JP2004049003A5 - - Google Patents

Claims (76)

A peptide, its amide, or its ester or its, characterized by containing an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 1, in which the N-terminal amino acid residue may be brominated salt. The peptide, its amide, its ester or its salt according to claim 1, comprising an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 1. The peptide, its amide, its ester, or its salt of Claim 1 which consists of an amino acid sequence represented by sequence number: 1, sequence number: 2, sequence number: 3 or sequence number: 66. The peptide according to claim 1, comprising an amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 66, wherein the N-terminal tryptophan residue is 6-brominated. Or an ester or a salt thereof. A peptide, its amide, or its ester or its, characterized by containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 4 in which the N-terminal amino acid residue may be brominated salt. The peptide, its amide, its ester, or its salt of Claim 5 characterized by containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 4. The peptide, its amide, its ester, or its salt of Claim 5 which consists of an amino acid sequence represented by sequence number: 4, sequence number: 5, sequence number: 6 or sequence number: 67. The peptide, its amide, its ester, or its salt of Claim 5 which consists of an amino acid sequence represented by sequence number: 67 whose N-terminal tryptophan residue is 6-brominated. 6. The peptide according to claim 5, comprising an amino acid sequence represented by SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 67, wherein the N-terminal tryptophan residue is 6-brominated. Or an ester or a salt thereof. A peptide, its amide, its ester or its, characterized by containing an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 7 in which the N-terminal amino acid residue optionally brominated salt. The peptide, its amide, its ester, or its salt of Claim 10 characterized by containing the same or substantially the same amino acid sequence as the amino acid sequence represented by sequence number: 7. 11. The amino acid sequence represented by SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 68 or SEQ ID NO: 69. Peptide, amide thereof, ester thereof or salt thereof. A peptide, its amide, its ester or its, characterized by containing an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 13 in which the N-terminal amino acid residue may be brominated salt. The peptide, its amide, its ester or its salt according to claim 13, which contains the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 13. The peptide, its amide, its ester, or its salt of Claim 13 which consists of an amino acid sequence represented by sequence number: 13, sequence number: 14, sequence number: 15 or sequence number: 70. A peptide, its amide, its ester or its, characterized by containing an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 16 in which the N-terminal amino acid residue optionally brominated salt. The peptide, its amide, its ester or its salt according to claim 16, which contains the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 16. The peptide, its amide, its ester, or its salt of Claim 17 which consists of an amino acid sequence represented by sequence number: 16, sequence number: 17, sequence number: 18 or sequence number: 71. The peptide according to claim 1 to 18, which has an ability to bind to a protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 or SEQ ID NO: 86, or a salt thereof, Amides or their esters or their salts. The peptide, its amide, or its ester according to claim 1-18, which has the ability to bind to a protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 or a salt thereof. Or its salt. The peptide according to claim 1 to 18, which has an ability to bind to a protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 84 or SEQ ID NO: 88 or a salt thereof, Amides or their esters or their salts. A partial peptide of the peptide according to any one of claims 1 to 21, an amide thereof, an ester thereof or a salt thereof. The precursor peptide of the peptide in any one of Claims 1-21, its amide, its ester, or its salt. 24. The precursor peptide, its amide, its ester or its salt according to claim 23, comprising an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 19. The peptide of claim 24, its amide, its ester or its salt, which consists of the amino acid sequence represented by SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21 or SEQ ID NO: 72. 24. The peptide, its amide, its ester or its salt according to claim 23, comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 22. 27. The peptide of claim 26, its amide, its ester or its salt, which consists of the amino acid sequence represented by SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 or SEQ ID NO: 73. A polynucleotide comprising a polynucleotide encoding the peptide according to any one of claims 1 to 21. SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 74, SEQ ID NO: 75, The polynucleotide according to claim 28, comprising the base sequence represented by SEQ ID NO: 76, SEQ ID NO: 77, SEQ ID NO: 78, or SEQ ID NO: 79. A polynucleotide comprising a polynucleotide encoding the partial peptide according to claim 22. A polynucleotide comprising a polynucleotide encoding the precursor peptide according to claim 23. 32. The base sequence represented by SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 80, or SEQ ID NO: 81. Polynucleotides. 33. The polynucleotide of claims 28-32, which is DNA. A recombinant vector containing the polynucleotide according to any one of claims 28 to 33. A transformant transformed with the recombinant vector according to claim 34. The transformant according to claim 35 is cultured to produce the peptide according to any one of claims 1 to 21, its partial peptide, or its precursor peptide. A method for producing the described peptide, a partial peptide thereof or a precursor peptide thereof or a salt thereof The antibody with respect to the peptide in any one of Claims 1-21, its partial peptide, its precursor peptide, its amide, its ester, or its salt. The antibody according to claim 37, which is a neutralizing antibody that inactivates the activity of the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt. A medicament comprising the antibody according to claim 37. 40. The medicament according to claim 39, which is a prophylactic / therapeutic agent for obesity or hyperphagia. A diagnostic agent comprising the antibody according to claim 37. The diagnostic agent according to claim 41, which is a diagnostic agent for anorexia nervosa, obesity or hyperphagia. A medicament comprising the peptide according to any one of claims 1 to 21, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof. 44. The medicament according to claim 43, which is a preventive / therapeutic agent for anorexia nervosa or an appetite enhancer. A medicament comprising the polynucleotide according to claim 28. 46. The medicament according to claim 45, which is a preventive / therapeutic agent for anorexia nervosa or an appetite enhancer. A diagnostic agent comprising the polynucleotide according to claim 28. 48. The diagnostic agent according to claim 47, which is a diagnostic agent for anorexia nervosa, obesity or hyperphagia. A polynucleotide comprising a base sequence complementary to the polynucleotide of claim 28 or a part thereof. A medicament comprising the polynucleotide according to claim 49. 51. The medicament according to claim 50, which is a prophylactic / therapeutic agent for obesity or hyperphagia. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof according to any one of claims 1 to 21, wherein the amino acid sequence is the same as or substantially the same as the amino acid sequence represented by SEQ ID NO: 49 A method for screening a compound or a salt thereof that changes the binding property to a protein or a salt thereof containing the same amino acid sequence. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a salt thereof, the peptide according to any one of claims 1 to 21, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof, and the same or substantially the same as the amino acid sequence represented by SEQ ID NO: 59 A method for screening a compound or a salt thereof that changes the binding property to a protein or a salt thereof containing the same amino acid sequence. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 84 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof according to any one of claims 1 to 21, wherein the salt is the same as or substantially the same as the amino acid sequence represented by SEQ ID NO: 84 A method for screening a compound or a salt thereof that changes the binding property to a protein or a salt thereof containing the same amino acid sequence. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof, or a salt thereof, and the amino acid sequence represented by SEQ ID NO: 49 Alternatively, a kit for screening a compound or a salt thereof that changes the binding property to a protein containing substantially the same amino acid sequence or a salt thereof. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof, and the amino acid sequence represented by SEQ ID NO: 59 Alternatively, a kit for screening a compound or a salt thereof that changes the binding property to a protein containing substantially the same amino acid sequence or a salt thereof. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 84 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof, or a salt thereof, and the amino acid sequence represented by SEQ ID NO: 84 Alternatively, a kit for screening a compound or a salt thereof that changes the binding property to a protein containing substantially the same amino acid sequence or a salt thereof. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a compound or a salt thereof that changes the binding property with the salt;
A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a compound that changes the binding property with a salt thereof or a salt thereof; or the peptide according to any one of claims 1 to 21, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof and SEQ ID NO: 84 A compound comprising a compound or a salt thereof that changes the binding property to a protein containing the same or substantially the same amino acid sequence as the amino acid sequence or a salt thereof. A protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 or a salt thereof; a protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 Or a salt thereof; or a protein comprising an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 84 or a salt thereof; A protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 49 or a salt thereof; a protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 59 Or a salt thereof; or a protein comprising an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 84 or a salt thereof; An agent for preventing or treating anorexia or an appetite enhancer comprising the agonist according to claim 59. A preventive / therapeutic agent for obesity or hyperphagia, comprising the antagonist according to claim 60. A peptide encoding the peptide according to any one of claims 1 to 21, a partial peptide thereof, or a DNA encoding the precursor peptide thereof is used. A method for screening a compound or a salt thereof that changes the expression level of a precursor peptide. The peptide according to any one of claims 1 to 21, containing the peptide encoding the peptide, the partial peptide thereof or the precursor peptide thereof, or the peptide according to any one of claims 1 to 21, or the partial peptide thereof A kit for screening a compound or a salt thereof that changes the expression level of the precursor peptide. A medicament comprising a compound or a salt thereof that changes the expression level of the peptide according to any one of claims 1 to 21, its partial peptide, or its precursor peptide. An agent for preventing or treating anorexia or an appetite enhancer comprising a compound or a salt thereof that increases the expression level of the peptide according to any one of claims 1 to 21, its partial peptide or its precursor peptide. A prophylactic / therapeutic agent for obesity or hyperphagia comprising a compound or a salt thereof that decreases the expression level of the peptide according to any one of claims 1 to 21, its partial peptide or its precursor peptide. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 86 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof according to any one of claims 1 to 21, wherein the amino acid sequence is the same as or substantially the same as the amino acid sequence represented by SEQ ID NO: 86 A method for screening a compound or a salt thereof that changes the binding property to a protein or a salt thereof containing the same amino acid sequence. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 88 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof according to any one of claims 1 to 21, wherein the salt is the same as or substantially the same as the amino acid sequence represented by SEQ ID NO: 88 A method for screening a compound or a salt thereof that changes the binding property to a protein or a salt thereof containing the same amino acid sequence. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 86 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof, or a salt thereof, and the amino acid sequence represented by SEQ ID NO: 86 Alternatively, a kit for screening a compound or a salt thereof that changes the binding property to a protein containing substantially the same amino acid sequence or a salt thereof. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 88 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a peptide thereof, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof, and the amino acid sequence represented by SEQ ID NO: 88 Alternatively, a kit for screening a compound or a salt thereof that changes the binding property to a protein containing substantially the same amino acid sequence or a salt thereof. A protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 86 with the peptide according to any one of claims 1 to 21, its partial peptide, its amide, its ester, or its salt Or a compound that changes the binding property with a salt thereof or a salt thereof; or a peptide according to any one of claims 1 to 21, a partial peptide thereof, an amide thereof, an ester thereof or a salt thereof and SEQ ID NO: 88 A compound comprising a compound or a salt thereof that changes the binding property to a protein containing the same or substantially the same amino acid sequence as the amino acid sequence or a salt thereof. A protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 86 or a salt thereof; or containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 88 A pharmaceutical comprising an agonist of protein or a salt thereof. A protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 86 or a salt thereof; or containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 88 A pharmaceutical comprising an antagonist of protein or a salt thereof. An agent for preventing / treating anorexia or an appetite enhancer comprising the agonist according to claim 73. A preventive / therapeutic agent for obesity or hyperphagia, comprising the antagonist according to claim 74.