JP2006160668A - Lipid peroxide production inhibitor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a lipid peroxide production inhibitor which is safe for human bodies. <P>SOLUTION: Both an Apocynum venetum L (Basikurumon) extract and a vitamin C compound are used to significantly inhibit lipid peroxide production. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

The present invention relates to a lipid peroxide production inhibitor and a lipid peroxide production inhibition method. More specifically, a lipid peroxide production inhibitor characterized by containing Rafu hemp extract and vitamin C, and a method for inhibiting lipid peroxide production comprising using Rafu hemp extract and vitamin C in combination. About.

The state of excessive oxidation of lipids in the living body is considered to trigger liver dysfunction, dementia, arteriosclerosis, diabetes, hypertension and hyperlipidemia.

Also, in skin and hair, if the increase in the amount of lipid peroxide in the skin tissue or secreted over a long period of time, normal skin and hair growth will be hindered, causing phenomena such as dandruff, itching, rough skin, hair loss, and gray hair. Furthermore, it is considered that the skin is also responsible for the occurrence of spots due to melanin deposition.

Therefore, attempts to apply lipid peroxide production inhibitors in the fields of food and drink, cosmetics, and pharmaceuticals have been made for a long time. For example, the flavonoid contains one or more of Morin, Chrysin, Ogonin, Reifolin, Hesperetin, Neohesperidin, Biocanin A, Ferramlin, Chemferol, or one or one of the above flavonoids A lipid peroxide production inhibitor containing a plant extract containing the above is known (for example, see Patent Document 1).

As for Rafu hemp, the ingredients contain hyperoside and isoquercitrin (for example, Non-Patent Document 1), and the hyperoside, which is an ingredient of Rafu, has a strong lipid peroxidation inhibitory effect. (For example, Non-Patent Document 2) is known that roasted rabu hemp leaf extract suppresses oxidation of LDL by copper ions (for example, Non-Patent Document 3).

Japanese Unexamined Patent Publication No. 03-5423 Biopharmaceutical journal, 47 (1), 27-33 (1993) Natural Medicines, 48 (4), 322-323 (1994) Japanese and Chinese Journal of Pharmaceutical Sciences, 13,306-307 (1996)

An object of the present invention is to provide a safe lipid peroxide production inhibitor. Furthermore, it aims at providing the production | generation suppression method of the lipid peroxide in the living body which is onset factors, such as arteriosclerosis.

As a result of intensive studies to solve the above-mentioned problems, the present inventors have confirmed that the formation of lipid peroxide is synergistically suppressed by using Rafu hemp extract and vitamin C in combination. I found it. The present invention has been completed based on such findings.

That is, the present invention is as follows:
Item 1. A lipid peroxide production inhibitor comprising a rafu extract and vitamin C.
Item 2. A method for inhibiting lipid peroxide production, comprising using Rafu hemp extract and vitamin C in combination.
Item 3. A food or drink containing an effective amount of the lipid peroxide production inhibitor according to claim 1.
Hereinafter, the present invention will be described in detail.

The lipid peroxide production inhibitor of the present invention is characterized by containing Rafu hemp extract and vitamin Cs.

Here, Rafu hemp (scientific name: Apocynum venetum L) is a perennial lodging plant belonging to the oleander family. It is also called Bashi-kurumon, Red Linen, or Sawa Urushi Line, and the ones with tea leaves are called Oolong Tea or Okicha. Rafu hemp may be the whole plant body or a part of the plant body such as leaves, flowers, stems, seeds and roots, but is preferably a part containing leaves or leaves.

The rabu hemp (preferably leaves) used for the extraction may be any of freshly collected, fermented or semi-fermented, dried or roasted, but preferably Hemp leaves are dried.

The solvent used for the extraction of Rafu hemp is not particularly limited and may be water, polar organic solvent or nonpolar organic solvent, but preferably water, polar organic solvent or a mixture thereof (hydrous Polar organic solvent). Here, as the polar organic solvent, lower alcohols having 1 to 6 carbon atoms, preferably 1 to 3 carbon atoms such as methanol, ethanol, propanol, isopropanol and butanol; polyhydric alcohols such as glycerin, propylene glycol and polyethylene glycol; or Acetone, ethyl acetate, methyl acetate and the like can be exemplified. The extraction solvent is preferably water, a lower alcohol and a mixture thereof (hydrous alcohol), more preferably water, ethanol and a mixture thereof (hydrous ethanol). In addition, the content ratio of alcohol (ethanol) in the solution in the case of using hydrous alcohol, particularly hydrous ethanol is not limited, but is preferably 10 to 90% by volume, preferably 10 to 60% by volume, more preferably Can exemplify a range of 20 to 40% by volume.

