JP2006231124A - Selective separation and recovery apparatus and method of bacterial by foam separation method using protein - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a selective separation and recovery apparatus and method of bacterial by foam separation method using protein. <P>SOLUTION: A plurality of bacteria-containing water to be treated is poured into a treatment tank, and protein selectively adsorbing the bacteria is fed into the water to be treated and at the same time bubbles are generated in the water by an air supply means. A least one among one colon bacillus, Escherichia coli, one Enterococcus, Enterococcus facalis, two streptococci, Streptococcus dysgalactia and Streptococcus iniae, and one Pasteurella bacterium, Pasteurella piscucuda is selectively adsorbed by the protein in the bubbles to be separated and recovered. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for selectively separating and recovering bacteria from treated water containing a plurality of bacteria, and more particularly to a method for selectively separating and recovering bacteria by a foam separation method using proteins. The present invention relates to a separation and recovery device to be used.

  Currently, the water in river water, pools, public baths, and beaches, which are raw water for tap water, contains a relatively low concentration of bacteria, and the tolerance for human use is determined by the concentration. ing. However, in recent years, there have been accidents in which pathogenic bacteria are mixed in these bacteria, infecting animals and fishery products, and indirectly or directly infecting the human body.

  In order to prevent the above-mentioned accident, sterilization treatment with various chemicals and the like is performed. For example, chlorine and ozone are harmful to the human body and there are restrictions on the usage. In addition, as a method for separating these bacteria, a plate method using an agar plate is widely adopted. However, it takes at least several days to cultivate the bacteria, and since only a small amount of sample can be inoculated, a treatment with low bacterial density is performed. In the case of water, there was a problem that bacteria could not be detected. In addition, there is a culture method using suction filtration and a filter, but it is necessary to filter a large amount of treated water, which requires a long time and labor for the work, and there is a possibility that bacterial cells may be destroyed during the suction.

In order to solve these problems, a solid-liquid separation failure inhibitor and a solid-liquid separation failure suppression method for suppressing solid-liquid separation failure due to filamentous bulking in an activated sludge treatment system have been proposed (Patent Document 1). reference.). In addition, a technique for separating a target cell by treating a cell solution to which a modified antibody is bound with a water-insoluble carrier containing a carboxyl group on its surface has also been introduced (see Patent Document 2).
JP-A-11-216484 Japanese Patent Application Laid-Open No. 7-120452

  However, in the case of the solid-liquid separation failure suppression method proposed in Patent Document 1, a surfactant and a granular carrier are required, and the method is limited to the treatment of filamentous bacteria. There is a problem that bacteria cannot be selectively separated and collected from river water, pools, public baths, and beaches that are raw water of tap water mixed at low concentrations. In addition, the technique introduced in Patent Document 2 is useful for cell separation in biochemical research and specific cell collection in the medical field, but is not suitable as a method for separating bacteria mixed in the treated water. It is.

  In view of the above problems, the present inventors have paid attention to the fact that bacteria tend to adhere to proteins such as biological mucous membranes and adherents on the body surface. It is an object of the present invention to provide an apparatus and a collection method for selectively separating and recovering bacteria from treated water containing a plurality of bacteria.

  Therefore, in the separation and recovery method of the present invention, treated water containing a plurality of bacteria is injected into a treatment tank, a protein that selectively adsorbs the bacteria is introduced into the treated water, and the inside of the treated water is supplied by an air supply means. Bubbles are generated, and the bacteria are selectively adsorbed to the protein in the bubbles to be separated and recovered.

  In addition, the bacteria to be separated and recovered are at least Escherichia coli Escherichia coli, Enterococcus Enterococcus facalis, Streptococcus 2 Streptococcus dysgalactia, Streptococcus iniae, Pasteurella sp. .

  The separation and recovery apparatus according to the present invention includes a means for injecting treated water containing a plurality of bacteria into a treatment tank, a means for introducing a protein that selectively adsorbs bacteria into the treated water, and an air supply means. And a means for selectively adsorbing the bacteria to the protein in the bubbles to separate and recover the bacteria.

  In the present invention, examples of proteins used include fish body surface mucilage (glycoprotein), casein, albumin, hemoglobin, soy protein, gelatin and the like. .

  According to the present invention, since bacteria can be separated and recovered from treated water without using harmful substances such as chlorine and ozone, it has an excellent effect that the operation is extremely safe.

  In addition, protein is introduced into the treated water, bubbles are generated in the treated water, and bacteria are selectively adsorbed to the protein for separation and recovery, so the work time is short and even treated water with low bacterial density is efficient. It has an excellent effect that bacteria can be separated and recovered well.

  Furthermore, since the separation / recovery device of the present invention adsorbs bacteria by bubbles and proteins generated by the air supply means without using suction filtration or filtering, it has an excellent effect that the bacteria can be separated and recovered without being destroyed.

