JP2005249535A - Dispensation probe and autoanalyzer equipped therewith - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a dispense probe which is satisfactory in cleaning properties, without exerting influence on the dispensation precision. <P>SOLUTION: The dispense probe 17 for performing either suction or delivery of a liquid is equipped with a plurality of straight tube parts 30 which are parallel with the center axial line A and is arranged so as to stepwise become narrower for the inside diameter and a plurality of diameter contracted parts 31 for jointing the straight tube parts 30. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an automatic analyzer that performs various tests on a biological sample, and a dispenser that is suitable for use in a dispensing operation in the automatic analyzer.

In an automatic chemical analyzer, a reaction caused by dispensing a sample of human urine, blood, etc. into a reaction container, and dispensing and mixing a predetermined reagent set for each test item. The specimen is analyzed by detecting the state. A dispensing probe is used for dispensing samples such as specimens and reagents (hereinafter referred to as samples including specimens and reagents) into the reaction container. This dispensing probe is connected to a motor-driven syringe-type pump or the like, and is configured to suck and discharge a sample by the operation of the pump.
After such suction and discharge operations, a small amount of sample remains attached to the inner and outer surfaces of the dispensing probe. If the sample remaining in the dispensing probe is carried over and mixed with another sample, the analysis result will be affected. Therefore, whenever the sample is changed after aspiration or ejection, the inner and outer surfaces of the dispensing probe are changed. Is washed with a washing solution.

In this way, what is carried over to another sample is carried over when a dispensing probe is inserted into the sample cup in which the sample is placed when the sample is sucked (other samples are placed in the cup of another sample). Hereinafter, it is referred to as carry-over between specimens) and what is carried over into the reaction container when the sample is discharged (in the reaction container, a sample not originally used for an experiment used in another reaction is mixed). Hereinafter, it is referred to as carry-over between tests.).
On the other hand, in the field of automatic chemical analyzers, more sensitive measurement is performed, and further improvement in processing speed is desired. Therefore, in order to perform washing of the above-mentioned dispensing probe more efficiently in a shorter time and to prevent carry-over to another sample, the remaining amount of the sample used in the previous step attached to the dispensing probe is reduced. Therefore, there is a need for a dispensing probe with good detergency that can be washed to a low level.

As a method for improving the cleaning effect, a dispensing nozzle having a high cleaning degree while having a shape with a large cross-sectional area of the flow path has been proposed (for example, see Patent Document 1). In the dispensing nozzle described in Patent Document 1, a flat portion is molded into the dispensing nozzle, and the cleaning liquid that passes through the dispensing nozzle becomes a turbulent state in this flat portion to improve the cleaning performance. .
In addition, a pipette with a high degree of cleaning has been proposed in which an automatic analyzer is miniaturized (see, for example, Patent Document 2). The pipette described in Patent Document 2 dispenses a specimen, a reagent, and the like into a reactor, and is formed in a spiral shape from the middle to the tip of the pipette. Thereby, since the flow rate of the cleaning liquid passing through the dispensing probe is different between the inside and the outside, a turbulent state is caused, and the cleaning performance is improved.
Japanese Utility Model Publication No.2-2680 JP-A-62-262751

In the above conventional methods, the turbulent flow is generated by the specific shapes of the dispensing nozzle and the pipette to improve the cleaning performance. However, turbulent flow is a flow that has a direction toward the tip of the dispensing nozzle, and depending on the specific shape, a place where the attached sample tends to fall and a place where the attached sample tends to remain are formed. As a result, a great effect cannot be obtained. On the other hand, in the shape in which turbulent flow is likely to occur, the flow at the time of suction is also non-uniform, and it is difficult to obtain sufficient dispensing accuracy by generating bubbles and entraining them inside.
In addition, blood components include components that coagulate and adhere to blood, such as fibrin, which may cause clogging of the dispensing probe during use, and maintenance such as passing a mandrel is generally used. Has been made. However, the above prior art has a shape that does not allow the mandrel to pass, and maintenance using this cannot be performed. For this reason, it is difficult to use the same dispensing probe for a long period of time.

  The present invention has been made in view of the above-described circumstances, and an object of the present invention is to provide a dispensing probe with good cleaning properties without affecting dispensing accuracy.

In order to achieve the above object, the present invention provides the following means.
The dispensing probe according to the present invention is a dispensing probe that performs either one of liquid suction or discharge, and includes a plurality of straight pipe portions arranged so that the central axis is parallel and the inner diameter is gradually reduced. And a plurality of reduced diameter portions provided between the straight pipe portions.

