JP2004230102A - Toxin adsorbent for humor purification column and production method therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a toxin adsorbent for a humor purification column which can prevent broken pieces from mixing into the humor during an external circulation treatment and adsorb a toxin with high efficiency. <P>SOLUTION: The characteristics of the toxin adsorbent for the humor purification column of this invention is that it is a composite fiber wherein the periphery of a component A is encompassed by a component B and a running out ratio of the component A to the surface of the fiber is 30% or less after a toxin adsorbent radical is introduced. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

【００５６】
【表２】

【００５７】
【発明の効果】
補強成分の飛び出しや繊維表面の割れを防ぐことにより体外循環治療時における体液中への割れ片混入を防止でき、かつ毒素吸着効率が高い体液浄化カラム用の繊維素材を提供することができる。[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a toxin adsorbent for a body fluid purification column for removing toxins in a body fluid, and a method for producing the same.
[0002]
[Prior art]
Bacterial infections occur with various diseases, and can then lead to serious conditions such as sepsis. Generally, bacterial infections are treated by administration of antibiotics, but in many cases, the effects are not obtained due to the condition of the patient or the drug resistance of the bacteria. For patients with low efficacy of such antibacterial agents, therapy to actively remove bacterial-derived components such as endotoxin, peptidoglycan, lipoteichoic acid, superantigen, and enterotoxin from the body using a body fluid purification column or the like is a therapeutic treatment. It is done as one.
[0003]
As a substance that adsorbs bacteria-derived components and removes them from the body, there is known a column that removes endotoxin filled with an adsorbent in which polymyxin is immobilized on polystyrene fibers (for example, see Patent Document 1).
[0004]
When chemically introducing a toxin-adsorbing group such as polymyxin directly into the fiber, the strength of the fiber itself may be reduced, but a polymer that is inert to the toxin-adsorbing group introduction reaction is used as an island component. It has been proposed to use sea-island composite fibers that are arranged and reinforced (for example, see Patent Documents 2 to 4).
[0005]
However, even when reinforced with sea-island composite fibers, island components may jump out or fiber surfaces may crack due to toxin adsorbing group introduction reaction or mechanical stress. For this reason, even if a filter is attached to the outlet of a column filled with a fiber material into which a toxin-adsorbing group has been introduced, if the fragments are small, they may slip through the filter, and there is a concern that they may be mixed into body fluids during treatment by extracorporeal circulation. I was In addition, the sea surface into which the toxin-adsorbing groups are introduced falls off due to the cracking of the fiber surface, so that the toxin-adsorbing efficiency is reduced. Therefore, it has been very difficult to control the reaction conditions and the column packing operation.
[0006]
[Patent Document 1]
JP-A-60-209525
[Patent Document 2]
JP-A-9-235263 (pages 3, 10-11)
[0008]
[Patent Document 3]
JP-A-10-147518 (pages 2, 4, 7)
[0009]
[Patent Document 4]
JP-A-10-147541 (pages 2, 4, and 6)
[0010]
[Problems to be solved by the invention]
In view of the above problems of the prior art, the present invention can prevent spalling of reinforcing components and cracking of the fiber surface, thereby preventing the incorporation of debris into body fluid during extracorporeal circulation treatment and achieving high toxin adsorption efficiency in body fluid purification. An object of the present invention is to provide a method for producing a toxin adsorbent for a column.
[0011]
[Means for Solving the Problems]
As a result of intensive studies, the present inventors have found that island components jump out due to a difference in dimensional change between a sea component and an island component during the toxin adsorbing group introduction reaction, and reached the present invention.
[0012]
To achieve the above object, the present invention has the following configuration.
(1) A composite fiber in which the component B surrounds the component A, and the composite fiber has a protrusion of the component A on the fiber surface of 30% or less after the introduction of the toxin-adsorbing group. Toxin adsorbent for body fluid purification column.
