JP2006131916A - Antioxidant, and food and cosmetics using it - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant having excellent antioxidant performances such as prevention of deterioration in quality due to oxidation and the like, and food and cosmetics using it. <P>SOLUTION: The antioxidant contains an extract extracted from at least one selected from flesh of a chestnut, the astringent skin of a chestnut, and the outer skin of a chestnut. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  TECHNICAL FIELD The present invention relates to an antioxidant having an excellent antioxidant ability, which contains an extract extracted from chestnut flesh, chestnut astringent skin, and chestnut demon skin, and food and cosmetics using the same.

In general, most living organisms including humans use oxygen, and from various foods, linoleic acid, linolenic acid, arachidonic acid, eicosapentaenoic acid play an important role in maintaining homeostasis and function in the living body. It is said that it maintains life activity by ingesting fatty acids having double bonds such as docosahexaenoic acid.
However, along with this, active oxygen and lipid peroxides are produced in the human body, but these are highly toxic, and recent studies have shown that adult diseases such as carcinogenesis, ischemic disease, diabetes, etc. It is becoming clear that it is deeply involved in cell dysfunction and aging.
In order to protect human tissues from such obstacles, various antioxidants function in the human body, but many of the antioxidants depend on food.

Therefore, in order to prevent such diseases, research on the search for antioxidant substances in foods, elucidation of action, or manufacturing methods has progressed from the viewpoint of controlling active oxygen and lipid peroxide in vivo. It's getting on.
For example, tea leaves and cacao beans are known to contain various antioxidants. In addition, it is known that an extract obtained by extracting chestnut squid and leaves with water or a hydrophilic organic solvent has an anti-inflammatory action, an antihistamine action, and the like (see Patent Documents 1 to 3). However, the effectiveness of chestnut flesh, astringent skin, demon skin, etc., and their antioxidant ability have not been studied.

JP-A-8-217682 JP-A-8-217688 JP-A-8-217690

  The present invention has been made in view of such circumstances, and the object of the present invention is to provide an antioxidant having an excellent antioxidant ability such as prevention of quality deterioration due to oxidation, and foods and cosmetics using the same. It is to provide.

  The above object is achieved by an antioxidant characterized by containing an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

  In addition, the above object is achieved by a food containing an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

  In addition, the above object is achieved by a cosmetic comprising an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

That is, as a result of intensive research to solve the above problems, the present inventors have selected at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin, preferably water, hydrophilic It was confirmed for the first time by experiments that an antioxidant substance having an excellent antioxidant ability can be obtained by extraction using at least one selected from a basic organic solvent and a polyhydric alcohol. An oxidant was found.
Furthermore, the present inventors have found that by containing these antioxidants in foods and cosmetics, they can be made into foods and cosmetics having excellent antioxidant ability such as preventing quality deterioration due to oxidation.

Since the antioxidant of the present invention contains an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin, it has an excellent antioxidant ability. .
In addition, by incorporating the antioxidant of the present invention into foods and cosmetics, it is possible to obtain foods and cosmetics having excellent antioxidant ability such as preventing quality deterioration due to oxidation. In addition, prevention of adult diseases such as cancer, arteriosclerosis, ischemic disease, diabetes, prevention of various diseases caused by the formation of lipid peroxide, prevention of aging, whitening effect, reduction of hangover, recovery from fatigue due to sports, It is expected to be applied to microbial growth inhibition, vitamin / pigment stabilization, deodorizing effect, and the like.
Extracts obtained by extracting at least one selected from chestnut flesh, chestnut astringent skin and chestnut demon skin with an extraction medium and then separating the residue by centrifugation are not only used for food and cosmetics, It can be applied to general industrial products and is versatile.

Next, the present invention will be described in detail.
The antioxidant of the present invention contains an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

  First, the antioxidant of the present invention prevents lipid oxidation, inactivates the oxidation promoting action of metals such as iron and copper, and is more reactive than ordinary oxygen molecules. It is a substance that controls active oxygen and accompanying lipid peroxides.

In general, the seeds from which chestnuts have been removed consist of pulp, chestnut astringent wrapping it, and outermost chestnut demon skin, and the present invention uses extracts extracted from these. Among these, an extract extracted from chestnut astringent skin is particularly preferable in that an antioxidant effect can be obtained. In addition, the antioxidant of this invention may use chestnut flesh, chestnut astringent skin, and chestnut demon skin individually or in combination.
The varieties and sizes of chestnuts used in the present invention are not particularly limited, and may be appropriately selected from those generally used. For example, Japanese chestnuts, European chestnuts, Chinese chestnuts, American chestnuts and the like can be used, and chestnuts subjected to various treatments such as heating, freezing and drying may be used.

  In the antioxidant of the present invention, the content of the extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin is not particularly limited and may be set as appropriate. That's fine.

The antioxidant may contain an auxiliary material appropriately selected within a range not impairing the object of the present invention. Examples of auxiliary materials include saccharide sweeteners (monosaccharides such as fructose, glucose, tagatose and arabinose, oligosaccharides such as lactose, oligosaccharides and maltose, powdered starch syrup, dextrin, trehalose, sugar alcohol, etc.), high sweetness Sweeteners (sucralose, acesulfame, etc.), fats and oils, dairy products, stabilizers, emulsifiers, fragrances, pigments, acidulants, flavor ingredients (eggs, coffee, teas, cocoa, fruit pulp, yogurt, alcoholic beverages), various nutrients (Proteins, dietary fiber, vitamins, minerals, etc.). These may be used alone or in combination.
For example, when dextrin is used as a binder in a chestnut extract, a powdery antioxidant can be obtained.

The form of the antioxidant is not particularly limited, and examples thereof include various forms such as a liquid form, a powder form, a granule form, and a paste form.

The antioxidant of the present invention is produced, for example, as follows.
That is, first, at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin, at least one selected from water, hydrophilic organic solvent, polyhydric alcohol, etc., or a mixture thereof Alternatively, extraction is performed with an aqueous solution or dispersion to obtain an extract. Examples of the extraction method include a method of immersing in an extraction medium for about 5 to 30 minutes and then draining by centrifugation. At this time, it is preferable that the chestnut flesh, chestnut astringent skin, and chestnut demon skin are finely pulverized in order to extract the extract efficiently.
Subsequently, an antioxidant is obtained by adding an auxiliary material to the extract as necessary, and appropriately taking a desired form by a conventional method such as lyophilization or vacuum concentration.

When water is used as the extraction medium in the extraction step, room temperature water or hot water may be used.
Moreover, as a hydrophilic organic solvent, ethanol, methanol, isopropanol, acetone etc. are mentioned, for example.
Examples of the polyhydric alcohol include glycerin, xylitol, maltitol, glucose, xylose, galactose, maltose, sucrose, polyglycerin and the like.
These may be used alone or in combination.

Next, the food of the present invention will be described.
The food of the present invention contains an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

  The food of the present invention may be anything as long as it can contain the above extract, such as chewing gum, candy, tablet, chocolate, frozen dessert, noodles and other starch-based foods, powdered foods, beverages, bread, biscuits, etc. It can be used for any food and drink such as bakery food, soup, fat and oil food, and healthy food and drink. What is necessary is just to select suitably and contain in the step of a manufacturing process according to the characteristic and the objective of each foodstuff.

In addition, the content of the extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin in the food of the present invention varies depending on the type and purpose of each food, Preferably, the content is 0.01% by weight or more based on the total weight of the food. That is, when it is less than 0.01% by weight, the antioxidant effect tends to be difficult to obtain.
The food in the present invention means a food containing an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin, and having antioxidant ability.

  In addition to the extract extracted from at least one selected from the chestnut flesh, chestnut astringent skin, chestnut demon skin, the supplementary ingredients, etc., as long as the original purpose is not impaired. May be appropriately selected and contained.

  As an example of the food of the present invention, candy is produced, for example, as follows. That is, first, as described above, at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin, at least one selected from water, a hydrophilic organic solvent, a polyhydric alcohol, and the like. Extract with one to get the extract. On the other hand, candy raw materials such as granulated sugar and chickenpox are boiled and mixed and cooled. And the said candy raw material mixture will add the said extract and auxiliary material as needed, will further cool, and if it shape | molds suitably, the candy of this invention will be obtained.

Next, the cosmetic of the present invention will be described.
The cosmetic of the present invention contains an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.

Cosmetics may be anything as long as they can contain the above extract, and include, for example, quasi-drugs and bathing agents in addition to skin cosmetics and hair cosmetics.
The form may be appropriately set and used as an arbitrary form such as ointments, lotions, emulsions, creams, packs, gels, powders, granules and the like.

In addition, the content of the extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin in the cosmetics of the present invention varies depending on the type and purpose of each cosmetic, Preferably, it is desirable to set it to 0.01 to 5.0% by weight in the total cosmetic weight.
That is, when it is less than 0.01% by weight, the antioxidant effect tends to be difficult to obtain. On the other hand, even if the amount exceeds 5.0% by weight, the effect corresponding to the blending amount tends to be difficult to obtain.