  According to the present invention, since the reduced diameter portion is a specific portion of the dispensing probe, when a predetermined amount of liquid is dispensed, it becomes a portion where the liquid is more likely to adhere than the straight tube portion. However, during cleaning, the pressure of the cleaning liquid increases at the reduced diameter portion, and the inner diameter becomes narrower at the terminal end side (the distal end side of the dispensing probe) of the reduced diameter portion, so that the flow velocity increases. Thereby, the liquid accumulated in the reduced diameter portion is scraped out. Therefore, by providing the reduced diameter portion and the straight tube portion, a dispensing probe with high cleaning properties can be obtained.

In the dispensing probe of the present invention, it is preferable that the inclination angle of the inner peripheral surface of the reduced diameter portion is 10 ° to 45 ° with respect to the central axis of the straight pipe portion.
In the dispensing probe of the present invention, it is preferable that the reduction ratio of the inner diameter on one end side and the inner diameter on the other end side of the reduced diameter portion is 50% to 70%.

  According to the present invention, by appropriately setting the inclination angle of the reduced diameter portion and the inner diameter dimension of the reduced diameter portion, it is possible to obtain a dispensing probe with further improved cleaning performance.

  In addition, the dispensing probe of the present invention reduces the contraction so that the liquid level is positioned in the straight pipe portion even when any of the plurality of suction amounts preset based on the frequency of use is sucked. It is preferable that the position of the diameter portion is set.

  According to the present invention, the position of the reduced diameter portion is set according to a plurality of suction amounts set in advance based on the use frequency. That is, by designing a dispensing probe in consideration of a plurality of types of preset suction amounts, the number of reduced diameter portions where liquid remains after the dispensing probe sucks a predetermined suction amount is minimized. It becomes possible to stop. In general, the smaller the amount of suction, the larger the amount of liquid remaining after washing, and the more effective the smaller the dispensed amount.

The dispensing probe of the present invention preferably has a hydrophilic coating on the inner peripheral surface.
The dispensing probe of the present invention preferably has an antithrombotic coating on the inner peripheral surface.

  According to the present invention, when a predetermined amount of liquid is dispensed by applying a hydrophilic or antithrombotic coating to the inner peripheral surface of the dispensing probe, the liquid adhered to the inner peripheral surface of the dispensing probe. The amount can be reduced. That is, according to the hydrophilic coating, a film of water molecules can be formed on the surface to suppress the adhesion of the specimen, and according to the antithrombogenic coating, the coagulation adhesion between the liquid component and the surface can be suppressed. In addition, since the flow resistance decreases and the cleaning liquid flows easily on the surface coated with hydrophilic coating or antithrombogenic coating, the fluidity of the cleaning liquid during cleaning is improved, and the liquid adhering to the inner surface of the dispensing probe is removed. The ability to do is improved.

The analyzer according to the present invention includes an automatic analysis device including a reaction container into which a sample and a reagent are dispensed, and an analysis unit that inspects the sample from a mixed solution of the sample and the reagent mixed in the reaction container. In the apparatus, the dispensing probe according to any one of claims 1 to 6 is provided as dispensing means for dispensing the specimen and the reagent into the reaction container.
According to this invention, the inner surface of the dispensing probe can be effectively cleaned in a shorter time.

  According to the dispensing probe and the analyzer according to the present invention, by providing the straight tube portion and the reduced diameter portion, the pressure of the cleaning liquid increases in the reduced diameter portion, and the inner diameter becomes narrow at the end of the straight tube portion. The flow rate can be increased. Therefore, it is possible to efficiently scrape the liquid accumulated in the reduced diameter portion from the inside of the dispensing probe.

The automatic analyzer according to the first embodiment of the present invention will be described below with reference to FIGS.
As shown in FIG. 1, an automatic analyzer (analyzer) 1 according to this embodiment includes a reaction disk 2, a sample turntable 3, a reagent turntable 4, a sample dispensing unit ( Dispensing device) 8, reagent dispensing unit (dispensing device) 9, and the like are arranged.

  A plurality of reaction containers 5 are annularly arranged on the reaction disk 2 along a concentric circumference, and a plurality of sample containers 6 are arranged annularly along the concentric circumference on the sample turntable 3. It is installed. In addition, a plurality of reagent bottles 7 are annularly arranged on the reagent turntable 4 along a concentric circumference.