(2) A method for producing a toxin adsorbent for a bodily fluid purification column, which comprises introducing a toxin adsorbing group after subjecting a fibrous material made of a conjugate fiber in which a component A is surrounded by a component B to shrink heat treatment.
(3) The method for producing a toxin adsorbent for a body fluid purification column according to (2), wherein the shrinkage heat treatment is performed by dry heat at 60 to 150 ° C or wet heat at 40 to 100 ° C.
(4) The method for producing a toxin adsorbent for a body fluid purification column according to the above (2) or (3), wherein the composite fiber is a sea-island type composite fiber.
(5) The method for producing a toxin adsorbent for a body fluid purification column according to any one of (2) to (4), wherein the fiber material is selected from a thread, a knit, a woven fabric, and a felt.
(6) The toxin adsorbent for a body fluid purification column according to any one of (2) to (5), wherein the B component contains a polymer selected from polystyrene, polysulfone, polymethyl methacrylate, and derivatives thereof. Production method.
(7) The method for producing a toxin adsorbent for a body fluid purification column according to any one of (2) to (6), wherein the toxin adsorbing group has at least a functional group capable of forming a hydrogen bond.
[0013]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, the present invention will be described in more detail.
[0014]
The toxin adsorbent for a body fluid purification column of the present invention is a conjugate fiber in which the B component surrounds the A component, and the A component jumps out to the fiber surface after the introduction of the toxin adsorbing group at a rate of 30% or less. It is characterized by having. Since the component A jumps out of the component B, the fragments of the component B may peel off. That is, when the pop-out rate exceeds 30%, there is a high possibility that fragments of the B component will peel off, and even after washing, cracks easily enter water passed through the column. It must be: From such a viewpoint, a preferable pop-out rate is 25% or less, and a more preferable pop-out rate is 20% or less.
[0015]
In the present invention, the protrusion of the A component to the fiber surface refers to a state in which the A component is visible on the fiber surface when the fiber surface is observed with an electron microscope. It refers to the percentage of fibers that are popping out. As a specific measuring method, a toxin adsorbing material into which a toxin adsorbing group is introduced is sampled at four points, and photographed with an electron microscope at a magnification of 250 times and a substantial part having a size of 7 cm × 9 cm. One sample was photographed at random at three places so that the photographed places did not overlap, and the pop-out rates for 12 places were calculated, and the average was taken as the pop-up rate. The calculation criterion for the pop-out rate refers to the ratio of the number of fibers where the A component protrudes from the fiber surface to the total number of fibers in the photograph. In addition, the measurement can be performed by sampling as it is in a fiber form used for a woven fabric, a knitted fabric, or the like.
[0016]
In the present invention, the body fluid refers to blood, plasma, serum, ascites, lymph, joint fluid, fraction components obtained therefrom, and other liquid components derived from living organisms.
[0017]
The toxin adsorbent for a body fluid purification column of the present invention is not particularly limited, but can be obtained by the following method.
[0018]
The method for producing the toxin adsorbent for body fluid purification of the present invention is not particularly limited, but preferred examples are given below.
