JP2003329678A - Test substrate for biochemical test and solid-phase carrier - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a test substrate for use in biochemical tests which involve biochemical substances such as nucleic acid, nucleic acid derivatives, proteins, carbohydrates, cell fragments, etc. <P>SOLUTION: A splittable solid-phase carrier with a biochemical substance fixed to its fixation face is split to obtain the test substrate. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a test substrate for use in a biochemical test including a biochemical substance such as a nucleic acid, a nucleic acid derivative and a protein, and a solid phase carrier for manufacturing the test substrate.

[0002]

2. Description of the Related Art In the field of gene manipulation technology, technological development for efficiently analyzing all gene functions of various organisms, slight difference in nucleotide polymorphism, protein diversity, and the like has been advanced. The representative DNA chip is
It is a microarray in which a large number of oligonucleotides or DNA molecules are aligned on a solid phase carrier such as a slide glass.
The biomaterial analysis technology using a DNA chip can be applied to biomolecules and chemical substances other than DNA such as proteins, and research and development of drug discovery research, development of disease diagnosis and prevention methods, energy and environmental problem countermeasures, etc. Is expected to provide a new means to.

The DNA chip technology has been embodied in
It started with the discovery that the base sequence of DNA can be determined by hybridization with an oligonucleotide or a DNA fragment. DNA chip and microarray fabrication technology has been developed, and it has become possible to efficiently investigate mutations, polymorphisms, etc. of (many) gene expressions in a short time. A DNA chip is an element in which a large number of oligonucleotides or DNA fragments (also referred to as probes) corresponding to various genes are aligned and immobilized on a solid phase surface, and a large number of immobilized probes are formed on a matrix. Normal,
Thousands to tens of thousands of oligonucleotides were immobilized on one DNA chip. In the method using a DNA chip, mRNA as a sample is used by using a replication chain reaction.
(Messenger RNA) cDNA (complementary DN
A) is synthesized and fragmented, and then each fragment is fluorescently labeled to give a labeled fragment. These labeled fragments are brought into contact with a DNA chip and hybridized with the immobilized oligonucleotide or DNA fragment. The labeled fragment is an oligonucleotide or DNA having a complementary base sequence.
The excess amount of labeled fragment retained on the fragments is removed by a washing operation. Then, the amount and position of the retained labeled fragment are detected by fluorescence measurement, and the type of the corresponding oligonucleotide or DNA fragment is examined. This method is suitable for monitoring the expression of genes of known sequence.

It is also possible to label a fragment of genomic DNA instead of cDNA to similarly examine the homology with the probe.

The DNA chip manufacturing methods are roughly classified into the following three types. In the first method, a plurality of linkers modified with protective groups that can be removed photochemically are bound to the surface of the solid phase via amino groups and arranged. The photolithography technology was developed in the semiconductor manufacturing technology, and by applying this technology, the protective groups are removed by light irradiation through a mask that can irradiate only the desired linker fixing position. Next, a monomer having a protective group that can be removed photochemically is introduced to carry out the first coupling reaction only at the deprotected site. As a result, the oligonucleotide is extended only in that portion. By repeating the photolithography and the introduction of the monomer, a desired oligonucleotide matrix is formed.

Separately from this, there is also a method in which mononucleotide derivatives are successively sprayed onto a target site by an ink jet method or the like to be polymerized to synthesize a different oligonucleotide or the like at each site.

In the second method, a small amount of an oligonucleotide or a DNA fragment to be immobilized, which has been prepared in advance, is dropped onto the surface of a solid phase such as glass or a polymer film, and immobilized at that position by covalent bonding. For example, if an isothiocyanate group is introduced on the surface of the solid phase and the end of the oligonucleotide is an amino group, the oligonucleotide can be easily immobilized at the isothiocyanate group fixing position by covalent bonding.

In the third method, an oligonucleotide or a DNA fragment to be immobilized is prepared in advance, a small amount of it is dropped on the surface of a solid phase such as glass or a polymer film, and immobilized at the dropping position by adsorption or the like.

