JP2003252755A - Healing-effect composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a healing-effect composition directly having influence on electroencephalogram in an ordinarily edible food form and capable of bringing about healing state and a food containing the composition. <P>SOLUTION: The present invention relates to the GABA-containing healing- effect composition directly increasing appearance of brain alpha wave and decreasing appearance of beta wave and positively imparting healing feeling in which a ratio of appearance frequency of the beta wave to appearance frequency of alpha wave exhibits a value smaller than 1 by orally ingesting GABA and the food containing the composition. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a healing effect composition containing GABA. Further, it relates to a food or drink containing the above-mentioned healing effect composition.

[0002]

2. Description of the Related Art "Healing" is a very important factor in a stressful modern society.

In modern times, there are many factors that provide a "healing" effect. For example, music, pets, delicious meals, hot springs, and travel. However, the above-mentioned ones do not always exert a "healing" effect on all people, because the taste of each person is a major factor.

[0004] Generally, as an index showing human emotions, there is a method of expression by electroencephalogram.

A human brain wave has a frequency of 3.5 depending on its frequency.
Hz delta wave, 3.5 Hz to 7.5 Hz theta wave, 7.5 Hz to 13.5 Hz alpha wave and 1
There are beta waves above 3.5 Hz. Of these electroencephalograms, alpha waves increase during relaxation,
It is known to decrease when stress is applied.
On the other hand, beta waves are known to appear when the brain is awake and excited.

The appearance state of alpha waves is often used as an index of the degree of relaxation, and in recent stressful society, various attempts have been made to positively enhance alpha waves to relax them. However, no mention is made of the regulation and reduction of beta waves when excited.

It is known that GABA (gaba, γ-aminobutyric acid) is widely distributed in the living body, especially in the brain,
It is known as a suppressor neurotransmitter. But G
ABA is said not to cross the blood-brain barrier, so GABA taken orally does not normally affect GABA release in the central nervous system of the brain.

[0008]

SUMMARY OF THE INVENTION An object of the present invention is to provide a composition which directly affects the electroencephalogram in a food form which can be eaten normally.

A healing composition which can be taken on an as-needed basis on a daily basis, and which can bring about a "healing state" by effectively enhancing alpha waves and at the same time actively decreasing beta waves, and It is to provide a food containing the composition of the present invention.

[0010]

Means for Solving the Problems As a result of intensive studies by the present inventors, oral ingestion of GABA has the effect of increasing the appearance of cerebral alpha waves and further decreasing the appearance of beta waves. I found that. Although GABA is a neurotransmitter of the suppressive system, it has been reported that it generally does not cross the blood-brain barrier as described above, and it is unexpected that oral intake directly affects EEG. . The present invention has been completed based on this finding.

That is, the healing composition of the present invention is GAB.
It is characterized by containing A as an active ingredient.

Further, the healing composition of the present invention increases the number of appearances of alpha waves, which are brain waves, and at the same time reduces the number of appearances of beta waves, and the ratio of the number of appearances of beta waves / the number of appearances of alpha waves. Is characterized by exhibiting a healing effect of showing a value smaller than 1.

The healing composition of the present invention is GAB.
It is preferable to contain A in an amount of 5 to 70 mass%.

The food or drink of the present invention is characterized by containing the above-mentioned healing effect composition.

According to the present invention, oral ingestion directly increases the cerebral alpha wave appearance and positively gives a "healing sensation" produced by decreasing the beta wave appearance.
It is possible to provide a highly safe healing effect composition and foods and drinks containing the same.

[0016]

BEST MODE FOR CARRYING OUT THE INVENTION Preferred embodiments of the present invention will be described below.

GABA, which is an active ingredient of the healing composition of the present invention, refers to γ-aminobutyric acid, which is one of the amino acids contained in trace amounts in tea, vegetables, cereals and the like as a food ingredient.

