JP2006094822A - Method for separating microorganism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To precisely separate and recover only microorganisms from a liquid containing the microorganisms, and to measure the microorganisms. <P>SOLUTION: This method for separating microorganisms comprises inserting a mesh filter 2 for recovering the microorganisms into a recovery cell 1 for recovering the microorganisms, disposing an anode side electrode 3 for moving the microorganisms and a cathode side electrode 4 in the upper and bottom portions of the recovery cell 1, respectively, flowing a liquid containing the microorganisms in the recovery cell, applying a direct electric current between the anodic and cathodic electrodes to electrophoretically adhere the microorganisms to the mesh filter 2 disposed in the recovery cell 1, and then separating and recovering only the microorganisms from the liquid containing the microorganisms. The concentration of the microorganisms can be measured. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to the measurement of microorganisms using a method for separating microorganisms using electrophoresis, and more particularly to a method and measurement for efficiently separating only microorganisms from drinking water, foods, and foodstuffs in which microorganisms are mixed. .

Conventionally, this type of microorganism separation method is known as disclosed in JP-A-2000-262865. (See Patent Document 1). Hereinafter, Patent Document 1 will be described with reference to FIG. As shown in FIG. 5, there are two water tanks capable of holding an aqueous solution, one for containing an aqueous solution containing bacteria in advance, and the other for a concentration vessel 102 for concentrating the bacteria and In the meantime, a region 103 capable of migrating bacteria dispersed in an aqueous solution with an electric field is provided, and includes an application means for applying a potential gradient having a strength necessary for migrating the bacteria.
JP 2000-262865 A

  However, with such a conventional method, it is possible to capture and measure the index, which is the concentration of microorganisms in the container, but the number of microorganisms in a liquid such as drinking water or sake is several per milliliter. Therefore, it is required to accurately separate and collect microorganisms from a large amount of liquid.

  The present invention solves such a conventional problem, and when separating microorganisms using charges in a container, a microorganism separation method that can be separated with high accuracy by adding means for collecting microorganisms is provided. The purpose is to provide.

  Moreover, when microorganisms and particles coexist in the cell, there is a problem that the microorganisms cannot be constrained by the shape of the particles, and it is required to separate large-sized particles and microorganisms in advance.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism separation method for separating microorganisms by providing a collection means for collecting large particles when flowing a liquid. And

  In addition, as a collecting means for collecting large particles, it can be separated using weight, specific gravity, electric charge, etc., but depending on the means, microorganisms may be killed due to microbial cell membrane breakage or cessation of activity, and living microorganisms Therefore, there is a need for a method of sending the cells to the cell as they are in the life or death state of the microorganisms.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism separation method using a filter for pores through which microorganisms pass, utilizing the size of microorganisms.

  In addition, although the size of the microorganism is as small as 0.2 to 2 μm, it is larger than the size of the microorganism itself when the size of the microorganism is separated or when the microorganism is aggregated. A filter is requested.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism separation method using a filter having a pore of 7 μm or more in order to increase the passage rate of microorganisms through the filter.

  In addition, separation by electrophoresis polarizes and separates ions and nanoparticles in the liquid, and at the time of recovery, there is a problem that the activity of microorganisms is reduced by the remaining ions and nanoparticles. It is required not to change the characteristics.

  The present invention solves such a conventional problem, and provides a microorganism separation method in which only microorganisms are collected by passing ions and nanoparticles in a liquid, and the characteristics of substances in the liquid are not changed. With the goal.

  In addition, decomposition with the charge of microorganisms has a problem that fine particles having the same mass and charge amount are also collected, and it is required to collect only microorganisms.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism separation method that allows ions and nanoparticles in a liquid to pass through and collects only microorganisms.

  Further, there is a problem that once the microorganisms once separated are deenergized, they are detached at the time of recovery, and it is required to prevent the microorganisms from being detached.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism separation method that prevents detachment of microorganisms during recovery.

