JP2006160891A - Detergent composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a detergent composition which is excellent in removability of mud stain deposited on clothes etc., especially excellent in decomposition and removability of hardly decomposable corrosive substances contained as colored components. <P>SOLUTION: The detergent composition contains a pectinase, manganese peroxidase and a hydrogen peroxide source. In a preferred embodiment, the hydrogen peroxide source is at least one chosen from hydrogen peroxide, a hydrogen peroxide-producing compound and a hydrogen peroxide-producing enzyme, or a manganese compound is further contained. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a cleaning composition excellent in the removal of dirt on hard surfaces such as fabrics, earthenware, and glass, in particular, the effect of removing mud dirt adhered to clothing.

Enzymes are used in various fields such as food processing and pharmaceuticals. In particular, there are many examples of use in cleaning compositions as represented by detergents for clothing.
Dirt substances that are the targets of such cleaning agents are roughly classified into dirt derived from human metabolites such as sebum and dirt derived from living environments such as mud and pigment components. .
For the purpose of removing these soil substances, a surfactant is usually added to the cleaning composition, but an enzyme is added to the cleaning composition to further enhance the soil removal effect. Many attempts have been made.
For example, a detergent composition containing pectinases alone or in combination with a detergency enhancing factor other than enzymes has been proposed for the purpose of decomposing colored components in fruit juice and mud soil (see Patent Document 1). .
In addition, for the purpose of redox decomposition of a colored product, a cleaning composition comprising oxidoreductases alone and in combination with a cleaning composition or a detergency enhancing factor other than an enzyme has been proposed (see Patent Document 2). ). However, in this proposal, the effect of washing and removing mud dirt adhering to clothing etc. was not sufficient.

In addition, a detergent composition has been proposed in which a plurality of enzyme groups including pectinase and oxidoreductase are combined as a detergent component that can be combined (see Patent Document 3). Further, a detergent composition containing an oxidoreductase has been proposed, and the effect of dye decomposition is also described (see Patent Documents 4 and 5).
However, even in these proposals, if only one of pectin-degrading enzyme and oxidoreductase is used alone, the detergency against mud soil is not sufficient, and further improvement is required.

Therefore, the applicant of the present application first washed pectin degrading enzyme and oxidoreductase in combination with stabilized washing against mud dirt such as clothing as compared with the case where pectin degrading enzyme and oxidoreductase are used alone. A cleaning agent component with improved power is proposed (see Patent Document 6).
However, even in this proposal, the cleaning and removing effect for removing mud dirt adhering to clothing and the like is not sufficiently satisfactory, and further improvement and development are desired at present.

Japanese Patent Publication No. 6-39596 JP-A-6-234997 Japanese National Patent Publication No. 11-505562 Japanese National Patent Publication No. 11-508136 Japanese National Patent Publication No. 11-511882 Japanese Patent Laid-Open No. 2003-9856

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, an object of the present invention is to provide a cleaning composition that is excellent in removing mud stains adhering to clothing and the like, and particularly excellent in the decomposition and removal of hardly decomposable corrosive substances as coloring components.

  In order to solve the above-mentioned problems, the present inventors have conducted intensive studies. As a result, by combining a pectin-degrading enzyme, a manganese peroxidase, and a hydrogen peroxide source, these can be used alone, and pectin-degrading can be performed. It has been found that it has a higher cleaning effect and is particularly useful for removing mud soil as compared with the case where an enzyme is used in combination with an oxidoreductase other than manganese peroxidase which has been studied in the past.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> A detergent composition comprising a pectin-degrading enzyme, manganese peroxidase, and a hydrogen peroxide source.
<2> The cleaning composition according to <1>, wherein the hydrogen peroxide source is at least one selected from hydrogen peroxide, a hydrogen peroxide generating compound, and a hydrogen peroxide producing enzyme.
<3> The cleaning composition according to any one of <1> to <2>, further containing a manganese compound.

  According to the present invention, it is possible to solve the above-mentioned problems and to provide a cleaning composition excellent in removal of mud dirt adhering to clothing and the like, and particularly excellent in decomposition and removal of hardly decomposable corrosive substances as coloring components. Can be provided.

  The cleaning composition of the present invention contains a pectin degrading enzyme, manganese peroxidase, and a hydrogen peroxide source, and further contains a manganese compound and other components as necessary.

