JP2003171463A - Polylysine and method for producing the same - Google Patents
Polylysine and method for producing the sameInfo
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- JP2003171463A JP2003171463A JP2001372898A JP2001372898A JP2003171463A JP 2003171463 A JP2003171463 A JP 2003171463A JP 2001372898 A JP2001372898 A JP 2001372898A JP 2001372898 A JP2001372898 A JP 2001372898A JP 2003171463 A JP2003171463 A JP 2003171463A
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- polylysine
- present
- molecular weight
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- epl
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polyamides (AREA)
Abstract
Description
【０００１】[0001]
【発明が属する技術分野】本発明はポリリジン及びその
製造方法に関する。TECHNICAL FIELD The present invention relates to polylysine and a method for producing the same.
【０００２】[0002]
【従来の技術】ポリアミノアルキルメタクリレート、ポ
リアミノメチルアクリルアミド、ポリアリルアミン、お
よびポリビニルアミン等の化学合成によるカチオン性ポ
リマーは、カチオン性と分子量効果（分子鎖の凝集力
や、強度や接着性等の物理的特性など）の両方を利用
し、塗料、接着剤、紙力増強剤、化粧品、医薬・抗体等
の固定等に利用されている。2. Description of the Related Art Cationic polymers produced by chemical synthesis such as polyaminoalkylmethacrylate, polyaminomethylacrylamide, polyallylamine, and polyvinylamine have a cationic property and a molecular weight effect (cohesive force of molecular chain, physical properties such as strength and adhesiveness). Characteristics, etc.) and is used for fixing paints, adhesives, paper strengthening agents, cosmetics, pharmaceuticals, antibodies, etc.
【０００３】[0003]
【発明が解決しようとする課題】しかしながら、前述の
化学合成カチオン性ポリマーは、性能は優れるが生分解
性がないうえ安全性の高いポリマーではないことから、
生分解性を有しさらに安全性が高いカチオン性ポリマー
が望まれている。However, the above-mentioned chemically synthesized cationic polymer is excellent in performance but not biodegradable and is not a highly safe polymer.
A cationic polymer having biodegradability and high safety is desired.
【０００４】[0004]
【課題を解決するための手段】本発明者らは前述の従来
技術の課題に鑑み鋭意研究した。その結果、水に可溶な
ポリリジンであって、その１５重量％水溶液の粘度が６
ｍＰａ・ｓｅｃ以上であるポリリジンは、安全性が高く
且つ生分解性を有する優れたカチオン性ポリマーである
こと、さらに、ε−ポリリジンを不活性ガス雰囲気中も
しくは真空中にて加熱することにより本発明のポリリジ
ンを効率よく製造することが可能であることを見出し、
この知見に基づいて本発明を完成させた。DISCLOSURE OF THE INVENTION The inventors of the present invention have made extensive studies in view of the above-mentioned problems of the prior art. As a result, the polylysine was soluble in water, and the viscosity of the 15 wt% aqueous solution was 6%.
The polylysine having mPa · sec or more is an excellent cationic polymer having high safety and biodegradability, and further, by heating ε-polylysine in an inert gas atmosphere or in a vacuum, the present invention can be obtained. Found that it is possible to efficiently produce polylysine of
The present invention has been completed based on this finding.
【０００５】本発明は以下の構成を有する。
（１）水に可溶なポリリジンであって、その１５重量％
水溶液の粘度が６ｍＰａ・ｓｅｃ以上であるポリリジ
ン。The present invention has the following configuration. (1) Water-soluble polylysine, 15% by weight of which
Polylysine having an aqueous solution viscosity of 6 mPa · sec or more.
【０００６】（２）ＳＤＳ−ＰＡＧＥにより測定した分
子量が３万以上であるポリリジンを含有する前記第１項
記載のポリリジン。(2) The polylysine according to the above item 1, containing polylysine having a molecular weight of 30,000 or more as measured by SDS-PAGE.
