JP2006204286A - Freeze-fermented material dough, bread dough using the same, method for producing the bread dough, and bread using the bread dough - Google Patents

Freeze-fermented material dough, bread dough using the same, method for producing the bread dough, and bread using the bread dough Download PDF

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JP2006204286A
JP2006204286A JP2005196104A JP2005196104A JP2006204286A JP 2006204286 A JP2006204286 A JP 2006204286A JP 2005196104 A JP2005196104 A JP 2005196104A JP 2005196104 A JP2005196104 A JP 2005196104A JP 2006204286 A JP2006204286 A JP 2006204286A
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dough
fermented
bread
seed
frozen
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JP4482495B2 (en
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Akinori Nakamura
昭典 中村
Akira Kanzaki
朗 神崎
Ken Kainuma
謙 貝沼
Masafumi Goto
雅文 後藤
Yoshio Sakai
美穂 酒井
Biho Takahashi
美峰 高橋
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Yamazaki Baking Co ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a liquid fermented material easy to handle in its distribution/storage. <P>SOLUTION: A liquid fermented material is mixed with at least wheat flour to prepare nonfluid fermented material dough, which is then frozen. Thereby, adverse effects in distribution/storage involved in conventional liquid fermented materials can be eliminated without costing a lot of money, enabling frozen fermented material dough easy to handle in its distribution/storage to be obtained. Further, bread dough incorporated with the fermented material dough is good in spreadability similarly to bread dough incorporated with conventional liquid fermented material dough. Furthermore, bread produced using the above fermented material dough is improved in flavor compared to bread produced by incorporating conventional liquid fermented materials and has the effect of deterioration retardancy. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

本発明は、冷凍醗酵種生地、該冷凍醗酵種生地を用いたパン生地、及び該パン生地を用いたパン、並びにこれらの製造方法に関する。より具体的には、液状醗酵種に少なくとも小麦粉を混合して作成した醗酵種生地を冷凍した冷凍醗酵種生地、該冷凍醗酵種生地を用いたパン生地、及び該パン生地を用いたパン、並びにこれらの製造方法に関する。   The present invention relates to a frozen fermented seed dough, a bread dough using the frozen fermented seed dough, a bread using the dough, and a method for producing the same. More specifically, a frozen fermented seed dough obtained by freezing a fermented seed dough prepared by mixing at least flour with a liquid fermented seed, a bread dough using the frozen fermented seed dough, a bread using the bread dough, and these It relates to a manufacturing method.

従来より、パンの風味改善やパン生地の製パン性向上を目的として、醗酵種が用いられているが、従来の醗酵種は、液状でパン生地の製造に用いられていた(特許文献1参照)。しかし、常温で液状の醗酵種を流通および保管する場合、醗酵種の変敗、醗酵過多、或いは活性の喪失等のおそれがあるため、冷蔵温度帯で流通および保管する必要があった。しかしながら、冷蔵温度帯での流通、保管には、高額の費用がかかるのはもちろん、冷蔵温度帯であっても、上述の弊害を完全に防止することはできないため、長期間に渡る流通、保管は不可能であった。   Conventionally, fermented seeds have been used for the purpose of improving the flavor of bread and improving the bread-making properties of bread dough. Conventional fermented seeds have been used in the production of bread dough in liquid form (see Patent Document 1). However, when liquid fermented seeds are circulated and stored at room temperature, there is a risk of deterioration of the fermented seeds, excessive fermentation, loss of activity, and the like, so it has been necessary to distribute and store them in a refrigerated temperature zone. However, distribution and storage in the refrigeration temperature zone are expensive, and even in the refrigeration temperature zone, the above-mentioned adverse effects cannot be completely prevented, so distribution and storage over a long period of time. Was impossible.

このような理由から、醗酵種は凍結乾燥して用いられるのが一般的であった(特許文献2参照)。しかし、凍結乾燥させた醗酵種には、醗酵種本来の風味が減少したり、高額の費用がかかるといった問題があった。また、凍結乾燥技術は通常の製パンメーカーには無い特殊な技術であり、製パンメーカーが独自に醗酵種の凍結乾燥物を製造することができないため、専門の業者に製造を頼らざるを得ず、製パンメーカーが納得できる性質を有する醗酵種の凍結乾燥物を得ることが困難であった。   For these reasons, the fermented species are generally lyophilized before use (see Patent Document 2). However, freeze-dried fermented seeds have problems such as a decrease in the original flavor of the fermented seeds and high costs. In addition, freeze-drying technology is a special technology that is not found in ordinary bread makers, and since bread makers cannot independently produce freeze-dried fermented seeds, they must rely on specialized suppliers for production. Therefore, it was difficult to obtain a freeze-dried fermented seed having properties that can be satisfied by a bread maker.

なお、サワーブレッドの製造方法として、液状の乳酸菌醗酵種をパンの第一次原料粉に添加混合し、イースト菌不存在下で乳酸醗酵のみ先行させ、これに第二次原料粉、イースト、その他の原料を加えてパン生地として、その後はイースト醗酵を主とする醗酵工程等、常法通りの工程により製造するものがある(特許文献3参照)。しかし、ここで用いられる液状醗酵種は、生米等の乳酸菌醗酵物を磨砕して得られた特殊なものであって、しかも、先行させる乳酸醗酵に長時間を要するものである。   In addition, as a method for producing sour bread, liquid lactic acid bacteria fermented seeds are added to and mixed with the primary raw powder of bread, and only lactic acid fermentation is preceded in the absence of yeast. There are some which are made by adding a raw material to make bread dough, and thereafter by a conventional process such as a fermentation process mainly consisting of yeast fermentation (see Patent Document 3). However, the liquid fermentation species used here are special ones obtained by grinding a lactic acid bacteria fermentation product such as raw rice, and require a long time for the lactic acid fermentation to precede.

また、冷凍パン生地を用いるパンの製造方法として、所定のパン生地原料に製パン用酵母を含まない状態でラクトバチルス・パネックス等の乳酸菌醗酵生成物を添加・混合して、冷凍し、これを解凍した後製パン用酵母を添加、混合し、醗酵させて焼成するものがある(特許文献4参照)。これは、まず、イースト以外のパン生地原料で冷凍生地を作成しておいて、冷凍生地解凍後、イーストを添加、混合するものであり、本発明とは全く別の技術である。
特公昭57−17496号公報 特許第2766374号公報 特許第3180900号公報 特開2003−79307号公報
In addition, as a method of producing bread using frozen bread dough, lactic acid fermentation products such as Lactobacillus panex and the like were added to and mixed with a predetermined bread dough raw material in a state not containing yeast for baking, and then thawed. There exists what adds the yeast for post-production bread, mixes, ferments, and bakes (refer patent document 4). This is a technique completely different from the present invention, in which a frozen dough is first made from a bread dough material other than yeast, and after the frozen dough is thawed, yeast is added and mixed.
Japanese Patent Publication No.57-17496 Japanese Patent No. 2766374 Japanese Patent No. 3180900 JP 2003-79307 A

上述の通り、従来の液状醗酵種の流通・保管は、変敗、醗酵過多、活性喪失等を防止するため、冷蔵温度帯での流通・保管の必要があった。しかし、冷蔵温度帯での流通および保管には多額の費用がかかるとともに、冷蔵温度でも上述の弊害は完全に防止できないため、長期の流通・保管は不可能であった。従って、一般的には、凍結乾燥させた醗酵種が用いられていたが、凍結乾燥させた醗酵種は高額で、しかも醗酵種本来の風味が減少してしまう、等の問題があった。   As described above, the distribution and storage of the conventional liquid fermentation species required distribution and storage in a refrigerated temperature zone in order to prevent deterioration, excessive fermentation, loss of activity, and the like. However, distribution and storage in the refrigeration temperature zone is expensive, and the above-mentioned adverse effects cannot be completely prevented even at the refrigeration temperature, so long-term distribution and storage is impossible. Therefore, generally, freeze-dried fermented seeds have been used, but freeze-dried fermented seeds are expensive, and the original flavor of the fermented seeds is reduced.

本発明は、上述の従来技術の欠点を解消すべくなされたもので、流通・保管時の取り扱いが容易な冷凍醗酵種生地を提供するものである。また、本発明は、従来の液状醗酵種を添加したパン生地と同様の生地物性を有するパン生地を提供するものである。さらに、本発明は、従来の液状醗酵種を添加して製造されたパンよりも風味が向上した、老化遅延効果を有するパンを提供するものである。   The present invention has been made to eliminate the above-described drawbacks of the prior art, and provides a frozen fermented seed dough that is easy to handle during distribution and storage. Moreover, this invention provides the bread dough which has the same dough physical property as the bread dough which added the conventional liquid fermentation seed | species. Furthermore, this invention provides the bread | pan which has the aging delay effect which the flavor improved rather than the bread | pan produced by adding the conventional liquid fermentation seed | species.

本発明者らは、従来の技術に関する上記課題を解決すべく、鋭意研究を行った結果、本発明を完成した。すなわち、本発明は以下の事項に関する。
(1)液状醗酵種に少なくとも小麦粉を混合して非流動状の醗酵種生地を作成し、冷凍することを特徴とする冷凍醗酵種生地。
(2)前記混合して作成した醗酵種生地を、極力醗酵させないように、速やかに冷凍することを特徴とする(1)に記載の冷凍醗酵種生地。
(3)前記混合して作成した醗酵種生地を、分割及び丸め整形してから、冷凍することを特徴とする(1)又は(2)に記載の冷凍醗酵種生地。
(4)前記液状醗酵種が、少なくとも、小麦粉、水並びに乳酸菌及び/又はイーストから構成されることを特徴とする、(1)乃至(3)のいずれか一項に記載の冷凍醗酵種生地。
(5)前記液状醗酵種が、醗酵種として直ちにパンの製造に使用できる程度に醗酵させたものであることを特徴とする、(1)乃至(4)のいずれか一項に記載の冷凍醗酵種生地。
(6)前記液状醗酵種が、糖類を含むことを特徴とする、(1)乃至(5)のいずれか一項に記載の冷凍醗酵種生地。
(7)前記液状醗酵種が、種継ぎをしたものであることを特徴とする、(1)乃至(6)のいずれか一項に記載の冷凍醗酵種生地。
(8)前記液状醗酵種が、該液状醗酵種を構成する小麦粉に対し50〜150質量%の水から作成されることを特徴とする、(4)乃至(7)のいずれか一項に記載の冷凍醗酵種生地。
(9)前記醗酵種生地が、前記液状醗酵種に対して40〜50質量%の小麦粉を混合して作成することを特徴とする、(1)乃至(8)のいずれか一項に記載の冷凍醗酵種生地。
(10)前記(1)乃至(9)のいずれか一項に記載の冷凍醗酵種生地を解凍してからパン生地の混捏工程で添加することを特徴とするパン生地。
(11)前記冷凍醗酵種生地を、前記パン生地を構成する小麦粉に対して3〜30質量%添加することを特徴とする、(10)に記載のパン生地。
(12)前記(10)又は(11)に記載のパン生地を焼成することを特徴とするパン。
(13)前記(1)乃至(9)のいずれか一項に記載の冷凍醗酵種生地を解凍してからパン生地の混捏工程で添加することを特徴とするパン生地の製造方法。
(14)前記冷凍醗酵種生地を、前記パン生地を構成する小麦粉に対して3〜30質量%添加することを特徴とする、(13)に記載のパン生地の製造方法。
(15)前記(13)又は(14)に記載の方法により製造されたパン生地を焼成することを特徴とするパンの製造方法。
The inventors of the present invention have completed the present invention as a result of intensive studies in order to solve the above-described problems related to the prior art. That is, the present invention relates to the following matters.
(1) A frozen fermented seed dough comprising mixing a liquid fermented seed with at least wheat flour to create a non-fluid fermented seed dough and freezing.
(2) The frozen fermented seed dough according to (1), wherein the fermented seed dough prepared by mixing is rapidly frozen so as not to ferment as much as possible.
(3) The frozen fermented seed dough according to (1) or (2), wherein the fermented seed dough prepared by mixing is frozen after being divided and rounded.
(4) The frozen fermented seed dough according to any one of (1) to (3), wherein the liquid fermented seed is composed of at least flour, water, lactic acid bacteria and / or yeast.
(5) The frozen fermentation according to any one of (1) to (4), wherein the liquid fermentation species is fermented to such an extent that it can be used immediately for bread production as a fermentation species. Seed dough.
(6) The frozen fermented seed dough according to any one of (1) to (5), wherein the liquid fermented seed contains a saccharide.
(7) The frozen fermented fermented seed dough according to any one of (1) to (6), wherein the liquid fermented seed is seeded.
(8) The liquid fermented seed is produced from 50 to 150% by mass of water with respect to the wheat flour constituting the liquid fermented seed, described in any one of (4) to (7). Frozen fermented seed dough.
(9) The fermented seed dough is prepared by mixing 40 to 50% by weight of flour with respect to the liquid fermented seed, according to any one of (1) to (8). Frozen fermented seed dough.
(10) A bread dough, which is added in a kneading step of bread dough after thawing the frozen fermented seed dough according to any one of (1) to (9).
(11) The bread dough according to (10), wherein 3 to 30% by mass of the frozen fermented seed dough is added to the flour constituting the bread dough.
(12) A bread characterized by baking the bread dough according to (10) or (11).
(13) A method for producing bread dough, comprising thawing the frozen fermented seed dough according to any one of (1) to (9) above and then adding the dough in a kneading step of bread dough.
(14) The method for producing bread dough according to (13), wherein 3 to 30% by mass of the frozen fermented seed dough is added to the flour constituting the bread dough.
(15) A method for producing bread, comprising baking the dough produced by the method according to (13) or (14).

