JP2004292151A - System and method for collection/conveyance/storage of dna sample for verifying history of meat - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a simple and inexpensive system and a method for collection/conveyance/storage of DNA sample for verifying the history of meat for forming a food distribution system for verifying the DNA of meat. <P>SOLUTION: This system comprises equipment at a production site or work shop 1, equipment at a DNA control center 2, a carrying means 3, and a DNA sample distribution and storage means 4. The DNA sample distribution and storage means 4 comprises a sample collector 41 capable of individually sealing a sampled DNA sample 40 and having a bar code 41 for identification, a sample tape 51 on which a plurality of sample collectors 41 are stuck in a vertical arrangement, and a dedicated envelope 61 capable of storing a plurality of sample tapes 51 fixed at their upper parts with a stapler and on which the bar code 41 for identification is stuck. The sample is carried to a DNA control center 2 for arrangement and storage. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a system for collecting, transporting, and storing a DNA sample for verifying meat history and a method for collecting, transporting, and storing the DNA sample.
[0002]
[Prior art]
In the past, the history of foods was mainly based on the indication of the place of origin to emphasize the characteristics of the foods.However, due to food poisoning and other damage caused by foods and ingredients, the import of foreign products increased in recent years. Consumers are increasingly interested in the history of foods, and recently, most foods have come to display the place of origin, contents, expiration date, and the like.
[0003]
For meat, in particular, the disclosure of the history is requested due to the influence of the BSE problem, and there is an increasing demand for information provision from the production area to the consumption area.
[0004]
[Patent Document 1]
WO-A1-0118239
[0005]
[Problems to be solved by the invention]
However, the labeling of the place of origin up to now is mainly a voluntary label of the producer, and since the false labeling of the place of origin has recently become a social problem, there is a strong demand for food safety and product integrity. As a new technique, DNA-based genetic testing has been partially performed.
[0006]
Especially in the case of meat, there is a strong demand for so-called traceability, which can be traced back to individuals in the production area due to the actual situation of BSE damage abroad. In some cases, ear tag numbers for individual identification in producers are used as original data. There is an opportunity to introduce a meat management method that links consumer goods to a series of identification numbers and manages them as a database. However, since this method also requires human input for intermediate input, it has been proposed to carry out genetic testing to ensure the integrity of the product. Not realized.
[0007]
SUMMARY OF THE INVENTION An object of the present invention is to provide a simple and inexpensive system for collecting, transporting and storing DNA samples for meat history verification, which constitutes a food distribution system on the premise of meat DNA verification, and a method thereof. .
[0008]
[Means for Solving the Problems]
The meat sample verification DNA sample collection / transportation / storage system of the present invention comprises:
This system collects DNA samples collected at the production and processing stages of meat circulating from the production site to the consumption site from the production site and processing site for future verification, transports them to the DNA management center, and stores them. The collected DNA samples can be individually enclosed and have a barcode for identification, a sample tape to which a predetermined number or less of sample collectors are attached in a predetermined arrangement, and one or more sample tapes can be stored and identified. A special envelope that can be pasted with a barcode for reading, a barcode of the sample collector is read, the barcode is stored in association with the history of the target meat, and attached to the dedicated envelope to identify the contents. Information processing device at the production site and processing site to create barcodes for the production, and from the production site and processing site to the DNA management center A bar that reads from the DNA sample storage for organizing and storing the sent dedicated envelope, a storage address in the DNA sample storage designated for the transferred dedicated envelope, and the dedicated envelope and the stored sample collector. The information processing device of the DNA management center is configured to store the code and the production location information in association with the production location information, and to search a storage address of a desired sample collector in response to a request for analysis and determination by DNA.
[0009]
The DNA management center may further include a drying device for drying the transported DNA sample to a predetermined degree of drying before storing it in the DNA sample storage, and the storage may be maintained at an air-conditioning temperature.
[0010]
The DNA sample may be at least one of an animal hair sample collected from a living body and a meat piece sample collected from carcass.
[0011]
The sample tape used for the above-mentioned meat history verification DNA sample collection / transportation / storage system has a structure in which the collected DNA samples are individually enclosed and a predetermined number of sample collectors having barcodes can be stuck vertically and stuck. A plurality of tapes can be fixed on the upper side of the vertically arranged sample connectors, and the bar code of the lower sample tape can be read by a bar code reader by turning the upper sample tape.
