JP2006223168A - Examination tool - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an examination tool by which a medium for examination can be readily prepared in a short time. <P>SOLUTION: The examination tool is obtained by accomodating, in the inside of a tube-shaped container 12 of a packaged body 10, a first content 20, and an inner packaged body 16 encapsulating a second content from which the medium for the examination is prepared by being mixed with the first content, and ruptured by a deforming force added to a packaging body 10 from the exterior, and sealing the packaged body 10. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to the preparation of a medium used for separating and culturing microorganisms in a microorganism test for separating and searching for microorganisms such as bacteria, and cooking utensils, cooking tables, and dishes in machinery and restaurants in food factories. It is related with the test | inspection tool used, for example, when inspecting whether nutrient components, such as protein and starch which become a factor of microbial contamination remain, after washing | cleaning.

For example, when culturing anaerobic bacteria, place the medium inoculated with anaerobic bacteria and an oxygen scavenger in a sealable container or bag, and use a pipette or the like to add a certain amount of purified water to the oxygen scavenger. When the reaction between the oxygen scavenger and purified water is started, the container (bag) is sealed, and anaerobic bacteria are cultured in the sealed container (sealed bag) (see, for example, Patent Document 1). In addition, when selectively cultivating Salmonella bacteria, a certain amount of iodine solution (aqueous solution of iodine and potassium iodide) is added to the Hana tetrathionate basal medium, and thiosulfate, a component in the basal medium. Potassium tetrathionate is produced by the reaction of the salt with iodine and potassium iodide, and Salmonella bacteria are grown on a Hanna tetrathionate medium containing this potassium tetrathionate.
(For example, refer to Patent Document 2).

  In addition, when a bacterial test is performed, when a target bacterium is present in a small amount in a specimen or the target bacterium is damaged, a selective medium is used from the beginning. In some cases, the cells themselves are damaged, and the target bacteria cannot be propagated, resulting in a negative culture result. Therefore, first, by culturing using a non-selective medium, the target bacteria and miscellaneous bacteria are allowed to grow, and then a part thereof is inoculated into the selective medium to grow the target bacteria. As another method, first, inoculate a sample on a non-selective medium, and after a small amount of target bacteria have been grown or recovered from damaged bacteria, a selection agent is added to the medium after, for example, about 6 hours, For example, suppression of the growth of bacteria other than the target bacteria is performed.

In addition, when examining changes in pH or redox potential of the medium during bacterial culture, a dye such as phenol red or methyl red is added to the medium in advance. Then, by observing changes in the color of the medium, changes in the pH and redox potential of the medium are detected.
Japanese Examined Patent Publication No. 4-61635 (page 2-3) JP 2002-243743 A (pages 7-8)

  As described above, when culturing a microorganism, a certain amount of purified water is dispensed into a medium and an oxygen scavenger using a pipette, or a certain amount of iodine solution is added to a basal medium. In the method of preparing the test medium, the preparation operation takes time and effort. In addition, after culturing target bacteria and miscellaneous bacteria using a non-selective medium, a method of inoculating a part of the culture into a selective medium or adding a selective agent to the medium is carried out by transplanting bacteria during the cultivation. The operation of adding a selective agent is troublesome and takes time. In addition, pigments are known to suppress the growth of bacteria, but the method of adding pigments to the medium in advance slows down the growth rate of bacteria and inhibits the growth of bacteria, so it takes time to test. In addition, there is a problem that a reliable inspection cannot be performed.

  The present invention has been made in view of the circumstances as described above, and is an inspection tool that can easily prepare a test medium in a short time. It is an object of the present invention to provide an inspection tool that does not require an operation for addition, is simple and does not take time, and an inspection tool that can quickly and surely perform an inspection related to microorganisms.