  In addition, the cosmetics of the present invention are known as cosmetic raw materials such as fats and oils, moisturizers, pigments, dyes, surfactants, ultraviolet absorbers, preservatives, water-soluble polymers, resins and the like as necessary. Any arbitrary component may be appropriately selected and used.

As an example of the cosmetic of the present invention, a skin-beautifying cosmetic is produced, for example, as follows.
That is, first, as described above, at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin, at least one selected from water, a hydrophilic organic solvent, a polyhydric alcohol, and the like. Extract with one to get the extract. And if the said extract is added and mixed in the emulsion prepared according to the conventional method, the skin-care cosmetics of this invention will be obtained.

  Hereinafter, the present invention will be specifically described based on examples.

<Example 1>
<< Preparation of antioxidant >>
First, the chestnut pulp part (10 g), the astringent skin part (4 g) and the demon skin part (10 g) were weighed and then finely pulverized to about 1 mm with a knife to prepare a sample.
Then, 50 ml of distilled water (90 ° C.) is mixed with each ½ amount of each sample, and 50 ml of ethanol (40 ° C.) (reagent special grade manufactured by Wako Pure Chemical Industries, Ltd.) is mixed with each remaining sample. Each was pulverized in a blender at 15000 rpm for 10 minutes.
Next, with respect to the water-soluble fraction, 40 ml of the supernatant was extracted by centrifugation (25000 × g, 20 minutes) and made up to 100 ml with the extraction solvent used. For the ethanol-extracted fraction, the supernatant was extracted by centrifugation (25000 × g, 20 minutes), concentrated to about 1 to 2 ml, diluted to 100 ml with distilled water, and an antioxidant was prepared.

ただし色ブランクがないＢＨＡについては、

とした。
さらに、ＢＨＡについて濃度とラジカル消去能の関係を検量線に表し、これを用いて各試料のラジカル消去能をＢＨＡ濃度に換算して表１に示す。 《Reactive oxygen scavenging ability test》
Active oxygen scavenging ability was measured from the amount of decrease in absorbance accompanying the decrease in DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and the control value.
Extracts of chestnut flesh part, chestnut astringent skin part, chestnut demon skin part obtained in Example 1 above, and food material carrot, pumpkin, spinach extract generally having antioxidant ability as a control sample 2 ml, ethanol 2 ml, and 0.5 mM DPPH / ethanol solution 1 ml were mixed in a test tube, and the absorbance was measured at 517 nm immediately (after 0 minutes) and 30 minutes later. In consideration of the influence of the color of each extract on the measurement results, a system in which ethanol is added instead of the 0.5 mM DPPH / ethanol solution is prepared as a system not involving DPPH radicals. A color blank was used. As a control, 0.1 M acetate buffer was added instead of the extract. Each measurement was performed three times, and an average value was used.
The evaluation of radical scavenging ability was calculated according to the following formula.

However, for BHA without color blanks,

It was.
Furthermore, the relationship between the concentration of BHA and the radical scavenging ability is shown in a calibration curve, and the radical scavenging ability of each sample is converted into the BHA concentration using this, and is shown in Table 1.

《Rodan Iron Method Test》
Antioxidant activity was evaluated by measuring lipid peroxide produced over time by the rodan iron method.
1. Add 1.0 ml of the extract obtained in Example 1 above, 2.5 ml of ethanol containing 9% linoleic acid (99.5%), 0.2 M phosphate buffer (pH 7.0) Take 5 ml of distilled water, 0.5 ml of distilled water, and 0.25 ml of 46.6 mM AAPH (2,2'-azobis 2-amidinopropane dihydrochloride) as a radical generator in a screw-tube test tube. Samples were stored in the vessel protected from light and passed for 15 hours. At this time, instead of the extract obtained in Example 1 as a control, 0.2M phosphate buffer (p
H7.0) was added. In this experiment, 0.1% BHA was used as a control.
After completion of the reaction, 0.1 ml of the reaction solution, 4.7 ml of 75% ethanol, 0.1 ml of 0.02M ferrous chloride / 3.5% hydrochloric acid solution, and 0.1 ml of 30% aqueous ammonium thiocyanate were added for 3 minutes. Thereafter, the absorbance at 500 nm was measured. Each measurement was performed three times, and an average value was used. The antioxidant activity was calculated with the absorbance of the control as 100. Table 2 shows the calculation results.