[0019]
The production method of the present invention is characterized in that the fiber material is subjected to a shrinkage heat treatment before the toxin-adsorbing group is introduced by a chemical reaction. By the shrinkage heat treatment, swelling and dimensional change during the toxin adsorbing group introduction reaction can be suppressed, so that the protrusion of the component A can be suppressed to 30% or less, and cracks on the fiber surface can be prevented. That is, the B component usually swells and shrinks due to the toxin adsorbing group introduction reaction, but the A component is not affected by the reaction and thus does not swell or shrink. In contrast, when the reaction is performed after the shrinkage heat treatment is performed, both the A and B components shrink to some extent by the shrinkage heat treatment, and the swelling and shrinkage of the B component due to the subsequent reaction can be suppressed. Therefore, the difference in dimensional change is reduced as a whole, and the protrusion of the A component is reduced. The shrinking process at that time is preferably performed at a shrinkage ratio of 5 to 50%, more preferably at a shrinkage ratio of 10 to 30%. If the shrinkage is too low, the difference in dimensional change between the A component and the B component after the reaction increases, and the protrusion of the A component increases, exceeding 30%. There is no particular problem that the shrinkage ratio increases, but the maximum value is limited by the properties of the material. The temperature of the heat treatment is not particularly limited, but needs to be a temperature at which the material can be thermally shrunk. Specifically, in the case of dry heat, the treatment is preferably performed at 60 to 150 ° C, more preferably. The temperature is 70 to 140 ° C, more preferably 80 to 120 ° C. In the case of wet heat, the treatment is preferably performed at 40 to 100 ° C, more preferably 60 to 100 ° C, and even more preferably 70 to 95 ° C. If the temperature is too low, shrinkage is not sufficient, and if it is too high, the polymer of the material may be deteriorated, which is not preferable. Since the processing time varies depending on the processing temperature, it can be appropriately determined according to, for example, a target shrinkage ratio. From the viewpoint of processing efficiency assuming production, the processing time is preferably 15 minutes or less, and more preferably 10 minutes or less.
[0020]
In the present invention, the shrinkage ratio is a substantial yarn shrinkage ratio, and in the case of a woven fabric or a knitted fabric, means a substantial yarn shrinkage ratio when the yarn is unraveled from a processed form of a woven fabric or a knitted fabric. It is calculated from the fiber length before and after the shrink heat treatment.
[0021]
The form of the conjugate fiber in the present invention is not particularly limited as long as the B component surrounds the A component, and a core-sheath type conjugate fiber form or a sea-island type conjugate fiber form can be preferably applied. From the viewpoint of maintaining the strength of the fiber itself and exhibiting flexibility, the sea-island composite fiber form is particularly preferable. Here, the sea-island composite fiber form is a fiber form in which at least two components are included, one component (island component) is surrounded by the other component (sea component), and the number of islands is two or more. Say That is, the strength of the B component after the introduction of the toxin adsorbing group is greatly reduced, and the strength of the A component is substantially the strength of the fiber itself. While the strength is increased, the rigidity of the fiber is increased by increasing the ratio of islands, and the fiber surface is easily cracked by mechanical stress.However, if it is a sea-island type composite fiber form, the ratio of island components is substantially the same. Even so, by increasing the number of islands and making each of the islands thinner, it is possible to maintain the strength of the fibers themselves while suppressing the improvement in the rigidity of the fibers. Further, when the number of islands further increases, the area where the island component and the sea component come into contact increases, so that the sea component is particularly difficult to peel off. From such a viewpoint, the number of islands is preferably 10 or more, more preferably 15 to 100. If the number of islands is too large, the island components become one at the time of spinning, so-called merging occurs, and the island becomes thicker.
[0022]
The form of the fiber material of the present invention is not particularly limited, but is preferably selected from yarns, knits, woven fabrics, nonwoven fabrics and felts from the viewpoint of ease of shrinkage heat treatment. Further, in consideration of the case where a toxin-adsorbing group is introduced by a reaction or the case where a column is packed, a sheet-like form such as a knitted fabric, a woven fabric, a nonwoven fabric, and a felt is particularly preferable. Of course, the above-mentioned shrinkage heat treatment does not prevent heat treatment in the state of a thread and subsequent processing into a knitted fabric, woven fabric, nonwoven fabric, felt, and the like. The effects of the present invention can be obtained. . When used as a yarn, a knitted fabric, or a woven fabric, the fiber can be used without any problem whether it is a monofilament or a multifilament, but the use of a multifilament is particularly preferable because a large number of toxin-adsorbing groups can be introduced to the total fineness. is there. From such a viewpoint, the preferred number of filaments when using a multifilament is 5 or more, and more preferably 10 or more. The total fineness of the conjugate fiber is preferably 30 dtex or more, and more preferably 40 to 300 dtex, since it is necessary to have a certain degree of strength during weaving and knitting. If it is too thick, it will be difficult to weave and knit. It is a preferable embodiment to twist the multifilament during weaving or knitting. That is, loosening and fluffing of a single yarn can be suppressed by twisting, and weaving and knitting become easy. There is no problem with the twist direction in the S direction or the Z direction, and the number of twists is not particularly limited, but the preferred number of twists is 10 t / m or more, more preferably 15 to 500 t / m, and further preferably 18 to 200 t / m. It is. When the number of twists is increased, the single yarns are excessively united, and the effect as a multifilament is reduced. The twisting set after the twisting can be preferably performed as necessary, for example, for removing residual twisting torque. In addition to the above-described embodiments, the present invention can be applied in various embodiments such as false twisted yarn and interlaced yarn. Further, regardless of the form of the fiber material, the conjugate fiber according to the present invention can be subjected to processing such as stretching, relaxation treatment, and heat treatment for the purpose of, for example, improving strength and adjusting fineness, as required.
