JP2003335700A - Mouthwash - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To prepare a mouthwash capable of solving problems that conventional mouthwashes which contain an antibacterial agent, such as listerine, are used only for the purpose of merely cleansing the oral cavity so as to be weak in the antifungal activity against fungi in the oral cavity, and further that amphotericin B syrup which is used for improving disease in the oral cavity, such as periodontal diseases, is not expected to have recovering effects on all over the body from the diseases and normalize the biofunctions. <P>SOLUTION: This mouthwash contains an antifungal antibiotic as an active ingredient. A polyene macrolide-based antifungal antibiotic is preferably used as the antifungal antibiotic. At least one of amphotericin B and nystatin is preferably contained in the mouthwash as the polyene macrolide-based antifungal antibiotic. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a mouthwash,
More specifically, it relates to a mouthwash capable of not only relieving oral disease but also improving systemic diseases caused by Candida and normalizing biological functions.

[0002]

2. Description of the Related Art Conventionally, the problems to be solved by the invention
Gargling is performed in order to prevent oral diseases or virus such as influenza from adhering to mucosal cells.
As the mouthwash, a mouthwash containing an antibacterial agent such as listerin and a drug for preventing the adhesion of virus to adherent cells are used.

Recently, it is known that an effect of improving periodontal disease is exhibited by swallowing all at once after gargling with an antifungal agent, amphotericin B syrup. In this case, for example, two amphotericin B syrups (amphotericin B concentration = 100 mg / m 2
(1:24 cc / piece) diluted with purified water to 500 cc is used. In addition, the amphotericin B syrup is effective in improving periodontal disease by swallowing it all at once after applying the stock solution to the toothbrush to brush the teeth.

However, conventional mouthwashes containing an antibacterial agent such as listerine are merely used for the purpose of cleaning the oral cavity and have a weak antibacterial activity against fungi in the oral cavity.

Amphotericin B syrup is used for the purpose of recovering oral diseases such as periodontal disease, and is expected to be effective for the recovery of diseases throughout the body or normalization of biological functions. Was not.

The present invention has been made to solve the above problems, and by gargle, fungi and pathogenic bacteria such as Candida spp. In the digestive tract from the oral cavity to the throat and further to the anus are reduced. , Not only healing of oral diseases such as periodontal disease, but also healing of the entire digestive tract, immunity or resistance, and recovery of various systemic diseases caused by fungi such as Candida spp. The purpose is to provide a mouthwash that can achieve normalization.

[0007]

Means for Solving the Problems The present inventor has found that by reducing or sterilizing a fungus belonging to the genus Candida genus distributed in the oral cavity, the oral candidiasis is recovered and the It was found that the invasion of fungi belonging to the genus Candida into the digestive organs leading to the can be prevented, the biological functions of the digestive organs extending from the oral cavity to the anus are restored, and abnormalities of other organs can be normalized. The present invention has been completed.

The present invention has been made based on the above findings, and the mouthwash according to claim 1 of the present invention is characterized by containing an antifungal substance as an active ingredient.

The mouthwash according to claim 2 of the present invention is characterized in that, in the invention according to claim 1, an antifungal antibiotic is contained as an active ingredient.

Further, the mouthwash according to claim 3 of the present invention is characterized in that, in the invention according to claim 2, the antifungal antibiotic is a polyene macrolide antifungal antibiotic. To do.

The gargling agent according to claim 4 of the present invention is the invention according to claim 3, wherein the polyene macrolide antifungal antibiotic contains at least one of amphotericin B and nystatin. It is characterized by doing.

Further, the mouthwash according to claim 5 of the present invention is the same as the mouthwash according to claim 3 or 4, which contains at least 0.04 μg / ml of the polyene macrolide antifungal antibiotic. It is characterized by doing.

Further, the mouthwash according to claim 6 of the present invention is characterized in that, in the invention according to any one of claims 1 to 5, it contains a non-pathogenic bacterium or fungus. To do.

Further, the mouthwash according to claim 7 of the present invention is characterized in that, in the invention according to claim 6, the nonpathogenic bacterium is a lactic acid bacterium or a lactic acid bacterium.

The mouthwash of the present invention is contained in the oral cavity and is used for rinsing the range from the oral cavity to the tonsils. Further, the mouthwash of the present invention can be used as a mouth rinse by rinsing the mouth by containing it in the mouth.

The antifungal substance used in the mouthwash of the present invention may be any substance which can be used as a mouthwash to kill and reduce fungi such as Candida spp. The antifungal substance is limited to a specific antifungal substance. It is not something that will be done. As the antifungal substance, for example, an antifungal antibiotic substance is preferably used. As the antifungal antibiotic, conventionally known antifungal agents such as polyene-based, imidazole-based, triazole-based, and allylamine-based can be used.

