KR101068996B1 - A method for verification of animal age based on stable isotopic ratio measurement - Google Patents
A method for verification of animal age based on stable isotopic ratio measurement Download PDFInfo
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Abstract
본 발명은 생물학적 반감기를 이용하여 동물의 출생시 관심 원소 중 특정 동위원소를 농축시켜 투여한 이후, 도살 또는 유통과정에서 생체조직의 표본을 취하여 표본 내 관심 원소의 동위원소비를 측정함으로써 도살 시점에서의 동물의 월령을 측정하는 방법에 대한 것이다.According to the present invention, a biological half-life is used to concentrate a specific isotope of an element of interest at the time of birth of an animal, and then, after slaughtering or distribution, take a sample of biological tissue and measure the isotope ratio of the element of interest in the sample. Is about how to measure the age of an animal.
본 발명의 월령 측정 방법에 의해 종래의 치아감별법의 부정확성을 보완할 수 있으며, 출생시 일정량의 동위원소 화합물의 투여를 통해 유통 단계에서 동물의 월령을 확인할 수 있어 고령 쇠고기의 시장 유통을 관리할 수 있으며, 광우병 우려로 인한 불안을 해소하는데 이바지할 수 있다. 또한 다양한 동물에서 동위원소의 반감기를 측정함으로써 소 이외의 기타 포유 동물의 나이 측정에도 응용될 수 있다. The age measurement method of the present invention can compensate for the inaccuracies of the conventional dental differentiation method, and it is possible to check the age of the animal at the distribution stage by administering a certain amount of isotope compound at birth, thereby managing the market distribution of the aged beef. It can also help to relieve anxiety caused by mad cow disease. It can also be applied to the determination of the age of mammals other than cattle by measuring the half-life of isotopes in various animals.
월령 측정, 동위원소, 생물학적 반감기 Age measurement, isotope, biological half-life
Description
본 발명은 생물학적 반감기를 이용하여 동물의 출생시 관심 원소 중 특정 동위원소를 농축시켜 투여한 이후, 도살 또는 유통과정에서 생체조직의 표본을 취하여 표본 내 관심 원소의 동위원소비를 측정함으로써 도살 시점에서의 동물의 월령을 측정하는 방법에 대한 것이다.According to the present invention, a biological half-life is used to concentrate a specific isotope of an element of interest at the time of birth of an animal, and then, after slaughtering or distribution, take a sample of biological tissue and measure the isotope ratio of the element of interest in the sample. Is about how to measure the age of an animal.
최근 광우병 파동 및 미국산 소고기의 수입과 관련하여 수입된 소고기가 30개월 미만의 소의 것인지에 대한 측정 방법이 문제되고 있다.Recently, in relation to the mad cow disease wave and the import of US beef, there is a problem of measuring whether the imported beef is from cattle less than 30 months old.
이와 관련하여 동물의 치아를 조사하여 나이를 감별하는 치아감별법을 통해 동물의 월령을 측정할 수 있다는 의견이 있지만, 소의 치아(dentition)는 품종, 지역적 위치, 유전적 특성, 먹이 종류, 사육 환경, 영양 상태 등에 따라 다양한 개체차이 존재하기 때문에 통계적으로 상대적 나이를 추정하는 자료로 이용할 수 있으나 절대적 나이를 판정하는 지표로 사용할 수 없다는 것이 과학적 입장이다. 그리고 유통중의 육류에 대하여는 적용할 수 없는 단점도 있다. In this regard, there is an opinion that the age of the animals can be measured by examining the teeth of the animals and discriminating their age. However, the teeth of the cows are different from the breed, regional location, genetic characteristics, food type, breeding environment, Since there are various individual differences according to nutritional status, it can be used as a statistical data for estimating relative age, but it cannot be used as an indicator for determining absolute age. There are also disadvantages that cannot be applied to meat in circulation.
따라서 보다 과학적이고 정확성을 가지는 동물의 월령 측정 방법의 확립이 요구되는 실정이다. Therefore, the establishment of a more scientific and accurate animal age measurement method is required.
