JP2005046049A - Novel fermented soy milk and method for producing the same - Google Patents

Novel fermented soy milk and method for producing the same Download PDF

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JP2005046049A
JP2005046049A JP2003281384A JP2003281384A JP2005046049A JP 2005046049 A JP2005046049 A JP 2005046049A JP 2003281384 A JP2003281384 A JP 2003281384A JP 2003281384 A JP2003281384 A JP 2003281384A JP 2005046049 A JP2005046049 A JP 2005046049A
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soybean
soybeans
koji
soy milk
soymilk
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Takao Ota
隆男 太田
Tsuyoshi Miyakoshi
強 宮腰
Fumiko Miura
史子 三浦
Sakiko So
咲子 荘
Akihiko Sukeno
彰彦 助野
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HISHIROKU KK
NIPPON SHOKUHIN KAIHATSU KENKY
NIPPON SHOKUHIN KAIHATSU KENKYUSHO KK
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HISHIROKU KK
NIPPON SHOKUHIN KAIHATSU KENKY
NIPPON SHOKUHIN KAIHATSU KENKYUSHO KK
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a novel soybean product comprising fermented soy milk improved in palatability and excellent in functionality; and to provide a method for producing the novel soybean product such as the novel fermented soy milk. <P>SOLUTION: The method for producing fermented soy milk comprises making soy bean, malt and water exist together. The fermented soy milk is obtained by the method. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

本発明は、新規の発酵豆乳およびその製造方法に関する。   The present invention relates to a novel fermented soymilk and a method for producing the same.

従来、発酵豆乳の製造方法は主に乳酸菌を豆乳に添加して乳酸発酵させるものであり、製造されるものは、乳酸による酸味を有する、いわゆる乳酸飲料である(例えば特許文献1)。該従来の発酵豆乳は、その強い酸味故、豆乳や調製豆乳と全く嗜好が異なり、また食材としての用途範囲が限定される。   Conventionally, fermented soymilk is produced mainly by adding lactic acid bacteria to soymilk to cause lactic acid fermentation, and what is produced is a so-called lactic acid beverage having a sour taste from lactic acid (for example, Patent Document 1). The conventional fermented soymilk has a completely different taste from soymilk and prepared soymilk because of its strong acidity, and its range of use as a foodstuff is limited.

また、機能性の付加(γ−アミノ酪酸の増加:特許文献2)や大豆アレルゲンの低減(特許文献3)を目的として豆乳に麹を添加したものが知られているが、それらは風味や嗜好性について通常の豆乳、調製豆乳と何ら変わらなかったとしている。   Moreover, what added koji to soybeans for the purpose of functional addition (increase in γ-aminobutyric acid: Patent Document 2) and reduction of soybean allergen (Patent Document 3) is known. It is said that there was no difference in sex from ordinary soymilk and prepared soymilk.

一方、豆乳、調製豆乳については、大豆臭の存在がその嗜好性を悪くしているが、この大豆臭は原料の大豆に含まれるリノール酸等の不飽和脂肪酸が、同じく大豆に含まれる酸化酵素であるリポキシゲナーゼの作用により過酸化脂質を生成し、次いで脱離酵素であるハイドロペルオキシドリアーゼの作用により大豆臭の主成分であるn−ヘキサナールを生成するためである。豆乳、調製豆乳の大豆臭を生じないようにする方法として、大豆を80℃以上の高温水と共に磨砕してリポキシゲナーゼを失活させる方法(特許文献4)や、リポキシゲナーゼを完全に欠損させた大豆(農林103号、九州111号)を用いる方法等が知られている。   On the other hand, for soy milk and prepared soy milk, the presence of soy odor makes the palatability worse, but this soy odor is an unsaturated fatty acid such as linoleic acid contained in the raw soybean, which is also an oxidase enzyme contained in soybean. This is because lipid peroxide is produced by the action of lipoxygenase, and n-hexanal, which is the main component of soybean odor, is produced by the action of hydroperoxide lyase, which is a desorption enzyme. As a method for preventing soy odor from being produced in soy milk and prepared soy milk, a method of inactivating lipoxygenase by grinding soybean with high-temperature water at 80 ° C. or higher (Patent Document 4), or a soybean in which lipoxygenase is completely deficient A method using (Agriculture 103, Kyushu 111) is known.

