JP2005290107A5 - - Google Patents
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- Publication number
- JP2005290107A5 JP2005290107A5 JP2004104557A JP2004104557A JP2005290107A5 JP 2005290107 A5 JP2005290107 A5 JP 2005290107A5 JP 2004104557 A JP2004104557 A JP 2004104557A JP 2004104557 A JP2004104557 A JP 2004104557A JP 2005290107 A5 JP2005290107 A5 JP 2005290107A5
- Authority
- JP
- Japan
- Prior art keywords
- isopropylacrylamide
- converging
- linking agent
- cross
- hollow part
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003431 cross linking reagent Substances 0.000 claims 2
- QNILTEGFHQSKFF-UHFFFAOYSA-N n-propan-2-ylprop-2-enamide Chemical compound CC(C)NC(=O)C=C QNILTEGFHQSKFF-UHFFFAOYSA-N 0.000 claims 2
- 238000007334 copolymerization reaction Methods 0.000 claims 1
- 238000005520 cutting process Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 238000002493 microarray Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
Claims (1)
（１） 複数本の管状体をそれらの長手方向が一致するように集束する工程。
（２） 集束物の各管状体の中空部に、N-イソプロピルアクリルアミド、架橋剤及び
キャプチャープローブを含む溶液であって、N-イソプロピルアクリルアミドと架橋剤のモル比が、10/1〜500/1である溶液を充填し、中空部内で共重合反応する工程。
（３） 集束物の長手方向と交叉する方向で切断する工程。 A manufacturing method of a microarray including the following steps in sequence.
(1) A step of converging a plurality of tubular bodies so that their longitudinal directions coincide.
(2) A solution containing N-isopropylacrylamide, a cross-linking agent and a capture probe in the hollow part of each tubular body of the converging object, wherein the molar ratio of N-isopropylacrylamide and cross-linking agent is 10/1 to 500/1. The process which is filled with the solution which is and carries out a copolymerization reaction in a hollow part.
(3) A step of cutting in a direction crossing the longitudinal direction of the convergent object.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004104557A JP2005290107A (en) | 2004-03-31 | 2004-03-31 | Gel with fixed capture probe and capture probe-immobilized gel microarray using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004104557A JP2005290107A (en) | 2004-03-31 | 2004-03-31 | Gel with fixed capture probe and capture probe-immobilized gel microarray using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005290107A JP2005290107A (en) | 2005-10-20 |
| JP2005290107A5 true JP2005290107A5 (en) | 2007-05-24 |
Family
ID=35323416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004104557A Pending JP2005290107A (en) | 2004-03-31 | 2004-03-31 | Gel with fixed capture probe and capture probe-immobilized gel microarray using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2005290107A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009145058A (en) * | 2007-12-11 | 2009-07-02 | Mitsubishi Rayon Co Ltd | Nucleic acid microarray and manufacturing method thereof |
| SG192251A1 (en) * | 2011-02-10 | 2013-09-30 | Mitsubishi Rayon Co | Method for detecting nucleic acid |
| JP5660468B2 (en) * | 2011-03-15 | 2015-01-28 | 三菱レイヨン株式会社 | Method for producing gel microarray for detecting bio-related substances |
| JP5910892B2 (en) * | 2014-02-10 | 2016-04-27 | 三菱レイヨン株式会社 | Nucleic acid microarray and manufacturing method thereof |
-
2004
- 2004-03-31 JP JP2004104557A patent/JP2005290107A/en active Pending
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