【０００７】（３）１５％水溶液にした際の該水溶液の
ｐＨが１０．０以上となる前記第１項または第２項記載
のポリリジン。(3) The polylysine according to the above item 1 or 2, wherein the pH of the 15% aqueous solution is 10.0 or more.
【０００８】（４）前記第１項〜第３項の何れか１項記
載のポリリジンの製造方法であって、不活性ガス雰囲気
もしくは真空中において、下記一般式で表されるε−ポ
リリジンを１５０℃以上の温度で熱処理することを特徴
とするポリリジンの製造方法。
（式中ｎは１５〜３００の整数である。）(4) The method for producing polylysine according to any one of items 1 to 3, wherein 150 ε-polylysine represented by the following general formula is added in an inert gas atmosphere or vacuum. A method for producing polylysine, which comprises heat-treating at a temperature of ℃ or higher. (In the formula, n is an integer of 15 to 300.)
【０００９】（５）一般式（１）で表されるε−ポリリ
ジンが、微生物発酵により製造されたε−ポリリジンで
ある請求項４記載のポリリジンの製造方法。(5) The method for producing polylysine according to claim 4, wherein the ε-polylysine represented by the general formula (1) is ε-polylysine produced by microbial fermentation.
【００１０】[0010]
【発明の実施の形態】本発明ポリリジンは、その１５重
量％水溶液の粘度が６ｍＰａ・ｓｅｃ以上であり、本発
明において該粘度は、８ｍＰａ・ｓｅｃ以上であること
が好ましく、さらには１０ｍＰａ・ｓｅｃ以上であるこ
のが好ましい。この範囲であれば、分子鎖の凝集力や、
強度や接着性等の物理的特性などの分子量効果とカチオ
ン性の点において優れた効果を発揮し得る。該粘度の上
限は特に限定されるものではないが、１００ｍＰａ・ｓ
ｅｃ程度までであれば均一な水溶液が得やすい。BEST MODE FOR CARRYING OUT THE INVENTION The polylysine of the present invention has a viscosity of a 15% by weight aqueous solution of 6 mPa · sec or more. In the present invention, the viscosity is preferably 8 mPa · sec or more, more preferably 10 mPa · sec or more. This is preferred. Within this range, the cohesive force of the molecular chains,
It can exert excellent effects in terms of molecular weight effect such as physical properties such as strength and adhesiveness and cationicity. The upper limit of the viscosity is not particularly limited, but 100 mPa · s
If it is up to about ec, a uniform aqueous solution is easily obtained.
【００１１】なお、該粘度の測定方法はＥ型粘度計によ
る。ポリリジン濃度を１５重量％に調整し、Ｅ型粘度計
（東洋精機(株)製）を用い、２５℃で測定する。The viscosity is measured by an E-type viscometer. The polylysine concentration is adjusted to 15% by weight, and the viscosity is measured at 25 ° C. using an E-type viscometer (manufactured by Toyo Seiki Co., Ltd.).
【００１２】本発明のポリリジンは、ＳＤＳ−ＰＡＧＥ
により測定した分子量が３万以上であるポリリジンを含
有することが好ましい。該分子量が３万以上であるポリ
リジンの含有割合は特に限定されるものではないが、本
発明のポリリジンに対して２０〜１００重量％の範囲で
あることが好ましい。この範囲であればカチオン性ポリ
マーとして優れた効果を発揮することができる。The polylysine of the present invention is SDS-PAGE.
It is preferable to contain polylysine having a molecular weight of 30,000 or more as measured by. The content ratio of the polylysine having the molecular weight of 30,000 or more is not particularly limited, but it is preferably in the range of 20 to 100% by weight based on the polylysine of the present invention. Within this range, excellent effects as a cationic polymer can be exhibited.
