JP2006058194A - Spectrum decoder - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a spectrum decoder capable of increasing the measurement speed, without lowering the measurement precision. <P>SOLUTION: The spectrum decoder is equipped with a band-pass filter selecting means 101, capable of decoding a specimen encoded, based on the difference of a luminance spectrum distribution and selecting the minimum band-pass filter; a spectrum acquiring means 1117 for acquiring the luminance spectrum distribution of the specimen by the selected band-pass filter; a decoding means 1116 for decoding the specimen from the reference spectrum data of the acquired luminance spectrum distribution and the preliminarily measured luminance spectrum distribution of the specimen; and a reference spectrum data storing means 1101 for preliminarily storing the reference spectrum data. By using a band-pass filter in measurement, corresponding to the specimen selected to perform measurement, the measurement time for the specimen is shortened. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a technique for selecting an optimum band pass filter when discriminating types of substances having different luminance spectrum distributions.

  Gene expression has been studied to discover new therapeutic methods for diseases with various genetic associations in order to clarify gene function.

  In recent years, gene expression analysis techniques using semiconductor nanocrystals have been proposed. A semiconductor nanocrystal has a property of emitting light having a different wavelength depending on the composition and size of the semiconductor nanocrystal when irradiated with excitation light having a specific short wavelength (see, for example, Patent Document 1).

  Utilizing this property, a plurality of semiconductor nanocrystals having different emission wavelengths are attached to plastic beads at an arbitrary ratio and coded with a unique luminance spectrum distribution. Further, a probe for binding to a specific mRNA is attached to each code bead, and the code and the mRNA are made to correspond one-to-one. Therefore, the type of mRNA expressed can be analyzed by observing and decoding the luminance spectrum distribution of beads emitted by irradiating excitation light (see, for example, Patent Document 2).

  As a method of measuring fluorescent substances having different emission wavelengths, there is a fluorescence microscope apparatus that performs measurement using a plurality of bandpass filters (see, for example, Patent Document 3).

  FIG. 11 shows a conventional spectrum decoding apparatus that decodes using the luminance spectrum distribution observed using the conventional fluorescence microscope apparatus and identifies mRNA.

  In FIG. 11, the conventional spectrum decoding apparatus includes a reference spectrum data storage unit 1101, a decoding unit 1116, and a spectrum acquisition unit 1117. The spectrum acquisition unit 1117 includes a system controller 1102, a well plate driving unit 1104, and more specifically. , Focusing means 1106, bandpass filter 1109 a to bandpass filter 1109 h, rotation filter 1110, rotation filter driving means 1111, well plate 1103, objective lens 1105, dichroic mirror 1114, excitation light source 1107, excitation The light source control unit 1108, the CCD camera 1112, the CCD camera control unit 1113, and the image processing unit 1115 are configured.

  The reference spectrum data storage unit 1101 has a luminance spectrum distribution (hereinafter referred to as “specific spectral spectrum distribution”) unique to a plurality of materials arbitrarily selected from materials (hereinafter referred to as “substance group”) prepared in advance so as to be discriminated by the difference in luminance spectral distribution. , Referred to as reference spectrum data) and a code corresponding to the reference spectrum data is stored in advance. Here, the substance group refers to beads prepared in advance so as to be distinguishable, and hereinafter referred to as a bead group. The plurality of substances are beads arbitrarily selected from a group of beads, and the reference spectrum data storage unit 1101 stores reference spectrum data and codes corresponding to the beads in advance.

  The luminance spectrum distribution is uniquely determined by the code. FIG. 12 shows an example of the reference spectrum. FIG. 12 shows reference spectra of four types of beads of code A to code D. The luminance value when the wavelength transmitted through each bandpass filter is measured is plotted for each bandpass filter and connected by a straight line, which is referred to as reference spectrum data of each code. ◯ is code A, Δ is code B, □ is code C, and ● is code D reference spectrum data.

  The system controller 1102 arranges an observation target into which a plurality of substances extracted from all or a part of a plurality of substances (hereinafter referred to as a plurality of subjects) are injected within the imaging range of the CCD camera 1112. The well plate driving means 1104 for moving the well plate 1103 in the X-axis and Y-axis directions is controlled. Here, the plurality of subjects are beads within the observation target, and are arbitrarily selected from a plurality of substances. Further, the system controller 1102 is different from the focusing unit 1106 that moves the objective lens 1105 and focuses the beads injected into the observation target, and the excitation light source control unit 1108 that controls the light amount of the excitation light source 1107 that excites the beads. The rotation filter driving means 1111 controls the rotation filter 1110 in which a plurality of bandpass filters 1109 a to 1109 h having transmission band wavelengths are arranged, and arranges an arbitrary bandpass filter on the observation target and the optical path of the CCD camera 1112. Control is performed to control the CCD camera control means 1113 for controlling the photographing of the CCD camera 1112.

  The dichroic mirror 1114 reflects the excitation light from the excitation light source 1107 toward the observation target and transmits light emitted from the beads by the excitation light.

  The image processing unit 1115 obtains for each bandpass filter using the luminance value of the beads shown in the image taken through the bandpass filter 1109a to the bandpass filter 1109h one by one for each bead to be observed. The brightness spectrum distribution of each bead thus obtained is sent to the decoding means 1116 as measured spectrum data.

  The decoding unit 1116 outputs information on the mRNA specified by decoding to an external device.

  Next, code identification performed in the decoding unit 1116 will be described with reference to FIG. For example, when the measured spectrum data of a certain bead obtained by the image processing means 1115 is shown in FIG. 13, the distribution is the same as the reference spectrum data of the code C shown in FIG. I understand.

At this time, a number of band pass filters capable of discriminating bead groups are prepared. Here, in order to discriminate about 200 types of codes, eight band pass filters 1109a to 1109h (hereinafter referred to as all band pass filters). And all band-pass filters are always used.
Special Table 2002-544488 Special table 2004-500109 gazette JP-A-10-309281 (FIG. 1)

  However, in the conventional configuration, even when there are few types of codes in the observation target, an image that has passed through the entire bandpass filter is required for decoding. Therefore, there is a problem that it takes time to capture images as many as the number of bandpass filters.

  The present invention solves the above-described problems, and provides a spectrum decoding device that can reduce the number of times of photographing by reducing the number of band-pass filters used for photographing without degrading the decoding accuracy and increase the measurement speed. For the purpose.

  In order to solve the conventional problem, the spectrum decoding device of the present invention measures the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, A spectrum decoding device for discriminating the type of the substance, wherein a plurality of substances arbitrarily selected from the substances (hereinafter referred to as substance groups) prepared in advance so as to be discriminated by a difference in luminance spectrum distribution Reference spectrum data storage means for storing in advance a unique luminance spectrum distribution (hereinafter referred to as reference spectrum data) and a code corresponding to the reference spectrum data, and taking out the reference spectrum data from the reference spectrum data storage means, To identify the substance group The minimum number of band-pass filters (hereinafter referred to as first band-pass filters) required for discriminating the plurality of substances from the plurality of band-pass filters (hereinafter referred to as all band-pass filters) prepared in the number required for Band pass filter selection means for selecting a group) and a luminance spectrum distribution of a plurality of substances extracted from all or part of the plurality of substances (hereinafter referred to as a plurality of subjects). Spectrum acquisition means acquired using a pass filter group, and decoding means for comparing the luminance spectrum distribution acquired by the spectrum acquisition means with the reference spectrum data to determine the plurality of objects. It is what.