이에 본 발명자는 상기 문제를 해결할 수 있는 동물 월령 측정 방법을 연구한 결과 생물학적 반감기를 이용하여 동물의 출생시 특정 동위원소를 농축시켜 투여한 뒤, 도축 또는 유통과정에서 동위원소간의 비를 측정함으로써 동물의 월령을 알아낼 수 있음을 발견함으로써 본 발명을 완성하게 되었다. Therefore, the present inventors have studied a method of measuring the age of the animal that can solve the above problems, and concentrated and administered a specific isotope at the birth of the animal using a biological half-life, and then measured the ratio between the isotopes in the slaughter or distribution process. The present invention has been completed by discovering that the age of.
본 발명은The present invention
동물이 출생한 뒤 바로 안정동위원소를 농축하여 출생한 동물에 투여하는 단계; 및Concentrating the stable isotope immediately after the birth of the animal and administering to the born animal; And
상기 동물의 체내에 존재하는 안정동위원소간의 질량 비율을 측정하는 단계Measuring the mass ratio between stable isotopes present in the body of the animal
를 포함하는 동위원소를 이용한 동물의 월령 측정 방법을 그 특징으로 한다. Characterized by the method of measuring the age of the animal using an isotope comprising a.
본 발명의 월령 측정 방법에 의해 종래의 치아감별법의 부정확성을 보완할 수 있으며, 출생시 일정량의 동위원소 화합물의 투여를 통해 유통 단계에서 동물의 월령을 확인할 수 있어 고령 쇠고기의 시장 유통을 관리할 수 있으며, 광우병 우려로 인한 불안을 해소하는데 이바지할 수 있다. 또한 다양한 동물에서 동위원소의 반감기를 측정함으로써 소 이외의 기타 포유 동물의 나이 측정에도 응용될 수 있다. The age measurement method of the present invention can compensate for the inaccuracies of the conventional dental differentiation method, and it is possible to check the age of the animal at the distribution stage by administering a certain amount of isotope compound at birth, thereby managing the market distribution of the aged beef. It can also help to relieve anxiety caused by mad cow disease. It can also be applied to the determination of the age of mammals other than cattle by measuring the half-life of isotopes in various animals.
본 발명은 생물학적 반감기를 이용하여 동물의 출생시 관심 원소 중 특정 동위원소를 농축시켜 투여한 이후, 도살 또는 유통과정에서 생체조직의 표본을 취하여 표본 내 관심 원소의 동위원소비를 측정함으로써 도살 시점에서의 동물의 월령을 측정하는 방법에 대한 것이다.According to the present invention, a biological half-life is used to concentrate a specific isotope of an element of interest at the time of birth of an animal, and then, after slaughtering or distribution, take a sample of biological tissue and measure the isotope ratio of the element of interest in the sample. Is about how to measure the age of an animal.
생물학적 반감기(biological half-life)는 생체 내에서 어떤 물질의 생리학적, 약리학적, 방사학적 작용 및 활동성이 절반으로 감소하는 데 걸리는 시간으로 정의된다. 몇몇 원소의 인체를 기준으로 한 생물학적 반감기가 알려져 있으며 이를 하기 표 1에 나타내었다.Biological half-life is defined as the time it takes for a physiological, pharmacological, radiological and activity of a substance to be halved in vivo. Biological half-lives based on the human body of several elements are known and are shown in Table 1 below.
먼저 월령을 측정하고자 하는 동물의 출생시에 특정 동위원소를 농축하여 동물의 체내로 투여한다. 이때 동위원소로는 방사성 동위원소를 사용하지 아니하고, 자연상에 존재하는 안정동위원소를 사용한다. First, a specific isotope is concentrated at the birth of an animal whose age is to be measured and administered into the body of the animal. At this time, do not use radioisotope but use stable isotope existing in nature.
안정동위원소란 동위원소 가운데 방사성동위원소를 뺀 나머지 원소를 말하며, 자연상태에서 동일 원소의 그 존재비는 지구상에서 일정하다.Stable isotopes are the remaining elements of the isotope minus radioisotopes. In nature, their abundance is the same on earth.
안정동위원소 중에서 바람직하게는 Fe, S 중에서 선택된 원소의 동위원소를 사용한다. Fe, S 는 동물의 체내의 특정 조직에 축적되거나 체내 농축이 일어나지 아니하므로 일정 부분의 표본만으로도 상기 동물의 체내에 존재하는 안정동위원소간의 질량 비율을 정확하게 측정할 수 있다. Among the stable isotopes, an isotope of an element selected from Fe and S is preferably used. Since Fe and S do not accumulate in specific tissues in the body of the animal or do not concentrate in the body, only a portion of the sample can accurately measure the mass ratio between stable isotopes present in the body of the animal.
