JP2005513465A5 - - Google Patents

Claims (26)

A body having a first surface and an opposing second surface, wherein at least one channel is formed therein and extends through the body from the first surface to the second surface; A body having a reservoir portion that opens at a surface;
And at least one nozzle arranged along the second surface, the nozzle and the channel in fluid communication, one end of the channel is terminated in a nozzle opening which is formed as part of the nozzle, the device A microfluidic device comprising an injection molded article comprising a nozzle formed from an injection molded article .   The microfluidic device of claim 1, wherein the channel is cylindrically shaped along at least a substantial length thereof, and the channel is defined by a seamless cylindrical surface.   The microfluidic device of claim 1, wherein the channel is internally tapered such that the channel dimension is maximum within the reservoir portion and minimum at the nozzle opening. The microfluidic device of claim 1, wherein the channel is formed in the reservoir portion such that the channel is substantially perpendicular to both the first and second surfaces.   The microfluidic device of claim 1, wherein the at least one nozzle extends beyond the second surface and is substantially conical.   The microfluidic device according to claim 5, wherein an outer diameter of the nozzle is about 50 μm or less.   The microfluidic device according to claim 1, wherein an outer diameter of the nozzle opening is about 50 μm or less.   The microfluidic device of claim 1, wherein a portion of the channel formed in the nozzle and ending in the nozzle opening is tapered inwardly toward the nozzle opening.   The microfluidic device of claim 1, wherein the at least one channel and the at least one nozzle are arranged in a geometric array.   The microfluidic device of claim 1, further comprising a conductive region formed around an outer periphery of at least one nozzle on the second surface. The microfluidic device of claim 10 , wherein the conductive region is formed of a metal and is electrically connected to the electrical contact.   The channel has a first portion whose inner channel surface is parallel, the first portion at least partially defining a reservoir portion and extending to the first surface, the channel further comprising a non-inner channel surface. The microfluidic device of claim 1, comprising a second portion in a parallel relationship, the second portion extending from the first portion to the nozzle opening. A body having a first surface and an opposing second surface, wherein at least one channel is formed therein and extends through the body from the first surface to the second surface, the channel receiving the sample A body having a reservoir portion opening at a first surface for
Is formed in the main body integrally, and at least one nozzle along the second surface is arranged to extend beyond the surface, the nozzle is defined by an outer surface not substantially parallel, the number of the number of nozzles channel Each nozzle has a portion of the resulting channel , each channel ends within the nozzle opening of the nozzle, the nozzle opening diameter is about 100 μm or less, and the nozzle outer diameter is about 150 μm. Ah is, the channel portion formed by said nozzle terminates at one end in with the formation of the nozzle opening is defined by a surface that is not parallel, the microfluidic device comprising a nozzle, a. The microfluidic device of claim 13 , wherein the nozzle opening has a diameter of about 150 μm or less and the nozzle has an outer diameter of about 100 μm or less. 14. The microfluidic device of claim 13 , further comprising a device for sealing the reservoir portion and for transporting the sample from the reservoir portion through the channel to a nozzle opening from which the sample is discharged. A seal in the form of a deformable elastic polymer cover sheet initially disposed over the open end of the reservoir portion, the seal being attached to the first surface and along the first surface Including a seal and a shaft for engaging the polymer cover sheet;
The polymer cover sheet is deformed when the shaft is driven to the extended position, forming a seal with the inner surface of the reservoir portion to force the sample to flow toward the nozzle opening and discharge the sample there. 14. The microfluidic device according to 13 . The transport device comprises a displaceable member, the displaceable member including a base around which a deformable seal extends, the base being initially placed over the open end of the reservoir portion and connected to the base to the shaft. ,
When the shaft is driven to the extended position, the base is received into the reservoir portion and the flange forms a seal with the inner surface of the reservoir portion to force the sample to flow toward the nozzle opening where the sample flows. 16. The microfluidic device according to claim 15, which is released. The transport device comprises a member through which a hole is formed, a gasket disposed at the end of the member, the gasket forming a seal between the member and the reservoir portion;