  Furthermore, in the spectrum decoding device, the spectrum decoding device is configured to measure the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and to determine the type of the substance. In addition, a plurality of substances arbitrarily selected from the previously prepared substances (hereinafter referred to as substance groups) so that they can be discriminated by the difference in luminance spectrum distribution, each of which has a unique luminance spectrum distribution (hereinafter referred to as reference spectrum data). And a reference spectrum data storage means for storing a code corresponding to the reference spectrum data in advance, and a plurality of substances (hereinafter referred to as a plurality of subjects) from which all or part of the plurality of substances are extracted. Multiple codes (hereinafter referred to as code groups) corresponding to Subject information storage means for storing, and the reference spectrum data corresponding to the plurality of subjects based on the code group stored in the subject information storage means is extracted from the reference spectrum data storage means, From the plurality of band-pass filters (hereinafter referred to as all band-pass filters) prepared for the number of substances necessary to determine the substance group, the minimum band-pass filters (to be referred to as all band-pass filters) ( Hereinafter referred to as a second bandpass filter group), a spectrum acquisition means for acquiring luminance spectrum distributions of the plurality of subjects using the second bandpass filter group, and the spectrum The luminance spectrum distribution acquired by the acquisition means is compared with the reference spectrum data before It is characterized in that it comprises a decoding means for determining a plurality of subjects, the.

  Furthermore, in the spectrum decoding device, the spectrum decoding device is configured to measure the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and to determine the type of the substance. In addition, a plurality of substances arbitrarily selected from the previously prepared substances (hereinafter referred to as substance groups) so that they can be discriminated by the difference in luminance spectrum distribution, each of which has a unique luminance spectrum distribution (hereinafter referred to as reference spectrum data). And a reference spectrum data storage means for storing a code corresponding to the reference spectrum data in advance, and a plurality of substances (hereinafter referred to as a plurality of subjects) from which all or part of the plurality of substances are extracted. Multiple codes (hereinafter referred to as code groups) corresponding to Subject information storage means for storing, and the reference spectrum data corresponding to the plurality of subjects based on the code group stored in the subject information storage means is extracted from the reference spectrum data storage means, From the plurality of band-pass filters (hereinafter referred to as all band-pass filters) prepared for the number of substances necessary to determine the substance group, the minimum band-pass filters (to be referred to as all band-pass filters) ( The bandpass filter selecting means for selecting the second bandpass filter group) and the codes of the plurality of objects of the observation object that have been discriminated among the observation objects into which the plurality of objects have been injected Group and the second band pass filter group for discriminating the code group (hereinafter referred to as a discriminated second band pass filter group) Based on the discriminated subject information storage means to be stored and the code group output from the subject information storage means, the second bandpass filter group output by the bandpass filter selection means or the discriminated subject information A selector that selects one of the discriminated second bandpass filter groups output by the storage means and outputs the selected group as a third bandpass filter group, and a luminance spectrum of the plurality of subjects using the third bandpass filter group A spectrum acquisition unit that acquires a distribution; and a decoding unit that compares the luminance spectrum distribution acquired by the spectrum acquisition unit with the reference spectrum data to determine the plurality of subjects, and the selector includes the subject The code group output from the information storage means and the code group stored in the discriminated subject information storage means are one. In the case of matching, the discriminated second bandpass filter group is set as the third bandpass filter group, the code group output from the subject information storage means and the code group stored in the discriminated subject information storage means If the two do not match, the band pass filter selection means is instructed to select the second band pass filter group, and the second band pass filter group is set as the third band pass filter group. Spectral decoding device.

  Furthermore, in the spectrum decoding device, the spectrum decoding device is configured to measure the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and to determine the type of the substance. In addition, a plurality of substances arbitrarily selected from the previously prepared substances (hereinafter referred to as substance groups) so that they can be discriminated by the difference in luminance spectrum distribution, each of which has a unique luminance spectrum distribution (hereinafter referred to as reference spectrum data). And a reference spectrum data storage means for storing a code corresponding to the reference spectrum data in advance, and taking out the reference spectrum data from the reference spectrum data storage means and determining the substance group Number of sheets prepared In order to discriminate a plurality of substances (hereinafter referred to as a plurality of analytes) obtained by extracting all or part of the plurality of substances from the plurality of bandpass filters (hereinafter referred to as an all bandpass filter). Bandpass filter selection means for selecting a minimum bandpass filter (hereinafter referred to as a fourth bandpass filter group), and luminance spectrum distributions of the plurality of subjects using the fourth bandpass filter group. Spectrum acquisition means to acquire, luminance spectrum distribution acquired by the spectrum acquisition means to compare with the reference spectrum data to discriminate the plurality of subjects, and code groups of the plurality of subjects to the bandpass filter selection means The bandpass filter selection means is an observation object into which the plurality of subjects have been injected. In the case where there are a plurality of the plurality of observation objects, when acquiring the luminance spectrum distributions of the plurality of subjects in the first observation object, the spectrum is determined by using the all band-pass filters as the fourth band-pass filter group. The fourth band pass filter group is output based on the code group output by the decoding means when acquiring luminance spectrum distributions of the plurality of subjects in the second and subsequent observation objects. It selects and outputs to the said spectrum acquisition means.

  Furthermore, in the spectrum decoding apparatus, the plurality of subjects in the plurality of observation objects all include the same type of code group.

  Further, in the spectrum decoding apparatus, the bandpass filter selection means can discriminate between the plurality of substances or the plurality of analytes (hereinafter referred to as input substances) of the input code (hereinafter referred to as input code group). And a minimum bandpass filter selecting means for selecting one or a plurality of bandpass filter group candidates composed of a minimum number of bandpass filters, and a minimum bandpass for storing one or a plurality of the bandpass filter group candidates. Selecting an optimum bandpass filter group from the filter storage means and one or a plurality of bandpass filter group candidates, and selecting the first bandpass filter group, the second bandpass filter group, or the third bandpass filter group Or a reliable bandpass filter selection means for outputting the fourth bandpass filter group. It is characterized in that to obtain.

  Further, in the spectrum decoding apparatus, the minimum bandpass filter selection means includes a bandpass filter combination selection means for sequentially outputting one set of all the bandpass filter groups that can be selected from the all bandpass filters, and the bandpass filter. It is determined that the input substance can be discriminated by the decode possibility determination means for determining whether the input substance can be discriminated using a band pass filter group output by the combination selection means, and the decode possibility determination means. A decodable bandpass filter storage unit for storing a bandpass filter group, and a minimum number selection unit for selecting the bandpass filter group candidate from the bandpass filter groups stored in the decodable bandpass filter storage unit It is characterized by comprising .

