JP2003102467A - Hydrocarbon rich micro-algae and bioremediation method using the same - Google Patents
Hydrocarbon rich micro-algae and bioremediation method using the sameInfo
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- JP2003102467A JP2003102467A JP2001305283A JP2001305283A JP2003102467A JP 2003102467 A JP2003102467 A JP 2003102467A JP 2001305283 A JP2001305283 A JP 2001305283A JP 2001305283 A JP2001305283 A JP 2001305283A JP 2003102467 A JP2003102467 A JP 2003102467A
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- microalgae
- hydrocarbons
- oil
- hydrocarbon
- salt concentration
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】この出願の発明は、炭化水素
を資化できる微細藻類とそれを用いた排水の浄化方法に
関するものである。さらに詳しくは、この出願の発明は
高温度、高塩濃度を含む広い塩濃度範囲、さらには広い
ｐＨ範囲において、炭化水素を資化できる微細藻類と、
それを用いたバイオレメディエーション方法に関するも
のである。TECHNICAL FIELD The invention of this application relates to a microalgae capable of assimilating hydrocarbons and a method for purifying wastewater using the same. More specifically, the invention of this application relates to a microalgae capable of assimilating hydrocarbons at a high temperature, a wide salt concentration range including a high salt concentration, and a wide pH range,
The present invention relates to a bioremediation method using the same.
【０００２】[0002]
【従来技術とその課題】近年大きな社会問題となってい
る海洋汚染では、その発生源として、有害化学物質、油
類、放射性物質、廃棄物の投棄、富栄養価物などが知ら
れている。BACKGROUND OF THE INVENTION Marine pollution, which has become a major social problem in recent years, is known to be a source of harmful chemical substances, oils, radioactive substances, dumping of wastes, and eutrophication.
【０００３】中でも、油類による汚染は、原油生産地域
のみならず、油脂を使用あるいは生産する工場、たとえ
ば食品加工場等の周辺においても問題となっている。ま
た、油田から原油を搬送する際にタンカーの事故が起こ
れば、広い海域に渡り水質汚染が起こり、海鳥、哺乳動
物、魚介類等の周辺環境に甚大な被害をもたらす。例え
ば、海鳥や海洋性の哺乳動物は、羽毛や皮膚表面に疎水
性物質を有し、海水との直接接触を防止して体温を維持
しているが、油が付着すると、この疎水性物質が失われ
るため体温が低下し、生存できなくなる。また、これら
の動物は海水から余分な塩分を排出しながら水分を補給
するが、油の付着によりこの機能も失われるため、脱水
症状を起こす。さらに、海草や魚介類は、表面に油が付
着することにより光合成や呼吸ができなくなり、死滅す
る。Above all, pollution by oils is a problem not only in the crude oil production area but also in factories that use or produce oils and fats, such as food processing plants and the like. In addition, if a tanker accident occurs during the transportation of crude oil from an oil field, water pollution will occur over a wide area of the ocean, and the surrounding environment such as seabirds, mammals, and seafood will be seriously damaged. For example, seabirds and marine mammals have hydrophobic substances on their feathers and skin surfaces, and maintain their body temperature by preventing direct contact with seawater. They lose their body temperature and lose their viability. In addition, these animals replenish their water while discharging excess salt from seawater, but this function is lost due to the adhesion of oil, resulting in dehydration. Furthermore, seagrass and seafood die due to the lack of photosynthesis and respiration due to oil adhering to the surface.
【０００４】従来、タンカーや油田の事故により一過的
に水域に流出した原油や石油は、オイルフェンスにより
その広がりを防止し、界面活性剤や有機溶剤からなる油
処理剤を散布することにより、油が水と混合するように
して処理していた。しかし、油処理剤を使用する方法で
は、流出した油類は、小さな油滴となり、水と混合さ
れ、やがて海洋中に希釈されてその被害の規模を縮小で
きるものの、根本的な解決にはならない。また、工業排
水等に少量含まれる油類の処理には適用できるものでは
なかった。Conventionally, crude oil and petroleum that have transiently spilled into a water area due to an accident in a tanker or an oil field are prevented from spreading by an oil fence, and an oil dispersant composed of a surfactant or an organic solvent is sprayed, The oil was treated so that it mixed with water. However, with the method using dispersants, the spilled oil becomes small oil droplets, which are mixed with water and eventually diluted in the ocean to reduce the damage, but this is not a fundamental solution. . In addition, it was not applicable to the treatment of oils contained in industrial wastewater in small amounts.