本発明により、従来の液状醗酵種が有する流通・保管上の弊害を高額な費用をかけることなく解消して、流通・保管時の取り扱いが容易な冷凍醗酵種生地が得られた。本発明の醗酵種生地を添加したパン生地は、従来の液状醗酵種生地を添加したパン生地同様、伸展性の良好なものだった。該醗酵種生地を用いたパンは、従来の液状醗酵種を添加して製造されたパンよりも、風味が向上し、老化遅延効果を有するものだった。   According to the present invention, a frozen fermented fermented dough that can be easily handled during distribution and storage can be obtained by eliminating the adverse effects on distribution and storage of conventional liquid fermented seeds without expensive costs. The bread dough to which the fermented seed dough of the present invention was added was excellent in extensibility as the bread dough to which the conventional liquid fermented seed dough was added. The bread using the fermented seed dough had a flavor improvement and an aging delay effect as compared with bread produced by adding conventional liquid fermented seeds.

発明を実施するための形態BEST MODE FOR CARRYING OUT THE INVENTION

はじめに、本発明の冷凍醗酵種生地は、液状醗酵種に少なくとも小麦粉を混合して非流動状の醗酵種生地を作成し、冷凍したものである。
本発明において、液状醗酵種とは、流動性があり、かつ保形性および可塑性に欠ける性状の醗酵種を意味し、少なくとも小麦粉、水並びに乳酸菌及び/又はイーストから構成されるものである。
First, the frozen fermented seed dough of the present invention is prepared by mixing a liquid fermented seed with at least wheat flour to produce a non-fluid fermented seed dough.
In the present invention, the liquid fermentation species means a fermentation species having fluidity and lacking in shape retention and plasticity, and is composed of at least flour, water, lactic acid bacteria and / or yeast.

該液状醗酵種を構成する小麦粉としては、強力粉、中力粉、薄力粉、荒挽粉、全粒粉、デュラム粉等を使用することができ、複数種の小麦粉を併用することもできる。
本発明において、液状醗酵種を構成する乳酸菌及び/又はイーストを添加する形態としては、乳酸菌及び/又はイーストからなる、又はこれを含む単離培養菌体(例えば、集菌した菌を寒天培地で培養して複数のコロニーを得、それぞれのコロニーを寒天培地での培養を繰り返すことにより得られる菌体)、醗酵液、醗酵物、これらの乾燥粉末、元種、その他のうち1種又は2種以上を添加する形態のようにスターターとしての乳酸菌及び/又はイーストそのものを人為的に添加することであるが、これには限られず、小麦粉やライ麦粉等の小麦粉以外の穀粉を添加することに伴い、これらの穀粉に付着している自然菌を液状醗酵種中に取り込むことにより、このような自然菌を利用することをも包含する。
As wheat flour constituting the liquid fermented seed, strong flour, medium strength flour, weak flour, rough ground flour, whole grain flour, durum flour and the like can be used, and a plurality of types of wheat flour can be used in combination.
In the present invention, the form of adding lactic acid bacteria and / or yeast constituting the liquid fermentation species is an isolated cultured microbial cell consisting of or containing lactic acid bacteria and / or yeast (for example, collected bacteria in an agar medium). A plurality of colonies are obtained by culturing, and each colony is obtained by repeating culture on an agar medium), fermented liquid, fermented product, dry powder of these, original species, or other one or two of them It is to artificially add lactic acid bacteria and / or yeast itself as a starter as in the form of adding the above, but is not limited to this, and with adding flour other than wheat flour such as wheat flour and rye flour It also encompasses the use of such natural bacteria by incorporating the natural bacteria adhering to these flours into the liquid fermented species.

ここで、本明細書において「種起こし」とは、最初の醗酵種(当業界において、初種、親種、一番種などと呼ばれることがあり、本明細書においては初種ともいう)の調製を意味する。さらに、本明細書において「種継ぎ」とは、種起こしした初種を用いた醗酵種の調製と、種継ぎした醗酵種を用いた1回又は複数回の醗酵種の調製とのそれぞれを含む意味である。具体的には、例えば、種継ぎを行わないで、醗酵種を調製する場合、初めの種起こしのみを行って醗酵種を調製することになる。また、種起こし及び種継ぎを合計5回行って醗酵種を調製する場合、初めの種起こしを行った後に、種継ぎを4回行うことになる。さらに、種起こし及び種継ぎを合計10回行って醗酵種を調製する場合、初めの種起こしを行った後に、種継ぎを9回行うことになる。   Here, the “seed raising” in the present specification refers to the first fermented species (sometimes referred to as the first species, the parent species, the first species, etc. in the industry, also referred to as the first species in the present specification). Means preparation. Further, in the present specification, “seeding” includes preparation of a fermented species using the seeded initial species and preparation of one or more fermented species using the inherited fermented species. Meaning. Specifically, for example, when preparing a fermented seed without performing seeding, only the first seeding is performed to prepare the fermented seed. Moreover, when preparing a fermented seed by performing seeding and seeding a total of 5 times, after performing initial seeding, seeding is performed 4 times. Further, when preparing the fermented seed by performing seeding and seeding a total of 10 times, the seeding is performed 9 times after the initial seeding.

より具体的には、最初の醗酵種を調製する場合(すなわち、種起こしする場合)、乳酸菌及び/又はイーストを添加する形態としては、乳酸菌及び/又はイーストからなる、又はこれを含む単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他のうちの1種又は2種以上を添加してもよく、また、このような乳酸菌及び/又はイーストそのものを添加することなく、醗酵種を構成する小麦粉や小麦粉以外の穀粉に付着している自然菌を利用することもできる。   More specifically, when preparing the first fermentation species (that is, when inoculating), as a form to which lactic acid bacteria and / or yeast are added, an isolated culture consisting of or containing lactic acid bacteria and / or yeasts One or two or more of bacterial cells, fermentation broth, fermented products, dry powders thereof, etc. may be added, and fermented species without adding such lactic acid bacteria and / or yeast itself. Natural fungi adhering to flour other than wheat flour and wheat flour can also be used.

また、初種を基礎として(元種として)1回、又は初種から出発して(初種の少なくとも一部を最初の元種として)複数回、若しくは繰り返し無数に種継ぎを行う場合、それぞれの種継ぎにおいて、乳酸菌及び/又はイーストを添加する形態として、元種に含有される乳酸菌及び/又はイーストや、種継ぎの際に添加する醗酵種を構成する小麦粉や小麦粉以外の穀粉に付着している自然菌を利用することができ、さらに必要に応じて、前記単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他のうちの1種又は2種以上を添加することもできる。   In addition, when performing seeding on the basis of the first species once (as the original species), or starting from the first species a plurality of times (at least a part of the first species as the first original species), or repeatedly infinitely, As a form of adding lactic acid bacteria and / or yeast, the lactic acid bacteria and / or yeast contained in the original species and the flour other than wheat flour and wheat flour constituting the fermented seed added at the time of seeding are attached. Natural bacteria can be used, and if necessary, one or more of the above-mentioned isolated cultured cells, fermentation liquid, fermentation product, these dry powders, etc. may be added. it can.

種起こしにおいても、種継ぎにおいても、前記単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他の乳酸菌及び/又はイーストそのものを添加しない場合には、通常、菌の増殖を図るため、比較的長時間の醗酵が必要とされる。   In both seeding and seeding, if the isolated cultured cells, fermentation broth, fermented product, dried powder thereof, other lactic acid bacteria and / or yeast itself are not added, the bacteria are usually grown. Therefore, fermentation for a relatively long time is required.

例えば、単離培養菌体などの乳酸菌及び/又はイーストそのものを添加しなければ、必要時間醗酵させたとしても、必要な、又は十分な菌の増殖を図ることができないことがある。この場合、単離培養菌体などの乳酸菌及び/又はイーストそのものを添加することにより、増殖菌数の不足分を補足することができる。この場合、通常、著しく長時間の醗酵を必要としない。   For example, if a lactic acid bacterium such as an isolated cultured microbial cell and / or yeast itself is not added, necessary or sufficient growth of the bacterium may not be achieved even if fermentation is performed for a necessary time. In this case, the deficiency of the number of proliferating bacteria can be supplemented by adding lactic acid bacteria such as isolated cultured cells and / or yeast itself. In this case, it is usually not necessary to perform a significantly long fermentation.

