JP2006169694A - Method for suppressing rupture of pollen - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a technology for suppressing the rupture of pollen in a nose cavity, the corners of eyes, the surface of conjunctiva or an oral cavity by treating the pollen at the inside or outside of a living environment. <P>SOLUTION: This method for suppressing the rupture of the pollen is characterized by bringing a composition containing an aqueous pectin solution containing a multi-valent metal salt (A), of which turbidity is ≥0.1 measured by a turbidity-measuring method, in contact with the pollen. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for suppressing pollen rupture.

  In recent years, hay fever, which is thought to be caused by mass scattering of cedar pollen and air pollution derived from diesel engine exhaust gas, has become a social problem. Outdoor pollen is brought into the room by opening and closing windows and clothes when going out, and since recently houses are highly airtight, the introduced pollen accumulates. If the allergen elution in the nasal cavity or the like is suppressed with respect to the introduced pollen, it is considered that the comfort in the living environment is further increased.

As a first method for caring for pollen, there is known a method of inactivating a pollen allergen by contacting a specific allergen inactivating component and an allergen substance as described in Patent Documents 1-7.
As a second method for caring for pollen, a method is known in which pollen is destroyed with a specific cationic surfactant to forcibly elute and inactivate allergens as described in Patent Document 8.
As a third method for caring for pollen, as described in Patent Documents 9 to 11, rupture of pollen that has entered the nasal cavity is prevented, and allergen elution is suppressed.

JP 2000-16941 A JP 2002-128680 A JP-A-6-279273 JP 2000-264837 A JP 2003-334240 A JP 2001-247467 A Patent Publication No. 2004-510717 Japanese Patent Laid-Open No. 9-157152 JP-A-7-258070 JP 2000-109425 A JP 2001-240547 A

However, in the first method, even if allergens present on the surface of the pollen can be inactivated by contacting these allergen-inactivating components and pollen, the pollen allergen is also present inside the pollen, The effect of these allergen inactivation methods is inadequate because of the behavior of rupturing and releasing allergens upon contact with nasal discharge.
In the second method, when the treatment is assumed inside and outside the living environment, the sprayed treatment solution dries in a short time, so the two-step process (step 1: rupture pollen, step 2: eluting allergens) It can only be effective in situations where (inactivation) is performed instantaneously.
The third method is considered to be the most effective for the care of pollen inside and outside the living environment. However, all of these publications exhibit an effect by bringing a specific component into contact with the pollen during nasal washing. There was no effective effect on pollen existing outside the nasal cavity.
Therefore, an object of the present invention is to provide a technique capable of suppressing pollen rupture in the nasal cavity, the corner of the eye, the conjunctival surface, or in the oral cavity by treating pollen in and outside the living environment.

  As a result of intensive studies to solve the above problems, the present inventors have clarified that an excellent effect of suppressing pollen rupture can be obtained by treating pollen with a specific polyvalent metal salt. It came.

In order to solve the above problems, the present invention proposes the following means.
A pollen burst inhibiting method characterized by contacting a pollen with a composition containing a water-soluble polyvalent metal salt (A) having a turbidity of a pectin aqueous solution measured by a turbidity measuring method of 0.1 or more. is there.
In the method for suppressing pollen rupture, the composition is preferably sprayed onto pollen adhered to fibers and / or pollen present in the living environment.
In the pollen burst inhibiting method, the polyvalent metal salt (A) is preferably a salt of one or more metals selected from aluminum, zinc, iron, tin, titanium, copper, nickel and cobalt.

  In the present invention, it is possible to provide a technique capable of suppressing pollen rupture mainly in the nasal cavity, the corner of the eye, the conjunctival surface, or in the oral cavity.

  The present invention may be a composition containing a water-soluble polyvalent metal salt (A) having a turbidity of a pectin aqueous solution measured by a turbidity measuring method of 0.1 or more (hereinafter referred to as “the present composition”). ) Is brought into contact with pollen. That is, the polyvalent metal salt (A) acts as a pollen burst inhibitor.

  The present composition can be obtained by adding, mixing and dissolving the component (A) together with water and other optional components.

