JP2005232083A - Therapeutic agent for hepatic failure and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a therapeutic agent exhibiting excellent curative effects on various kinds of hepatic failure, and hardly causing side effects even after administration over a long period; and to provide a method for producing the therapeutic agent. <P>SOLUTION: A saponin of Kan'oso is obtained in high yield by inserting a powder of a root vegetable of the Kan'oso (dried Dendrobium officinale K. Kimura et Makino) wrapped with filter paper in a solvent flask of a Soxhlet extractor with an alcohol, refluxing the alcohol until the solution becomes colorless, recovering the alcohol, adding another kind of alcohol to the solution after recovering the alcohol, refluxing the resultant mixture, removing the alcohol under a reduced pressure therefrom, and drying the residue by using anhydrous sodium sulfate. The high curative effects are recognized by administering the obtained saponin of the Kan'oso to a patient suffering from the hepatic failure. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a therapeutic agent for liver disease, and particularly relates to a therapeutic agent that exhibits a remarkable curative effect on chronic liver disease and does not cause side effects even when taken over a long period of time. is there.

  The liver is an important organ with various functions such as detoxification, various metabolisms, and nutrient storage, but it is damaged by various causes such as viruses, drugs, alcohol, etc., and acute hepatitis, chronic hepatitis, fatty liver, etc. May eventually lead to cirrhosis. In particular, chronic hepatitis and cirrhosis with mesenchymal reactions such as cell infiltration and liver fibrosis are known as intractable diseases. Conventionally, steroids, immunosuppressants such as cyclosporine, glucogan insulin, interferon, and the like are known as therapeutic agents for such liver disorders (Patent Document 1).

Japanese translation of PCT publication No. 2002-531397

  However, although the above-mentioned conventional therapeutic agents can exhibit a certain degree of curative effect, there is a possibility that side effects may occur when taken over a long period of time, so that they are effective for patients with chronic liver diseases. It's not the right one. An object of the present invention is to solve the above-mentioned problems of conventional therapeutic agents, have a remarkable curative effect on various liver diseases, and have no side effects even when taken over a long period of time, and It is in providing the manufacturing method.

  Among the present inventions, the constitution of the invention described in claim 1 resides in a therapeutic agent for liver diseases comprising saponin extracted from roots of corn yellow grass as an active ingredient. Kang yellow grass is a plant that grows in the outback of Guangxi and Sichuan, China.

  The structure of the invention described in claim 2 is that the first alcohol is collected after dissolving the root vegetable powder of corn yellow grass in the first alcohol, and then the residue is different from that of the first alcohol. It exists in the manufacturing method of the therapeutic agent of a liver disease including the process of collect | recovering the said 2nd alcohol after making it melt | dissolve in a 2nd alcohol.

  According to a third aspect of the present invention, the alcohol is recovered after dissolving the root vegetable powder of corn yellow grass in alcohol, and then the precipitate is recovered from the liquid obtained by adding acetone to the solution after the alcohol recovery. A method for producing a therapeutic agent for liver disease comprising the step of:

  According to a fourth aspect of the present invention, there is provided a method for producing a therapeutic agent for liver disease, comprising the step of boiling roots of corn yellow grass with water and then filtering the solution after the boiling and collecting the filtrate.

  The therapeutic agent for liver disease according to claim 1 effectively reduces the levels of ALT, AST and bilirubin in patients with liver diseases such as acute hepatitis, childhood acute jaundice hepatitis, chronic toxic hepatitis, hepatitis C and hepatitis B And can exhibit a high healing effect. It is also possible to prevent HBV-DNA replication in hepatitis patients. And even if it takes for a long time, a side effect does not arise. In addition, even when administered to patients whose sugar intake is restricted, there is no adverse effect.

  According to the method for producing a therapeutic agent for liver disease described in claims 2 to 4, saponin can be extracted from can yellow grass with a high yield. It can be manufactured inexpensively and efficiently.

  Hereinafter, the therapeutic agent for liver disease and the method for producing the same according to the present invention will be described in detail with reference to the drawings.

[Extracted form 1 of can yellow grass saponin]
The collected roots of corn yellow grass were dried and then finely pulverized to obtain a root vegetable powder of corn yellow grass. Then, 5 g of the rooted vegetable powder of kan yellow grass obtained by wrapping with filter paper is put into a solvent flask of a Soxhlet extractor together with 200 ml of 80% concentration methanol, and it takes about 8 hours until the solution becomes colorless. Circulated. Thereafter, methanol was recovered, and 50 ml of 50% n-butyl alcohol was added to the recovered solution and circulated three times. Then, n-butyl alcohol was removed under reduced pressure, 1 g of anhydrous sodium sulfate (Na ₂ SO ₄ ) was added, and the residue was dried to obtain about 0.6835 g of corn saponin (in addition, FIG. 1 illustrates the above extraction method). The yield was 13.6%.

[Extraction Form 2 of Can Yellow Grass Saponin]
The roots of the collected corn yellow grass were dried and pulverized to obtain 20 mesh corn yellow grass root vegetable powder. Then, 5 g of the obtained corn yellow grass root vegetable powder wrapped with filter paper was put into a solvent flask of a Soxhlet extractor together with 200 ml of 45% concentration of petroleum ether for defatting. Thereafter, 200 ml of ethanol having a concentration of 95% was put into the solvent flask of the Soxhlet extractor and circulated for about 8 hours until the solution became colorless. Further, ethanol was recovered, and a precipitate was recovered by filtering a liquid containing 50 ml of 50% acetone in the recovered aqueous solution. Thereafter, the precipitate was washed with 20 ml of acetone to obtain about 0.6555 g of corn saponin (note that FIG. 2 illustrates the above extraction method). The yield was 13.21%.