[0023]
The single fiber fineness of the fiber material of the present invention is not particularly limited, but is preferably 20 dtex or less, more preferably 10 dtex or less, and further preferably 0.1 dtex to 7 dtex. When the fineness of the single yarn is reduced, the fiber itself becomes pliable, so that it is easy to handle even after filling the column after the reaction, it is difficult to break, and the specific surface area per column packing amount (g) is improved. This is because the toxin adsorbing group can be introduced, and the adsorbing efficiency also increases. However, if the fineness of the single yarn is too small, the fiber as a whole loses its strength and the single yarn breakage is liable to occur at the time of filling the column, which is not preferable.
[0024]
In the composite fiber of the present invention, the B component preferably accounts for 30 wt% or more, more preferably 40 wt% to 80 wt%, and even more preferably 50 wt% to 70 wt%. If the proportion occupied by the B component in the fiber is too low because the toxin adsorbing group is introduced into the B component, the toxin adsorbing group will not be sufficiently introduced and the toxin adsorption rate will decrease. On the other hand, if the proportion of the B component is too high, the proportion of the A component that plays a role of reinforcing the fiber material will decrease, so that the strength required for the fiber material cannot be maintained.
[0025]
The polymer used for the component B is not particularly limited as long as it can chemically introduce a toxin-adsorbing group. However, polystyrene, polysulfone, polymethylene methacrylate and derivatives thereof, for example, poly-α-methylstyrene, polyvinyl toluene , Polyvinyl xylene, polychloromethylstyrene, and other homopolymers, and copolymers of two or more of these or copolymers with other polymers are more preferably used. Further, natural polymers containing cellulose, collagen, chitin, chitosan and derivatives thereof are also preferably used. Further, the polymer used as the B component is not necessarily one kind, and for example, it is preferable to use a blend of the above polymer and a polymer inert to the reaction because the reinforcing effect and the crack prevention effect can be enhanced. It is.
[0026]
The polymer used as the component A is a polyester, a polyamide, a polyetherimide, a polyamide, a polyether, a polyphenylene sulfide, a homopolymer such as poly-α-olefin, or a copolymer or a blend thereof, for reinforcement. Among them, a poly-α-olefin excellent in chemical resistance is preferably used. As the poly-α-olefin, polypropylene, polyethylene, poly-3-methylbutene-1, poly-4-methyl-methylpentene-1 and the like are preferably used.