Each sample container 6 contains a sample to be analyzed, that is, blood, urine, fecal lysate, cultured cell solution, and the like, and each reagent bottle 7 has a plurality of types necessary for analysis items. Reagents are stored separately.
The reaction disk 2, the sample turntable 3, and the reagent turntable 4 can be intermittently rotated by a rotation mechanism (not shown) and positioned at predetermined positions. That is, in the reaction disk 2, the sample dispensing position P1, the reagent dispensing position P3, the stirring position P5, the measurement position P6, and the reaction container washing position P7 are set, and the positions are stored by an apparatus controller (not shown). The position is controlled. Similarly, a sample aspiration position P2 and a reagent aspiration position P4 are set in the sample turntable 3 and the reagent turntable 4, respectively.

Each of the sample dispensing unit 8 and the reagent dispensing unit 9 includes a dispensing probe 17 (described later). By a driving mechanism (not shown), the vertical movement in the vertical direction of the drawing and the rotation around the vertical axis of the drawing are performed. It can be rotated.
The sample dispensing unit 8 is arranged at a position where it can go back and forth between the sample dispensing position P1 on the reaction disk 2 and the sample suction position P2 on the sample turntable 3 by performing the rotating operation shown in FIG. It is installed. Further, the reagent dispensing unit 9 can move back and forth between the reagent dispensing position P3 on the reaction disk 2 and the reagent suction position P4 on the reagent turntable 4 by performing the rotating operation shown in FIG. It is arranged.

  The sample dispensing unit 8 is provided at a position between the sample dispensing position P1 and the sample aspiration position P2 on the path L1 through which the sample dispensing unit 8 passes by rotation. A specimen cleaning tank (cleaning device) 10 is provided for cleaning the outside of the dispensing probe 17 and discharging the cleaning liquid after cleaning the inside of the dispensing probe 17. Similarly, the reagent dispensing unit 9 is provided at a position on the path L2 between the reagent dispensing position P3 and the reagent suction position P4 and through which the reagent dispensing unit 9 passes by rotation. A reagent cleaning tank (cleaning device) 11 for cleaning the outside of the dispensing probe 17 and discharging the cleaning liquid after cleaning the inside of the dispensing probe 17 is provided.

  A stirring unit 12 is disposed at the outer peripheral position of the stirring position P5 so as to stir the liquid mixture in the reaction vessel 5 that has come to the position of the stirring position P5. A measurement unit 13 is disposed at the outer peripheral position of the measurement position P6, and the absorbance of the mixed solution in the reaction vessel 5 that has come to the position of the measurement position P6 is measured. A reaction container cleaning unit 14 is disposed at the outer peripheral position of the reaction container cleaning position P7, and the mixed liquid that has been measured and analyzed is discarded and the reaction container 5 is cleaned.

The overall operation of the automatic analyzer according to the present embodiment having the above-described configuration will be described below.
First, the respective dispensing probes 17 of the specimen dispensing unit 8 and the reagent dispensing unit 9 are moved to positions on the specimen washing tank 10 and the reagent washing tank 11, respectively. After stopping once at this position, the cleaning liquid is sent from the cleaning liquid supply pump to the tip of each dispensing probe. As a result, each of the dispensing probes is cleaned, and the inside of these dispensing probes and the pipe connected to the dispensing probe are filled with the cleaning liquid. This cleaning operation will be described in detail later.

Next, the dispensing probe of the sample dispensing unit 8 is moved to the sample aspirating position P2, and is further inserted into the sample container 6 at this position by lowering the dispensing probe. When the tip is lowered to a position several mm below the liquid level of the sample in the sample container 6, the operation of the dispensing probe is stopped, and then the syringe pump (not shown) is operated to suck the sample.
When the suction of a predetermined amount is completed, the dispensing probe is pulled up to a height that does not mechanically interfere with the sample container 6, and then moved to the sample dispensing position P1. After the movement is completed, the dispensing probe is lowered into the reaction container 5 installed at the sample dispensing position P1, and a predetermined amount of sample is dispensed by the operation of the syringe pump.

  After the completion of the dispensing, the dispensing probe is pulled up to a position where it does not interfere with the reaction vessel 5 and moved onto the specimen cleaning tank 10. When the position on the cleaning tank 10 is reached, the cleaning liquid supply pump operates to send the cleaning liquid to the dispensing probe. Thereby, the surplus specimen in the dispensing probe is poured out and the tip of the dispensing probe is washed. Thus, the dispensing of the sample into one reaction container 5 is completed. This cleaning operation will be described in detail later.