【０００５】そこで、光や熱により油を分解する方法
や、活性炭や薬剤を用いた物理化学的吸着等の方法も提
案され、一部で使用されている。しかし、これらの方法
は、光や熱、あるいは大量の試薬を用いる上、浄化のた
めの機械設備等を必要とするため、コストが高い、タン
カー事故等の遠洋における油の処理には適用しにくい、
広範に渡る汚染には対応できないなどの問題があった。Therefore, a method of decomposing oil by light or heat and a method of physicochemical adsorption using activated carbon or chemicals have been proposed and used in part. However, since these methods use light, heat, or a large amount of reagents, and require mechanical equipment for purification, etc., they are expensive and difficult to apply to oil treatment in the pelagic sea such as tanker accidents. ,
There were problems such as being unable to cope with widespread pollution.
【０００６】そこで、近年、環境適応性の高い方法とし
て、バイオレメディエーションが注目されている。バイ
オレメディエーションは、浄化機能を有する各種の微生
物を用いて、環境汚染の著しい水域や土壌における油等
の炭化水素や富栄養価物等を乳化、分解、資化する方法
であり、常温・常圧で行うためにエネルギーをほとんど
必要としない。したがって、コストが低い、汚染の現場
で直接的に処理を行うことができる、油田や工場の操業
中であっても浄化処理を行うことができる、広範囲に渡
る汚染を浄化することができる、などの利点を有する。Therefore, in recent years, bioremediation has attracted attention as a method having high environmental adaptability. Bioremediation is a method of emulsifying, decomposing, and assimilating hydrocarbons such as oil and nutritive substances in water and soil with significant environmental pollution using various microorganisms that have a purification function. Requires little energy to do in. Therefore, the cost is low, the treatment can be performed directly at the pollution site, the purification treatment can be performed even during the operation of the oil field or the factory, the contamination over a wide range can be eliminated, etc. Have the advantage of.
【０００７】このようなバイオレメディエーションで用
いられる微生物としては、各種のものが知られている。
たとえば、Agrobacterium属やRodococcas属の油分解性
バクテリアが、高い炭化水素分解性を示すものとして報
告されている（Biosci.Biotechnol.Biochem., 59, 11,
2159-2161 (1995)）。また、石油分解酵母（Candidamal
tosa）は、低いｐＨ領域においても高い活性を示すもの
として報告されている（Wolf, K., ed, Non-convention
al Yeast in Biotechnology, pp.426-430 (1996)）。し
かし、これらのバクテリアは、低いｐＨにおける分解能
が低く、酵母は、３０℃以上の高温およびｐＨ６．５以
上では活性が著しく低下するため、高温地域では使用で
きないという問題があった。Various microorganisms are known as the microorganisms used in such bioremediation.
For example, oil-degrading bacteria of the genus Agrobacterium and the genus Rodococcas have been reported as showing high hydrocarbon degradability (Biosci.Biotechnol.Biochem., 59, 11, 11.
2159-2161 (1995)). In addition, petroleum degrading yeast (Candidamal
tosa) has been reported as showing high activity even in a low pH region (Wolf, K., ed, Non-convention).
al Yeast in Biotechnology, pp.426-430 (1996)). However, these bacteria have low resolution at low pH, and yeast has a problem that it cannot be used in a high temperature region because its activity is remarkably reduced at a high temperature of 30 ° C. or higher and at a pH of 6.5 or higher.