また、豆乳には大豆臭だけでなく、サポニンや大豆蛋白由来の苦味、ゲニスチンやダイジンといったイソフラボノイド由来の収斂味等いわゆる不快味がある。これを改善するため、豆乳に酵素処理を施し、後味が改善したことが報告されている。   In addition to soy odor, soy milk has a so-called unpleasant taste such as a bitter taste derived from saponin and soybean protein, and an astringent taste derived from isoflavonoids such as genistin and daidin. In order to improve this, it has been reported that the soymilk is treated with an enzyme to improve the aftertaste.

しかし、上記の豆乳の改良は、いずれも従来大豆や豆乳の微細な改良にすぎず、従来の豆乳から大幅な味の改善までは至っていない。   However, any of the above-mentioned improvements in soymilk is only a fine improvement of conventional soybeans and soymilk, and has not led to a significant improvement in taste from conventional soymilk.

大豆食品は、栄養学的に優れており、また機能性についても近年様々な報告がされて注目を集めている。特に、こうした大豆食品の持つ有効成分を効率よく摂取することができる機能性食品として豆乳が注目されている。しかし、上記のように従来の豆乳は、原料の大豆に起因する大豆臭、苦味や収斂性等の不快味が大きな問題となっている。これを改善するため、乳酸菌を豆乳に添加して発酵させる方法や、豆乳を酵素剤で処理する方法が報告されているが、乳酸菌発酵した豆乳は、酸味が強く、酵素剤で処理した豆乳は、蛋白分解酵素により疎水性のアミノ酸が増え、苦味が出る。
したがって、万人に好まれる嗜好性の高い新規の大豆製品の開発が望まれている。
特開平11−46685号公報 特開平11−103825号公報 特開平7−203890号公報 特開平11−46685号公報
Soy foods are nutritionally superior and have recently attracted attention with various reports on functionality. In particular, soy milk has attracted attention as a functional food that can efficiently ingest the active ingredients of such soybean foods. However, as described above, the conventional soymilk has significant problems of unpleasant taste such as soybean odor, bitterness and astringency due to the raw material soybean. In order to improve this, methods of fermenting by adding lactic acid bacteria to soy milk and methods of processing soy milk with an enzyme agent have been reported, but soy milk fermented with lactic acid bacteria has a strong acidity, soy milk treated with an enzyme agent is , Proteolytic enzymes increase hydrophobic amino acids, resulting in a bitter taste.
Therefore, development of a new soy product with high palatability preferred by everyone is desired.
Japanese Patent Laid-Open No. 11-46685 JP-A-11-103825 JP-A-7-203890 Japanese Patent Laid-Open No. 11-46685

本発明は、新規の大豆製品の提供、および該新規の大豆製品の製造方法の提供を目的とする。   An object of the present invention is to provide a novel soybean product and a method for producing the novel soybean product.

本発明者らは、上記目的を達成するために鋭意研究を重ねた結果、大豆、麹および水を共存させて発酵させることで、嗜好性が向上し、かつ機能性に優れた新規発酵豆乳が得られることを見出して、本発明を完成させるに至った。即ち、本発明の新規発酵豆乳は、豆乳本来の美味しさが保たれ、麹に由来する適度な甘みや香気、および濃厚さが付加され、麹菌の産出する種々の酵素が適度に作用することにより大豆臭が減少し、麹に由来する糖などによりマスキングされて苦味や収斂性等の不快味が緩和される等した嗜好性の向上した発酵豆乳であり、かつ、麹菌の産出する種々の酵素が適度に作用することにより、栄養価および消化吸収が向上した機能性に優れた発酵豆乳である。したがって、本発明の新規発酵豆乳は、食材として用途範囲の広いものである。   As a result of intensive studies to achieve the above object, the present inventors have made fermented soymilk with improved palatability and functionality by fermenting soybeans, straw and water together. As a result, the present invention has been completed. That is, the novel fermented soymilk of the present invention maintains the original taste of soymilk, is added with moderate sweetness and aroma derived from koji, and rich, and various enzymes produced by koji molds act appropriately. It is fermented soymilk with reduced soy odor, masked by sugar derived from koji, and alleviating unpleasant tastes such as bitterness and astringency, etc., and various enzymes produced by koji molds It is a fermented soymilk with excellent functionality with improved nutritional value and digestion and absorption by acting moderately. Therefore, the novel fermented soymilk of the present invention has a wide range of uses as a food material.