【００１３】さらに、本発明のポリリジンは、それを濃
度が１５重量％の水溶液にした際の該水溶液のｐＨが１
０．０以上のものであることが好ましい。より好ましく
は１０．３以上であるり、特に好ましくは１０．５以上
である。この範囲であれば高い抗菌力を示し、また、カ
チオン性ポリマーとして優れた効果を発揮することがで
きる。該ｐＨの上限は特に限定されるものではないが、
１１程度が現実的な値であり、カチオンポリマーとして
は十分に機能を発揮する。Further, the polylysine of the present invention has a pH of 1 when it is made into an aqueous solution having a concentration of 15% by weight.
It is preferably 0.0 or more. It is more preferably 10.3 or more, and particularly preferably 10.5 or more. Within this range, high antibacterial activity is exhibited, and an excellent effect as a cationic polymer can be exhibited. The upper limit of the pH is not particularly limited,
A value of about 11 is a realistic value, and it sufficiently exhibits the function as a cationic polymer.
【００１４】本発明のポリリジンの製造方法は特に限定
されるものではないが、不活性ガス雰囲気もしくは真空
中において、下記一般式（１）で表されるε−ポリリジ
ン（以下「ＥＰＬ」と記述する。）を１５０℃以上の温
度で熱処理する方法であれば、本発明のポリリジンを再
現性良くしかも容易に効率よく製造することができる。
The method for producing polylysine of the present invention is not particularly limited, but ε-polylysine represented by the following general formula (1) (hereinafter referred to as “EPL”) in an inert gas atmosphere or vacuum. .) At a temperature of 150 ° C. or higher, the polylysine of the present invention can be produced with good reproducibility and easily and efficiently.
【００１５】本発明においてｎは１５〜３００の整数で
ある。本発明においては、重合度ｎがこの範囲を超えた
ＥＰＬ、若しくは下回ったＥＰＬであっても使用できる
が、重合度ｎが大きいほうが分子量を効率よく大きくで
きるため、できるだけ重合度ｎは大きいものが好ましく
用いられる。しかし、重合度ｎが３００を超えたもの
は、それ自体で十分な分子量を持つため、あえて高分子
量化する必要はないと考えられる。In the present invention, n is an integer of 15 to 300. In the present invention, an EPL having a degree of polymerization n exceeding this range or an EPL having a degree of polymerization lower than this range can be used. However, since the higher the degree of polymerization n is, the more the molecular weight can be increased efficiently, the degree of polymerization n should be as large as possible. It is preferably used. However, a polymer having a degree of polymerization n of more than 300 has a sufficient molecular weight by itself, and thus it is considered unnecessary to increase the molecular weight.
【００１６】上記一般式（１）で表されるＥＰＬは、化
学合成によって得られたものであっても、発酵によって
得られたたものであってもよいが、現在、化学合成品は
微生物発酵のＥＰＬと比較して非常に高価なうえ入手が
容易でないため、本発明においては微生物発酵により製
造されたＥＰＬを好ましく使用することができる。該Ｅ
ＰＬであれば、化学合成品よりもはるかに安価で容易に
入手が可能である。The EPL represented by the above general formula (1) may be either one obtained by chemical synthesis or one obtained by fermentation. Currently, the chemically synthesized product is microbial fermentation. The EPL produced by microbial fermentation can be preferably used in the present invention because it is very expensive and not easily available as compared with the EPL. The E
PL is much cheaper and more easily available than chemically synthesized products.
【００１７】微生物発酵により得られたＥＰＬの中で
も、ストレプトマイセス・アルブラス（Ｓｔｒｅｐｔｏ
ｍｙｃｅｓ・ａｌｂｌｕｓ）や、ストレプトマイセス・
ヌールセイ（Ｓｔｒｅｐｔｏｍｙｃｅｓ・ｎｏｕｒｓｅ
ｉ）に代表されるポリリジン生産菌を培地中にて培養
し、培養後の該培地から分離することによって得られた
ＥＰＬは、食品添加物として使用することが可能なほど
安全性が高いことに加え、その重合度は２５〜３５であ
り、分子量的に十分であるため本発明に好ましく使用す
ることができる。さらに、該ＥＰＬは、フリー体である
ことが好ましい。なお、α―ポリリジンもＥＰＬと同様
に原料として使用可能である。Among EPLs obtained by microbial fermentation, Streptomyces alblas (Strepto)
myces / albus) and Streptomyces
Noorsei (Streptomyces ・ nourse)
The EPL obtained by culturing the polylysine-producing bacterium represented by i) in a medium and separating it from the medium after the culturing is highly safe enough to be used as a food additive. In addition, the degree of polymerization is 25 to 35, and the molecular weight is sufficient, so that it can be preferably used in the present invention. Furthermore, the EPL is preferably a free form. Note that α-polylysine can also be used as a raw material like EPL.