-Pectin-degrading enzyme-
Examples of the pectin-degrading enzyme include the following three embodiments.
As a 1st aspect, the enzyme which hydrolyzes alpha-1,4 glycosidic bond of galacturonic acid called polygalacturonase is mentioned.
As a second embodiment, a pectin lyase that hydrolyzes an α-1,4 glycosidic bond of galacturonic acid and generates a double bond between C4 and C5 at the non-reducing end of uronic acid, which is the degradation product, The enzyme called is mentioned.
The enzyme shown in the first aspect and the second aspect is further divided into two depending on whether the substrate is pectin or pectic acid, and depending on whether it acts on the end or the inside of the substrate, 2 minutes.
A third embodiment includes an enzyme called pectinesterase that degrades the methyl ester of galacturonic acid.
These pectin-degrading enzymes may be used alone or in combination of two or more.

The titer of the pectin-degrading enzyme is 10 U / g or more when the force for generating 1 μmol of pectin-degrading product from the substrate by the pectin-degrading enzyme is 1 U regardless of the composition ratio of the pectin-degrading enzyme. Is preferably 100 U / g or more.
If the titer is less than 10 U / g, the effect of removing mud stains in clothing or the like may not be sufficient.

There is no restriction | limiting in particular as content in the said detergent composition of the said pectin degrading enzyme, According to the objective, it can select suitably, 0.01-30 mass% is preferable, 0.1-10 mass% Is more preferable.
If the content is less than 0.01% by mass, the effect of removing mud stains in clothing and the like may not be sufficient, and if it exceeds 30% by mass, the object to be cleaned such as clothing may be colored. is there.

  There is no restriction | limiting in particular as pH in the said pectin degradation enzyme, According to the objective, it selects suitably from acidic, neutral, and alkaline pH range.

  The origin of the pectin-degrading enzyme is not particularly limited, and those widely distributed in plants, bacteria, fungi and the like can be used.For example, bacteria such as Bacillus genus, Cytophaga genus, Pseudomonas genus, Trichosporon, Endomyces, Endomycopsis, Saccharomyces, Schizosaccharomyces, Pichia, Hansenula, Debaryomyces, Hansenia spora, Tolropsis, Candida, Kluyveromyces and other yeasts, Fusarium, Examples thereof include filamentous fungi such as Galactomyces genus, Aspergillus genus, Rhizopus genus, Trametes genus, enzymes present in fruit peels such as oranges, and the like.

Preferred examples of the pectin-degrading enzyme include the following commercially available pectinases using the above-mentioned various bacteria. Specifically, sucrase S, sucrase N (manufactured by Sankyo Corporation), ultrapectinex (manufactured by Novozymes) Pectinase Tanabe (manufactured by Tanabe Seiyaku Co., Ltd.), pectinase A (manufactured by Amano Enzyme Co., Ltd.), pectinase (manufactured by Shengshin Pharmaceutical Co., Ltd.), pectinase (manufactured by Nagase Sangyo Co., Ltd.), pectinase SS (manufactured by Yakult Co., Ltd.), etc. Can be mentioned.
As the pectin-degrading enzyme, a pectinase at the research reagent level sold by Sigma or the like can also be used.
Furthermore, as the pectin-degrading enzyme, an enzyme produced using a gene recombinant in which the enzyme gene is incorporated into an appropriate plasmid can also be used.
The pectin-degrading enzyme is not limited to these commercially available products, and semi-purified or crude pectinase can be used as long as the effects in the present invention are not impaired.

-Manganese peroxidase-
The manganese peroxidase is an oxidoreductase having hydrogen peroxide as an acceptor. The manganese peroxidase activates divalent manganese ions, which are mediators, into trivalent manganese ions in the presence of hydrogen peroxide, and the activated mediator oxidizes various target substances.
The titer of the manganese peroxidase is preferably 1 U / g or more when the force for generating 1 μmol of a redox product from the substrate by the manganese peroxidase is 1 U. The above titer is more preferable. If the titer is less than 0.1 U / g, the effect of removing mud stains in clothing or the like may not be sufficient.

There is no restriction | limiting in particular as content in the said detergent composition of the said manganese peroxidase, According to the objective, it can select suitably, For example, 0.01-30 mass% is preferable, 0.1-10 The mass% is more preferable.
If the content is less than 0.01% by mass, the effect of removing mud stains may not be sufficient, and if it exceeds 30% by mass, the object to be cleaned such as clothing may be colored.

