subject A drinks milk. 8A and 8C, the sensitivity is 5 mV, and FIGS. 8B and 8D are the sensitivity of 100 mV.

  FIG. 9 shows the result of collecting and analyzing skin permeation gas from the left hand for 10 minutes. About 4 hours after the subject drinks milk, the sensitivity is 2 mV in FIG.

  As is clear from these figures, hydrogen is released from the human skin surface, and this hydrogen can be detected by the apparatus of the present invention. In particular, it was found that the intake of milk and its correlation could clarify it.

  When comparing the hydrogen concentration before drinking milk and about 4 hours after drinking milk, the breath increased from 2 to 7 ppm in subject A, from 1 to 91 ppm in subject B, and from hydrogen from the left hand, subject It increased from 0.003 to 0.08 ppm in A and from 0.07 to 0.41 ppm in subject B, and it was found that the generation was promoted by drinking milk not only in exhaled breath but also in hydrogen generated from the left hand. It was. Furthermore, from this measurement example, a very good correlation is obtained between the hydrogen concentration in the breath and the hydrogen concentration released from the skin.

  10 and 11 show the correlation between exhaled breath and skin permeating gas. A clear correlation was found for hydrogen gas, indicating that diagnosis by skin permeation gas is possible.

  FIG. 12 is a block diagram of a skin permeation gas measuring apparatus showing a fourth reference example of the present invention.

  In this figure, 50 is a skin permeation gas measuring device, 51 is a skin permeation gas collection tank, 52 is a circulation tank, 53 is a blower fan, 54 is various coloring reagents A, B, C, and 55 are the skin of the subject.

  As shown in this figure, the specific gas can be detected by collecting the skin permeation gas on the skin 55 and circulating the skin permeation gas with the blower fan 53 and passing the various color developing reagents 54 through. . Thereby, a test subject can know a result on the spot.

  Although not shown, the information processing of the sampling / detecting system installed on the skin may be performed by an incorporated operator.

In addition, in order to avoid absorption of gas into H ₂ O of perspiration, sufficient gas replacement is performed. In other words, it is important that the gas is collected while being purged by the gas flow after sampling and the gas being sampled is continuously purged.

  Hereinafter, the use of the present invention will be described.

  Regarding skin permeation gas and pathology, if the skin permeation gas is acetone gas, it is related to diabetes, obesity, and self-poisoning. If the skin permeation gas is hydrogen, the presence and amount of intestinal bacteria to break down lactose There is a relationship. When the skin permeation gas is Ammonia, it is associated with uremia, liver failure, abdominal abscess, and H. pylori. Thus, the measurement of skin permeation gas according to the present invention can contribute to the diagnosis of various pathological conditions.

  In addition, this invention is not limited to the said Example, A various deformation | transformation is possible based on the meaning of this invention, and these are not excluded from the scope of the present invention.

  The skin permeation gas collection device of the present invention can be used as a skin permeation gas collection device that obtains clinical information by noninvasive and noninvasive observation and contributes to home medical care and health management.

It is a block diagram of the skin permeation gas collection device which shows the 1st reference example of the present invention. It is a block diagram of the skin permeation gas collection apparatus which shows the 2nd reference example of this invention. It is a block diagram of the skin permeation gas collection apparatus which shows the 3rd reference example of this invention. It is the figure which compared the acetone which generate | occur | produces from expiration and the left index finger using the apparatus of this invention. It is a block diagram of the skin permeation gas collection device (sampling probe) which shows the Example of this invention. It is a figure which shows the relationship between BMI which is a parameter | index of obesity, and acetone discharge | release amount using the apparatus of this invention. It is a figure which shows the chromatogram at the time of sampling the probe blank of this invention for 10 minutes inside the left forearm. It is a figure (the 1) which shows the relationship between intake of milk and discharge | release of hydrogen gas using the skin permeation gas collection apparatus of this invention. It is a figure (the 2) which shows the relationship between intake of milk and discharge | release of hydrogen gas using the skin permeation gas collection apparatus of this invention. It is a figure (the 3) which shows the relationship between intake of milk and discharge | release of hydrogen gas using the skin permeation gas collection apparatus of this invention. It is FIG. (The 4) which shows the relationship between intake of milk and discharge | release of hydrogen gas using the skin permeation gas collection apparatus of this invention. It is a block diagram of the skin permeation gas measuring apparatus which shows the 4th reference example of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Container of skin permeation gas collection apparatus 1A Lower tank in container 1B Upper tank in container 2 Partition plate 2A Opening 3 Operation member 4 Knob 5 Rod 6 Valve member 7 O-ring 8 Skin permeation gas outlet 9, 9, 45, 55 Subject skin 11 First container for storing skin permeation gas 12 Second container for bringing skin into close contact 13 Exhaust device 14 Cock 15 Gas introduction device 16 Skin permeation gas derivation device 17, 26 Subject's finger 21 finger sack 22 connector (cock)
Reference Signs List 23 Syringe 24 Stop cock 27 Gas introduction device 28 Finger sack 31 filled with air and inflated 31 Cylindrical member 32 Spiral groove 33 Lid 34 Gas introduction part 35 Gas outlet part 40 Cylindrical member sealing member 41 Heat sink 42 Peltier element 43 Silicon sheet 44 Teflon (registered trademark) sheet 50 Skin permeation gas measuring device 51 Skin permeation gas collection tank 52 Circulation tank 53 Blower fan 54 Various coloring reagents A, B, C

Claims (1)

(A) a cylindrical member having a spiral groove;
(B) a lid provided on the upper surface of the cylindrical member;
(C) a gas inlet connected to the lid;
(D) a gas outlet portion connected to the lid;
(E) sealing the air in the groove of the cylindrical member and setting the cylindrical member at a predetermined temperature; and
(F) After measuring the component of air set to the predetermined temperature, the cylindrical member is removed, and the cylindrical member from which the sealing member has been removed is set in a sealed state on the surface of the subject's skin. And collecting the skin permeation gas.

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