[0012]
The dedicated envelope used for the above-mentioned meat history verification DNA sample collection / transportation / storage system can store sample tapes in which the collected DNA samples are individually enclosed and each of which has a barcode attached sample collector. A sign indicating that the DNA sample is built-in on the surface and the address of the DNA management center of the transport destination are described as an address, and a barcode indicating the history of meat at the production site and the processing site and a storage address at the DNA management center are displayed. The bar code shown can be marked, and the back surface is made of a material having air permeability to facilitate drying of the contents, and has the outer dimensions corresponding to the storage shelves of the storage at the DNA management center. And
[0013]
The drying device provided in the DNA management center of the above-mentioned meat history verification DNA sample collection / transportation / storage system is a dedicated envelope for accommodating a sample tape to which a sample collector in which the collected DNA samples are individually enclosed is attached. A storage shelf capable of storing a plurality of such that the front and back surfaces are spaced from each other, an outer wall portion having a door capable of storing the storage shelf and capable of taking out and taking out a dedicated envelope in the storage shelf, and being stored in the storage shelf. And a dehumidifying and heating mechanism for dehumidifying the drying air and increasing the temperature of the drying air in a space between the dedicated envelopes. Here, it is desirable that the temperature of the drying air flowing through the space between the dedicated envelopes is 60 ° C. or less.
[0014]
The method for collecting, transporting, and storing a DNA sample for verifying meat history according to the present invention includes:
DNA samples collected at the production and processing stages of meat circulating from production to consumption are collected at the production and processing sites for future verification, transported to the DNA management center, and stored at the DNA management center. A method comprising performing, as a DNA sample, at least one of collection of animal hair samples from a living body and collection of a meat piece sample from carcass, and using the collected DNA sample as a sample having a barcode for identification. Individually enclosed in a collector, a predetermined number of created sample collectors are affixed to a sample tape in a predetermined arrangement, and a plurality of sample tapes are fixed at predetermined positions so that they can be rolled up. Reads the barcode and stores it in the information processing device at the production site or processing site in association with the history of the meat And let the information processing device create a barcode that describes the predetermined information to be attached to the dedicated envelope that stores the sample tape, store the sample tape in the dedicated envelope, and attach the created barcode to the dedicated envelope. Then, the dedicated envelope was transported to the DNA management center, and the DNA management center, which received the dedicated envelope, read the barcode of the dedicated envelope with a barcode reader, opened the dedicated envelope, and read the barcode of the internal sample collector. And store it in the information processing device of the DNA management center, determine the storage address of the dedicated envelope in the storage, create a barcode with the storage address information, affix it to the envelope, and store the DNA sample. The envelope is stored in the storage address of the storage and stored in response to a request for analysis and determination by DNA. Storage address of the desired sample collector and searching from the information processing apparatus of a DNA management center, characterized by having a step of performing a desired process removed sample collector from depot.
[0015]
Before the step of storing and storing the envelope containing the DNA sample in the storage address of the storage, storing the dedicated envelope in a drying device and drying the DNA sample to a predetermined degree of drying. Is also good.
[0016]
Since a collection / transportation / storage system and a collection / transportation / storage method of a DNA sample for verification of meat history without any intervention of a series of errors are constructed, it is possible to verify the history of meat with highly reliable DNA.
[0017]
BEST MODE FOR CARRYING OUT THE INVENTION
Next, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a schematic block diagram of a system for collecting, transporting, and storing a DNA sample for verifying meat history according to an embodiment of the present invention.
[0018]
A meat sample collection / transportation / storage system for meat history verification according to an embodiment of the present invention is a DNA sample collection / transportation / storage system for constructing a food distribution system based on meat DNA verification. In addition, DNA samples collected at the production and processing stages of meat distributed from the production site to the consumption site are collected at the production site and processing site for future verification and investigation, and DNA management, which is the core of DNA verification This system is transported to the center for long-term organization and storage.