すなわち、本発明は以下の通りである。
[1]大豆、麹および水を共存させる工程を含む発酵豆乳の製造方法。
[2]水を加えながら大豆を磨砕して懸濁液を得て、得られた懸濁液に麹を添加する工程を含む上記[1]記載の製造方法。
[3]さらに、ろ過する工程を含む、上記[1]または[2]に記載の製造方法。
[4]麹に由来する酵素が作用し得る条件下で行う、上記[1]〜[3]のいずれかに記載の製造方法。
[5]麹に由来する酵素が、糖質関連酵素または蛋白質分解酵素である、上記[4]記載の製造方法。
[6]大豆が、乾燥大豆、水煮大豆、大豆粉末、または脱脂大豆から選ばれる大豆である、上記[1]〜[5]のいずれかに記載の製造方法。
[7]上記[1]〜[6]のいずれかの製造方法により製造される発酵豆乳。
That is, the present invention is as follows.
[1] A method for producing fermented soymilk comprising a step of allowing soybeans, straw and water to coexist.
[2] The production method of the above-mentioned [1], comprising a step of grinding soybeans while adding water to obtain a suspension, and adding koji to the obtained suspension.
[3] The production method according to [1] or [2], further including a filtering step.
[4] The production method according to any one of the above [1] to [3], which is performed under conditions under which an enzyme derived from salmon can act.
[5] The production method of the above-mentioned [4], wherein the enzyme derived from koji is a sugar-related enzyme or a proteolytic enzyme.
[6] The production method according to any one of [1] to [5], wherein the soybean is a soybean selected from dried soybeans, boiled soybeans, soybean powder, or defatted soybeans.
[7] Fermented soymilk produced by the production method of any one of [1] to [6] above.

本発明によれば、麹に由来する適度な甘味や香気、濃厚さの付加により嗜好性を向上し、更に麹菌の産出する種々酵素が適度に作用することにより、栄養価および消化吸収の向上、大豆臭の減少等を生じ、また、麹に由来する糖などによりマスキングされて苦味や収斂性等の不快味が緩和された、万人に美味しい、食材としての用途範囲の広い今までにない新規発酵豆乳を得ることができる。   According to the present invention, moderate sweetness and aroma derived from persimmon, improving the palatability by adding a thick, further various nutrients produced by koji molds, the nutritional value and digestion absorption, Reduced soy odor, etc., masked by sugar derived from koji, eased unpleasant tastes such as bitterness and astringency, etc. Fermented soymilk can be obtained.

本発明にいう麹とは、麹菌を穀物(例えば米、麦、豆等)等の食品素材に接種して生育させたものをいう。麹菌は本発明に有効な酵素を生成するものであって、食して害のない菌であれば特に制限はなく、例えば、アスペルギルス(Aspergillus)属、ペニシリウム(Penicillium)属、ムコール(Mucor)属、リゾーブス(Rhizopus)属、モナスカス(Monascus)属、または、アブシディア(Absidia)属に属する微生物が挙げられ、具体例としては、例えば、アスペルギルス(Aspergillus)属のオリゼー(oryzae)等が挙げられる。   The koji used in the present invention refers to a koji mold inoculated and grown on a food material such as cereal grains (eg, rice, wheat, beans). Aspergillus is one that produces an enzyme that is effective for the present invention, and is not particularly limited as long as it is a harmful organism that eats. For example, Aspergillus genus, Penicillium genus, Mucor genus, Examples include microorganisms belonging to the genus Rhizopus, Monascus, or Absidia, and specific examples include, for example, Oryzae of the genus Aspergillus.