【００１８】熱処理の際に使用することができる不活性
ガスは特に限定されるものではないが、窒素ガス、アル
ゴンガスなどを挙げることができる。またその際のガス
圧は特に限定されるものではないが、安全性の面から
０．１Ｍｐａ以下であることが好ましい。The inert gas that can be used in the heat treatment is not particularly limited, but examples thereof include nitrogen gas and argon gas. The gas pressure at that time is not particularly limited, but is preferably 0.1 MPa or less from the viewpoint of safety.
【００１９】熱処理の際の真空度は、高い程好ましい
が、ロータリーポンプで到達可能な真空度で十分であ
る。The higher the degree of vacuum during the heat treatment, the more preferable, but the degree of vacuum that can be reached by a rotary pump is sufficient.
【００２０】該ＥＰＬを熱処理時の温度は、本発明にお
いては１５０℃以上であるが、好ましくは１５０〜２５
０℃の範囲であり、より好ましくは１８０〜２３０℃の
範囲であり、特に好ましくは１８５〜２００℃の範囲で
ある。この範囲であれば、本発明のポリリジンが効率よ
く得られ、且つ着色や水への不溶化などの品質および収
率の低下が起こりにくい。又、温度を均一にするために
必要に応じて攪拌することが好ましい。The temperature during the heat treatment of the EPL is 150 ° C. or higher in the present invention, but preferably 150 to 25.
It is in the range of 0 ° C., more preferably in the range of 180 to 230 ° C., and particularly preferably in the range of 185 to 200 ° C. Within this range, the polylysine of the present invention can be efficiently obtained, and deterioration in quality and yield such as coloring and insolubilization in water is unlikely to occur. Further, it is preferable to stir as necessary to make the temperature uniform.
【００２１】不活性ガス雰囲気下もしくは真空中におい
てＥＰＬを熱処理する本発明の製造方法であれば、空気
中で熱処理する場合に比べ、ＥＰＬの劣化、着色、水不
溶性ゲルの発生が非常に少ない。According to the manufacturing method of the present invention in which the EPL is heat-treated in an inert gas atmosphere or in vacuum, the EPL is much less deteriorated, colored, and a water-insoluble gel is generated, as compared with the case of heat-treating in air.
【００２２】熱処理する時間は、加熱温度やＥＰＬの量
やその分子量により異なることから、一概に限定できる
ものではないが、５分〜１０時間の範囲であることが好
ましく、より好ましくは１０分〜５時間の範囲であり、
さら好ましくは２０分〜３時間の範囲である。しかし、
原料として用いるＥＰＬの分子量が大きい場合は、その
限りではない。この範囲であれば、本発明のポリリジン
が効率よく得られ、且つ着色や水への不溶化などの品質
および収率の低下が起こりにくい。The time for the heat treatment varies depending on the heating temperature, the amount of EPL and the molecular weight thereof, and is not specifically limited, but is preferably in the range of 5 minutes to 10 hours, more preferably 10 minutes to 10 minutes. 5 hours range,
More preferably, it is in the range of 20 minutes to 3 hours. But,
This does not apply when the molecular weight of EPL used as a raw material is large. Within this range, the polylysine of the present invention can be efficiently obtained, and deterioration in quality and yield such as coloring and insolubilization in water is unlikely to occur.