[0019]
Normally, the storage period of meat itself is generally less than six months including frozen and chilled products, and the storage period of DNA samples is practically about six months. Considering use for selective breeding, etc., sample storage for about 5 years is desirable. It is most desirable to store the sample in a frozen state, but this requires a large amount of facility and maintenance costs. Therefore, it is desirable to dry the sample when stored at air-conditioning temperature. Is to be dried. However, if necessary, the meat may be stored frozen or the drying step may be omitted if only the DNA test is performed to verify the integrity of the distributed meat. For this purpose, drying is desirable.
[0020]
The system for collecting, transporting, and storing a DNA sample for verifying meat history according to the embodiment of the present invention includes equipment at a production site or a processing plant 1, equipment at a DNA management center 2, transport means 3, and DNA sample distribution and storage means. And 4.
[0021]
The DNA sample distribution / storage means 4 is capable of individually enclosing the collected DNA samples 40 and has a sample collector 41 having a barcode 43 for identification, and a sample tape 51 to which a plurality of sample collectors 41 are attached in a vertically arranged arrangement. And a dedicated envelope 61 on which a plurality of sample tapes 51 whose upper portions are fixed by a stapler or the like can be stored and a barcode 62 for identification is attached.
[0022]
The DNA sample 40 is one or both of an animal hair sample collected from a living body and a meat piece sample collected from carcass. Animal hair samples are usually withdrawn with a finger, and flesh samples are scraped from carcasses with a knife or a specially calibrated scraper tip.
[0023]
2A and 2B are plan views showing an example of a sample collector. FIG. 2A shows a sample collector 41a for animal hair sample, and FIG. 2B shows a sample collector 41b for meat piece sample. In this example, the DNA sample 40 is enclosed in the DNA sample enclosure 42 between the transparent adhesive film on the back surface of the sample collector 41 and the front paper. A bar code 43 is printed on the surface of the sample collector 41 in advance. In addition, an ear tag number or a slaughter number can be entered so that the production mother can be specified. This is merely an example, and the present invention is not limited to this mode, and may be any as long as the sample can be fixed and identified.
[0024]
In this example, five sample tapes 51 each having a DNA code 40 individually sealed therein and having a barcode 43 can be attached to the sample tape 51 such that the barcodes 43 are arranged in the reading direction. A plurality of sample tapes 51 can be fixed with a stapler or the like at the longitudinal end. The number of sample collectors 41 that can be attached is not limited to five, and may be changed. The reason why the bar code 43 is fixed at the front end in the longitudinal direction is that the bar code 43 of the lower sample tape 51 can be read by the bar code reader 11 by turning up the upper sample tape 51 so that the bar codes 43 are arranged in the reading direction. The purpose of this is to facilitate the operation of reading the barcode 43 of the sample collector 41 below. However, the present invention is not limited to this mode, and any mode can be used as long as the barcode 43 can be easily read.
[0025]
On the surface of the dedicated envelope 61, an indication that the DNA sample is incorporated, the destination of the DNA management center 2 at the destination are described as an address, and a bar code 62 indicating the history of meat at the production site or the processing plant 1 is marked. The sample tape 51 is stored and transported to the DNA management center 2 by the transport means 3. The DNA management center 2 can mark a bar code 63 indicating a designated storage address in the storage 24.
[0026]
The back surface of the dedicated envelope 61 is formed of a material having air permeability such as porous paper or plastic film for facilitating drying of the contents, and corresponds to a storage shelf of the DNA sample storage 23 of the DNA management center 2. It has the following external dimensions.
[0027]
The facility of the production site or the processing plant 1 stores the barcode reader 11 that reads the barcode 43 of the sample collector 41, stores the barcode 43 in association with the history of the target meat, and identifies the contents. And an information processing device 12 for creating a barcode 62 to be attached to a dedicated envelope 61.
[0028]
The transport means 3 may be mail, home delivery, or other transport means.
[0029]
The facilities of the DNA management center 2 include a DNA sample storage 24 for organizing and storing the transported dedicated envelope 61, a barcode reader 21 for reading barcodes 62 and 63 of the dedicated envelope 61 and a barcode 43 of the sample collector 41. , An information processing device 22 and a drying device 23.
