JP2004191959A5 - - Google Patents

Claims (5)

In a microscopic image capturing apparatus that forms a high-resolution overall image by dividing an overall image captured at a low magnification and obtained in a field of view into small sections, and shooting and pasting each of these small sections at a high magnification. ,
A height coordinate acquisition position setting unit for setting a plurality of positions to obtain the height coordinate from the lattice underlying sampling image definitive the lattice to form a small compartment,
A coordinate reading unit that reads the height coordinate, which is the focal position of the sample in the horizontal coordinate at high magnification,
With height coordinate data read by the coordinate reading unit at a set lattice above the height coordinate acquiring positioning unit, the focus correction position calculating unit for calculating a height position at an arbitrary position in the cubicle When,
A microscope image photographing device comprising: In a microscopic image capturing apparatus that forms a high-resolution overall image by dividing an overall image captured at a low magnification and obtained in a field of view into small sections, and shooting and pasting each of these small sections at a high magnification. ,
A sample image section extraction unit that extracts a small section in which a sample image exists from a plurality of small sections;
An automatic focusing unit that automatically detects the focal position following changes in the sample image;
With
The automatic focusing unit starts detection of the focal position when moving horizontally to a small section where the sample image extracted by the sample image section extracting unit exists, and the focal point when moving horizontally to a small section where the sample image does not exist. A microscope image photographing apparatus characterized in that photographing at a high magnification is performed by stopping position detection. Take a wide-field image of the entire observation slide with a low-magnification objective lens,
Extract a region with a specimen image from this wide-field image,
The focal position is set to use real-time AF when the unevenness of the sample is large or the sample is scattered in the field of view based on the presence / absence information of the sample image obtained by the process of extracting a region with this sample image Take a high-magnification image while correcting
When the specimen is flat and widely present in the entire field of view based on the presence / absence information of the specimen image obtained by the process of extracting a region with the specimen image, a high-magnification image is taken by setting so that real-time AF is not used. ,
A method for taking a microscopic image. Extract the area where the specimen image exists from the image of the whole specimen,
Set a plurality of horizontal positions for obtaining the height coordinate Z from the extracted sample image area,
A coordinate reading unit that reads the height coordinate that is the focal position at the set horizontal position;
Using the coordinates of the set horizontal position and the height coordinate data read at that horizontal position, calculate the correction position of the focus at any position in the sample image area,
Move the height of the sample to the calculated corrected focus position when moving the sample horizontally,
A method for correcting a focal position of a microscope image photographing apparatus. Extract the area where the specimen image exists from the image of the whole specimen,
When moving horizontally to the position where the extracted sample image exists, the automatic focus position detection operation that follows the horizontal movement of the sample is started,
A focus position correction method for a microscope image photographing apparatus, characterized in that the automatic focus position detection operation is stopped when moving horizontally to a position where a sample image does not exist.