[Extracted form 3 of corn yellow saponin]
The roots of corn yellow grass immediately after collection were dried and pulverized to obtain root vegetables powder of corn yellow grass. Then, 10 kg of water containing no ions was added to 5 kg of the root beetroot powder of corn yellow grass thus obtained and boiled for about 10 minutes, and then the liquid was filtered to collect the filtrate. Furthermore, a series of operations of adding 10 kg of water not containing ions to the filtered residue and boiling for about 10 minutes and then filtering the liquid and collecting the filtrate was repeated twice. Then, the filtrate obtained three times was mixed and heated to evaporate the water to obtain about 0.125 kg of corn saponin (note that FIG. 3 illustrates the above extraction method). Is). The yield was 2.5%.

[Clinical result 1]
When kanpon grass saponin was administered to acute hepatitis patients (189) for 3 months, 93.65% of patients had curative effects and 6.35% had significant healing. The effect was recognized. In addition, when Kang yellow grass saponin was continuously administered to patients with acute hepatitis (54 patients) for 3 months, as shown in Table 1, 96.30% of patients had a curative effect, and 3.70% A significant curative effect was observed in these patients (see Table 1 below). In addition, no side effects were observed in patients who received saponin of corn yellow grass.

[Clinical result 2]
When kanpon grass saponin was continuously administered to chronic toxic hepatitis patients (104) for 3 months, a remarkable curative effect was observed in 80.76% of the patients. In addition, when Kang yellow grass saponin was continuously administered to patients with hepatitis C (12 people) over 3 months, a remarkable curative effect was observed in 50.00% of patients. Furthermore, when Kang yellow grass saponin was continuously administered to patients with hepatitis B (155 patients) over 3 months, 36.12% of the patients had a remarkable curative effect (see Table 2 below). ). In addition, no side effects were observed in patients who received saponin of corn yellow grass.

[Clinical result 3]
Table 3 shows changes in the ALT value (average value) when kanpon grass saponin was continuously administered to chronic hepatitis patients (30 people) over 3 months. For comparison, Table 3 also shows the change in the ALT value (average value) when Silibinin-N-Meflylucamine was continuously administered to another chronic hepatitis patient (30 people). There were no side effects observed in patients who received saponin of corn yellow grass. From Table 3, it can be seen that when the saponin of corn yellow grass is continuously administered (“Gansu” in Table 3), the degree of decrease in the ALT value is greater than when silibinin-N-Meflylucamine is continuously administered. .

[Clinical result 4]
Table 4 shows the change in G value (average value) when saponin of can yellow grass was continuously administered to patients with chronic hepatitis B (25 people) over 3 months. For comparison, Table 4 also shows changes in the G value (average value) when Yiganling and carmine were continuously administered to another chronic hepatitis B patient (20 patients). There were no side effects observed in patients who received saponin of corn yellow grass. From Table 4, it can be seen that the degree of decrease in the G value is greater when kanpon grass saponin is continuously administered than when Yiganling and carmine are continuously administered.

[Clinical result 5]
Table 5 shows the decrease rate (average value) of HBV-DNA after kanpon grass saponin was continuously administered to various hepatitis patients (155 patients) over 3 months. For comparison, Table 5 also shows the reduction rate (average value) of HBV-DNA after continuing to administer Jitaile and carmine to another hepatitis patient (60 people). There were no side effects observed in patients who received saponin of corn yellow grass. From Table 5, it can be seen that the degree of decrease in HBV-DNA is greater when kanpon grass saponin continues to be administered than when Jitaile and carmine continue to be administered.

  In addition, the structure of the therapeutic agent of this invention and its manufacturing method is not limited to the aspect of the said embodiment at all, and can be suitably changed in the range which does not deviate from the meaning of this invention. For example, can yellow grass saponin can be extracted by an extraction method other than the above embodiment. Moreover, when each said extraction method is employ | adopted, it is also possible to change extraction time and the density | concentration of a solvent as needed. Furthermore, in the case of extracting corn saponin in an embodiment like the extraction form 1, ethanol can be used instead of methanol as shown in FIG. In addition, the therapeutic agent of the present invention is not limited to one containing only can yellow grass saponin, and may contain different components.

  Since the corn saponin according to the present invention has a high curative effect as described above and does not cause side effects, it can be widely applied as a therapeutic agent for liver diseases.

It is explanatory drawing which shows the extraction method of can yellow grass saponin. It is explanatory drawing which shows the extraction method of can yellow grass saponin. It is explanatory drawing which shows the extraction method of can yellow grass saponin. It is explanatory drawing which shows the extraction method of can yellow grass saponin.

Claims (4)

  A therapeutic agent for liver disease, characterized by comprising saponin extracted from roots of citrus yellow grass as an active ingredient.   The first alcohol is recovered after dissolving corn yellow root powder in the first alcohol, and then the residue is dissolved in the second alcohol different from the first alcohol, and then the second alcohol is recovered. The manufacturing method of the therapeutic agent of a liver disease characterized by including the process of collect | recovering alcohol of this.   Treatment of liver disease, comprising the steps of: recovering the alcohol after dissolving corn yellow grass root vegetable powder in alcohol; and then recovering the precipitate from a liquid obtained by adding acetone to the solution after the alcohol recovery Drug manufacturing method. A method for producing a therapeutic agent for a liver disease, comprising the step of boiling corn yellow root powder with water and then filtering the boiled solution and collecting the filtrate.

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