  The invention according to claim 1 is a testing tool for preparing a culture medium for microbiological testing, wherein at least a part of the first content in a liquid or powder form is accommodated in a package having flexibility or flexibility. In addition, a liquid, powdery or gaseous second content for preparing a test medium by mixing with the first content is enclosed and ruptured by a deformation force applied to the package from the outside. One or two or more internal packaging bodies are accommodated, and the packaging body is sealed.

  The invention according to claim 2 is the inspection tool according to claim 1, wherein the first content is a medium and an oxygen scavenger, the second content is purified water, and the test is for anaerobic culture. A preparation medium is prepared.

  The invention according to claim 3 is the inspection tool according to claim 1, wherein the first content is a Hana-tetrathionate basal medium, the second content is an iodine solution, and the Salmonella bacterium A test medium that is a selective enrichment medium is prepared.

  According to a fourth aspect of the present invention, there is provided a test medium according to the first aspect, wherein the first content is sterilized purified water, the second content is a powder culture medium, and the liquid culture medium is a test medium. It is characterized by preparing.

  The invention according to claim 5 is the inspection tool according to claim 1, wherein the second content is a powdered part of the components of the liquid medium, and the first content is the liquid medium. A test medium, which is a solution containing other components and is a liquid medium, is prepared.

  The invention according to claim 6 is the inspection tool according to claim 1, wherein the second content is a concentrated medium, the first content is a diluent, and the test medium is a liquid medium. It is characterized by doing.

  The invention according to claim 7 is the inspection tool according to claim 6, wherein the package is formed of a flexible resin material and is provided with a tube-shaped container provided with an opening, and the opening of the tube-shaped container. It is composed of a cap that is detachably fitted, and a swab for wiping inspection is attached to the inside of the cap, and a cotton ball of the swab is immersed in the diluent.

  The invention according to claim 8 is the inspection tool according to claim 7, further comprising an inner package that encloses the disinfectant and ruptures by a deformation force applied to the package from outside. It is characterized by that.

  The invention according to claim 9 is an inspection tool for storing a culture medium for microbiological testing, wherein the culture medium is stored in a package having at least a part of flexibility or flexibility, and an additive is enclosed therein. One or two or more internal packaging bodies that are ruptured by a deformation force applied from the outside to the packaging body are accommodated, and the packaging body is sealed.

  The invention according to claim 10 is the inspection tool according to claim 9, wherein the medium is a non-selective medium for growing target bacteria and various germs, and the additive suppresses the growth of germs other than the target bacteria. It is a selective agent.

  The invention according to claim 11 is the inspection tool according to claim 9, wherein the additive is an inspection dye.

  The invention according to claim 12 is an inspection tool for inspecting the presence or absence of a contamination factor that causes microbial contamination, and a container having at least a part of flexibility or flexibility and provided with an opening, and this Microorganisms are formed in a cap that is detachably fitted into the opening of the container, and a swab for wiping inspection is attached to the inside of the cap and the swab is inserted into the container. One or two or more internal packaging bodies that contain a reagent that reacts with a pollutant substance that causes contamination and are ruptured by a deformation force applied to the packaging body from the outside are contained, and the packaging body is sealed. It is characterized by.

  The invention according to claim 13 is the inspection tool according to any one of claims 1 to 12, wherein the inner package is formed of a film material in a sealed bag shape and at least a part of the peripheral edge is weakly bonded. It is characterized by that.

In the inspection tool of the invention according to claim 1, by applying a deforming force to the package by pressing the outer surface of the package with a finger, the internal package accommodated in the package is ruptured, and the internal package The second content flows out of the container, and the first content and the second content accommodated in the package are mixed to prepare a test medium.
Therefore, when the inspection tool of the invention according to claim 1 is used, the inspection medium can be prepared easily and in a short time.

  In the inspection tool of the invention according to claim 2, a test medium for anaerobic culture can be prepared by reacting purified water that has flowed out of the inner package with an oxygen scavenger contained in the package. .