麹菌は接種する相手(穀物等の食品素材)の種類によって米麹、麦麹、豆麹等となる。本発明ではこれらのいずれを用いてもよいが米麹が好ましい。米麹とは蒸米に麹菌を繁殖させたものであり、例えば商品名:菱六米こうじ、株式会社菱六製等が挙げられる。   Neisseria gonorrhoeae can be rice bran, wheat straw, bean straw, etc., depending on the type of inoculation partner (food material such as grain). In the present invention, any of these may be used, but rice bran is preferred. Rice bran is a product obtained by breeding koji mold on steamed rice, and examples thereof include trade names: Rokurokumai Koji, Ryokuroku Co., Ltd., and the like.

麹菌はその種類によって多種多様な酵素を生成し、また、同じ麹菌であってもその生育過程の違い、例えば品温経過の違い等によって生成する酵素のバランスが異なるが、本発明に有効な酵素を生成するものであればこれらのいずれを用いてもよい。   Aspergillus oryzae produce a wide variety of enzymes depending on the type of the koji mold, and even if the koji mold is the same, the balance of the enzymes produced varies depending on the growth process, for example, the difference in the temperature of the product. Any of these may be used as long as they generate.

麹に由来する酵素としては、特に限定されず、例えば糖質関連酵素、蛋白質分解酵素等が挙げられる。糖質関連酵素とは、澱粉、繊維素、ヘミセルロース、ペクチン等の多糖類及びそれらを構成する単糖、オリゴ糖に作用する酵素をいい、例えば、α−アミラーゼ、α−グルコシダーゼ、グルコアミラーゼ、イソアミラーゼ、トランスフェラーゼ、β−グルカナーゼ、β−グルコシダーゼ、キシラナーゼ、ガラクトマンナナーゼ、ペクチナーゼ、ペクチンエステラーゼ等が挙げられる。
蛋白質分解酵素としては、例えば酸性プロテアーゼ、中性プロテアーゼ、酸性カルボキシペプチダーゼ、アミノペプチダーゼ等が挙げられる。
The enzyme derived from koji is not particularly limited, and examples thereof include carbohydrate-related enzymes and proteolytic enzymes. Sugar-related enzymes refer to enzymes that act on polysaccharides such as starch, fibrin, hemicellulose, pectin and the like, and monosaccharides and oligosaccharides constituting them, such as α-amylase, α-glucosidase, glucoamylase, Examples include amylase, transferase, β-glucanase, β-glucosidase, xylanase, galactomannanase, pectinase, and pectinesterase.
Examples of proteolytic enzymes include acid protease, neutral protease, acid carboxypeptidase, aminopeptidase and the like.

麹は、公知方法により上記麹菌を任意の原料(穀物等の食品素材)に接種し生育させて得ることができるが、市販されているもの(例えば商品名:菱六米こうじ、株式会社菱六製等)を用いてもよい。   The koji can be obtained by inoculating and growing any of the above koji molds (food materials such as cereals) using known methods, but commercially available ones (for example, trade names: Rokurokumai Koji, Ryokuroku Co., Ltd.) May be used.

(1)大豆、麹および水を共存させる工程
本発明は、大豆、麹および水を共存させて発酵させることを特徴とする。具体的には、例えば、上記麹菌を任意の原料(穀物等の食品素材)に接種することで得られた麹を磨砕した大豆に添加してその混合物を含水状態に維持し、雑菌の繁殖を抑える温度帯で任意の時間処理して発酵させることが挙げられる。
(1) Process in which soybean, straw and water coexist The present invention is characterized in that soybean, straw and water coexist and fermented. Specifically, for example, the koji obtained by inoculating the koji mold on any raw material (food material such as cereal) is added to the ground soybean, and the mixture is maintained in a water-containing state, so that the propagation of bacteria It is possible to ferment by treating for an arbitrary time in a temperature range that suppresses the temperature.

大豆、麹および水を共存させるための添加の順序等は特に限定されない。大豆、麹および水を共存させるには、例えば、水を加えながら大豆を磨砕して懸濁液を得て、得られた懸濁液に麹を添加する方法、また、大豆粉末に水を加えた後、麹を添加する方法等が挙げられる。   There is no particular limitation on the order of addition for allowing soybean, straw and water to coexist. In order to allow soybeans, straw and water to coexist, for example, the soybean is ground by adding water to obtain a suspension, and then the straw is added to the obtained suspension. After adding, the method of adding soot, etc. are mentioned.