  The origin of the manganese peroxidase is not particularly limited, and those widely distributed among microorganisms, bacteria, and fungi can be used. For example, the genus Bacillus, Streptomyces, Seripoliopsis, and Dicomitas can be used. Genus, Flavia, Phynoporus, Fanerokete, Tyrospora, Penicillium, Funaria, Lamicrodium, Schizophyllum, Nematroma, Lentinas, Cortinaris, Coriolus, Pleurotas, Ganoderma, Aspergillus, Trametes And microorganisms such as, and mushrooms.

As said manganese peroxidase, the commercially available manganese peroxidase which uses these various origin bacteria can be used. In addition, as the manganese peroxidase, an enzyme produced using a gene recombinant in which an enzyme gene is incorporated into an appropriate plasmid can also be used.
The manganese peroxidase is not limited to these commercially available products, and semi-purified or crude manganese peroxidase can be used as long as the effects in the present invention are not impaired.

  The constituent ratio of the pectin degrading enzyme and the manganese peroxidase is not particularly limited and can be appropriately selected according to the purpose. ) Is preferably mixed in the range of 100: 1 to 1: 100.

  By combining the pectin-degrading enzyme and the manganese peroxidase, these are used alone, and higher than when using a combination of a pectin-degrading enzyme and an oxidoreductase other than manganese peroxidase, which has been conventionally studied. It has a cleaning effect and is particularly useful for removing mud dirt.

-Hydrogen peroxide source-
The hydrogen peroxide source is not particularly limited as long as it can generate hydrogen peroxide, and can be appropriately selected according to the purpose. For example, the hydrogen peroxide source is hydrogen peroxide, hydrogen peroxide Preference is given to at least one selected from generating compounds and hydrogen peroxide producing enzymes.
Examples of the hydrogen peroxide include aqueous hydrogen peroxide. Examples of the hydrogen peroxide generating compound include percarbonates such as sodium percarbonate, perborates, peroxycarboxylic acids or salts thereof. Examples of the hydrogen peroxide producing enzyme include glucose oxidase, alcohol oxidase, cholesterol oxidase and the like.

  The content of the hydrogen peroxide source in the cleaning composition is not particularly limited and may be appropriately selected according to the purpose. The manganese peroxidase is activated as the amount of hydrogen peroxide during cleaning. Therefore, 0.1 to 1000 ppm is preferable, and 1 to 100 ppm is more preferable.

-Manganese compounds-
There is no restriction | limiting in particular as said manganese compound, According to the objective, it can select suitably, For example, manganese sulfate, manganese chloride, manganese carbonate, manganese acetate, etc. are mentioned.
The manganese compound is a compound that generates divalent manganese ions under the use conditions of the cleaning composition, and the divalent manganese ions act as a mediator of the manganese peroxidase and are trivalent by manganese peroxidase. It is activated by being oxidized to manganese ions, and various target substances are oxidized by the trivalent manganese ions.
Since the manganese compound is contained in a large amount in the soil, it is considered that the manganese compound is supplied from mud dirt or the like adhering to clothing and the like. When used in combination with the composition, the cleaning ability of the cleaning composition may be improved.
There is no restriction | limiting in particular in the addition amount in the said cleaning composition of the said manganese compound, According to the objective, it can select suitably, 0.0001-0.1 mass% is preferable.

-Other ingredients-
In the cleaning composition of the present invention, conventionally known substances can be added as the other components as necessary, for example, enzyme enhancers, surfactants, builders, etc. Can be mentioned.

  The enzyme enhancer is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include halide ions of chlorine and bromine, p-hydroxycinnamic acid, 2,4-dichlorophenol, vanillin, and 7-hydroxycoumarin. Phenols such as 6-hydroxy-2-naphthoic acid and p-hydroxybenzenesulfonate; 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonate: ABTS), 10-methylphenothiazine, 10-ethylphenothiazine 10-propylphenothiazine, 10-phenothiazinepropionic acid (PPT), 10-ethylphenothiazine-4-carboxylic acid (EPC), 10-phenoxazinepropionic acid (POP), 10-methylphenoxazine, and the like. These may be used alone or in combination of two or more.

Suitable examples of the surfactant include conventionally known anionic surfactants, nonionic surfactants, and amphoteric surfactants.
These may be used alone or in combination of two or more.
Moreover, as content in this detergent composition in this surfactant, about 5-50 mass% is preferable.