동물에 투여된 동위원소는 시간이 흐르면서 생체 대사, 배설 등에 의하여 그 질량이 감소하게 되며, 시간에 따른 동위원소의 질량 비율을 측정하여 동물의 월령에 따른 동위원소의 질량비 데이터를 얻을 수 있다. 이 때 자연존재비가 낮은 안정동위원소를 농축하여 동물에 투여하는 것이 월령 측정의 정확성을 높이는데 바람직하다.Isotope administered to the animal is reduced in mass due to metabolism, excretion, etc. over time, the mass ratio data of the isotope according to the age of the animal can be obtained by measuring the mass ratio of the isotope over time. At this time, it is desirable to concentrate the stable isotope with a low natural abundance and to administer it to the animal to increase the accuracy of the age measurement.
하기 표 2는 철을 동물의 월령 측정에 사용하는 경우 농축 샘플에서의 안정동위원소의 존재비를 예시한 것이다. Table 2 below illustrates the abundance of stable isotopes in concentrated samples when iron is used to measure the age of animals.
즉 동물이 출생한 뒤 바로 안정동위원소를 농축한 샘플을 출생한 동물에 투여한 뒤, 시간의 흐름에 따라 자연존재비가 높은 특정 안정동위원소와 샘플에 농축되어 동물에 투여된 안정동위원소와의 질량 비율을 측정하여 이를 통계화한다. In other words, a sample containing a stable isotope enriched immediately after the birth of the animal was administered to the born animal, and then, with a stable isotope of high natural abundance and a stable isotope concentrated in the sample over time. Measure the mass ratio and statify it.
따라서 도축시 또는 유통단계에서 고기 내의 동위원소의 질량비를 측정하면 상기 통계화된 질량 비율에 따라 동물의 월령을 알아낼 수 있게 된다. Therefore, by measuring the mass ratio of isotopes in the meat at the slaughter or distribution stage, it is possible to determine the age of the animal according to the statistical mass ratio.
상기 안정동위원소의 질량 비율은 혈액을 채취하여 측정하거나, 동물이 도축된 뒤 표본을 채취하여 측정할 수 있다. The mass ratio of the stable isotope can be measured by taking blood or by taking a sample after the animal is slaughtered.
보다 정확한 측정을 위하여는 각 동물이 출생시 체내에 함유하고 있는 철의 양을 측정하고 그에 따라 안정동위원소가 농축된 샘플의 투여량을 결정하여 상기 샘플의 투여후 동위원소간의 질량비가 동일 개체간에 일정하게 유지되도록 함이 이바람직하다. For a more accurate measurement, the amount of iron contained in the body at the time of birth of each animal is determined, and accordingly, the dose of the stable isotope-concentrated sample is determined so that the mass ratio between the isotopes after the administration of the sample is the same. It is desirable to keep it constant.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR970007066B1 (en) * | 1992-02-03 | 1997-05-02 | 러트거어스, 더 스테이트 유니버어시티 | Method and apparatus isotopic analysis |
| KR20030079968A (en) * | 2001-01-29 | 2003-10-10 | 메타라 인코포레이티드 | Automated in-process isotope and mass spectrometry |
| US20050224001A1 (en) | 2004-04-08 | 2005-10-13 | Optibrand Ltd., Llc | Method of processing an auditable age record for an animal |
| WO2008063783A2 (en) | 2006-10-13 | 2008-05-29 | Metabolon, Inc. | Biomarkers related to metabolic age and methods using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR970007066B1 (en) * | 1992-02-03 | 1997-05-02 | 러트거어스, 더 스테이트 유니버어시티 | Method and apparatus isotopic analysis |
| KR20030079968A (en) * | 2001-01-29 | 2003-10-10 | 메타라 인코포레이티드 | Automated in-process isotope and mass spectrometry |
| US20050224001A1 (en) | 2004-04-08 | 2005-10-13 | Optibrand Ltd., Llc | Method of processing an auditable age record for an animal |
| WO2008063783A2 (en) | 2006-10-13 | 2008-05-29 | Metabolon, Inc. | Biomarkers related to metabolic age and methods using the same |
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