[0030]
The information processing device 22 stores information read by the barcode reader 21 from the conveyed special envelope 61 and the barcodes 62 and 43 of the sample collector 41 stored in the special envelope 61 in association with production site or processing site information. At the same time, a storage address of the dedicated envelope 61 in the DNA sample storage 24 is designated, and a barcode 63 recording the storage address to be attached to the dedicated envelope 61 is created. Further, the storage address of the desired sample collector 41 is searched for in response to a request for analysis and verification by DNA.
[0031]
The drying device 23 dries the DNA sample 40 stored in the transported special envelope 61 to a predetermined degree of drying before storing it in the DNA sample storage 24.
[0032]
FIG. 3 is a schematic side sectional view of the drying device of the present embodiment. The drying device 23 is provided with a storage shelf 231 capable of storing a plurality of dedicated envelopes 61 for accommodating a sample tape 51 to which a sample collector 41 in which a DNA sample 40 is individually enclosed is stuck, so that the front and back surfaces thereof are spaced from each other. The air for drying is provided between the outer wall portion 232 having a door capable of accommodating the storage shelf 231 and storing the special envelope 61 in the storage shelf 231 and the dedicated envelope 61 stored in the storage shelf 232. And a dehumidifying and heating mechanism 235 for dehumidifying the drying air and raising its temperature. The temperature of the drying air flowing through the space between the dedicated envelopes 61 is desirably 60 ° C. or less. The drying device 23 shown in FIG. 3 is an example, and may have any structure as long as the DNA sample in the dedicated envelope 61 can be dried under predetermined conditions.
[0033]
FIG. 4 is a schematic side sectional view of the DNA sample storage of the embodiment. The DNA sample storage 24 includes a storage shelf 241 of a three-dimensional storage type capable of efficiently putting in and taking out the dedicated envelope 61 to be stored and having high storage efficiency, a storage main body 242 having an entrance door, and an air conditioner 243. . The storage shelves 241 are sectioned and assigned storage addresses. FIG. 4 also shows an example, and the present invention is not limited to this embodiment.
[0034]
Next, a method of collecting, transporting, and storing a DNA sample using the system for collecting, transporting, and storing a DNA sample for verifying meat history according to the present embodiment will be described. FIG. 5 is a flowchart showing the steps of the method for collecting, transporting, and storing a DNA sample according to the embodiment of the present invention.
[0035]
First, as a DNA sample 40 at a production site or a processing plant, one or both of collection of an animal hair sample from a living body and collection of a meat piece sample from carcass are executed (S101). Animal hair samples are usually withdrawn with a finger, and flesh samples are scraped from carcasses with a knife tip or a dedicated calibrator.
[0036]
The collected DNA samples 40 are individually enclosed in a sample collector 41 having a barcode 43 for identification (S102). As an example, the DNA sample 40 is sealed between the transparent adhesive film on the back surface of the sample collector 41 and the front sheet, and an ear tag number or a stock number is written in a predetermined place. Thereby, the production base can be specified.
[0037]
The sample collector 41 created on site is brought back to the production site or the office of the processing plant 1 (S103), and a predetermined number of the sample collectors 41 are affixed to the sample tape 51 in a predetermined arrangement (S104). In one example, five sample collectors 41 are attached to the sample tape 51 such that the barcodes 43 are arranged in the reading direction. The reason why the barcodes 43 are arranged in the reading direction is to facilitate the operation of reading the barcode 43 of the lower sample collector 41 with the sample collector 41 closed and stacked.
[0038]
A plurality of sample tapes 51 are fixed at the leading ends in the longitudinal direction by a stapler or the like (S105). The reason why the bar code is fixed at the front end in the longitudinal direction is that the bar code 43 of the lower sample tape 51 can be read by the bar code reader 11 by turning the upper sample tape 51.
[0039]
The barcode reader 11 reads the barcode 43 of the sample collector 41 and stores the barcode 43 in the information processing device 12 of the production site or the processing plant 1 in association with the history of the meat (S106). The information processing apparatus 12 creates a barcode 62 describing predetermined information to be attached (S107). When an ear tag number or a slaughter number is written in the sample collector 41, the input of this number correlates with the history of the meat.
[0040]
The bundle of the sample tapes 51 is stored in the dedicated envelope 61, and the created barcode 62 is attached to the dedicated envelope 61 (S108).