As the extraction method, generally used methods can be widely adopted. Although not limited, for example, a method of immersing the above-mentioned Rafu hemp in an extraction solvent (immersion method) or a method of adding Rafu hemp into the extraction solvent and refluxing while heating (heating reflux method) can be exemplified. . In addition, in the case of the immersion method, any of heating (heating, high temperature), room temperature or cooling (low temperature) conditions may be used, either immersion in a stationary state or immersion with stirring. Good.

The extract obtained by such extraction operation is subjected to various solid-liquid separation means, and a residue (insoluble solid content) insoluble in the solvent is removed. Here, various solid-liquid separation means such as decantation, filtration, centrifugal separation or pressing can be used as the solid-liquid separation means. The extract (filtrate, supernatant, compressed solution) thus obtained can be used as it is, or further diluted with a polar organic solvent such as water, ethanol or a mixture thereof. Also, the extraction solvent is distilled off and partially concentrated or dried (including reduced-pressure drying, freeze-drying, spray drying, etc.) to give a paste (or extract viscous product) or powder (or extract dried product) Can also be used. Further, after the extract is concentrated or dried, the concentrate or the dried product may be further purified by washing with an insoluble solvent, or may be used with a suitable solvent (preferably polar such as water or ethanol). It may be used by dissolving or suspending in an organic solvent or a mixture thereof.

In addition, the extract may be subjected to purification treatment for the purpose of deodorization or decoloration after being concentrated or dried as necessary. Such a purification method is not particularly limited and can be carried out by arbitrarily combining conventional purification methods. Specifically, various resin treatment methods (adsorption method, ion exchange method, gel filtration method, etc.), Examples include supercritical extraction methods, membrane treatment methods (ultrafiltration membrane treatment methods, reverse osmosis membrane treatment methods, ion exchange membrane treatment methods, etc.), solvent fractionation methods, activated carbon treatment methods, and the like.

As for the rabu hemp extract, if it contains the rabu hemp extract described above, preferably the rabu hemp leaf extract (regardless of the extract itself, its concentrate, dried solid or refined product). It may be composed only of these extracts, but may contain a diluent, carrier or other additive as a component other than the extract.

Vitamin C is L-ascorbic acid or a salt thereof, or a derivative of L-ascorbic acid, such as sodium L-ascorbate, L-ascorbic acid monostearate, L-ascorbic acid monopalmitate, L-ascorbic acid monooleate. Ascorbic acid monoalkyl or monoalkenyl esters such as L-ascorbic acid monophosphate, L-ascorbic acid-2-sulfate, etc., ascorbic acid monoester derivatives, L-ascorbic acid distearate, L-ascorbic acid dipalmitate, L -L-ascorbic acid dialkyl or dialkenyl ester derivatives such as ascorbic acid dioleate, L-ascorbic acid tristearate, L-ascorbic acid tripalmitate, L-ascorbic acid trioleate And L-ascorbic acid triester derivatives such as L-ascorbic acid trialkyl or trialkenyl ester derivatives, L-ascorbic acid triphosphate, and the like. Among these L-ascorbic acid or a salt thereof or a derivative of L-ascorbic acid, particularly preferred are L-ascorbic acid, L-ascorbic acid phosphate, and salts thereof.

The amount of use of the rabu hemp extract and vitamin C required for the suppression of lipid peroxide production of the present invention is such that the addition amount of the rabu hemp extract is 0.001 to 100 parts by weight, preferably 0.01 to 100 parts by weight. 20 parts by weight and the amount of vitamin C added is 0.0001 to 10 parts by weight, preferably 0.001 to 1 part by weight.

The lipid peroxide production inhibitor of the present invention only needs to contain the above-mentioned Rafu hemp extract and vitamin C, and may consist only of Rafu hemp extract and vitamin C. As well as diluents, carriers or other additives.

The diluent or carrier is not particularly limited as long as it does not interfere with the effects of the present invention. For example, sugars such as sucrose, glucose, dextrin, starches, cyclodextrins, trehalose, lactose, maltose, starch syrup, and liquid sugar Alcohols such as ethanol, propylene glycol and glycerin; sugar alcohols such as sorbitol, mannitol, xylitol, erythritol and maltitol; polysaccharides such as gum arabic, xanthan gum, carrageenan, guar gum and gellan gum; and water. Examples of the additive include antioxidants, auxiliaries such as chelating agents, fragrances, spice extracts, preservatives, and the like.