【０００８】さらに、酵母様の形態を示す真核微生物で
あるPrototheca属、中でもPrototheca hydrocarboneaや
Prototheca zopfii等の緑藻が石油分解性を有すること
が報告されているが、これらの育成及び分解の条件は、
ｐＨ３〜９、塩化ナトリウム濃度０．８〜２．４重量
％、２５℃以下であり、限られた環境下においてのみ、
活性を示す。とくに３０℃以上を超える温度では、生育
及び油成分の分解は不可能とされていた（Appl.Enviro
n.Microb., p.333-336 (1983); System.Appl.Microbio
l.5, 119-123 (1984)）。一般的に海水の塩濃度は約
３．５重量％であるため、従来報告されているProtothe
ca属の藻類は、海水からの油成分の分解・浄化には使用
できない。また土壌汚染に対しては、使用できる温度と
塩濃度の範囲が限定されているため、沿岸地域や気温の
高い地域では使用できなかったのが実情である。[0008] Furthermore, the genus Prototheca, which is a eukaryotic microorganism exhibiting a yeast-like morphology, among them Prototheca hydrocarbonea and
It has been reported that green algae such as Prototheca zopfii have petroleum degradability, but the conditions for growing and degrading them are as follows:
pH 3-9, sodium chloride concentration 0.8-2.4% by weight, 25 ° C or less, only under limited environment,
Shows activity. Especially at temperatures above 30 ° C, growth and decomposition of oil components were not possible (Appl.Enviro
n.Microb., p.333-336 (1983); System.Appl.Microbio
l.5, 119-123 (1984)). Generally, salt concentration of seawater is about 3.5% by weight.
Algae of the genus ca cannot be used for the decomposition and purification of oil components from seawater. As for soil pollution, the usable temperature and salt concentration range is limited, so it cannot be used in coastal areas or high temperature areas.
【０００９】したがって、この出願の発明は、以上のと
おりの問題点を解決し、広範囲なｐＨ、温度および塩濃
度の土壌や河川、湖沼、海洋等において実施できるバイ
オレメディエーションの方法と、それに適用するための
微生物として、高温度、高塩濃度を含む広い塩濃度範
囲、あるいは広いｐＨ範囲においても炭化水素を資化で
きる微生物を提供することを課題としている。Therefore, the invention of this application solves the problems described above, and can be applied to a bioremediation method which can be carried out in soil, rivers, lakes, oceans, etc. having a wide range of pH, temperature and salt concentrations. As a microorganism for the purpose, it is an object to provide a microorganism capable of assimilating hydrocarbon even in a wide salt concentration range including high temperature and high salt concentration, or in a wide pH range.
【００１０】[0010]
【課題を解決するための手段】この出願の発明は、以上
のとおりの課題を解決するものとして、まず、第１に
は、炭化水素を資化する微細藻類であって、３０〜４０
℃の温度領域において炭化水素を資化できることを特徴
とする微細藻類を提供する。[Means for Solving the Problems] The invention of this application is intended to solve the above-mentioned problems. Firstly, a microalgae that assimilates hydrocarbons is used.
A microalgae characterized by being able to assimilate hydrocarbons in the temperature range of ° C.
【００１１】この出願の発明は、第２には、０〜２Ｍの
塩濃度範囲で炭化水素を資化できる前記の微細藻類を、
第３には、ｐＨ３〜９の範囲で炭化水素を資化できる前
記いずれかの微細藻類を、第４には、バイオサーファク
タント能を有する前記いずれかの微細藻類を提供する。Secondly, the invention of this application provides the above-mentioned microalgae capable of assimilating hydrocarbons in a salt concentration range of 0 to 2M,
Third, it provides any of the above microalgae capable of assimilating hydrocarbons in the range of pH 3 to 9, and fourthly, provides any one of the above microalgae having biosurfactant ability.
【００１２】この出願の発明は、また、第５には、前記
の微細藻類がPrototheca属の微細藻類であること、およ
び、第６には、前記のPrototheca属に属する微細藻類が
Prototheca zopfii RND-16（Ferm P-18543）であること
をその態様として提供する。The invention of this application is, fifthly, that the above-mentioned microalgae belong to the genus Prototheca, and sixthly, that the microalgae belong to the genus Prototheca.
Prototheca zopfii RND-16 (Ferm P-18543) is provided as an embodiment.
【００１３】さらに、第７には、この出願の発明は、少
なくとも前記いずれかの微細藻類を用いることを特徴と
するバイオレメディエーション方法を提供する。Further, seventhly, the invention of this application provides a bioremediation method characterized by using at least one of the above microalgae.