[0027]
The toxin to be removed by the body fluid purification column using the toxin adsorbent of the present invention is generally present in a large number, and is usually harmless or has a good effect even if it causes harm depending on the situation at that time. Although there is no particular limitation, for example, enterotoxin of Staphylococcus aureus, hemolytic toxin, leucocidin, coagulase and protein A, cholera toxin of cholera, velotoxin produced by hemorrhagic Escherichia coli and Shigella, Exotoxin A produced by Pseudomonas aeruginosa, secreted toxins such as pyrogenic exotoxin produced by streptococci, compounds that constitute bacteria themselves such as lipopolysaccharide, and deoxyribonucleic acid that is a genetic component of bacteria, Further, cytokines such as TNF-α and IL-6 are also toxins. Furthermore, among these are a group of toxins called pyrogens. Pyrogens are a general term for a group of substances having a pyrogenic property, and are highly toxic substances known to induce fever and shock symptoms by invading the body through bacterial infection. Examples of the toxin having pyrogen activity include lipopolysaccharide (endotoxin), which is a cell membrane component of Gram-negative bacteria, enteric toxin of Staphylococcus aureus, and Toxic shock syndrome toxin-1.
[0028]
The toxin adsorbing group to which the adsorbent of the present invention can exert its effect is not particularly limited as long as it has high adsorption performance to toxins, but preferably has a hydrogen-bonding functional group, and preferably has a hydrogen-bonding functional group. The group preferably has a substituent selected from a urea bond, a thiourea bond, an amide bond, an amino group and a hydroxyl group, and more preferably has a functional group capable of forming a hydrophobic bond. There is no particular limitation on the substituent having a urea bond, a thiourea bond or an amide bond, and an aliphatic compound such as a hexyl group, an octyl group, or a dodecyl group or an aliphatic compound such as a cyclohexyl group or a cyclopentyl group, and more preferably a phenyl group And an aromatic compound such as a naphthyl group and an anthracyl group. Further, derivatives such as aminohexyl group, monomethylaminohexyl group, dimethylaminohexyl group, aminooctyl group, aminododecyl group, tolyl group, chlorophenyl group, nitrophenyl group, diphenylmethyl group, and amine diphenylmethyl group are also preferably used. . As the compound having an amino group, for example, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, dipropylenetriamine, polyethyleneimine, N-2,2-diaminodiethylamine and the like are preferably used. Examples of the compound having a hydroxyl group include compounds such as hydroxypropane, 1,3-diamino-2-hydroxypropane, hydroxybutane, hydroxybutyric acid, and hydroxypyridine, and glucose, glucosamine, galactosamine, maltose, cellobiose, sucrose, agarose, and cellulose. Monosaccharides, oligosaccharides and polysaccharides such as chitin and chitosan, and saccharides or derivatives thereof are preferably used as substituents. Examples of the compound having an ether bond include 1,2-bis (2-aminoethoxy) ethane and 3,9bis (3-aminopropyl) -2,4,8,10-tetraoxaspiro [5. 5] Undecane or the like can be used. Further, those having a functional group capable of forming a hydrogen bond, such as a carboxyl group, a mercapto group, a urethane group, a guanidino group, and an ester group, are also suitably used as the functional group.
[0029]
The method of introducing the toxin-adsorbing group into the fiber material is not particularly limited, but it can be preferably introduced by one-step or multi-step operation by chemical reaction, coating, or other methods depending on the type of toxin-adsorbing group. Further, the timing of introducing the toxin-adsorbing group may be before or after forming into a sheet form such as a woven or knitted fabric. As an example, when introducing a toxin adsorbing group having an amino group, first, a reactive functional group that can be immobilized by a covalent bond with the amino group is introduced into the polymer of the B component by a chemical reaction or the like, and then, the functional group A method is preferred in which the amino group of the toxin adsorbing group is covalently bonded and introduced. Further, as long as the toxin adsorbing group has a functional group covalently bonded to an amino group, it is also a preferable method to react with the introduced amino group to introduce the toxin adsorbing group.
[0030]
There are various methods for measuring the toxin adsorption rate, and the removal rate may vary depending on the difference, for example, the volume ratio between the adsorbent and the liquid to be adsorbed, the concentration of impurities in the liquid to be adsorbed, the reaction time, the presence or absence of stirring, and the like. Can be The measurement of the adsorption rate in the present invention is shown below.