【００２３】また、本発明の高分子量ＥＰＬは、真空ベ
ントが１個以上付属した単軸又は２軸押し出し機を使用
し、押し出し機のシリンダー内で、混練りしながら加熱
脱気を行うことにより製造することも可能である。この
場合は、加熱時間は滞留時間でコントロールされる。Further, the high molecular weight EPL of the present invention uses a single-screw or twin-screw extruder equipped with one or more vacuum vents, and performs heat deaeration while kneading in the cylinder of the extruder. It is also possible to manufacture. In this case, the heating time is controlled by the residence time.
【００２４】本発明の製造方法はバッチ方式であっても
よく、原料であるＥＰＬを逐次添加する連続方式であっ
ても良い。The production method of the present invention may be a batch system or a continuous system in which EPL as a raw material is sequentially added.
【００２５】本発明のポリリジンは、アミノ基が塩の状
態であっても、本発明ＥＰＬと同等の効果を奏する。Ｅ
ＰＬの塩は、無機塩であっても有機酸塩であってもよ
い。無機塩としては、塩酸、硫酸、およびリン酸などを
挙げることができる。The polylysine of the present invention exhibits the same effect as the EPL of the present invention even when the amino group is in a salt state. E
The salt of PL may be an inorganic salt or an organic acid salt. Examples of the inorganic salt include hydrochloric acid, sulfuric acid, phosphoric acid and the like.
【００２６】また、有機酸塩としては、クエン酸、グル
コン酸、酢酸、酒石酸、乳酸、フマル酸、コハク酸、リ
ンゴ酸、アジピン酸、プロピオン酸、ソルビン酸、安息
香酸のナトリウム塩、カリウム塩、カルシウム塩、鉄塩
等が挙げることができる。これら無機酸および有機酸も
１種または２種以上混合して用いることができる。As the organic acid salt, citric acid, gluconic acid, acetic acid, tartaric acid, lactic acid, fumaric acid, succinic acid, malic acid, adipic acid, propionic acid, sorbic acid, sodium salt of benzoic acid, potassium salt, Examples thereof include calcium salt and iron salt. These inorganic acids and organic acids can also be used alone or in combination of two or more.
【００２７】本発明のポリリジンは、原料に用いたε−
ポリリジンと同じ抗菌性を有し、かつ触媒や架橋剤等の
化合物を全く含まないので、原料に用いたε−ポリリジ
ンと同様に食品保存剤、化粧品、医薬、医療材料、抗菌
素材、さらにカチオン性に加え高分子量を生かして、塗
料、接着剤、紙力増強剤、化粧品、医薬、抗体等の固定
の用途に使用することができる。The polylysine of the present invention is ε-used as a raw material.
It has the same antibacterial properties as polylysine and does not contain any compounds such as catalysts and cross-linking agents at all, so similar to ε-polylysine used as a raw material, food preservatives, cosmetics, pharmaceuticals, medical materials, antibacterial materials, and cationic In addition to its high molecular weight, it can be used for fixing paints, adhesives, paper-strengthening agents, cosmetics, pharmaceuticals, antibodies and the like.
【００２８】[0028]
【実施例】以下、実施例を用いて本発明を詳細に説明す
る。
１．ポリリジンの製造
実験例１
ＥＰＬ１００％粉末５ｇをサンプル瓶に入れ、高温減圧
乾燥機の中で真空とし、該ＥＰＬ粉末を２０分間１８５
℃の状態にした。なお、ＥＰＬ１００粉末は、チッソ株
式会社製ε−ポリリジン２５％水溶液を凍結乾燥するこ
とによって得たものである。EXAMPLES The present invention will be described in detail below with reference to examples. 1. Production Example 1 of Polylysine 1g of EPL100% powder was put in a sample bottle, and vacuum was applied in a high temperature vacuum dryer, and the EPL powder was 185 minutes for 185 minutes.
The temperature was kept at ℃. The EPL100 powder was obtained by freeze-drying a 25% aqueous solution of ε-polylysine manufactured by Chisso Corporation.
【００２９】実験例２
時間を６０分にした以外は実験例１に準じて本発明ポリ
リジンの製造を行った。Experimental Example 2 The polylysine of the present invention was produced according to Experimental Example 1 except that the time was changed to 60 minutes.
