JP2005255884A - Wash liquid for probe for clinical test automatic analyzer and method for washing - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To make washing of a probe for collecting a specimen complete, to prevent contamination among specimens and to improve reliability of measured values when the specimens are continuously analyzed with a clinical test automatic analyzer. <P>SOLUTION: A wash liquid for the clinical test automatic analyzer containing a chaotropic ion, e.g. SCN<SP>-</SP>is used. The concentration of the chaotropic ion used as the wash liquid is at least ≥0.1 mol/L and the pH is ≥10. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a cleaning solution and a cleaning method for a probe for an automatic clinical analyzer. More specifically, the present invention relates to a cleaning solution and a cleaning method for the probe, which are effective for preventing contamination between samples.

  In the analysis by the clinical laboratory automatic analyzer, it is often required to intensively analyze a large number of specimens in a short time, and there is a concern about the occurrence of contamination between specimens for a specific clinical laboratory marker.

  That is, in the clinical laboratory automatic analyzer, the probe from which one sample is collected is washed after analysis and is used for collecting the next sample. There is a risk of contaminating the specimen, and particularly when the previous specimen contains a specific test marker at a high concentration, the subsequent specimen may be severely contaminated if the washing is not sufficient.

  Conventionally, in order to prevent such contamination between specimens, it has been actually practiced to wash the probe with an aqueous sodium hypochlorite solution instead of simply washing with water.

  However, when analyzing a large number of specimens in a short time in a situation where there are a wide variety of clinical test markers and the test sensitivity is very high, In the cleaning method, it was actually impossible to completely prevent contamination between samples.

  SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide a cleaning solution and a cleaning method for a probe for an automatic clinical testing apparatus, which can easily and reliably clean the probe and thereby prevent contamination between specimens. is there.

  Still other objects and advantages of the present invention will become apparent from the following description.

  According to the present invention, the above objects and advantages of the present invention are achieved firstly by a washing solution for a probe for an automatic analyzer for clinical testing, characterized in that it contains chaotropic ions.

  According to the present invention, the above-mentioned objects and advantages of the present invention are as follows. Secondly, in the method of collecting and measuring a specific clinical test marker with a clinical test automatic analyzer, the first sample is measured. Then, before measuring the second specimen, the probe from which the first specimen is collected is washed with a washing solution containing chaotropic ions. By the clinical laboratory marker measurement method using a clinical automatic analyzer, Achieved.

  According to the cleaning agent of the present invention, since the probe is reliably cleaned, contamination between samples can be prevented, so that the reliability of the measured values of the clinical laboratory markers analyzed for each sample continuously can be ensured. Can do.

The washing liquid of the present invention contains chaotropic ions. Anions and cations are known as chaotropic ions, but chaotropic anions are preferably used in the present invention. Preferred examples of the chaotropic anion include SCN ⁻ , I ⁻ , ClO ₄ ⁻ , NO ₃ ⁻ and Br ⁻ . As a compound which produces | generates this chaotropic anion in water, the sodium salt of the said anion, potassium salt, ammonium salt etc. can be mentioned as a preferable thing, for example.

  The concentration of chaotropic ions is preferably at least 0.1 mol / l, more preferably 0.5 to 4.0 mol / l, more preferably 0.8 to 1.5 mol / l. At concentrations lower than 0.1 mol / l, probe cleaning may not always be sufficient.

  The washing liquid of the present invention preferably has a pH of 10 or more, more preferably 12 or more, and particularly preferably 13 or more. Such pH adjustment can include alkaline compounds such as inorganic bases such as caustic soda and caustic potash or organic bases such as tetramethylammonium hydroxide.

