JP2005098790A - Chip for inspection - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a chip made of resin for inspection having a stable performance, at low cost. <P>SOLUTION: A bottom plate member 7 and a lid member 11 are each made of a biaxially-stretched PET plate, and its surface is made hydrophilic by a surface active agent. Since the upper and lower wall surfaces forming a reaction chamber 15 are made of the biaxially-stretched resin plate having hydrophilicity, it is possible to stabilize the flow of a liquid to be inspected and acquire inspection results with high reliability on the basis of this. In addition, the cost of the chip for inspection can be reduced, by using the resin plate easily acquirable as a constituent member. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an inspection chip for an aqueous liquid having a flow path.

  Conventionally, as a method for measuring various components contained in blood, a sample such as blood is flowed into a flow path having a fine cross-sectional area using capillary action or electrophoresis, and reacted with a reagent. There is a method in which each component is separated and subjected to transmission spectroscopic analysis, or a luminescence reaction with a reagent is performed to spectrally analyze the emitted light. For the purpose of performing such blood analysis more easily and with a small amount of sample, there has been proposed an apparatus that is miniaturized in a chip shape (for example, see Patent Document 1 and Patent Document 2).

  This device combines, for example, a container part having a groove structure and a flat lid part, and the contact surface thereof is liquid-tightly attached with, for example, a double-sided adhesive tape having adhesiveness on both sides or a self-adhesive sheet. It is a combination.

As a function, a space consisting of a groove serves as a reaction chamber, in which a liquid to be tested such as blood or serum is allowed to flow, and a reagent is introduced from a through hole connected to a flow path provided in a flat lid member. Blood components by reacting the liquid to be tested with the reagent in advance, or by applying the reagent to the inner surface of the flow path in advance, and testing the reacted liquid that flows out to the measurement part at the end of the flow path The state is determined.
Japanese Patent Laid-Open No. 8-294439 JP 2002-267777 A JP-A-8-145980

However, the conventional example having such a configuration has the following problems.
That is, as the material of the conventional inspection chip, a resin-made material is used to some extent, but generally an expensive glass-made material is often used. This is because the resin-made one has a problem that the variation in the inspection result is large and the reliability is low due to the fact that the flow state of the liquid to be inspected in the flow path is not stable as compared with the glass-made one. That is, as a problem of the resin inspection chip, it is necessary to stabilize the reaction by improving the flow state.

  For the purpose of improving the characteristics of such a resin testing chip, it has been proposed to apply a silicon oxide film or a cellulose derivative coating to the wall surface of the flow path to impart hydrophilicity to the wall surface (for example, Patent Documents). 3). However, even in the proposed method, stabilization of the flow is still not practical enough. In addition, there is a problem that the cost of forming a hydrophilic film such as a silicon oxide film is high.

  The present invention has been made in view of such circumstances, and an object of the present invention is to provide a resin inspection chip that is low in cost and stable in performance.

In order to achieve such an object, the present invention has the following configuration.
That is, the invention according to claim 1 is a test chip for performing component analysis of a sample solution that is a specimen through a reaction chamber, and at least one of the wall surfaces forming the reaction chamber is hydrophilic. And a biaxially stretched resin plate.

  [Operation / Effect] As a result of various studies on factors affecting the fluidity of the test target liquid, the inventor has found that the reaction chamber in contact with the test target liquid can be stabilized in order to stabilize the fluidity of the test target liquid. It has been found that it is effective that the wall surface is hydrophilic and flat. In other words, if either hydrophilicity or flatness is lacking, the flow of the liquid to be inspected will be insufficiently stabilized and the test results will vary, whereas if both are satisfied In addition, fluidity is stable and highly reliable test results can be obtained.

  A method for forming a highly flat wall surface at low cost is to use a resin plate with excellent surface flatness, but the fluidity of the liquid to be inspected can be greatly affected by the resin plate manufacturing method. I understood. Resin plates, which are general grades, are manufactured by methods such as extrusion molding and calender rolls. However, when resin plates made by these methods were used, the inspection results varied greatly. This is considered to be due to the fact that the surface of the resin plate produced by extrusion molding has a relatively large unevenness although it is a level that cannot be seen with the naked eye, and the fluidity is unstable. . On the other hand, it was found that when a resin plate produced by extrusion molding or the like is further stretched by biaxial stretching, the fluidity is stabilized and the reliability of the inspection result is increased. In the case of biaxial stretching, it is considered that the unevenness on the surface of the resin plate is reduced and the flatness is high.

