JP2005037303A5 - - Google Patents

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Publication number
JP2005037303A5
JP2005037303A5 JP2003276221A JP2003276221A JP2005037303A5 JP 2005037303 A5 JP2005037303 A5 JP 2005037303A5 JP 2003276221 A JP2003276221 A JP 2003276221A JP 2003276221 A JP2003276221 A JP 2003276221A JP 2005037303 A5 JP2005037303 A5 JP 2005037303A5
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JP
Japan
Prior art keywords
hollow
hollow fiber
capture probe
gel
fiber
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JP2003276221A
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Japanese (ja)
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JP2005037303A (en
JP4222903B2 (en
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Priority to JP2003276221A priority Critical patent/JP4222903B2/en
Priority claimed from JP2003276221A external-priority patent/JP4222903B2/en
Publication of JP2005037303A publication Critical patent/JP2005037303A/en
Publication of JP2005037303A5 publication Critical patent/JP2005037303A5/ja
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Publication of JP4222903B2 publication Critical patent/JP4222903B2/en
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Expired - Fee Related legal-status Critical Current

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Claims (3)

以下の（１）〜（４）の工程を順次行うことを含む生体関連物質検出用マイクロアレイの製造方法。
（１） 複数本の有機材料からなる中空繊維を、それらの中空繊維の各繊維軸が同一方向となるように配列し、その配列を固定して、中空繊維束を製造する工程。
（２） 中空繊維束を水で飽和する工程。
（３） キャプチャープローブを含むゲル前駆体溶液を各中空繊維の中空部に導入し、中空部内で重合反応を行い、キャプチャープローブを含むゲル状物を中空繊維の中空部に保持する工程。
（４） 中空繊維束を繊維の長手方向に交叉する方向で切断して薄片化する工程。 A method for producing a microarray for detecting biologically relevant substances, comprising sequentially performing the following steps (1) to (4).
(1) A step of manufacturing a hollow fiber bundle by arranging hollow fibers made of a plurality of organic materials so that the fiber axes of the hollow fibers are in the same direction and fixing the arrangement.
(2) A step of saturating the hollow fiber bundle with water.
(3) The process of introduce | transducing the gel precursor solution containing a capture probe into the hollow part of each hollow fiber, performing a polymerization reaction in a hollow part, and hold | maintaining the gel-like substance containing a capture probe in the hollow part of a hollow fiber.
(4) A step of cutting the hollow fiber bundle in a direction crossing the longitudinal direction of the fiber to make a thin piece. 以下の（１）〜（４）の工程を順次行うことを含む生体関連物質検出用マイクロアレイの製造方法。
（１） 有機材料からなる中空繊維を水で飽和する工程。
（２） キャプチャープローブを含むゲル前駆体溶液を前記中空繊維の中空部に導入し、中空部内で重合反応を行い、キャプチャープローブを含むゲル状物を中空繊維の中空部に保持する工程。
（３） 複数本の前記中空繊維をそれらの繊維軸が同一方向となるように配列し、その配列を固定して、中空繊維束を製造する工程。
（４） 中空繊維束を繊維の長手方向に交叉する方向で切断して薄片化する工程。 A method for producing a microarray for detecting biologically relevant substances, comprising sequentially performing the following steps (1) to (4).
(1) A step of saturating hollow fibers made of an organic material with water.
(2) The process of introduce | transducing the gel precursor solution containing a capture probe into the hollow part of the said hollow fiber, performing a polymerization reaction in a hollow part, and hold | maintaining the gel-like thing containing a capture probe in the hollow part of a hollow fiber.
(3) A step of producing a hollow fiber bundle by arranging a plurality of the hollow fibers so that their fiber axes are in the same direction and fixing the arrangement.
(4) A step of cutting the hollow fiber bundle in a direction crossing the longitudinal direction of the fiber to make a thin piece. 以下の（１）〜（４）の工程を順次行うことを含む生体関連物質検出用マイクロアレイの製造方法。
（１） 互いに平行に配置された複数の貫通孔を含む有機材料からなるブロックを製造する工程。
（２） ブロックを水で飽和する工程。
（３） キャプチャープローブを含むゲル前駆体溶液を各貫通孔に導入し、貫通孔内で重合反応を行い、キャプチャープローブを含むゲル状物を貫通孔に保持する工程。
（４） ブロックを貫通孔の長手方向と交叉する方向で切断して薄片化する工程。 A method for producing a microarray for detecting biologically relevant substances, comprising sequentially performing the following steps (1) to (4).
(1) A step of manufacturing a block made of an organic material including a plurality of through holes arranged in parallel to each other.
(2) A step of saturating the block with water.
(3) A step of introducing a gel precursor solution containing a capture probe into each through hole, performing a polymerization reaction in the through hole, and holding a gel-like material containing the capture probe in the through hole.
(4) A step of cutting the block in a direction crossing the longitudinal direction of the through hole to make a thin piece.
JP2003276221A 2003-07-17 2003-07-17 Method for producing microarray for detecting biologically relevant substances Expired - Fee Related JP4222903B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003276221A JP4222903B2 (en) 2003-07-17 2003-07-17 Method for producing microarray for detecting biologically relevant substances

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003276221A JP4222903B2 (en) 2003-07-17 2003-07-17 Method for producing microarray for detecting biologically relevant substances

Publications (3)

Publication Number Publication Date
JP2005037303A JP2005037303A (en) 2005-02-10
JP2005037303A5 true JP2005037303A5 (en) 2006-08-31
JP4222903B2 JP4222903B2 (en) 2009-02-12

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003276221A Expired - Fee Related JP4222903B2 (en) 2003-07-17 2003-07-17 Method for producing microarray for detecting biologically relevant substances

Country Status (1)

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JP (1) JP4222903B2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006292367A (en) * 2005-04-05 2006-10-26 Mitsubishi Rayon Co Ltd Microarray for detecting mirna
JP5660468B2 (en) * 2011-03-15 2015-01-28 三菱レイヨン株式会社 Method for producing gel microarray for detecting bio-related substances

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