【００１４】そして、この出願の発明は、第８には、炭
化水素を含有する排水を浄化する方法であって、少なく
とも、排水に前記いずれかの微細藻類を接触させる工程
を有することを特徴とするバイオレメディエーション方
法を、第９には、炭化水素によって汚染された土壌を浄
化する方法であって、少なくとも、汚染土壌に前記いず
れかの微細藻類を接触させる工程を有することを特徴と
するバイオレメディエーション方法をも提供する。An eighth aspect of the invention of this application is a method for purifying wastewater containing hydrocarbons, which comprises at least a step of bringing the microalgae into contact with the wastewater. Ninth, there is provided a bioremediation method for purifying soil contaminated with hydrocarbons, which comprises at least a step of bringing the microalgae into contact with contaminated soil. A method is also provided.
【００１５】[0015]
【発明の実施の形態】この出願の発明者らは、鋭意研究
により、静岡県伊豆蓮台寺温泉の４０℃および３５．５
℃の排水中から分離した酵母様微生物１２株の中に、広
範囲なｐＨ領域および塩濃度において炭素水素を資化す
る微細藻類を見出し、本願発明に至ったものである。具
体的には、本願発明の微細藻類は、３０〜４０℃の温度
範囲、ｐＨ３〜９の範囲、０〜２Ｍの塩濃度範囲で炭化
水素を資化できるものである。BEST MODE FOR CARRYING OUT THE INVENTION The inventors of the present application have earnestly studied, and at 40 ° C. and 35.5 at Izurendai-ji Onsen, Shizuoka Prefecture.
The present invention has been accomplished by finding microalgae that assimilate carbon hydrogen in a wide range of pH range and salt concentration in 12 yeast-like microorganism strains isolated from wastewater at 0 ° C. Specifically, the microalgae of the present invention can assimilate hydrocarbons in a temperature range of 30 to 40 ° C, a pH range of 3 to 9, and a salt concentration range of 0 to 2M.
【００１６】また、後述の実施例からも明らかなよう
に、本願発明の微細藻類は、炭化水素を資化する際に、
界面活性剤成分を分泌し、石油等の炭化水素成分を乳化
して油滴を形成した後、その油滴に入り込み増殖する。
すなわち、この発明の微細藻類は、バイオサーファクタ
ント能を有するものでもある。Further, as is clear from the examples described below, the microalgae of the present invention, when utilizing hydrocarbons,
After secreting a surfactant component and emulsifying a hydrocarbon component such as petroleum to form an oil droplet, it enters the oil droplet and proliferates.
That is, the microalgae of the present invention also have biosurfactant ability.
【００１７】本発明者らは、この微細藻類についてさら
に研究を進め、18SrDNAの塩基配列による系統解析から
この微細藻類がPrototheca zopfii var. hydrocaronea
に属することを明らかにした。また、この微細藻類は、
Prototheca zopfiiと１００％の確率で単系統を形成す
ると同時に、同じ従属栄養のPrototheca属であるProtot
heca wickerhamiiよりも独立栄養のクロレラであるAuxe
nochlorella protothecoidesに近縁であることも明らか
にされている。The present inventors further proceeded with research on this microalgae, and from the phylogenetic analysis of the nucleotide sequence of 18S rDNA, this microalgae was identified as Prototheca zopfii var. Hydrocaronea.
Clarified that it belongs to. Also, this microalgae
Prototheca zopfii forms a single line with 100% probability, and at the same time, Prototheca, a genus of Prototheca with the same heterotrophic
Auxe, a chlorella more autotrophic than heca wickerhamii
It has also been revealed that it is closely related to nochlorella protothecoides.
【００１８】さらに、この出願の発明の微細藻類は、従
来報告されているPrototheca zopfiiには見られない次
のような特徴を有するものである。Furthermore, the microalgae of the invention of this application has the following characteristics not found in the previously reported Prototheca zopfii.