  From this, it was found that a biaxially stretched resin plate is suitable as a material for the wall surface of the reaction chamber.

Next, a method for hydrophilizing the resin plate will be described.
As a method for hydrophilizing the resin, there is a method of forming a hydrophilic film such as a silicon oxide film, a cellulose derivative, or PVA on the surface as described above. As a more appropriate and low-cost method, a surfactant is used. It was found that hydrophilization by is effective. This is because in the case of having a hydrophilic film, since the hydrophilic film is in direct contact with the liquid to be inspected, the performance of the inspection chip is greatly affected by the flatness as in the case of the resin plate.

  That is, when a film such as a silicon oxide film, a cellulose derivative, or PVA is formed on the reaction chamber wall surface, the hydrophilicity is improved, but the surface smoothness is decreased. On the other hand, in the case of a hydrophilic treatment with a surfactant, the surface smoothness is hardly lowered. Specific methods of hydrophilization treatment with a surfactant include a method of applying a surfactant to the surface of a resin plate, or forming a plate in which a surfactant is kneaded into a resin. .

  Various materials can be used as the material of the resin plate as long as they can be stretched. However, the shape stability is good, the workability is high, and most importantly, the surface of the resin plate after stretching is used. The condition of high flatness is preferably satisfied, and for example, a polyester resin is suitable.

  An advantage of the present invention is that the optimal resin plate having these characteristics is a grade of a biaxially stretched polyester resin sheet having antistatic properties (hydrophilic surfactant), and is a mass-produced product from various resin manufacturers. And is easily available at low cost.

  In each invention, the reaction chamber is a space formed by laminating a container part having a concave structure and a flat lid part, and the container part constituting the upper and lower surfaces of the reaction chamber and / or It is preferable that the lid component is subjected to a hydrophilic treatment (claim 2).

  Moreover, it is preferable that the container part is provided with the flat frame part which has the penetrated opening part, and the flat bottom part which obstruct | occludes the one end side of the said opening part (Claim 3).

  Furthermore, the reaction chamber is a space formed by a flat lid component, a double-sided adhesive member having a penetrating opening, and a flat bottom component that closes one end of the opening, and the reaction chamber It is preferable that a hydrophilic treatment is applied to the lid part and / or the bottom part constituting the upper and lower surfaces (Claim 4).

  Moreover, it is preferable that hydrophilic treatment is applied to the parts constituting the side surface of the reaction chamber.

  According to the inspection chip according to the present invention, since at least one of the wall surfaces forming the reaction chamber is a resin plate that is hydrophilic and biaxially stretched, the flow of the liquid to be inspected is stabilized and based on this. A highly reliable test result can be obtained. Moreover, the cost of the inspection chip can be reduced by using an easily available resin plate as a component.

Embodiments of the present invention will be described below with reference to the drawings.
FIG. 1 shows a schematic configuration of an inspection chip according to an embodiment, where (a) is an exploded perspective view and (b) is a perspective view in an assembled state.

First, a schematic configuration of the inspection chip 1 according to the present embodiment will be described.
As shown in FIG. 1A, the inspection chip 1 includes, for example, a flat frame part 5 having a through opening 3 and a flat bottom part 7 that closes one end of the opening 3. And a lid part 11 having an opening 9 which is opened for dropping a sample liquid or the like and communicates with the opening 3. The frame part 5 and the bottom part 7 constitute a container part 13 having a recessed structure. Although not shown in the drawings, these abutting surfaces may be liquid-tightly bonded with, for example, a double-sided adhesive tape having adhesiveness on both sides or a self-adhesive sheet, or the frame part 5 itself may be attached. You may comprise a double-sided adhesive tape or a self-adhesive sheet.