  Further, in the spectrum decoding apparatus, the minimum band pass filter selecting means includes a number determining means for determining the number of band pass filters of the band pass filter group selected by the band pass filter combination selecting means, and the number determining means selecting the number of band pass filters. An initial number determination means for determining an initial value of the number of bandpass filters, wherein the number determination means is a bandpass filter provided by the bandpass filter combination selection means from the number determination means among all the bandpass filters. All the bandpass filter groups that can be selected by the number of sheets are sequentially output to the decoding possibility determination unit, and the decoding possibility determination unit determines whether all the bandpass filter groups output by the bandpass filter combination selection unit can be determined. Decoding is possible after judgment If at least one band pass filter group exists in the decodable band pass filter storage means, the number of band pass filters that is one less than the number of band pass filters is output. The number of band-pass filters that is one more than the number of band-pass filters is output.

  Further, in the spectrum decoding device, the minimum bandpass filter selection means includes the number of codes of the past input code group, the number of bandpass filters of the bandpass filter group selected by the reliable bandpass filter selection means, and the number of codes. The apparatus further comprises past bandpass filter number storage means for storing the frequency of the number of bandpass filters, wherein the initial number determination means is a code in which the number of codes of the input code group is stored in the past bandpass filter number storage means. When the number is the same as the number, the most frequent number of band-pass filters corresponding to the number of codes is output to the initial number determination means.

  Further, in the spectrum decoding device, the past band-pass filter number storage means limits a number for storing the number of band-pass filters for the number of codes of the past input code group.

  As described above, according to the spectrum decoding apparatus of the present invention, a minimum bandpass filter necessary for decoding one or more coded substances is derived, and measurement is performed using the bandpass filter. Since the number of shootings can be reduced, the measurement time can be increased without reducing the decoding accuracy.

  Embodiments of a spectrum decoding apparatus according to the present invention will be described below in detail with reference to the drawings.

  Embodiments of the present invention described in claims 1, 6, and 7 of the present invention will be described below with reference to FIGS. 1 to 3, 11, and Table 1. FIG.

  FIG. 1 shows a block diagram of a spectrum decoding apparatus in Embodiment 1 of the present invention. In FIG. 1, the spectrum decoding apparatus of the present invention comprises a bandpass filter selection means 101, a reference spectrum data storage means 1101, a decoding means 1116, and a spectrum acquisition means 1117. A controller 1102, an image processing unit 1115, a well plate 1103, a well plate driving unit 1104, a focusing unit 1106, a band pass filter 1109a to a band pass filter 1109h, a rotation filter 1110, a rotation filter driving unit 1111, The objective lens 1105, dichroic mirror 1114, excitation light source 1107, excitation light source control means 1108, CCD camera 1112, and CCD camera control means 1113 are configured.

  Reference spectrum data storage means 1101, system controller 1102, well plate 1103, well plate drive means 1104, focus means 1106, rotary filter drive means 1111, objective lens 1105, dichroic mirror 1114, excitation The light source 1107, the excitation light source control means 1108, the CCD camera 1112, the CCD camera control means 1113, the image processing means 1115, and the decoding means 1116 are the same as those in the conventional configuration, and therefore, the same reference numerals are given for explanation. Omitted.

  The shape of the rotary filter 1110 is a wheel type, and bandpass filters 1109a to 1109h are provided on the circumference. Eight types of bandpass filters are prepared, and each bandpass filter 1109a to bandpass filter 1109h is a bandpass filter that transmits light of a specific wavelength band. The wavelength band of light that is sequentially transmitted from the bandpass filter 1109a becomes shorter, and the bandpass filter 1109h is a bandpass filter that transmits the shortest wavelength band among the filters. These eight types of band-pass filters are prepared in the number necessary for decoding the bead group.

  The band-pass filter selection unit 101 is a minimum band-pass filter group necessary for decoding these from the code and reference spectrum data stored in the reference spectrum data storage unit 1101 (hereinafter referred to as a first band-pass filter group). Select. Here, a combination of a plurality of bandpass filters is referred to as a bandpass filter group, and the bandpass filter group includes, for example, a bandpass filter 1109d, a bandpass filter 1109e, and a bandpass filter 1109f.

  FIG. 2 is a block diagram showing in detail the bandpass filter selection means 101 in FIG. In FIG. 2, the minimum band-pass filter selection unit 200 selects one or a plurality of band-pass filter groups that can decode the code stored in the reference spectrum data storage unit 1101 and includes a minimum number of band-pass filters. To do.

  Further, the minimum band pass filter selection unit 200 includes a band pass filter combination selection unit 201, a decoding possibility determination unit 202, a decodable band pass filter storage unit 203, and a minimum number selection unit 204.

  Now, it is assumed that the reference spectrum data storage unit 1101 stores reference spectrum data of codes A to D as shown in FIG. The bandpass filter combination selection unit 201 selects all combinations of the bandpass filter groups from the eight types of all bandpass filters, and sends the selected bandpass filter group information to the decoding possibility determination unit 202 as needed. For example, a bandpass filter group including a bandpass filter 1109d, a bandpass filter 1109e, and a bandpass filter 1109f is selected.

  The decoding possibility determination unit 202 determines whether the code stored in the reference spectrum data storage unit 1101 can be decoded using the bandpass filter group selected by the bandpass filter combination selection unit 201.

  Details will be described with reference to FIG. FIG. 3 is a diagram showing the reference spectrum data of code A and code B, and the luminance range 1201 defines a predetermined width that can be arbitrarily set around the luminance value of the reference spectrum data.

  First, reference spectrum data of two codes stored in the reference spectrum data storage unit 1101, for example, code A and code B are extracted.

  Next, in the reference spectrum data of code A and code B, at least one or more of the bandpass filter group selected by the bandpass filter combination selection unit 201, the bandpass filter 1109d, the bandpass filter 1109e, and the bandpass filter 1109f. If the luminance ranges 1201 of the luminance values in the bandpass filters of the first and second bandpass filters do not overlap, it is determined that code A and code B can be decoded by this bandpass filter group. In the case of FIG. 3, the code A and code B luminance ranges 1201 overlap in the band pass filter 1109e, but the code A and code B luminance ranges 1201 do not overlap in the band pass filter 1109d and the band pass filter 1109f. Therefore, it is determined that code A and code B can be decoded by this band pass filter group.

  Further, the decoding possibility determination unit 202 performs the above-described determination on all codes stored in the reference spectrum data storage unit 1101 as well as the codes A and B, and if all the codes can be decoded with each other, the band pass filter combination The bandpass filter group selected by the selection unit 201 is determined to be a decodable bandpass filter group.

  Here, the luminance range 1201 may be a constant value for all bandpass filters and all codes, may be a variable value for each bandpass filter, or may be a variable value for each code. In addition, reference spectrum data is acquired multiple times or by measuring multiple beads with the same code, and the standard deviation is calculated from the value obtained by subtracting the standard deviation from the average luminance value for each bandpass filter. The luminance range 1201 may be set up to the added value.

  Table 1 is a table showing whether or not the bandpass filter 1109d, the bandpass filter 1109e, and the bandpass filter 1109f in FIG. 12 can be decoded.

  As shown in Table 1, since the bandpass filter group including the bandpass filter 1109d, the bandpass filter 1109e, and the bandpass filter 1109f is determined to be mutually decodable in the codes A to D, it is stored in the reference spectrum data storage unit 1101. It is determined that the code can be decoded. Then, the decodeability determination unit 202 stores the bandpass filter group determined to be decodable in the decodable bandpass filter storage unit 203.