従って、本発明において、種起こし及び/又は種継ぎで前記単離培養菌体その他の乳酸菌及び/又はイーストそのものを添加するとしても、液状醗酵種中の菌数、醗酵条件、菌の増殖の早さ、求める最終醗酵状態等の事情に応じて、種起こし時だけに添加してもよいし(種継ぎには添加しない)、これに対して、種起こし時には添加せずに、種継ぎだけに添加するようにしてもよい。また、種起こし及び種継ぎの両者で添加するようにしてもよい。さらには、種継ぎで添加する際も、それぞれの種継ぎごとに毎回添加するようにしてもよいし、種継ぎの1回おき、2回おき、3回おき、というように間隔をあけて定期的に添加してもよく、若しくは、任意に不規則的に時々添加してもよい。   Therefore, in the present invention, even if the isolated cultured cells and other lactic acid bacteria and / or yeast itself are added by seeding and / or seeding, the number of bacteria in the liquid fermentation species, fermentation conditions, and rapid growth of the bacteria. Depending on circumstances such as the final fermentation state to be sought, it may be added only at the time of seeding (not added to seeding), but it is not added at the time of seeding but only for seeding. You may make it add. Moreover, you may make it add by both seed raising and seed joining. Furthermore, when adding by seed joining, it may be added every time each seed joining, or at regular intervals at intervals such as every second, every third, etc. Or may be added randomly at times.

同じく乳酸菌の菌種としては、本発明の目的・効果を妨げるものでなければ、特に制限はなく、いずれのものも使用できる。好適には、グルコース等の糖類から多量の乳酸を生成する菌で、ラクトバチルス属、ロイコノストック属、ストレプトコッカス属、ペディオコッカス属等に属するものである。具体的には、例えば、ラクトバチルス・プランタラム、ラクトバチルス・アシドフィラス、ラクトバチルス・ヘルベティカス、ラクトバチルス・ブレビス、ラクトバチルス・ブルガリカス、ラクトバチルス・サンフランシスコ、ラクトバチルス・イタリカス、ラクトバチルス・ヒルガルディ、ラクトバチルス・カゼイ、ラクトバチルス・ファーメンタム、ラクトバチルス・デルブルッキー、ラクトバチルス・ライヒマニ、ラクトバチルス・パストリアヌス、ラクトバチルス・ブヒネリ、ラクトバチルス・フルクティボランス、ラクトバチルス・ルテリ、ラクトバチルス・パラアリメンタリウス、ロイコノストック・オイアノス、ロイコノストック・デキストラニクム、ロイコノストック・クレモリス、ストレプトコッカス・サーモフィラス、ストレプトコッカス・クレモリス、ストレプトコッカス・ラクティス、ストレプトコッカス・ジアセチラクチス、ストレプトコッカス・サリバリウス、ストレプトコッカス・フェカリス、ペディオコッカス・ペントサシウス、ペディオコッカス・ハロフイラス、ペディオコッカス・アシディラクティシ、エンテロコッカス・フェカリス、テトラゲノコッカス・ハロフィラス、ラクトコッカス・ラクティス等が挙げられる。これらの乳酸菌を単独で、又は複数種を組み合わせて使用することができる。   Similarly, the bacterial species of lactic acid bacteria are not particularly limited as long as they do not interfere with the purpose and effect of the present invention, and any of them can be used. Preferably, it is a bacterium that produces a large amount of lactic acid from sugars such as glucose, and belongs to the genus Lactobacillus, Leuconostoc, Streptococcus, Pediococcus and the like. Specifically, for example, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus san francisco, Lactobacillus italica, Lactobacillus hilgardi, lacto Bacillus casei, Lactobacillus fermentum, Lactobacillus delbruecki, Lactobacillus reichmanni, Lactobacillus pastorianus, Lactobacillus buhineri, Lactobacillus frucutivorans, Lactobacillus luteri, Lactobacillus paraalimentarius , Leuconostok Oianos, Leuconostok Dextranicum, Leuconostok Cremoris, Streptococcus thermophilus, Streptococcus cremoris, Streptococcus lactis, Streptococcus diacetilactis, Streptococcus salivalius, Streptococcus faecalis, Pediococcus pentosasius, Pediococcus halofilus, Pediococcus atelodiococcus -Halophilus, Lactococcus lactis, etc. These lactic acid bacteria can be used alone or in combination.

同じくイーストの菌種としては、本発明の目的・効果を妨げるものでなければ、特に制限はなく、いずれのものも使用できる。具体的には、例えば、サッカロミセス・セレビシエ、サッカロミセス・ロゼイ、サッカロミセス・クルバータス、サッカロミセス・シュバリエリ、サッカロミセス・フロレンティナス、サッカロミセス・エグジグアス、サッカロミセス・バイリイ、クルイベロマイセス・ラクティス、トルラスポラ・デルブルッキー、キャンディダ・ユティリス、キャンディダ・ケフィア等があげられる。これらのイーストを単独で、又は複数種を組み合わせて使用することができる。さらに、これらのイーストと上述の乳酸菌を併用することも可能である。   Similarly, the yeast species is not particularly limited as long as it does not interfere with the object and effect of the present invention, and any yeast species can be used. Specifically, for example, Saccharomyces cerevisiae, Saccharomyces rosei, Saccharomyces cubatas, Saccharomyces schavarieri, Saccharomyces florentinas, Saccharomyces exeguiguas, Saccharomyces bailii, Kluyveromyces lactis, Torraspola debruky, Examples include Candida utilis and Candida kefir. These yeasts can be used alone or in combination. Furthermore, these yeasts and the above-mentioned lactic acid bacteria can be used in combination.

該小麦粉と水の構成割合も従来使用されてきた割合を用いることができる。具体的には、例えば、小麦粉に対して50〜150質量%の水を使用することができる。
また、乳酸菌及び/又はイーストの使用割合も従来使用されてきた割合を用いることができる。具体的には、例えば、該液状醗酵種中に1×10〜1010CFU/gの菌が存在するように添加することができる。
As the constituent ratio of the flour and water, a conventionally used ratio can be used. Specifically, for example, 50 to 150% by mass of water can be used with respect to the flour.
Moreover, the ratio used conventionally can also be used for the use ratio of lactic acid bacteria and / or yeast. Specifically, for example, it can be added so that 1 × 10 3 to 10 10 CFU / g of bacteria are present in the liquid fermentation species.

該液状醗酵種には糖類を含むことが望ましく、該糖類としては、添加する乳酸菌及び/又はイーストが資化できる糖であればよく、例えば、ブドウ糖、果糖、ショ糖、麦芽糖、乳糖、はちみつ、その他のものを用いることができる。そして、これらの糖類を1種又は複数種組み合わせて用いることもできる。該糖類の含有量は、該液状醗酵種を構成する小麦粉に対して1〜10質量%が望ましい。   The liquid fermented species preferably contains a saccharide, and the saccharide may be any sugar that can be assimilated by lactic acid bacteria and / or yeast to be added. For example, glucose, fructose, sucrose, maltose, lactose, honey, Others can be used. These saccharides can be used alone or in combination. As for content of this saccharide, 1-10 mass% is desirable with respect to the wheat flour which comprises this liquid fermentation seed | species.

該液状醗酵種を構成するその他の原料としては、従来醗酵種に使用されてきたものを用いることができる。具体的には、例えば、ライ麦等の小麦粉以外の穀粉、乳製品、油脂、塩、モルト、増粘剤等、本発明の目的・効果を妨げないものであれば、いずれのものも用いることができる。   As another raw material which comprises this liquid fermenting seed | species, what was conventionally used for the fermenting seed | species can be used. Specifically, for example, flours other than wheat flour such as rye, dairy products, fats and oils, salts, malts, thickeners and the like may be used as long as they do not interfere with the purpose and effect of the present invention. it can.

本発明では、該液状醗酵種として、種起しにより最初に調製して醗酵させた醗酵種(初種)を使用することができるし、又は該初種から出発し、この初種の少なくとも一部を最初の元種にして種継ぎを1回又は複数回若しくは無数に行って作成する醗酵種(以下継ぎ種という)を使用することができる。   In the present invention, the fermented seed (first seed) prepared and fermented first by seeding can be used as the liquid fermented seed, or at least one of the first seeds is started from the first seed. Fermentation seeds (hereinafter referred to as splicing seeds) that are created by performing seed splicing once or a plurality of times or innumerably using the part as the first original seed can be used.

該液状醗酵種の調製も初種及び種継ぎともに、従来の方法を用いることができる。具体的には、例えば、初種は前記原料を10〜30分間攪拌した後、27〜33℃で6〜48時間培養することができる。そして、該培養中には、定期的に間欠攪拌することが好ましい。以下の種継ぎをしないときには、攪拌後の元種を長時間、好ましくは40〜48時間培養したものを使用することが望ましい。   For the preparation of the liquid fermentation species, conventional methods can be used for both the initial species and the seed-joining. Specifically, for example, the first species can be cultured at 27 to 33 ° C. for 6 to 48 hours after stirring the raw material for 10 to 30 minutes. And it is preferable to intermittently stir periodically during the culture. When not splicing the following seeds, it is desirable to use the original seeds after stirring for a long time, preferably 40 to 48 hours.

種継ぎの方法としても、従来の方法を用いることができる。具体的には、例えば、前記液状醗酵種と同様の原料及び使用割合、即ち、小麦粉100質量%、水50〜150質量%、前記元種10〜50質量%、必要に応じて乳酸菌及び/又はイースト1×10〜1×1010CFU/g、糖類1〜10質量%とを混合・攪拌し、27〜33℃で6〜48時間培養するようにする。 A conventional method can also be used as a seeding method. Specifically, for example, the same raw materials and use ratio as the liquid fermentation species, that is, flour 100% by mass, water 50-150% by mass, the original species 10-50% by mass, and if necessary, lactic acid bacteria and / or Yeast 1 × 10 3 to 1 × 10 10 CFU / g and 1 to 10% by mass of saccharide are mixed and stirred, and cultured at 27 to 33 ° C. for 6 to 48 hours.

本発明の液状醗酵種は、初種でも、又は初種から出発して種継ぎをした継ぎ種でも、醗酵種として直ちにパンの製造に使用することができる程度まで培養して醗酵させておくことが望ましい。このためには、初種及び継ぎ種ともに、液状醗酵種の醗酵終了後のpHを4.0以下となるまで醗酵させることが望ましく、3.8以下となるまで醗酵させることがより望ましく、3.5〜3.7となるまで醗酵させることがより一層望ましい。   Even if the liquid fermented seed of the present invention is a first seed or a joint seed that has been spliced starting from the first seed, it should be cultured and fermented to the extent that it can be used immediately for the production of bread as a fermented seed. Is desirable. For this purpose, it is desirable to ferment both the initial and joint seeds until the pH of the liquid fermented seed after fermentation is 4.0 or less, more preferably 3.8 or less. It is even more desirable to ferment until it becomes 5-3.7.

これにより、醗酵終了後の液状醗酵種中の乳酸菌及び/又はイーストの数量が著しく増大することにより、後述する通り、該液状醗酵種に小麦粉を混合して作成した醗酵種生地を冷凍して解凍した後には、該醗酵種生地中には、著しく増量した乳酸菌及び/又はイーストの死滅菌体から多量のアミノ酸等の風味成分が放出されることになる。このように多量の風味成分を含有する醗酵種生地を使用して製造した焼成後のパンは、より一層良好な強い風味を有する。   As a result, the amount of lactic acid bacteria and / or yeast in the liquid fermented seeds after the fermentation is significantly increased, so that the fermented seed dough prepared by mixing flour with the liquid fermented seeds is frozen and thawed as described later. After that, a large amount of flavor components such as amino acids are released from the fermented seed dough from a significantly increased amount of lactic acid bacteria and / or dead sterilized yeast. Thus, the bread after baking manufactured using the fermented seed material | dough containing a lot of flavor components has a much more favorable strong flavor.

