JP2005287964A5 - - Google Patents

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Publication number
JP2005287964A5
JP2005287964A5 JP2004110427A JP2004110427A JP2005287964A5 JP 2005287964 A5 JP2005287964 A5 JP 2005287964A5 JP 2004110427 A JP2004110427 A JP 2004110427A JP 2004110427 A JP2004110427 A JP 2004110427A JP 2005287964 A5 JP2005287964 A5 JP 2005287964A5
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JP
Japan
Prior art keywords
tissue
organ
living body
image
fluorescent
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Pending
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JP2004110427A
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Japanese (ja)
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JP2005287964A (en
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Priority to JP2004110427A priority Critical patent/JP2005287964A/en
Priority claimed from JP2004110427A external-priority patent/JP2005287964A/en
Priority to US10/594,311 priority patent/US7589839B2/en
Priority to PCT/JP2005/005898 priority patent/WO2005096059A1/en
Priority to EP05727465.6A priority patent/EP1731941B1/en
Publication of JP2005287964A publication Critical patent/JP2005287964A/en
Publication of JP2005287964A5 publication Critical patent/JP2005287964A5/ja
Pending legal-status Critical Current

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Claims (10)

生体・臓器・組織を内から照明し、生体・臓器・組織を外から撮影する、生体・臓器・組織の観察方法。   A method for observing a living body / organ / tissue by illuminating the living body / organ / tissue from the inside and photographing the living body / organ / tissue from the outside. 請求項1において、照明光・励起光を外部に射出する光射出部を生体・臓器・組織に導入し、光射出部から照明光・励起光を射出して生体・臓器・組織を内から照明し、生体・臓器・組織を外から撮影して生体・臓器・組織の透過像と蛍光像の少なくとも一方の光学像を取得し、取得した光学像の画像を表示装置に表示する、生体・臓器・組織の観察方法。   The light emitting unit for emitting illumination light / excitation light to the outside is introduced into the living body / organ / tissue, and the living body / organ / tissue is illuminated from inside by emitting the illumination light / excitation light from the light emitting unit. The living body / organ / tissue is photographed from outside to acquire an optical image of at least one of the transmission image and the fluorescent image of the living body / organ / tissue, and the acquired optical image is displayed on the display device.・ Tissue observation method. 請求項2において、生体・臓器・組織の腔に光射出部を挿入して照明する、生体・臓器・組織の観察方法。   The method for observing a living body / organ / tissue according to claim 2, wherein the light emitting part is inserted into the cavity of the living body / organ / tissue and illuminated. 請求項2において、光射出部を生体・臓器・組織に刺して照明する、生体・臓器・組織の観察方法。   The method for observing a living body / organ / tissue according to claim 2, wherein the light emitting part is stabbed into a living body / organ / tissue for illumination. 請求項2において、光射出部を生体・臓器・組織に押し当てて照明する、生体・臓器・組織の観察方法。   The method for observing a living body / organ / tissue according to claim 2, wherein the light emitting unit is pressed against the living body / organ / tissue for illumination. 照明光・励起光を外部に射出する光射出部を生体・臓器・組織に導入し、光射出部から照明光・励起光を射出して生体・臓器・組織を内から照明し、生体・臓器・組織を外から撮影して生体・臓器・組織の蛍光像を取得し、取得した蛍光像の画像を他の画像と比較して生体・臓器・組織内の蛍光物質の量や面積の経時的変化を比較したり検討したりする、生体・臓器・組織を用いた実験方法。   A light emitting part that emits illumination light / excitation light to the outside is introduced into the living body / organ / tissue, and illumination light / excitation light is emitted from the light emitting part to illuminate the living body / organ / tissue from the inside.・ Tissue is photographed from outside to obtain fluorescent images of living body / organ / tissue, and the amount of fluorescent substance in the living body / organ / tissue is compared with other images over time. Experimental methods using living organisms, organs, and tissues that compare and examine changes. 請求項6において、さらに生体・臓器・組織を内から撮影して生体・臓器・組織の蛍光像を取得し、生体・臓器・組織を外から撮影した蛍光像の画像と生体・臓器・組織を内から撮影した蛍光像の画像とを用いて生体・臓器・組織内の蛍光物質の量や面積の変化をミクロ的かつマクロ的に観察することによって蛍光物質の量や面積の経時変化を比較または検討する、生体・臓器・組織を用いた実験方法。   7. The fluorescent image of the living body / organ / tissue obtained from the inside by acquiring a fluorescent image of the living body / organ / tissue from outside, Compare changes in the amount and area of fluorescent substances over time by observing changes in the quantity and area of fluorescent substances in living organisms, organs and tissues using microscopic and macro images taken from inside. Experimental methods using living organisms, organs, and tissues to be examined. 請求項6において、生体・臓器・組織を外から撮影して透過像と蛍光像とを取得し、透過像の画像と蛍光像の画像とに基づいて生体・臓器・組織内の蛍光物質の位置や量や面積を検討する、生体・臓器・組織を用いた実験方法。   7. The position of the fluorescent substance in the living body / organ / tissue according to claim 6, wherein a living body / organ / tissue is photographed from outside to obtain a transmission image and a fluorescence image, and the transmission image and the fluorescence image are obtained based on the transmission image and the fluorescence image. Experimental methods using living organisms, organs, and tissues to examine the amount and area. 請求項6において、生体・臓器・組織を外から撮影して蛍光像を取得し、蛍光像の画像と蛍光像の微分画像とに基づいて生体・臓器・組織内の蛍光物質の位置や量や面積を検討する、生体・臓器・組織を用いた実験方法。   In Claim 6, the living body / organ / tissue is photographed from outside to obtain a fluorescent image, and the position and amount of the fluorescent substance in the living body / organ / tissue are determined based on the fluorescent image and the differential image of the fluorescent image. An experimental method using living organisms, organs, and tissues to examine the area. 請求項6において、波長が異なる複数種類の励起光で生体・臓器・組織を内から照明し、生体・臓器・組織を外から撮影して取得した複数の蛍光像の画像に基づいて生体・臓器・組織の自家蛍光を分離して生体・臓器・組織の少なくとも一つの蛍光像の画像を得ることによって生体・臓器・組織内の蛍光物質の位置や量や面積を検討する、生体・臓器・組織を用いた実験方法。   7. The living body / organ as claimed in claim 6, wherein the living body / organ / tissue is illuminated from the inside with a plurality of types of excitation light having different wavelengths, and the living body / organ / tissue is photographed from outside to obtain images of the plurality of fluorescent images.・ Determine the position, amount, and area of fluorescent substances in a living body, organ, or tissue by separating the autofluorescence of the tissue and obtaining an image of at least one fluorescent image of the living body, organ, or tissue. Experimental method using
JP2004110427A 2004-03-31 2004-04-02 Observation apparatus for observing living body, organ and tissue Pending JP2005287964A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004110427A JP2005287964A (en) 2004-04-02 2004-04-02 Observation apparatus for observing living body, organ and tissue
US10/594,311 US7589839B2 (en) 2004-03-31 2005-03-29 Examination apparatus, fluoroscopy apparatus, examination method, and experimental method
PCT/JP2005/005898 WO2005096059A1 (en) 2004-03-31 2005-03-29 Observing device and fluorescent light observing device
EP05727465.6A EP1731941B1 (en) 2004-03-31 2005-03-29 Observing device and fluorescent light observing device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004110427A JP2005287964A (en) 2004-04-02 2004-04-02 Observation apparatus for observing living body, organ and tissue