  EXAMPLES Hereinafter, although this invention is demonstrated based on an Example, it cannot be overemphasized that this invention is not limited to a present Example. Moreover, the suspending water in a present Example mentioned later shows the said treated water.

  FIG. 1 shows an embodiment of a separation / recovery device according to the present invention. The separation / recovery device 1 comprises a substantially cylindrical processing tank 2, an air feed pump 3, a glass ball filter 4, and an aspirator 5. An air flow meter 6 is connected between the air feed pump 3 and the processing tank 2, and the foam trap bottle 7 is connected between the aspirator 5 and the processing tank 2.

  The raw water injected into the treatment tank 2 is supplied with protein (not shown) from a protein input device (not shown) to form suspended water 8, and the air supplied from the air supply pump 3 is the glass ball filter 4. And the suspended water 8 in the treatment tank 2 is stirred and raised. Foam 9 is generated by the protein contained in the suspension water 8 and the fine bubbles, and bacteria are selectively adsorbed by the protein in the foam 9. The foam 9 collected on the liquid surface of the processing tank 2 is sucked by the aspirator 5 and collected in the foam trap bottle 7. Hereinafter, the method for selectively separating and recovering bacteria by the foam separation method using the protein according to the present invention will be described in more detail.

(Cultivation of test bacteria and injection into raw water)
E. coli Escherichia coli, Enterococcus enterococcus Enterococcus facalis, Streptococcus species 2 Streptococcus dysgalactia, Streptococcus iniae, Pasteurella piscucuda at 25 ° C. are cultured for 24 hours at 25 ° C. Next, the cultured bacteria were treated with a centrifuge at 6000 rpm for 30 minutes to collect the bacteria, and poured into 250 ml of sterilized water (raw water). Further, sterilized water (raw water) was added to a predetermined turbidity and diluted to obtain suspended water.

(Test protein)
The live fish was frozen at −30 ° C. by a freezer, thawed again, immersed in 1 l of ethanol and left for 5 minutes. Next, the gelled sticky material is peeled off from the surface of the live fish with a spoonful, filtered with suction through a GF / C filter, further washed with ethanol several times, dried under reduced pressure, and powdered with a pulverizer. A viscous powder was obtained. Casein, albumin, hemoglobin, soy protein, and gelatin were commercially available. Gelatin was dissolved in hot distilled water at 50 to 60 ° C., and the others were dissolved in 0.01 mol aqueous sodium hydroxide solution to obtain an aqueous protein solution. In addition, the live fish in a present Example used the eel and the carp.

(Foam separation)
200 ml of suspended water was put into a 300 ml beaker, the prepared protein aqueous solution was added to a predetermined concentration, and rapidly stirred for 1 minute with a jar tester. The addition concentration was such that foam was generated on the surface of the turbid water. Next, 200 ml of this turbid water was collected and poured into the treatment tank of the separation and recovery device to perform foam separation. The foam separation treatment conditions were an air flow rate of 0.3 l / min and a treatment time of 3 minutes. The separation rate was determined from the absorbance of the turbidity of the raw suspended water and the treated water after separation. The results are shown in Table 1.

  As shown in Table 1, enterococci, streptococci streptococcus dysgalactia, and pasteurella were selectively separated and recovered by the above proteins.

  According to the present invention, it can be used as a highly sensitive monitoring technique for pathogenic bacteria in extremely low-density bacterial test water such as tap water raw water. In addition, since bacteria can be separated without using sterilizing agents such as chlorine and ozone, which are dangerous substances, it can be used as a sterilization technique for water in pools, public baths, fishing port fish markets and the like.

It is explanatory drawing which shows one Example of the separation-and-recovery apparatus which concerns on this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Separation and recovery equipment 2 Processing tank 3 Glass ball filter 4 Air supply pump 5 Aspirator 6 Air flow meter 7 Foam trap bottle 8 Suspended water 9 Foam

Claims (3)

  A treatment water containing a plurality of bacteria is injected into a treatment tank, a protein that selectively adsorbs the bacteria is introduced into the treatment water, and air bubbles are generated in the treatment water by an air supply means, whereby the bacteria are A method for selectively separating and recovering bacteria by a foam separation method using a protein, wherein the protein is selectively adsorbed on the protein in bubbles and separated and recovered.   The bacterium to be separated and recovered is Escherichia coli 1 type, Enterococcus 1 type Enterococcus facalis, Streptococcus 2 types Streptococcus dysgalactia, Streptococcus iniae, Pasteurella sp. 1 type. A method for selective separation and recovery of bacteria by a foam separation method using the protein according to 1. Means for injecting treated water containing a plurality of bacteria into a treatment tank; means for introducing a protein that selectively adsorbs the bacteria into the treated water; means for generating bubbles in the treated water by an air supply means; The apparatus for selectively separating and recovering bacteria by a foam separation method using a protein according to claim 1 or 2, comprising means for selectively adsorbing the protein to the protein in the bubbles and separating and recovering the same.

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