[0009]

The DNA chip prepared by the above method has a large number of probes immobilized on the same substrate, and is very useful in the field of research such as gene analysis. There are many combinations at the time of testing depending on the symptoms and the purpose of testing, and it is necessary to prepare a DNA sequence suitable for each, but this requires a lot of manufacturing costs, and thus thousands to several A general-purpose DNA identification substrate on which various oligonucleotides are immobilized is used. However, since complementary binding is performed for such a wide variety of DNAs, it is necessary to give a large number of replicate cultures of the DNA to be tested, and to each of them, an identification functional group such as a fluorophore is added. As a result, there is a problem that the amount of complementary binding of the test DNA becomes small, the complementary speed becomes slow, it takes a long time to obtain the result, and the cost is high.

Further, in the substrate type chip as described above, a method of statistically determining the result by reading the reaction at a large number of fixed points by coordinates is employed, and the analysis of the data also requires an inspection cost. It is a cause of increasing. Further, the DNA test is a matter related to the privacy of the individual, and there is no answer that it is necessary to perform only the test required by the individual.

Unlike the substrate type chip in which a large number of DNAs are fixed as described above, a bead type chip is also used. One kind of DNA is immobilized on one bead, and it is possible to collect and use the beads on which DNA necessary for the intended test is immobilized. However, it is difficult to give an identification code to each bead based on its shape, and it is difficult to identify the bead by coloring the bead or changing the size or the shape. Therefore, at the time of inspection, determination is made by a statistical method based on the color distribution as an aggregate and the size and shape distribution read by a laser.

According to the present invention, a test substrate for testing biochemical substances such as nucleic acids, nucleic acid derivatives and proteins can be miniaturized to reduce the amount of the substance to be inspected, and the reaction result can be obtained without using a statistical method. An inspection board that can be directly determined is provided.

[0013]

The test substrate of the present invention is a test substrate for performing a biochemical test using a biochemical substance, which is characterized in that the test substrate has a surface on which the biochemical substance is fixed. It is obtained by partitioning from a resolvable solid-phase carrier fixed to the.

Further, the present invention is characterized in that the biochemical substance fixing surface is divided by a groove, and only one kind of biochemical substance is fixed to each of the divided fixing surfaces. Furthermore, it is characterized in that it is a strip-shaped inspection substrate obtained by dividing in this way.

Furthermore, the present invention features a solid-phase carrier having a groove for fixing a biochemical substance on a fixing surface and dividing the substrate into a plurality of test substrates.

Here, the biochemical substances include nucleic acids, nucleic acid derivatives, proteins and sugars. Further, biochemical substances include cells, cell fragments, and cell constituent substances.

The test substrate of the present invention is prepared in advance with a dividable solid phase carrier having such a biochemical substance fixed on its surface, and the necessary biochemical substance is fixed according to the demand of the biochemical examination. Only a part is selected, divided, taken out, and provided for inspection, and it becomes possible to perform only the necessary inspection quickly and at low cost. The form of the inspection substrate obtained by dividing is preferably a strip shape in terms of handling, although it depends on the order of the fixed chemical substances.

Here, the biochemical test includes all tests, tests, experiments, and other operations using the above-mentioned biochemical substances. For biochemical tests, nucleic acids, nucleic acid derivatives,
It includes a process of detecting and fractionating these chemical substances using proteins, carbohydrates, cells, cell fragments or chemical substances. For example, such tests will provide new tools for simultaneous analysis of gene expression, mutation, polymorphism, etc., and drug discovery research, development of disease diagnosis and prevention methods, research and development of energy and environmental problem countermeasures, etc. The present invention relates to a substrate used for detecting and quantifying nucleic acids, nucleic acid derivatives, proteins, carbohydrates, cells and chemical substances which are highly expected to be provided.