次に、このようにして調製し、培養して醗酵させた液状醗酵種に、少なくとも小麦粉を混合して非流動状の醗酵種生地を作成する。ここで液状醗酵種と小麦粉との割合は、非流動状の醗酵種生地を形成できる割合であれば良い。具体的には、例えば、液状醗酵種に対し30〜150質量%、好ましくは40〜50質量%の小麦粉を用いることができる。   Next, at least wheat flour is mixed with the liquid fermented seed prepared, cultured and fermented in this manner to produce a non-fluid fermented seed dough. Here, the ratio of the liquid fermented seed and the flour may be a ratio that can form a non-fluid fermented seed dough. Specifically, for example, 30 to 150% by mass, preferably 40 to 50% by mass of wheat flour can be used with respect to the liquid fermentation species.

該醗酵種生地を作成するにあたり、通常小麦粉以外の原料は必要でないが、小麦粉以外に、塩、糖類、油脂、脱脂粉乳等の乳由来原料、その他の通常の製パン用原料をそれぞれ適宜量添加することができる。   In preparing the fermented seed dough, raw materials other than wheat flour are usually not necessary, but in addition to flour, milk-derived raw materials such as salts, sugars, fats and oils, skim milk powder, and other ordinary bread-making raw materials are added in appropriate amounts. can do.

ここで、「非流動状」とは、例えば、パン生地のように、醗酵作用を加えない状態では、ある程度の保形性と可塑性を有する粘弾性のある状態をいう。より具体的には、少なくとも通常のパン生地の製造に用いる分割機や丸目機で分割や丸目ができる程度の状態である。   Here, the “non-fluid state” means a viscoelastic state having a certain degree of shape retention and plasticity in a state where no fermentation action is applied, such as bread dough. More specifically, it is in such a state that it can be divided or rounded by at least a dividing machine or a rounding machine used for producing ordinary bread dough.

前記液状醗酵種と小麦粉との混合は、小麦粉と液状醗酵種とが均一に練り込まれて、醗酵種生地が非流動状になるまで攪拌すれば良い。
更に、本発明においては、冷凍するにあたり、該醗酵種生地を極力醗酵させないで、該醗酵種生地の混合後速やかに冷凍するようにすることが可能であるし、望ましい。なぜなら、本発明は、後述する通り、乳酸菌及び/又はイーストが醗酵種生地中で醗酵して生成する物質によってパンに風味を付与することは従であり、むしろ主に醗酵種生地中で醗酵した乳酸菌及び/又はイーストの死滅した菌体から放出される風味成分によってパンに風味を付与するものであるから、ここでの醗酵は必要ではないのである。
The liquid fermented seed and flour may be mixed until the flour and liquid fermented seed are uniformly kneaded and the fermented seed dough becomes non-fluid.
Furthermore, in the present invention, it is possible and desirable to freeze the fermented seed dough as quickly as possible without freezing the fermented seed dough as much as possible. Because, as described later, the present invention is that the lactic acid bacteria and / or yeast fermented in the fermented seed dough to impart a flavor to the bread, but rather mainly fermented in the fermented seed dough. Fermentation here is not necessary because the flavor is imparted to the bread by the flavor components released from the dead cells of lactic acid bacteria and / or yeast.

ここで「速やかに」冷凍するとは、該混合後の醗酵種生地を、例えば、温湿度が管理された醗酵室を用いたりして、意図して醗酵させたり、熟成させたりしないで冷凍するという意味である。これに対し、混合後に醗酵種生地の捏上温度を測定したり、重量を測定したり、分割機に投入するために待機し、分割機がホッパー付きディバイダーである場合においてホッパーに投入してから実際に分割されるまでに待機し、及び/又は分割若しくは分割・丸め整形後ベルトコンベアー等の搬送装置で冷凍機まで搬送され、その他の該醗酵種生地の実際の工業的製造現場における製造工程上及び管理上不可避な時間の経過まで排除するという意味まではなく、このような時間の経過は許容される。具体的には、例えば、液状醗酵種と小麦粉との混合後、好ましくは50分以内に、より好ましくは40分以内に、さらに好ましくは30分以内に、さらにより好ましくは20分以内に、最も好ましくは10分以内に冷凍を開始することである。   Here, “quickly” freezing means that the fermented seed dough after mixing is frozen without intentionally fermenting or aging, for example, using a fermentation chamber in which the temperature and humidity are controlled. Meaning. On the other hand, after mixing, the temperature of the fermented seed dough is measured, the weight is measured, and it waits to be put into the dividing machine, and when the dividing machine is a divider with a hopper, In the manufacturing process at the actual industrial production site of the fermented seed dough, waiting until it is actually divided, and / or transported to the refrigerator by a conveyor such as a belt conveyor after division or division / rounding. In addition, there is no need to eliminate until the lapse of time that is unavoidable for management, and such passage of time is allowed. Specifically, for example, after mixing the liquid fermented seed and flour, preferably within 50 minutes, more preferably within 40 minutes, even more preferably within 30 minutes, even more preferably within 20 minutes, Preferably, freezing is started within 10 minutes.

これに対し、醗酵種生地の長時間醗酵は、液状醗酵種の醗酵と同様、不安定なものであるため、醗酵条件の厳格な管理が必要である。このための設備も必要となる。さもなければ、醗酵が醗酵種生地の分割工程の前でも後でも、該醗酵種生地の醗酵が不安定になり、焼成したパンの品質の安定性に影響するおそれがある。また、醗酵種生地の分割前に醗酵種生地を醗酵させ過ぎてしまうと、該醗酵種生地は半流動状となってしまい、機械的な分割・丸目がしにくくなる。更に、醗酵種生地の分割後に醗酵種生地を醗酵させ過ぎてしまうと、該醗酵種生地がイーストを含有している場合には、該醗酵種生地は膨張して流通や保管の際にスペースのロスになったり、また、膨張した該醗酵種生地の表皮は弱くなり、冷凍保管や配送中に表皮が破れたり、崩れたりして旨味成分を逃がす等の弊害が生じる。   On the other hand, the long-time fermentation of the fermented seed dough is unstable, as is the case with the liquid fermented seed, and thus requires strict management of the fermentation conditions. Equipment for this is also required. Otherwise, the fermentation of the fermented seed dough becomes unstable both before and after the step of dividing the fermented seed dough, which may affect the stability of the quality of the baked bread. In addition, if the fermented seed dough is fermented too much before the fermented seed dough is divided, the fermented seed dough becomes semi-fluid and mechanical division / rounding is difficult. Furthermore, if the fermented seed dough is excessively fermented after the fermentation seed dough is divided, if the fermented seed dough contains yeast, the fermented seed dough expands and the space of the fermented seed dough is increased during distribution and storage. The skin of the fermented dough that has been lost or swelled is weakened, and the skin is broken or broken during frozen storage or delivery, resulting in problems such as escaping umami components.

また、該醗酵種生地を冷凍するにあたり、混合後の該醗酵種生地を小さく、例えば、具体的には、50〜500gに分割し、丸め整形をすることが望ましい。こうすることにより、冷凍効率、取り扱いや保管の容易性、更には、製パン時の作業性が向上する。なお、上述の丸め整形とは、丸めて球状に整形することだけでなく、なまこ状、板状、棒状、その他本発明の目的・効果を妨げるものでなければ、いずれの形状に整形することをも含む意味である。   Moreover, in freezing the fermented seed dough, it is desirable that the fermented seed dough after mixing is made small, for example, specifically divided into 50 to 500 g and rounded. By doing so, refrigeration efficiency, ease of handling and storage, and workability during breadmaking are improved. In addition, the above rounding and shaping means not only rounding and shaping into a spherical shape, but also shaping into any shape as long as it does not hinder the purpose and effect of the present invention, such as a sea cucumber shape, a plate shape, a rod shape. It also includes the meaning.

そして、該混合後の醗酵種生地を分割するにあたり、該醗酵種生地を混合後直ちに分割するようにする。ここでも「直ちに」分割するとは、該混合後の醗酵種生地を、例えば、温湿度が管理された醗酵室を用いたりして、意図して醗酵させたり、熟成させたりしないで、可及的速やかに分割するという意味である。これに対し、上述した通り、捏上温度、重量の測定、分割の待機、その他の次工程までの不可避的な時間の経過まで排除するという意味まではない。該経過時間は、好ましくは30分程度以下であり、より好ましくは20分以下であり、さらに好ましくは10分以下である。   And in dividing the fermented seed dough after mixing, the fermented seed dough is divided immediately after mixing. Here, “immediately” means that the fermented seed dough after mixing is made as much as possible without intentionally fermenting or aging, for example, using a fermentation room in which temperature and humidity are controlled. It means to divide quickly. On the other hand, as described above, there is no point in eliminating the unavoidable elapse of time until the next step, as described above, on the soot temperature, weight measurement, waiting for division. The elapsed time is preferably about 30 minutes or less, more preferably 20 minutes or less, and even more preferably 10 minutes or less.

なお、該混合後の醗酵種生地を直ちに分割する理由は、上述した通りである。また、さらには、該醗酵種生地を分割前に醗酵させると、該醗酵時間が長ければ長いほど、該醗酵種生地中に当初から含有されている乳酸菌以外の菌種の乳酸菌や酵母が増殖し、この結果、該醗酵種生地が当初の目的としているパンの風味や製パン性を得ることが妨げられるおそれがある。   The reason for immediately dividing the fermented seed dough after mixing is as described above. Furthermore, when the fermented seed dough is fermented before division, the longer the fermentation time, the more lactic acid bacteria and yeasts of the species other than the lactic acid bacteria contained in the fermented seed dough from the beginning grow. As a result, there is a possibility that the fermentation seed dough may be prevented from obtaining the bread flavor and bread-making properties that are originally intended.

このようにして製造した冷凍醗酵種生地は、解凍することにより直ちにパン生地の混捏工程で添加することができる。
冷凍した醗酵種生地は、使用時まで、その製造場所で冷凍保管されたり、使用場所まで冷凍配送されたり、または第三者の冷凍倉庫や物流施設に冷凍保管されたりする。
The frozen fermented seed dough produced in this way can be immediately added in the kneading process of bread dough by thawing.
The frozen fermented seed dough is stored frozen at its production site until it is used, delivered frozen to the site of use, or stored frozen in a third-party freezing warehouse or distribution facility.

また、本発明は、上述した冷凍醗酵種生地を解凍してからパン生地の混捏工程で添加するパン生地であり、また該パン生地を焼成するパンである。
該冷凍醗酵種生地の解凍方法は、常温解凍、高温短時間解凍、低温長時間解凍、その他いずれの方法も採用可能である。解凍の程度は、パン生地の混捏工程でその他のパン生地原料と均一に混合できる程度であれば良いが、該冷凍醗酵種生地全体が完全に解凍されるまで解凍するのが望ましい。
In addition, the present invention is a bread dough that is added in the kneading process of bread dough after thawing the above-mentioned frozen fermented seed dough, and is a bread for baking the bread dough.
As a method for thawing the frozen fermented seed dough, any method such as room temperature thawing, high temperature short time thawing, low temperature long time thawing, or the like can be employed. The degree of thawing may be such that it can be uniformly mixed with other bread dough ingredients in the dough mixing process, but it is desirable to thaw until the entire frozen fermented seed dough is completely thawed.