  In addition, when the electrode surfaces are provided in parallel, there is a problem in that a current flows locally on the electrode surface to cause convection of the liquid, and a method for suppressing the convection of the liquid is required.

  The present invention solves such conventional problems, prevents uneven current flow, makes one electrode area of a bipolar electrode smaller than the other electrode area, and current flows locally. An object of the present invention is to provide a microorganism separation method that prevents this.

  In addition, since the charge amount of microorganisms is weak, there is a problem that it takes time to recover when the ion concentration in the liquid is high, and it is required that it can be recovered in a short time.

  An object of the present invention is to solve such a conventional problem, and an object of the present invention is to provide a microorganism separation that can be collected in a short time by performing staining to increase the charge of microorganisms.

  Further, there is a problem that the collected microorganisms cannot be efficiently measured in the liquid, and it is required to efficiently measure the microorganisms collected in the liquid.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism measuring apparatus that collects stained microorganisms in a filter and accurately measures the microorganisms by irradiation with light.

  In addition, there is a problem that the staining reagent generates autofluorescence and luminescence and color development, eliminating the difference between the background and the microorganism, and the staining reagent can prevent autofluorescence and luminescence and color development. It is requested.

  The present invention solves such a conventional problem, and provides a microorganism measuring apparatus that prevents autofluorescence, luminescence, and coloration of reagents by staining microorganisms after collection and distinguishes them from the background. Objective.

  Further, there is a problem that the brightness of the collected microorganisms is not different depending on the background brightness, and it is required to prevent the influence of the background brightness.

  The present invention solves such a conventional problem, and an object of the present invention is to provide a microorganism measuring apparatus that reduces the luminance of the background.

  In addition, there is a problem that scattered light is generated by the specimen, increasing the luminance of the background and eliminating the difference in luminance from the microorganism, and further, it is required to prevent the influence of the luminance of the background.

  The present invention solves such a conventional problem, and an object thereof is to provide a microorganism measuring apparatus that prevents generation of scattered light by a specimen and further reduces background luminance.

  In order to achieve the above object, according to the present invention, a collecting means for collecting microorganisms is provided between a flow path for flowing in liquid and a flow path for discharging liquid.

  Thereby, it is possible to obtain high recovery performance by moving in one direction with the microorganism having electric charge in the flow path and recovering the microorganism by the recovery means.

  Further, the microorganism separation method of the present invention is separated from large particles when flowing a liquid containing microorganisms and particles other than microorganisms in order to achieve the above object.

  As a result, microorganisms are restrained and separated from liquids such as foods and beverages with high turbidity, and microorganisms can be separated from various liquids and replaced.

  Further, the microorganism separation method of the present invention is a filter in which pores are formed in order to achieve the above object.

  As a result, the microorganisms in the liquid are restrained and separated while maintaining the state of the microorganisms, and the grown microorganisms in the liquid can be accurately separated.

  In order to achieve the above object, the microorganism separation method of the present invention is such that the diameter of the filter in which the pores are formed is 7 μm or more.

  As a result, microorganisms in the liquid are restrained, and various microorganisms in the liquid can be separated.

  In order to achieve the above object, the microorganism separation method of the present invention uses a filter having micropores as a means for collecting.

  As a result, ions and particles can pass through and only microorganisms can be recovered.

  Further, the microorganism separation method of the present invention is limited to filters having fine pores in order to achieve the above-mentioned object.

  As a result, the particles can pass through and only the microorganisms can be recovered in a short time.

  Further, the microorganism separation method of the present invention is obtained by subjecting a filter having fine pores to a hydrophobic treatment in order to achieve the above object.

  As a result, the protein on the surface of the microorganism is adsorbed, and only the microorganism can be reliably recovered.

  Moreover, in order to achieve the said objective, the microorganism separation method of this invention provides the electrode from which an area differs in the flow path of a cell.

  As a result, the liquid is polarized by electrolysis, a uniform current flow is formed and moved, and can be easily recovered by the recovery means.

  Moreover, in order to achieve the above-mentioned object, the microorganism separation method of the present invention is obtained by staining an inflowing liquid in advance.