After completion of sample dispensing, the reaction disk 2 rotates and the reaction vessel 5 is sent to the reagent dispensing position P3. Even between the reagent dispensing position P3 and the reagent aspirating position P4, the dispensing unit 9 operates in the same manner as the dispensing unit 8, and the reagent is aspirated from the reagent aspirating position P4, and the sample enters at the reagent dispensing position P3. A reagent is dispensed into the reaction vessel 5.
After completing the dispensing of the reagent, the reaction disk 2 rotates and the reaction vessel 5 is sent to the stirring position P5. At the agitation position P5, the agitation unit 12 operates to agitate the sample and reagent mixture.

After stirring, after a predetermined time required for the sample and the reagent to react, the reaction vessel 5 is sent to the measurement position P6, and the change in absorbance at a predetermined wavelength corresponding to the analysis item is measured by the measurement unit 13. Analysis is performed.
After completion of the analysis, the reaction vessel 5 is sent to the reaction vessel washing position P7 and washed by the reaction vessel washing unit 14.
The above is a series of operations of the analysis work in the automatic analyzer of the present embodiment, and the work is repeatedly performed according to a preset analysis program.

  Subsequently, the details of the sample dispensing unit 8 and the reagent dispensing unit 9 will be described. Since the sample dispensing unit 8 and the reagent dispensing unit 9 have substantially the same configuration, they will be collectively described as “dispensing probe units” in the following description. In addition, the sample and the reagent that are to be dispensed are also simply referred to as “sample (liquid)” for explanation. This sample includes a biological sample or a liquid sample used for a biological test.

  As shown in FIG. 2, the dispensing probe unit of the present embodiment includes a dispensing probe 17 for sucking and discharging a sample. The dispensing probe 17 is supported via an arm 19 with respect to a support column 18 disposed coaxially with the Z-θ driving unit 22. The dispensing probe 17 can be moved in the height direction (Z-axis direction) and the rotation direction (θ direction) by driving the Z-θ drive unit 22.

As shown in FIG. 3, the dispensing probe 17 joins a plurality of straight portions (straight tube portions) 30 arranged so that the inner diameter becomes narrower toward the tip end 17 a and the straight portions 30. And a tapered portion (reduced diameter portion) 31 that is symmetrically inclined with respect to the central axis A of the portion 30.
The straight portion 30 includes a first straight portion 30a having a length of 10 mm and an inner diameter of 0.3 mm, a second straight portion 30b having a length of 10 mm and an inner diameter of 0.6 mm, and a first straight portion 30b having an inner diameter of 0.9 mm. 3 straight portions 30c, which are arranged in order from the tip 17a of the dispensing probe 17. And it arrange | positions so that the center axis line A of each straight part 30 may correspond substantially.

  The taper portion 31 includes a first taper portion 31a and a second taper portion 31b. The first taper portion 31a is disposed between the first straight portion 30a and the second straight portion 30b, and the second taper portion 31b includes the second straight portion 30b and the third straight portion 30c. The first straight portion 30a, the first taper portion 31a, the second straight portion 30b, the second taper portion 31b, and the third straight portion 30c are integrally formed, for example. ing. Further, the inclination angle of the inner peripheral surface of each taper portion 31 has a shape inclined by 20 ° with respect to the central axis A. Here, when the ratio of the amount of change from the inner diameter on one end side to the inner diameter on the other end side of each taper portion and the inner diameter on the one end side is defined as the diameter reduction rate, the diameter reduction rate of the first taper portion 31a is (Straight portion 30b-straight portion 30a) / straight portion 30b × 100 = (0.6−0.3) /0.6×100=50%. Regarding the second taper portion 31b, the diameter reduction ratio obtained by the same method is 33%.