  In an automatic clinical test analyzer, a sample is collected on a probe and a specific clinical test marker is measured for the sample. Examples of clinical laboratory markers include BNP, ANP, AFP, CEA, PSA, PLAII, and HBs antigen. In an automatic clinical test analyzer, analysis is performed as follows, for example. In a first reaction cup provided in the apparatus, a predetermined amount of human plasma (specimen) collected with a probe, a clinical test marker to be measured, for example, an antibody against a heart disease marker (antigen), for example, a biotin-labeled antibody and the clinical test For example, an ALP-labeled antibody is inserted as another antibody against the marker, and an antigen-antibody complex is produced in which a clinical test marker is sandwiched between a biotin-labeled antibody and an ALP-labeled antibody. Next, the immobilized anti-biotin antibody and the reaction solution in the first reaction cup are brought into contact with each other in the second reaction cup provided in the apparatus, and the immobilized anti-biotin antibody and the first reaction cup are brought into contact with each other. The produced antigen-antibody complex is reacted. Next, after washing the immobilized reaction product, a luminescent substrate is added and the amount of luminescence derived from the ALP label is measured. From the measured luminescence amount, the concentration of the clinical test marker to be measured is obtained using a calibration curve.

  The measurement for one sample is completed by the procedure as described above, but the probe used for collecting the sample is then washed to collect the next sample. The cleaning agent of this invention is used in that case. The probe washed with the washing agent is then washed with ion-exchanged water and then used for collecting the next specimen. According to such a procedure, analysis of samples is continuously performed one after another by the clinical laboratory analyzer. In actual cleaning, it is sufficient to suck the cleaning liquid into the probe and immediately drain it.

  In order to describe the present invention in more detail, examples and comparative examples will be described below, but the present invention is not limited to these examples.

Example 1
In order to confirm carryover (contamination between samples), BNP is selected as a measurement item, a high BNP concentration sample (specimen A, BNP concentration: 3833.9 pg / mL) as a sample that gives contamination, and low as a sample that checks contamination. A BNP concentration sample (Sample B, BNP concentration: 2.7 pg / mL) was selected. Using a fully automatic chemiluminescence immunoassay device MI02 (manufactured by A & T Co., Ltd.) as an automatic analyzer for clinical examination, the cleaning effect of the probe was performed on the cleaning solution of the present invention. As the washing liquid of the present invention, an aqueous solution composed of 1 mol / L sodium thiocyanate (manufactured by Wako Pure Chemical Industries, Ltd.) and 1N sodium hydroxide (manufactured by Wako Pure Chemical Industries, Ltd.) was used. Moreover, the actual washing operation was performed by automatically supplying the probe to the probe by setting the bottle containing the washing liquid in the clinical analyzer automatic analyzer.

  The results are shown in Table 1. As is clear from Table 1, even when the sample B was measured immediately after the sample A was measured, no contamination by the sample A was observed.

Examples 2-5 and Comparative Examples 1-12
As the washing liquid, the washing liquid described in Table 2 below was prepared.

  The washing solution was changed to each washing solution, and the BNP concentration of each specimen was measured in the same procedure as in Example 1. The results are shown in Table 3.

Claims (8)

A cleaning solution for a probe for an automatic analyzer for clinical examination, characterized by containing chaotropic ions. Chaotropic ions _{^{SCN -, I -, ClO 4}} -, NO 3 - or Br ^- cleaning solution according to claim 1 is. The washing solution according to claim 1 or 2, wherein the concentration of chaotropic ions is at least 0.1 mol / l. The washing liquid according to any one of claims 1 to 3, wherein the pH is 10 or more. In a method of collecting a specific clinical test marker by using a clinical test automatic analyzer and collecting a sample on a probe, the first sample is collected after measuring the first sample and before measuring the second sample. A method for measuring a clinical laboratory marker using a clinical automatic analyzer, wherein the probe is washed with a washing solution containing chaotropic ions. The method according to claim 5, wherein the chaotropic ion is SCN ⁻ , I ⁻ , ClO ₄ ⁻ , NO ₃ ⁻ or Br ⁻ . The method according to claim 5 or 6, wherein the concentration of chaotropic ions in the washing liquid is at least 0.1 mol / l. The method according to any one of claims 5 to 7, wherein the pH of the washing liquid is 10 or more.