  As a result, only the microorganisms are stained, and the collected microorganisms can be easily identified.

  In order to achieve the above object, the microorganism identification apparatus of the present invention directly measures microorganisms decomposed by using charge transfer on a filter in which micropores are formed.

  Thereby, microorganisms can be measured directly from the filter in which the micropores are formed, and can be measured efficiently.

  Further, in order to achieve the above object, the microorganism separation method of the present invention is obtained by staining and measuring microorganisms in a liquid after collecting them in a microporous filter.

  Thereby, the self-fluorescence and color development of the staining reagent in the liquid and the staining reagent remaining on the microporous filter can be reduced, and the stained microorganism can be reliably measured.

  In order to achieve the above object, the microorganism separation method of the present invention is a treatment for absorbing light and color to detect a reaction product produced after staining a microorganism on the filter surface in which micropores are formed.

  As a result, it is possible to confirm a reaction in which only the microorganisms on the filter in which the micropores are formed are stained, and it is possible to accurately measure the microorganisms.

  In order to achieve the above object, the method for separating microorganisms of the present invention filters metal components that absorb various wavelengths in order to detect reactants that are produced after staining only the microorganisms on the filter in which micropores are formed. It is formed above.

  This allows the filter to absorb the autofluorescence, light emission, and color of various specimens, prevents scattered light from the particles and liquid in the specimen, and allows microorganisms to be measured more accurately.

  ADVANTAGE OF THE INVENTION According to this invention, the microorganisms separation method with the effect that the liquid in which particles other than microorganisms and particles other than microorganisms can isolate | separate only a microorganism can be provided.

  According to the present invention, only microparticles equivalent to microorganisms can flow from a turbid liquid, prevent a decrease in flow rate or clogging of microparticles, and have an effect of extending the life of consumables such as cell replacement. Separation methods can be provided.

  ADVANTAGE OF THE INVENTION According to this invention, it can collect | recover while maintaining microbial performance from the liquid, and can provide the microorganisms separation method with the effect that it can isolate | separate stably.

  ADVANTAGE OF THE INVENTION According to this invention, microorganisms can be efficiently isolate | separated from the liquid and the microorganisms separation method with the effect that a microorganisms can be isolate | separated more stably can be provided.

  According to the present invention, the filter formed with micropores can decompose particles caused by aggregation of ionic substances in the liquid and particles affecting measurement, collect only microorganisms, and use industrial microorganisms. Therefore, it is possible to provide a method for separating microorganisms, which is effective.

  According to the present invention, microorganism separation is effective in that it can be reliably separated from microorganisms by a filter with a more limited pore, only microorganisms contained in the liquid can be highly recovered, and industrial microorganisms can be used. Can provide law.

  According to the present invention, a microbe separation method having an effect that a filter formed with micropores collected after separation can be removed, and a culture solution and necessary microorganisms can be collected, propagated and used in an actual microbe evaluation. Can provide.

  According to the present invention, an unequal current flow can be prevented by electrodes having different areas, convection due to Joule heat can be prevented, microorganisms can be distributed uniformly, and the recovery amount can be increased, so that the liquid can be efficiently recovered from a liquid with less microorganisms. In addition, it is possible to provide a method for separating microorganisms, which is effective in recovering microorganisms and easily using liquid microorganisms with few microorganisms.

  According to the present invention, it is possible to provide a microorganism separation method that is effective in that only microorganisms can be accurately measured by providing a staining means, and that pathogenic bacteria can be recovered by staining specific microorganisms.

  ADVANTAGE OF THE INVENTION According to this invention, the microorganisms separation method with the effect that the microorganisms density | concentration in the inflowed liquid can be measured rapidly and accurately by detecting the microorganisms on a filter can be provided.

  According to the present invention, it is possible to provide an effective microorganism separation method in which the characteristics of microorganisms can be investigated later by performing dyeing after being collected on a filter having micropores, and the work can be easily performed such as culture and growth. .