  The decoding possibility determination unit 202 determines whether the bandpass filter group selected by the bandpass filter combination selection unit 201 can be decoded in the same manner. In FIG. 12, a band-pass filter group having one band-pass filter cannot be decoded, but there are two or more band-pass filter groups that can be decoded. Then, all the decodable bandpass filter groups are stored in the decodable bandpass filter storage unit 203.

  The minimum number selection unit 204 selects one or a plurality of the minimum number of bandpass filter groups from one or a plurality of bandpass filter groups stored in the decodable bandpass filter storage unit 203, and stores the minimum bandpass filter. Store in the means 205. In the case of FIG. 12, the minimum number is two in the band pass filter group stored in the decodable band pass filter storage means 203. The two bandpass filter groups stored in the decodable bandpass filter storage unit 203 are a bandpass filter group including a bandpass filter 1109a and a bandpass filter 1109b, a bandpass filter 1109a, and a bandpass filter 1109c. A bandpass filter group, a bandpass filter group consisting of a bandpass filter 1109a and a bandpass filter 1109d, and a bandpass filter group consisting of a bandpass filter 1109a and a bandpass filter 1109e. The minimum number selection unit 204 stores these band pass filter groups in the minimum band pass filter storage unit 205.

  The reliable bandpass filter selection unit 206 first selects one bandpass filter group from the plurality of bandpass filter groups stored in the minimum bandpass filter storage unit 205. Next, luminance data in the band-pass filter that constitutes the band-pass filter group is extracted from the reference spectrum data of each code stored in the reference spectrum data storage unit 1101.

  The luminance data is compared in each bandpass filter, and when the luminance range 1201 of each code does not overlap, a luminance range interval 1202 that is the interval between the luminance range 1201 of each code and the luminance range 1201 is calculated, and the luminance range interval 1202 Is the minimum luminance range interval of the bandpass filter group. Then, the band pass filter group having the largest minimum luminance range interval is selected. The numbers written beside the luminance range interval 1202 in FIG. 12 are values of the respective luminance range intervals in FIG. According to FIG. 12, the bandpass filter group having the largest minimum luminance range interval is a bandpass filter group including a bandpass filter 1109a and a bandpass filter 1109d.

  In this way, the bandpass filter selection unit 101 selects one of the minimum bandpass filter groups that can discriminate a plurality of codes stored in the reference spectrum data storage unit 1101, and the system controller is selected as the first bandpass filter group. 1102 is output.

  The decoding unit 1116 first extracts reference spectrum data of one code from the measured spectrum data created by the image processing unit 1115 and the reference spectrum data stored in the reference spectrum data storage unit 1101. Next, luminance data in one bandpass filter having the first bandpass filter group selected by the bandpass filter selection unit 101 is extracted from the extracted reference spectrum data and measurement spectrum data, and the luminance data of the measured spectrum data is the reference spectrum. It is determined whether the brightness data is within the brightness range 1201 of the data. This determination is also performed for the other bandpass filters in the first bandpass filter group. If all the determination results are within the luminance range 1201, it is determined that the reference spectrum data and the measured spectrum data match. If the extracted reference spectrum data and the measured spectrum data do not match, the reference spectrum data of other codes are sequentially extracted until they match, and the same determination is made.

  The operation of the spectrum decoding apparatus configured as described above will be described in detail.

  The reference spectrum data storage unit 1101 stores a bead code arbitrarily selected by the user from the bead group and its reference spectrum data. The code of the beads injected into the observation target (hereinafter referred to as a code group) is selected by the user from the codes stored in the reference spectrum data storage unit 1101.

  At this time, the bandpass filter selection unit 101 selects the minimum number of first bandpass filters by the above-described method. Here, it is assumed that a bandpass filter group including the bandpass filter 1109a and the bandpass filter 1109d is selected as the first bandpass filter group.

  Next, the system controller 1102 moves the well plate 1103 in the X-axis or Y-axis direction so that the observation target fixed to the well plate 1103 falls within the imaging range of the CCD camera 1112 using the well plate driving unit 1104. The focus lens 1106 is used to move the objective lens 1105 so that the beads in the observation target are in focus. Furthermore, the system controller 1102 moves the first bandpass filter of the first bandpass filter group selected by the bandpass filter selection unit 101, for example, the bandpass filter 1109a in this case, to the imaging range using the rotary filter driving unit 1111. .

  Next, the system controller 1102 controls the excitation light of the excitation light source 1107 using the excitation light source control means 1108 and outputs the excitation light. The excitation light is reflected in the direction of the observation target by the dichroic mirror 1114 and condensed on the observation target by the objective lens 1105. By irradiating the excitation light, the beads in the observation target emit light, and the emitted light is transmitted through the dichroic mirror 1114, and only the light in a specific wavelength band is transmitted by the band pass filter 1109 a and is incident on the CCD camera 1112. The system controller 1102 controls the exposure of the CCD camera 1112 using the CCD camera control unit 1113, captures the emitted light from the incident beads within the observation target, and sends the captured image to the image processing unit 1115.

  When the first image is captured, the system controller 1102 switches to the second bandpass filter 1109d of the first bandpass filter group selected by the bandpass filter selection unit 101 using the rotation filter driving unit 1111 and switches to one sheet. Take a photo similar to an eye image.

  In this way, when the photographing of all the bandpass filters of the first bandpass filter group is completed, the image processing unit 1115 removes all the beads appearing in the image from the plurality of images sent from the CCD camera control unit 1113. Detection is performed by a technique such as pattern matching, luminance data is calculated for each bead in the image, and a measurement spectrum is calculated.

  Next, the decoding unit 1116 extracts reference data of one code, for example, reference data of code A, from the measured spectrum data of the beads of the image and the reference spectrum data stored in the reference spectrum data storage unit 1101. Next, first, the brightness value in the bandpass filter 1109a is extracted from the measured spectrum data and the reference spectrum data, and it is determined whether the brightness value of the measured spectrum data is within the brightness range 1201 of the brightness value of the reference spectrum. This determination is also performed for the other bandpass filters in the first bandpass filter group. If all of the determination results are within the luminance range 1201, it is determined that the reference spectrum data and the measured spectrum data match, and the code of this bead is specified. If the extracted reference spectrum data and the measured spectrum data do not match, the other reference spectrum data and the reference spectrum data of codes BD are sequentially extracted until they match, and similarly determined.

  The operation in which the bandpass filter selection unit 101 as described above selects the minimum necessary first bandpass filter group that can decode the code stored in the reference spectrum data storage unit 1101 in the reference spectrum data storage unit 1101. It is only necessary to perform an increase / decrease in the stored code. Until the increase / decrease occurs, the observation target is decoded using the most recently selected bandpass filter group.

  As described above, in the first embodiment, the band-pass filter selecting unit 101 selects the minimum necessary first band-pass filter group capable of decoding the code stored in the reference spectrum data storage unit 1101 and selects this. Since the number of photographing can be reduced by decoding the beads in the observation target using the first bandpass filter group thus made, the measurement time can be shortened.

  Embodiments of the present invention described in claims 2, 8, 9, and 10 of the present invention will be described below with reference to FIGS. 4 to 7.

  FIG. 4 is a block diagram of the spectrum decoding apparatus according to the second embodiment of the present invention.