16. The microfluidic device of claim 15 , wherein the member is introduced into the reservoir portion to communicate with a fluid source that forces the sample to flow toward the nozzle opening and discharges the sample there. A microfluidic device comprising a body and at least one nozzle extending outwardly from the body,
At least one channel is formed within the body, each channel extending through the body from its first surface to its second surface,
Each channel has a reservoir portion that opens at a first surface for receiving a sample, and at least one nozzle extends outwardly from the second surface;
Each nozzle is in fluid communication with one channel, each channel terminating in a nozzle opening formed as part of the nozzle,
The body and at least one nozzle are
Providing a mold including negative impressions of the channel and at least one nozzle;
Injecting a polymer material into the mold;
Curing the polymeric material to form a body in which at least one nozzle extends outwardly from the second surface and at least one channel is formed in the body;
Removing the body from the mold; and a microfluidic device formed by a process. The microfluidic device according to claim 19 , wherein the mold is configured such that the diameter of the nozzle opening of the formed microfluidic device is 100 μm or less and the outer diameter of the nozzle is 150 μm or less. The mold includes a first die and a second die, the first die having a plurality of pins extending outward from the die, the plurality of pins being openings formed in the second die. 20. A microfluidic device according to claim 19 , wherein each opening ends in a closed conical portion. 20. The microfluidic device of claim 19 , further comprising controlling the pressure used to inject the polymer resin to define the nozzle opening so that the polymer material is removed from the region within the gap. 20. The microfluidic fluid of claim 19 , further comprising the step of altering at least the surface properties of the mold portion defining the outer surface of the nozzle to reduce surface friction at the portion and to improve the flow characteristics of the injected resin within the portion. device. A detection system for detecting one or more characteristics of a sample, comprising:
A body having a first surface and an opposing second surface, wherein at least one channel is formed therein and extends through the body from the first surface to the second surface; A body having a reservoir portion that is open at a surface, and at least one nozzle formed integrally with the body and extending along and extending along the second surface, the nozzle being a substantially non-parallel outer surface is defined by the number of nozzles is equal to the number of channels, each nozzle having a portion of the channels that are a result formed, each channel terminates at the nozzle opening formed as part of the nozzle, the outer diameter of the nozzle diameter of the aperture is about 100μm or less nozzles Ri der about 150μm or less, Te channel portion formed by said nozzle ending at one end with the formation of the nozzle opening And injection molding the microfluidic devices formed nozzle, an injection molding material with a defined by the path-shaped inner surface,
A detector for receiving a sample emitted from the microfluidic device through its nozzle opening, the detector analyzing the emitted sample and providing information regarding one or more characteristics of the sample; Including detection system. A device that interfaces with a mass spectrometer to perform nanospray applications,
A microfluidic device comprising a body having a first surface and an opposing second surface, wherein at least one channel is formed within the body and extends through the body from the first surface to the second surface. The channel has a reservoir portion that opens at the first surface and at least one nozzle disposed along the second surface, the nozzle being in fluid communication with the channel, wherein one end of the channel is at the tip of the nozzle A microfluidic device ending in a nozzle opening formed as part of the section;
A frame disposed around the periphery of the microfluidic device to ensure that the microfluidic device is held securely;
A holder having first and second holding members, wherein the first and second holding members are disposed at a sufficient interval, and a frame is disposed between these members, and a predetermined position is set by these members. And a holder in which, in the holding position, at least one nozzle for spraying the sample into the inlet of the mass spectrometer is positioned. A body having a first surface and a second surface opposite the body, the body having at least one channel formed therein, and from the first surface to the second surface, A body extending through the body, the channel having a reservoir portion that is open at the first surface;
At least one nozzle disposed along a second surface and having the resulting channel length so that one end of the channel is formed as a distal end of the nozzle And at least one nozzle, wherein the device is formed from an injection moldable material and the channel length formed by the nozzle has a variable diameter;
A microfluidic device comprising an injection molded article comprising: