JP2005313065A - Cartridge for chemical reaction, manufacturing method therefor, and mechanism for driving cartridge for chemical reaction - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To realize a cartridge for chemical reaction which is excellent in precision and reproduction, and also realize a manufacturing method therefor and a mechanism for driving a cartridge for chemical reaction. <P>SOLUTION: The cartridge for chemical reaction is composed of a rigid-body substrate and a container formed with an elastic body. A plurality of rooms, which are arranged to be connected or possibly connected to each other at a flow path, are formed in the container. External force is applied to the container from outside of the container to move fluid materials in the flow path, the rooms or both to conduct a chemical reaction. In addition, at least a discharging path is provided, which removes the air in the rooms in which the fluid materials flow. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a chemical reaction cartridge, a method for producing the same, and a chemical reaction cartridge driving mechanism, and more particularly to an improvement in a liquid feeding structure related to synthesis, dissolution, detection, separation, and the like of a solution.

Conventionally, test tubes, beakers, pipettes, and the like have been used in processes such as solution synthesis, dissolution, detection, and separation. For example, the substance A and the substance B are collected in a test tube or a beaker, and then poured into a container such as another test tube or a beaker, and the substance C is made by mixing and stirring. For the substance C synthesized in this way, for example, light emission, heat generation, coloration, colorimetry and the like are observed.
Alternatively, the target substance may be separated and extracted by filtering or centrifuging the mixed substance.

Further, the dissolution treatment, for example, the dissolution with an organic solvent is performed using a glass instrument such as a test tube or a beaker. Similarly, in the detection process, the test substance A and the reagent are put in a container and the reaction result is observed.
On the other hand, a bioanalyzer or the like uses a bag called a biochip formed of a flexible material into a flat bag shape. (For example, refer to Patent Document 1).

JP 2002-365299 A

  FIG. 23 is a configuration diagram of the biochip described in Patent Document 1. FIG. 23A is a sectional view, and FIG. 23B is a plan view. The flat blood collection bag 41 whose periphery is sealed has a fish-shaped bag at the center. The opening of the fish-shaped bag is sealed with a rubber stopper 42.

  In the blood collection bag 41, a collection part 43, a pretreatment part 44, a coupling part 45, and a waste liquid storage part 47 are formed in this order from the stopper 42 to the back. At the time of blood collection, the stopper 42 is inserted into a syringe (not shown). An injection needle protrudes inside the syringe and penetrates the stopper 42.

  At the time of blood collection, the test subject is pierced with the needle tip outside the syringe, and the hook 431 of the blood collection bag 41 is pulled outward to collect blood in the collection unit 43. After blood collection, remove the syringe from the blood collection bag. Thereafter, as shown in FIG. 24, the blood collection bag 41 is crushed from the collection unit 43 toward the pretreatment unit 44 with the rotation rollers 61 and 62 interposed therebetween. The collected blood is sent to the preprocessing unit 44.

  When the positions of the rollers 61 and 62 advance and crush the bag portion 48, the solution in the bag portion 48 breaks the valve 49 and flows into the pretreatment portion 44. Next, the solution of the bag part 50 flows into the pretreatment part 44 in the same manner. When the predetermined processing is completed in the preprocessing unit, the roller is rotated to send the processed blood to the coupling unit 45.

  A DNA chip 46 is disposed in the coupling part 45, and hybridization is performed. Excess blood and solution pushed out from the pretreatment unit 44 accumulate in the waste liquid storage unit 47. The state of the hybridized DNA chip is observed by a reading device arranged outside.

However, the conventional method using a beaker or pipette has a problem that the operation is complicated, individual differences are large, and labor is required.
In the case of a blood collection bag, there is a problem that the solution cannot be easily moved due to lack of elasticity.

  In order to solve this problem, there has been an attempt to make a cartridge. Like the biochip described above, a solution is sent to a chamber (hereinafter referred to as a well) that is provided in the cartridge and connected to perform processing such as mixing and chemical reaction. . However, when it is made into a cartridge, there are the following problems.

(1) When the solution is sent to the next well, air is already contained in the well to be sent, and the air is mixed into the solution. Further, the solution is returned by the back pressure of the air.

(2) At the time of liquid feeding, not only the next well but also the solution flows to the wells and flow paths ahead.

(3) The solution flows out to other wells during heating and shaking.

(4) Air is mixed when the sample is first injected. Moreover, since it injects manually, quantitative property is bad (a fixed amount cannot be used as the starting amount of reaction).

(5) Simple mixing of liquid A and liquid B (A + B) is easy, but it is impossible to realize a structure (cross structure) of DNA extraction or purification using silica or magnetic particles from a sample, for example.

  An object of the present invention is to solve the above-described problems and to realize a chemical reaction cartridge having high accuracy and high reproducibility, a manufacturing method thereof, and a chemical reaction cartridge driving mechanism.

  The present invention is a chemical reaction cartridge, a manufacturing method thereof, and a chemical reaction cartridge drive mechanism configured as follows.

(1) At least a part is composed of a container formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A chemical reaction cartridge comprising an exhaust passage for exhausting indoor air into a chamber into which at least the fluid substance is introduced.

(2) It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A chemical reaction cartridge, wherein at least one of the flow path and the chamber has a volume of zero before the fluid substance is introduced.

(3) A method for producing the cartridge according to (2),
A method for manufacturing a cartridge for chemical reaction, wherein a non-adhesive portion that does not adhere the flow path or the chamber portion is formed when the rigid body and the elastic body are bonded.

(4) The method for producing a cartridge for chemical reaction according to (3), wherein the non-adhesive portion is formed by applying a non-adhesive substance before adhesion.

(5) The method for producing a cartridge for chemical reaction according to (3), wherein the non-adhesive portion is formed by applying a material that does not activate the region with a mask or plasma during plasma processing.

(6) The non-adhesive portion is formed by applying an adhesive around a corresponding region and not applying the adhesive to the non-adhesive portion. Production method of cartridge.

(7) The chemistry according to (3), wherein the non-adhesive portion is formed by embedding an embedding material having at least one non-adhesive surface in the substrate to form the non-adhesive surface. A method for producing a reaction cartridge.

(8) The non-adhesive portion is formed by casting a corresponding elastic body or substrate by placing an embedding material having at least one non-adhesive surface on the previously cured substrate or the elastic body. The method for producing a chemical reaction cartridge as described in (3), wherein

(9) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A drive mechanism for a chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A drive mechanism for a chemical reaction cartridge having pressurizing means for simultaneously sealing all input and output flow paths connected to the chamber holding the fluid substance.

(10) The chemical reaction cartridge drive mechanism according to (1) or (2),
A drive mechanism for a chemical reaction cartridge, comprising pressurizing means for simultaneously sealing all the flow paths connected to the chamber holding the fluid substance.

(11) The chemical reaction cartridge drive mechanism according to (9) or (10), wherein the pressurizing means is a roller used for moving a fluid substance.

(12) The chemical reaction cartridge drive mechanism according to (9) or (10), wherein at least one of the pressurizing means is a shutter.

(13) The chemical reaction cartridge drive mechanism according to (9) or (10), wherein the sealing of the fluid substance is simultaneously performed at a plurality of locations in one cartridge.

(14) The chemical reaction cartridge drive mechanism according to (9) or (10), wherein the sealing of the fluid substance is performed at each reaction step.

(15) The chemical reaction cartridge according to (9) or (10), wherein the pressure in the chamber is changed by moving at least one of the pressurizing means after sealing the fluid substance. Drive mechanism.

(16) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber provided with a plurality of inflow channels into which a plurality of different fluid substances flows and at least one discharge channel.
At least one of the chambers is a dummy chamber for adjusting a timing for feeding a predetermined fluid substance to the common chamber.

(17) The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber provided with a plurality of inflow channels into which a plurality of different fluid substances flows and at least one discharge channel.
The chemical reaction according to (1) or (2), wherein at least one of the chambers is a dummy chamber for adjusting a timing for feeding a predetermined fluid substance to the common chamber Cartridge.

(18) The chemical reaction cartridge drive mechanism according to (16) or (17),
A plurality of pressurizing means provided at positions for sealing the flow paths of the plurality of chambers;
A driving mechanism for a chemical reaction cartridge, wherein the plurality of pressurizing means simultaneously move in the same direction and feed liquid one pitch at a time.

(19) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The inlet for the fluid substance is composed of a plurality of injection parts coupled inside,
A cartridge for chemical reaction, characterized in that internal air is discharged from another injection part by injection of a fluid substance from one injection part.

(20) The inlet for the fluid substance is composed of a plurality of injection parts coupled inside,
The chemical reaction cartridge according to (1) or (2), wherein the air inside is discharged from the other injection part by injecting the fluid substance from one injection part.

(21) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
An inlet for storing a predetermined amount of the fluid substance;
Means for sucking a certain amount of fluid substance at the inlet into the interior,
A cartridge for chemical reaction, comprising:

(22) an inlet for storing a predetermined amount of the fluid substance;
Means for sucking a certain amount of fluid substance at the inlet into the interior,
The cartridge for chemical reaction according to (1) or (2), characterized in that it has a cartridge.

(23) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
When one fluidic substance flows into and out of the common chamber, the flow of the other fluidic substance and at least one outlet flow path are sealed by the external force. cartridge.

(24) At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow, and at least two discharge channels,
When one fluid substance flows into and out of the common chamber, the flow of the other fluid substance and at least one flow path through which the fluid substance flows are sealed by the external force (1) Or the cartridge for chemical reactions as described in (2).

(25) The flow path through which the other fluid substance flows in and out is formed at a position sealed by an external force for sending the one fluid substance (23) or (23) 24) The chemical reaction cartridge according to 24).

(26) Each of the plurality of chambers has a convex portion that is pressed down when the external force is applied to the outside of the container, and the flow path for sending the one fluid substance is formed in a concave portion between the convex portions. The cartridge for chemical reaction according to (23) or (24), wherein

(27) The chemical reaction cartridge drive mechanism according to (23) or (24),
The flow path through which the other fluid substances flow in and out is simultaneously sealed by a wheel-type pressurizing means, and the chemical reaction cartridge drive mechanism.

(28) The wheel-type pressurizing means seals the flow path through which the other fluid substances flow in and out by the same axial movement or rotational movement as the pressurizing means for feeding the liquid. The drive mechanism for the chemical reaction cartridge according to (27).

(29) The chemical reaction cartridge drive mechanism according to (23) or (24),
The inflow passage and the outflow passage of the common chamber are formed radially around the common chamber, and the pressurizing means for feeding the respective passages is provided within the surface of the container. The mechanism for driving a chemical reaction cartridge according to claim 1, wherein the mechanism moves in different axial directions in accordance with the respective flow paths.

(30) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
The chemical reaction cartridge, wherein the plurality of flow paths passing through the common chamber are respectively disposed on a back surface and a front surface of the cartridge.

(31) At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow in and at least two discharge channels,
The chemical reaction cartridge according to (1) or (2), wherein the plurality of flow paths that pass through the common chamber are respectively disposed on a back surface and a front surface of the cartridge.
(32) It is composed of a rigid substrate and an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber having flow paths through which two or more different fluid substances flow in and out, respectively.
The flow path of this common chamber is arranged adjacent to the common chamber in a straight line, and when one fluidic substance flows into and out of the common chamber, the other fluidic substance The cartridge for chemical reaction, wherein the inflow channel is sealed by the external force.

(33) At least one of the chambers is a common chamber having flow paths through which two or more different fluid substances flow in and out,
The flow path of this common chamber is arranged adjacent to the common chamber in a straight line, and when one fluidic substance flows into and out of the common chamber, the other fluidic substance The cartridge for chemical reaction according to claim 1 or 2, wherein the inflow channel is sealed by the external force.

(34) The drive mechanism for the chemical reaction cartridge according to (32) or (33),
The pressurizing means for feeding liquid to the common chamber is installed so as to sandwich the chamber with the fluid substance to be fed, and moves in the straight direction in the container surface. Drive mechanism for chemical reaction cartridge.

(35) At least part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
A chemical reaction cartridge, wherein when one fluid substance flows into and out of the common chamber, a flow path into which another fluid substance flows is sealed by the external force.

(36) The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
3. When one fluid substance flows into and out of the common chamber, a flow path into which another fluid substance flows is sealed by the external force. The cartridge for chemical reaction as described.

(37) The chemical reaction cartridge driving mechanism according to (35) or (36),
A mechanism for driving a chemical reaction cartridge, wherein the pressurizing means for feeding different fluid substances are a plurality of pressurizing means groups that move independently from each other.

(38) The chemical reaction cartridge drive mechanism according to (37), wherein the plurality of pressurizing means groups move in at least two different directions within the container surface.

(39) The chemical reaction cartridge driving mechanism according to (38), wherein the two directions are directions different by 90 degrees.

(40) The chemical reaction cartridge according to (35) or (36), wherein the moving direction of the pressurizing means for applying the external force and the direction of the flow path are different in the container surface.

(41) The chemical reaction cartridge according to (40), wherein the direction of the flow path forms an angle of 90 degrees or less with respect to the moving direction of the pressurizing means.

(42) A rigid body is formed on the container side of the flow path, and the entire flow path of the rigid body forming portion is sealed by applying an external force to a part of the flow path (35). , (36), (40) or (41).

(43) A trapping agent for trapping a predetermined substance is permanently or temporarily fixed in the common chamber (16), (17), (23), (24), (25), (26), (30), (31), (32), (33), (35), (36), (40), (41) or the cartridge for chemical reaction according to (42) .

(44) The chemical reaction cartridge according to (43), wherein the predetermined substance is a biopolymer.

(45) The chemical reaction cartridge according to (44), wherein the biopolymer is DNA, RNA, protein, or sugar chain.

(46) The chemical reaction cartridge according to (43), wherein the trapping agent is a bead, a filter or a column whose surface is modified for trapping.

(47) The cartridge for chemical reaction according to (46), wherein the beads are silica, magnetic beads, metal beads, or resin beads.

(48) At least a part of the container is formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
2. The chemical reaction cartridge driving mechanism according to claim 1, wherein the external force is generated by pressing a two-dimensional plate from one direction on the elastic body side.

(49) The chemical reaction cartridge drive mechanism according to (1) or (2),
2. The chemical reaction cartridge driving mechanism according to claim 1, wherein the external force is generated by pressing a two-dimensional plate from one direction on the elastic body side.

(50) The chemical reaction cartridge drive mechanism according to (48) or (49), wherein the plate has a curvature on a surface to which the external force is applied to the flow path or the chamber.

The present invention has the following effects.

According to the first to eighth aspects of the present invention, since the exhaust passage for exhausting the indoor air is provided in the chamber into which the fluid substance is introduced, the chamber is returned by the back pressure of the extruded air. And there is no air in it.
Further, since the flow path and the chamber have a zero volume structure, the same effect is obtained.

According to the ninth to eighteenth aspects of the present invention, since the pressurizing means for simultaneously sealing all the input and output flow paths of the chamber for holding the fluid substance is provided, only the next chamber is supplied during liquid feeding. It can be prevented that it flows to the room ahead. Moreover, the outflow to the other chamber at the time of heating and vibration can be prevented.

According to the nineteenth to twenty-second aspects of the present invention, air of fluid substance can be exhausted at the inlet, so that mixing of air at the time of sample injection can be prevented. Also, a certain amount of sample can be sent into the cartridge.

According to the invention of Claims 23 to 47, at least one of the chambers includes an inflow channel into which two or more different fluid substances flow, and at least two discharge channels. When one fluid substance flows into and out of the common chamber, the flow path through which the other fluid substance flows in and out is sealed by an external force, so that different fluids Common rooms can be crossed.
Accordingly, steps such as extraction and purification of a predetermined substance from the sample can be realized.

According to the 48th to 50th aspects of the present invention, a two-dimensional plate having a curvature on the contact surface with the container is used to press and move the container. Thereby, since a flow path and a chamber are pushed with a surface, the return of the solution and air by back pressure can be prevented.

  Hereinafter, the present invention will be described in detail with reference to the drawings. FIG. 1 is an external view showing an embodiment of a chemical reaction cartridge according to the present invention.

  1A is a perspective view of a cartridge, and FIG. 1B is a plan view of the cartridge. The cartridge 101 is formed of an elastic body 102 such as rubber that is airtight and elastic, and a substrate 103 on a flat plate made of a hard material. As the elastic body 102 of the cartridge, a viscoelastic body or a plastic body can be used. However, in this embodiment, a case where an elastic body is used will be described as an example.

  As a material of the substrate 103, glass, metal, hard resin, or an elastic body can be used. The bonding between the elastic body 102 and the substrate 103 may be adhesion (adhesion (in the case of PDMS (PolyDiMethylSiloxane) and glass) or the like), or welding by ultrasonic waves, heating, plasma treatment, vibration, or the like, in addition to adhesion.

  On the back surface of the elastic body 102, wells A1 to A7 that are holes for storing solutions, flow paths 105a to 105f that connect the wells, intake paths 104a to 104c that send air to the wells A1, A2, and A4, In a state where the common intake passage 104 to which the intake passages are connected, the exhaust passages 106a to 106c for discharging air from the wells A3, A5, and A7, and the common exhaust passage 106 to which the respective exhaust passages are connected are recessed in the upper surface direction. The corresponding region is formed in a convex shape on the upper surface side of the elastic body 102. Flat portions other than the wells, the flow path, the intake path, and the exhaust path on the back surface of the elastic body 102 are bonded to the surface of the substrate 103. As a result, each well, flow path, intake path, and exhaust path are sealed by the elastic body 102 and the substrate 103, so that external leakage of the solution can be prevented.

Next, the operation of transferring the solution in the cartridge thus configured will be described.
FIG. 2 is an explanatory view for explaining liquid feeding and exhausting according to the present invention.
In FIG. 2, the wells B1 and B2 are connected by a flow path 107a, and the opening areas of the flow paths 107a and 107b near the outlets of the wells B1 and B2 are made narrower than the exhaust paths 108a and 108b to form a throttle. (For example, an opening area of about 1/3 to 1/5). As a result, with respect to the discharge of air, the discharge resistance to the flow paths 107a and 107a increases, so that the air flows out to the exhaust paths 108a and 108b. Specifically, it is as follows.

The roller 109 is pressed from above so that the convex part on the surface of the cartridge is crushed. In this state, when rotating in the direction of the solid arrow and moving to the right, the solution in the well B1 is pushed rightward. As a result, the solution flows into the well B2 through the channel 107a. At this time, the air 110 in the well B2 is pushed by the inflow of the solution and exhausted through the exhaust path 108b as indicated by the broken line arrow.

Further, since the exhaust path on the liquid supply side is blocked by the roller 109, the solution does not leak to the exhaust path side, and the residual liquid in the exhaust path 108a is sent to the next well B2 by the movement of the roller 109.
As described above, it is possible to realize a cartridge in which air does not mix with the solution without returning due to the back pressure of the extruded solution or air.

FIG. 3 is an explanatory view showing another embodiment (zero volume structure) of the chemical reaction cartridge according to the present invention.
FIG. 3A is a plan view of the cartridge. As shown in FIG. 3B, the cartridge 111 has an airtight and elastic elastic body 117 and a flat plate as in the previous embodiment. The substrate 118 is formed. These can be produced by, for example, PDMS (PolyDiMethylSiloxane). On the back surface of the elastic body 117, a well C1 that is a hole in which the solution is accumulated and flow paths 112 and 113 for allowing the solution to flow into the well C1 are provided.

In addition to the well C1, wells C2 and C3 are provided, the well C2 is connected to the well C1 through the flow path 114, and the well C3 is connected to the well C2 through the flow path 115. Here, the flow paths 114 and 115 and the wells C2 and C3 are brought into close contact with the elastic body 117 and the substrate 118 without bonding, so that the volume before the solution is introduced or after the solution passes is zero. It is trying to become. By doing so, air is eliminated from the flow path and the well, so that air venting is unnecessary.

As can be seen, the well C1 and the flow paths 112 and 113 shown by solid lines in FIG. 3A protrude from the surface of the cartridge 111 in a convex shape and can be seen, but the flow paths 114 and 115 shown by broken lines and Wells C2 and C3 are not visible.

The operation of such a cartridge during liquid feeding is as follows.
As shown in FIG. 3A, the roller 116 is pressed from above so that the surface of the cartridge 111 (the flow paths 112 and 113 and the well C1) is crushed. When the roller 116 is rotated in the direction indicated by the arrow and moved to the right, the solution accumulated in the well C1 also moves and flows into the well C2 through the flow path 114. At this time, the flow path 114 and the well C2 having a volume of zero have a portion of the elastic body 117 facing the substrate 118 of the flow path 114 and the well C2 due to the inflow of the solution as shown in FIG. The solution is pushed up to form a solution passage (flow path 114) and a reservoir (well C2). After the solution passes, the volume becomes zero again by the restoring force of the elastic body 117.

Similarly, in the channel 115 and the well C3, as shown in FIG. 3D, the solution flows from the well C2 through the channel 115 to the well C3 by the movement of the roller 116. The volume of the flow path 115 and the well C3 is zero before the solution flows in, and a passage (flow path 115) and a reservoir (well C3) are formed as the solution flows. Such a structure is possible because the container is the elastic body 117.

Next, an embodiment of a method for producing such a cartridge having a zero volume structure will be described.
FIG. 4 is an explanatory view for explaining a first embodiment of a cartridge manufacturing method. The steps of the cartridge manufacturing method will be described below with reference to FIG.

(1) A mask 119 and a substrate 120 are prepared (FIG. 4A).

(2) Plasma treatment is performed with the mask 119 placed on the substrate 120 (FIG. 4B).
As a result, the portion other than the mask 119 (shaded portion) is subjected to plasma treatment, and only that portion can be bonded (FIG. 4C).

(3) The mask 119 is removed, and the substrate 120 and an elastic body (not shown) are bonded.
Instead of the mask 119, a substance that is not activated by plasma may be applied to the non-bonded portion 121 of the substrate 120 before the plasma treatment.
In addition, the plasma processing related to PDMS is a well-known technique (for example, Plasma Material Science Handbook, Ohmsha, 1992), and thus the description thereof is omitted.

FIG. 5 is an explanatory view for explaining a second embodiment of the cartridge manufacturing method. The steps of the cartridge manufacturing method will be described below with reference to FIG.

(1) A notch is provided around the non-bonding portion 125 of the substrate 122, and the adhesive 124 is applied so as to fill the portion. The notch may be cut off except for the non-adhered part as shown in FIG. 5 (a), or may be cut around the non-adhered part as shown in FIG. 5 (b).

(2) The substrate 122 and the elastic body 123 are bonded.
Note that the notch is provided in the substrate 122 in order to prevent the adhesive 124 from flowing out to the non-adhesive portion. If a non-adhesive substance is applied to the non-adhesive portion 125 before bonding, the substrate 122 is provided. It is not necessary to provide a notch in.

  FIG. 6 is an explanatory view for explaining a third embodiment of the cartridge manufacturing method. The steps of the cartridge manufacturing method will be described below with reference to FIG.

(1) The embedding material 128 having a non-adhesive surface is placed on the non-adhesive portion 129 of the elastic body 127.

(2) The material of the substrate 126 is poured and cured from above (for example, casting molding).

As a result, the elastic body 127 and the substrate 126 are bonded at a portion other than the embedding material 128.
Note that the embedding material 128 can also be produced by PDMS, for example.

Next, the structure of the cartridge for chemical reaction and the drive mechanism for performing liquid feeding in the cartridge will be described.
FIG. 7 is an explanatory view showing a first embodiment relating to the cartridge and the drive mechanism.
In FIG. 7A, the roller 130a is pressed against the cartridge and shields the flow path 131a, which is the path for the solution in the well D1. The roller 130b shields the flow path 131b that is the exit path. In this way, all the inlet / outlet paths of the well are simultaneously shielded by a plurality of rollers, thereby preventing the solution from flowing not only to the next well but also to the flow path and well ahead of the next well. Can do.

  Further, as shown in FIG. 7B, the vicinity of the solution outlet in the well D1 is shielded by a roller 130b and locked so that the roller 130b does not move. In this state, when the roller 130a is rotated in the direction of the arrow so as to sandwich the well D1, the solution in the well D1 can be pressurized.

  Further, as shown in FIG. 7 (c), the vicinity of the solution inlet of the well D1 is shielded by a roller 130a and locked so as not to move. When the roller 130b is rotated and moved away from the well D1, the solution in the well D1 can be depressurized.

FIG. 8 is an explanatory view showing a second embodiment relating to the cartridge and the drive mechanism.
FIG. 8A, FIG. 8B, and FIG. 8C show how the state changes as the rollers 132a, 132b, and 132c move in the direction of the arrows. The shaded area indicates the presence of the solution. In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage.

In FIG. 8 (a), the wells E1 and E2 are provided with flow paths 133a and 133b, respectively, which are solution paths, the wells E1 and E3 are connected by a flow path 133c, and the wells E2 and E3 are connected to the flow path 133d. It is connected with. The well E3 is provided with a flow path 133e that is a solution outlet. Here, the wells E1, E2 are aligned so that liquid can be fed simultaneously by one roller.

The roller 132a shields the flow paths 133a and 133b, and the roller 132b shields the flow paths 133c and 133d. This prevents the solution in the wells E1 and E2 from flowing into the well E3.

FIG. 8B shows a state in which the rollers 132a, 132b, and 132c move, the roller 132a is positioned on the wells E1 and E2, and the roller 132b is positioned on the well E3. The solutions in the wells E1 and E2 are pushed out by the roller 132a and are in the well and the flow path between the roller 132a and the roller 132b, as indicated by the oblique lines.

In FIG. 8C, the rollers 132a, 132b, and 132c (132c not shown) further move, the roller 132a is positioned on the flow paths 133c and 133d, and the roller 132b is positioned on the flow path 133e. Is shown. As indicated by the oblique lines, all the solutions in the wells E1 and E2 are pushed out by the roller 132a and moved to the well E3.

The solution in the well E3 does not flow backward due to the shielding of the flow paths 133c and 133d of the roller 132a, and does not flow to the next well (not shown) due to the shielding of the flow path 133e of the roller 132b.

In addition, since the solution entrance and exit passages are shielded from the well, the solution stored in the well can be prevented from flowing out of the well even when heating or vibration is applied from outside the cartridge. .

In this embodiment, the case where the solution in two wells is moved to one well and mixed is shown, but it can be applied to the liquid feeding from one well to one well, and the solution to be mixed is naturally applicable. It is possible to mix two or more types of solutions by providing two or more wells for storing.

Further, as shown in FIG. 8D, the present invention can also be applied to a case where a solution in one well is divided into two wells. In FIG. 8 (d), the operation of each roller is the same as that shown in FIGS. 8 (a), (b), and (c).

In FIG. 8D, the solution in the well E4 is pushed out by the movement of the roller 132e, and is divided into the wells E5 and E6 through the flow paths 133f and 133g. Since the flow paths 133f and 133g are shielded by the roller 132e and the flow paths 133h and 133i are shielded by the roller 132f, the solution does not flow out from the wells E5 and E6.

  Furthermore, in this embodiment, the outflow of the solution is shielded by a roller that moves parallel to the front side of the cartridge, but the shielding of the flow path is a shutter that moves perpendicularly to the front side of the cartridge and shields the flow path. Such shielding means may be used.

  FIG. 9 is an explanatory view showing a third embodiment relating to the cartridge and the drive mechanism. In this example, the extraction of biopolymers such as DNA (deoxyribonucleic acid), RNA (ribonucleic acid), protein, and sugar chain will be described as an example.

  In FIG. 9, the wells F1 to F13 provided in the cartridge have the well F9 as a common well, the wells F6 to F8 are connected to the well F9, and are arranged in the left vertical column of the well F9. Further, the well F6 is used as a common well, and the wells F1 and F2 are connected to the well F6 and arranged in one column on the left. Wells F3 and F5 arranged in the same vertical direction are arranged. Well F3 is connected to well F7, and well F5 is connected to well F8. A well F4 is connected to the well F5 and is located on the left side of the well F4.

  The well F9 is connected to the well F10 and is located to the right of the well 9, and the well F11 to the well 13 are connected to the cascade in a horizontal row.

These wells are arranged at equal intervals in the lateral direction (rolling direction of the roller), and a plurality of rollers (for convenience, only reference numerals 134a, 134b, and 134c are provided) are arranged at intervals in the lateral direction of the wells. Arranged at matching intervals.

In addition, it has shown that the pattern shown to the well is the same content.
In this embodiment, the well structure is a zero volume structure, but a structure having an exhaust path may be used. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

  FIG. 9A shows a state where each well entry / exit path and the position of the roller are set to coincide. Well F1 contains a sample solution, well F2 contains a solution, well F3 contains a DNA trapping agent (surface-modified magnetic particles), and well F4 contains a washing solution. The other wells are in a zero volume state.

  Each roller rotates in the direction of the solid arrow. The rollers 134a, 134b, and 134c hold the entrance / exit from the wells F1 to F5 to prevent the fluid such as the sample solution from flowing out.

  FIG. 9B shows a state in which each roller has rotated and moved in the direction of the arrow by one well. Accordingly, the sample solution in the well F1 and the solution in the well F2 are mixed in the well F6 by the movement of the roller 134b, and the trapping agent in the well F3 moves to the well F7.

Further, the cleaning liquid in the well F4 moves to the well F5 by the movement of the roller 134a. Here, the wells F7, F5, and F8 are originally empty wells, and are dummy wells for adjusting the timing at which the trap agent and the cleaning liquid are fed to the well F9. Because of this, liquid can be fed to the target well at an arbitrary timing by only moving the roller on one axis.
In the well F6, a process of heating and reacting the mixed solution is added. For heating, for example, a Peltier element is used.
Note that the dummy well has the same volume as the well in which the solution or the like was initially held.

FIG. 9C shows a state in which each roller rotates and moves in the direction of the arrow by one well from the state of FIG. 9B. Therefore, the movement of the roller 134b causes the well F6 mixture and the F7 DNA trapping agent to be mixed in the well F9. Further, the cleaning liquid in the well F5 moves to the well F8 by the movement of the roller 134a.
In the well F9, DNA is trapped in the DNA trapping agent, and the magnetic particles that are the trapping agent are trapped in the well F9 by applying a magnetic field.

  FIG. 9D shows a state in which each roller rotates and moves in the direction of the arrow by one well from the state of FIG. 9C. Accordingly, the waste liquid after the DNA trap in the well F9 moves to the well F10 by the movement of the roller 134b. Further, the cleaning liquid in the well F8 moves to the well F9 by the movement of the roller 134a. In the well F9, the magnetic particles are washed with the washing liquid.

  FIG. 9 (e) shows a state in which each roller has rotated and moved in the direction of the arrow by one well from the state of FIG. 9 (d). Therefore, the waste liquid in the well F10 moves to the well F11 by the movement of the roller 134b. Further, the cleaning liquid after cleaning the well F9 moves to the well F10 by the movement of the roller 134a.

As described above, the DNA trapped in the magnetic particles remains in the well F9, so that the DNA can be extracted.
Note that beads, filters, columns, or the like are used for trapping DNA. The beads are silica, magnetic beads, metal beads or resin beads.
The liquid feeding mechanism described here is similar to the movement of the shift register of the digital circuit, and such a liquid feeding structure can be said to be a clock-type liquid feeding structure. The difference from the electric system is that the flow paths are independent because it is necessary to prevent contamination (mixing) between the solution and the cleaning solution.

FIG. 10 is an explanatory view showing a fourth embodiment according to the cartridge and the drive mechanism.
FIG. 10 shows the solution inlet portion of the cartridge 135. In FIG. 10A, the introduction port 137 is like a U-shaped passage that passes through the inside of the cartridge 135 from the outside of the cartridge 135 and communicates with the outside of the container again. The U-shaped inlet 137 is connected to a processing flow path 138 inside the cartridge 135. The introduction port 137 has a predetermined volume regardless of the presence or absence of a solution, and the flow path 138 is the above-described zero volume structure flow path.

The solution is injected by a syringe 136 into one injection portion 140a of the introduction port 137. The air at the introduction port 137 from the beginning is pushed by the solution and discharged outside from the other injection part 140b as shown by the solid arrow.
Next, as shown in FIG. 10 (b), the rollers 139a and 139b are pressed against the cartridge 135 from the upper surface of the drawing, so that the injection portions 140a and 140b are simultaneously closed by the roller 139a, and the flow path 138 is shielded by the roller 139b. Keep it. These two rollers rotate and move in the direction of the broken line arrow to push the solution in the inlet 137 into the flow path 138 as indicated by the solid line arrow.

As described above, air can be prevented from being mixed into the solution. Also, a certain amount of the U-shaped portion can be sent into the cartridge.
Further, as shown in FIG. 10C, the shape of the cartridge surface of the inlet 137 is easily blocked by the roller 139a by applying a taper process tapered toward the inlet, thereby preventing air from entering. Can do.

FIG. 11 is an explanatory view showing a fifth embodiment according to the cartridge and the drive mechanism.
FIG. 11 shows the inlet portion of the cartridge. In FIG. 11A, the inlet 141 is connected to a dome-shaped well G1 through a flow path 144a, and a flow path 144b for sending the solution into the cartridge is connected to the well G1.

In this embodiment, the structure of the flow path is a zero volume structure, but a structure having an exhaust path may be used. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

  The roller 143a moves in advance from the right to the left on the well G1, and discharges the air in the dome from the inlet 141 side. The roller 143a presses and seals the flow path 144a, and in this state, a large amount of the sample solution 142 is injected into the inlet 141. Next, when the roller 143a moves in the direction of the arrow, the well G1 is crushed. Since the well G1 tries to return to the original state by passing the roller 143a, the solution 142 is sucked into the well G1. Thereby, only a fixed amount of the solution is sucked. When the roller 144a passes over the well G1 and presses and seals the flow path 144b, the flow path 144a is pressed and sealed by the roller 143b.

As described above, the portion of the solution mixed with air remains in the inlet 141, so that the air is not involved in the solution. Also, a certain amount can be used as a starting amount for the reaction.
The introduction port may be used in combination with the U-shaped introduction port described above. Further, the structure of the cartridge surface of the introduction port may be tapered as in the above U shape.

FIG. 12 is an explanatory view showing a sixth embodiment according to the cartridge and the drive mechanism.
In this example, extraction of biopolymers such as DNA, RNA, protein, sugar chain and the like will be described as an example.
In FIG. 12, a well H1 is a well in which a sample such as blood and a solution of a lysis solution are placed. The well H2 is a well containing a DNA trapping agent such as silica or amino magnetic particles (surface-modified magnetic particles). The well H3 is a waste stop well. The well H4 is a well containing an extraction solvent. Well H5 is a well into which a DNA extract is placed.

  These wells are in a cross arrangement in which at least two different types of solutions flow from different channels into a common well having at least two discharge channels. A cartridge having a cross structure in which other wells are radially arranged around the common well and connected by a flow path is driven in the following process.

(1) Trap process
The DNA contained in the well H1 is negatively charged. Silica and amino magnetic particles installed in the well H2 are positively charged. For this reason, when liquid is fed from the well H1 to the well H3, the DNA is trapped (captured) in the well H2. The remaining liquid is sent to the well H3 as waste liquid.

(2) Release process After the trap process, the extraction solvent in the well H4 is sent to the well H2 and the pH and temperature are adjusted, so that the DNA is released from the trap agent. This is sent to the well H5 to obtain a DNA extract.

  In such a process, the liquid feeding from the well H1 to the H3 should not be sent to the wells H4 and H5. Similarly, in the liquid feeding from the well H4 to the H5, the liquid should not be sent to the wells H1 and H3.

  For this reason, when the liquid is fed from the well H1 to the H3, as shown in FIG. 12A, the flow path to the wells H4 and H5 is blocked by the shutters 145a and 145b. Further, when liquid is fed from well H4 to H5, as shown in FIG. 12B, the flow path to wells H1 and H3 is blocked by shutters 145c and 145d. The shutter may be a liquid feeding roller.

In this embodiment, the structure of the well and the flow path is a zero volume structure, but a structure having an exhaust path may be used. In addition, although a roller (not shown) is used as the pressurizing means for feeding the liquid, a piston type actuator may be used.
Furthermore, beads, filters, columns, or the like are used for the magnetic particles. The beads are silica, magnetic beads, metal beads or resin beads.
As described above, for example, nucleic acid extraction from samples using silica or magnetic particles, and purification after PCR (Polymerase Chain Reaction) amplification (separation of unreacted substances and products) (cross structure) is realized. can do.

FIG. 13 is an explanatory view showing a seventh embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes the trap process and release process described above.
In FIG. 13, a well I1 is a well in which a sample such as blood and a solution of a lysis solution are placed. The well I2 is a well containing a DNA trapping agent such as silica or amino magnetic particles (surface-modified magnetic particles). The well I3 is a waste stop well. Well I4 is a well containing an extraction solvent. Well I5 is a well into which a DNA extract is placed. The wells I1, I3, I4, and I5 are connected to the well I2 through a flow path, and a roller that applies an external force feeds a specific well or flow path and does not feed liquid at the same time. Is arranged to seal. Such wells have a cross arrangement in which at least two different types of different solutions flow from different channels into a common well having at least two discharge channels.

  Specifically, as shown in FIG. 13A, the roller 146a rotates in the direction of the arrow to push out the solution in the well I1 and send it to the well I2. The hatched portion indicates the path through which the solution is sent. At this time, the roller 146b seals the flow path connecting the well I2 and the wells I4 and I5. Therefore, the solution sent to the well I2 is sent to the well I3 as a waste liquid after the biopolymer in the sample is trapped in the well I2.

Further, when the rollers 146a and 146b move and reach the position shown in FIG. 13B, the roller 146a pushes out the extraction solvent in the well I3 and simultaneously seals the flow path connecting the well I2 and the wells I1 and I3. In order to stop, the extraction solvent in the well I4 is sent to the well I2, the DNA is released from the trapping agent in the well I2, and the DNA extract is sent to the well I5.
In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

  As described above, a simple structure, for example, extraction of nucleic acid from a sample using silica or magnetic particles, and purification (separation of unreacted substances and products) after PCR amplification (cross structure) is realized. can do.

FIG. 14 is an explanatory view showing an eighth embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes the biopolymer trap process and release process described above.
In FIG. 14, a well J1 is a well in which a sample such as blood and a solution of a lysis solution are placed. The well J2 is a well containing a DNA trapping agent such as silica or amino magnetic particles (surface-modified magnetic particles). The well J3 is a waste stop well. Well J4 is a well containing an extraction solvent. Well J5 is a well into which a DNA extract is placed. The wells J1, J3, J4, and J5 are connected to the well J2 through a flow path, and one of the rollers for applying an external force feeds a specific well or flow path, while another roller feeds it. It arrange | positions so that the flow path which does not perform a liquid may be sealed. Such wells have a cross arrangement in which at least two different types of different solutions flow from different channels into a common well having at least two discharge channels.

Each well has convex portions 176a to 176h where the roller for applying an external force from outside the container of the cartridge contacts, and the flow path connecting the two wells is formed in a concave portion between the two convex portions. .
Note that the convex portion may be on either the cartridge or the roller, and may be any structure that does not seal across the flow path when two wells are pressed by the roller.
In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

  Specifically, as shown in FIG. 14A, the roller 147a rotates and moves in the direction of the arrow while pressing the convex portions 176a and 176b to push out the solution in the well J1 and send it to the well J2. The flow path has an S shape, and the hatched portion indicates the path through which the solution is sent. At this time, the roller 147b seals the flow path connecting the well J2 and the wells J4 and J5 below the convex portion 176c. Therefore, the sample solution sent to the well J2 is sent to the well J3 as a waste liquid after the biopolymer in the sample is trapped in the well J2.

  Further, when the rollers 147a and 147b move to the position shown in FIG. 14B, the roller 147b pushes out the extraction solvent in the well J4. At this time, the roller 147a seals the flow path connecting the well J2 and the wells J1 and J3 below the convex portion 176a, so that the extraction solvent in the well J4 is sent to the well J2, and the DNA is trapped in the well J2. The DNA extract is sent to the well J5.

In addition, as shown in FIG. 14C, a path that goes around the outside of the well may be used instead of the S-shaped path through the flow path between the wells.
As described above, for example, it is possible to realize a structure (cross structure) of nucleic acid extraction from a sample using silica, magnetic particles, or the like, or purification after PCR amplification (separation of unreacted substances and products).

FIG. 15 is an explanatory view showing a ninth embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes the biopolymer trap process and release process described above.
In FIG. 15, a well K1 is a well in which a sample such as blood and a solution of a lysis solution are placed. The well K2 is a well containing a DNA trapping agent such as silica or amino magnetic particles (surface-modified magnetic particles). The well K3 is a waste stop well. The well K4 is a well containing an extraction solvent. Well K5 is a well into which a DNA extract is placed.

In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.
The wells K1, K3, K4, and K5 are connected to the well K2 via a flow path, and at least two different types of solutions flow from different flow paths into a common well having at least two discharge flow paths. The arrangement is such that the input side and the output side to the common well of the flow path other than the flow path passing through the common well are simultaneously sealed with the same roller.

  Specifically, as shown in FIG. 15A, the roller 148a rotates in the direction of the arrow to push out the solution in the well K1 and send it to the well K2. The hatched portion indicates the path through which the solution is sent. At this time, the roller 148c is a wheel-type structure that pressurizes at both ends, and seals the flow path that connects the wells K4 and K5 across the well K2. Therefore, the sample solution sent to the well K2 is sent to the well K3 as a waste liquid after the biopolymer in the sample is trapped in the well K2.

  Next, the roller 148c moves in the reverse direction on the same axis (X axis) as the rollers 148a and 148b, and seals the flow path connecting the well K2 and the wells K1 and K3. Further, when the rollers 148a and 148b move and reach the position shown in FIG. 15B, the extraction solvent in the roller 148a and the well K4 is pushed out. Therefore, the extraction solvent in the well K4 is sent to the well K2, the DNA is released from the trapping agent in the well K2, and the DNA extract is sent to the well K5.

Here, when the flow path to be fed is not parallel as shown in FIGS. 15A and 15B, but the roller 148c is not translated, the roller 148c rotates as shown in FIG. 15C. Thus, the channel to be sealed may be changed.
As described above, for example, it is possible to realize a structure (cross structure) of nucleic acid extraction from a sample using silica, magnetic particles, or the like, or purification after PCR amplification (separation of unreacted substances and products).

FIG. 16 is an explanatory diagram showing a tenth embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes the biopolymer trapping and releasing processes described above.
In FIG. 16, a well L1 is a well in which a sample such as blood and a solution of a lysis solution are placed. The well L2 is a well containing a DNA trapping agent such as silica or amino magnetic particles (surface-modified magnetic particles). The well L3 is a waste stop well. The well L4 is a well containing an extraction solvent. Well L5 is a well into which a DNA extract is placed.

  In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

The wells L1, L3, L4, and L5 are connected to the well L2 via a flow path, and at least two or more different solutions flow into the common well having at least two discharge flow paths from different flow paths. The arrangement is such that the input side and the output side to the common well of the flow path other than the flow path passing through the common well are simultaneously sealed with the same roller. This is a cross structure in which other wells are radially arranged around the common well and connected by a flow path.

In addition, the rollers for feeding the liquids to the respective flow paths move in different axial directions such as vertical (Y) and horizontal (X) within the surface of the cartridge.
Specifically, as shown in FIG. 16A, the rollers 149a and 149b rotate and move in the direction of the arrow to push out the solution in the well L1 and send it to the well L2. The hatched portion indicates the path through which the solution is sent. At this time, the roller 149c seals the flow path connecting the well L2 and the well L4, and the roller 149d seals the flow path connecting the well L2 and the well L5. Therefore, the sample solution sent to the well L2 is sent to the well L3 as a waste liquid after the biopolymer in the sample is trapped in the well L2.

  Next, the roller 149c moves to the vicinity of the entrance of the well L4 (not shown) in order to push out the extraction solvent in the well L4. The rollers 149a and 149b move on the flow path to seal the flow path connecting the well L1 and the well L2 and the flow path connecting the well L3 and the well L2.

When the rollers 149c and 149d move in the direction of the arrow as shown in FIG. 16 (b), the extraction solvent in the well L4 is sent to the well L2, and the DNA is released from the trapping agent in the well L2, and the DNA extract is extracted. Is sent to well L5. At this time, the roller 149c moves away from the cartridge surface once if necessary.
Further, as shown in FIG. 16 (c), the solution sent from the well L6 to the wells L4 and L7 by the rollers 149e and 149f may be configured in multiple stages so as to be sent from the well L4 to the well L2 by the rollers 149c and 149d. .

Further, as shown in FIG. 16D, the intersecting flow paths may be three or more flow paths, and the angles formed by the respective flow paths may not be a right angle.
In addition, in this embodiment, when the rollers collide with each other, one of them may be provided on the opposite side (the back surface of the cartridge) as shown in FIG.
In FIG. 16 (e), the cartridge 150 is formed of elastic bodies 151a, 151b such as rubber which is airtight and elastic, and a substrate 152 on a flat plate made of a hard material. The substrate 152 is sandwiched and bonded between elastic bodies 151a and 151b, and flow paths 156a and 156b are provided between both elastic bodies and the substrate. These channels are channels that pass through the common well described above.

The substrate 152 is provided with a through hole 153 to connect the flow path 156a and the flow path 156b.
The roller 149m is provided on the surface 154 side of the cartridge 150 and feeds the flow path 156a. The roller 149n is provided on the back surface 155 side of the cartridge 150 and feeds the flow path 156b. Therefore, the rollers 149m and 149n do not collide.

In addition, as the elastic bodies 151a and 151b of the cartridge, viscoelastic bodies or plastic bodies can be used.
Further, as a material of the substrate 152, glass, metal, hard resin, or an elastic body can be used. The bonding between the elastic bodies 151a and 151b and the substrate 152 may be adhesion, adhesion (such as in the case of PDMS and glass), or welding by ultrasonic waves, heating, plasma treatment, vibration, or the like.

  As described above, for example, it is possible to realize a structure (cross structure) of nucleic acid extraction from a sample using silica or magnetic particles, or purification after PCR amplification (separation of unreacted substances and products).

FIG. 17 is an explanatory view showing an eleventh embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes a biopolymer trapping process and a releasing process. In this example, DNA extraction will be described as an example.

In FIG. 17, a well M1 is a well in which a sample such as blood and a sample 158 that is a solution of a lysate are placed. The well M2 is a well containing a DNA trapping agent 159 such as silica or amino magnetic particles (surface-modified magnetic particles), and the trapping agent is fixed in the well M2 by the magnetic force of an external magnet. The well M3 is a well containing the extraction buffer solution 160. The wells M1 and M3 are connected to the well M2 through a flow path, and one of the rollers for applying an external force feeds a specific well or flow path, and at the same time, the other rollers do not feed the liquid. It arrange | positions so that a flow path may be sealed. In such a well, both the flow path for liquid feeding and the flow path for sealing pass through the same common well, and the plurality of flow paths passing through the common well are in a straight line centered on the common well. A plurality of pressurizing means that are arranged adjacent to each other and feed the respective flow paths move in a straight direction in which the flow paths are arranged in the plane of the cartridge.

Although not shown, the well M1 is provided with a flow path for injecting a sample, and the well M3 is provided with a flow path serving as an outlet for the extracted product.
Specifically, the state changes from FIG. 17 (a) to FIG. 17 (g). As shown in FIG. 17A, the roller 157a seals the inlet of the well M1, the roller 157b seals the flow path connecting the well M1 and the well M2, and the roller 157c is the outlet of the well M3. Is sealed.

The rollers 157a and 157b rotate and move in the arrow direction. The roller 157a pushes out the sample 158 in the well M1, and then seals the flow path connecting the well M1 and the well M2. The roller 157b moves from above the flow path connecting the well M1 and the well M2, and seals the flow path connecting the well M2 and the well M3. As a result, the sample 158 in the well M1 is sent to the well M2.

Next, as shown in FIG. 17B, the roller 157a moves in the arrow direction (reverse direction) and returns to the original position. When this reciprocating movement is repeated, the sample 158 and the trapping agent are efficiently mixed in the well M2, and the supplement of DNA to the magnetic particles is completed. When the trapping process is completed, the roller 157a returns to the initial position, and the roller 157b seals the flow path connecting the well M2 and the well M3. (Fig. 17 (c))

Next, as shown in FIG. 17D, the rollers 157b and 157c move in the direction of the arrow. The roller 157b returns to the original position (position shown in FIG. 17A) while pushing the well M2, and removes the sample 158 slightly remaining in the well M2. The roller 157c pushes out the extraction buffer solution 160 in the well M3, sends it to the well M2, and seals the flow path connecting the well M2 and the well M3 (FIG. 17E). In this state, the well M2 is held and a DNA separation process is performed.

When the DNA separation process is completed, the rollers 157b and 157c move in the direction of the arrow (in the opposite direction to the figure (17 (d)) as shown in Fig. 17 (f). After the extrusion, the flow path connecting the well M2 and the well M3 is sealed, and the roller 157c moves from the flow path connecting the well M2 and the well M3 and returns to the initial position (FIG. 17G). Thereby, the extracted product is sent to the well M3 to complete the DNA extraction process.

FIG. 18 is an explanatory view showing a twelfth embodiment relating to the cartridge and the drive mechanism.
This embodiment also realizes a biopolymer trapping process and a releasing process. In this example, as in the eleventh example, DNA extraction will be described as an example.

In FIG. 18, a well N1 is a well into which a sample such as blood and a sample 162 that is a solution of a lysis solution are placed. The well N2 is a well containing a DNA trapping agent 163 such as silica or amino magnetic particles (surface-modified magnetic particles), and the trapping agent is fixed in the well N2 by the magnetic force of an external magnet. The well N3 is a well containing the extraction buffer solution 164. The wells N1 and N3 are connected to the well N2 through a flow path, and one of the rollers for applying an external force feeds a specific well or the flow path, and the other roller does not feed the liquid. It arrange | positions so that a flow path may be sealed. In such wells, both the flow path for liquid feeding and the flow path for sealing pass through the same common well, and the plurality of flow paths passing through the common well are aligned in a straight line with the common well as the center. The plurality of pressurizing means that are arranged adjacent to each other and feed the respective flow paths move in a straight direction in which the flow paths are arranged in the plane of the cartridge.
Although not shown, the well N1 is provided with a flow path for injecting a sample, and the well N3 is provided with a flow path serving as an outlet for the extracted product.

Specifically, the state changes from FIG. 18 (a) to FIG. 17 (f). As shown in FIG. 18A, the roller 161a seals the inlet of the well N1, the roller 161b seals the flow path connecting the well N1 and the well N2, and the roller 161c is the outlet of the well N3. Is sealed.

The rollers 161a and 161b rotate and move in the arrow direction. The roller 161a pushes out the sample 162 in the well N1, and then seals the flow path connecting the well N1 and the well N2. The roller 161b moves from above the flow path connecting the well N1 and the well N2, and seals the flow path connecting the well N2 and the well N3. As a result, the sample 162 in the well N1 is sent to the well N2.

Next, as shown in FIG. 18B, the rollers 161a and 161b move in the arrow direction (reverse direction) and return to their original positions. When this reciprocation is repeated, the sample 162 and the trapping agent 163 are efficiently mixed in the well N2, and the supplement of the DNA to the magnetic particles is completed. When the trapping process is completed, the rollers 161a and 161b return to the initial positions (FIG. 18C). Thereby, the sample remaining in the well N2 is also removed and in a dry state.

This time, as shown in FIG. 18C, the roller 161c moves in the direction of the arrow. The roller 161c pushes the DNA buffer solution 164 in the well N3, sends it to the well N2, and seals the flow path connecting the well N2 and the well N3 (FIG. 17 (d)). In this state, the well N2 is held and the DNA is separated from the magnetic particles.

When the processing is completed, the rollers 161b and 161c move in the direction of the arrow (in the opposite direction to the figure (18 (c)), as shown in Fig. 18 (e), and the roller 161b pushed out the extract of the well N2. Thereafter, the flow path connecting the well N2 and the well N3 is sealed, and the roller 161c moves from the flow path connecting the well N2 and the well N3 and returns to the initial position (FIG. 17 (f)). As a result, the extraction product is sent to the well N3 to complete the DNA extraction process.

FIG. 19 is an explanatory view showing a thirteenth embodiment relating to the cartridge and the drive mechanism. In this example, extraction of biopolymers such as DNA, RNA, protein, sugar chain and the like will be described as an example.
In FIG. 19, wells O1 to O23 provided in the cartridge are provided with wells O1 to O14 in the upper stage P1, the well O10 is a common well, and wells O7 to O9 are connected to the well O10. Arranged in one row. Further, the well O7 is used as a common well, and wells O1 and O2 are connected to the well and arranged in a left vertical column. Wells O3 and O6 arranged in the same vertical direction are arranged. Well O3 is connected to well O8, and well O6 is connected to well O9. The well O6 is connected to the well O5 and the well O4 in a cascade and is located in the left one column.

A well O11 is connected to the well O10, and is located on the right side of the well O10. A well O12 to a well O14 are connected to the well O10 in cascade.
These wells are arranged at equal intervals in the horizontal direction (rolling direction of the roller), and the plurality of hatched rollers are arranged at intervals corresponding to the intervals in the well horizontal direction.
In the lower stage P2, the wells O15 to O23 are arranged at equal intervals in a horizontal row so that the vertical positions thereof coincide with the upper stage wells. The well O19 and the well O20 are connected to the well O10, and the well O19 is connected to the well O7. Wells O20 are arranged in a row, and wells O20 are arranged in a row of wells O11. The wells O19 to O15 are connected to the left side of the wells O10 and cascaded, and the wells O20 to O23 are cascaded to the right side. Are connected to each other.

The plurality of rollers in the upper stage P1 and the lower stage P2 are arranged at intervals corresponding to the intervals in the well lateral direction and seal the flow paths connecting the wells.
In this embodiment, the well structure is a zero volume structure, but a structure having an exhaust path may be used. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.

In FIG. 19A, the well O1 is the sample solution, the well O2 is the solution, the well O3 is the DNA trapping agent (surface-modified magnetic particles), the wells O4 and O5 are the cleaning solution, and the well O15 is the extraction solution. Pre-filled buffer solution. The other wells are in a zero volume state.
Each roller of the upper stage P1 of FIG. 19A rotates in the direction of the solid line arrow. The movement of the contents in the well by this operation is the same as that described in Example 9 above, and will be briefly described below. However, since the cleaning liquid is contained in the wells O4 and O5, the well O10 is cleaned twice.

In the upper stage P1, when the rollers rotate and move in the direction of the arrow for one well, the sample solution in the well O1 and the solution in the well O2 are mixed in the well O7, and the trapping agent in the well O3 is transferred to the well F8. The cleaning liquid in the wells O4 and O5 moves to the wells O5 and O6.
In the well O7, a process of heating and reacting the mixed solution is added. For heating, for example, a Peltier element is used.
When each roller further rotates and moves in the direction of the arrow for one well, the mixed solution in well O7 and the DNA trapping agent in well O8 are mixed in well O10. Also, the cleaning liquid in the wells O5 and O6 moves to the wells O6 and O9.

In the well O10, DNA is trapped in the DNA trapping agent, and the magnetic particles that are the trapping agent are trapped in the well O10 by applying a magnetic field.
Further, when each roller rotates and moves in the direction of the solid arrow for one well, the waste liquid after the DNA trap in the well O10 moves to the well O11. Further, the cleaning liquid in the well O9 moves to the well O10, and the cleaning liquid in the well O6 moves to the well O9. In the well O10, the first cleaning with the magnetic particle cleaning liquid is performed.

  Further, when each roller rotates and moves in the direction of the arrow for one well, the waste liquid in the well O11 moves to the well O12. The cleaning liquid after cleaning the well O10 moves to the well O11. The second cleaning liquid is sent to the well O10, removed from the well O10 by the next movement of the roller, and the first cleaning liquid 167a is sent to the well O12, and the second cleaning liquid 167b is sent to the well O11.

As a result, DNA trapped magnetic particles 166 remain in the well O10, and DNA can be extracted.
In parallel with the above-described operation, each roller in the lower stage P2 proceeds in the direction of the solid line arrow in synchronization with the roller in the upper stage P1, and the extraction buffer solution 165 proceeds to the well O19 as indicated by the broken line arrow. The wells O16 to O18 are originally empty wells, and are dummy wells for adjusting the liquid feeding timing of the extraction buffer solution 165 to the well O10. Since there is this dummy well, the liquid feeding timing can be arbitrarily adjusted only by the movement of the roller in one axial direction.

At this point, the well O10 has been cleaned twice, and the cleaning liquid has been removed from the well O10.
Next, the upper P1 roller group is locked, and only the lower roller group moves,
The buffer solution for extraction in the well O19 in the lower stage P2 is sent to the well O10 as indicated by the broken line arrow, and the DNA is released in the well O10 (FIG. 19B).

Here, when the upper and lower roller groups P1 and P2 move simultaneously, the DNA extract 168 (result) in the well O10 is sent to the well O20, and the DNA extraction operation is completed (FIG. 19C). ).
The mechanism of liquid feeding described here is similar to the operation of a shift register of a digital circuit. Therefore, it can be said that the movement of the roller group is a clock type.

FIG. 20 is an explanatory diagram showing a fourteenth embodiment relating to the cartridge and the drive mechanism. In this embodiment, the arrangement of the rollers indicated by the oblique lines in the thirteenth embodiment (FIG. 19) is changed from a two-stage configuration to a three-stage configuration, and a roller group is added at the same pitch to the middle stage P3, and the positional relationship in the horizontal direction is As it is, the vertical position of the well O10 was arranged in the middle stage P3. The plurality of rollers in the upper stage P1 and the lower stage P2 are arranged at intervals corresponding to the intervals in the well lateral direction and seal the flow paths connecting the wells.
In FIG. 20, the well O1 is a sample solution, the well O2 is a solution, the well O3 is a DNA trapping agent (surface-modified magnetic particles), the wells O4 and O5 are cleaning solutions, and the well O15 is an extraction buffer. Pre-filled with liquid. The other wells are in a zero volume state.

In this embodiment, the well structure is a zero volume structure, but it may be a structure provided with an exhaust passage. Further, although a roller is used as the pressurizing means, a piston type actuator may be used.
By moving the rollers of each stage for 5 wells in the direction of the solid line arrow, the mixed solution of the sample solution and the solution and the DNA trapping agent are sent to the well O10 and the DNA is trapped by the magnetic particles, and the washing solutions 167a and 167b are The washed cleaning liquids 167a and 167b are sent to the wells O11 and O12. As a result, the DNA particles having been trapped with DNA 166 are present in the well O10.

At this time, since the roller in the lower stage P2 is also moving in the same manner, the extraction buffer liquid 165 in the well O15 is also moved to the well O19 as indicated by the broken line arrow.
Next, while the upper P1 roller group is stopped, the middle P3 and lower P2 roller groups move one well in the direction of the solid arrow, and the extraction buffer solution 165 in the well O19 is sent to the well O10, and the DNA Is released from the trapping agent. Then, the roller group of the middle stage P3 and the lower stage P2 moves again by one well, and the DNA extract (product) is sent from the well O10 to the well O20.

In this way, in the DNA extraction process, the upper P1 roller seals the flow path connecting the well O10 and the wells O7, O8, O9, O11. ) Can be further prevented.

FIG. 21 is an explanatory view showing a fifteenth embodiment relating to the cartridge and the drive mechanism.
In FIG. 21A, wells indicated by vertical lines are arranged side by side in the vertical and horizontal directions, and wells adjacent in the vertical and horizontal directions are connected by a flow path. In such an arrangement, the hatched rollers are independently arranged so as to sandwich the well for each row or column of the well. By sequentially moving the vertical (X-axis) and horizontal (Y-axis) roller groups, the liquid can be moved to a well at an arbitrary position.

To prevent the X and Y rollers from interfering with each other, when the X and Y roller groups pressurize and feed liquid from the same surface, when X moves, Y is separated from the cartridge. Or what is necessary is just to arrange | position X and Y on the back surface and the surface, respectively. As the cartridge in this case, a cartridge having a structure in which a substrate as shown in FIG.
The configuration of the roller group is such that the entire row or column moves together, or several of the rows or columns move together.
The flow path is not limited to a vertical and horizontal mesh shape as shown in FIG. 21, and there may be a region without a flow path between wells, or may be provided obliquely as shown in FIG. Further, the size and depth of the well may be different from each other.

In FIG. 21B, by setting the flow paths obliquely, the liquid can be moved in any direction of XY even with one direction roller.
For example, as shown in FIG. 21B, when the roller group is made up of three stages and the roller group of the upper stage R1, the roller group of the middle stage R2, and the roller group of the lower stage R3 are moved synchronously, the liquid in the well Q1 is When moving to the wells Q2, Q3 and moving the roller group of the upper stage R1 and the roller group of the middle stage in synchronization, the solution in the well Q3 moves to the wells Q4, Q5, Q6. Furthermore, when the roller group of the middle stage R2 and the roller group of the lower stage R3 are moved, the liquid moves to the well Q7.

  In such a structure in which the rollers are provided on the XY axes, liquid tends to remain in the flow path between the rollers. In this case, as shown in FIG. 21C, the entire flow path can be sealed by providing a rigid body 170 in the flow path portion between the elastic body 171 and the substrate 172 and applying pressure by the roller 169. . The rigid body 170 can be formed, for example, by being embedded in the elastic body 171 or by curing a part of the elastic body 171.

FIG. 22 is an explanatory view showing a sixteenth embodiment relating to the cartridge and the drive mechanism.
Although the roller or piston type actuator has been exemplified as the pressurizing means in the embodiments so far, as shown in FIG. 22, the pressurizing means 173 is a two-dimensional shape having a curvature on the contact surface with the container such as the well 175. A plate or a caterpillar (registered trademark) may be used to press against the cartridge 174 and move in the arrow direction. According to this, since the flow path and the well are pushed by the surface, the return of the solution and air due to the back pressure can be prevented.

  The present invention is not limited to the above-described embodiments, and includes many changes and modifications without departing from the essence thereof.

It is an external view which shows one Example of the cartridge for chemical reaction which concerns on this invention. It is explanatory drawing explaining the liquid feeding and exhaust_gas | exhaustion which concern on this invention. It is explanatory drawing which shows the other Example (zero volume structure) of the cartridge for chemical reactions which concerns on this invention. It is explanatory drawing explaining the 1st Example of the manufacturing method of a cartridge. It is explanatory drawing explaining the 2nd Example of the manufacturing method of a cartridge. It is explanatory drawing explaining the 3rd Example of the manufacturing method of a cartridge. It is explanatory drawing which shows the 1st Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 2nd Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 3rd Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 4th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 5th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 6th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 7th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 8th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 9th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 10th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 11th Example concerning a cartridge and a drive mechanism. It is explanatory drawing which shows the 12th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 13th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 14th Example which concerns on a cartridge and a drive mechanism. It is explanatory drawing which shows the 15th Example concerning a cartridge and a drive mechanism. It is explanatory drawing which shows the 16th Example which concerns on a cartridge and a drive mechanism. It is a block diagram of the conventional biochip. It is explanatory drawing explaining the operation method of the conventional biochip.

Explanation of symbols

DESCRIPTION OF SYMBOLS 101 Cartridge 102 Elastic body 103 Board | substrate 104 Common intake path 104a-104c Intake path 105a-105f Flow path 106 Common exhaust path 106a-106c Exhaust path A1-A7 Well 107a, 107b Channel 108 Common exhaust path 108a, 108b Exhaust path 109 Roller 110 Air B1, B2 Well 111 Cartridge 112-115 Flow path 116 Roller 117 Elastic body 118 Substrate C1-C3 Well 119 Mask 120 Substrate 121 Non-adhesive part 122 Substrate 123 Elastic body 124 Adhesive 125 Non-adhesive part 126 Substrate 127 Elastic body 128 Embedding agent 129 Non-adhesive part 130a, 130b Roller 131a, 131b Flow path D1, D2 Well 132a-132f Roller 133a-133e Flow path E1-E6 Well 134a, 1 4b roller F1 to F13 well 135 cartridge 136 syringe 137 introduction port 138 flow path 139a, 139b roller 140a, 140b injection portion 141 introduction port 142 solution 143a roller 144a, 144b flow path G1, G2 well 145a to 145d well shutter H14 to H5 H5 shutter H14 146b Roller I1-I5 Well 147a, 147b Roller J1-J5 Well 148a-148c Roller K1-K5 Well 149a-149m Roller L1-L14 Well 150 Cartridge 151a, 151b Elastic body 152 Substrate 153 Through hole 154 Front surface 155 155 Flow path 157a to 157c Roller M1 to M3 Well 158 Sample 159 Trapping agent 160 DNA buffer solution 161a to 61c rollers 162 sample 163 trapping agent 164 DNA buffer solution N1~N3 well 165 extraction buffer solution 166 DNA trap already magnetic particles 167a, 167b cleaning liquid 168 DNA extract O1~O23 well Q1~Q7 well 169 roller 170 rigid 171 elastic body
172 Substrate 173 Pressurizing means 174 Cartridge 175 Well 176a to 176f Convex part

Claims (50)

It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A chemical reaction cartridge comprising an exhaust passage for exhausting indoor air into a chamber into which at least the fluid substance is introduced. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A chemical reaction cartridge, wherein at least one of the flow path and the chamber has a volume of zero before the fluid substance is introduced. A method for producing a cartridge according to claim 2,
A method for manufacturing a cartridge for chemical reaction, wherein a non-adhesive portion that does not adhere the flow path or the chamber portion is formed when the rigid body and the elastic body are bonded.   4. The method for producing a chemical reaction cartridge according to claim 3, wherein the non-adhesive portion is formed by applying a non-adhesive substance before bonding.   4. The method for producing a cartridge for chemical reaction according to claim 3, wherein the non-adhesive portion is formed by applying a mask or a substance that is not activated by plasma in a corresponding region during plasma processing.   4. The chemical reaction cartridge according to claim 3, wherein the non-adhesive portion is formed by applying an adhesive around a corresponding region and not applying the adhesive to the non-adhesive portion. 5. Method.   4. The chemical reaction cartridge according to claim 3, wherein the non-adhering portion forms the non-adhesive surface by embedding an embedding material having at least one non-adhesive surface in the substrate. Manufacturing method.   The non-adhesive portion is formed by placing an embedding material having a non-adhesive surface on at least one surface on the previously cured substrate or the elastic body, and then molding the corresponding elastic body or substrate by casting. The method for producing a cartridge for chemical reaction according to claim 3. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A drive mechanism for a chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
A drive mechanism for a chemical reaction cartridge having pressurizing means for simultaneously sealing all input and output channels connected to the chamber holding the fluid substance. A drive mechanism for a chemical reaction cartridge according to claim 1 or 2,
A drive mechanism for a chemical reaction cartridge, comprising pressurizing means for simultaneously sealing all the flow paths connected to the chamber holding the fluid substance.   11. The driving mechanism for a chemical reaction cartridge according to claim 9, wherein the pressurizing means is a roller used for moving a fluid substance.   11. The driving mechanism for a chemical reaction cartridge according to claim 9, wherein at least one of the pressurizing means is a shutter.   11. The chemical reaction cartridge driving mechanism according to claim 9, wherein the sealing of the fluid substance is simultaneously performed at a plurality of locations in one cartridge.   11. The chemical reaction cartridge drive mechanism according to claim 9, wherein the sealing of the fluid substance is performed at each reaction step. 11. The drive mechanism for a chemical reaction cartridge according to claim 9, wherein the pressure in the chamber is changed by moving at least one of the pressurizing means after sealing the fluid substance. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber provided with a plurality of inflow channels into which a plurality of different fluid substances flows and at least one discharge channel.
At least one of the chambers is a dummy chamber for adjusting a timing for feeding a predetermined fluid substance to the common chamber. The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber provided with a plurality of inflow channels into which a plurality of different fluid substances flows and at least one discharge channel.
3. The chemical reaction according to claim 1, wherein at least one of the chambers is a dummy chamber for adjusting a timing at which a predetermined fluid substance is fed to the common chamber. Cartridge. A drive mechanism for a chemical reaction cartridge according to claim 16 or 17,
A plurality of pressurizing means provided at positions for sealing the flow paths of the plurality of chambers;
A drive mechanism for a chemical reaction cartridge, wherein the plurality of pressurizing means simultaneously move in the same direction and feed liquid one pitch at a time. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The inlet for the fluid substance is composed of a plurality of injection parts coupled inside,
A cartridge for chemical reaction, characterized in that internal air is discharged from another injection part by injection of a fluid substance from one injection part. The inlet for the fluid substance is composed of a plurality of injection parts coupled inside,
The cartridge for chemical reaction according to claim 1 or 2, wherein the internal air is discharged from the other injection part by injection of the fluid substance from one injection part. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
An inlet for storing a predetermined amount of the fluid substance;
Means for sucking a certain amount of fluid substance at the inlet into the interior,
A cartridge for chemical reaction, comprising: An inlet for storing a predetermined amount of the fluid substance;
Means for sucking a certain amount of fluid substance at the inlet into the interior,
The cartridge for chemical reaction according to claim 1, wherein the cartridge for chemical reaction is provided. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber provided with an inflow channel into which two or more different types of fluid substances flow, and at least two discharge channels,
When one fluidic substance flows into and out of the common chamber, the flow of the other fluidic substance and at least one outlet flow path are sealed by the external force. cartridge. At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
2. When one fluidic substance flows into and out of the common chamber, the flow of the other fluidic substance and at least one flow-out channel are sealed by the external force. Or the cartridge for chemical reaction of Claim 2.   25. The flow path through which the other fluid substance flows in and out is formed at a position sealed by an external force for feeding the one fluid substance. The cartridge for chemical reaction as described.   The plurality of chambers have a convex portion that is pressed down when the external force is applied, outside the container, and the flow path for feeding the one fluid substance is formed in a concave portion between the convex portions. The cartridge for chemical reaction according to claim 23 or 24. A drive mechanism for a chemical reaction cartridge according to claim 23 or claim 24,
The flow path through which the other fluid substances flow in and out is simultaneously sealed by a wheel-type pressurizing means, and the chemical reaction cartridge drive mechanism.   The wheel-type pressurizing unit seals a flow path through which the other fluid-like substance flows in and out by movement or rotational movement in the same axial direction as the pressurizing unit that performs liquid feeding. Item 28. The chemical reaction cartridge drive mechanism according to Item 27. A drive mechanism for a chemical reaction cartridge according to claim 23 or claim 24,
The inflow passage and the outflow passage of the common chamber are formed radially around the common chamber, and the pressurizing means for feeding the respective passages is provided within the surface of the container. The mechanism for driving a chemical reaction cartridge according to claim 1, wherein the mechanism moves in different axial directions in accordance with the respective flow paths. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
The chemical reaction cartridge, wherein the plurality of flow paths passing through the common chamber are respectively disposed on a back surface and a front surface of the cartridge. At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
3. The chemical reaction cartridge according to claim 1, wherein the plurality of flow paths passing through the common chamber are respectively disposed on a back surface and a front surface of the cartridge. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
At least one of the chambers is a common chamber having flow paths through which two or more different fluid substances flow in and out, respectively.
The flow path of this common chamber is arranged adjacent to the common chamber in a straight line, and when one fluidic substance flows into and out of the common chamber, the other fluidic substance The cartridge for chemical reaction, wherein the inflow channel is sealed by the external force. At least one of the chambers is a common chamber having flow paths through which two or more different fluid substances flow in and out, respectively.
The flow path of this common chamber is arranged adjacent to the common chamber in a straight line, and when one fluidic substance flows into and out of the common chamber, the other fluidic substance The cartridge for chemical reaction according to claim 1 or 2, wherein the inflow channel is sealed by the external force. A drive mechanism for a chemical reaction cartridge according to claim 32 or claim 33,
The pressurizing means for feeding liquid to the common chamber is installed so as to sandwich the chamber with the fluid substance to be fed, and moves in the straight direction in the container surface. Drive mechanism for chemical reaction cartridge. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber provided with an inflow channel into which two or more different types of fluid substances flow, and at least two discharge channels,
A chemical reaction cartridge, wherein when one fluid substance flows into and out of the common chamber, a flow path into which another fluid substance flows is sealed by the external force. The plurality of chambers are arranged at equal intervals,
At least one of the chambers is a common chamber including an inflow channel into which two or more different fluid substances flow respectively and at least two discharge channels.
3. When one fluid substance flows into and out of the common chamber, a flow path into which another fluid substance flows is sealed by the external force. The cartridge for chemical reaction as described. A drive mechanism for a chemical reaction cartridge according to claim 35 or claim 36,
A mechanism for driving a chemical reaction cartridge, wherein the pressurizing means for feeding different fluid substances are a plurality of pressurizing means groups that move independently from each other.   38. The chemical reaction cartridge drive mechanism according to claim 37, wherein the plurality of pressurizing means groups move in at least two different directions within the container surface.   39. The mechanism for driving a chemical reaction cartridge according to claim 38, wherein the two directions are directions different by 90 degrees.   37. The chemical reaction cartridge according to claim 35 or 36, wherein a moving direction of the pressurizing means for applying the external force and a direction of the flow path are different in the container surface.   41. The chemical reaction cartridge according to claim 40, wherein the direction of the flow path forms an angle of 90 degrees or less with respect to the moving direction of the pressurizing means.   37. A rigid body is formed on the container side of the flow path, and the entire flow path of the rigid body forming portion is sealed by applying an external force to a part of the flow path. 40. The cartridge for chemical reaction according to claim 41.   The trapping agent for trapping a predetermined substance is permanently or temporarily fixed in the common chamber, wherein the common chamber is fixed to the common chamber. 33, 35, 36, 40, 41 or a cartridge for chemical reaction according to claim 42.   44. The chemical reaction cartridge according to claim 43, wherein the predetermined substance is a biopolymer. 45. The chemical reaction cartridge according to claim 44, wherein the biopolymer is DNA, RNA, protein, or sugar chain. 44. The chemical reaction cartridge according to claim 43, wherein the trapping agent is a bead, a filter, or a column whose surface is modified for trapping.   The cartridge for chemical reaction according to claim 46, wherein the beads are silica, magnetic beads, metal beads, or resin beads. It is composed of a container at least partly formed of an elastic body,
In the container, a plurality of chambers arranged to be connected or connectable with a flow path are formed,
A chemical reaction cartridge for performing a chemical reaction by moving a fluid substance in the flow path or the chamber or both by applying an external force to the elastic body from outside the container,
2. The chemical reaction cartridge driving mechanism according to claim 1, wherein the external force is generated by pressing a two-dimensional plate from one direction on the elastic body side. A drive mechanism for a chemical reaction cartridge according to claim 1 or 2,
2. The chemical reaction cartridge driving mechanism according to claim 1, wherein the external force is generated by pressing a two-dimensional plate from one direction on the elastic body side. 50. The drive mechanism for a chemical reaction cartridge according to claim 48, wherein the plate has a curvature on a surface to which the external force is applied to the flow path or the chamber.

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