JP2005278454A - Method for collecting microorganism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for collecting microorganisms by which the collected microorganisms can be examined in good accuracy in a filter for collecting the microorganisms which is used for an apparatus for rapidly examining the microorganisms. <P>SOLUTION: The method for collecting the microorganisms can improve the accuracy for measuring the microorganisms by limiting the range for collecting the microorganisms of the target to be collected by limiting the range of filtration of a liquid specimen by installing a filtration range-limiting means 1 of the liquid specimen containing the microorganisms on the microorganism-capturing face of the filter 103 for collecting the microorganisms. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for collecting microorganisms using a filter for collecting microorganisms used for examining microorganisms.

Conventionally, this kind of microorganism collection method is known from Patent Document 1.
The microorganism collection method will be described below with reference to FIG.

  As shown in FIG. 5, the filtration member 101 that collects a liquid specimen is composed of a filter holder 102, a filter 103, and a filter receiver 104.

In the above configuration, a liquid sample containing microorganisms was dropped onto the center of the filtration plate, and the dropped sample was sucked from the back of the filter using a suction device to collect the microorganisms.
JP 2003-114175 A

  In such a conventional method for collecting microorganisms, when a liquid sample containing microorganisms is filtered, the liquid sample is filtered in a state where the liquid sample surrounds the filter holder, and the microorganisms are collected over the entire area of the filter. Become.

  Furthermore, in the microorganism rapid measurement apparatus that measures using a filtration member in which microorganisms are collected, the microorganisms collected on the filter surface are fluorescently stained, and the microorganisms on the filter emit fluorescent light to display an image of the light. A method of taking a picture and measuring a luminescence point of a microorganism by image processing is taken. Since this measurement method is performed by taking an image, it is necessary to be in focus, and in order to achieve focus, it is necessary to obtain the flatness of the filter surface. In order to obtain this flatness, a method is adopted in which microorganisms are photographed by pressing a measurement table smaller than the filtration part from the back of the filter. For this reason, the flatness of the outer peripheral part of the filtration part removed from the measurement table does not come out, and since there is no focus at the time of photographing the luminescent spot, there is a problem that it is impossible to measure all the collected microorganisms It is required that all the collected microorganisms can be measured by limiting the collection range.

  The present invention solves such a conventional problem, and aims to provide a microorganism collection method that limits the filtration range of a liquid specimen containing microorganisms to be injected and can limit the microorganism collection range. .

  The microorganism collection method of the present invention is provided with a filtration range limiting means for limiting the filtration range in order to achieve the above object, so that the microorganism collection range of the filter for collecting microorganisms can be limited. .

  By this means, the filtration range of the liquid specimen can be limited, and a microorganism collection method that can limit the collection range of microorganisms can be provided.

  Another means is a structure in which the filtering range limiting means of the filter is provided with an opening.

  Thereby, the filtration range of a liquid sample can be limited, and the microorganisms collection method which can limit the collection range of microorganisms can be provided.

  Another means is a tapered structure in which a taper is provided inside the structure so that the microorganism collection side is smaller than the liquid sample injection side.

  Thereby, the filtration range of the liquid specimen can be limited, and a microorganism collection method capable of limiting the collection range of microorganisms with improved filtration performance can be provided.

    In another aspect, the structure and the tapered structure are variable height structures capable of varying the height.

  Thereby, the filtration range of a liquid sample can be limited, and the microorganism collection method which can limit the collection range of the microorganisms with a good liquid sample injection property can be provided.

  Another means is a water repellent treatment structure obtained by subjecting the structure, the tapered structure, and the variable height structure to water filtration.

  Thereby, the filtration range of a liquid specimen can be limited, and a microorganism collection method capable of limiting the collection range of microorganisms with good liquid specimen injectability and filterability can be provided.

  The other means is configured such that the outer periphery of the microorganism collection surface of the microorganism collection filter is subjected to non-filtration treatment.

  Thereby, the filtration range of a liquid sample can be limited, and the microorganisms collection method which can limit the collection range of microorganisms can be provided.

  Further, the other means is configured to perform the non-filtration treatment of the filter with an adhesive.

  Thereby, the filtration range of a liquid sample can be limited, and the microorganisms collection method which can limit the collection range of microorganisms can be provided.

  Further, the other means is configured such that the non-filtration treatment of the filter is performed with a colored paint.

  Thereby, the filtration range of a liquid specimen can be limited, and the microorganism collection method which can limit the collection range of a microorganism with good visibility of the injection position of a liquid specimen can be provided.

  In another method, the non-filtration treatment of the filter is performed by a water repellent treatment.