  The difference from the configuration of the first embodiment is that an object information storage unit 401 is provided, and the bandpass filter selection unit 402 selects a bandpass filter group from information in the object information storage unit 401 and the reference spectrum data storage unit 1101. It is.

  FIG. 5 shows the well plate 1103 in detail. In FIG. 5, wells 501 are holes arranged in a grid on the well plate 1103, and here, the observation target is the well 501. Each well 501 is assigned a number. For example, the well plate 1103 has 384 wells 501 in the X direction and 16 in the Y direction, and the numbers are assigned from 1 to 24 in the X direction. If the numbers are assigned from A to P in the Y direction, the well numbers are indicated by A1 to P24 using the numbers in the X direction and the Y direction.

  In FIG. 4, the subject information storage unit 401 stores at least one code group in the well to be measured, which is input by the user.

  The bandpass filter selection unit 402 is a bandpass capable of decoding the beads of the code group stored by the subject information storage unit 401 from the code group stored by the subject information storage unit 401 and the reference spectrum data corresponding to the code group. A filter group (hereinafter referred to as a second bandpass filter group) is selected.

Next, the operation will be described.
Here, as the measurement of the well B1, the well D8, and the well E12 from now on, the subject information storage unit 401 stores the code group of the well B1, the well D8, and the well E12 input by the user.

  The bandpass filter selection unit 402 extracts the reference spectrum data of the code group of the well B1 used in the next measurement of the subject information storage unit 401 from the reference spectrum storage unit 1101, and thereafter the well B1 as in the first embodiment. The band pass filter group capable of decoding the beads of the code group is selected and output to the system controller 1102 as the second band pass filter group.

  Thereafter, similarly to the first embodiment, the spectrum acquisition unit 1117 acquires the measurement spectrum data of the well B1, and the decoding unit 1116 performs decoding.

  Similarly to the measurement of the well B1, the band-pass filter selection unit 402 selects the smallest second band-pass filter group capable of decoding the code group of each well D8 and E12, and performs measurement.

  As described above, in this embodiment, the subject information storage unit 401 stores the code group in the well to be measured, so that the bandpass filter can be selected according to the code group in each well 501, and the measurement is performed. Since the number of bandpass filters to be used can be reduced, the measurement time can be shortened.

  In the second embodiment, the bandpass filter selection unit 402 may be configured as a block diagram as shown in FIG.

  In FIG. 6, the minimum band pass filter selection means 600 includes an initial number determination means 603, a number determination means 602, a band pass filter combination selection means 601, a decoding possibility determination means 202, a decodable band pass filter storage means 203, and a minimum number selection means. The initial number determination means 603 determines the first number of band-pass filters selected by the number determination means 602.

  The sheet number determining means 602 is a means for determining the number of bandpass filter groups selected by the bandpass filter combination selecting means 601. The first determined number is the number of bandpass filters determined by the initial number determining means 603.

  The bandpass filter combination selection unit 601 selects the bandpass filters for the number of bandpass filters determined by the number determination unit 602 from the eight bandpass filters.

  The decoding possibility determination unit 202, the decodable bandpass filter storage unit 203, the minimum number selection unit 204, the minimum bandpass filter storage unit 205, and the reliable bandpass filter selection unit 206 are the same as those in the first embodiment. The same reference numerals are given and description thereof is omitted.

Next, the operation will be described.
The initial number determining unit 603 determines the first number of band-pass filters selected by the number determining unit 602, for example, four. In the first determination, the number determination unit 602 determines four of the bandpass filter numbers determined by the initial number determination unit 603. The bandpass filter combination selection unit 601 selects an arbitrary four bandpass filter groups from the eight bandpass filters. The decoding possibility determination unit 202 determines whether the code group can be decoded by the bandpass filter group selected by the bandpass filter combination selection unit 601, and stores it in the decodable bandpass filter storage unit 203 if decoding is possible. . Thereafter, the bandpass filter combination selection unit 601 sequentially selects all bandpass filter groups including four bandpass filters and performs the same processing. When the bandpass filter combination selection unit 601 has sequentially selected all the bandpass filter groups including the four bandpass filters, the number determination unit 602 enters the second determination.

  First, the number determination means 602 checks whether a band pass filter group is stored in the decodable band pass filter storage means 203. If the band pass filter group is stored, the number obtained by subtracting one from the previously determined number is determined, and if not stored, the number obtained by adding one to the previously determined number is determined.

  Subsequent operations differ depending on whether the number of sheets determined by the number determination unit 602 for the second time is the number obtained by subtracting one from the previously determined number or the number obtained by adding one to the previously determined number.

  First, when the number of sheets determined by the number-of-sheets determining unit 602 is the number obtained by subtracting one from the number of times determined last time, the band-pass filter combination selecting unit 601 and the number of sheets determined for the second time are the same as in the first time. A decodable band pass filter group is determined using the decodability determination unit 202 and is additionally stored in the decodable band pass filter storage unit 203 as the first storage information. The number determination means 602 enters the third number determination, but if there is a previously determined number of bandpass filter groups in the decodable bandpass filter storage means 203, determines the number obtained by subtracting one from the previously determined number. If it is the same as the second time, the number determination is terminated. When the decodable bandpass filter storage unit 203 does not have the previously determined number of bandpass filter groups and the number determination unit 602 ends, the minimum number selection unit 204 stores the minimum number of bandpass filters from the decodable bandpass filter storage unit 203. Select a group.

  Next, when the number of sheets determined by the sheet number determination unit 602 is the number obtained by adding one to the number determined last time, the sheet number determination unit 602 stores the bandpass filter group in the decodable bandpass filter storage unit 203. Until it is confirmed that the number is determined, the number obtained by adding one is determined from the previously determined number. If it is confirmed that there is a bandpass filter group, the number determination unit 602 is terminated.

  When the number-of-sheets determining unit 602 ends, the minimum number of sheets selection unit 204 thereafter selects the minimum number of bandpass filter groups from the decodable bandpass filter storage unit 203 as in the first embodiment, and the minimum number of bandpass filter storage units 205. To remember. The reliable bandpass filter selection unit 206 selects a bandpass filter group having the largest minimum luminance range interval from the bandpass filter group stored in the minimum bandpass filter storage unit 205.

  As described above, the number determination unit 602 determines the number of bandpass filters of the bandpass filter group selected by the bandpass filter combination selection unit 601 using the determination result of the decoding possibility determination unit 202 in the previous number of bandpass filters. By determining the bandpass filter group to be selected by the bandpass filter selection unit 402, it is possible to reduce the number of bandpass filter groups for determining whether or not decoding is possible, so the processing of the bandpass filter selection unit 502 can be performed at high speed. It can be carried out.

  Further, the bandpass filter selection unit 402 of the second embodiment may be a block diagram as shown in FIG.

  The block diagram shown in FIG. 7 is different from the block diagram shown in FIG. 6 in that a past band-pass filter number storage unit 702 is provided. In FIG. 7, the minimum band pass filter selection means 700 includes a past band pass filter number storage means 702, an initial number determination means 701, a number determination means 602, a band pass filter combination selection means 601, a decode availability determination means 202, and a decodable band pass. The filter storage unit 203 and the minimum number selection unit 204 are configured. The past bandpass filter number storage unit 702 includes the number of codes in the code group of the next well stored in the subject information storage unit 401 and The number of band pass filters of the band pass filter group selected in the past in the code group is stored.