  In the inspection tool of the invention according to claim 3, by reacting iodine and potassium iodide of the iodine solution flowing out from the inner package and the thiosulfate of the Hana tetrathionate basal medium contained in the package, A selective enrichment medium for Salmonella bacteria can be prepared.

  In the inspection tool of the invention according to claim 4, the liquid medium can be prepared by dissolving the powder medium flowing out from the inner package in sterilized purified water contained in the package.

  In the inspection tool of the invention according to claim 5, the required components are mixed by mixing a part of the components of the liquid medium flowing out from the inner package with a solution containing other components of the liquid medium contained in the package. A liquid medium containing it can be prepared.

  In the inspection tool of the invention according to claim 6, a liquid medium having a predetermined concentration can be prepared by diluting the concentrated medium flowing out from the inner package with a diluent contained in the package.

In the inspection tool of the invention according to claim 7, when the swab attached to the inner side of the cap is stored in a state of being immersed in a diluent contained in the tube-like container and performing a wiping inspection, the tube Remove the cap from the opening of the container. At this time, the cotton ball of the cotton swab attached to the cap is wet with the diluent, and the sample is collected by wiping the surface of the site to be inspected with this wet cotton ball. After collecting the sample, a cap is fitted into the opening of the tube-shaped container to seal the tube-shaped container, and the inner package is ruptured to complete a liquid medium of a predetermined concentration, and the microorganism is cultured in this liquid medium. Search for microorganisms.
Therefore, when the inspection tool of the invention according to claim 7 is used, it is possible to efficiently perform a series of operations from sampling to microbial culture in the wiping inspection in a short time, and to save the labor for preparing the medium. .

In the inspection tool of the invention according to claim 8, after the microorganism is cultured in the liquid medium and the inspection is completed, the outer surface of the tubular container is pushed with a finger to apply a deformation force to the tubular container, Another medium package accommodated can be ruptured, the disinfectant can flow out of the internal package, and the medium can be disinfected by mixing the disinfectant with the medium in which the microorganisms have propagated.
Therefore, when the inspection tool of the invention according to claim 8 is used, the culture medium can be safely discarded after the wiping inspection.

In the inspection tool of the invention according to claim 9, by applying a deformation force to the package by pressing the outer surface of the package with a finger, the internal package accommodated in the package is ruptured, and the internal package From which the additive flows out, and the additive is added to the medium contained in the package.
Therefore, when the inspection tool of the invention according to claim 9 is used, the operation of adding the additive to the culture medium can be performed easily and in a short time.

  In the inspection tool of the invention according to claim 10, after the target bacteria and various germs are grown to some extent in the non-selective medium contained in the package, the selective agent that has flowed out of the internal package is added to the non-selective medium. By this, it is possible to suppress the growth of various bacteria other than the target bacteria.

In the inspection tool of the invention according to claim 11, after the microorganisms are grown as usual in the medium contained in the package, the color of the medium is changed by adding the test dye flowing out from the inner package to the medium. By observing the change, for example, a change in the pH or redox potential of the medium can be sensed.
Therefore, when the inspection tool of the invention according to claim 11 is used, microbial inspection can be performed promptly and reliably.

In the inspection tool of the invention according to claim 12, when performing a wiping inspection, the cap is removed from the opening of the container, and the surface of the inspected part is wiped off with a cotton swab attached to the cap, and a sample is collected. To do. After the sample is collected, the cap is fitted into the opening of the container, and then the outer packaging of the container is ruptured by pushing the outer surface of the container with a finger and applying a deformation force to the container. The reagent flows out of the package, and the reagent reacts with the pollutant substance adhering to the cotton ball of the cotton swab, whereby the presence or absence of the pollutant substance can be inspected. And, before using the inspection tool, the cotton swab is inserted into the sealed package container, and the reagent contained in the container together with the cotton swab is in a state of being enclosed in the inner package, The swab is kept clean.
Therefore, when the inspection tool according to the twelfth aspect of the present invention is used, it is possible to promptly and surely inspect the pollutant substances that cause microbial contamination.

  In the inspection tool according to the thirteenth aspect of the present invention, when a deformation force is applied to the package body, the peripheral edge portion of the inner package body that is weakly bonded easily peels and bursts.

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the best embodiment of the present invention will be described with reference to the drawings.
FIG. 1 is a perspective view showing an example of an embodiment of the present invention, with a part of an inspection tool broken.

  This inspection tool is configured by accommodating an internal package 16 inside a package 10 composed of a tubular container 12 and a cap 14. The tubular container 12 is formed of a flexible resin material that is easily deformed by picking with a fingertip, and has an opening at the top. A cap 14 is detachably fitted into the opening of the tubular container 12 so that the package 10 can be sealed. The internal packaging body 16 is formed so as to be ruptured by picking and deforming the packaging body 10 with a fingertip and applying a force from the outside of the packaging body 10. For example, the inner package 16 is formed of a film material in a sealed bag shape, and has a configuration in which a part of the peripheral edge portion 18 is weakly bonded by thermal bonding or the like. When the deforming force is applied to the package 10, the weakly bonded peripheral edge 18 is easily peeled off, and the inner package 16 is ruptured.

  A liquid or powdery first content 20 is accommodated in the tubular container 12 of the package 10. In addition, in the inner package 16, a liquid, powdery or gaseous second content for preparing a test medium by mixing with the first content 20 is enclosed. Specifically, for example, a culture medium and an oxygen scavenger are accommodated in the tubular container 12 of the package 10, and a certain amount of purified water is sealed in the internal package 16. Such an inspection tool is used as follows when anaerobic culture of microorganisms is performed.

  After removing the cap 14 from the opening of the tubular container 12 of the package 10 and inoculating anaerobic bacteria on the medium in the tube-shaped container 12, the cap 14 is fitted into the opening of the tubular container 12 and the package 10 is sealed. Subsequently, the tubular container 12 of the package 10 is pushed and deformed with a finger, and the inner package 16 is ruptured by applying a force to the inner package 16 so that purified water flows out from the inner package 16. . As a result, the purified water that has flowed out of the inner package 16 reacts with the oxygen scavenger in the tubular container 12 to remove oxygen from the sealed package 10, and the interior of the package 10 is anaerobic. Thus, anaerobic culture is performed on the medium in the package 10. Thus, when this test tool is used, a test medium in an anaerobic atmosphere can be prepared easily and in a short time.

  In addition, Hana tetrathionate medium, a selective enrichment medium for Salmonella spp., Is added with a certain amount of iodine solution (aqueous solution of iodine and potassium iodide) to Hana tetrathionate basic medium before the culture test. Then, thiosulfate, which is a component in the basal medium, is reacted with iodine and potassium iodide to produce potassium tetrathionate, but potassium tetrathionate is unstable in an aqueous solution. For this reason, it is necessary to perform inspection on the day when potassium tetrathionate is produced. However, if the inspection tool having the configuration shown in FIG. 1 is used, the Hana tetrathionate medium can be easily and shortly used immediately before the culture inspection. Can be prepared in time. That is, Hana tetrathionate basal medium is placed in the tube-shaped container 12 of the package 10, and a certain amount of iodine solution is sealed in the internal package 16. And before an inspection, as mentioned above, the tube-shaped container 12 of the package 10 is pushed with a finger, the internal package 16 is ruptured, and the iodine solution is caused to flow out from the internal package 16. As a result, iodine and potassium iodide of the iodine solution flowing out from the inner package 16 react with thiosulfate in the basal medium in the tube-like container 12 to produce potassium tetrathionate, The Hana tetrathionate medium containing is completed.

