JP2003315331A5 - - Google Patents
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- JP2003315331A5 JP2003315331A5 JP2002120616A JP2002120616A JP2003315331A5 JP 2003315331 A5 JP2003315331 A5 JP 2003315331A5 JP 2002120616 A JP2002120616 A JP 2002120616A JP 2002120616 A JP2002120616 A JP 2002120616A JP 2003315331 A5 JP2003315331 A5 JP 2003315331A5
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- JP
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- Prior art keywords
- keratinocytes
- present
- skin
- shape
- differentiation
- Prior art date
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- 210000002510 keratinocyte Anatomy 0.000 description 13
- 238000000034 method Methods 0.000 description 11
- 210000003491 skin Anatomy 0.000 description 9
- 230000004069 differentiation Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000004299 exfoliation Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Description
（２）本発明の角質細胞の鑑別法、皮膚特性の鑑別法
本発明の角質細胞の鑑別法は、前記本発明の角質細胞の形状の鑑別の為の映像を用いることを特徴とする。角質細胞の形状により、皮膚特性、例えば、肌の刺激に対する感受性、バリア機能を含む肌の状態、肌の老化の程度を表す肌年齢などを知ることができる。この様な情報を得るための角質細胞の形状としては、角質細胞の配列の規則性、大きさ（体積或いはその便宜的代替値としての面積）、一層で剥離してくるか、数層が重なったまま剥離してくるかという、角質細胞の剥離のパターン等が例示できる。本発明の映像を用いることにより、これらの何れもが、角質細胞標本を染色して得た画像と同程度の精度で鑑別、測定することができる。配列規則性であれば、個々の細胞の形がどの程度六角形になっているかを肉眼で判定したりすることにより鑑別できるし、大きさであれば、二値化などの処理を施した後に、画像処理ソフトを使用することにより自動計測することができる。剥離パターンは、配列規則性同様に、肉眼判定できるし、或いは、画像の濃さ（或いは明るさ）などを、輝度から算出し、推定することもできる。この様な方法については、染色標本において使用されている技術をそのまま応用することができる。又、この様に鑑別された角質細胞の形状をもとに、従来知られている角質細胞と皮膚特性の関係を利用して皮膚特性を鑑別することもできる。かかる皮膚特性の鑑別が、本発明の皮膚特性の鑑別法である。この様な技術で、既に知られているものとしては、特開２００２−１７６８８号、特開２００１−１０８６７４号、特開２００１−１３１３８号、特開２０００−１１６６２３号、特開平１１−３０４７９８号、特開平１１−２９９７９２号、特開平７−２０９２９２号等が例示でき、これらの何れもが使用することができる。従来の染色標本法が標本調整に、染色剤などを用いることができる環境において数時間要していたのに比べ、本発明の鑑別法は、通常の環境で、ほぼリアルタイムに鑑別を行うことができる。即ち、染色剤などを用いることができる環境への角質細胞の移送時間を加味すれば、従来法に比して、本発明の鑑別法では、数日間の時間短縮が行える。加えて、染色剤を必要としないことから廃水処理の必要性もなく、環境保全の観点からも本発明の鑑別法は好ましい。この様に、皮膚特性を鑑別することにより、適切な化粧料の選択が可能となり、誤った化粧料の選択による、トラブルの発生や無駄な投資を避けることも可能である。又、本発明の鑑別法は、採取した角質細胞には非侵襲的であるので、角質細胞の形状の鑑別を行った後に、種々の染色標本に供することも可能であり、従来の方法に比して、更に多面的な情報の入手も可能となる。
(2) Method of Differentiating Keratinocytes of the Present Invention, Method of Differentiating Skin Properties The method of differentiation of keratinocytes of the present invention is characterized by using an image for discrimination of the shape of the keratinocytes of the present invention. Depending on the shape of keratinocytes, it is possible to know skin characteristics such as sensitivity to skin irritation, skin conditions including barrier function, skin age indicating the degree of skin aging and the like. As the shape of keratinocytes for obtaining such information, regularity of the arrangement of keratinocytes, size (volume or area as its convenient alternative value), exfoliation in one layer or several layers overlap A pattern of exfoliation of keratinocytes can be exemplified as to whether exfoliation occurs while staying flat. By using the image of the present invention, any of these can be identified and measured with the same degree of accuracy as an image obtained by staining a keratinocyte sample. In the case of sequence regularity, discrimination can be made by visually determining how the shape of each cell is hexagonal, or if it is a size, after processing such as binarization, etc. Automatic measurement can be performed by using image processing software. The peeling pattern can be determined with the naked eye similarly to the arrangement regularity, or the density (or brightness) of the image can be calculated from the luminance and estimated. For such a method, the technique used in the stained sample can be applied as it is. In addition, based