In the present invention, a preferred method for producing GABA is a fermentation method using a lactic acid bacterium capable of obtaining GABA in large quantities and at low cost. For example, sodium glutamate,
Add lactic acid bacteria (Lactobacillus) to a culture solution containing glucose, baker's yeast extract, sodium acetate, magnesium sulfate, etc.
Brevis (IFO12005 strain), Lactobacillus hirugardi K-3 strain (FERM P-18422), etc.)
The culture solution is added, and the mixture is cultured at 20 to 30 ° C. for 1 to 3 days. The culture solution is sterilized by heating and then filtered to obtain a filtrate.
As lactic acid bacteria, Lactobacillus hirugardi K
When strain -3 (FERM P-18422) is used, the GABA content in the culture broth is about 5% by mass, and GABA accounts for about 70% in terms of solid content.

This filtrate can be used as the healing effect composition of the present invention by appropriately concentrating it as it is or further drying it as a powder. GABA can be further purified by subjecting the filtrate to a treatment such as column chromatography. For example, ion exchange resin D
By performing column chromatography using SR-01 (trade name, manufactured by Mitsubishi Chemical Corporation), more GA
A composition having a high BA content (80% by mass or more) can be obtained.

GABA in the healing composition of the present invention
The content of is not particularly limited, but is preferably 5 to 70 mass%, more preferably 20 to 70 mass%.

When the content of GABA in the healing effect composition is less than 5% by mass, it becomes difficult to add a sufficient healing effect when added to foods and drinks.
On the other hand, if it exceeds 70% by mass, purification is costly, which is not preferable.

The form of the healing composition of the present invention is not particularly limited, and examples thereof include granules, powders, tablets and liquids.

The effective intake of the healing composition of the present invention is
GABA usually varies depending on specific symptoms, age, etc.
In terms of conversion, 10 to 3000 mg / day is preferable, and 100 to 500 mg / day is more preferable.

The "healing" in the present invention means increasing the number of appearances of alpha waves and simultaneously decreasing the number of appearances of beta waves. At that time, (the number of times the beta wave appears)
The state where the ratio of / (number of appearances of alpha waves) is less than 1 is called “healing”, and the smaller the value of this ratio, the greater the healing effect.

When the healing effect composition of the present invention is added to foods and drinks, the addition amount can be appropriately set, but it is usually GA.
0.05-20 mass% in terms of BA is preferable, and 0.1
10 mass% is more preferable. If the amount of the healing effect composition added is out of the above range, a sufficient healing effect cannot be imparted or the flavor of the food or drink is affected.

The food and drink are not particularly limited,
Examples thereof include bread, cakes, confectionery (cookies, snacks, gums, chocolates, etc.), beverages (soft drinks, mineral water, favorite beverages, alcoholic beverages, etc.), and supplements (tablets, soft capsules, etc.).

[0027]

The present invention will be specifically described below with reference to examples, but the present invention is not limited thereto.

Example 1 Preparation of Lactobacillus fermented GABA Fermentation raw materials (pH 5.
0) After sterilizing 50 L by heating at 90 ° C. for 10 minutes, kimchi-derived lactic acid bacteria (Lactobacillus hirugardi K-3
Strain (FERM P-18422)) culture solution (50 mL) was inoculated and cultured at 30 ° C. for 3 days. The GABA content of the fermented liquid after the fermentation was 51 g / L. This fermentation broth was sterilized by heating at 90 ° C. for 10 minutes, then a filter aid was added and filtered, and the obtained filtrate was concentrated with a vacuum concentrator, dried with a freeze dryer, pulverized into powder. Lactic acid bacteria fermentation GA
A BA composition (GABA content: 65% by mass) was obtained.

[0029]

[Table 1]

As the GABA fermentation raw material, glutamic acid, wheat gluten, shochu lees protein, etc. may be used as a glutamic acid source in addition to sodium glutamate.

Test Example 1 GABA concentration measurement The GABA concentration was measured by the following method.