Next, the operation in the block diagram of FIG. 7 will be described.
First, the initial number determination means 701 is selected from the number of bandpass filters corresponding to the number of codes in the well code group to be measured next stored in the subject information storage means 401 from the bandpass filter number storage means 702. The number of bandpass filters is determined as the initial number of bandpass filters.

  Thereafter, the operation until the reliable bandpass filter selection unit 703 selects the bandpass filter group is the same as the operation in FIG.

  The reliable bandpass filter selection means 703 sends the selected bandpass filter group to the system controller 1102 and simultaneously sends it to the past bandpass filter number storage means 702, and the past bandpass filter number storage means 702 sends the received bandpass filter. The number of bandpass filters in the filter group and the number of codes in the next well code group are stored.

  As described above, by determining the number of band-pass filters most frequently selected in the past with respect to the number of codes by the initial number determination means 701, the determination is performed from the number of band-pass filters most likely to be selected according to the number of codes. The processing of the bandpass filter selection unit 402 can be performed at higher speed in accordance with the use status of the user's code.

  In FIG. 7, the number of past bandpass filter number storage means 702 that stores the number of past bandpass filter numbers relative to the number of codes can also be limited.

  For example, there are two code groups of 8 codes, code group M and code group N, and the number of bandpass filters of the bandpass filter group selected by the bandpass filter selection unit 402 for these code groups is 6 respectively. Suppose that there are 3 sheets. Assume that the measurement of 30 wells of the cord group M has been completed.

  At this time, if the past band-pass filter number storage unit 702 does not limit the number of past band-pass filter numbers stored with respect to the number of codes, the past band-pass filter number storage unit 702 determines that the number of codes is 8 and the number of band-pass filters. It is stored that the number of selections of 6 sheets is 30. Next, when one well of the code group N is measured, the number of codes in the past band-pass filter number storage means 702 is 8, and the number of selections of 6 band-pass filters is 30. It is stored that the number of selected sheets is one. At this time, since the number of band-pass filters with the largest number of selections in code number 8 is 6, the initial number determination means 701 sets the initial number of band-pass filters to 6. Further, when the wells of the code group N are measured, the number of selections of 3 band-pass filters increases to 2 times and 3 times. Further, the wells to be observed in the code group N are measured, and when the number of selections of 3 bandpass filters is 31, the selection number of 6 bandpass filters is 30 and the number of bandpass filters is 3 The number of times of selection is 31. That is, the number of band-pass filters with the largest number of selections in code number 8 is three. Therefore, the initial number determination unit 701 sets the initial number of bandpass filters to three.

  On the other hand, the case where the number of past bandpass filter number storage means 702 stores the number of past bandpass filter numbers with respect to the number of codes will be described below. For example, the number of past bandpass filter number storage means 702 that stores the number of past bandpass filter numbers relative to the number of codes is 10, and the number of bandpass filter numbers corresponding to each number of codes is stored in the past bandpass filter number storage means 702. A first-in first-out memory (hereinafter referred to as a FIFO memory) is prepared. FIG. 8 shows a FIFO memory for the number of codes of 8 in the past bandpass filter number storage means 702.

  Now, assuming that 30 wells of the code group M have been measured, all six band-pass filters are included in the FIFO memory as shown in FIG. Next, when one well of the code group N is measured, the number of bandpass filters stored in FIG. 8A is shifted to the output side in the FIFO memory in the code number 8 as shown in FIG. 8B. Three new band-pass filters are input. Note that the information on the number of bandpass filters output by shifting is deleted. At this time, since the most number of band pass filters stored in the FIFO memory in the code number 8 is 6, the initial number determination means 701 sets the number of initial band pass filters to 6. Further, when the wells of the code group N are measured, the number of band-pass filters is newly input to the FIFO memory and the number of band-pass filters is output and deleted. Further, when the wells of the code group N are measured and the sixth measurement of the wells of the code group N is performed, the FIFO memory in the code number 8 is changed from the state of FIG. 8 (a) as shown in FIG. 8 (c). Three band-pass filter sheets are input, and six band-pass filter sheet numbers are output. That is, the most number of band-pass filters stored in the FIFO memory with the code number 8 is three. Therefore, the initial number determination unit 701 sets the initial number of bandpass filters to three.

  As described above, when the past band-pass filter number storage unit 702 does not limit the number of past band-pass filter numbers stored with respect to the number of codes, the initial number determination unit 701 starts the 31st measurement of the wells of the code group N for the first time. In the case where the number of initial bandpass filters selected from the above is changed from six to three, the number of past bandpass filters stored in the past bandpass filter number storage unit 702 is limited. The number of initial bandpass filters selected by the initial number determination means 701 is changed from six to three at the sixth time after the measurement of the wells of the code group N is started for the first time.

  As described above, by limiting the number of past bandpass filter number storage means 702 that stores the number of past bandpass filters with respect to the number of codes, the user's usage situation is tracked, and the processing of the bandpass filter selection means 402 is performed. It can be done faster.

  An embodiment of the invention described in claim 3 of the present invention will be described below with reference to FIG.

  FIG. 9 is a block diagram of the spectrum decoding apparatus according to the third embodiment of the present invention.

  The difference from the configuration of the second embodiment is that a discriminated subject information storage unit 902 and a selector 903 are provided.

  In FIG. 9, the discriminated subject information storage unit 902 stores the discriminated well code group and the second bandpass filter group selected by the bandpass filter selecting unit 402 based on the code group.

  The selector 903 compares the determined well code group stored in the determined subject information storage means 902 with the well code group to be determined next stored in the subject information storage means 901, and both code groups are the same. If there is, a band pass filter group corresponding to the code group stored in the discriminated subject information storage unit 902 is selected and output to the system controller 1102 as a third band pass filter group. When the code group of the well to be discriminated next stored in the subject information storage unit 901 is different from the code group of the discriminated well, or the discriminated well code group is stored in the discriminated subject information storage unit 902. If not, the bandpass filter selection unit 402 is instructed to select a second bandpass filter group that can decode the code group output from the subject information storage unit 901, and the second band selected by the bandpass filter selection unit 402 is selected. The pass filter group is output to the system controller 1102 as a third band pass filter group, and stored in the subject information storage unit 901 in the discriminated subject information storage unit 902 and the code group of the well to be discriminated next is selected. A band-pass filter group is stored.

Next, the operation of the third embodiment will be described.
First, the subject information storage unit 901 stores at least one code group in the well to be measured, which is input by the user.

  The selector 903 then selects a band-pass filter selection unit based on the determined well code group stored in the determined subject information storage unit 902 and the well code group to be determined next stored in the subject information storage unit 901. Either the second band-pass filter group output by 402 or the band-pass filter group output by the discriminated subject information storage unit 902 is selected. In the subsequent operation, the spectrum acquisition unit 1117 acquires the measured spectrum data, and the decoding unit 1116 performs decoding, as in the first embodiment.