[0031]
The measurement of the adsorption rate of bacterial components and pharmaceuticals such as antibiotics was performed by measuring bovine serum albumin (fraction V) so that the concentration was 5 mg / ml in 0.1 M phosphate buffer (pH 7.4) containing 0.15 M sodium chloride. ) Is used. The volume ratio was determined by adding 0.1 g of the adsorbent to 1 ml of the liquid to be adsorbed, the reaction temperature was 37 ° C., the adsorption time was 2 hours, and the stirring was performed under swirling conditions. From these results, the adsorption rate was calculated using the following equation (1).
(C0−C) / C0 (1) where C0 is the initial concentration of the substance to be adsorbed in the solution before the adsorption, and C is the concentration of the substance to be adsorbed in the solution after the adsorption. .
[0032]
The column for purifying body fluid of the present invention can be produced by packing the column with the toxin adsorbent produced by the method of the present invention. The column is composed of a toxin adsorbent formed in a flat plate shape, a column filled with the toxin adsorbent, and a cylindrical filter formed by winding the toxin adsorbent in a cylindrical shape, with a blood inlet and a blood outlet at both ends. A column contained in a cylindrical container having a hollow cylindrical filter in which a toxin adsorbent is wrapped in a cylindrical shape is placed in a cylindrical container having a blood inlet and a blood outlet with both ends sealed. Preferably, the blood inlet of the container is a column provided at a portion communicating with the outer peripheral portion of the hollow cylindrical filter, and the blood outlet of the container is preferably a column provided at a portion communicating with the inner peripheral portion of the hollow cylindrical filter.
[0033]
【Example】
Hereinafter, the present invention will be described more specifically with reference to experimental examples.
[0034]
Example 1
A sea-island composite fiber (number of islands: 16) composed of 35 wt% of an island component (polypropylene) and 65 wt% of a sea component (polystyrene) was discharged at a rate of 14.4 g / min and a take-up speed of 1600 m / using a two-component spinning apparatus. Minutes. The resulting fiber had a single yarn fineness of 4.5 dtex and a filament count of 18. Two of these fibers were combined and twisted (S33 t / m, 162 dtex, 36 filaments) to produce a tubular knitted fabric. The obtained knitted fabric was heat-treated at 110 ° C. for 5 minutes in a hot-air dryer. In this treatment, the length shrank by 25% and the width shrank by 5%. When the yarn was released and the shrinkage of the yarn was examined, the shrinkage was 26%.
[0035]
The knitted fabric was reacted with a mixed solution of 50 g of N-methylol-α-chloroacetamide, 400 g of nitrobenzene, 400 g of 98% sulfuric acid, and 0.85 g of paraformaldehyde at 20 ° C. for 1 hour. Thereafter, the knitted fabric is washed with nitrobenzene, the reaction is stopped with water, and then washed again with methanol to obtain an α-chloroacetamidomethylated crosslinked polystyrene knitted fabric (hereinafter abbreviated as AMPSt knitted fabric). .
[0036]
Next, 20 g of AMPSt knitted fabric was added to a solution of 6.3 g of tetraethylenepentamine and 7.2 g of n-butylamine dissolved in 500 ml of dimethyl sulfoxide (hereinafter abbreviated as DMSO) with stirring. The reaction was carried out at 30 ° C. for 3 hours. Thereafter, the reacted knitted fabric was washed on a glass filter using 500 ml of DMSO, and the knitted fabric was added to a solution of 2.3 g of 4-chlorophenylisocyanate in 500 ml of DMSO. The reaction was carried out at 25 ° C. for 1 hour. Thereafter, the resultant was washed with 1000 ml of DMSO on a glass filter to obtain an AMPSt knitted fabric into which a urea bond was introduced (hereinafter abbreviated as UAMP knitted fabric).