前記醗酵種生地は、含有する乳酸菌及び/又はイースト菌体が液状醗酵種の培養中に多量の水分を吸収して大きく膨潤していることから、これを冷凍することにより、該菌体の容積が拡大して破裂し崩壊した状態で冷凍される。これにより、乳酸菌及び/又はイーストは全て又はほとんどが死滅してしまい活性を示さないようになる。そして、該冷凍醗酵種生地を解凍したときに、崩壊した菌体からアミノ酸、酵母エキス等の風味成分が解凍後の醗酵種生地中に放出される。このため、該醗酵種生地を使用して焼成したパンは、従来の醗酵種を添加して焼成したパンよりも良好で強い風味を有するようになる。   Since the lactic acid bacteria and / or yeast cells contained in the fermented seed dough absorb a large amount of water during the culture of the liquid fermented seeds and are greatly swollen, the volume of the cells is increased by freezing it. It expands, bursts and freezes in a collapsed state. As a result, all or most of the lactic acid bacteria and / or yeast are killed and no activity is exhibited. When the frozen fermented seed dough is thawed, flavor components such as amino acids and yeast extract are released from the disintegrated cells into the thawed fermented seed dough. For this reason, the bread baked using this fermented seed dough comes to have a better and stronger flavor than bread baked by adding conventional fermented seeds.

このためには、前記解凍後の醗酵種生地中におけるアミノ酸の含有量が500ppm以上であることが望ましく、600ppm以上であることがより望ましく、700ppm以上であることがより一層望ましい。   For this purpose, the content of amino acids in the fermented seed dough after thawing is desirably 500 ppm or more, more desirably 600 ppm or more, and even more desirably 700 ppm or more.

前記醗酵種生地は、パン生地を構成する小麦粉に対し1〜40質量%添加することが望ましく、3〜30質量%添加することがより望ましく、5〜20質量%添加することがより一層望ましい。   The fermented seed dough is preferably added in an amount of 1 to 40% by mass, more preferably 3 to 30% by mass, and still more preferably 5 to 20% by mass with respect to the wheat flour constituting the bread dough.

本発明の冷凍醗酵種生地を用いるパン生地の製法としては、中種法、ストレート法、ノータイム法、短時間製法等、特に制限なく各種製法を採用することができる。
また、本発明の冷凍醗酵種生地を解凍した醗酵種生地は、パン生地の混捏工程のどの段階で添加してもよい。例えば、中種法によりパン生地を作成する場合には、該醗酵種生地を中種の混捏工程と本捏工程のどちらか一方又は両方で添加することができる。
As a method for producing bread dough using the frozen fermented seed dough of the present invention, various production methods such as a medium seed method, a straight method, a no-time method, a short-time production method and the like can be adopted without particular limitation.
Moreover, the fermented seed dough obtained by thawing the frozen fermented seed dough of the present invention may be added at any stage of the kneading process of bread dough. For example, when producing bread dough by the middle seed method, the fermented seed dough can be added in either one or both of the middle kneading process and the main koji process.

そして、本発明は、食パン、フランスパン、菓子パン、バンズ、コーヒーケーキ、蒸しパン、ピザ、ドーナツ、スイートロール、ロール、ペストリー、デニッシュペストリー等の各種パンの製造にも用いることができる。好適には、食パンやフランスパン等のリーンな配合のパンの製造に用いられる。   And this invention can be used also for manufacture of various breads, such as bread, French bread, sweet bread, buns, coffee cake, steamed bread, pizza, donut, sweet roll, roll, pastry, Danish pastry. Preferably, it is used for the production of lean blends such as bread and French bread.

本発明は、液状醗酵種に小麦粉を混合して非流動状の醗酵種生地を作成し、冷凍することによって製造した冷凍醗酵種生地に関する。該冷凍醗酵種生地は、従来の液状醗酵種に比べ、配送、保管等の流通時における管理および取り扱い、また、製造ラインにおけるミキサー担当者の作業性が容易である。   The present invention relates to a frozen fermented seed dough produced by mixing non-fluid fermented seed dough by mixing flour with liquid fermented seed and freezing. Compared with the conventional liquid fermented seeds, the frozen fermented seed dough is easier to manage and handle at the time of distribution such as delivery and storage, and workability of the person in charge of the mixer in the production line.

また、本発明においては、その醗酵種生地内に含有される乳酸菌及び/又はイースト菌体が、液状醗酵種の培養中に多量の水分を吸収して大きく膨潤していることから、これを冷凍すると、該菌体の容積が拡大して破裂し、該菌体が崩壊した状態で冷凍される。これにより、乳酸菌及び/又はイーストは全て又はほとんどが死滅してしまい活性を示さないようになる。そして、該冷凍醗酵種生地を解凍したときに、崩壊した菌体からアミノ酸、酵母エキス等の風味成分が解凍後の醗酵種生地中に放出される。このため、該醗酵種生地を使用して焼成したパンは、従来の醗酵種を添加して焼成したパンよりも良好で強い風味を有するものとなる。   In the present invention, the lactic acid bacteria and / or yeast cells contained in the fermented seed dough absorb a large amount of water during the cultivation of the liquid fermented seeds, and are greatly swollen. The cell body expands and bursts, and the cell body is frozen in a collapsed state. As a result, all or most of the lactic acid bacteria and / or yeast are killed and no activity is exhibited. When the frozen fermented seed dough is thawed, flavor components such as amino acids and yeast extract are released from the disintegrated cells into the thawed fermented seed dough. For this reason, bread baked using the fermented seed dough has a stronger and stronger flavor than bread baked by adding conventional fermented seeds.

一般に、パン生地に従来の液状醗酵種を添加して製造されたパン生地は、液状醗酵種を添加しないで製造されたパン生地と比較して、伸展性が良くなる傾向にある。これは、液状醗酵種中の乳酸又はイーストがグルテニンの弾性を改変させるためであると推測される。本発明の冷凍醗酵種生地を用いて製造したパン生地も、従来の液状醗酵種を添加して製造したパン生地同様の伸展性の改善されたものとなる。   In general, bread dough produced by adding conventional liquid fermented seeds to bread dough tends to improve extensibility compared to bread dough produced without adding liquid fermented seeds. This is presumed to be because lactic acid or yeast in the liquid fermentation species modifies the elasticity of glutenin. The bread dough manufactured using the frozen fermented seed dough of the present invention also has improved extensibility similar to bread dough manufactured by adding a conventional liquid fermented seed.

また、従来の液状醗酵種をパン生地に添加して製造されたパンは、液状醗酵種を添加しないで製造されたパンと比較して、老化が遅くなる傾向にある。これは、液状醗酵種中の乳酸又はイーストが有する老化抑制・保湿性向上効果によるものであると推測される。本発明の冷凍醗酵種生地を用いて製造したパン生地を焼成して得たパンは、従来の液状醗酵種をパン生地に添加して製造されたパンよりも、老化遅延効果が大きいものである。   Moreover, the bread manufactured by adding the conventional liquid fermented seeds to the bread dough tends to be delayed in aging compared to the bread manufactured without adding the liquid fermented seeds. This is presumed to be due to the effect of inhibiting aging and improving the moisturizing property of lactic acid or yeast in the liquid fermentation species. Bread obtained by baking bread dough produced using the frozen fermented seed dough of the present invention has a greater aging delay effect than bread produced by adding conventional liquid fermentation seeds to bread dough.

[実施例1−1]
冷凍醗酵種生地の調製
表1に示した原料を20分間攪拌した後、27℃で45時間培養した。培養中、定期的に間欠攪拌し、液状醗酵種を得た。該液状醗酵種のpHは3.7であり、酸味を有するものであった。
[Example 1-1]
Preparation of frozen fermented seed dough The raw materials shown in Table 1 were stirred for 20 minutes and then cultured at 27 ° C. for 45 hours. During the culture, intermittent stirring was periodically performed to obtain a liquid fermented seed. The pH of the liquid fermentation species was 3.7 and had a sour taste.

該液状醗酵種に対し、小麦粉(強力粉)45質量%を添加し、ミキサーで80rpmにて2分間ミキシングを行い、非流動状の醗酵種生地を得た。
該醗酵種生地を速やかに100gに分割して丸めて、該分割後の醗酵種生地を−40℃の雰囲気下に24分間置き、冷凍醗酵種生地とし、該冷凍醗酵種生地を包装した。このようにして得られた冷凍醗酵種生地は、冷凍されているため、長期保管しても過醗酵、変敗、活性の喪失等の心配がなく、長期保管が可能であるとともに、取り扱いが容易であった。しかも、該冷凍醗酵種生地は、従来の冷凍パン生地と同様の冷凍配送・保管設備が利用できる利点を有する。そして、使用する際には、解凍して使用する。
45 mass% of wheat flour (strong flour) was added to the liquid fermented seed, and the mixture was mixed with a mixer at 80 rpm for 2 minutes to obtain a non-fluid fermented seed dough.
The fermented seed dough was quickly divided into 100 g and rounded, and the fermented seed dough after the division was placed in an atmosphere of −40 ° C. for 24 minutes to form a frozen fermented seed dough, and the frozen fermented seed dough was packaged. The frozen fermented seed dough obtained in this way is frozen, so there is no concern about over-fermentation, deterioration, loss of activity, etc. even after long-term storage, and long-term storage is possible and handling is easy. Met. Moreover, the frozen fermented seed dough has the advantage that the same frozen delivery / storage facilities as those of conventional frozen bread dough can be used. And when using it, it defrosts and uses.

Figure 2006204286
Figure 2006204286

[実施例1−2]
表2に示す配合からなる原料を混合し、該混合物を27℃で27時間醗酵させて液状醗酵種(初種)の種起こしを行った。
[Example 1-2]
The raw material which consists of a mixing | blending shown in Table 2 was mixed, and this mixture was fermented at 27 degreeC for 27 hours, and the liquid fermentation seed | species (first seed | species) was seeded.

Figure 2006204286
Figure 2006204286

該液状醗酵種の一部を元種として、以下の配合からなる原料を混合し、該混合物を27℃で24時間醗酵させて液状醗酵種(継ぎ種)を調製した。   A part of the liquid fermented seed was used as an original seed, raw materials having the following composition were mixed, and the mixture was fermented at 27 ° C. for 24 hours to prepare a liquid fermented seed (joint seed).

Figure 2006204286
Figure 2006204286

該継ぎ種の一部を元種として、同一の配合からなる原料を混合し、該混合物を27℃で24時間、定期的に攪拌しながら、醗酵させて液状醗酵種(継ぎ種)を調製した。
該継ぎ種の一部を元種として、同一の配合からなる原料を混合し、該混合物を27℃で12時間、定期的に攪拌しながら、醗酵させて液状醗酵種(継ぎ種)を調製し、10℃で12時間保存した。
A raw material having the same composition was mixed with a part of the joint seed as the original seed, and the mixture was fermented at 27 ° C. for 24 hours with regular stirring to prepare a liquid fermented seed (joint seed). .
A part of the joint seed is used as an original seed, raw materials having the same composition are mixed, and the mixture is fermented at 27 ° C. with regular stirring for 12 hours to prepare a liquid fermented seed (joint seed). Stored at 10 ° C. for 12 hours.