For the convenience of use, when preparing a lipid peroxide production inhibitor using these diluents, carriers, or additives, Rafu hemp extract (converted as a dry solid) suppresses lipid peroxide production. A ratio of 0.01 to 50% by weight, preferably 0.1 to 30% by weight, and 0.001 to 10% by weight, preferably 0.01 to 5% by weight, of vitamin C in 100% by weight of the agent It is desirable to prepare such that

In addition, as an antioxidant used as an additive here, what is used as a food additive can be illustrated widely. For example, but not limited to, erythorbic acids such as erythorbic acid and its salts (eg sodium erythorbate); sulfites such as sodium sulfite, sodium hyposulfite, sodium pyrosulfite or potassium pyrosulfite; α-tocopherol, mixed tocopherol, etc. Tocopherols; Dibutylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), etc .; Ethylenediaminetetraacetic acids such as disodium calcium ethylenediaminetetraacetate and disodium ethylenediaminetetraacetate; Gallic acids such as gallic acid and propyl gallate; Extract, Aspergillus terreus extract, Licorice oily extract, Edible canna extract, Globe extract, Essential oil removal fennel extract, Horseradish extract, Sage extract, Seri Extract, tea extract, tempeh extract, dokudami extract, fresh coffee bean extract, sunflower seed extract, pimenta extract, grape seed extract, blueberry leaf extract, propolis extract, hego ginkgo biloba extract, Pepper extract, spinach extract, bayberry extract, eucalyptus leaf extract, gentian root extract, rutin (extract) (red bean whole plant, enju, buckwheat whole plant extract), rosemary extract, clove extract, Extracts from various plants such as apple extract; enzyme-treated rutin, quercetin, rutin enzyme-degraded product (isoquercitrin), enzyme-treated isoquercitrin, enzyme-decomposed apple extract, sesame oil extract, rapeseed oil extract, rice bran Examples thereof include an oil extract, a rice bran enzyme degradation product, gallic acid and esters thereof. Preferably, plant extracts such as bayberry extract, rutin (extract), fresh coffee bean extract, rosemary extract; enzyme-treated rutin, rutin enzyme degradation product (isoquercitrin), enzyme-treated isoquercitrin, etc. Can be mentioned.

Particularly preferred antioxidants in the present invention include flavonols. Examples of the flavonols include flavonols and flavonol glycosides having flavonols in the aglycon part. For example, what can be obtained by extracting from plants such as Enju, tartary buckwheat, dokudami and bayberry, further purified products obtained by purifying it, and those subjected to various treatments such as enzyme treatment or hydrolysis. it can. Specific examples include rutin, isoquercitrin, gospitullin, myricitrin, and the like, as well as glycosyltransferase rutin obtained by treatment by a known method using a glycosyltransferase in the presence of rutin, isoquercitrin and starch. Can be illustrated. These flavonols may be used alone or in any combination of two or more.

Moreover, it can replace with flavonol itself and can also use the plant extract containing various flavonols hung up above as it is. Examples of such include an enju extract, a tartary buckwheat extract, a dokudami extract, and a bayberry extract. In addition, such a plant extract can be obtained by extracting a corresponding part of a plant containing a relatively large amount of flavonols with water, alcohol or other organic solvent. It can also be used after enzymatic treatment. For example, a bayberry extract can be prepared and obtained by extracting a bayberry plant using the method of patent application by the present applicant (JP-A-5-156249, JP-A-9-87619). The bayberry extract obtained is a source of myricitrin (myricetin-3-O-rhamnoside), which is a flavonol glycoside. The bayberry extract can be used as it is, but can also be subjected to a glycosyltransferase treatment according to the method described in JP-A-9-95672, which is used as a readily water-soluble bayberry extract. Can do. In addition, even if these plant extracts are used individually by 1 type, 2 or more types can also be used in arbitrary combinations.

In addition, since some of the flavonols mentioned above are poorly water-soluble and difficult to handle, flavonols may be dissolved in a lower alcohol such as ethanol or a polyhydric alcohol such as glycerin or propylene glycol as necessary. .

When using an antioxidant, the proportion of the antioxidant blended in 100% by weight of the lipid peroxide production inhibitor is not limited. For example, when using enzyme-treated isoquercitrin, 0.01 to 50 % By weight, preferably 0.1 to 30% by weight. Other antioxidants can be used in accordance with this.