【００１９】（ａ）炭化水素を資化してエタノールとす
る
（ｂ）一般培地で、４０℃以上の高い温度範囲で増殖す
る
（ｃ）炭化水素を炭素源とする培地で、４０℃までの高
い温度範囲で増殖する
（ｄ）静置培養における炭化水素の最大資化速度が従来
公知の菌株の２倍以上である
そして、本願の発明者らは、以上のとおりの特性を有す
る微細藻類を特許寄託している（受託番号：FERM P-185
43）。(A) Assimilates hydrocarbons to ethanol (b) Proliferates in a general medium in a high temperature range of 40 ° C. or higher (c) In a medium containing hydrocarbons as a carbon source, high temperatures up to 40 ° C. (D) The maximum assimilation rate of hydrocarbons in static culture that grows in the temperature range is more than twice that of conventionally known strains. And, the inventors of the present application patented microalgae having the above characteristics. Deposited (Accession number: FERM P-185
43).
【００２０】したがって、この出願の発明の微細藻類を
原油の流出した海洋や土壌、油等の炭化水素成分を含有
する排水や土壌と接触させれば、迅速にかつ効率よく、
炭化水素成分が分解され、浄化処理が行える。この出願
の発明の排水および汚染土壌の浄化方法では、微細藻類
と排水または土壌の接触方法はとくに限定されず、浄化
対象の地域や水域に前記微細藻類を散布する方法や、排
水や汚泥を該微細藻類を含有する浄化槽に導入する方法
等のさまざまな方法が適用できる。もちろん、これらの
浄化方法では、排水または土壌を微細藻類と接触させる
工程以外に、洗浄、沈降、ろ過、攪拌、培養等の様々な
工程を有していてもよい。Therefore, when the microalgae of the invention of this application is brought into contact with the ocean or soil from which crude oil has flown out, or the drainage or soil containing hydrocarbon components such as oil, it is possible to quickly and efficiently
Hydrocarbon components are decomposed and purification treatment can be performed. In the method of purifying drainage and contaminated soil of the invention of this application, the method of contacting the microalgae with the drainage or the soil is not particularly limited, and a method of spraying the microalgae in the area or body of water to be purified, drainage or sludge is used. Various methods such as a method of introducing into a septic tank containing microalgae can be applied. Of course, these purification methods may have various steps such as washing, sedimentation, filtration, stirring, and culture in addition to the step of contacting drainage or soil with microalgae.
【００２１】この出願の発明の微細藻類を用いるバイオ
レメディエーション方法は、従来公知の微生物を用いる
方法に比べ、温度、塩濃度、ｐＨ等の浄化条件が広いた
め、海洋や高温地域、低ｐＨ土壌、沿岸地域等のあらゆ
る環境において適用できる。The bioremediation method using microalgae of the invention of this application has a wider purification condition such as temperature, salt concentration, pH, etc. than the method using conventionally known microorganisms. It can be applied in all environments such as coastal areas.
【００２２】なお、この出願の発明の微細藻類は、炭化
水素を資化するだけでなく、グルコースからガスを発生
する嫌気代謝を行うことも明らかになっている。そし
て、このようなガスの発生を伴う糖の発酵（エタノール
発酵）は、従来のPrototheca属藻類においては知られて
いなかった性質である。It has been clarified that the microalgae of the invention of this application not only assimilate hydrocarbons, but also perform anaerobic metabolism to generate gas from glucose. Then, the fermentation of sugar (ethanol fermentation) accompanied by the generation of such gas is a property that has not been known in conventional Prototheca algae.
【００２３】以下、実施例を示してこの出願の発明につ
いてさらに詳細に説明する。もちろん、この出願の発明
は、以下の実施例に限定されるものではないことはいう
までもない。Hereinafter, the invention of this application will be described in more detail with reference to Examples. Needless to say, the invention of this application is not limited to the following examples.