該保存後の継ぎ種の一部を元種として、以下の配合からなる原料を混合し、該混合物を27℃で8時間醗酵させて液状醗酵種(継ぎ種)を調製して、10℃で保存する。これを何回も繰り返す。   Using the part of the spliced seed after storage as the original seed, the raw materials consisting of the following blends are mixed, and the mixture is fermented at 27 ° C. for 8 hours to prepare a liquid fermented seed (joint seed) at 10 ° C. save. Repeat this many times.

Figure 2006204286
Figure 2006204286

このように繰り返し種継ぎをして得た液状醗酵種100質量%に対し、小麦粉(強力粉40質量%を添加し、ミキサーで80rpmにて4分間ミキシングを行い、非流動状の醗酵種生地を得た。   In this way, 100% by mass of the liquid fermented seed obtained by repeated seeding is added flour (40% by weight of strong flour and mixed for 4 minutes at 80 rpm with a mixer to obtain a non-fluid fermented seed dough. It was.

該醗酵種生地を速やかに100gに分割し、該分割後の醗酵種生地を−40℃の雰囲気下に24分間置き、冷凍醗酵種生地とした。
このようにして得られた冷凍醗酵種生地は、冷凍されているため、長期保管においても過醗酵、変敗、活性の喪失等の心配がなく、よって長期保管が可能となるとともに、取り扱いが容易であった。しかも、該冷凍醗酵種生地は、従来の冷凍パン生地と同様の冷凍配送・保管設備が利用できる利点を有する。そして、使用するときには、解凍して使用する。
[実施例1−3]
表5に示す配合からなる原料を混合し、該混合物を27℃で27時間醗酵させて液状醗酵種(初種)の種起こしを行った。
The fermented seed dough was quickly divided into 100 g, and the fermented seed dough after the division was placed in an atmosphere of −40 ° C. for 24 minutes to obtain a frozen fermented seed dough.
Since the frozen fermented seed dough thus obtained is frozen, there is no concern about over-fermentation, deterioration, loss of activity, etc. even during long-term storage, thus enabling long-term storage and easy handling. Met. Moreover, the frozen fermented seed dough has the advantage that the same frozen delivery / storage facilities as those of conventional frozen bread dough can be used. And when using, it thaws and uses.
[Example 1-3]
The raw material which consists of a mixing | blending shown in Table 5 was mixed, and this mixture was fermented at 27 degreeC for 27 hours, and the liquid fermentation seed | species (first seed | species) was seeded.

Figure 2006204286
Figure 2006204286

該液状醗酵種の一部を元種として、以下の配合からなる原料を混合し、該混合物を27℃で24時間醗酵させて液状醗酵種(継ぎ種)を調製した。   A part of the liquid fermented seed was used as an original seed, raw materials having the following composition were mixed, and the mixture was fermented at 27 ° C. for 24 hours to prepare a liquid fermented seed (joint seed).

Figure 2006204286
Figure 2006204286

該継ぎ種の一部を元種として、同一の配合からなる原料を混合し、該混合物を27℃で24時間、定期的に攪拌しながら、醗酵させて液状醗酵種(継ぎ種)を調製した。
該継ぎ種の一部を元種として、同一の配合からなる原料を混合し、該混合物を27℃で12時間、定期的に攪拌しながら、醗酵させて液状醗酵種(継ぎ種)を調製し、10℃で12時間保存した。
A raw material having the same composition was mixed with a part of the joint seed as the original seed, and the mixture was fermented at 27 ° C. for 24 hours with regular stirring to prepare a liquid fermented seed (joint seed). .
A part of the joint seed is used as an original seed, raw materials having the same composition are mixed, and the mixture is fermented at 27 ° C. with regular stirring for 12 hours to prepare a liquid fermented seed (joint seed). Stored at 10 ° C. for 12 hours.

該保存後の継ぎ種の一部を元種として、表7の配合からなる原料を混合し、該混合物を27℃で8時間醗酵させて液状醗酵種(継ぎ種)を調製して、10℃で保存する。これを何回も繰り返す。   Using the part of the seeds after storage as the original seed, the raw materials having the composition shown in Table 7 are mixed, and the mixture is fermented at 27 ° C. for 8 hours to prepare a liquid fermented seed (joint seed). Save with. Repeat this many times.

Figure 2006204286
Figure 2006204286

このように繰り返し種継ぎをした継ぎ種を元種として、以下の配合からなる原料を混合し、該混合物を27℃で8時間醗酵させて液状醗酵種(継ぎ種)を調製した。このような種継ぎを2回繰り返した。該調製後の液状醗酵種のpHは3.8だった。該液状醗酵種を10℃にて保管した。   The raw material having the following composition was mixed using the joint seed repeatedly seeded in this way, and the mixture was fermented at 27 ° C. for 8 hours to prepare a liquid fermented seed (joint seed). Such seeding was repeated twice. The pH of the liquid fermented seed after the preparation was 3.8. The liquid fermentation species was stored at 10 ° C.

Figure 2006204286
Figure 2006204286

このように2回の種継ぎをして得た液状醗酵種100質量%に対し、小麦粉(強力粉)45質量%を添加し、ミキサーで80rpmにて4分間ミキシングを行い、非流動状の醗酵種生地を得た。   Thus, 45 mass% of flour (strong flour) is added to 100 mass% of the liquid fermented seed obtained by performing the seeding twice, and the mixture is mixed with a mixer at 80 rpm for 4 minutes, and the non-fluid fermented seed I got the dough.

該醗酵種生地を速やかに100gに分割して丸め、該分割後の醗酵種生地を−40℃の雰囲気下に24分間置き、冷凍醗酵種生地とし、該冷凍醗酵種生地を包装した。このようにして得られた冷凍醗酵種生地は、冷凍されているため、長期保管においても過醗酵、変敗、活性の喪失等の心配がなく、よって長期保管が可能となるとともに、取り扱いが容易であった。しかも、該冷凍醗酵種生地は、従来の冷凍パン生地と同様の冷凍配送・保管設備が利用できる利点を有する。そして、使用するときには、解凍して使用する。
[実施例1−4]
表9に示した原料を20分間攪拌した後、27℃で45時間培養した。培養中、定期的に間欠攪拌し、液状醗酵種を得た。該液状醗酵種のpHは3.7であり、酸味を有するものであった。
The fermented seed dough was quickly divided into 100 g and rounded, and the fermented seed dough after the division was placed in an atmosphere of −40 ° C. for 24 minutes to form a frozen fermented seed dough, and the frozen fermented seed dough was packaged. Since the frozen fermented seed dough thus obtained is frozen, there is no concern about over-fermentation, deterioration, loss of activity, etc. even during long-term storage, thus enabling long-term storage and easy handling. Met. Moreover, the frozen fermented seed dough has the advantage that the same frozen delivery / storage facilities as those of conventional frozen bread dough can be used. And when using, it thaws and uses.
[Example 1-4]
The raw materials shown in Table 9 were stirred for 20 minutes and then cultured at 27 ° C. for 45 hours. During the culture, intermittent stirring was periodically performed to obtain a liquid fermented seed. The pH of the liquid fermentation species was 3.7 and had a sour taste.

該液状醗酵種に対し、小麦粉(強力粉)40質量%を添加し、ミキサーで80rpmにて2分間ミキシングを行い、非流動状の醗酵種生地を得た。
該醗酵種生地を速やかに100gに分割して丸めて、該分割後の醗酵種生地を−40℃の雰囲気下に24分間置き、冷凍醗酵種生地とし、該冷凍醗酵種生地を包装した。このようにして得られた冷凍醗酵種生地は、冷凍されているため、長期保管しても過醗酵、変敗、活性の喪失等の心配がなく、長期保管が可能であるとともに、取り扱いが容易であった。しかも、該冷凍醗酵種生地は、従来の冷凍パン生地と同様の冷凍配送・保管設備が利用できる利点を有する。そして、使用する際には、解凍して使用する。
40 mass% of wheat flour (strong flour) was added to the liquid fermented seed, and the mixture was mixed with a mixer at 80 rpm for 2 minutes to obtain a non-fluid fermented seed dough.
The fermented seed dough was quickly divided into 100 g and rounded, and the fermented seed dough after the division was placed in an atmosphere of −40 ° C. for 24 minutes to form a frozen fermented seed dough, and the frozen fermented seed dough was packaged. The frozen fermented seed dough obtained in this way is frozen, so there is no concern about over-fermentation, deterioration, loss of activity, etc. even after long-term storage, and long-term storage is possible and handling is easy. Met. Moreover, the frozen fermented seed dough has the advantage that the same frozen delivery / storage facilities as those of conventional frozen bread dough can be used. And when using it, it defrosts and uses.

Figure 2006204286
Figure 2006204286

[実施例2]
前記実施例1−2において製造した冷凍醗酵種生地を27℃で約2時間解凍し、該解凍された醗酵種生地を用いて、表10および表11に示す原料配合・工程により全粒粉入りフランスパンを作成した。
[Example 2]
The frozen fermented seed dough produced in Example 1-2 was thawed at 27 ° C. for about 2 hours, and the thawed fermented seed dough was used to prepare French bread containing whole grains according to the raw material blending / steps shown in Table 10 and Table 11. It was created.

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

成形後の実施例2のパン生地は、伸展性が良く伸びやすい良好な生地物性であった。そして、実施例2のパンは、形状が安定していて良好で、焼色は明るい黄金色であった。そして、内相は伸びが有り、膜が薄く良好であった。また、香りは全粒粉由来の穀物臭が僅かに残存しているものの、香ばしく、食感は軽く良好であった。しかも、老化の抑制されたものであった。
[実施例3]
本発明の実施例3−1及び3−2として、前記実施例1−3の冷凍醗酵種生地を27℃で約2時間解凍し、該解凍された醗酵種生地を用いて以下の原料配合・工程によりフランスパンを作成した。
The bread dough of Example 2 after molding had good dough physical properties with good extensibility and easy elongation. And the bread of Example 2 was stable and good in shape, and the baking color was a bright golden color. The inner phase was elongated and the film was thin and good. In addition, the scent was fragrant and the texture was light and good, although the grain odor derived from the whole grain flour remained slightly. Moreover, aging was suppressed.
[Example 3]
As Examples 3-1 and 3-2 of the present invention, the frozen fermented seed dough of Example 1-3 was thawed at 27 ° C. for about 2 hours, and using the thawed fermented seed dough, the following raw material composition / French bread was made by the process.

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

成形後の実施例3−1及び3−2のパン生地は、伸展性が良く伸びやすい良好な生地物性であった。そして、実施例3−1及び3−2のパンは、形状が良好で、焼色は明るく、内相は伸びが有り、膜が薄く良好であった。また、両者とも甘い香りがして、良好な食感であった。実施例3−1のパンは、ややコクが感じられる味であった。実施例3−2のパンは、実施例3−1のパンよりもコクが強く感じられ、更に旨味の有る味であった。実施例3−1及び3−2のパンともに老化の抑制されたものであった。
[実施例4]
実施例4として、前記実施例1−4の冷凍醗酵種生地を27℃で約2時間解凍し、該解凍された醗酵種生地を用いて、表14および表15の原料配合・工程によりフランスパンを作成した。
The bread dough of Examples 3-1 and 3-2 after molding had good dough physical properties with good extensibility and easy elongation. The breads of Examples 3-1 and 3-2 were good in shape, bright in color, bright in the inner phase, and thin and good in film. Moreover, both had a sweet scent and a good texture. The bread of Example 3-1 had a taste that was somewhat rich. The bread of Example 3-2 felt richer than the bread of Example 3-1, and had a more delicious taste. Aging was suppressed in both the breads of Examples 3-1 and 3-2.
[Example 4]
As Example 4, the frozen fermented seed dough of Example 1-4 was thawed at 27 ° C. for about 2 hours, and the thawed fermented seed dough was used to prepare French bread according to the raw material blending / process of Tables 14 and 15. It was created.