The form of the lipid peroxide production inhibitor of the present invention is not particularly limited, and is, for example, a solid state such as a powder, granule, or tablet; a solution such as a liquid or emulsion; or a semi-solid such as a paste It can be prepared in any form such as a shape.

The lipid peroxide production inhibitor of the present invention can be widely applied to various products, and examples thereof include foods and drinks, cosmetics, pharmaceuticals, quasi drugs, and feeds.

Examples of foods and drinks targeted by the present invention include milk drinks, lactic acid bacteria drinks, soft drinks with fruit juice, soft drinks, carbonated drinks, fruit drinks, vegetable drinks, vegetable / fruit drinks, alcoholic drinks, powdered drinks, coffee drinks, tea Beverages such as beverages, green tea beverages and barley tea beverages; custard pudding, milk pudding, souffle pudding, puddings including fruit juice pudding, desserts such as jelly, bavaroa and yogurt; ice cream, ice milk, lacto ice, milk ice cream , Frozen ice cream and soft ice cream with ice cream, ice candy, sherbet, ice confectionery, etc .; gums such as chewing gum and bubble gum (plate gum, sugar-coated grain gum); in addition to coated chocolate such as marble chocolate, strawberry chocolate, Blueberry chocolate and melon chocolate Chocolates such as chocolate with a flavor such as rate; hard candy (including bonbon, butterball, marble, etc.), soft candy (including caramel, nougat, gummy candy, marshmallow, etc.), caramels such as drop, toffee Baked confectionery such as hard biscuits, cookies, rice crackers, rice crackers (and so on); soups such as consomme soup and potage soup; pickled in soy sauce, pickled in soy sauce, pickled in salt, miso pickled, pickled in pickles, pickled in pickles, pickled in pickles , Pickled cucumber, pickled moromi, pickled plum, pickled vegetables such as pickled pickles, shiba pickled, ginger pickled, pickled ume vinegar; sauces such as separate dressing, non-oil dressing, ketchup, sauce, sauce; strawberry jam, blueberry jam, marmalade, apple Jam, apricot jam, preza Jams such as red wine; fruit wines such as red wine; processed fruits such as syruped cherry, apricots, apples, strawberries, peaches; processed meat products such as ham, sausage, grilled pork; fish ham, fish sausage, fish meat surimi, Fish paste products such as salmon, bamboo rings, hampen, fried Satsuma, Date roll, whale bacon; dairy and fat products such as butter, margarine, cheese, whipped cream; udon, cold wheat, somen, buckwheat, Chinese soba, spaghetti, macaroni, Examples include noodles such as rice noodles, rice bran and wonton; various other prepared foods and various processed foods such as rice cakes and rice cakes. Beverages and confectionery are preferred.

The food and drink of the present invention are produced according to conventional production methods for various foods and drinks except that the rafu extract and vitamin C or the lipid peroxide production inhibitor of the present invention is blended in any step of production. can do. There is no particular limitation on the blending method and the order of the Rafu hemp extract and vitamin C, or lipid peroxide production inhibitor.

Cosmetics targeted by the present invention include skin cosmetics (lotions, emulsions, creams, etc.), lipsticks, sunscreen cosmetics, makeup cosmetics, etc .; pharmaceuticals include various tablets, capsules, drinks, troches, gargles Examples of quasi-drugs include toothpastes, mouth fresheners, bad breath prevention agents, etc .; examples of feeds include cat food, dog food and other pet foods, food for ornamental fish and cultured fish, etc. However, it is not limited to these.

Various products such as cosmetics, pharmaceuticals, quasi-drugs, and feeds are excluded except that the rabu hemp extract and vitamin C or the lipid peroxide production inhibitor of the present invention are blended in any process of their production. For example, it can be produced according to conventional methods for various products. There is also no particular limitation on the blending timing of Rafu hemp extract and vitamin C, or lipid peroxide production inhibitor to cosmetics, pharmaceuticals, quasi drugs or feed.

ADVANTAGE OF THE INVENTION According to this invention, the lipid peroxide production suppression suppression method and lipid peroxide production inhibitor which can suppress lipid peroxide production significantly can be provided. For this reason, by applying the method for inhibiting the production of lipid peroxide and the agent for inhibiting the production of lipid peroxide according to the present invention, it is possible to remarkably inhibit the production of lipid peroxide in foods, drinks, cosmetics, pharmaceuticals and the like.

EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited to these Examples. Unless otherwise specified, “parts” means “parts by weight”. Items marked with “*” in the text represent products of Saneigen FFI Co., Ltd., and “*” in the text means a registered trademark of Saneigen FFI Co., Ltd.

Experimental example 1
Crushed hemp leaf dry matter (dry weight 100g) was pulverized, heated and extracted with 8 times the amount of 35% ethanol (75 ° C for 4 hours), filtered, and then synthetic adsorption resin (Separbeads SP-207: Mitsubishi Chemical Corporation) The liquid flow treatment was performed. What added ethanol so that it might become 20% (solid content 4.5%) was prepared, and this was made into the Rafu hemp extract.

Experimental example 2
Add 100 mL of isotonic solution (10 mM phosphate buffer, 152 mM NaCl, pH 7.4) to 100 mL of rabbit stored blood (manufactured by Nihon Biotest), stir well and centrifuge at 1,500 g for 20 minutes to remove the supernatant. To do. This operation was repeated three times, and 100 mL of a hypotonic solution (10 mM phosphate buffer, pH 7.4) was added to the obtained erythrocytes, and after stirring well, the mixture was centrifuged at 20,000 g × 40 minutes to obtain a rabbit as a precipitate fraction. An erythrocyte membrane ghost (hereinafter abbreviated as erythrocyte membrane) was obtained. The erythrocyte membrane was measured using the Lowry method (Lowry, OH, Rosebrough, NJ, Farr, AL, and Randall, RJ, Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951)). The concentration was adjusted to 3 mg protein / mL with a phosphate buffer.

Sample prepared in the stoppered test tube so as to have each final concentration shown in Table 1 below (Rafu hemp extract of Experimental Example 1, L-ascorbic acid (manufactured by Wako Pharmaceutical Co., Ltd.) (hereinafter referred to as AsA)) 100 μL and 850 μL of red blood cell membrane were added and stirred well. Next, 50 μL of 24 mM t-BuOOH aqueous solution was added and incubated at 37 ° C. for 120 minutes to induce oxidation. Immediately after the reaction, the mixture was ice-cooled and the lipid peroxidation degree (TBARS) was determined by the TBA method (Ohkawa, H., Ohishi, N., Yagi, k., Anal. Biochem., 95, p351-358 (1979) Assay for lipid. peroxides in animal tissues by thiobarbituric acid reaction.).

That is, 0.5 ml of the reaction solution after induction of oxidation was collected, and 0.5 ml of 35% TCA (trichloro acetic acid) solution, 1.0 ml of 0.5% TBA solution, 0.2% BHT (butylated) After adding 0.05 ml of hydroxytoluene) solution and 0.05 ml of 0.5% SDS (sodium dodecyl sulfate) solution, the mixture is heated at 100 ° C. for 30 minutes. After cooling, 0.5 ml of acetic acid and 1.0 ml of chloroform were added and stirred, followed by centrifugation (3,000 rpm × 10 minutes), and the absorbance of the upper layer was measured at 532 nm to determine the amount of lipid peroxide. The result was expressed as 100% lipid oxidation degree (TBARS) with no addition (phosphate buffer).

The TBA method is a method suitable for measuring lipid peroxide in a living tissue with high sensitivity, and there are still many reports including a modified version thereof. In the TBA method, a sample is heated with TBA under an acidic condition, and a lipid peroxidation degree is measured by quantifying a red pigment produced by the reaction between TBA reactive substance (TBARS) released from the sample and TBA. . That is, the higher the TBARS value, the more lipid peroxide was generated.

TBARS measured by the TBA method is a reaction product of lipid hydroperoxide, malondialdehyde, aldehyde and protein, and the TBA method comprehensively measures these.

The addition of Rafu extract alone suppressed lipid peroxidation of erythrocyte membranes in a concentration-dependent manner. On the other hand, addition of AsA alone did not suppress oxidation at all, and the amount of TBARS produced did not change from that without addition.

However, when Rafu hemp extract and AsA, which had no effect at the same time, were added at the same time, the lipid peroxidation inhibiting ability of the rafu hemp extract was improved.

In addition of 0.002% Rafu extract (equivalent to 0.044% addition of Rafu hemp extract preparation with a solid content of 4.5%), TBRS was 81.4%. Addition showed synergy up to 62.2%, and lipid peroxidation of the erythrocyte membrane was significantly suppressed (p <0.05).

From these facts, it can be seen that lipid peroxidation of the erythrocyte membrane is more effectively suppressed by simultaneously adding Rafu hemp extract and AsA, and the combination of Rafu hemp extract and AsA is effective for lipid peroxidation. It is suggested.