Publications (2)

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JP2005287964A JP2005287964A (en) 2005-10-20
JP2005287964A5 true JP2005287964A5 (en) 2007-05-24

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JP2004110427A Pending JP2005287964A (en) 2004-03-31 2004-04-02 Observation apparatus for observing living body, organ and tissue

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WO2008123610A1 (en) * 2007-04-04 2008-10-16 Olympus Corporation Long-time monitoring method using photoprotein and analysis method
US8031924B2 (en) * 2007-11-30 2011-10-04 General Electric Company Methods and systems for removing autofluorescence from images
JP2010068860A (en) * 2008-09-16 2010-04-02 Fujifilm Corp Endoscope apparatus and image processing method for the same
JP5360540B2 (en) * 2008-10-02 2013-12-04 公立大学法人広島市立大学 Brain function measurement device
WO2010061471A1 (en) * 2008-11-28 2010-06-03 オリンパス株式会社 Living body observation apparatus
US20120029348A1 (en) * 2009-04-14 2012-02-02 The Gemeral Hospital Corporation Method and apparatus for multimodal imaging of biological tissue
US9002439B2 (en) 2009-05-13 2015-04-07 Sumitomo Electric Industries, Ltd. Blood vessel wall analyzing device and blood vessel wall analyzing method
JP5435796B2 (en) * 2010-02-18 2014-03-05 富士フイルム株式会社 Method of operating image acquisition apparatus and image pickup apparatus
WO2011150379A2 (en) * 2010-05-28 2011-12-01 The General Hospital Corporation Apparatus, systems, methods and computer-accessible medium for analyzing information regarding cardiovascular disease(s) and function(s)
EP2579084B1 (en) * 2010-06-03 2023-11-08 Nikon Corporation Microscope device
JP5713387B2 (en) * 2011-01-28 2015-05-07 学校法人慈恵大学 Trace optical imaging apparatus and trace optical imaging method
JP6404687B2 (en) * 2014-11-21 2018-10-10 倉敷紡績株式会社 Fluorescence imaging apparatus and fluorescence imaging method
CN109561870A (en) * 2016-08-08 2019-04-02 国立大学法人京都大学 Excision process estimation device and excision process wizard system

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US5555885A (en) * 1988-12-21 1996-09-17 Non-Invasive Technology, Inc. Examination of breast tissue using time-resolved spectroscopy
JPH0759782A (en) * 1993-08-23 1995-03-07 Hitachi Ltd Living body light measuring apparatus
JP3117178B2 (en) * 1994-09-26 2000-12-11 友佑 野々村 Oral tissue observation device
JPH09294705A (en) * 1996-04-30 1997-11-18 Fuji Photo Film Co Ltd Fluorescent endoscope

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