  Extracts obtained from chestnut flesh, chestnut astringent skin, and chestnut demon skin of Example 1 had antioxidant ability by the active oxygen scavenging ability test and the Rhodan iron method test. In particular, the chestnut astringent skin extract had better antioxidant ability.

<Examples 2 and 3>
<Preparation of candy>
As Example 2, a stamped candy was obtained according to a conventional method. That is, first, 60 g of granulated sugar and 40 g of enzyme starch syrup were mixed as solids, boiled to 150 ° C., and cooled to 130 ° C. Next, 10 g of the chestnut astringent skin water extract obtained in Example 1 and a small amount of citric acid, fragrance and pigment were added to and mixed with the mixture. Got.
Further, as Example 3, a candy was obtained in the same manner as in Example 2 except that 20 g of the chestnut astringent skin water extract obtained in Example 1 was used.
Moreover, the thing which does not use the water extract of the chestnut astringent skin obtained in Example 1 was used as control.
These candy was used as a solution, and the antioxidant activity was evaluated in the same manner as in the above-mentioned Rhodan iron method test. Table 3 shows the calculation results.

  The candy of Examples 2 and 3 has superior antioxidant ability and bitterness compared to a commercially available candy (control) candy not containing an extract extracted from chestnut flesh, chestnut astringent skin, and chestnut demon skin. It was a candy with no inferior taste or coloration.

<Examples 4 to 6, Comparative Example 1>
《Preparation of cosmetics》
A cosmetic (cream) having the composition shown in Table 4 below was produced by a conventional method for producing an emulsion and used as a sample.

[Table 4]
Blending ingredients Blending amount (%)
Example 4 Example 5 Example 6 Comparative Example 1
Stearic acid 7.0 7.0 7.0 7.0
Oleic acid 3.0 3.0 3.0 3.0
Stearyl alcohol 4.0 4.0 4.0 4.0
Glyceryl monostearate 8.0 8.0 8.0 8.0
Ethylparaben 0.1 0.1 0.1 0.1
Butylparaben 0.1 0.1 0.1 0.1
Propylparaben 0.1 0.1 0.1 0.1
Propylene glycol 8.0 8.0 8.0 8.0
Glycerin 2.0 2.0 2.0 2.0
Potassium hydroxide 0.4 0.4 0.4 0.4
Edetate disodium 0.05 0.05 0.05 0.05 0.05
Chestnut pulp water extract obtained in Example 1 3.0 − − −
Chestnut astringent skin water extract obtained in Example 1 -0.1 0.5-
Chestnut demon skin water extract obtained in Example 1 − 0.3 − −
Purified water Residue Residue Residue Residue Residue

A sample of 0.4 g was applied to the cheeks of three panelists twice a day for one week. Thereafter, the effects shown in Table 5 were determined by sensory evaluation.

[Table 5]
sensory evaluation
Irritation Feeling skin effect
Judgment Evaluation Judgment Evaluation I did not feel any irritation ○ The skin became smooth ○
I felt a little irritation △ The skin became slightly smooth △
I felt a sense of stimulation × No change ×

  The sensory evaluation results of Examples 4 to 6 and Comparative Example 1 are shown in Table 6.

[Table 6]
Panel 1 Panel 2 Panel 3
Stimulation Feeling skin effect Stimulation Feeling skin effect Stimulation Feeling skin effect Example 4 ○ △ ○ ○ ○ ○
Example 5 ○ ○ ○ ○ ○ ○
Example 6 ○ ○ ○ ○ ○ ○
Comparative Example 1 ○ × ○ × ○ ×

  As shown in Table 6, it was clarified that Examples 4 to 6 had excellent skin beautifying effects with high safety by sensory evaluation of the items shown in Table 5.

Claims (8)

  An antioxidant comprising an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.   Extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, chestnut demon skin extracted from at least one selected from water, hydrophilic organic solvent, polyhydric alcohol The antioxidant of Claim 1 which is a thing.   A food comprising an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.   4. The food according to claim 3, which has an antioxidant ability.   The food according to claim 3, wherein the extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin contains 0.01% by weight or more based on the total weight of the food. .   A cosmetic comprising an extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin.   The cosmetic according to claim 6, which has an antioxidant ability.   The extract extracted from at least one selected from chestnut flesh, chestnut astringent skin, and chestnut demon skin is contained in an amount of 0.01 to 5.0% by weight based on the total weight of the cosmetic. The cosmetics described.