These antifungal antibiotics are known to have serious side effects such as nephrotoxicity. In the present invention, however, the antifungal antibiotics are used as mouthwashes, and they are vomited after use, so that they can be used for a long period of time. There is almost no risk of side effects even when used as a mouthwash, and when used as a mouthwash, it hardly develops resistant fungi. Moreover, by reducing or sterilizing fungi such as Candida spp. In the oral cavity by gargling, in addition to recovering from oral candidiasis, invasion of fungi belonging to the genus Candida into the digestive organs from the oral cavity to the anus can be prevented. It can prevent and restore the biological function of digestive organs from the oral cavity to the anus, and normalize abnormalities of other organs. Further, since it is used as a mouthwash and does not swallow the antifungal agent as in the conventional case, it is effective even when the immunity or resistance is lowered.

Among the antifungal antibiotics, polyene macrolide antifungal antibiotics belonging to the polyene series are preferably used. As polyene macrolide antifungal antibiotics, for example, amphotericin B and nystatin are preferable, and these may be used alone or in combination. When antifungal antibiotics such as amphotericin B and nystatin are used as a mouthwash, it is sufficient that these antifungal antibiotics are contained in an amount of at least 0.04 μg / ml.
The concentration is not limited to a specific concentration as long as the concentration exceeds g / ml. 0.04 of such antifungal antibiotics
If the concentration is less than μg / ml, the expected effect cannot be expected as the active ingredient of the mouthwash. Of the polyene macrolide antifungal antibiotics, amphotericin B is preferable.

As amphotericin B, for example, fungizone syrup (trade name) marketed by Bristol-Myers Squibb Company, horizon syrup marketed by Fuji Pharmaceutical Co., Ltd. and the like can be used.
This fungizone syrup is a solution containing 100 mg amphotericin B in 1 ml.

Examples of the imidazole antifungal antibiotics include miconazole and econazole, and examples of the triazole antifungal antibiotics include fluconazole, itraconazole and fluconazole, and allylamine antifungals. Examples of fungal antibiotics include flucytosine and butenafine.

The mouthwash of the present invention preferably contains a non-pathogenic bacterium or fungus in addition to the antifungal agent. By containing these non-pathogenic bacteria or fungi, allelopathies between microorganisms are used to replace fungi and bacteria in the oral cavity such as Candida spp. With benign non-pathogenic bacteria and harmful Candida. It is possible to reduce fungi such as the amount of bacteria and further enhance the drug efficacy of the antifungal substance. Lactic acid bacteria are preferred as non-pathogenic bacteria, and harmless yeasts are preferred as non-pathogenic fungi. In addition, it is possible to make the mouthwash easier to use by adding various scent components as additives as mouthwash.

Examples of the lactic acid bacterium include Streptococcus genus, Leuconostoc genus, Pediococcus genus, Lactobacillus genus and Bifidobacteria genus. The lactic acid bacterium may be a living bacterium, a dead bacterium, or one obtained by extracting an active ingredient from the bacterium, and it is preferable to mix and use at least three bacterium species. In particular, lactic acid bacteria having a strong antibacterial property against Candida and Streptococcus muutans are preferable.

The above-mentioned antifungal antibiotics are diluted with various diluents and used as a mouthwash. As the diluting liquid, for example, purified water, a commercially available mouthwash, etc. can be used. Furthermore, other additives such as a bactericidal agent, a sweetener, a flavor, a coloring agent, a thickener, and a flavoring agent effective against fungi other than fungi can be added to the diluted solution. Examples of mouth rinses include mouth rinses containing sterilizing agents such as Listerine (trade name: manufactured by Warner Lambert), flavors, sweeteners, colorants and other components in an ethanolic solvent, and Mondamine (Earth Pharmaceutical Co., Ltd.). Commercially available mouth rinses such as manufactured by: trade name) can be preferably used. In addition, listerine is thymol, 1,
Components such as 8-cineol, 1-menthol, and methyl salicylate are dissolved in an aqueous solution of ethanol having a concentration of about 18%.

[0024]

BEST MODE FOR CARRYING OUT THE INVENTION In this embodiment, for example, 0.5 cc of fungizone syrup (trade name: manufactured by Bristol-Myers Squibb) is sampled with a pipette and added to 30 cc of listerine (trade name: manufactured by Warner Lambert). And mixed well to prepare a mouthwash. The concentration of amphotericin B in this mouthwash was 1.64 mg / ml.
Is. Others, thymol, 1,8-cineole, l-
It contains additives such as menthol and methyl salicylate. This mouthwash was applied to a patient (subject) undergoing dental treatment described below, and the Candida fungus in the oral cavity was observed before and after the mouthwash was applied.

[0025]

[Test Example] Saliva, tongue coating, upper anterior teeth, lower anterior teeth, upper molars and lower molars of 255 subjects were rubbed with a cotton swab to sample Candida spp. Candida spp. Culture was carried out in a culture medium. When scraping with a cotton swab, two cotton swabs were used for each site in order to sufficiently collect Candida.

In this test example, as a medium for Candida, Sabouraud agar medium (hereinafter referred to as "Sabouraud medium") (manufactured by Nissui), Chromoagar Candida slope agar medium (hereinafter
It is called "slope medium". ) (Manufactured by Kanto Kagaku), Chromogar /
Potato dextrose agar fractionation medium (hereinafter "fractionation medium"
Called. ) (Manufactured by Kanto Kagaku), Chromoagar agar medium (hereinafter referred to as “Chlomogar medium”) (Kanto Kagaku) and Candida yellow medium F (hereinafter referred to as “Yellow medium”) (manufactured by Fuji Pharmaceutical Co., Ltd.). Candida spp. Were cultivated depending on the application. At this time, many fungi of the genus Candida can be presumed and identified from the color tone of the colony.

The Sabouraud medium was divided into 6 parts, saliva, tongue coating, upper anterior teeth, lower anterior teeth, upper molars and lower molars, in order to find out where many Candida in the oral cavity at the time of initial examination. The sample at each of these sites was used and divided into respective divided portions for smear culture. This makes it possible to identify sites where many Candida bacteria are present. As a result, it was found that a large amount of Candida was found in the tongue coating and plaque in the interdental part of the molars. The slope medium and the chromoagar medium were used for the identification of Candida spp. The yellow medium was used for estimating the bacterial amount. As a result, Table 1 below
We were able to identify Candida strains that were classified into. Fractionated medium and chromoagar medium were used to judge whether Candida genus is present in the yellow medium that turned yellow when it seems that no decrease in the amount of Candida can be seen even after 2 to 3 months of treatment. .

As a result of culturing and separating the Candida spp. In all the test subjects as described above and identifying the Candida spp., The Candida spp.

[0029]

[Table 1]

These subjects were gargled once a day for 30 seconds to 1 minute with the mouthwash of this embodiment, and then gargled with purified water. This was continued for 3 months. After that, fungi such as Candida were detected in these subjects, but no fungus could be detected in any of the subjects.

Further, a dental examination and a medical inquiry were conducted on these test subjects. As a result, hemorrhoids were completely cured by 5 people,
Constipation was completely cured in 22 people, stool odor decreased in 36 people, arrhythmia normalized in 2 people, cholesterol level improved in 1 person, blood sugar level decreased in 3
In addition, findings such as improvement in prostate hypertrophy and 3 patients were observed, and none of the subjects had these symptoms but did not improve. In addition to these symptoms, findings such as healing of periodontal disease, improvement of halitosis, decreased frequency of stomatitis, and decreased pain after dental minor surgery were obtained. Especially, the improvement of diseases of the digestive system was remarkable, but the results of reduction of cholesterol level and blood glucose level and improvement of prostatic hypertrophy were more than expected.

As described above, according to this embodiment,
Not only is it effective against diseases caused by fungi mainly Candida in the oral cavity, but by reducing or sterilizing Candida inoculum in the oral cavity, Candida from the oral cavity to the digestive tract, etc. Can inhibit or prevent the invasion of fungi of the plant, and prevent the functional decline of the digestive tract caused by a large amount of toxins produced by Candida fungi, enhance the systemic immune function and restore the natural healing power. be able to. More specifically, the following drug effects were observed. Healing effect for gingivitis and periodontitis in the oral cavity, and prevention and healing effect for diabetes, heart disease, preterm birth, kidney disease, etc., which are suspected to be related to periodontitis. Bad breath, cheilitis, tongue pain. Effect of improving inflammation and rhinitis Healing and preventing effects of various inflammations from the digestive tract to the anus Preventive and healing effects of hemorrhoids Anti-tumor effect of digestive tract mucosa Reduced absorption of undigested protein from the intestinal tract and suppression of food allergy Efficacy, normalization of immune function by reducing stimulation by fungi and pathogenic bacteria in tonsils, and prevention and healing of immune diseases such as atopy and rheumatism Improvement of hypertension Improvement of intestinal function Palm-pus pus And curative effect of diseases caused by microbial products in the oral cavity and otolaryngology

The present invention is not limited to the above-mentioned embodiments, and any gargling agent containing an antifungal substance as an active ingredient is included in the present invention. In addition, the antifungal substance may be used by blending it with a dentifrice, or may be used in the form of gel in a mouthpiece.

[0034]

EFFECTS OF THE INVENTION According to the inventions of claims 1 to 7, the number of fungi and pathogenic bacteria such as Candida species in the digestive tract from the oral cavity to the throat and further to the anus is reduced. However, not only healing of oral diseases such as periodontal disease, but also healing of the entire digestive tract, immunity or resistance are improved, and recovery of various systemic diseases caused by fungi such as Candida spp. It is possible to provide a mouthwash capable of realizing the normalization of

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Claims (7)

[Claims] 1. A gargle comprising an antifungal substance as an active ingredient. 2. A gargle characterized by containing an antifungal antibiotic as an active ingredient. 3. The gargle according to claim 2, wherein the antifungal antibiotic is a polyene macrolide antifungal antibiotic. 4. The mouthwash according to claim 3, wherein the polyene macrolide antifungal antibiotic contains at least one of amphotericin B and nystatin. 5. The mouthwash according to claim 3 or 4, which contains at least 0.04 μg / ml of the polyene macrolide antifungal antibiotic. 6. The mouthwash according to any one of claims 1 to 5, which contains non-pathogenic bacteria or fungi. 7. The gargle according to claim 6, wherein the non-pathogenic bacterium is a lactic acid bacterium or a lactic acid bacterium.