  In addition, the code | symbol 11a of the lid | cover component 11 is an inlet of a test object liquid (sample liquid), the code | symbol 11b is an inlet of a reagent, and the code | symbol 11c is a measurement part.

  As shown in FIG. 1B, when the inspection chip 1 is assembled, a reaction chamber 15 is formed. A liquid to be tested, such as blood or serum, is allowed to flow into the test liquid inlet 11a, a reagent is supplied from the reagent input 11b, or a reagent is applied to the inner surface of the reaction chamber 15 in advance. Thus, the state of the blood component is determined by reacting the liquid to be tested with the reagent and inspecting the reacted liquid that flows out to the measurement unit 11c corresponding to the remaining opening 9.

  Next, the longitudinal cross-sectional structure of the above-described inspection chip 1 will be described with reference to FIGS. 2 to 4 are diagrams schematically showing a longitudinal sectional view of the inspection chip according to the embodiment.

  The inspection chip 1A of FIG. 1 according to the present embodiment includes a container part 13 in which a concave part 13a having a concave shape is formed, and a flat lid part 11. The container part 13 and the lid part 11 are liquid-tightly joined with a double-sided adhesive tape 17. A space surrounded by the container part 13 and the lid part 11 constitutes a reaction chamber 15. The lid component 11 is a biaxially stretched resin plate whose entire surface is hydrophilized with a surfactant. That is, the lid part 11 has both hydrophilicity and surface flatness. Therefore, the upper surface (ceiling surface) of the reaction chamber 15 is subjected to a hydrophilic treatment.

  The inspection chip 1B of FIG. 2 according to the present embodiment has a flat frame part 5 having an opening 3 through which a concave part 13a having a concave shape passes, and one end side of the opening 3 of the frame part 5. The flat bottom part 7 to be closed is liquid-tightly joined with a double-sided adhesive tape 17. The other end side of the opening 3 of the frame part 5 is closed by the lid part 11 via a double-sided adhesive tape 17.

  The bottom part 7 and the lid part 11 are biaxially stretched resin plates whose entire surface is hydrophilized with a surfactant. That is, it has both hydrophilicity and surface flatness. Therefore, the upper and lower surfaces (the ceiling surface and the bottom surface) of the reaction chamber 15 are subjected to a hydrophilic treatment.

  Further, the inspection chip 1C of FIG. 4 according to the present embodiment closes the double-sided pressure-sensitive adhesive tape 17 having the opening 3 through which the concave portion 13a having a concave shape passes, and one end side of the opening 3 of the double-sided pressure-sensitive adhesive tape 17. The flat bottom part 7 is liquid-tightly joined. The other end side of the opening 3 of the double-sided pressure-sensitive adhesive tape 17 is closed by the lid component 11. The lid part 11 and the bottom part 7 are biaxially stretched resin plates as in the above-described inspection chips 1A and 1B, and their surfaces are hydrophilized with a surfactant. That is, the upper and lower surfaces (ceiling surface and bottom surface) of the reaction chamber 15 are subjected to a hydrophilic treatment.

  Next, stabilization of fluidity in the inspection chip 1 will be described in the following examples.

  The inspection chip of Example 1 has the structure shown in FIGS. 1 and 4, and is obtained by bonding the bottom plate component 7 and the lid component 11 with the double-sided adhesive tape 17. The bottom plate component 7 and the lid component 11 are biaxially stretched PET (polyethylene terephthalate) resin plates whose surface is hydrophilized with a surfactant (trade name: Cosmo Shine A8300, manufactured by Toyobo Co., Ltd.). It was. As the double-sided pressure-sensitive adhesive tape, a double-sided pressure-sensitive adhesive tape (trade name: MC2030, manufactured by Nitto Denko Corporation) having a PET resin substrate was used. A space surrounded by these parts is a flow path (reaction chamber 15). Here, this is referred to as an inspection chip D. In addition, the dimension of a flow path is width 2mm and height 0.1mm.

  The bottom plate component 7 is a biaxially stretched PET (polyethylene terephthalate) resin plate, the surface of which is hydrophilized with a surfactant, while the lid component 11 is a normal one that has not been hydrophilized. An inspection chip E was manufactured in the same manner as in Example 1 except that a PET resin plate was used.