  As described above, in the third embodiment, the determined well code group stored in the determined subject information storage unit 902 is compared with the code group of the well to be determined next stored in the subject information storage unit 901. By determining the bandpass filter group to be selected based on the result, if the well to be discriminated next stored in the subject information storage unit 901 and the well of the discriminated subject information storage unit 902 are the same code group, the bandpass filter Since the processing of the selection unit 402 can be omitted, measurement can be performed at high speed.

  Hereinafter, an embodiment of the invention described in claim 4 of the present invention will be described with reference to FIG.

  FIG. 10 is a block diagram of a spectrum decoding apparatus according to another embodiment 4 of the present invention.

  The difference from the configuration of the first embodiment is that the decoding result of the decoding unit 1002 is output to the bandpass filter selection unit 1001.

The operation will be described with reference to FIG.
When two or more wells 501 on the well plate 1103 contain the same code group in succession, for example, when the same code group is contained in well A1 to well A5, and these five wells are continuously measured. First, the bandpass filter selection unit 1001 selects a bandpass filter group using eight bandpass filters when measuring the well A1 of the first well, and outputs it to the spectrum acquisition unit 1117 as the fourth bandpass filter group. To do. Thereafter, as in the first embodiment, the spectrum acquisition unit 1117 acquires the measurement spectrum of the well A1. The decoding unit 1002 performs decoding based on the decode measurement spectrum, and outputs the decoding result to the bandpass filter selection unit 1001. From the decoding result of the decoding means 1002, not only the well A1, but also the code groups in the well A2 to the well A5 are known.

Next, the measurement proceeds to the second well, well A2. The bandpass filter selection unit 1001 selects the fourth bandpass filter group based on the code group output from the decoding unit 1002. Thereafter, measurement is performed in the same manner as in the first well.
The measurement after the third well is performed in the same manner as the second well.

  As described above, in the fourth embodiment, when a well having the same code group is continuously measured more than once, the decoding result measured in the first well is used in the measurement after the second well, so that the user can select the well code. Since the number of band pass filters used for measurement can be reduced without performing group input, measurement can be performed at high speed.

  Further, when the code group of the first well includes all the code groups of the second and subsequent wells, the fourth band selected by the bandpass filter selection unit 1001 based on the code group of the decoding result measured in the first well The second and subsequent wells can be discriminated using the passfill group.

  For this reason, even when the code group of the first well includes all the code groups of the second well and later, the decoding result measured in the first well is used in the measurement of the second well and later, so that the user can use the code group of the well. Since the number of band-pass filters used for measurement can be reduced without performing input, measurement can be performed at high speed.

  The spectrum decoding apparatus according to the present invention is suitable for increasing the measurement speed when discriminating types of substances having different luminance spectrum distributions.

  Moreover, the spectrum decoding apparatus concerning this invention is applicable also to the use of a gene expression analyzer by using it as a fluorescence microscope etc.

1 is a block diagram of a spectrum decoding apparatus according to a first embodiment of the present invention. FIG. 1 is a detailed block diagram of a bandpass filter selection unit of the spectrum decoding apparatus according to the first embodiment of the present invention. Reference spectrum data diagram of code A and code B in Embodiment 1 of the present invention Block diagram of spectrum decoding apparatus in embodiment 2 of the present invention The enlarged view of the well plate in Example 2, Example 3, and Example 4 of this invention Detailed block diagram of bandpass filter selection means of spectrum decoding apparatus in embodiment 2 of the present invention Detailed block diagram of bandpass filter selection means of spectrum decoding apparatus in embodiment 2 of the present invention The figure of FIFO memory in the code number 8 of the past band pass filter number storage means in Example 2 of this invention Block diagram of spectrum decoding apparatus in embodiment 3 of the present invention Block diagram of spectrum decoding apparatus in embodiment 4 of the present invention Block diagram of a conventional spectrum decoding device The figure which shows an example of the reference spectrum data in the conventional spectrum decoding apparatus, Example 1, Example 2, Example 3, Example 4 of this invention The figure which shows an example of the measurement spectrum data in the conventional spectrum decoding apparatus

Explanation of symbols

DESCRIPTION OF SYMBOLS 101 Band pass filter selection means 200 Minimum band pass filter selection means 201 Band pass filter combination selection means 202 Decoding possibility judgment means 203 Decodable band pass filter storage means 204 Minimum number selection means 205 Minimum band pass filter storage means 206 Reliable band pass filter Selection means 401 Object information storage means 402 Band pass filter selection means 501 Well 600 Minimum band pass filter selection means 601 Band pass filter combination selection means 602 Number determination means 603 Initial number determination means 700 Minimum band pass filter selection means 701 Initial number determination Means 702 Past band pass filter number storage means 703 Trust band pass filter selection means 901 Subject information storage means 902 Discriminated subject information Storage unit 903 Selector 1001 Band pass filter selection unit 1002 Decoding unit 1101 Reference spectrum data storage unit 1102 System controller 1103 Well plate 1104 Well plate driving unit 1105 Objective lens 1106 Focus unit 1107 Excitation light source 1108 Excitation light source control unit 1109a Band pass filter 1109b Band Pass filter 1109c Band pass filter 1109d Band pass filter 1109e Band pass filter 1109f Band pass filter 1109g Band pass filter 1109h Band pass filter 1110 Rotation filter 1111 Rotation filter drive means 1112 CCD camera 1113 CCD camera control means 1114 Dichroic mirror 11 5 the image processing unit 1116 decoding means 1117 spectrum acquiring unit 1201 luminance range 1202 brightness range interval

Claims (10)