"Water-soluble polyvalent metal salt (A) having a turbidity of pectin aqueous solution measured by turbidity measurement method of 0.1 or more"
“Water-soluble” means that the amount dissolved in 100 g of water at 25 ° C. is 0.1 g or more. +

“Turbidity of aqueous pectin solution measured by turbidity measurement method” is determined as follows.
2 mL of a 0.2 mass% polyvalent metal salt aqueous solution is added to 2 mL of a 1 mass% pectin aqueous solution at 25 ° C. and stirred.
The product name: GENU pectin type LM-102AS-J [manufactured by Genue Co., Ltd.] is used for pectin.
4 mL of distilled water is added to this solution, and the absorbance at 660 nm, that is, turbidity is measured using a turbidimeter. The turbidimeter uses the product name: SPECTRONIC 20+ (Spectronic Instruments). As the measurement container, a test tube having an outer diameter of about 18 mm and an inner diameter of about 16 mm is used.
In the measured value of turbidity, pure water is used as a blank, the turbidity in that case is set to 0, and the turbidity in a state where the test tube is taken out and shielded from light is set to infinity.
The turbidity of the aqueous polyvalent metal salt solution obtained by the above method is referred to as “turbidity of aqueous pectin solution measured by turbidity measurement method” (hereinafter abbreviated as “turbidity”).

  In the present invention, the higher the turbidity of the component (A), the higher the effect of suppressing pollen bursting, which is preferable. Turbidity needs to be 0.1 or more, more preferably 0.2 or more, and still more preferably 0.4 or more. The upper limit is not particularly limited, but is substantially 2 or less (preferably 2.0 or less).

The component (A) is preferably one or more metal salts selected from aluminum, zinc, iron, tin, titanium, copper, nickel and cobalt. Examples of the counter ion forming the salt include Cl ⁻ and SO ₄ ²⁻ .
Among these, aluminum, zinc, and iron salts are preferable. For example, aluminum chloride, aluminum sulfate, alum, zinc chloride, zinc sulfate, iron (II) chloride, iron (III) chloride, iron (II) sulfate, iron sulfate ( III), and the like.
Particularly preferred are aluminum salts, such as aluminum chloride, aluminum sulfate and aluminum alum, with aluminum chloride being preferred.
(A) A component can be used 1 type or in mixture of 2 or more types.

  The compounding quantity in the composition of (A) component is 0.01-40 mass%, for example, Preferably it is 0.1-10 mass%, More preferably, you may be 0.5-5 mass%. An effect improves by setting it as more than a lower limit. By making it below the upper limit, it is advantageous in terms of cost.

"Optional ingredients"
In addition to water and the component (A), the composition may contain optional components as exemplified below.
The pH of the composition is preferably neutral or acidic from the viewpoint of the effect, preferably in the range of pH 1 to 7, more preferably 3.0 to 6.0, particularly 4.0 to 6.0. is there. By setting it within this range, the component (A) tends to act uniformly on the pollen.
The pH can be adjusted by using one or more pH adjusting agents such as sulfuric acid and sodium hydroxide (usually used in an aqueous solution).
In addition to this, optional components that can be blended in the present composition are exemplified below. Each component can be used alone or in combination.
However, a chelating agent having a high chelating ability such as citric acid and EDTA (ethylenediaminetetraacetic acid) may reduce the effect of the present invention. Therefore, it is preferably blended to such an extent that it does not adversely affect. More preferably, it is not contained in the present composition.
(1) Nonionic, cationic, anionic surfactant (2) Silicone compound, modified silicone compound (3) Polyethylene glycol (4) Films of lower alcohols such as methanol, ethanol, propyl alcohol, isopropyl alcohol (5) Glycol solvents Anti-formation agents (6) Low-temperature stabilizers such as sodium paratoluenesulfonate, sodium cumenesulfonate, ethanol, etc. (7) Anti-contamination agents such as carboxymethylcellulose and polyethyleneimine derivatives (8) Proteases, lipases, amylases, cellulases, etc. Enzyme preparation (9) Fluorescent agent such as distilbiphenyl type fluorescent agent (10) Antioxidant such as dibutylhydroxytoluene (11) Antibacterial agent such as caisson CG / ICP (12) Solvent and stabilizer Fragrance such as fragrance composition

  In the present invention, the present composition containing the component (A) is brought into contact with pollen and preferably dried. Then, even if pollen is taken into the body, rupture of the pollen in the nasal cavity, the corner of the eye, the conjunctival surface, or the oral cavity can be suppressed.