[0041]
The dedicated envelope 61 is transported to the DNA management center 2 (S109).
[0042]
At the DNA management center 2, the barcode 62 of the dedicated envelope 61 is read by the barcode reader 21 and the dedicated envelope 61 is opened, and the barcode 43 of the internal sample collector 41 is read to the information processing device 22 of the DNA management center 2. It is stored (S110). At the same time, the storage address of the dedicated envelope 61 in the DA sample storage 24 is determined, and a barcode 63 containing the storage address information is created and attached to the dedicated envelope 61 (S111).
[0043]
The dedicated envelope 61 is stored in the drying device 23, and the DNA sample 40 is dried to a predetermined degree of drying (S112).
[0044]
The dedicated envelope 61 in which the DNA sample 40 is stored is stored in the designated storage address of the storage and stored (S113), and the dedicated envelope in which the desired sample collector 41 is stored in response to the analysis determination request by DNA. The storage address of the envelope 61 is retrieved from the information processing device 22 of the DNA management center 2 (S114), the sample collector 41 is taken out of the DNA sample storage 24, and the desired processing is performed (S115).
[0045]
The drying step in step S112 can be omitted if unnecessary.
[0046]
The present invention is a DNA sample collection / transportation / storage system for constructing a food distribution system premised on DNA verification of meat, but the same system can be applied to distribution of food other than meat. .
[0047]
【The invention's effect】
As described above, the present invention standardizes the collection, transportation, and storage of DNA samples as a series of systems, and the materials used are standardized and the processing is also manualized. There is an effect that it can be incorporated into a food distribution system as an efficient and effective DNA sample collection, transport, and storage system for constructing a premise food distribution system.
[0048]
In addition, since the storage is performed in an air-conditioned atmosphere by performing a drying treatment before storage, long-term storage of DNA samples at low cost is possible, and not only verification of meat history with DNA samples but also improvement of animal species There is an effect that it can be used for improvement and selection and breeding of cows with good meat quality.
[Brief description of the drawings]
FIG. 1 is a schematic block diagram of a system for collecting, transporting, and storing a DNA sample for verifying meat history according to an embodiment of the present invention.
FIG. 2 is a plan view showing an example of a sample collector.
(A) is for animal hair samples.
(B) is for a meat piece sample.
FIG. 3 is a schematic side sectional view of the drying apparatus according to the embodiment of the present invention.
FIG. 4 is a schematic side sectional view of a DNA sample storage according to the embodiment of the present invention.
FIG. 5 is a flowchart showing steps of a method for collecting, transporting, and storing a DNA sample according to the embodiment of the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Production site or processing plant 2 DNA management center 3 Transportation means 4 DNA sample distribution storage means 11, 21 Barcode reader 12, 22 Information processing device 23 Drying device 24 DNA sample storage 40 DNA sample 41 Sample collector 41a Animal hair sample Collector 41b Meat piece sample collectors 43, 62, 63 Barcode 51 Sample tape 61 Dedicated envelope 231 Storage shelf 232 Outer wall 234 Ventilation mechanism 235 Dehumidification and heating mechanism 241 Storage shelf 242 Storage main body 243 Air conditioning equipment S101 to S115 Step

Claims (9)

This system collects DNA samples collected at the production and processing stages of meat circulating from the production site to the consumption site from the production site and processing site for future verification, transports them to the DNA management center, and stores them. hand,
A sample collector that can individually enclose the collected DNA samples and has a barcode for identification;
A sample tape for attaching a predetermined number of the sample collectors in a predetermined arrangement,
A dedicated envelope that can store one or more of the sample tapes and attach a barcode for identification;
A production area and processing for reading the bar code of the sample collector, storing the bar code in association with the history of the target meat, and creating a bar code to be attached to the dedicated envelope to identify the contents. Information processing device of the place,
A DNA sample storage for organizing and storing the dedicated envelope transferred from the production site and the processing site to the DNA management center,