on the shape of the keratinocytes thus differentiated, it is also possible to distinguish skin characteristics by using the conventionally known relationship between keratinocytes and skin characteristics. Such differentiation of skin characteristics is the method of differentiation of skin characteristics of the present invention. Among such techniques, as already known, JP-A-2002-17688, JP-A-2001-108674, JP-A-2001-13138, JP-A-2000-116623, JP-A-11-304798, JP-A-11-299972, JP-A-7-209292 and the like can be exemplified, and any of these can be used. The conventional stained specimen methods specimen adjustment, compared to it takes several hours in an environment which can be used as the staining agent, differentiation method of the present invention, under normal circumstances, it is carried out differential in near real time it can. That is, if the transport time of keratinocytes to an environment where a staining agent etc. can be used is taken into consideration, the differentiation method of the present invention can shorten the time by several days as compared with the conventional method. In addition, the identification method of the present invention is preferable also from the viewpoint of environmental protection because there is no need for waste water treatment because it does not require a staining agent. In this way, by discriminating the skin characteristics, it is possible to select an appropriate cosmetic, and it is also possible to avoid the occurrence of troubles and unnecessary investment due to the incorrect cosmetic selection. In addition, since the discrimination method of the present invention is non-invasive for the collected keratinocytes, it is possible to differentiate the shape of the keratinocytes and then to use for various staining specimens, and it is possible to compare with conventional methods. Thus, it is possible to obtain more versatile information.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002120616A JP3709382B2 (en) | 2002-04-23 | 2002-04-23 | How to differentiate corneocytes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002120616A JP3709382B2 (en) | 2002-04-23 | 2002-04-23 | How to differentiate corneocytes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003315331A JP2003315331A (en) | 2003-11-06 |
| JP2003315331A5 true JP2003315331A5 (en) | 2005-04-28 |
| JP3709382B2 JP3709382B2 (en) | 2005-10-26 |
Family
ID=29536791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002120616A Expired - Lifetime JP3709382B2 (en) | 2002-04-23 | 2002-04-23 | How to differentiate corneocytes |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3709382B2 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9750449B2 (en) * | 2003-12-12 | 2017-09-05 | Johnson & Johnson Consumer Inc. | Method of assessing skin |
| JP2006017688A (en) * | 2004-06-01 | 2006-01-19 | Pola Chem Ind Inc | Differentiation method for subcorneal cell by autofluorescence |
| JP4549108B2 (en) * | 2004-06-11 | 2010-09-22 | ポーラ化成工業株式会社 | How to measure skin flexibility |
| JP4818704B2 (en) * | 2005-12-15 | 2011-11-16 | ポーラ化成工業株式会社 | Image color adjustment method |
| US8526689B2 (en) | 2007-02-14 | 2013-09-03 | Pola Chemical Industries Inc | Method of supporting the differentiation of corneocytes |
| CN102819732B (en) * | 2007-02-14 | 2016-02-10 | 宝丽化学工业有限公司 | Support method and the computing machine of resolution angle cell plastid |
| JP5065237B2 (en) * | 2008-12-26 | 2012-10-31 | 常盤薬品工業株式会社 | Noninvasive evaluation method of local pathology of atopic dermatitis |
-
2002
- 2002-04-23 JP JP2002120616A patent/JP3709382B2/en not_active Expired - Lifetime
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