Application example
The pollen rupture suppression method can be applied to various scenes using cosmetics, quasi-drugs, pharmaceuticals, etc. in addition to miscellaneous goods such as detergents and fiber treatment agents.
Moreover, the to-be-processed object which is a target which processes the pollen which adhered etc. may exist in the living environment or what exists outside a living environment. Here, the living environment refers to an entire space corresponding to a closed space where a person exists, and includes a so-called house (house, building, etc.), a car, and the like.
Specifically, when it is applied in a living environment, the target includes all things such as spraying on the walls and floors constituting the living environment (residence), spraying on textile products, furniture, toys, stuffed animals, etc. . Moreover, in order to process the floating pollen in the air, it can be sprayed in the space in the living environment.
When applied outside the living environment, the targets include direct spraying on cedar and cypress males, spraying from cedar and cypress forests from a helicopter. When sprayed on standing trees such as cedar and mountain forests, it can also be used for the purpose of suppressing pollination.
Moreover, in order to process floating pollen in the air such as Pollen Cloud on a windy day, it can be sprayed in a space outside the living environment.

(A) As a method of bringing the composition containing the component into contact with pollen, specifically,
(I) The method of spraying this composition with respect to pollen (ii) The method of spraying this composition to the place where pollen adhered (iii) The method of spraying to the plant which attached pollen etc. are mentioned.
Also,
(IV) The present composition may be brought into contact with pollen during washing.
For example, (A) component or this composition in which (A) component is dissolved in the cleaning liquid, and a method of washing, and the rinse liquid is added with this composition in which (A) component or (A) is dissolved and rinsed. The method etc. are mentioned.

More specifically, as the above (i), there is a method in which the present composition is packed in a spray such as an aerosol or a dispenser container and sprayed onto pollen. More specifically, for example, by deodorizing agents, antibacterial / antifungal agents, insecticides, mite repellents, acaricides, dehumidifiers, air spray agents, and fragrances, and spraying them. is there.
As (ii), the present composition is adhered to or absorbed by a cleaning tool such as nonwoven fabric, paper, woven fabric, cloth product, sheet, sponge, cotton swab, mop, etc., and the method is used to wipe the object. A method of blending the present composition containing the component (A) in the liquid and wiping the object using the composition is mentioned.
(Iii) As a method of spraying the composition from a helicopter to a cedar forest or a cypress forest, or when growing seedlings of flowering trees, the composition is sprayed on a specific plant in order to control pollination A method is mentioned.
As the (iV), the present composition containing the polyvalent metal salt (A) is blended with a detergent, a rinsing agent, a fiber treatment agent, a softening agent, or the like used for washing. The method of performing is mentioned.

In the present invention, it is particularly effective and preferable to spray pollen adhered to fibers and / or pollen present in the living environment.
For example, it is preferable to use the present composition containing a polyvalent metal salt (A) in a textile product or a spray product for living environment.

  Moreover, this composition can also be used by making it adhere or impregnate to filters, masks, etc., such as an air conditioner, an air cleaner, a dehumidifier, a dryer, and a ventilator. Since the present composition may be dried on the surface of the above filter, mask, etc., it can be used in the air by using a device that replenishes water and / or hygroscopic substances and / or liquid such as moisture. It is preferable that a liquid such as moisture or moisture is replenished to a filter, a mask or the like.

  The present invention is particularly suitable for the treatment of cedar and cypress pollen.

Although the action which can suppress the burst of pollen is not certain, it can be estimated as follows.
The pollen shell is formed from a plurality of layers, among which there is a layer containing pectin. The shell has a gap for water to enter. It is conceivable that when moisture penetrates into the pollen, the inside expands and the shell is torn to expose the inside of the pollen, that is, to burst. When pollen bursts, allergens inside the shell are released.
However, pollen does not explode easily even if it is introduced into pure water. However, pollen easily bursts when placed in body fluids such as nasal discharge. Pure water is unlikely to enter the pollen shell, and body fluids such as nasal discharge can easily enter the inside through the gaps in the shell.
The component (A), which increases its turbidity when reacted with pectin, acts on pectin present in the pollen shell and is considered to crosslink between pectin. And it is thought that the strong pectin layer is formed by the bridge | crosslinking, the shell becomes firm, and the rupture of pollen is prevented.