A designated storage address in the DNA sample storage for the transported dedicated envelope, and a barcode read from the dedicated envelope and the stored sample collector are stored in association with production location information. Collecting, transporting, and storing a meat history verification DNA sample, comprising: an information processing device of the DNA management center that searches for a desired storage address of the sample collector in response to a request for analysis determination by DNA. system. The DNA management center further includes a drying device for drying the transported DNA sample to a predetermined degree of drying before storing it in the DNA sample storage, and the storage is maintained at an air-conditioning temperature. 2. A system for collecting, transporting and storing a DNA sample for verifying meat history according to claim 1. 2. The collection, transport, and storage system for a meat history verification DNA sample according to claim 1, wherein the DNA sample is at least one of an animal hair sample collected from a living body and a meat piece sample collected from carcass. It is a sample tape used for the collection, transportation and storage system of the DNA sample for meat history verification according to claim 1,
The collected DNA samples are individually enclosed, and a sample collector having a barcode can be stuck vertically within a predetermined number,
A plurality of sample tapes can be fixed on the upper side of the vertically arranged sample connectors, and the bar code of the lower sample tape can be read by a bar code reader by turning the upper sample tape. A sample tape characterized by the following: A dedicated envelope for use in a system for collecting, transporting, and storing a DNA sample for meat history verification according to claim 1,
Collected DNA samples are individually sealed, and each can hold a sample tape to which a sample collector with a barcode is attached,
A sign indicating that the DNA sample is built-in on the surface and the address of the DNA management center at the destination are described as an address, and a barcode indicating the history of meat at the production site and processing site and a storage address at the DNA management center are indicated. Bar code marking is possible,
The back side is formed from a breathable material to facilitate drying of the contents,
A dedicated envelope having an outer dimension corresponding to a storage shelf of a storage of the DNA management center. A drying apparatus provided in a DNA management center of the collection / transportation / storage system for a meat history verification DNA sample according to claim 2,
A storage shelf capable of storing a plurality of dedicated envelopes for accommodating a sample tape to which a sample collector in which a collected DNA sample is individually enclosed is attached, so that the front and back surfaces have an interval therebetween,
An outer wall portion capable of accommodating the storage shelf and having a door capable of storing and taking out the dedicated envelope in the storage shelf;
A blowing mechanism for flowing air for drying in the space between the dedicated envelopes stored in the storage shelf,
A dehumidifying heating mechanism for dehumidifying the air for drying and increasing the temperature thereof. The drying device according to claim 6, wherein the temperature of the drying air flowing through the space between the dedicated envelopes is 60C or less. DNA samples collected at the production and processing stages of meat circulating from production to consumption are collected at the production and processing sites for future verification, transported to the DNA management center, and stored at the DNA management center. A way to
As the DNA sample, performing at least one of collection of animal hair sample from a living body and collection of meat piece sample from carcass,
Individually enclosing the collected DNA samples in a sample collector having a barcode for identification,
A predetermined number of the created sample collectors are pasted on a sample tape in a predetermined arrangement,
A plurality of the sample tapes are fixed at predetermined positions so as to be able to be rolled up, and a barcode reader reads the barcode of the sample collector and stores the barcode in association with the history of the meat in an information processing device of a production site or a processing plant,
Causing the information processing device to create a barcode describing predetermined information for attaching to a dedicated envelope storing the sample tape,
Along with storing the sample tape in the dedicated envelope, affixing the created barcode to the dedicated envelope,
Transporting the dedicated envelope to the DNA management center,
At the DNA management center receiving the dedicated envelope, the barcode of the dedicated envelope is read by a barcode reader, the dedicated envelope is opened, and the barcode of the sample collector inside is read to process the information at the DNA management center. While storing in the device, determine a storage address in the storage of the dedicated envelope, create a barcode in which the storage address information is written, affixed to the envelope,
The envelope containing the DNA sample is stored and stored at the storage address of the storage,
Searching a storage address of the desired sample collector from the information processing device of the DNA management center in response to the request for analysis and determination by DNA, removing the sample collector from the storage, and performing a desired process. A method for collecting, transporting, and storing a DNA sample for meat history verification, comprising: Prior to the step of storing and storing the envelope containing the DNA sample at the storage address of the storage, the dedicated envelope is stored in a drying device to dry the DNA sample to a predetermined degree of drying. The method for collecting, transporting, and storing a DNA sample for verifying meat history according to claim 8, comprising a step.

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