  Thereby, the filtration range of a liquid specimen can be limited by this, and the microorganisms collection method which can limit the collection range of microorganisms with good filtration performance can be provided.

  ADVANTAGE OF THE INVENTION According to this invention, the microorganisms collection method which has the effect that the measurement precision of microorganisms can be improved can be provided because the microorganisms collection range can be limited.

  In addition, according to the present invention, it is possible to provide a microorganism collection method having an effect of shortening the liquid sample injection time by improving the sample injection property.

  Moreover, according to this invention, the microorganisms collection method which has the effect that filtration time can be shortened by improving filtration performance can be provided.

  The invention according to claim 1 of the present invention is provided with a means for limiting the filtration range of the liquid specimen containing microorganisms injected into the filter for collecting microorganisms, and can act to limit the range in which microorganisms are collected. Have

  The invention according to claim 2 of the present invention is that the filtration range limiting means is a cylindrical structure provided with an opening, and has the effect that the liquid sample injection position can be limited and the microorganism collection range can be limited. .

  According to the third aspect of the present invention, the filtering range limiting means is a tapered structure body having a taper that is smaller in the diameter of the microorganism collection side than the diameter of the opening and the liquid sample injection side. The trapping range can be limited and the filterability can be improved.

  The invention according to claim 4 of the present invention is such that the filtration range limiting means is a variable height structure capable of changing the height of the cylindrical structure and the tapered structure, and the microbial collection range is limited. And has the effect of improving the liquid specimen injection property.

  In the invention according to claim 5 of the present invention, the filtration range limiting means is obtained by subjecting the cylindrical structure, the tapered structure, and the variable height structure to a water repellent treatment, so that the microbial collection range can be limited. It has the effect of improving the filterability and improving the liquid sample injection property.

  The invention according to claim 6 of the present invention is configured such that the filtration limiting means is subjected to an unfiltering process so that the outer periphery of the filter for collecting microorganisms cannot be filtered, and has an effect of limiting the range of collecting microorganisms. Have.

  Invention of Claim 7 of this invention is set as the structure which performs an unfiltering process with an adhesive agent, and has the effect | action which can limit the microorganisms collection range.

  The invention according to claim 8 of the present invention is configured to perform the non-filtration treatment by coloring with a paint, and has the effect of improving the visibility of the liquid specimen injection position.

  The invention according to claim 9 of the present invention is configured such that the non-filtration treatment is performed by the water repellent treatment, and has an effect that the filterability can be improved.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

(Embodiment 1)
As shown in FIG. 1, the filtration range limiting method includes a filtration limiting unit 1, a filtration member 101 including a microorganism collection filter 103, a filter press 102, and a filter receiver 104.

  In FIG. 1, the filtration range limiting means 1 is formed inside a filter press and is a cylindrical structure that indicates the injection position of a liquid specimen containing microorganisms having an opening on the inside.

  Here, the cylindrical structure is a material such as plastic, metal, or ceramic, and is a material that does not deteriorate or soften the liquid specimen.

  In the above-described configuration, the cylindrical structure is limited in the position where the liquid sample is moved and filtered when the liquid sample is injected, and the liquid sample is rotated around the outer periphery of the filter 103 for collecting microorganisms. It can be limited.

  In addition, what is necessary is just to make it enter the inside of a filter holding | suppressing shape, and the shape of the part which limits the filtration range is not limited to a circle, a square, a triangle, etc.

(Embodiment 2)
FIG. 2 is a cross-sectional view showing an example of the filtration limiting means of the second embodiment. In FIG. 2, the filtration limiting means is configured by changing the cylindrical structure of the first embodiment into a tapered structure 2 provided with a taper having a large liquid sample inlet side and a small microorganism collecting side.

  In the above configuration, the angle of the contact portion between the tapered structure 2 and the filter surface for collecting microorganisms becomes obtuse, thereby reducing the surface tension of the liquid specimen remaining in the contact portion and improving the filtration performance. The filtration range can be limited.

  The taper position may not be continuous from the upper part of the opening, but only at the contact portion between the tubular structure and the filter surface for collecting microorganisms.

(Embodiment 3)
FIG. 3 is a cross-sectional view of the filtration limiting means showing an example of the third embodiment. The filtration range limiting means of FIG. 3 includes the cylindrical structure having a first liquid specimen injection means 3, a second liquid injecting means 4, and a third liquid injection means 5, and a first liquid specimen injection The means 3 is configured inside the second liquid specimen injecting means 4, the second liquid specimen injecting means 4 is configured inside the third liquid specimen injecting means 5, and the second liquid specimen injecting means 4. Is movable outside the first liquid specimen injecting means 3, and the second liquid specimen injecting means 4 and the first liquid specimen injecting means 3 are in contact with each other without any gap. 5 is movable outside the second liquid specimen injecting means 4, and the third liquid specimen injecting means 5 and the second liquid specimen injecting means 4 are in contact with each other without any gap. By pulling the means 5 upward, the height of the cylindrical structure 6 can be changed. It consists of.