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

成形後の実施例4のパン生地は、伸展性が良く伸びやすい良好な生地物性であった。そして、実施例4のパンは、形状が良好で、焼色は明るく、内相は伸びが有り、膜が薄く良好であった。また、実施例4のパンは、甘い香りがして、良好な食感であった。しかも、コクが強く感じられ、更に旨味の有る味であった。実施例4のパンは、老化の抑制されたものであった。
[実施例5−1]
前記実施例1−2における醗酵種生地30質量%を、前記実施例1−3の冷凍醗酵種生地を5℃で約20時間解凍して得られた醗酵種生地10質量%(実施例5−1)、同じく前記実施例1−3の冷凍醗酵種生地を5℃で約20時間解凍して得られた醗酵種生地20質量%(実施例5−2)に代えて、それ以外は実施例2と同様の原料配合・工程により全粒粉入りフランスパンを作成した。
The bread dough of Example 4 after molding had good dough physical properties with good extensibility and easy elongation. The bread of Example 4 was good in shape, bright in color, bright in the inner phase, thin and good in film. Moreover, the bread of Example 4 had a sweet scent and a good texture. Moreover, the richness was felt strongly and the taste was even more delicious. In the bread of Example 4, aging was suppressed.
[Example 5-1]
30% by mass of the fermented seed dough in Example 1-2 and 10% by mass of the fermented seed dough obtained by thawing the frozen fermented seed dough of Example 1-3 at 5 ° C. for about 20 hours (Example 5- 1) In place of 20 mass% (Example 5-2) of fermented seed dough obtained by thawing the frozen fermented seed dough of Example 1-3 at 5 ° C. for about 20 hours. French bread with whole grains was prepared by the same raw material composition and process as in No. 2.

実施例5−1及び5−2のパンは、焼色が明るい黄金色で良好であった。そして、全粒粉由来の穀物臭がマスキングされており香ばしい良好な風味を有していた。食べてみるとやや甘みが感じられ、コクが有るとともに、軽くてサクい、しかもソフトな食感であった。
[実施例6]
本発明の実施例6として、前記実施例1−1で作成した冷凍醗酵種生地を27℃で約2時間解凍して得た醗酵種生地を使用して、以下の配合及び工程によりフランスパンを作成した。
The breads of Examples 5-1 and 5-2 were good in bright golden color. And the grain odor derived from the whole grain was masked and had a fragrant good flavor. When I ate it, it was a little sweet, rich and light, crunchy and soft.
[Example 6]
As Example 6 of the present invention, using the fermented seed dough obtained by thawing the frozen fermented seed dough prepared in Example 1-1 at 27 ° C. for about 2 hours, French bread was prepared by the following composition and process. Created.

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

このようにして焼成したフランスパンは、焼色が明るく良好であり、香りは香ばしく、また味・風味もフランスパン独特の塩味が強く感じられ、旨味があった。
[実施例7]
本発明の実施例7として、前記実施例6と同様に前記実施例1−1で作成した冷凍醗酵種生地を27℃で約2時間解凍して得た醗酵種生地を使用して、今度は以下の配合及び工程により食パンを作成した。
The French bread baked in this way had a bright and good baked color, a fragrant fragrance, and a strong salty taste unique to the French bread.
[Example 7]
As Example 7 of the present invention, using the fermented seed dough obtained by thawing the frozen fermented seed dough prepared in Example 1-1 in the same manner as in Example 6 at 27 ° C. for about 2 hours, this time A bread was prepared by the following composition and process.

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

Figure 2006204286
Figure 2006204286

このようにして焼成した食パンは、形状はケーブインが少なくて良好であり、焼色も明るく良好である。また、食感は比較的軽くて良好であり、香りは中種法で作成した食パンに特徴的なツンと鼻をつくイースト醗酵臭(アルコール臭)がなく、香ばしい小麦粉醗酵臭があり、味・風味もやや酸味を有する旨味を感じさせるものであった。   The bread baked in this way has a good shape with little cave-in, and the baking color is bright and good. In addition, the texture is relatively light and good, and the fragrance is free from the yeast fermentation smell (alcoholic odor) that is characteristic of bread made by the middle-class method, and has a fragrant flour fermentation odor. The flavor also has a slightly sour taste.

実験例Experimental example

実験例1Experimental example 1

実施例1−4で得た冷凍醗酵種生地を27℃で約2時間解凍した醗酵種生地と、実施例1−4で得た液状醗酵種で冷凍前のもの(比較例1)のアミノ酸分析を日本電子社製アミノ酸自動分析器JEOL−500/Vを用いて行い、各種アミノ酸量を比較・検討した。分析結果を図1に示す(なお、醗酵種生地は、液状醗酵種に小麦粉を添加したものであるため、分析項目の成分値は実際の分析値から該小麦粉がもたらす成分量を差し引いた値に換算した)。   Amino acid analysis of the fermented seed dough obtained by thawing the frozen fermented seed dough obtained in Example 1-4 at 27 ° C. for about 2 hours and the liquid fermented seed obtained in Example 1-4 before freezing (Comparative Example 1). Was performed using an amino acid automatic analyzer JEOL-500 / V manufactured by JEOL Ltd., and various amino acid amounts were compared and examined. The analysis result is shown in FIG. 1 (in addition, since the fermented seed dough is obtained by adding flour to the liquid fermented seed, the component value of the analysis item is the value obtained by subtracting the amount of component that the flour brings from the actual analyzed value. Converted).

実施例1−4で得た冷凍醗酵種生地を解凍して得た醗酵種生地は、比較例1の液状醗酵種と比較すると、分析した全アミノ酸項目で値が増加していた。特に、酸味及び旨味の呈味を有するアスパルギン酸(Asp)及びグルタミン酸(Glu)、甘味及び旨味の呈味を有するセリン(Ser)及びリジン(Lys)並びに甘味の呈味を有するスレオニン(Thr)の増加率が大きかった。各項目の分析値の合計は、比較例1の液状醗酵種が461ppmであったのに対し、実施例1−4で得た冷凍醗酵種生地を解凍して得た醗酵種生地は714ppmと、253ppm増加していた。これは、液状醗酵種中の乳酸菌体が培養中に水分を吸収して大きく膨潤しているところ、これを冷凍することにより菌体の容積が拡大して破裂し崩壊した状態となって冷凍され、次いで解凍すると、崩壊した菌体からアミノ酸が醗酵種生地中に放出されたためであると推測された。アミノ酸は、食品の呈味成分として知られており、従って、アミノ酸、特に、旨味及び甘味を有するアミノ酸量が増加した本発明の醗酵種生地は、パンの旨味・風味の向上・増強効果を有するものといえる。   The fermented seed dough obtained by thawing the frozen fermented seed dough obtained in Example 1-4 had increased values in all amino acid items analyzed when compared with the liquid fermented seed of Comparative Example 1. In particular, aspartic acid (Asp) and glutamic acid (Glu) having sour and umami tastes, serine (Ser) and lysine (Lys) having sweet and umami tastes, and threonine (Thr) having sweet tastes. The rate of increase was large. The total analysis value of each item was 461 ppm for the liquid fermentation species of Comparative Example 1, whereas the fermentation species dough obtained by thawing the frozen fermentation seed fabric obtained in Example 1-4 was 714 ppm, It was increased by 253 ppm. This is because the lactic acid bacteria in the liquid fermented species absorbs water during the culture and swells greatly, and by freezing it, the volume of the bacteria expands and ruptures and collapses. Then, when thawed, it was speculated that amino acids were released from the disintegrated cells into the fermented seed dough. Amino acids are known as food flavor components, and therefore the fermented seed dough of the present invention having an increased amount of amino acids, particularly amino acids having umami and sweetness, has an effect of improving and enhancing bread umami and flavor. It can be said that.

実験例2Experimental example 2

本発明として前記実施例3−2のパンと、比較例として該実施例3−2において醗酵種生地を添加しないようにして、それ以外は該実施例3−2と同様の原料配合・工程によって得たフランスパン(比較例3)とを用いて官能評価試験を行った。試験方法は、官能評価試験の訓練を受けた男女32名のパネラーによる7段階評価で、平均値を求めるとともに、t−検定により、総合嗜好、味嗜好、味識別及び香識別を比較した。試験結果を表22に示す。   According to the present invention, the bread of Example 3-2 and, as a comparative example, the fermentation seed dough was not added in Example 3-2, and the other ingredients were mixed and processed in the same manner as in Example 3-2. A sensory evaluation test was performed using the obtained French bread (Comparative Example 3). The test method was a seven-step evaluation by panelists of 32 men and women who were trained in a sensory evaluation test. The average value was obtained, and the overall preference, taste preference, taste discrimination, and fragrance discrimination were compared by t-test. The test results are shown in Table 22.

Figure 2006204286
Figure 2006204286

上記官能評価試験の結果によれば、実施例3−2のパンは、比較例3のパンに比べ、全ての比較項目において平均点が高く、特に総合嗜好で有意に好まれ、また味識別及び香識別で有意に強くなっていると評価された。この結果から、本発明の冷凍醗酵種生地を用いたパンは、味及び香が著しく改良・増強されたものであることがわかった。   According to the results of the sensory evaluation test, the bread of Example 3-2 has a higher average score in all the comparison items than the bread of Comparative Example 3, and is particularly preferred in the overall taste. The incense identification was evaluated to be significantly stronger. From this result, it was found that the bread using the frozen fermented seed dough of the present invention has significantly improved and enhanced taste and flavor.

実験例3Experimental example 3

本発明として前記実施例4のパンと、比較例4−1として、実施例4における冷凍醗酵種生地を解凍した醗酵種生地の代わりに、前記実施例1−4で作成した液状醗酵種を用いる(パン生地の小麦粉に対して20質量%)ようにして、それ以外は実施例4と同等の原料配合・工程により得たフランスパンと、比較例4−2として、実施例4における冷凍醗酵種生地を解凍した醗酵種生地も、また実施例1−4で作成した液状醗酵種も添加しないようにして、それ以外は実施例4と同等の原料配合・工程により得たフランスパンを用いて、クラム応力測定試験を行い、経時的なパンのクラムの柔らかさを比較・検討した。なお、ベンチタイムは、それぞれの検体において、20分間とった場合と40分間とった場合の2パターンについて、パンを作成した。また、クラム応力として、プランジャーを針入距離だけ一定速度で検体(スライスしたパンクラム)に押圧していったときのパンクラムの応力を測定した。応力が小さいほど、パンクラムが柔らかいことを意味する。   As the present invention, the liquid fermented seed prepared in Example 1-4 is used in place of the fermented seed dough obtained by thawing the frozen fermented seed dough in Example 4 as the bread of Example 4 and Comparative Example 4-1. (20 mass% with respect to the flour of bread dough), and the frozen fermented seed dough in Example 4 as Comparative Example 4-2 and French bread obtained by the same raw material composition and process as in Example 4 In addition to the fermented seed dough that was thawed, and the liquid fermented seed prepared in Example 1-4, other than that, using French bread obtained by the same raw material composition and process as Example 4, crumb A stress measurement test was conducted to compare and examine the softness of bread crumb over time. Bench time was created for each sample in two patterns, 20 minutes and 40 minutes. Further, as the crumb stress, the crumb stress was measured when the plunger was pressed against the specimen (sliced crumb) at a constant speed for the penetration distance. Smaller stress means softer punk crumbs.