  ADVANTAGE OF THE INVENTION According to this invention, the microorganism separation method which can be dye | stained easily and can recognize microorganisms with high sensitivity, and anyone can measure microorganisms rapidly with high precision easily can be provided.

  ADVANTAGE OF THE INVENTION According to this invention, the microorganisms separation method which has the effect that it can dye | stain easily, can also recognize microorganisms with high sensitivity, and anyone can measure microorganisms quickly with high precision can be provided.

  The invention described in claim 1 of the present invention is a method for separating microorganisms from a liquid containing microorganisms and particles other than microorganisms, and means for flowing a liquid containing microorganisms and particles other than microorganisms into a cell for separating microorganisms. The microorganism separation method is characterized by having a separation means for separating using the charge of microorganisms in the cell and a recovery means for collecting only the separated microorganisms, and has a negative charge. Only the microorganisms are collected by the collecting means while the microorganisms move to the side of the positively charged electrode. As the collecting means, for example, a filter or glass having a hydrophobic hole is used. In addition, positively charged particles other than microorganisms are moved and discharged to the negatively charged electrode side. Further, the negatively charged particles are separated from the microorganisms by the collecting means, reach the positively charged electrode side, and are discharged. Thereby, it has the effect | action of isolate | separating into the liquid without a microorganism.

  Further, in the means for flowing a liquid containing microorganisms and particles other than microorganisms into the cell, the microorganism separation method is characterized by comprising a collecting means for collecting large particles. The shape of the path and the cell can be reduced, and it can be effectively separated in a miniaturized shape.

  Further, as a collecting means for collecting the excessive particles, it is a microorganism separation method characterized in that a filter having a hole is formed, and it is possible to use a microorganism of the recovered microorganism. Has an effect.

  Further, the present invention is a microorganism separation method characterized in that the pore diameter of the filter in which the pores are formed is a diameter of 7 μm or more through which microorganisms pass, and the amount of microorganisms to be recovered is increased to reduce microorganisms in the liquid. It has the effect | action that it can utilize efficiently.

  In addition, as a recovery means for recovering only the separated microorganisms, the microorganism separation method is characterized in that a filter having micropores is formed. It has the effect | action that it can isolate | separate.

  Further, the microbe separation method is characterized in that the pore diameter of the filter having the micropores is 0.2 μm or more through which microorganisms pass, and the microorganisms return to the liquid in which the microorganisms are collected. It has the effect | action that it can prevent and can isolate | separate accurately.

  In addition, the microbe separation method is characterized in that the surface of the filter in which the micropores are formed is subjected to a hydrophobic treatment, and the surface of the microbe and the filter has a stronger bond than the electrical bond, and the filter is removed from the cell. It has the effect that it can be taken out.

  In addition, as a means for separating the cells using the charge of microorganisms in the cell, a bipolar electrode is provided in the cell flow path, but the area of one electrode is set to the other electrode in order to form a uniform current flow. This is a microbe separation method characterized by making the area smaller than the area of the filter, reducing local current generation, preventing convection due to heat, and evenly collecting and detecting on the surface of the collected filter It has an effect that it can be easily performed.

  Further, it is a microorganism separation method characterized by having a staining means for staining a liquid containing microorganisms and particles other than microorganisms, and has the effect that detection sensitivity can be enhanced by staining only microorganisms. Have.

  Further, the present invention is a microorganism measuring apparatus characterized by comprising means for detecting microorganisms captured in the filter in which the micropores are formed, and has an effect that measurement can be performed with a simple configuration by capturing in the filter. Have.

  In addition, as a means for detecting the microorganism, the microorganism measuring apparatus is characterized in that the microorganism captured in the filter having the micropores is stained, and the capacity of the staining reagent is reduced by staining only the microorganism. And has the effect of measuring with high accuracy.

  The microbe separation method is characterized by absorbing light on the surface of the filter in which the micropores are formed, and can prevent surface reflection with respect to the detection of microorganisms. It has the effect of detecting microorganisms.