  As for the culture medium, the expiration date of the powder culture medium is usually longer than that of the liquid culture medium. Therefore, in the inspection tool having the configuration shown in FIG. 1, sterilized purified water is put into the tube-shaped container 12 of the package 10, and a predetermined amount of powder medium and air are sealed in the inner package 16. If it does in this way, the tube-shaped container 12 of the package 10 will be pushed with a finger, the internal package 16 will be ruptured, the powder culture medium in the internal package 16 will be dissolved in sterilized purified water, and a liquid medium will be completed just before use. Thus, the expiration date of the medium can be set longer. In addition, a powder culture medium may be put in the tube-shaped container 12 of the package 10, and sterilized purified water may be sealed in the internal package 16. Further, among the components of the liquid medium, a component having a short expiration date in the solution state, for example, a powdered material such as an antibiotic or a pigment that is decomposed by light, is enclosed in the inner package 16, and the package 10 has a tube shape. A solution containing other components of the liquid medium may be placed in the container 12.

  Further, when the concentrated medium is sealed in the inner package 16 and the diluent is put in the tube-shaped container 12 of the package 10, the tube-shaped container 12 of the package 10 is pushed with a finger and the inner package is packed. When the body 16 is ruptured, the concentrated medium that has flowed out of the inner package 16 is diluted with a diluent, and a liquid medium having a predetermined concentration is obtained. In this case, as shown in FIG. 2, if the cotton swab 26 is attached to the inside of the cap 24 of the package 22, the inspection tool can be used for the following wiping inspection.

  For example, 10 ml of the diluent referred to in the food hygiene test is placed in the tubular container 12 of the package 22, and the cotton ball 28 of the cotton swab 26 is immersed in the diluted solution. 1 ml of LMX medium having a 10-fold concentration (shows blue when E. coli is present, and emits blue when irradiated with UV light having a wavelength of 344 nm) is enclosed. Then, when performing a wiping test, the cap 24 is removed from the opening of the tubular container 12, and the surface of the site to be tested is wiped with a cotton ball 28 of a cotton swab 26 in a wet state with a diluent, and a sample is collected. After the sample is collected, the cotton ball 28 of the cotton swab 26 to which the sample is attached is inserted into the tubular container 12, and the cap 24 is fitted into the opening of the tubular container 12 to seal the package 22. Thereafter, the tube-shaped container 12 of the package 22 is pushed with a finger to rupture the inner package 16, and the LMX medium having a 10-fold concentration is diluted 10-fold with a diluent to complete the LMX medium having a desired concentration. And bacteria are culture | cultivated with a LMX culture medium in the package 22 in the state. By doing in this way, while being able to perform a series of operation from sample collection in the wiping inspection to culture | cultivation of bacteria efficiently and in a short time, the effort of medium preparation can be saved.

  Furthermore, a disinfectant such as alcohol is enclosed in the tube-shaped container 12 of the above-described package 22, and another internal package that is ruptured by an external force similarly to the internal package 16 may be accommodated. . With such a configuration, after the bacteria are cultured in the liquid medium and the inspection is completed, the outer surface of the tubular container 12 is pushed with a finger to rupture another inner package, and the disinfectant from the inner package The disinfectant is mixed with the medium in which Escherichia coli and the like are propagated. Thereby, since the culture medium can be sterilized, it becomes possible to safely discard the inspection tool in which the culture medium is stored after the wiping test.

  In the above-described embodiment, the test tool that can complete the test medium before use has been described. However, the first and second contents are changed by the same configuration as the test tool shown in FIG. Thus, an inspection tool that can be used for the following applications can be provided.