In the present invention, it is preferable that a mark for identifying the fixed substance, that is, a code for identification is attached to the back surface of the substrate on which the biochemical substance is fixed. Such an identification code is written in advance on the solid-phase carrier, and in the subsequent fixing operation of the biochemical substance, division operation, and the necessary inspection process, while reading, it is possible to prevent erroneous operation under computer control. As a result, it is possible to efficiently carry out processing by an automatic machine.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION The test substrate of the present invention is obtained by dividing it from a solid phase carrier on which a biochemical substance is fixed in advance.
The solid phase carrier on which the biochemical substance is fixed is preferably provided with a dividing groove for easy division, and the biochemical substance is fixed on the fixing surface divided by the dividing groove. The dividing grooves of the solid phase carrier are provided in a grid pattern in the vertical and horizontal directions, and one direction is used as necessary.

These dividing grooves are formed in the step of producing a solid phase carrier. Further, the groove may be cut with a laser when the solid phase carrier is completed. Further, it is preferable that the fixing surface is a smooth flat surface in order to obtain accuracy in the fixing and testing steps of the biochemical substance.

On the fixed surfaces divided by the dividing grooves, different specific biochemical substances are fixed and stored as solid phase carriers. At the time of inspection, only those portions of these biochemical substances to which substances necessary for inspection purposes are fixed are divided from the solid-phase carrier and provided as inspection substrates.

For example, in the solid-phase carrier, different biochemical substances are fixed in the row direction on each fixed surface of the substrate divided into 100 vertical and horizontal dividing grooves, and the biochemical substance necessary for a certain test is fixed. If it is contained in 10 to 40 lines, this substrate can be divided into columns and used as strip-shaped inspection substrates. To improve accuracy,
It is also possible to use a sequence of numbers together. Further, it is also possible to divide only the portion of the 10th to 40th rows and use only the object related to the target inspection after the column division.

By using such a method, it is possible to reduce the amount of extra biochemical substance used for purposes other than the purpose of inspection, thereby enabling rapid inspection and reducing inspection costs. In addition, from the viewpoint of protecting privacy, unnecessary information other than the purpose of inspection will be cut off.

As an example of the inspection board, FIG. 1 shows the board divided into one row.

The inspection board 1 is in the form of a strip that is divided into one row, and the biochemical substance 6 is fixed to the fixing surface 2a that is separated by the plurality of dividing grooves 5. With such a strip shape, the biochemical substances 6 necessary for the inspection become the inspection substrate 2 arranged in a line, the handleability is good, and the inspection time is shortened. This inspection substrate 1 is obtained by dividing a solid phase carrier 2 having a plurality of dividing grooves 5 as shown in FIG. 2A into one row.

The biochemical substance fixing surface 2a of the solid phase carrier 2 which can be divided is preferably a smooth surface having a surface roughness Ra of 0.5 μm or less. This surface roughness is particularly preferable for the inspection substrate 1 in which the fluorescence determination is performed by utilizing the fluorescent label of the biochemical substance 6 in the inspection process. That is, the surface roughness Ra
When the surface roughness is more than 0.5 μm, when the inspection is performed by fluorescence in the inspection process, the excitation light for fluorescent color is scattered on the surface, which makes it difficult to determine the fluorescence. Further, the flatness of the fixed surface 2a improves the inspection accuracy. In particular, a smooth surface having a surface roughness Ra of 0.2 μm or less is preferable.

The solid phase carrier 2 can be formed of polymer, ceramics, metallic silicon or the like. It is particularly preferred that these materials are hydrophobic or poorly hydrophilic.

As examples of polymers, cellulose acetate-based, polyvinyl-based, polyester-based polymers and the like can be used. Examples of polyvinyl polymers include polystyrene and polymethylmethacrylate. Polyester-based polymers include, for example, polyethylene terephthalate and bisphenol A polycarbonate.

The ceramic includes an oxide sintered body,
For example, alumina, silica, zirconia, titania, mullite, sapphire, etc. can be used, and further, silicon carbide, which is a non-oxide ceramic, etc., can also be used. Ceramics include glass, and for example, silicate glass and borosilicate glass can be used. Ceramics whose surface is coated with a glass layer are also preferably used.