  Further, as the treatment for absorbing light, a microorganism separation method characterized in that a thin film containing at least one metal component selected from gold, copper, chromium, platinum, and palladium is formed. It is possible to prevent reflection of light when irradiating excitation light at the time of detection, and to detect microorganisms more accurately.

  Embodiments of the present invention will be described below with reference to the drawings.

Example 1
As shown in FIG. 1, a collection cell 1 for collecting microorganisms is provided with a positive electrode 3 for collecting microorganisms at the top and a negative electrode 4 at the bottom so as to sandwich a mesh filter 2 for collecting microorganisms. is there. The positive electrode 3 and the negative electrode 4 are wired so that direct current electricity is supplied from the power source 5. The liquid containing the microorganisms and particles other than the microorganisms is supplied from the supply flow path by the supply pump and flows into the collection cell 1. The liquid containing the flowed microorganisms and particles other than the microorganisms fills the recovery cell 1. After filling, direct current electricity is supplied from the power source 5 to the positive electrode 3 and the negative electrode 4, and electrophoresis starts for a liquid containing microorganisms and particles other than microorganisms. When electrophoresis starts, negatively charged microorganisms and negatively charged particles move to the positive electrode 3 side, and only the microorganisms are captured by the mesh filter 2 on the way, and other negative The charged particles move to the positive electrode 3 of the recovery cell 1.

  Furthermore, by continuously supplying a liquid containing microorganisms and particles other than microorganisms by the supply pump 7, a liquid not containing microorganisms is discharged from the discharge flow path 8, and further, a mesh filter is discharged from the fine particle discharge flow path. The liquid containing the fine particles having passed through 2 is discharged.

  Thereby, microorganisms can be recovered from a liquid containing microorganisms and particles other than microorganisms.

(Example 2)
When a liquid containing microorganisms and particles other than microorganisms is introduced, there is a phenomenon in which microorganisms are hidden in the particles. As shown in FIG. 2, a filter unit 10 is provided for collecting particles larger than the microorganisms contained in the liquid after the liquid containing the microorganisms and particles other than the microorganisms is introduced. It is possible to prevent clogging of the flow path and increase the separation efficiency from microorganisms.

  Furthermore, it is possible to extend the time for replacing the flow path and maintaining the feed pump 7.

  In addition, although it provided in the flow path as the filter unit 10 which collects a big particle, it cannot be overemphasized that processing in advance is also included.

  Further, as the particle collecting means, a means for separating using the characteristics of particles such as centrifugal treatment, electric perturbation or dielectric perturbation may be used.

(Example 3)
As shown in FIG. 2, a filter having a filter unit 10 and a hole was formed. By using a filter, it is possible to prevent a decrease in the activity of microorganisms and to separate microorganisms in an actual liquid.

  Filter types are PP (Polypropylene), PVC (Polyvinyl Chloride), PC (Polycarbonate), PTFE (Polytetrafluorethylene), PVDF (Polyvinylefluoride), MCE (Mixed PE). Depending on these filters, there is no problem even if a surface treatment that does not adhere to microorganisms or a certain surfactant is used to improve the filtration state before use.

  Moreover, you may add an ionic surfactant so that filtration performance may improve. There are five types of ionic surfactants: 1) fatty acid (anion) fatty acid sodium, fatty acid potassium, alpha sulfo fatty acid ester sodium, 2) linear alkylbenzene sulfone, linear alkylbenzene sulfone Sodium sulfate, alkyl alcohol sulfate sodium, alkyl ether sulfate sodium, 4) alpha olefin, sodium alpha olefin sulfonate, 5) sodium alkyl sulfonate, etc. As the amide-based nonionic surfactant, any reagent that does not cause a chemical reaction, dissolves microorganisms, and does not lyse may be used.

Example 4
As shown in FIG. 3, the passage performance of microorganisms when using a filter is shown.

  As a test bacterium, B. Sabtilis (NBRC3134) was used, and the filter used the filter which made the sulfonic acid as the raw material, and grasped | ascertained the passage performance in the hole of a filter. If the filter pores are 7 μm or less, they are trapped, and the microorganisms passage performance is reduced.