原料である大豆は、加工等したものであってもよく、例えば乾燥大豆、水煮大豆、大豆粉末、脱脂大豆等のいずれも用いることができる。例えば脱脂した大豆粉等であってもよい。
ここに、乾燥大豆とは丸大豆を乾燥させた通常流通しているものをいう。水煮大豆とは丸大豆を水に浸漬後、蒸煮して組織を軟化させたものをいう。大豆粉末とは丸大豆を粉砕したものをいう。脱脂大豆とは、一般に脱脂大豆と言われる丸大豆の油を工業的に取った残りで油分約0.9%であるものの他、低脂大豆(油分約5〜6%)、高脂大豆(油分約15%)等も含む概念である。
大豆は、磨砕したものを用いることが好ましい。
The raw material soybeans may be processed ones such as dried soybeans, boiled soybeans, soybean powder, and defatted soybeans. For example, defatted soybean powder may be used.
Here, the dry soybean means a normally circulated dried soybean. Boiled soybeans are those obtained by soaking whole soybeans in water and then boiled to soften the tissue. Soybean powder is pulverized whole soybeans. The defatted soybean is a residue of about 0.9% oil that is obtained by industrially removing the oil of whole soybean, which is generally called defatted soybean, as well as low-fat soybean (oil content about 5-6%), high-fat soybean ( This is a concept including about 15% of oil).
It is preferable to use soybeans that have been ground.

大豆の磨砕は、大豆加工食品の分野で自体公知の方法等により行えばよい。磨砕の程度は特に限定されない。   The grinding of soybeans may be performed by a method known per se in the field of processed soybean foods. The degree of grinding is not particularly limited.

乾燥大豆を用いる場合は、大豆の組織を軟化させるために、予め磨砕前に水に浸漬しておくことが好ましい。   In the case of using dried soybeans, it is preferable to immerse them in water before grinding in order to soften the soybean tissue.

麹の使用量は、大豆と水の総量(100重量部)に対して、通常5重量部〜50重量部、好ましくは10重量部〜30重量部である。
水の使用量は、使用する大豆の状態(乾燥、水煮等)等によっても異なるが、一般に、乾燥大豆100重量部に対して、通常500重量部〜2000重量部、好ましくは600重量部〜1000重量部である。
The amount of koji used is usually 5 to 50 parts by weight, preferably 10 to 30 parts by weight, based on the total amount of soybean and water (100 parts by weight).
The amount of water used varies depending on the state of soybean used (dried, boiled, etc.), but generally 500 parts by weight to 2000 parts by weight, preferably 600 parts by weight to 100 parts by weight of dried soybeans. 1000 parts by weight.

本工程は、使用する麹に由来する酵素が作用し得る条件下で行うことが好ましい。
例えば、通常5〜85℃、好ましくは40〜65℃の条件下で行うことが挙げられる。
本工程は、また、雑菌の繁殖を抑制する観点からは、他の微生物が繁殖しにくい温度帯、例えば60℃以上で行うことが好ましい。
また、麹の作用を受けやすくするために、麹を後で添加する場合において麹を添加する前に、大豆と水との混合物を80〜130℃で、10秒〜60分、加熱処理しておくことが好ましい。
This step is preferably performed under conditions where an enzyme derived from the koji used can act.
For example, it is usually performed under conditions of 5 to 85 ° C, preferably 40 to 65 ° C.
In addition, this step is preferably performed in a temperature range in which other microorganisms hardly propagate, for example, 60 ° C. or more, from the viewpoint of suppressing the propagation of various bacteria.
In addition, in order to make it easier to receive the action of the koji, when adding koji later, before adding koji, the mixture of soybean and water is heated at 80 to 130 ° C. for 10 seconds to 60 minutes. It is preferable to keep it.

本工程は、通常1〜72時間、好ましくは3〜7時間で行う。   This step is usually performed for 1 to 72 hours, preferably 3 to 7 hours.

(2)ろ過する工程
本発明では、上記の工程(1)の後、ろ過することが好ましい。ろ過は通常の方法により行うことができるが、例えば、局方ガーゼによるろ過、布袋によるろ過、連続遠心分離機(スクリューデカンター)による固液分離等が挙げられる。
(2) The process to filter In this invention, it is preferable to filter after said process (1). Filtration can be performed by an ordinary method, and examples thereof include filtration with pharmacopoeia gauze, filtration with a cloth bag, and solid-liquid separation with a continuous centrifuge (screw decanter).

