JP2005128030A5 - - Google Patents
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- JP2005128030A5 JP2005128030A5 JP2004377665A JP2004377665A JP2005128030A5 JP 2005128030 A5 JP2005128030 A5 JP 2005128030A5 JP 2004377665 A JP2004377665 A JP 2004377665A JP 2004377665 A JP2004377665 A JP 2004377665A JP 2005128030 A5 JP2005128030 A5 JP 2005128030A5
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- mobile phase
- liquid
- sample
- switching valve
- flow path
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- 239000007788 liquid Substances 0.000 claims 21
- 238000004458 analytical method Methods 0.000 claims 6
- 238000005070 sampling Methods 0.000 claims 6
- 238000000926 separation method Methods 0.000 claims 4
- 238000004811 liquid chromatography Methods 0.000 claims 2
Claims (2)
第１の移動相を送液する送液ポンプと分析用の分離カラムとを連結してなる流路において、前記送液ポンプと分離カラムとの間に試料及び第２の移動相を前記流路に導くためのインジェクタを接続してなり、前記インジェクタは、試料又は第２の移動相を吸引するサンプリングノズルと、前記サンプリングノズルに連結された切り換え弁と、前記切り換え弁に両端が連結されており、かつ、試料又は第２の移動相を保持する液体保持流路とを有し、前記切り換え弁は、第１の移動相が前記流路に流され、かつ、前記試料又は第２の移動相が前記液体保持流路に導かれる第１の切り換え状態と、第１の移動相を前記液体保持流路に導入し、かつ、前記試料又は第２の移動相を前記液体保持流路から前記流路に導く第２の切り換え状態との間で切り換えられるように構成されている液体クロマトグラフを用いるものであり、
前記切り換え弁を前記第１の状態と第２の状態とで切り換えることにより、第１の移動相から第２の移動相に、ステップ的に移動相を切り換えるものである
ことを特徴とする液体クロマトグラフによる分析方法。 An analysis method by liquid chromatography using a plurality of mobile phases consisting of at least a first mobile phase and a second mobile phase,
In a flow path formed by connecting a liquid feed pump for feeding a first mobile phase and a separation column for analysis, a sample and a second mobile phase are placed between the liquid feed pump and the separation column in the flow path. The injector has a sampling nozzle for sucking the sample or the second mobile phase, a switching valve connected to the sampling nozzle, and both ends connected to the switching valve. And a liquid holding channel for holding the sample or the second mobile phase, and the switching valve is configured such that the first mobile phase is caused to flow through the channel and the sample or the second mobile phase. Is introduced into the liquid holding channel, the first mobile phase is introduced into the liquid holding channel, and the sample or the second mobile phase is introduced into the liquid holding channel from the liquid holding channel. Switch between the second switching state leading to the road It is those using a liquid chromatograph is configured to be replaced,
A liquid chromatograph characterized in that the mobile phase is switched stepwise from the first mobile phase to the second mobile phase by switching the switching valve between the first state and the second state. Analysis method by graph.