A spectrum decoding device for measuring the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and discriminating the type of the substance, Luminance spectrum distribution (hereinafter referred to as reference spectrum data) inherent to a plurality of substances arbitrarily selected from the substances (hereinafter referred to as substance groups) prepared in advance so as to be discriminated by differences and the reference Reference spectrum data storage means for storing a code corresponding to the spectrum data in advance, reference spectrum data is taken out from the reference spectrum data storage means, and the plurality of pieces prepared for determining the substance group are prepared. Bandpass filter Bandpass filter selecting means for selecting a minimum bandpass filter (hereinafter referred to as a first bandpass filter group) necessary for discriminating the plurality of substances from the plurality of substances, and the plurality of substances A spectrum acquisition means for acquiring a luminance spectrum distribution of a plurality of substances (hereinafter referred to as a plurality of analytes) from which all or a part of the spectrum is extracted using the first bandpass filter group, and the spectrum acquisition means A spectrum decoding apparatus comprising: a decoding unit that compares the acquired luminance spectrum distribution with the reference spectrum data and discriminates the plurality of subjects. A spectrum decoding device for measuring the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and discriminating the type of the substance, Luminance spectrum distribution (hereinafter referred to as reference spectrum data) inherent to a plurality of substances arbitrarily selected from the substances (hereinafter referred to as substance groups) prepared in advance so as to be discriminated by differences and the reference Reference spectrum data storage means for storing a code corresponding to spectrum data in advance, and a plurality of substances corresponding to a plurality of substances extracted from all or a part of the plurality of substances (hereinafter referred to as a plurality of subjects). Object information storage means for storing in advance a code (hereinafter referred to as a code group), Based on the code group stored in the object information storage means, the reference spectrum data corresponding to the plurality of objects is extracted from the reference spectrum data storage means, and the number of sheets necessary for discriminating the substance group is prepared. A bandpass filter (hereinafter referred to as a second bandpass filter group) that is minimally necessary for discriminating the plurality of subjects from the plurality of bandpass filters (hereinafter referred to as all bandpass filters). A bandpass filter selection unit to select; a spectrum acquisition unit to acquire a luminance spectrum distribution of the plurality of subjects using the second bandpass filter group; and a luminance spectrum distribution acquired by the spectrum acquisition unit to the reference spectrum Decoding means for discriminating the plurality of subjects in comparison with data; Spectrum decoding apparatus characterized by comprising a. A spectrum decoding device for measuring the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and discriminating the type of the substance, Luminance spectrum distribution (hereinafter referred to as reference spectrum data) inherent to a plurality of substances arbitrarily selected from the substances (hereinafter referred to as substance groups) prepared in advance so as to be discriminated by differences and the reference Reference spectrum data storage means for storing a code corresponding to spectrum data in advance, and a plurality of substances corresponding to a plurality of substances extracted from all or a part of the plurality of substances (hereinafter referred to as a plurality of subjects). Object information storage means for storing in advance a code (hereinafter referred to as a code group), Based on the code group stored in the object information storage means, the reference spectrum data corresponding to the plurality of objects is extracted from the reference spectrum data storage means, and the number of sheets necessary for discriminating the substance group is prepared. A bandpass filter (hereinafter referred to as a second bandpass filter group) that is minimally necessary for discriminating the plurality of subjects from the plurality of bandpass filters (hereinafter referred to as all bandpass filters). Bandpass filter selection means to select, among the observation objects into which the plurality of subjects have been injected, the code groups of the plurality of subjects to be observed that have been determined, and the code groups for determining the code groups Discriminated subject information storage means for storing a second band pass filter group (hereinafter referred to as a discriminated second band pass filter group); Based on the code group output from the object information storage means, the second bandpass filter group output from the bandpass filter selection means or the determined second band output from the determined object information storage means A selector that selects one of the pass filter groups and outputs the selected band filter group as a third band pass filter group; a spectrum acquisition unit that acquires luminance spectrum distributions of the plurality of subjects using the third band pass filter group; and the spectrum Decoding means for determining the plurality of objects by comparing the luminance spectrum distribution acquired by the acquisition means with the reference spectrum data, and the selector includes the code group output from the object information storage means and the determination If the code groups stored in the completed subject information storage means match, the determined second band pass When the filter group is the third bandpass filter group and the code group output from the subject information storage means and the code group stored in the determined subject information storage means do not match, the bandpass filter selection Means for instructing the means to select the second bandpass filter group, and the second bandpass filter group is used as the third bandpass filter group. A spectrum decoding device for measuring the luminance spectrum distribution using a plurality of bandpass filters having different transmission bands for substances having different luminance spectrum distributions, and discriminating the type of the substance, Luminance spectrum distribution (hereinafter referred to as reference spectrum data) inherent to a plurality of substances arbitrarily selected from the substances (hereinafter referred to as substance groups) prepared in advance so as to be discriminated by differences and the reference Reference spectrum data storage means for storing a code corresponding to spectrum data in advance, and the reference spectrum data storage means from the reference spectrum data storage means, the number of sheets necessary for determining the substance group is prepared Multiple bandpass filters (below) Bandpass filter (hereinafter referred to as a plurality of analytes) extracted from all or part of the plurality of substances (hereinafter referred to as a plurality of analytes) , Referred to as a fourth bandpass filter group), a spectrum acquisition means for acquiring luminance spectrum distributions of the plurality of subjects using the fourth bandpass filter group, and the spectrum acquisition A decoding means for comparing the luminance spectrum distribution acquired by the means with the reference spectrum data to determine the plurality of subjects, and outputting a code group of the plurality of subjects to the bandpass filter selection means, In the case where there are a plurality of observation targets into which the plurality of subjects are injected, When acquiring the luminance spectrum distribution of the plurality of subjects in the first observation target among the observation targets, the all band pass filters are output to the spectrum acquisition means as the fourth band pass filter group, and the second When acquiring the luminance spectrum distribution of the plurality of subjects in the subsequent observation target, the fourth band pass filter group is selected based on the code group output by the decoding means and output to the spectrum acquisition means. A spectrum decoding device characterized by that. The spectrum decoding apparatus according to claim 4, wherein the plurality of subjects in the plurality of observation objects all include the same type of code group. The band-pass filter selection means can discriminate between the plurality of substances or the plurality of analytes (hereinafter referred to as input substances) of an input code (hereinafter referred to as input code group), and a minimum number of bands A minimum band-pass filter selection unit that selects one or a plurality of band-pass filter group candidates configured by a pass filter; a minimum band-pass filter storage unit that stores one or a plurality of band-pass filter group candidates; An optimal bandpass filter group is selected from one or a plurality of bandpass filter group candidates, and the first bandpass filter group, the second bandpass filter group, the third bandpass filter group, or the fourth bandpass is selected. And a reliable band pass filter selecting means for outputting a filter group. 2 and spectrum decoding apparatus according to claim 3 and claim 4. The minimum band pass filter selection means outputs all the band pass filter groups that can be selected from the all band pass filters, one band pass filter combination selection means for sequentially outputting each group, and the band pass filter combination selection means for output. Decodeability determination means for determining whether or not the input material can be determined using a bandpass filter group, and a bandpass filter group determined to be able to determine the input material by the decodeability determination means A decodable bandpass filter storage unit, and a minimum number selection unit for selecting the bandpass filter group candidate from the bandpass filter group stored in the decodable bandpass filter storage unit. The spectrum decoding device according to claim 6. The minimum bandpass filter selection means includes a number determination means for determining the number of bandpass filters of the bandpass filter group selected by the bandpass filter combination selection means, and an initial number of the bandpass filters selected by the number determination means. An initial number determination unit that determines a value, and the number determination unit includes all the bandpass filter combination selection units that can be selected by the number of bandpass filters provided from the number determination unit among all the bandpass filters. The band pass filter groups are sequentially output to the decode enable / disable determining unit, and the decode enable / disable determining unit determines whether or not all the band pass filter groups output by the band pass filter combination selecting unit can be determined. Refer to the bandpass filter storage means, the previous If at least one bandpass filter group exists in the decodable bandpass filter storage means, the number of bandpass filters that is one less than the number of bandpass filters is output, and if not, the band that is one more than the number of bandpass filters is output. 8. The spectrum decoding apparatus according to claim 7, wherein the number of pass filters is output. The minimum bandpass filter selection means includes the number of codes in the past input code group, the number of bandpass filters in the bandpass filter group selected by the reliable bandpass filter selection means, and the number of bandpass filters for each number of codes. A past band pass filter number storage unit for storing the frequency, and the initial number determination unit is configured to store the number of codes in the input code group equal to the number of codes stored in the past band pass filter number storage unit. 9. The spectrum decoding apparatus according to claim 8, wherein the number of band-pass filters most frequently corresponding to the number of codes is output to the initial number determining means. 10. The spectrum decoding apparatus according to claim 9, wherein the past band-pass filter number storage unit limits the number of band-pass filter numbers stored with respect to the past number of codes of the input code group.

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