【００２４】[0024]
【実施例】＜実施例１＞静岡県の伊豆連光寺温泉の４０
℃および３５．５℃の排水から分離した微細藻類Protot
heca zopfii RND-16株（FERM P-18543）を４％（v/v）
ｎ−ヘキサデカン（Ｃ16：和光純薬製、#080-03685）を
含むＹＮＢ培地（Difco製、#291940）中に添加し、２
５、３０、３５℃でそれぞれ２０日間培養した。[Example] <Example 1> 40 at Izurenkoji hot spring in Shizuoka prefecture
Microalgae Protot isolated from wastewater at ℃ and 35.5 ℃
4% of heca zopfii RND-16 strain (FERM P-18543) (v / v)
Add to a YNB medium (Difco, # 291940) containing n-hexadecane (C 16 : Wako Pure Chemical Industries, # 080-03685), and add 2.
The culture was performed at 5, 30, and 35 ° C. for 20 days, respectively.
【００２５】培養開始から微細藻類の数をトーマ血球計
により数えた。From the start of culture, the number of microalgae was counted by a Toma hemacytometer.
【００２６】図１に各温度における増殖曲線を示した。FIG. 1 shows the growth curves at each temperature.
【００２７】これより、本願の微細藻類がＮ−ヘキサデ
カンの存在下で３５℃においても良好な増殖を示し、そ
の速度が２５℃よりも明らかに大きいことが確認され
た。From the above, it was confirmed that the microalgae of the present invention showed good growth even at 35 ° C in the presence of N-hexadecane, and the rate was obviously higher than 25 ° C.
【００２８】また、Ｎ−ヘキサデカンを活発に資化、分
解している微細藻類を顕微鏡で観察し、写真を撮影し
た。図２に、微細藻類の顕微鏡写真を示した。Further, microalgae, which actively assimilate and decompose N-hexadecane, were observed with a microscope and photographed. FIG. 2 shows a micrograph of microalgae.
【００２９】図２より、本願発明の微細藻類は、界面活
性剤成分を分泌し、水中のｎ−ヘキサデカンを乳化して
油滴を形成した後その油滴中に入り込み、ｎ−ヘキサデ
カンを分解、資化することが確認された。
＜実施例２＞実施例１と同様に、微細藻類Prototheca z
opfii RND-16株（FERM P-18543）をテトラデカン
（Ｃ14）、ペンタデカン（Ｃ15）、ヘキサデカン
（Ｃ16）、ヘプタデカン（Ｃ17）中にそれぞれ添加し
（１％(v/v)）、３５℃で培養した。From FIG. 2, the microalgae of the present invention secrete a surfactant component, emulsify n-hexadecane in water to form oil droplets, and then enter the oil droplets to decompose n-hexadecane, It was confirmed to be assimilated. <Example 2> Similar to Example 1, the microalga Prototheca z
opfii RND-16 strain (FERM P-18543) was added to tetradecane (C 14 ), pentadecane (C 15 ), hexadecane (C 16 ), and heptadecane (C 17 ) (1% (v / v)), Cultured at 35 ° C.
【００３０】培養液中に残存する各ｎ−アルカンの濃度
をガスクロマトグラフにより分析した。図３（ａ）に培
養前、図３（ｂ）に１０日間培養後の結果を示した。The concentration of each n-alkane remaining in the culture solution was analyzed by gas chromatography. The results before culturing are shown in FIG. 3 (a) and the results after culturing for 10 days are shown in FIG. 3 (b).
【００３１】図３より、培養１０日目で４種類のｎ−ア
ルカンがほとんど分解されることが確認された。From FIG. 3, it was confirmed that four kinds of n-alkanes were almost decomposed on the 10th day of culture.
【００３２】以上の試験結果から、この出願の発明の微
細藻が、従来知られているPrototheca zopfiiとは全く
異なる性質を示す新規微生物であることが示された。ま
た、この微細藻類は、高温度範囲でも炭化水素である各
種ｎ−アルカンを迅速に、効率よく分解、浄化できるこ
とが確認された。From the above-mentioned test results, it was shown that the microalgae of the invention of this application is a novel microorganism exhibiting completely different properties from the conventionally known Prototheca zopfii. It was also confirmed that the microalgae can decompose and purify various n-alkanes that are hydrocarbons rapidly and efficiently even in a high temperature range.