There is no restriction | limiting in particular as said anionic surfactant, According to the objective, it can select suitably, For example, the following are used suitably.
(1) A linear or branched alkylbenzene sulfonate having 8 to 16 carbon atoms.
(2) Alkyl sulfate or alkenyl sulfate having a hydrophobic group having 10 to 20 carbon atoms.
(3) Alpha-olefin sulfonate having a hydrophobic group having 10 to 20 carbon atoms.
(4) C8-20 saturated or unsaturated alpha-sulfo fatty acid ester salts.
(5) It has a C10-20 linear or branched alkyl group or alkenyl group, and averages one kind of alkylene oxide such as ethylene oxide (EO), propylene oxide (PO), butylene oxide (BO), etc. Alkyl sulfate added at 0.5 to 8 moles or an average of 0.5 to 8 moles of EO and PO in a molar ratio of 0.1 / 9.9 to 9.9 / 0.1.
(6) Ether carboxylates having a linear or branched alkyl group or alkenyl group having 10 to 20 carbon atoms and having an average of 2 to 20 moles of ethylene oxide added thereto.
(7) A sulfosuccinic acid ester type anionic surfactant having a linear or branched alkyl group or alkenyl group having 10 to 20 carbon atoms.
There is no restriction | limiting in particular as a salt of the said anionic surfactant, According to the objective, it can select suitably, For example, a sodium salt, potassium salt, an alkanolamine salt, etc. are mentioned.
Moreover, beef tallow or a palm-derived fatty acid salt can be added to the anionic surfactant.

There is no restriction | limiting in particular as said nonionic surfactant, According to the objective, it can select suitably, For example, the following are mentioned.
(1) Polyethylene oxide alkyl, alkenyl ether, polyethylene oxide polyoxypropylene alkyl, alkenyl ether, polyethylene oxide alkylphenyl ether having an alkyl group having 10 to 18 carbon atoms and having an average addition number of ethylene oxide (EO) of 2 to 30 , Polyethylene oxide alkylalkylamines.
(2) A methoxypolyethylene oxide alkanoate having an alkyl group having 10 to 20 carbon atoms and having an ethylene oxide (EO) average addition mole number of 1 to 30.
(3) A higher fatty acid alkanolamide having 10 to 20 carbon atoms.
(4) Alkyl polyglucoside having an alkyl group having 8 to 18 carbon atoms and having an average addition mole number of 1 to 3 glucose units.
(5) Alkyl glyceryl ether having an alkyl group having 10 to 14 carbon atoms and having an average addition mole number of 1 to 3 glycerin units.
(6) Alkylamine oxide, alkylamidoamine oxide, alkylhydroxyamine oxide.

  The amphoteric surfactant is not particularly limited and may be appropriately selected depending on the intended purpose. For example, carboxybetaine, aminocarboxylate, alkylsulfobetaine, hydroxyalkylsulfobetaine, alkylimidazolinium betaine, alkylbetaine, alkyl And amidopropyl betaine.

There is no restriction | limiting in particular as said builder, According to the objective, it can select suitably, For example, crystalline aluminosilicates, such as A-type zeolite; Phosphate, such as a tripolyphosphate; Ethylenediamine tetraacetate, nitrilotriacetate, etc. Aminopolyacetates; amino acids such as aspartic acid, glutamic acid, glycine; salts such as sulfonated carboxylic acids; salts of dicarboxylic acids such as oxalic acid, malonic acid, succinic acid, glutaric acid, adipic acid; glucholic acid, malic acid, Salts of oxycarboxylic acids and organic acids such as tartaric acid, acetic acid, citric acid, and gluconic acid, and derivatives thereof; pyromettes such as itaconic acid, maleic acid, fumaric acid, and aconitic acid; reducing alkali metal salts such as sodium sulfite; Acid salt, carbonate, triethanolamine, diethanolamine, triisopropanolamine And the like of the alkali agent and an inorganic electrolyte.
As content in the said cleaning composition of this builder, about 1-50 mass% is desirable.

  In addition, if necessary, bleaching agents such as sodium percarbonate and sodium perborate monohydrate, bleach activators, enzymes such as protease, lipase, cellulase, amylase, laccase and oxidase, and increased amounts of sodium sulfate and the like Agents, polyethylene glycol (PEG), polyvinyl pyrrolidone (PVP) and polyvinyl alcohol (PVA), dispersants such as amines and amphoteric polymers, or color transfer inhibitors, boron compounds and enzyme stabilizers such as sodium sulfite, silicone / silica Known components such as antifoaming agents, antioxidants, oil-absorbing carriers, bluing agents, fragrances, and the like can be used in known addition amounts.