【００３０】実験例３
時間を１２０分にした以外は実験例１に準じて本発明ポ
リリジンの製造を行った。Experimental Example 3 The polylysine of the present invention was produced according to Experimental Example 1 except that the time was 120 minutes.
【００３１】実験例４
温度を１５０℃、時間を１８０分にした以外は実験例１
に準じて本発明ポリリジンの製造を行った。Experimental Example 4 Experimental Example 1 except that the temperature was 150 ° C. and the time was 180 minutes.
The polylysine of the present invention was produced according to.
【００３２】実験例５
温度を２００℃、時間を１２０分にした以外は実験例１
に準じて本発明ポリリジンの製造を行った。Experimental Example 5 Experimental Example 1 except that the temperature was 200 ° C. and the time was 120 minutes.
The polylysine of the present invention was produced according to.
【００３３】２．各測定方法
上記各実験例および上記ＥＰＬ１００％粉末について、
下記の項目について測定を行った。結果を表１に示し
た。2. Each measuring method For each of the above experimental examples and the above EPL100% powder,
The following items were measured. The results are shown in Table 1.
【００３４】（溶液粘度の測定）ポリリジン濃度を１５
重量％に調整し、Ｅ型粘度計（東洋精機(株)製）を用
い、２５℃で測定した。(Measurement of solution viscosity) The polylysine concentration was set to 15
It was adjusted to a weight% and measured at 25 ° C. using an E-type viscometer (manufactured by Toyo Seiki Co., Ltd.).
【００３５】（ＳＤＳ−ＰＡＧＥによる分子量の測定）
電気泳動装置（アトー(株)製ＡＥ−６２００）により、
アクリルアミドゲル、標準タンパクマーカーを用いて行
った。(Measurement of molecular weight by SDS-PAGE)
By an electrophoresis device (AE-6200 manufactured by Atto Co., Ltd.),
It was performed using an acrylamide gel and a standard protein marker.
【００３６】アクリルアミドゲルの濃度を１５％とし、
低分子量成分に焦点を絞り、実験例１、２、３、および
ＥＰＬ１００％粉末の分子量測定を行った。結果を図１
に示した。図１から、ＥＰＬ１００％粉末は３万以上の
分子量を含まないが、各実験例のポリリジンは３万以上
の分子量を超える成分を含んでいることがわかる。The concentration of acrylamide gel is set to 15%,
Focusing on the low molecular weight components, the molecular weights of Experimental Examples 1, 2, 3 and EPL100% powder were measured. The result is shown in Figure 1.
It was shown to. It can be seen from FIG. 1 that the EPL 100% powder does not contain a molecular weight of 30,000 or more, but the polylysine of each experimental example contains a component having a molecular weight of 30,000 or more.
【００３７】また、アクリルアミドゲルの濃度を５％と
し、高分子量成分に焦点を絞り、実験例１、２、３、お
よびＥＰＬ１００％粉末の分子量測定を行った。結果を
図２に示した。図２から実験例２および３のポリリジン
は、分子量２０万を超える成分を含んでいることがわか
る。Further, the concentration of the acrylamide gel was set to 5%, the high molecular weight component was focused, and the molecular weights of Experimental Examples 1, 2, 3 and EPL100% powder were measured. The results are shown in Fig. 2. From FIG. 2, it can be seen that the polylysine of Experimental Examples 2 and 3 contains a component having a molecular weight of more than 200,000.
【００３８】（ｐＨの測定）ポリリジン濃度を１５％に
調整し、ｐH測定機（ＭＥＴＴＬＥＲ製 ＴＯＬＥＤＯ
ＭＰ２２０）を用て測定した。(Measurement of pH) The polylysine concentration was adjusted to 15%, and a pH measuring device (METTLER TOLEDO
MP220) was used for the measurement.