(Example 5)
Since the size of the microorganism is 0.2 to 2 μm, the recovery performance can be further improved by reducing the pore diameter of the mesh filter 2 in the recovery cell 1 shown in FIG.

(Example 6)
In order to completely collect microorganisms, the mesh filter 2 shown in FIG. 2 has a pore diameter of 0.2 μm or more. Microbes can be captured completely.

(Example 7)
The surface of the mesh filter 2 was subjected to hydrophobic treatment, and gold was deposited on the mesh filter 2. When microorganisms adhere to the surface of the mesh filter 2 deposited with gold by electrophoresis, the sulfur component of the protein on the surface of the microorganisms forms a covalent bond, and even if electricity supplied from the power source 5 is stopped, it adheres to the surface of the mesh filter 2. Yes.

  Of course, even if it is removed from the collection cell 1, it is attached to the mesh filter 2, so that it can be used for identifying the collected microorganisms.

(Example 8)
The area of the negative electrode 4 in FIG. 1 was made extremely smaller than that of the positive electrode 3 and was shaped like a needle tip. Due to such a shape, a uniform current flow was formed between the positive electrode 3 and the generation of heat in the liquid, thereby preventing convection. By preventing convection, the substance that moves between the electrodes in the collection cell 1 flows uniformly, and microorganisms can be captured uniformly in the mesh filter 2.

  In addition, the same effect will be produced by providing a plurality of extremely small positive electrode 3.

Example 9
Although not shown in the figure, a means for staining microorganisms was provided before flowing into the collection cell 1. As a reagent for staining microorganisms, 4 ′, 6-diamidino-2-phenylindole dihydrochloride, propidium iodide, 6-carboxyfluorescein diacetate, 4-methylumbelliferyl-β-D- There are galactosides and the like, and there are antibodies that have a fluorescent label that reacts, and those that stain only specific microorganisms with microphages. By staining, only microorganisms can be easily identified.

(Example 10)
It is a block diagram which shows a microorganisms weighing device like FIG.

  This microorganism weighing device is provided with a condensing lens 11 below the collection cell 1 as means for detecting microorganisms. In order to extract the target wavelength from the excitation light emitted from the light source 13, the excitation light spectrally separated by the excitation light spectral filter 14 passes through the prism 15, the optical path is changed, and the excitation light whose optical path has been changed. Is condensed on the surface of the microorganisms collected by the mesh filter 2 of the collection cell 1 through the condenser lens 11. Therefore, the fluorescence of the microorganisms excited by the excitation light passes through the prism 15 again and reaches the light receiving unit 12. The fluorescence that has reached the light receiving unit 12 passes through the fluorescence spectroscopic filter 16 in order to extract only the target fluorescence, reaches the light source conversion element 17 built in the light receiving unit, is converted into a signal, and is recognized by the microorganism judging means 18. .

(Example 11)
Although not shown in the drawing, after removing the mesh filter 2 from the collection cell 1 and staining the microorganisms by the staining method, excitation light spectroscopy is used to extract the target wavelength from the excitation light emitted from the light source 13. The excitation light split by the filter 14 passes through the prism 15 to change the optical path, and the excitation light whose optical path has been changed passes through the condenser lens 11 and is collected in the mesh filter 2 of the recovery cell 1. Focused on the surface. Therefore, the fluorescence of the microorganisms excited by the excitation light passes through the prism 15 again and reaches the light receiving unit 12. The fluorescence that reaches the light receiving unit 12 passes through the fluorescence spectroscopic filter 16 in order to extract only the target fluorescence, reaches the light source conversion element 17 incorporated in the light receiving unit, is converted into a signal, and is recognized by the microorganism judging means 18. Thus, microorganisms can be measured. Since the staining reagent is reacted directly with the mesh filter 2, the reactivity is high and the amount of the staining reagent can be reduced.