[0037]
Using the produced UAMP knitted fabric, an adsorption test of a component derived from bacteria was performed. Toxic shock syndrome toxin-1 (hereinafter abbreviated as TSST-1; manufactured by Toxin Technology), protein A (hereinafter abbreviated as PrA, manufactured by Zymet), α-hemolysine, which is an exotoxin produced by Staphylococcus aureus, includes bacterial components. (Hereinafter abbreviated as aHL; purchased from Research Biochemical Institute), a protease (hereinafter abbreviated as SPEB, manufactured by Toxin Technology), lipopolysaccharide derived from Escherichia coli, and E. coli. coli0111B4 (hereinafter abbreviated as LPS, manufactured by Sigma) was used. The concentration was measured by enzyme immunoassay for TSST-1, PrA, aHL, and SPEB, and by Limulus reagent (manufactured by Wako Pure Chemical Industries) for LPS.
[0038]
0.15 M sodium chloride, 5 mg / ml bovine serum albumin (fraction V) (Seikagaku Corporation) containing TSST-1, PrA, aHL, SPEB at 1 ng / ml and LPS at 10 ng / ml. It was dissolved in a 1 M sodium phosphate buffer (pH 7.4) to obtain a liquid to be adsorbed. 0.1 g of UAMP was added to 1 ml of the liquid to be adsorbed, and the adsorbing reaction was carried out while rotating and stirring at 37 ° C. for 2 hours. Table 1 shows the results of measuring the concentration before and after the adsorption and calculating the removal rate using the above-mentioned equation (1). High removal performance of 50% or more against bacterial components.
[0039]
The state of the fiber surface of the obtained UAMP knitted fabric was observed with an electron microscope, and although the fiber surface was uneven, almost no island component jumping out or cracking occurred, and the jumping out rate was 5.7%. there were.
[0040]
Example 2
The tubular knitted fabric produced in Example 1 was treated with a hot air dryer at 80 ° C. for 15 minutes. In this treatment, the length shrinks by 10% and the width shrinks by 1%. When the yarn was unscrewed and the shrinkage was measured, it was 11%.
[0041]
UAMP fibers were produced from the heat-treated tubular knitted fabric in the same manner as in Example 1, and an adsorption test was performed. As a result, high removal performance was exhibited as shown in Table 1.
[0042]
When the fiber surface state was observed with an electron microscope, the pop-out ratio was 27.8%.
[0043]
Example 3
The tubular knitted fabric prepared in Example 1 was treated in a hot water bath at 90 ° C. for 10 minutes. In this treatment, the length shrank by 15%, and the width shrank by 2%.
[0044]
UAMP fibers were prepared from the heat-treated tubular knitted fabric in the same manner as in Example 1, and an adsorption test was performed. As a result, high removal performance was exhibited as shown in Table 1.
[0045]
Further, when the surface state of the fiber was observed by an electron microscope, almost no protrusion or cracking of the island component occurred as in Example 1, and the protrusion ratio was 6.3%.
[0046]
Example 4
The sea-island type composite fiber spun in Example 1 was passed through a contact heater (length 1 m) at 180 ° C. at a yarn processing speed of 150 m / min and an overfeed rate of 30% to be treated. In this treatment, the shrinkage was 20% and the total fineness was 101 dtex. Two of these fibers were combined and twisted (S33 t / m, 202 dtex, 36 filaments) to produce a tubular knitted fabric.
[0047]
UAMP fibers were produced on the knitted fabric in the same manner as in Example 1, and an adsorption test was performed. As a result, high removal performance was exhibited as shown in Table 1.
[0048]
When the fiber surface state was observed with an electron microscope, almost no protrusion or cracking of the island component occurred, as in the case of Example 1, and the protrusion ratio was 14.9%.
[0049]
Comparative Example 1
A UAMP knitted fabric was produced in the same manner as described above without heat treatment of the tubular knitted fabric produced in Example 1. Table 1 shows the results of the adsorption test of the obtained knitted fabric. The removal performance was lower than that of the example.