【００３９】（抗菌試験：ＭＩＣ）試験菌株として、エ
スケリチア コリ（Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ
（IFO１３５００））、スタフィロコッカス アレウス
（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ（ＩＦ
Ｏ１３２７６））の２菌種を使用し、液体培地法により
測定した。培地としてはニュートリエントブロス培地
（ＮＢ培地）を使用した。(Antibacterial test: MIC) Escherichia coli was used as a test strain.
(IFO13500)), Staphylococcus aureus (IF
O13276)) was used and measured by the liquid culture method. Nutrient broth medium (NB medium) was used as the medium.
【００４０】[0040]
【表１】 [Table 1]
【００４１】[0041]
【発明の効果】水に可溶なポリリジンであって、その１
５重量％水溶液の粘度が６ｍＰａ・ｓｅｃ以上である本
発明のポリリジンは、安全性が高く且つ生分解性を有す
る優れたカチオン性ポリマーであり、従来のＥＰＬと同
様に抗菌剤、化粧品、医薬、抗菌素材に加え、高分子量
効果を生かし、塗料、接着剤、紙力増強剤、化粧品、医
薬・抗体等の固定に使用することができる。また、本発
明の製造方法であれば、本発明のポリリジンを効率よく
製造することが可能である。EFFECT OF THE INVENTION Polylysine soluble in water, which is No. 1
The polylysine of the present invention, in which the viscosity of a 5 wt% aqueous solution is 6 mPa · sec or more, is an excellent cationic polymer having high safety and biodegradability, and like the conventional EPL, antibacterial agents, cosmetics, pharmaceuticals, In addition to the antibacterial material, it can be used for fixing paints, adhesives, paper-strengthening agents, cosmetics, medicines, antibodies, etc. by taking advantage of the high molecular weight effect. Further, the production method of the present invention makes it possible to efficiently produce the polylysine of the present invention.
【図１】 ＥＰＬ１００％粉末、実験例１、２、および
４の分子量 ＳＤＳ−ＰＡＧＥチャート（アクリルアミ
ドゲル濃度１５％）FIG. 1 Molecular weight SDS-PAGE chart of EPL100% powder, Experimental Examples 1, 2, and 4 (acrylamide gel concentration 15%)
【図２】 ＥＰＬ１００％粉末、実験例１、２、および
４の分子量 ＳＤＳ−ＰＡＧＥチャート（アクリルアミ
ドゲル濃度５％）FIG. 2 Molecular weight of EPL100% powder, Experimental Examples 1, 2, and 4 SDS-PAGE chart (acrylamide gel concentration 5%)
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Claims (5)
５重量％水溶液の粘度が６ｍＰａ・ｓｅｃ以上であるポ
リリジン。1. A polylysine soluble in water, which is
Polylysine having a viscosity of 6 mPa · sec or more in a 5 wt% aqueous solution.
が３万以上であるポリリジンを含有する請求項１記載の
ポリリジン。2. The polylysine according to claim 1, which contains polylysine having a molecular weight of 30,000 or more as measured by SDS-PAGE.
ｐＨが１０．０以上となる請求項１または２記載のポリ
リジン。3. The polylysine according to claim 1, wherein the pH of the aqueous solution when it is made into a 15% by weight aqueous solution becomes 10.0 or more.
ジンの製造方法であって、不活性ガス雰囲気もしくは真
空中において、下記一般式で表されるε−ポリリジンを
１５０℃以上の温度で熱処理することを特徴とするポリ
リジンの製造方法。 （式中ｎは１５〜３００の整数である。）4. The method for producing polylysine according to claim 1, wherein the ε-polylysine represented by the following general formula is heated to a temperature of 150 ° C. or higher in an inert gas atmosphere or a vacuum. A method for producing polylysine, which comprises heat-treating. (In the formula, n is an integer of 15 to 300.)
が、微生物発酵により製造されたε−ポリリジンである
請求項４記載のポリリジンの製造方法。5. The method for producing polylysine according to claim 4, wherein the ε-polylysine represented by the general formula (1) is ε-polylysine produced by microbial fermentation.
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