JP2005087029A5 - - Google Patents

Claims (31)

  A culture apparatus for culturing a tissue piece, wherein the chamber forms a culture cavity that encloses the tissue piece so that the tissue piece can be cultured, and is opened and closed for carrying the tissue piece into and out of the culture cavity. A cavity opening / closing part, and a tissue piece clamping part for clamping the tissue piece at a predetermined position in the culture cavity, wherein at least one of the tissue piece clamping parts forms a culture surface, and the other contains the tissue piece. A culture device that presses and holds the cells so that they are in close contact with the culture surface.   The culture apparatus according to claim 1, wherein the tissue piece clamping unit releases and starts a state in which the tissue piece is clamped by the tissue piece clamping unit when the cavity opening / closing unit is opened and closed.   The culture apparatus according to claim 1 or 2, wherein the culture surface of the tissue piece sandwiching portion constitutes a part of the chamber.   The culture piece according to any one of claims 1 to 3, wherein the tissue piece sandwiching section includes a first sheet-like body and a second sheet-like body, and sandwiches the tissue piece between the sheet-like bodies. apparatus.   Of the peripheral edge portions of the first sheet-like body and the second sheet-like body that face each other, at least a part thereof is held together, and at least a part of the peripheral edge portion of the remaining part is openable to each other. The culture apparatus according to claim 4, wherein the culture apparatus is formed.   The culture apparatus according to claim 5, wherein the tissue piece sandwiching portion including the first sheet-like body and the second sheet-like body is formed in a bag-like body having a tissue piece insertion / extraction portion as an opening. .   The culture apparatus according to claim 5 or 6, wherein the tissue piece insertion / extraction part formed in the tissue piece clamping part is fixed so as to be positioned at or near the culture cavity opening / closing part.   The culture apparatus according to any one of claims 5 to 7, wherein the tissue piece taking in / out part opens and closes with the opening and closing of the culture cavity opening and closing part.   The culture apparatus according to claim 8, wherein the tissue piece insertion / extraction part constitutes at least a part of the culture cavity opening / closing part.   The culture device according to any one of claims 1 to 9, wherein a plurality of communication holes are provided in at least a part of the tissue piece sandwiching portion.   The culture device according to any one of claims 1 to 10, wherein at least a part of the tissue piece sandwiching portion is a mesh-like body.   The culture apparatus according to any one of claims 1 to 11, wherein a surface of the tissue piece sandwiching portion that faces the culture surface includes a dot-shaped or line-shaped convex portion.   The culture apparatus according to claim 1, wherein the culture cavity is formed so as to be able to be sealed at the cavity opening / closing part.   The culture apparatus according to claim 1, wherein at least a part of the chamber has flexibility.   The culture apparatus according to claim 14, wherein the chamber is a flexible bag-like body.   The culture apparatus according to claim 14 or 15, wherein the tissue piece sandwiching portion has a rigidity that does not follow the deformation of the chamber.   The culture apparatus according to claim 14 or 15, wherein the tissue piece sandwiching portion has flexibility.   The culture device according to any one of claims 1 to 17, wherein the tissue piece sandwiching part is formed so as to float in a certain range in the culture cavity during culture.   The culture apparatus according to claim 1, wherein the chamber is provided with a liquid take-in / out part.   The culture apparatus according to any one of claims 1 to 19, wherein the chamber includes a fluid injection / discharge section having a sealing material that can be punctured and maintain a sealing property.   The culture apparatus according to claim 1, wherein the chamber includes a gas discharge unit.   The culture apparatus according to claim 1, wherein the chamber has oxygen and carbon dioxide permeability.   The culture apparatus according to any one of claims 1 to 22, wherein the culture apparatus is a periosteum tissue piece culture apparatus. The culture apparatus according to any one of claims 1 to 23, wherein the tissue piece clamping unit is configured separately from the chamber.   A culture apparatus for culturing a tissue piece, comprising a chamber composed of a lid and a tray forming a culture cavity containing the tissue piece so that the tissue piece can be cultured, and provided in the chamber, A cavity opening / closing portion that opens and closes for loading and unloading; and a tissue piece clamping portion that clamps the tissue piece at a predetermined position in the culture cavity, wherein at least one of the tissue piece clamping portions forms a culture surface, A culture apparatus in which the other presses and holds the tissue piece in close contact with the culture surface. 26. The culture apparatus according to claim 25 , wherein the tissue piece sandwiching section is configured such that the culture surface is configured by the bottom surface of the tray and the other is provided on the lid. 27. The culture apparatus according to claim 25 or claim 26 , wherein the culture cavity is formed so as to be capable of being sealed at the cavity opening / closing portion, and the chamber is maintained in a state in which the sealing property of the culture cavity is maintained. A culture apparatus provided with a fluid pouring / discharging part capable of pouring / discharging fluid.   A tissue piece sandwiching part usable in the culture apparatus according to claim 24. A culture kit for culturing a tissue piece,
A chamber forming a culture cavity in which the tissue piece can be cultivated;
A tissue piece sandwiching portion that can clamp the tissue piece by pressing at least one of the culture surfaces and pressing the tissue piece in close contact with the culture surface, and can be included in a predetermined position in the culture cavity When,
A culture kit comprising:   30. The culture kit according to claim 29, wherein the chamber includes a fluid pouring / discharging unit capable of pouring / discharging the fluid in the chamber while maintaining the sealing property of the culture cavity.   The culture kit according to claim 30, further comprising a syringe capable of pouring and discharging a fluid into the culture cavity via the fluid pouring and discharging unit.