JP2004526952A5 - - Google Patents

Claims (13)

An in-process specific mass spectrometry method comprising:
A step you prepare a sample having an analyte,
A step of preparing isotopically enriched spike regarding the analyte,
In addition spike the sample, the steps of them to equilibrium,
A process of creating ions from an equilibrium spike and sample by atmospheric pressure ionization,
Introducing ions into the mass spectrometer so that the mass spectrometer exhibits an analyte response and a spike response;
Using a ratio of the analyte response to the spike response in the processor to determine the amount of analyte in the sample . The in-process specific mass spectrometry method according to claim 1, wherein the step of preparing the sample is a step of preparing a liquid sample. 3. The in-process specific mass spectrometry method according to claim 2, wherein the step of preparing the liquid sample is a step of preparing an aqueous sample. The in-process specific mass spectrometry method according to claim 1, wherein the step of preparing a sample is a step of preparing a sample having an analyte as one or a plurality of impurities. An in-process specific mass spectrometer,
An apparatus for receiving a sample configured to receive a sample having an analyte ;
A spike introduction device for introducing at least one isotope enriched spike into the sample and equilibrating them ;
An atmospheric pressure ion generator that receives the equilibrated sample and spike and ionizes them ;
A mass spectrometer that receives and processes the ions to show an analyte response and a spike response;
An apparatus comprising: a processor that determines the amount of analyte in the sample using a ratio of the analyte response to the spike response . 6. The in-process specific mass spectrometer of claim 5 , further comprising a sample analyzer that analyzes the sample and delivers analysis information of the sample to the processor. 7. The in-process specific mass spectrometer of claim 6 , further comprising a controller that receives information processed by the processor and feeds it back to other parts of the apparatus. 6. The in-process specific mass spectrometer of claim 5 , further comprising a sample modifier that changes the properties of the sample in response to a signal from the controller before the sample enters the atmospheric pressure ionizer. 9. The in-process specific mass spectrometer of claim 8 , wherein the controller coordinates the operation of the sample receiving device, the spike introduction device, the sample modification device, the atmospheric pressure ion generator, and the mass spectrometer. A solution processing apparatus disposed between the sample reforming apparatus and the atmospheric pressure ion generator;
The solution treatment unit are cooperative to, before sending the atmospheric pressure ion generator, the equilibrium in which are least further comprise one chromatographic advance concentrated sample and spike, according to claim 9 in processes Specific mass spectrometer. The in-process specific mass spectrometer according to claim 10 , wherein the chromatograph is selected from the group consisting of a liquid chromatograph and a gas chromatograph. Apparatus for receiving the sample has a first outlet conduit communicating with the spike introduction device having an outlet conduit communicating with the chemical reformer, and a second conduit communicating with a chemical reformer claim 10 in -process specific mass spectrometers. 6. The in-process specific mass spectrometer of claim 5 , wherein the atmospheric pressure ion generator is an electrospray ionizer.