  Hereinafter, the present invention will be described in more detail using examples, but the present invention is not limited to these examples. The unit “%” for the composition is “% by mass” unless otherwise specified.

(Test Examples 1-12, Comparative Test Examples 1-3)
The turbidity of various polyvalent metal salts shown in Table 1 was measured. The results are shown in Table 1.
In the polyvalent metal salt, no difference was observed in the turbidity even when the type of anion was changed.

(Examples 1-6, Comparative Examples 1-3)
From the results shown in Table 1, those having a turbidity of 0.1 or more (Examples 1 to 6) and those having a turbidity of 0.1 or less (Comparative Examples 1 to 3) were separated. And about the various polyvalent metal salt shown in Table 2, water was added and it was set as the composition of concentration 1%, and this was made into the "evaluation sample." The cedar pollen was collected from cedar grown in Minamiashigara City, Kanagawa Prefecture. And the burst suppression rate of the cedar pollen was calculated | required with the following method. The results are shown in Table 2.
In the polyvalent metal salt, no difference was observed in the burst inhibition rate even when the type of anion was changed.

"Evaluation of cedar pollen burst suppression effect"
The pollen burst inhibiting effect was evaluated according to the following method.

100 mg of cedar pollen was added to 10 mL of the “evaluation sample”, allowed to stand at room temperature for 5 minutes, and then centrifuged at 300 rpm for 5 minutes. The precipitate was taken out and further filtered, and the pollen was dried at 25 ° C. and 60% humidity for 15 hours.
Contains artificial nasal discharge (Na ⁺ (0.2678%) + K ⁺ (0.1%) + Ca ²⁺ (0.003%) + Mg ²⁺ (0.0015%) + Cl ⁻ (0.5606%) to pH 9 with ammonia Adjusted aqueous solution) Add about 0.6 mg of this treated pollen to 0.5 mL and leave it at 37 ° C. for 5 minutes. At this time, determine the number of pollen that has been ruptured and the number of pollen that has not been ruptured. Measured.
The number of pollen was measured by taking an enlarged photograph (200 times) of the evaluation sample with a microscope and visually measuring it. As the microscope, VH-7000 (product name) [manufactured by Keyence Corporation] was used.
From the number of pollen measured as described above, the proportion of pollen that was not ruptured was calculated based on the following formula.
Pollen rupture inhibition rate (%) = (number of pollen that has not been ruptured / (number of pollen that has been ruptured + number of pollen that has not been ruptured)) × 100

From the results in Table 2, it was confirmed that the examples of the present invention have a high effect of suppressing pollen bursting.
In addition, pollen rupture was suppressed even in an actual runny nose, and the same effect was obtained also in the nasal cavity.

(Examples 7 to 9, Comparative Example 4)
From the results shown in Table 2, various polyvalent metal salts having different rupture inhibition rates were selected. And with the compounding composition shown in Table 3, while adding the balance amount of water, the material was mixed and this was made into the "evaluation sample." In Table 3, the blending unit is “mass%”, and the total blending amount is 100 mass%. And the burst inhibition rate of the cedar pollen was calculated | required by said "evaluation of the burst inhibition rate of a cedar pollen" method. The results are shown in Table 3.
Evaluation criteria: ○ when the burst inhibition rate is 70% or more, Δ when the burst inhibition rate is 40% or more and less than 70%, and × when the burst inhibition rate is less than 40%.

* 1 "Polyether silicone": a laboratory synthetic product with a structure represented by the following chemical formula

From the results in Table 3, it was confirmed that the Examples of the present invention have a high effect of suppressing pollen bursting.
In addition, pollen rupture was suppressed even in an actual runny nose, and the same effect was obtained also in the nasal cavity.

Claims (3)

  A method for inhibiting pollen rupture, comprising bringing a composition containing a water-soluble polyvalent metal salt (A) having a turbidity of a pectin aqueous solution measured by a turbidity measurement method of 0.1 or more into contact with pollen.   The pollen burst inhibiting method according to claim 1, wherein the composition is sprayed onto pollen adhered to fibers and / or pollen present in a living environment. In the pollen burst inhibiting method according to claim 1 or 2,
The pollen burst inhibiting method, wherein the polyvalent metal salt (A) is a salt of one or more metals selected from aluminum, zinc, iron, tin, titanium, copper, nickel and cobalt.