The manufacturing method of the said cleaning composition is not specifically limited, The manufacturing method of various conventionally well-known cleaning composition can be used.
Moreover, there is no restriction | limiting in particular as a shape of the said cleaning composition, Various shapes, such as a granular form, a powder form, a liquid form, and a paste form, are mentioned.
In addition, the cleaning composition is used for washing clothes, starting with use under normal washing conditions such as a washing machine, pre-soaked washing, application washing, etc., and further under conditions such as hard surface washing. It can be suitably used in a wide range of applications such as use of

According to the present invention, a detergent composition having excellent detergency can be obtained. Specific examples will be shown below to explain the effects of the present invention. However, the present invention is not limited to the following examples.
In the examples, “%” means “% by mass” unless otherwise specified.

<Experiment 1>
(1) Preparation of cleaning composition Each cleaning composition was prepared from the compositions shown in Tables 1 and 2 by a conventional method.
(2) Preparation of mud artificial soil cloth Black ink for gardening is dried, pulverized and classified, and 1% is obtained by ultrasonically dispersing mud particles having a volume average particle diameter (D ₅₀ ) of 1 to 10 μm in ion-exchanged water. A suspension was prepared by adding clay as a main component of kaolinite, vermiculite, etc., which are crystalline minerals, dried at 200 ° C. for 30 hours as inorganic soil. After immersing a cotton knitted cloth (manufactured by Tanigami Shoten Co., Ltd.) in this dirt bath, water was squeezed with two rubber rolls to uniformize the amount of dirt adhered. This cloth was dried at 105 ° C. for 30 minutes, and then the cloth from which excessively adhered dirt was removed by brushing was cut into 5 cm × 5 cm and used for evaluation as a model mud-contaminated cloth.
In the preparation of mud artificial soil cloth, mud by the cleaning composition of the present invention is used by using black soil containing a lot of organic components, which are hardly decomposable corrosive substances, as a coloring component, instead of red soil containing a lot of inorganic components. In addition to the removal effect of dirt, the decomposition and removal effect of corrosive substances were evaluated.

(3) Detergency test Using Terg-0-meter (manufactured by U.S. Testing), 10 sheets of the above-mentioned contaminated cloth and knitted cloth were added, and the bath ratio was adjusted to 20 times, and prepared in (1). The detergent composition was added and washed at 30 ° C., 120 rpm for 10 minutes. Thereafter, after rinsing and drying, the reflectance was measured to determine the cleaning power (cleaning rate). In this case, 900 mL of cleaning solution having a cleaning agent concentration of 0.066% was used, and rinsing was performed twice with 900 mL of water for 5 minutes. The water used had a hardness of 3 ° DH.

(4) Evaluation method The cleaning rate (%) was determined by the Kubelka-Munk formula expressed by the following formula 1. In addition, evaluation of the detergency was performed by an average value of 10 test cloths. The standard white cloth is a cotton knitted cloth (manufactured by Tanigami Shoten Co., Ltd.) washed with a cleaning composition having the composition shown in Table 1 (without enzyme).

ただし、前記数式中、Ｒは日本電色（株）製の色彩計Σ−９０００を用いて測定した反射率である。

However, in the said numerical formula, R is the reflectance measured using the Nippon Denshoku Co., Ltd. colorimeter (SIGMA) -9000.

(Use composition component)
The meanings and details of the abbreviations in Tables 1 to 6 are as follows, and the mass% values in the tables are values in terms of pure content. In addition, EOp shows the average added mole number of ethylene oxide, and POp shows the average added mole number of propylene oxide.
LAS: Sodium alkylbenzene sulfonate having 10 to 14 carbon atoms α-SF: Sodium α-sulfo fatty acid (carbon number 14 to 16) AOS: Sodium α-olefin sulfonate having 14 to 18 carbon atoms Soap: Carbon number 12-18 palm oil-derived fatty acid sodium AE: C12 alcohol ethoxylate (EOp = 12)
AEP: EO, PO adduct of alcohol having 13 carbon atoms (EOp = 12, POp = 3)
Copolymer: Sodium salt of a copolymer having a molar ratio of acrylic acid and maleic acid of 60:40 and an average molecular weight of 50,000 (Germany; manufactured by BASF, trade name Socalan CP7)
Zeolite: Type 4A zeolite (average particle size 1.2 μm)
Fluorescent agent: 4.4-bis (sulfostyryl) biphenyl disodium / 4.4-bis {(4-anilino-6-monophorino-1,3,5-triazin-2-yl) amino} stilpene-2,2- Disulfone disodium = 1/1 mixture (trade name Chino Pearl CBS / trade name Chino Pearl AMS)
Hydrogen peroxide solution: 30% hydrogen peroxide content