  In the above configuration, by pulling the third liquid sample injection means 5 upward, the liquid sample injection volume of the filtration limiting means can be increased, and the amount of liquid sample that can be injected at one time can be increased. As a result, the filtration range can be limited.

  In addition, by performing water-repellent treatment such as a fluorine coating material on the structure shown in FIGS. 2 and 3, it is possible to eliminate the liquid specimen attached to the structure, thereby further improving the filterability. .

(Embodiment 4)
FIG. 4 is a diagram illustrating an example of an unfiltered process according to the fourth embodiment. In FIG. 4, an unfiltered process 7 is performed to prevent the outer periphery of the filter 103 for collecting microorganisms from being filtered.

The non-filtering process 7 is performed by applying an adhesive, applying and coloring a paint, a water repellent process or the like.
The material of the non-filtration treatment 7 does not deteriorate the microorganism collection filter 103. For example, the adhesive does not contain an organic solvent, the paint is water-based, and the water-repellent treatment uses a fluorine coating agent or the like.

  In the above configuration, since only the portion not subjected to the non-filtration treatment 7 can be filtered, microorganisms are not collected on the outer periphery of the microorganism collection filter 103, and the collection range of microorganisms can be limited.

  Moreover, when colored with a paint, the visibility of the liquid specimen injection position can be improved.

  Moreover, when the non-filtration process 7 is given by water-repellent treatment, the water drainage of the non-filtration process part can be improved and filterability can be improved.

  In addition, the same effect as the case where it colors with a coating material can be acquired by using the material which colored the adhesive agent and the water repellent treatment material for the non-filtration process 7. FIG.

  The method for limiting the range of collecting microorganisms of the present invention has the effect of accurately detecting microorganisms when measuring microorganisms in a liquid sample containing microorganisms using a filter for collecting microorganisms. It is useful as a filtration method for use in a rapid measurement apparatus for microorganisms.

Sectional drawing of an example of the microorganisms collection means of Embodiment 1 of this invention Sectional drawing of an example of the filtration range limitation means of Embodiment 2 of this invention Sectional drawing of an example of the filtration range limitation means of Embodiment 3 of this invention The figure of an example of the non-filtration process of Embodiment 4 of this invention Cross-sectional view of a conventional filtering member

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Filtration range limitation means 2 Tapered structure 3 1st liquid specimen injection means 4 2nd liquid specimen injection means 5 3rd liquid specimen injection means 6 Height variable structure 7 Unfiltering process 101 Filtration member 102 Filter holding | suppressing 103 Filter 104 Filter receiver

Claims (9)

In the method for collecting microorganisms by injecting and filtering a liquid specimen containing microorganisms into a filtration member constituted by a filter for collecting microorganisms, a filter receiver for holding the filter, and a filter holder for holding the filter, the filter A filtration range limiting means for limiting the filtration range of the liquid specimen inside the filter holder of the microorganism collection surface of the microorganism, and by collecting the filtration range of the liquid specimen, the collection range of microorganisms to be collected A method for collecting microorganisms, characterized in that The method for collecting microorganisms according to claim 1, wherein the filtering range limiting means of the filter is a cylindrical structure provided with an opening. The method for collecting microorganisms according to claim 2, wherein a tapered structure is provided by providing a taper inside the cylindrical structure so that the microorganism collection side is smaller than the liquid sample injection side. 4. The method of collecting microorganisms according to claim 2 and 3, wherein the height of the tubular structure and the tapered structure is variable. 5. The method for collecting microorganisms according to claim 2, wherein the tubular structure, the tapered structure, and the variable height structure are subjected to a water repellent treatment. The method for collecting microorganisms according to claim 1, wherein the means for limiting the filtration range of the filter is configured such that an unfiltered treatment that cannot be filtered is performed on the outer periphery of the microorganism collection surface of the filter. The microorganism collection method according to claim 6, wherein the non-filtration treatment is configured such that the outer periphery cannot be filtered by applying an adhesive to the outer periphery of the microorganism collection surface of the filter. The microorganism collection method according to claim 6, wherein the non-filtration treatment is configured such that the outer periphery cannot be filtered by coloring the outer periphery of the microorganism collection surface of the filter with a paint. The microorganism collection method according to claim 6, wherein the non-filtration treatment is configured such that the outer periphery of the microorganism collection surface of the filter is subjected to a water repellent treatment so that only the outer periphery cannot be filtered.

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