(Conventional example 1)
A conventional test chip F was manufactured in the same manner as in Example 1 except that a PVA-coated PET resin plate was used as the bottom plate component 7 and the lid component 11.

(Conventional example 2)
A conventional inspection chip G was manufactured in the same manner as in Example 1 described above except that a normal PET resin plate not subjected to the surfactant treatment was used as the bottom plate component 7 and the lid component 11.

Next, the fluidity of each inspection chip D, E, F, G manufactured as described above was evaluated.
As an evaluation method, pure water was dropped from the inlet 11a of the inspection target liquid, and the distance that entered the flow path was measured. The same test was performed 20 times, and the average value and standard deviation of the approach distance were calculated. The results are shown in Table 1.

  As can be seen from Table 1, the inspection chips D and E of the present invention have a longer distance of pure water and less variation than the inspection chips F and G according to the conventional example. . From this result, it can be said that the inspection chips D and E of the present invention have stable fluidity.

  Note that the present invention is not limited to the above-described embodiment, and can be modified as follows.

  (1) Since the effect of the present invention can be obtained by combining a biaxially stretched resin plate and a hydrophilic treatment capable of maintaining smoothness, the invention is not limited to the above-described embodiment.

  (2) The hydrophilic treatment may be performed not only on the ceiling surface and bottom surface or bottom surface of the reaction chamber 15 but also on the side surface of the reaction chamber 15. Alternatively, the hydrophilic treatment may be performed only on the side surface of the reaction chamber 15 without performing the hydrophilic treatment on the ceiling surface and the bottom surface. Since the hydrophilic treatment only needs to be performed on the surface constituting the reaction chamber 15, for example, the entire surface of the lid part 11 is not subjected to the hydrophilic treatment and only the portion corresponding to the ceiling surface of the reaction chamber 15 is hydrophilized. Processing may be performed. Thereby, the usage-amount of surfactant can be decreased.

  (3) The configuration of the inspection chip 1 is not limited to the above-described one, but may include the above-described equivalent inspection chip 1 by another structure.

The schematic structure of the chip | tip for a test | inspection which concerns on an Example is shown, (a) is a disassembled perspective view, (b) is a perspective view of the assembled state. It is the figure which showed typically the longitudinal cross-sectional view of the chip | tip for a test | inspection which concerns on an Example. It is the figure which showed typically the longitudinal cross-sectional view of the chip | tip for a test | inspection which concerns on an Example. It is the figure which showed typically the longitudinal cross-sectional view of the chip | tip for a test | inspection which concerns on an Example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1A-1C, D, E ... Inspection chip F, G ... Inspection chip concerning conventional example 3 ... Opening part 5 ... Frame body part 7 ... Bottom part 9 ... Opening 11 ... Lid part 11a ... Input port of liquid to be inspected 11b: Reagent inlet 11c: Measuring unit 13: Container part 13a: Recess 15: Reaction chamber 17 ... Double-sided adhesive tape

Claims (5)

A test chip for performing component analysis of a sample liquid as a specimen through a reaction chamber,
An inspection chip comprising at least one of the wall surfaces forming the reaction chamber having hydrophilicity and a biaxially stretched resin plate. The inspection chip according to claim 1,
The reaction chamber is a space formed by laminating a container part having a recessed structure and a flat lid part,
An inspection chip, wherein a hydrophilic treatment is applied to the container part and / or the lid part constituting the upper and lower surfaces of the reaction chamber. The inspection chip according to claim 2,
The inspection chip, wherein the container part includes a flat frame part having a penetrating opening and a flat bottom part that closes one end of the opening. The inspection chip according to claim 1,
The reaction chamber is a space formed by a flat lid component, a double-sided adhesive member having a penetrating opening, and a flat bottom component that closes one end of the opening,
An inspection chip, wherein a hydrophilic treatment is applied to the lid part and / or the bottom part constituting the upper and lower surfaces of the reaction chamber. The inspection chip according to any one of claims 1 to 4,
An inspection chip, wherein a hydrophilic treatment is applied to a part constituting the side surface of the reaction chamber.

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