(Enzyme used)
Enzymes “A” to “F” listed in Tables 1 to 6 are as follows.
A: Sucrase S (manufactured by Sankyo Corporation)
B: Pectin lyase (manufactured by Sigma)
C: Laccase (manufactured by Koken)
D: Glucose oxidase (manufactured by Sigma)
E: Peroxidase (Wako Pure Chemical Industries, Ltd.)
F: Manganese peroxidase (manufactured by Jena Bioscience)
Further, at the time of blending manganese peroxidase, 0.017% of manganese sulfate was added to 100% of the detergent composition.

※洗浄力は、酵素なしの場合（対照）の洗浄力を１００％として相対値で示す。

* Detergency is shown as a relative value when the detergency without enzyme (control) is 100%.

※洗浄力は、酵素なしの場合（対照）の洗浄力を１００％として相対値で示す。

* Detergency is shown as a relative value when the detergency without enzyme (control) is 100%.

  From the results of Tables 1 and 2, it was found that the combination of a pectin degrading enzyme, manganese peroxidase, and a hydrogen peroxide source showed a high detergency against mud soil adhering to clothing.

<Experiment 2>
Three mud artificial dirt cloths were prepared in the same manner as in the above <Experiment 1> except that each cleaning composition was prepared from the components shown in Tables 3 and 4 by a conventional method.
The cleaning power was evaluated in the same manner as in the above <Experiment 1> using the obtained three artificial mud dirt cloths.

※洗浄力は、酵素なしの場合（対照）の洗浄力を100％として相対値で示す。

* Detergency is expressed as a relative value when the detergency without enzyme (control) is 100%.

※洗浄力は、酵素なしの場合（対照）の洗浄力を１００％として相対値で示す。

* Detergency is shown as a relative value when the detergency without enzyme (control) is 100%.

  From the results of Table 3 and Table 4, even in typical liquid detergent compositions, a combination of pectin-degrading enzyme, manganese peroxidase, and hydrogen peroxide source provides high detergency against mud dirt adhering to clothing. It turns out to show.

<Experiment 3>
After preparing each cleaning composition from the composition shown in Table 5 and Table 6 by a conventional method, applying it to three mud artificial dirt cloths prepared in the same manner as in the above <Experiment 1>, As in <Experiment 1>, using a Terg-0-meter (manufactured by U.S. Testing), a commercially available laundry detergent (trade name: Top (manufactured by Lion Corporation)), 25 ° C., 120 rpm Washed for 10 minutes. Thereafter, after rinsing and drying, the reflectivity was measured to evaluate the cleaning power.

※洗浄力は、酵素なしの場合（対照）の洗浄力を１００％として相対値で示す。

* Detergency is shown as a relative value when the detergency without enzyme (control) is 100%.

※洗浄力は、酵素なしの場合（対照）の洗浄力を１００％として相対値で示す。

* Detergency is shown as a relative value when the detergency without enzyme (control) is 100%.

  From the results of Tables 5 and 6, it was found that even in the use of an enzyme as a laundry pretreatment agent, a high detergency was exhibited by the combination of a pectin degrading enzyme, manganese peroxidase, and a hydrogen peroxide source.

The cleaning composition of the present invention is used for washing fabrics and clothes, under normal washing conditions using a washing machine, etc., used under conditions such as pre-soaking washing, coating washing, etc. It can be suitably used in a wide range of applications such as use under conditions such as hard surface cleaning.

Claims (3)

  A detergent composition comprising a pectin degrading enzyme, manganese peroxidase, and a hydrogen peroxide source.   The cleaning composition according to claim 1, wherein the hydrogen peroxide source is at least one selected from hydrogen peroxide, a hydrogen peroxide generating compound and a hydrogen peroxide producing enzyme. Furthermore, the cleaning composition in any one of Claim 1 to 2 containing a manganese compound.