Example 1
Crushed hemp leaves (100g dry weight) were pulverized, heated and extracted with 8 times the amount of 35% ethanol (75 ° C for 4 hours), filtered, and then a synthetic adsorption resin (Separbeads SP-207: Mitsubishi Chemical Corporation) The liquid flow treatment was performed. This was concentrated to a solid content of 8 to 12%, glycerin 20% and ethanol 20%. This was named Rafu Hemp Extract A.

Example 2 Lemon beverage Fructose glucose liquid sugar 10 (%)
5 times concentrated lemon transparent fruit juice 1
Lemon flavor 0.15
Citric acid 0.09
Trisodium citrate 0.01
Rafu hemp extract A 1
L-ascorbic acid 0.1
Bring the total amount to 100% with water.

  The solution prepared by mixing all the above components is sterilized at 93 ° C. and filled into a 500 ml PET bottle while hot. This was cooled to room temperature to prepare a rabu hemp extract-containing beverage (lemon).

Example 3 Orange Jelly <Raw Material A>
Sugar 5 (%)
Fructose dextrose liquid sugar 10
Gelling agent 0.8
<Raw material B>
5 times concentrated citrus mixed turbid juice 2
Orange fragrance 0.2
Carotene pigment 0.3
Rafu hemp extract A 2
L-ascorbic acid 0.1
Bring the total amount to 100% with water.

All components of <Raw material A> were dispersed in water, heated to 80 ° C. and stirred and dissolved for 10 minutes. After adjusting the pH to 4 with citric acid, <Raw material B> was added and filled into a translucent polyethylene container. . This was sterilized by heating at 85 ° C. for 20 minutes, and then cooled to prepare a rasa extract-containing jelly.

Example 4 Acidic Milk Beverage Fermented milk (non-fat milk solid content 9.5%) 10 (%)
Granulated sugar 7
Stabilizer for acidic milk beverages 0.2
Yogurt flavor 0.13
50% citric acid aqueous solution appropriate amount Rafu hemp extract A 1
L-ascorbic acid 0.1
Bring the total amount to 100% with water.

Fermented milk, granulated sugar and acid milk beverage stabilizer were dissolved in water and then adjusted to pH 3.8 with 50% citric acid solution. This was heated to 70 ° C. in a hot water bath and homogenized (150 kg / cm ² = 14710000 Pa), then heated to 90 ° C., and added to the fragrance, rafu extract 1 and ascorbic acid, and heated to 93 ° C. Hot-packed into a 500 ml PET bottle to prepare an acid milk beverage containing Rafu hemp extract.

Example 5 Milk coffee Granulated sugar 7 (%)
Coffee (coarse) 5
Milk 25
Emulsification stabilizer 0.13
Coffee flavor 0.15
Rafu hemp extract A 3
L-ascorbic acid 0.2
Bring the total amount to 100% with water.

Coffee was added with boiling water and boiled for 40 minutes, followed by filtration to obtain a coffee liquid. An emulsion stabilizer and granulated sugar were added to water, heated to 80 ° C., stirred for 10 minutes, cooled, added with milk, and adjusted to pH 6.8 with a 10% sodium bicarbonate solution. This was combined with the coffee liquor, and Rafu hemp extract 1 and ascorbic acid were further added to adjust the pH to 6.8 again. The mixture was heated to 70 ° C. in a hot water bath and homogenized (150 kg / cm ² = 14710000 Pa). After adding a fragrance | flavor and filling a 200 ml can, it sterilized at 124 degreeC for 20 minute (s), and the rabu hemp extract containing milk coffee was prepared.

According to the method for inhibiting lipid peroxide production of the present invention, it is possible to provide a method for inhibiting lipid peroxide production and a lipid peroxide production inhibitor capable of significantly inhibiting lipid peroxide production. By applying the method for inhibiting lipid peroxide production and the lipid peroxide production inhibitor of the present invention, it is possible to remarkably inhibit the production of lipid peroxide in foods, drinks, cosmetics, pharmaceuticals and the like.

The synergy of Rafu hemp extract and ascorbic acid in inhibiting lipid peroxide production is shown.

Claims (3)

A lipid peroxide production inhibitor comprising Rafu hemp extract and vitamin C. A method for inhibiting lipid peroxide production, comprising using Rafu hemp extract and vitamin C in combination. A food or drink containing an effective amount of the lipid peroxide production inhibitor according to claim 1.

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