  A non-selective medium for growing the target bacteria and various germs is put in the tube-shaped container 12 of the package 10, and a selective agent that suppresses the growth of germs other than the target bacteria is enclosed in the internal package 16. To do. When the target bacteria are present only in a small amount in the specimen or when the target bacteria are damaged, first, the bacteria are cultured in the non-selective medium in the tubular container 12 of the package 10. By carrying out the above, the target bacteria and miscellaneous bacteria are propagated. Thus, for example, after about 6 hours, after the growth of a small amount of target bacteria and recovery of damaged bacteria, the tube-shaped container 12 of the package 10 is pushed with a finger to rupture the internal package 16 as described above. Then, the selective agent is caused to flow out from the inner package 16. Thereby, a selective agent is added to the non-selective medium in the package 10, and in the subsequent culture, the target bacteria grow while suppressing the growth of various bacteria other than the target bacteria. Therefore, after culturing using the non-selective medium, the same purpose can be achieved in the tubular shape of the package 10 without inoculating a part of the selective medium or adding a selective agent to the non-selective medium. This can be achieved by a simple operation in which the inner package 16 is ruptured by pushing the container 12 with a finger.

  In addition, a culture medium is placed in the tube-shaped container 12 of the package 10, and an inspection pigment such as phenol red or methyl red is enclosed in the internal package 16. Then, in the tube-shaped container 12 of the package 10, after the microorganisms are grown in the medium as usual, the tube-shaped container 12 of the package 10 is pushed with a finger to rupture the internal package 16, and the internal package 16 The test dye is allowed to flow out of the medium, and the test dye is added to the medium. This allows microorganisms to grow quickly and reliably in a medium that does not contain pigments that inhibit the growth of microorganisms, and then observes color changes in the medium to which pigments have been added. Changes in potential and the like can be sensed.

  Next, FIG. 3 is a partially broken perspective view showing an inspection tool having a configuration different from the above-described inspection tool. This inspection tool is for inspecting the presence or absence of contaminants such as proteins and starch that cause microbial contamination. An internal package 36 is provided inside a package 30 composed of a tubular container 32 and a cap 34. The configuration in which the cotton swab 38 is accommodated inside the cap 34 and the inner package 36 is ruptured by the deformation force applied to the package 30 is the same as the inspection tool shown in FIG. It is the same. This inspection tool is different from the above-described inspection tool in that the contents are not accommodated in the tubular container 32 of the package 30. In the inner package 36, a reagent that reacts with a pollutant such as protein or starch that causes microbial contamination is enclosed.

  When the wiping inspection is performed using the inspection tool shown in FIG. 3, the cap 34 is removed from the opening of the tubular container 32 of the package 30, and the inspection is performed with the cotton ball 40 of the cotton swab 38 attached to the cap 34. Remove the sample by wiping the surface of the area. After the sample is collected, the cotton ball 40 of the cotton swab 38 to which the sample is attached is inserted into the tube-shaped container 32, and the cap 24 is fitted into the opening of the tube-shaped container 32, and then the tube-shaped container 32 of the package 30 is pointed. The inner package 36 is ruptured by pushing to cause the reagent to flow out from the inner package 36. The reagent that has flowed out of the inner package 36 is applied to the cotton ball 40 of the cotton swab 38 and reacts with the contaminant agent adhering to the cotton ball 40. Thereby, the presence or absence of the pollutant can be inspected. Thus, when this inspection tool is used, nutritional components such as protein and starch that cause microbial contamination remain after washing utensils, cooking tables, dishes, etc. in food factories and restaurants. It can be inspected promptly to see if it is not. Before use of the inspection tool, the swab 26 is accommodated in the sealed package 30, and the reagent accommodated in the tubular container 32 together with the swab 26 is enclosed in the internal package 36. It is in the state. For this reason, since the cotton ball 40 of the cotton swab 38 is kept in a clean state without being stained with the reagent, a reliable inspection can be performed.

  In the above-described embodiment, the tubular containers 12, 32 of the packaging bodies 10, 22, and 30 are formed of a flexible resin material, but the material of the tubular container is not particularly limited. Moreover, even if the whole tube-shaped container does not have a softness | flexibility, a part should just have a softness | flexibility or flexibility. Furthermore, in the said embodiment, although the package bodies 10, 22, and 30 comprised the tubular containers 12 and 32 and the caps 14, 24, and 34, you may make it use the bag which can be sealed instead of a container. Moreover, the number of the internal packaging bodies 16 and 36 accommodated in the packaging bodies 10, 22 and 30 is not limited to one and may be two or more. Further, the inner package is not limited to a bag shape formed of a film material and a part of the peripheral edge is weakly bonded as in the above-described embodiment, but is also a sealed container that is ruptured by an external force applied to the package. It may be. Moreover, you may make it replace the content put into the package 10, 22, 30 and the internal package 16, 36 mutually with the thing of the said embodiment.

It is a perspective view which shows one example of embodiment of this invention and shows the state which fractured | ruptured some inspection tools. It is a perspective view of the inspection tool which shows another embodiment of this invention. FIG. 3 is a partially broken perspective view showing an inspection tool having a configuration different from those in FIGS. 1 and 2.

Explanation of symbols

10, 22, 30 Package 12, 32 Tubular container 14, 24, 34 Cap 16, 36 Inner package 20 First contents 26, 38 Cotton swab 28, 40 Cotton swab ball

Claims (13)

A liquid or powdery first content is accommodated in a package having at least a part of flexibility or flexibility, and a test medium is prepared by mixing with the first content. One or two or more internal packaging bodies that contain liquid, powdery or gaseous second contents enclosed and ruptured by a deformation force applied to the packaging body from the outside are accommodated, and the packaging body is sealed. Inspection tool characterized by The test tool according to claim 1, wherein the first content is a medium and an oxygen scavenger, the second content is purified water, and a test medium for anaerobic culture is prepared. 2. The test medium according to claim 1, wherein the first content is a Hana tetrathionate basal medium, the second content is an iodine solution, and a test medium that is a selective enrichment medium of Salmonella is prepared. Inspection tool. The test tool according to claim 1, wherein the first content is sterilized purified water, the second content is a powder medium, and a test medium that is a liquid medium is prepared. The second content is a powdered part of the components of the liquid medium, and the first content is a solution containing other components of the liquid medium, and the test medium is a liquid medium. The inspection tool according to claim 1 to be prepared. The test tool according to claim 1, wherein the second content is a concentrated medium, the first content is a diluent, and a test medium that is a liquid medium is prepared. The package is composed of a tube-shaped container formed of a flexible resin material and provided with an opening, and a cap that is detachably fitted into the opening of the tube-shaped container. The inspection tool according to claim 6, wherein a swab for wiping inspection is attached, and a cotton ball of the swab is immersed in the diluent. The inspection tool according to claim 7, wherein an inner package that encloses a disinfectant and is ruptured by a deformation force applied to the package from the outside is further accommodated in the package. One or two or more inner packagings that contain a culture medium inside a package that is at least partially flexible or flexible, and that are encapsulated with additives and that burst due to deformation force applied from the outside to the package An inspection tool characterized by containing a body and sealing the package. The test tool according to claim 9, wherein the culture medium is a non-selective medium for growing target bacteria and various germs, and the additive is a selective agent that suppresses the growth of various germs other than the target bacteria. The inspection tool according to claim 9, wherein the additive is an inspection pigment. It is composed of a container having at least a part of flexibility or flexibility and provided with an opening, and a cap that is detachably fitted into the opening of the container, and is used for wiping inspection inside the cap. A deforming force that is applied to the packaging body from the outside, in which a reagent that reacts with a pollutant substance that causes microbial contamination is sealed inside the packaging body in which the cotton swab is attached and the cotton swab is inserted into the container One or two or more internal packaging bodies which burst by this are accommodated, The said packaging body was sealed, The inspection tool characterized by the above-mentioned. The inspection tool according to any one of claims 1 to 12, wherein the inner package is formed of a film material in a sealed bag shape and at least a part of a peripheral edge thereof is weakly bonded.

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