The material of the solid phase carrier includes a metal, particularly a metal or a submetal having an inert property and a high corrosion resistance, and an alloy thereof. For example, silicon or other semiconductor material is used.

Among these, glass, silicon and ceramics are particularly used, and these materials have an advantage that the surface treatment for smoothing as described above is easy and the analysis by the fluorescence scanning device is easy.

The solid phase carrier 2 has a flat surface on the fixing surface 2a made of such a material, and its shape is not particularly limited, but a square plate-like thin plate can be usually used. As for the external dimensions, for example, those having a length and width of 50 to 60 mm and a thickness of 0.1 to 2 mm can be used.

The solid phase carrier 2 is provided with a dividing groove 5. As a manufacturing method for adding the groove 5, in the step of forming the square plate-shaped thin plate, a lattice-shaped split groove is provided on the surface thereof. Alternatively, a method of cutting a split groove with a laser after manufacturing a square plate-shaped thin plate is used. The case of ceramics will be described as an example.

The thin plate-shaped alumina ceramic substrate is generally produced by a tape method. The alumina raw material and the sintering aid powder are crushed and mixed by a ball mill using a solvent, and the organic binder is further dissolved to obtain mud. After the defoaming treatment, a doctor blade method is used to flow mud on the release paper, the solvent is blown off with warm air, and after drying, the release tape is peeled off to produce a raw tape of uniform thickness. A mold having teeth for grooves is pressed against this green tape to add grooves to the tape surface. At the same time, the raw tape is punched out with a die having a predetermined size.

The grooved green tape thus obtained is fired at a predetermined temperature to obtain a ceramic substrate having divided grooves. Further, it is also possible to add a groove to the raw tape by firing without punching it into a predetermined size and then add a dividing groove with a laser.

The division by the dividing groove can be preferably applied to relatively brittle materials such as silica glass, sapphire, silicon and ceramics.

The test substrate 1 is obtained by fixing the biochemical substance 6 on the solid-phase carrier 2 having the above-mentioned dividing groove 5 and then dividing it. Before the operation of immobilizing the biochemical substance 6, the solid-phase carrier 2 is subjected to the additional description of the identification symbol 3 described later and the surface treatment necessary for immobilizing the biochemical substance.

The divisible solid phase carrier 2 preferably includes a surface layer 4 formed in advance on the immobilizing surface 2a of the solid phase carrier according to the biochemical substance 6 to be immobilized. FIG. 2C shows an example in which a surface layer 4 is formed on the entire surface of the divisible solid phase carrier 2. The surface layer 4 is used as a binding layer for securely fixing the biochemical substance 6 on the fixing surface of the solid phase carrier 2. When the material of the solid phase carrier 2 is hydrophobic or poorly hydrophilic, the surface layer 4 is a nucleic acid,
It binds to substances to be immobilized such as nucleic acid derivatives, proteins, sugars, cells, cell fragments or other chemical substances by electrostatic bonding or covalent bonding to ensure immobilization on a solid phase carrier. .

The surface layer 4 on the fixed surface is made of gold or poly-L-
Thin films of polycationic substances such as lysine and polyethyleneimine can be used. The solid phase carrier 2 can be surface-treated to form a thin film. The thin film can also be formed by surface-treating it with a silane coupling agent having an alkyl group such as an amino group, an aldehyde group and an epoxy group. The treatment with poly-L-lysine or the like may be combined with the treatment with a silane coupling agent.

A surface layer 4 may be further formed on the fixed surface 2a or the thin film formed on the fixed surface 2a. Such a surface layer 4 includes a layer containing a hydrophilic polymer substance having a charge and a layer formed of a crosslinking agent.

The solid-phase carrier 2 of this embodiment is provided with an identification code 3 in advance. The identification code 3 indicates the biochemical substance 6 fixed on the surface of the inspection board, and by detecting the identification code 3 and collating it with the data to decode it,
It identifies the fixed substance.

As the identification code 3, a known code can be used, and it is marked on the bottom surface of the substrate to provide characters, numbers, symbols, other codes, or optically or magnetically readable code information, for example, two-dimensional. Target, three-dimensional bar code, or dot code. In particular, the fixed surface 2 surrounded by the dividing groove 5
Since a is small, it is preferable that the identification code 3 has a fine shape and can be detected optically or magnetically. FIG. 2B shows an example in which the identification code 3 composed of a two-dimensional code is formed on the bottom surface 2b of the solid phase carrier 2.

The identification code 3 can be displayed by printing characters, numbers, symbols or the above bar code with ink or paint. The ink or paint used in this case is preferably selected from paint materials having chemical resistance and corrosion resistance to the solvent that dissolves or suspends the biochemical substance sample in the inspection step. Preferably, a UV-curable resin-containing ink may be applied as a coating film, and an UV pattern representing an identification code may be irradiated, or laser scanning may be performed for printing.

In addition to printing with ink or the like, the identification code may be directly detected on the bottom surface of the substrate by laser irradiation as an optically detectable one. Figure 2
In the example of (B), an array of patterned dot codes is engraved on the bottom surface 2b of the solid-phase carrier 2 with a laser beam.

Further, as the magnetically detectable identification code, a code in which a magnetic layer is formed on a substrate and magnetized by a magnetic head to be symbolized so as to be readable can be employed.

Identification code 3 displayed by laser irradiation
Is advantageous because it is possible to partially melt the surface of the solid support by the heat of the scanning laser beam on the irradiation surface and engrave and draw the required marks, because chemical resistance does not have to be taken into consideration.

On the other hand, in the case of the identification code by laser irradiation, there is a phenomenon that the edge portion of the engraved code rises. In FIG. 2 (B), around the engraved dot code, that is, the edge portion is annularly raised. This rise is preferably 5 μm or less, and more preferably 1 μm or less. When the edge portion has a height of 5 μm or more, the solid phase carrier 2 can be stably placed on the work table when the biochemical substance 6 to be described later is placed on the solid phase carrier 2 on which the identification code 3 is engraved. This is because the substrate cannot be placed and the substrate may be cracked along the dividing groove 5.

FIG. 2D schematically shows the dividable solid phase carrier 2 having the biochemical substance 6 immobilized on the surface layer 4 on the immobilizing surface 2a of the solid phase carrier 2. Various methods are used for immobilization, and there are methods such as the Affymetrix method of synthesizing an oligonucleotide having an arbitrary base sequence using photolithographic technology and the method of dripping and fixing a preliminarily prepared biochemical substance on a substrate. is there. In the latter spotting method, the liquid containing the biochemical substance 6 is carried on the tip of the pin and the droplet is spotted on the surface of the solid phase carrier. There is a method using a stamp pin such as the & ring method or a method using an inkjet method.

Prior to the immobilization on the solid phase carrier 2, the biochemical substance 6 is treated so that its molecular ends are electrostatically or covalently bonded to the thin film or the surface layer 4 on the substrate surface.
It is preferable to previously introduce a functional group capable of participating in the interaction. Such functional groups can include thionyle groups, amino groups, aldehyde groups, carboxylic acid groups, biotin. In particular, the functional group is preferably a thionyl group or an amino group.

As shown in FIG. 2D, the solid-phase carrier 2 immobilized and adjusted as described above has the above-mentioned biochemical substance 6 such as nucleic acid on the surface of the fixed surface 2a surrounded by the dividing groove 5. Is fixed. Further, the bottom surface 2b is additionally provided with an identification code 3 for identifying the immobilized biochemical substance 6. Depending on the purpose and specifications of inspections, tests, experiments, etc., only the portion where the required specific type and amount of biochemical substance 6 is fixed is divided from this solid-phase carrier 2 and collected for use. It The solid phase carrier 2 is divided by applying a bending stress to the dividing groove 5 and is divided by an automatic dividing machine while discriminating the identification code 3 on the bottom surface with a laser.

For each row of the solid-phase carrier 2 provided with grooves 5 for dividing in a grid pattern, a biochemical substance 6 necessary for a certain inspection purpose is provided.
Is fixed, it is divided into columns according to the contents of the inspection and used as the strip-shaped inspection substrate 1.

According to this method, only necessary tests can be conducted, consumption of expensive biochemical substances can be reduced, and inspection time can be shortened to reduce inspection cost. In addition, since there is no information other than the purpose of inspection, it is useful for protecting privacy.

[0054]

EXAMPLES Example 1 In this example, a solid phase carrier was prepared by selecting alumina ceramics as a substrate material. The alumina flat plate has an outer shape with dimensions of 60 × 50 mm and a thickness of 0.15 mm, and the front and back surfaces have a space of 0.6 mm in length and 0.35 in width.
A large number of dividing grooves which are orthogonal to each other are formed at mm intervals. The division grooves were formed by engraving the grooves on a raw alumina tape with a die press and then firing.

The surface roughness Ra was 0.2 μm, 0.35 μm, and 0.5 μ by sandblasting an alumina flat plate.
Alumina flat plates of m and 0.6 μm were obtained.

With respect to this alumina flat plate, using a fluorescence spectrophotometer at excitation wavelengths of 532 nm and 630 nm,
The fluorescence spectrum of each wavelength was measured by irradiating with light having a band width of 1.5 nm. With respect to an alumina flat plate having a surface roughness Ra of 0.2 nm, absorption maximum peaks were not observed at 570 and 660 nm which are detection wavelengths of Cy3 and Cy5 which are often used as fluorescent labels in DNA inspection. It has been found that it can be used as a substrate for inspection using fluorescent labels. When the surface roughness Ra increases, the bandwidth of the reflected light of the excitation wavelength tends to widen, and when the surface roughness Ra becomes 0.6 nm, the reflected light bandwidth spreads to the fluorescence detection wavelength range and is emitted from the sample. It has been found that the accuracy of the inspection is deteriorated because the fluorescence wavelength light generated overlaps with the light reflected from the substrate.

An identification code was engraved on the bottom surface of the alumina plate having the dividing grooves by scanning with a laser beam. 0.55 × 0.25 inside the area defined by the dividing groove
In consideration of thousands of kinds of biochemical substances and other types of test bodies, a YAG laser beam was scanned and a two-dimensional barcode was engraved in each area of mm area. Check the laser reading of the dug up two-dimensional bar code,
It was readable within seconds and was effective as an identification code.

The surface on which the identification code was engraved was observed using a surface shape microscope, and the amount of swelling or height of the edge portion of the spot hole formed on the surface was measured by irradiating a laser beam. The laser irradiation output was reduced and the digging depth readable by the image sensor was measured.

The swelling of the edge portion around the beam spot hole was 0.8 μm.

The laser irradiation output was increased stepwise for each alumina substrate, and substrates having different swells around the beam spot holes were produced. When this board was set in a device that spots biochemical substances and the device was operated,
If the swelling around the beam spot hole is 5 μm or more, cracks may occur along the dividing groove, and the swelling around the beam spot hole should be 5 μm or less to stably stamp the biochemical substance. I found it necessary.

Example 2 Based on the findings of Example 1,
After engraving an identification code with a YAG laser on the back surface of an alumina flat plate having a surface roughness Ra of 0.35 μm, the following treatment was performed.

The alumina plate was immersed in a mixed solution of a 20% aqueous sodium hydroxide solution and 95% ethanol, washed at room temperature for 2 hours, and then washed with distilled water.

In order to form a surface layer on an alumina flat plate,
It was immersed in a 2% ethanol solution of aminopropyltrimethosisilane, stirred for 1 hour at room temperature with stirring, and then taken out and washed with ethanol. Then, in the dryer 1
It was heated and dried at 10 ° C. for 15 minutes to obtain a solid-phase carrier coated with a silano compound thin film.

After the active esterification of this solid-phase carrier, oligo nucleotides having different base sequences were used on the fixed surface divided into the dividing grooves, and the pin on the bottom was used while reading the identification code on the bottom with an image sensor. I put it on.
By this operation, a solid-phase carrier on which the oligonucleotide was fixed was obtained.

This solid phase carrier has a base sequence of 20 to 7
Two 0 oligonucleotides are spotted in the short side direction (row) of the plate, and they are arranged in the same manner as each row (the long side direction (row) is an oligonucleotide having the same base sequence as the neighbor. Exist).

By dividing this solid phase carrier into columns by using a substrate divider, a width of 0.58 m having two spots of oligonucleotide having a base sequence of 20 to 70 is obtained.
It was possible to obtain a strip-shaped inspection substrate having a length of m and a length of 40 mm.

A fluorescein-conjugated oligonucleotide complementary to the 25-nucleotide oligonucleotide was added to the test substrate for hybridization, and then the excess fluorescein-conjugated oligonucleotide was washed off with ethanol. After drying, fluorescence measurement was performed. In the above-mentioned test substrate on which oligonucleotides having nucleotide sequences 20 to 70 were spotted, clear fluorescence with a wavelength of 473 nm was observed at two spots on which oligonucleotides having 25 bases were spotted. Moreover, the identification code provided on the bottom surface was read by an image sensor, and it was possible to confirm that the spot where fluorescence was observed was an oligonucleotide having 25 bases.

The test substrate is obtained by dividing one row of the solid phase carrier, and the test substrate can be further divided along the dividing groove to take out only the oligonucleotide portion having the number of bases to be detected and used.

[0069]

INDUSTRIAL APPLICABILITY The test substrate of the present invention is a biochemical substance immobilized on a divisible solid phase carrier, such as a nucleic acid, a nucleic acid derivative, a protein, a carbohydrate, a cell or a cell fragment, which is necessary for testing. Since only the part where the biochemical substance is fixed can be obtained by selectively dividing it, the number of test substrates used to detect the biochemical substance is small, and the amount of the substance to be inspected is reduced and Since there is no manifestation other than the items described above, it is possible to reduce the inspection time and cost.

Further, by attaching the identification code of the biochemical substance fixed on the surface to the bottom surface of the separable solid phase carrier, the portion required for the inspection can be confirmed and the subsequent inspection steps can be performed successively by the inspection identification code. Can be read to identify the biochemical substance fixed on the inspection substrate, and the inspection process can be performed while avoiding erroneous operation.

Further, since there is no expression of information other than the purpose of inspection, it is useful for protection of privacy.

[Brief description of drawings]

FIG. 1 is a perspective view of an inspection board according to an embodiment of the present invention.

FIG. 2 shows a solid phase carrier of the present invention, (A) is a perspective view, and (B) to (D) are partial plan views.

[Explanation of symbols]

1 inspection board 2 Solid support 3 identification code 4 surface layer 5 grooves 6 biochemical substances

Claims (8)

[Claims] 1. A test substrate for performing a biochemical test using a biochemical substance, which is obtained by dividing a divisible solid phase carrier having a biochemical substance fixed on a fixing surface. Inspection board. 2. The test substrate according to claim 1, wherein the biochemical substance fixing surface is divided by grooves, and only one kind of biochemical substance is fixed to each of the divided fixing surfaces. 3. The biochemical substance fixing surface has a surface roughness Ra.
The inspection substrate according to claim 1, which has a thickness of 0.5 μm or less. 4. An identification code for identifying a biochemical substance to be fixed is attached to the bottom surface of the solid phase carrier.
The inspection board according to any one of 1 to 3. 5. The inspection board according to claim 4, wherein the solid phase carrier is made of ceramics, and the identification code is dug into the surface of the ceramics by laser beam irradiation. 6. The inspection board according to claim 5, wherein the protrusion of the edge portion of the identification code dug into the ceramic surface is 5 μm or less. 7. The inspection board according to claim 1, wherein the inspection board has a strip shape. 8. A solid-phase carrier comprising a groove for fixing a biochemical substance on a fixing surface and dividing the substrate into a plurality of test substrates.