Figure 2006204286
Figure 2006204286

測定条件及び測定結果を表23及び図2に示す。図2は、各検体のクラム応力の経時変化を示すものである。実施例4の応力は、製造日の1日後〜3日後のいずれの時点においても最も小さかった。比較例4−1の応力は、製造日の1日後〜3日後のいずれの時点においても実施例4のそれよりもわずかに大きく、この間の応力値の変化は、実施例4のそれと同様の傾向を示した。比較例4−2の応力は、製造日の1日後〜3日後のいずれの時点においても最も大きく、特に、製造日の1日後以降急激に応力の値が大きくなった。   Measurement conditions and measurement results are shown in Table 23 and FIG. FIG. 2 shows changes with time in the crumb stress of each specimen. The stress of Example 4 was the smallest at any time point from 1 day to 3 days after the production date. The stress of Comparative Example 4-1 was slightly larger than that of Example 4 at any time point 1 day to 3 days after the production date, and the change in the stress value during this period was similar to that of Example 4 showed that. The stress of Comparative Example 4-2 was the largest at any time point from 1 day to 3 days after the manufacturing date, and in particular, the stress value increased rapidly after 1 day after the manufacturing date.

この結果から、醗酵種を添加していない比較例4−2のパンが一番硬化が早く進んだのに対し、本発明の醗酵種生地を添加した実施例4のパン及び液状醗酵種を添加した比較例4−1のパンは硬化が遅くなることがわかった。この理由は、乳酸菌醗酵生成物の有する老化抑制及び保湿性向上効果によるものと推測される。本発明の醗酵種生地を添加したパン生地を用いて製造したパンも同様に乳酸菌醗酵生成物の持つ老化抑制及び保湿性向上効果を有することが確認でき、さらに本発明によるパンは、従来の液状醗酵種を添加したパンよりも老化抑制効果が大きいことが確認できた。   From this result, the bread of Comparative Example 4-2 to which no fermented seeds were added progressed most quickly, whereas the bread of Example 4 to which the fermented seed dough of the present invention was added and the liquid fermented seeds were added. It was found that the bread of Comparative Example 4-1 cured slowly. This reason is presumed to be due to the aging inhibition and moisturizing improvement effects of the lactic acid bacteria fermentation product. The bread produced using the bread dough added with the fermented seed dough of the present invention can also be confirmed to have the aging inhibition and moisturizing effect of the lactic acid bacteria fermentation product, and the bread according to the present invention is a conventional liquid fermentation. It was confirmed that the aging inhibitory effect was greater than that of bread with seeds added.

実験例4Experimental Example 4

実施例4、比較例4−1及び比較例4−2のパン生地の抗張力及び伸長力をブラベンダー社製エクステンソグラフを用いて測定し、生地物性への影響を比較・検討した。その結果を以下に示す。   The tensile strength and elongation force of the bread doughs of Example 4, Comparative Example 4-1 and Comparative Example 4-2 were measured using an extender manufactured by Brabender, and the influence on the physical properties of the dough was compared and examined. The results are shown below.

Figure 2006204286
Figure 2006204286

上記Fは抗張力を、Eは伸張度を表すものである。図3縦軸は、これらの比であるF/E値であり、これは、その値が大きいほどパン生地の抗張力が強いか伸展性が少ないことを示すものである。また、図3の横軸は、フロアタイムを20分間とった場合と40分間とった場合の値である。   F represents tensile strength and E represents elongation. The vertical axis in FIG. 3 is the F / E value that is the ratio of these, and this indicates that the higher the value, the stronger the tensile strength of the bread dough or the less the extensibility. The horizontal axis in FIG. 3 is a value when the floor time is 20 minutes and when the floor time is 40 minutes.

この結果から、実施例4及び比較例4−1のパン生地は、比較例4−2のパン生地に比べ、抗張力が小さく、特にフロアタイムを長くとった場合には、この傾向はより顕著となることが確認された。換言すると、醗酵種を添加するとパン生地の伸展性が向上し、伸びやすい生地になるといえる。この理由は、醗酵種中の乳酸がグルテニンの弾性を改変させたためと推測される。この効果は、本発明の冷凍醗酵種生地を用いた場合にも、冷凍しない液状醗酵種を用いる場合と同等であることが確認できた。   From this result, the bread dough of Example 4 and Comparative Example 4-1 has a lower tensile strength than the bread dough of Comparative Example 4-2, and this tendency becomes more prominent particularly when the floor time is increased. Was confirmed. In other words, it can be said that when the fermented seed is added, the extensibility of the bread dough is improved and the dough becomes easy to stretch. The reason for this is presumed to be that lactic acid in the fermented species modified the elasticity of glutenin. Even when the frozen fermented seed material of the present invention was used, it was confirmed that this effect was equivalent to the case of using a liquid fermented seed that was not frozen.

実験例5Experimental Example 5

(生地ファーモグラフ試験)
実施例4、比較例4−1、及び比較例4−2の本捏ミキシング後のパン生地について、それぞれ、ガス発生量をアトー社製ファーモグラフにより測定した。
測定結果を図4に示す。この測定結果から、ガス発生量は、実施例4、比較例4−1及び比較例4−2のパン生地のいずれもほぼ同量であった。従って、本発明の冷凍醗酵種生地を解凍してパン生地に添加しても、パン生地のガス発生量には影響を与えないことが確認できた。
(Fabric farmograph test)
About the bread dough after the main-sheath mixing of Example 4, Comparative Example 4-1, and Comparative Example 4-2, the amount of gas generation was measured with an atto company pharmograph, respectively.
The measurement results are shown in FIG. From this measurement result, the amount of gas generation was almost the same for all of the bread doughs of Example 4, Comparative Example 4-1, and Comparative Example 4-2. Therefore, it was confirmed that even if the frozen fermented seed dough of the present invention was thawed and added to the bread dough, it did not affect the gas generation amount of the bread dough.

図1は、種生地に含まれるアミノ酸の量を示すグラフである。FIG. 1 is a graph showing the amount of amino acids contained in seed dough. 図2は、パンのクラム応力の経時変化を示すグラフである。FIG. 2 is a graph showing the change over time in the crumb stress of bread. 図3は、パン生地の抗張力及び伸長力を示すグラフである。FIG. 3 is a graph showing the tensile strength and elongation force of bread dough. 図4は、パン生地のガス発生量を示すグラフである。FIG. 4 is a graph showing the gas generation amount of bread dough.

Claims (15)

液状醗酵種に少なくとも小麦粉を混合して非流動状の醗酵種生地を作成し、冷凍することを特徴とする冷凍醗酵種生地。   A frozen fermented fermented dough characterized in that at least flour is mixed with a liquid fermented seed to produce a non-fluid fermented seeded dough and frozen. 前記混合して作成した醗酵種生地を、極力醗酵させないように、速やかに冷凍することを特徴とする請求項1記載の冷凍醗酵種生地。   The frozen fermented seed dough according to claim 1, wherein the fermented seed dough prepared by mixing is quickly frozen so as not to ferment as much as possible. 前記混合して作成した醗酵種生地を、分割及び丸め整形してから、冷凍することを特徴とする請求項1又は2記載の冷凍醗酵種生地。   The frozen fermented seed dough according to claim 1 or 2, wherein the fermented seed dough prepared by mixing is divided and rounded and then frozen. 前記液状醗酵種が、少なくとも、小麦粉、水並びに乳酸菌及び/又はイーストから構成されることを特徴とする、請求項1乃至3のいずれか一の請求項記載の冷凍醗酵種生地。   The frozen fermented fermented dough according to any one of claims 1 to 3, wherein the liquid fermented seed is composed of at least wheat flour, water, lactic acid bacteria and / or yeast. 前記液状醗酵種が、醗酵種として直ちにパンの製造に使用できる程度に醗酵させたものであることを特徴とする、請求項1乃至4のいずれか一の請求項記載の冷凍醗酵種生地。   5. The frozen fermented seed dough according to any one of claims 1 to 4, wherein the liquid fermented seed is fermented to such an extent that it can be immediately used for producing bread as a fermented seed. 前記液状醗酵種が、糖類を含むことを特徴とする、請求項1乃至5のいずれか一の請求項記載の冷凍醗酵種生地。   6. The frozen fermented seed dough according to any one of claims 1 to 5, wherein the liquid fermented seed contains a saccharide. 前記液状醗酵種が、種継ぎをしたものであることを特徴とする、請求項1乃至6のいずれか一の請求項記載の冷凍醗酵種生地。   The frozen fermented seed material according to any one of claims 1 to 6, characterized in that the liquid fermented seed has been spliced. 前記液状醗酵種が、該液状醗酵種を構成する小麦粉に対し50〜150質量%の水から作成されることを特徴とする、請求項4乃至7のいずれか一の請求項記載の冷凍醗酵種生地。   The frozen fermented seed according to any one of claims 4 to 7, wherein the liquid fermented seed is prepared from 50 to 150% by mass of water with respect to the flour constituting the liquid fermented seed. Cloth. 前記醗酵種生地が、前記液状醗酵種に対して40〜50質量%の小麦粉を混合して作成することを特徴とする、請求項1乃至8のいずれか一の請求項記載の冷凍醗酵種生地。   The frozen fermented seed dough according to any one of claims 1 to 8, wherein the fermented seed dough is prepared by mixing 40 to 50% by weight of flour with respect to the liquid fermented seed. . 前記請求項1乃至9のいずれか一の請求項記載の冷凍醗酵種生地を解凍してからパン生地の混捏工程で添加することを特徴とするパン生地。   A bread dough, which is added in a kneading step of bread dough after thawing the frozen fermented seed dough according to any one of claims 1 to 9. 前記冷凍醗酵種生地を、前記パン生地を構成する小麦粉に対して3〜30質量%添加することを特徴とする、請求項10記載のパン生地。   The bread dough according to claim 10, wherein 3 to 30% by mass of the frozen fermented seed dough is added to the flour constituting the bread dough. 前記請求項10又は11に記載のパン生地を焼成することを特徴とするパン。   A bread characterized by baking the bread dough according to claim 10 or 11. 前記請求項1乃至9のいずれか一の請求項記載の冷凍醗酵種生地を解凍してからパン生地の混捏工程で添加することを特徴とするパン生地の製造方法。   A method for producing bread dough, wherein the frozen fermented seed dough according to any one of claims 1 to 9 is thawed and then added in a kneading step of bread dough. 前記冷凍醗酵種生地を、前記パン生地を構成する小麦粉に対して3〜30質量%添加することを特徴とする、請求項13記載のパン生地の製造方法。   The method for producing bread dough according to claim 13, wherein the frozen fermented seed dough is added in an amount of 3 to 30% by mass with respect to the flour constituting the bread dough. 前記請求項13又は14に記載の方法により製造されたパン生地を焼成することを特徴とするパンの製造方法。   A method for producing bread comprising baking the dough produced by the method according to claim 13 or 14.
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