Each sample was appropriately diluted with a 0.2N sodium citrate buffer (pH 2.2), and then centrifuged or filtered to remove solids, and used as a measurement sample. The contents of GABA and glutamic acid were analyzed by a high performance liquid chromatograph under the following conditions according to the usual method for measuring the amino acid content. Equipment used: High-performance liquid chromatograph LC-9A manufactured by Shimadzu Corporation Analytical column: Strongly acidic cation exchange resin column Shin
-Pack Isc-07 Na type moving layer buffer: amino acid moving layer kit manufactured by Shimadzu Corporation Na type moving layer flow rate: 0.3 ml / min Reaction layer 1: 0.04% sodium hypochlorite solution (pH
10 ml of boric acid-carbonate buffer solution (500 ml) and hypochlorous acid (0.2 ml) Reaction layer 2: Amino acid analysis kit O manufactured by Shimadzu Corporation
Flow rate of PA reagent reaction layer: 0.2 ml / min Detection: Fluorescence detection Ex348 nm, Em450 nm

Example 2 Number of appearance of alpha wave and beta wave by ingestion of GABA The effect of alpha wave appearance by ingestion of the composition of the present invention obtained in Example 1 was examined. First, the test subject measured an electroencephalogram for 1 hour in a room (25 ° C, 40 lux) shielded from the outside. EEG multi-purpose telemeter (NEC)
Was used.

The measurement was carried out on 10 subjects, and the alpha wave for 20 minutes before the sensory test was carried out was read from the brain waveform,
The value was set as the normal state (100%) of each person. Immediately after the sensory test, the number of appearances of alpha waves and beta waves was calculated every 20 minutes for 5 minutes, and the total number of times was graphed.

FIG. 1 shows the number of appearances of alpha waves, and FIG. 2 shows the ratio of (number of appearances of beta waves) / (number of appearances of alpha waves).
Shown in.

From these results, it was confirmed that the number of appearances of alpha waves was increased by GABA ingestion. Furthermore, the phenomenon of beta waves was also recognized, and it was shown that the ratio of (the number of appearances of beta waves) / (the number of appearances of alpha waves) was 1 or less, suggesting that the healing effect is very large. It

Example 3 Hereinafter, although not particularly limited, foods containing the healing effect composition of the present invention will be mentioned.

Formulation Example of Tableted Food Reduced maltose 71 mg Crystalline cellulose 70 mg Sucrose ester 9 mg The composition of the present invention 170 mg (10 as GABA
0 mg)

[0039]

As described above, according to the present invention, the GA
The BA composition increases the number of appearances of alpha waves,
Furthermore, we have discovered for the first time the effect of reducing the number of beta waves.

By the present discovery, it is possible to provide a composition having a healing effect by directly calming stress irritation and a food containing the composition, and the social contribution of the present invention is great.

[Brief description of drawings]

FIG. 1 is a diagram showing the number of appearances of alpha waves in a second embodiment.

[FIG. 2] (Number of appearances of beta wave) in Example 2 /
It is the figure which showed the ratio of (the appearance frequency of an alpha wave).

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Kim Takehisa             24 Koshinishi-cho, Kichijoin Ishihara-do, Minami-ku, Kyoto-shi, Kyoto Prefecture             No. 5 within Pharma Foods Laboratories, Inc. F-term (reference) 4B018 MD19 MD86 ME14                 4C206 AA01 AA02 FA45 MA01 MA04                       MA10 MA72 NA14 ZA05 ZC41

Claims (4)

[Claims] 1. A healing effect composition comprising GABA as an active ingredient. 2. The healing effect increases the number of appearances of alpha waves, which are brain waves, and simultaneously decreases the number of appearances of beta waves, and the ratio of the number of appearances of beta waves / the number of appearances of alpha waves is smaller than 1. The healing effect composition according to claim 1, which exhibits the effect of 3. The healing effect composition according to claim 1 or 2, which contains GABA in an amount of 5 to 70% by mass. 4. A food or drink comprising the composition for healing effect according to any one of claims 1 to 3.