The operation of the automatic analyzer 1 having the dispensing probe 17 described above will be described.
After the dispensing operation, the dispensing probe 17 moves to the specimen washing tank 10 or the reagent washing tank 11 and performs washing by passing a washing solution through the dispensing probe 17. When the dispensing probe 17 is washed, a sample is attached to the inner surface of each tapered portion 31 of the dispensing probe 17 as shown in FIG. Thus, the total amount of the sample adhered to the inner surface of the dispensing probe 17 having the two tapered portions 31 (the first tapered portion 31a and the second tapered portion 31b) is as shown in FIG. The number of taper portions (reduced diameter portions) 32 is smaller than that of a dispensing probe having only one location. At this time, when the diameter reduction rate is obtained in accordance with the above definition, the diameter reduction rate of the taper portion 31 is smaller than the diameter reduction rate of the taper portion 32. That is, fewer samples remain when the diameter reduction rate is smaller. Further, as shown in FIG. 5, when the horizontal axis is the pressure of the cleaning liquid applied to the taper portion 31 and the vertical axis is the amount of the sample remaining in the taper portion 31, when the pressure threshold becomes α or more, the taper portion 31 is disturbed. A flow state is formed and the sample is scraped.

  That is, according to the dispensing probe 17 according to the present embodiment, when the dispensing probe is cleaned, the sample is scraped in parallel at each tapered portion 31, so that the dispensing probe 17 having the tapered portion 32 only at one location is used. The sample is effectively removed. Further, by setting the water supply pressure of the cleaning liquid to be equal to or higher than the pressure threshold value α, the cleaning effect is improved, and the amount of adhered sample after cleaning is remarkably reduced.

  Further, by reducing the inclination angle of the taper portion 31, it is possible to generate a turbulent state with a lower pressure. The water supply pressure has a practical limit of about 0.3 to 0.4 Mpa because of durability of piping system components such as joints and solenoid valves. According to the present invention, by providing an appropriate number of taper portions 31 according to the sample, a turbulent flow state can be formed with a water supply pressure of 0.3 to 0.4 Mpa or less, and the cleaning effect can be improved. Accordingly, by forming the plurality of tapered portions 31, a dispensing probe with high cleaning properties can be obtained.

  In the present embodiment, the inclination angle of the inner peripheral surface of the tapered portion 31 is set to 20 ° with respect to the central axis A, but the same effect can be obtained in the range of 10 ° to 45 °. It is preferable that it is (degree) -30 degree.

Next, a dispensing probe according to a second embodiment of the present invention will be described with reference to FIG. In each embodiment described below, the same reference numerals are given to portions having the same configuration as the dispensing probe 17 and the automatic analyzer 1 according to the first embodiment described above, and the description will be omitted. .
In the dispensing probe 40 according to this embodiment, in addition to the dispensing probe 17 of the first embodiment, the straight portion 30 includes a fourth straight portion 30d, and the tapered portion 31 includes a third tapered portion 31c. The arrangement of the straight portion 30 and the tapered portion 31 is different from that of the first embodiment.

  The position of each tapered portion 31 is set so that the liquid level reaches the straight portion 30 even when any one of a plurality of dispensing amounts (aspiration amounts) set in advance based on the frequency of use is aspirated. Has been. That is, the position of the liquid surface in the dispensing probe 40 is accommodated in the straight portion 30 depending on the amount to be dispensed.

The operation of the automatic analyzer 1 having the dispensing probe 40 described above will be described.
Similarly to the first embodiment, when the syringe pump is operated and the sample is sucked into the dispensing probe 40, for example, the predetermined dispensing amount of the sample used in the dispensing devices 8 and 9 is 1 μL, 5 μL, When it is determined to be 10 μL and 25 μL, the dispensing probe 40 in which the taper portion 31 is provided between 1 μL to 5 μL, 5 μL to 10 μL, and 10 μL to 25 μL is used. That is, the sample is not immersed in the tapered portion 31 when dispensing 1 μL, the sample is immersed in the first tapered portion 31 a when dispensing 1 μL to 5 μL, and the first and second tapers are dispensed when dispensing 5 μL to 10 μL. The sample is immersed in the portions 31a and 31b, and the sample is immersed in the first, second, and third tapered portions 31a, 31b, and 31c when dispensing 10 μL to 25 μL. Then, after the completion of the dispensing, the dispensing probe 40 is cleaned as in the first embodiment.

  That is, according to the dispensing probe 40 according to the present embodiment, when the amount of sample to be used is 1 μL, the residue in the dispensing probe 40 can be removed almost completely, and when the amount is 1 μL to 5 μL, 10 μL to The residue can be suppressed to about 1/3 compared with 25 μL, and the residue can be suppressed to about 2/3 compared with 10 μL to 25 μL when compared with 10 μL to 25 μL. As a result, by using a dispensing probe in which the straight portion 30 and the taper portion 31 are arranged according to the dispensing amount, the sample remaining in the dispensing probe can be minimized, and the cleaning efficiency is improved. Can be made.