第１の移動相を送液する送液ポンプと分析用の分離カラムとを連結してなる流路において、前記送液ポンプと分離カラムとの間に試料及び第２の移動相を前記流路に導くためのインジェクタを接続してなり、前記インジェクタは、試料又は第２の移動相を吸引するサンプリングノズルと、前記サンプリングノズルに連結された切り換え弁と、前記切り換え弁に両端が連結されており、かつ、試料又は第２の移動相を保持する液体保持流路とを有する液体クロマトグラフを用いるものであり、
少なくとも、
前記切り換え弁を、第１の移動相が前記流路に流され、かつ、前記インジェクタのサンプリングノズルから吸引した試料が前記液体保持流路に導かれる第１の切り換え状態とするステップ１と、
前記切り換え弁を、第１の移動相を前記液体保持流路に導入し、かつ、試料を前記液体保持流路から前記流路に導く第２の切り換え状態に切り換えるステップ２と、
前記液体保持用配管から試料が全て押し出された後に、切り換え弁９を、第１の移動相が前記流路に流され、かつ、インジェクタのサンプリングノズルから吸引した第２の移動相を前記液体保持流路に導く第１の切り換え状態に切り換えるステップ３と、
前記切り換え弁を、第１の移動相を前記液体保持流路に導入し、かつ、第２の移動相を前記液体保持流路から前記流路に導く第２の切り換え状態に切り換えるステップ４とを有する
ことを特徴とする液体クロマトグラフによる分析方法。 An analysis method by liquid chromatography using a plurality of mobile phases consisting of at least a first mobile phase and a second mobile phase,
In a flow path formed by connecting a liquid feed pump for feeding a first mobile phase and a separation column for analysis, a sample and a second mobile phase are placed between the liquid feed pump and the separation column in the flow path. The injector is connected to a sampling nozzle for sucking the sample or the second mobile phase, a switching valve connected to the sampling nozzle, and both ends connected to the switching valve. And using a liquid chromatograph having a liquid holding channel for holding a sample or a second mobile phase,
at least,
Setting the switching valve to a first switching state in which a first mobile phase is caused to flow through the flow path and a sample sucked from a sampling nozzle of the injector is guided to the liquid holding flow path;
Step 2 for introducing the switching valve into a second switching state for introducing a first mobile phase into the liquid holding channel and guiding a sample from the liquid holding channel to the channel;
After all of the sample has been pushed out from the liquid holding pipe, the switching valve 9 is used to hold the liquid in the second mobile phase that the first mobile phase has flowed through the flow path and sucked from the sampling nozzle of the injector. Step 3 for switching to the first switching state leading to the flow path;
Step 4 of introducing the switching valve into a second switching state for introducing the first mobile phase into the liquid holding channel and guiding the second mobile phase from the liquid holding channel to the channel. An analysis method using a liquid chromatograph, comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004377665A JP2005128030A (en) | 2004-12-27 | 2004-12-27 | Liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004377665A JP2005128030A (en) | 2004-12-27 | 2004-12-27 | Liquid chromatograph |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7487394A Division JPH07280789A (en) | 1994-04-13 | 1994-04-13 | Liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005128030A JP2005128030A (en) | 2005-05-19 |
| JP2005128030A5 true JP2005128030A5 (en) | 2005-08-18 |
Family
ID=34651062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004377665A Withdrawn JP2005128030A (en) | 2004-12-27 | 2004-12-27 | Liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2005128030A (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090126467A1 (en) | 2006-09-06 | 2009-05-21 | Thermo Electron S.P.A. | Washing device for liquid chromatography injectors |
| WO2012058516A1 (en) | 2010-10-29 | 2012-05-03 | Thermo Finnigan Llc | Method and system for liquid chromatograph with compressibility and viscosity monitoring to identify fluids |
| WO2013062624A1 (en) * | 2011-10-28 | 2013-05-02 | Thermo Finnigan Llc | Method and system for liquid chromatography fluidic monitoring |
| US10054569B2 (en) | 2010-10-29 | 2018-08-21 | Thermo Finnigan Llc | Method and system for liquid chromatography fluidic monitoring |
| US11262334B2 (en) | 2010-10-29 | 2022-03-01 | Thermo Finnigan Llc | Methods for liquid chromatography fluidic monitoring |
| CN104458978B (en) * | 2014-10-24 | 2016-01-13 | 北京佰纯润宇生物科技有限公司 | A kind of five eight logical valves and a kind of tomographic system based on five eight logical valves |
| JP6911940B2 (en) * | 2017-12-04 | 2021-07-28 | 株式会社島津製作所 | Liquid chromatograph |
-
2004
- 2004-12-27 JP JP2004377665A patent/JP2005128030A/en not_active Withdrawn
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