このようにして得られた本発明の新規発酵豆乳は、そのまま発酵豆乳飲料としても良く、また、様々な食品(例えば製パン、焼菓子、たれ、ソース類等)の調味ベースとして用いることもできる。   The novel fermented soymilk of the present invention thus obtained may be used as it is as a fermented soymilk beverage, and can also be used as a seasoning base for various foods (for example, bread making, baked confectionery, sauce, sauces, etc.). .

以下、本発明を詳細に説明するために実施例を記載するが、本発明はこれらの実施例によって何ら限定されるものではない。   EXAMPLES Hereinafter, examples will be described to describe the present invention in detail, but the present invention is not limited to these examples.

大豆150gを600mLの水に一夜浸漬して、磨砕処理した後、得られた混合物に沸騰した等量の熱湯を加えて混合し、そのまま10分間弱火で煮た。その後60℃まで冷却し、米麹(蒸米に麹菌(株式会社菱六保存菌株)を繁殖させ、分生子が殆ど生じない時期を目標として仕上げたもの)を160g添加して60℃で5時間保持し、局方ガーゼでろ過して、本発明の発酵豆乳を得た。   After 150 g of soybeans were soaked in 600 mL of water overnight and ground, an equal amount of boiling water was added to the resulting mixture, mixed, and boiled for 10 minutes. Thereafter, the mixture was cooled to 60 ° C, and 160g of rice bran (branch fungus (Ryokuroku preservation strain) was propagated in steamed rice and finished with a target when almost no conidia were generated) was added and held at 60 ° C for 5 hours. And it filtered with pharmacopoeia gauze and obtained fermented soymilk of this invention.

Claims (7)

大豆、麹および水を共存させる工程を含む発酵豆乳の製造方法。   A method for producing fermented soymilk, comprising a step of allowing soybeans, straw and water to coexist. 水を加えながら大豆を磨砕して懸濁液を得て、得られた懸濁液に麹を添加する工程を含む請求項1記載の製造方法。   The production method according to claim 1, further comprising the step of grinding the soybean while adding water to obtain a suspension, and adding koji to the resulting suspension. さらに、ろ過する工程を含む、請求項1または2に記載の製造方法。   Furthermore, the manufacturing method of Claim 1 or 2 including the process to filter. 麹に由来する酵素が作用し得る条件下で行う、請求項1〜3のいずれかに記載の製造方法。   The manufacturing method in any one of Claims 1-3 performed on the conditions which the enzyme derived from a cocoon can act. 麹に由来する酵素が、糖質関連酵素または蛋白質分解酵素である、請求項4記載の製造方法。   The production method according to claim 4, wherein the enzyme derived from koji is a carbohydrate-related enzyme or a proteolytic enzyme. 大豆が、乾燥大豆、水煮大豆、大豆粉末、または脱脂大豆から選ばれる大豆である、請求項1〜5のいずれかに記載の製造方法。   The production method according to any one of claims 1 to 5, wherein the soybean is a soybean selected from dried soybeans, boiled soybeans, soybean powder, or defatted soybeans. 請求項1〜6のいずれかの製造方法により製造される発酵豆乳。   Fermented soymilk produced by the production method according to claim 1.
JP2003281384A 2003-07-28 2003-07-28 Novel fermented soy milk and method for producing the same Pending JP2005046049A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2007000980A1 (en) * 2005-06-27 2009-01-22 和弘 堤 Soy citrate beverage and method for producing the same
JP2021122266A (en) * 2020-01-31 2021-08-30 株式会社元気にやせる研究所 Method for producing fermented food, method for producing cookie-like fermented food, sleep inducing agent

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2007000980A1 (en) * 2005-06-27 2009-01-22 和弘 堤 Soy citrate beverage and method for producing the same
JP4592106B2 (en) * 2005-06-27 2010-12-01 和弘 堤 Soy citrate beverage and method for producing the same
JP2021122266A (en) * 2020-01-31 2021-08-30 株式会社元気にやせる研究所 Method for producing fermented food, method for producing cookie-like fermented food, sleep inducing agent

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