In each of the above embodiments, the reduced diameter portion is the tapered portion 31 whose inner diameter is gradually narrowed. However, for example, as shown in FIG. 7, the reduced diameter portion 51 of the dispensing probe 50 is located with respect to the central axis A. The structure which is not symmetrically inclined may be sufficient.
Further, by applying a hydrophilic coating or an antithrombogenic coating to the inner surface of the dispensing probes 17 and 40, a sample adhesion preventing effect can be obtained, and the flow resistance of the cleaning liquid is improved by reducing the channel resistance. In addition, the removal effect of the adhered sample is enhanced. Thereby, the remaining amount of the sample after washing can be reduced to a fraction of the number of the non-coated dispensing probes 17 and 40 or less.

  Moreover, the base material of the dispensing probe is SUS or titanium, and any coating agent can form a film satisfactorily. Furthermore, anti-thrombogenic coatings have the effect of inhibiting coagulation and adhesion of fibrin and the effect of dissolving the coagulated fibrin, eliminating the need to perform the mandrel itself and maintaining the dispensing probe. It can be simplified. The same effect can be obtained even when the dispensing probes 17 and 40 of the present invention are formed of hydrophilic alumina, polyurethane having antithrombotic properties, cardiosan, or the like. Furthermore, in a dispensing probe made of a polymer material such as nylon, polychlorinated resin, or fluororesin, urokinase can be immobilized to improve antithrombogenicity. Since urokinase is a fibrinolytic enzyme, further antithrombogenicity can be obtained by dissolving the coagulated fibrin, and the adhesion and residue of the sample can be further reduced. In addition, heparin is also exemplified as a material that has such an antithrombotic property and can be immobilized on a polymer material, and similar effects can be obtained.

  The technical scope of the present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the spirit of the present invention.

It is a top view which shows the automatic analyzer which concerns on 1st Embodiment of this invention. It is a top view which shows the dispensing probe which concerns on 1st Embodiment of this invention. It is an enlarged plan view which shows the dispensing probe which concerns on 1st Embodiment of this invention. It is sectional drawing which shows the sample adhering to the inner surface of the dispensing probe which concerns on 1st Embodiment of this invention. It is a figure which shows the relationship between the pressure of the washing | cleaning liquid in the automatic analyzer which concerns on 1st Embodiment of this invention, and the quantity of the sample which remains on a dispensing probe. It is an enlarged plan view which shows the dispensing probe which concerns on 2nd Embodiment of this invention. It is an enlarged plan view which shows the other example of the dispensing probe which concerns on each embodiment of this invention.

Explanation of symbols

A Center axis 1 Automatic analyzer 5 Reaction vessel 17, 40, 50 Dispensing probe 30 Straight section (straight pipe section)
31, 32 Tapered part (reduced diameter part)

Claims (7)

In a dispensing probe that performs at least one of liquid suction and discharge,
A plurality of straight pipe portions arranged such that the central axis is parallel and the inner diameter is gradually reduced;
A dispensing probe comprising a plurality of reduced diameter portions provided between the straight pipe portions.   2. The dispensing probe according to claim 1, wherein an inclination angle of an inner peripheral surface of the reduced diameter portion is 10 ° to 45 ° with respect to a central axis of the straight pipe portion.   2. The dispensing probe according to claim 1, wherein a reduction ratio between an inner diameter on one end side and an inner diameter on the other end side of the reduced diameter portion is 50% to 70%.   The position of the reduced diameter portion is set so that the liquid level is positioned in the straight pipe portion even when any of the suction amounts among a plurality of suction amounts set in advance based on the frequency of use is sucked. The dispensing probe according to any one of claims 1 to 3, wherein:   The dispensing probe according to any one of claims 1 to 4, wherein the inner peripheral surface is provided with a hydrophilic coating.   The dispensing probe according to any one of claims 1 to 4, wherein the inner peripheral surface is provided with an antithrombogenic coating. A reaction container into which a sample and a reagent are dispensed;
In an automatic analyzer comprising an analyzing means for inspecting the sample from a mixed solution of the sample and the reagent mixed in the reaction container,
An analyzer comprising the dispensing probe according to any one of claims 1 to 6, as dispensing means for dispensing the sample and the reagent into the reaction container.

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