【００３３】[0033]
【発明の効果】以上詳しく説明したとおり、この出願の
発明により、新規な炭化水素資化微細藻類と、それを用
いた炭化水素含有排水の浄化方法が提供される。この微
細藻類は、広範囲なｐＨ、温度及び塩濃度において炭化
水素成分を分解浄化できるものであり、油田や原油タン
カーの事故等によって油が流出した海洋や土壌や、炭化
水素成分を含有する工業排水等のバイオレメディエーシ
ョンに適用できる微生物として有用性が高い。As described in detail above, the invention of this application provides a novel hydrocarbon-utilizing microalgae and a method for purifying hydrocarbon-containing wastewater using the same. This microalgae is capable of decomposing and purifying hydrocarbon components over a wide range of pH, temperature and salt concentration, and the ocean and soil where oil spilled due to accidents in oil fields and crude oil tankers, and industrial wastewater containing hydrocarbon components. It is highly useful as a microorganism applicable to bioremediation such as.
【図１】この出願の発明の実施例において、本願発明の
微細藻類をｎ−ヘキサデカン存在下、各温度で培養した
際の増殖曲線を示した図である。FIG. 1 is a diagram showing a growth curve when the microalgae of the present invention were cultured at each temperature in the presence of n-hexadecane in an example of the invention of this application.
【図２】この出願の発明の微細藻類がヘキサデカンを資
化、分解している様子の顕微鏡写真を示した図である。FIG. 2 is a view showing a micrograph showing how the microalgae of the invention of this application utilize and decompose hexadecane.
【図３】この出願の発明の実施例において、本願発明の
微細藻類を各種ｎ−アルカンの存在下に培養した際のガ
スクロマトグラフを示した図である。（ａ：培養前、
ｂ：培養後）FIG. 3 is a diagram showing a gas chromatograph when the microalgae of the present invention were cultured in the presence of various n-alkanes in the examples of the invention of this application. (A: before culturing,
b: after culture)
───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 4B065 AA83X BA22 BC02 BC50 CA54 4D004 AA41 AB05 CA17 CA35 CC20 DA03 DA06 DA10 4D040 CC03 ─────────────────────────────────────────────────── ─── Continued front page F-term (reference) 4B065 AA83X BA22 BC02 BC50 CA54 4D004 AA41 AB05 CA17 CA35 CC20 DA03 DA06 DA10 4D040 CC03
Claims (9)
３０〜４０℃の温度範囲において炭化水素を資化できる
ことを特徴とする微細藻類。1. A microalgae that utilizes hydrocarbons,
Microalgae characterized by being able to assimilate hydrocarbons in a temperature range of 30 to 40 ° C.
できる請求項１の微細藻類。2. The microalgae according to claim 1, which is capable of assimilating hydrocarbons in a salt concentration range of 0 to 2M.
る請求項１または２のいずれかの微細藻類。3. The microalgae according to claim 1, which is capable of assimilating a hydrocarbon in a pH range of 3 to 9.
項１ないし３のいずれかの微細藻類。4. The microalgae according to claim 1, which has a biosurfactant ability.
ないし４のいずれかの微細藻類。5. A microalgae of the genus Prototheca.
A microalgae according to any one of 1 to 4.
otheca zopfii RND-16（Ferm P-18543）である請求項５
の微細藻類。6. A microalgae belonging to the genus Prototheca is Prot
otheca zopfii RND-16 (Ferm P-18543).
Microalgae.
の微細藻類を用いることを特徴とするバイオレメディエ
ーション方法。7. A bioremediation method, which comprises using the microalgae according to any one of claims 1 to 6.
であって、少なくとも、排水に請求項１ないし６のいず
れかの微細藻類を接触させる工程を有することを特徴と
する請求項７のバイオレメディエーション方法。8. A method for purifying a wastewater containing a hydrocarbon, which comprises at least a step of contacting the wastewater with the microalgae according to any one of claims 1 to 6. Mediation method.
する方法であって、少なくとも、汚染土壌に請求項１な
いし６のいずれかの微細藻類を接触させる工程を有する
ことを特徴とする請求項７のバイオレメディエーション
方法。9. A method for purifying soil contaminated with hydrocarbons, which comprises at least the step of contacting the contaminated soil with the microalgae according to any one of claims 1 to 6. Bioremediation method.
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