(Example 12)
In order to absorb light on the surface of the mesh filter 2, a dark filter was used. In the detection by fluorescence, light emission, and color, since it cannot be detected unless there is a difference in luminance and color from the background, a dark color filter is used in order to cope with fluorescence and light emission.

  Thereby, the brightness | luminance of background becomes clear and a microorganism can be detected.

(Example 13)
When the material portion of the mesh filter 2 is exposed, the captured microorganisms cannot be measured. By coating a thin film of a metal selected from gold, silver, copper, chromium, platinum, palladium and the like on the filter surface by vapor deposition, reflection can be prevented. Gold, in particular, has a low ultraviolet reflectance.

  By separating and recovering only microorganisms in food, cosmetics and drinking water, it is possible to detect with fluorescent reagents, luminescent reagent stains and antibody reagents more accurately than before by removing contaminants other than microorganisms. It can be applied to the inspection of microorganisms in the food field, pharmaceutical field, and chemical product field.

The figure which shows the microorganisms separation apparatus structure of Example 1 of this invention. The figure which shows the microorganisms separation apparatus structure of Example 2 of this invention. Graph showing filter pore diameter passage performance The figure which shows the microorganisms separation apparatus structure of Example 10 of this invention. The figure which shows the structure of the microorganisms separation method of a prior art example

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Recovery cell 2 Mesh filter 3 Positive electrode 4 Negative electrode 5 Power supply 6 Supply flow path 7 Supply pump 8 Discharge flow path 9 Fine particle discharge flow path 10 Filter unit 11 Condensing lens 12 Light receiving part 13 Light source 14 Excitation light spectral filter DESCRIPTION OF SYMBOLS 15 Prism 16 Fluorescence spectral filter 17 Light source conversion element 18 Microorganism judgment means

Claims (13)

In a method for separating microorganisms from a liquid containing microorganisms and particles other than microorganisms, a means for flowing the liquid containing microorganisms and particles other than microorganisms into a cell for separating microorganisms, and the charge of the microorganisms in the cells are used. A separation method for separating microorganisms and a collection means for collecting only the separated microorganisms. 2. The method for separating microorganisms according to claim 1, further comprising collecting means for collecting large particles in the means for flowing a liquid containing microorganisms and particles other than microorganisms into the cell. The microorganism separation method according to claim 1, wherein a filter having a hole is used as a collecting means for collecting the large particles. 4. The method for separating microorganisms according to claim 1, wherein the pores having the pores have a pore diameter of 7 μm or more through which microorganisms pass. The microorganism separation method according to claim 1, 2, 3, or 4, wherein the collecting means for collecting only the separated microorganisms is a filter having micropores. 6. The method for separating microorganisms according to claim 1 or 5, wherein the filter having the fine pores has a pore diameter of 0.2 μm or more through which microorganisms pass. The microorganism separation method according to claim 1, 5 or 6, wherein the surface of the filter in which the micropores are formed is subjected to a hydrophobic treatment. As a means for separating by utilizing the charge of microorganisms in the cell, a bipolar is provided in the cell flow path, but in order to form a uniform current flow, the area of one electrode is larger than that of the other electrode. The method for separating microorganisms according to claim 1, 2, 3, 4, 5, 6 or 7. 9. The method for separating microorganisms according to claim 1, further comprising staining means for staining a liquid containing microorganisms and particles other than microorganisms. 10. The method for separating microorganisms according to claim 1, further comprising means for detecting microorganisms captured by the filter in which the micropores are formed. The microorganism separation according to claim 1, 2, 3, 4, 5, 6, 7, 8 or 9, wherein the microorganism captured as a means for detecting the microorganism is stained on the filter in which the micropores are formed. Law. The microorganism separation according to claim 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, wherein the surface of the filter in which the micropores are formed is treated to absorb light. Law. A thin film containing at least one metal component selected from gold, copper, chromium, platinum, and palladium is formed as the light absorbing treatment. The method for separating microorganisms according to 6, 7, 8, 9, 10, 11 or 12.

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