JP2004143154A5 - - Google Patents

Description

Test Example Test method: The eye drops of Examples 1, 2, 3, 4 , 7 , 8, and 9 and the eye drops of Comparative Examples 1 to 4 shown in Table 2 were used as samples. Thirty guinea pigs sensitized with cedar pollen were divided into groups of 8 to 13 animals so that allergic symptoms of the eyes 30 minutes after the cedar pollen induction were uniform. 10 μL of each sample was instilled into both eyes of a guinea pig, and 5 minutes later, 0.5 mg of cedar pollen (10 μL of a saline solution) was instilled into both eyes. In the non-instilled group, only cedar pollen was instilled. The state of the keratoconjunctiva 30 minutes after administration of the cedar pollen was visually observed. The evaluation of the keratoconjunctival condition was scored according to the Draize method according to the Draize method, with redness scored in three stages of 0-2, edema scored in five stages of 0-4, and secretions scored in four stages of 0-3 (total score of 9). did. The evaluation of each state is shown below. From this score, the improvement rate (%) of allergic symptoms was calculated by the following formula, and shown in Table 3.
Improvement rate (%) = 100 − [(average value of total score of eye drop group) / (average value of total score of non-drop group) × 100]
Redness 0: No congestion is observed at all. 1: Hyperemia is observed at a part of conjunctiva. 2: Edema is observed at entire conjunctiva. 0: No edema is observed. 1: Light edema is observed at a part of conjunctiva. : Edema covers the entire conjunctiva 3: Edema covers part of the cornea 4: Secretion in which edema covers more than half of the cornea 0: No secretion is observed 1: Clear tear-like secretion Objects are observed 2: Viscous secretions are observed 3: Secretions are film-like and cover eyelids