[0050]
In addition, observation of the surface state by an electron microscope revealed that a large amount of polypropylene as an island component was exposed, and the surface was cracked or peeled off in some places around the protruding portion. The pop-out rate in this case was 76.3%.
[0051]
Comparative Example 2
The tubular knitted fabric prepared in Example 1 was subjected to a constant-length heat treatment for 5 minutes using a hot air dryer at 60 ° C. UAMP fibers were produced from this knitted fabric in the same manner as in Example 1, and an adsorption test was performed. As a result, as shown in Table 1, the removal performance was lower than that of the example.
[0052]
In addition, when the surface state of the fiber was observed by an electron microscope, the number of fibers from which the polypropylene had protruded was larger than that in the example, and the protruding rate was 35.2%.
[0053]
Examples 5 to 8, Comparative Examples 3 and 4
Each of 50 g of the UAMP knitted fabrics obtained in Examples 1 to 4 and Comparative Examples 1 and 2 was wound into a cylindrical shape to form a cylindrical filter, which was placed in a cylindrical container having a bodily fluid inlet and a bodily fluid outlet at both ends. A body fluid purification column was prepared for each. The obtained column was measured for the number of fine particles by the method described below, and the results are shown in Table 2. In the column of Example, the amount of fine particles was small and decreased with the passage time. On the other hand, in the column of Comparative Example, the amount of fine particles was large and even after 4 hours, quite a lot of fine particles were generated. Was inappropriate.
(Method of measuring the amount of spilled fine particles)
In order to investigate whether or not there is a risk that splinters may enter the body fluid during extracorporeal circulation treatment,
The fine particles flowing out when passed through the body fluid purification column were measured. The measuring method is as follows.
[0054]
Using a body fluid pump, a body fluid purification column was used to filter the filtered water of a membrane filter having a particle count of 5 μm or more with 5 particles / ml or less and a particle size of 25 μm or more with 0.01 particles / ml at a flow rate of 200 ml / min. Throughout the time, the effluent was collected 1 hour, 2 hours, 3 hours, and 4 hours after the start of the liquid sending. The number of foreign particles having a particle size of 5 μm or more and 25 μm or more contained in each liquid was measured. did.
[0055]
[Table 1]

[0056]
[Table 2]

[0057]
【The invention's effect】
By preventing protrusion of the reinforcing component and cracking of the fiber surface, it is possible to prevent the mixing of cracks in body fluid during extracorporeal circulation treatment, and to provide a fiber material for a body fluid purification column having high toxin adsorption efficiency.

Claims (7)

A bodily fluid purification column comprising a composite fiber in which the component A surrounds the component B, wherein the component A jumps out to the fiber surface after introduction of the toxin adsorbing group by 30% or less. For toxin adsorbent. A method for producing a toxin adsorbent for a body fluid purification column, which comprises introducing a toxin adsorbing group after subjecting a fibrous material composed of a conjugate fiber in which an A component is surrounded by a B component to heat treatment. The method for producing a toxin adsorbent for a body fluid purification column according to claim 2, wherein the shrinkage heat treatment is performed by dry heat at 60 to 150C or wet heat at 40 to 100C. The method for producing a toxin adsorbent for a body fluid purification column according to claim 2 or 3, wherein the conjugate fiber is a sea-island type conjugate fiber. The method for producing a toxin adsorbent for a body fluid purification column according to any one of claims 2 to 4, wherein the fiber material is selected from a thread, a knit, a woven fabric, a nonwoven fabric, and a felt. The method for producing a toxin adsorbent for a body fluid purification column according to any one of claims 2 to 5, wherein the component B contains a polymer selected from polystyrene, polysulfone, polymethyl methacrylate, and derivatives thereof. The method for producing a toxin adsorbent for a body fluid purification column according to any one of claims 2 to 6, wherein the toxin adsorption group has at least a functional group capable of forming a hydrogen bond.