JP2003102460A - Hybridization bag, method of hybridization, and hybridization device using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a hybridization bag enabling hybridization to be automated and easy to handle. SOLUTION: This hybridization bag, which is formed of a plastic sheet 8 with one end 8a thereof sealed, has the following structure: a pair of thick- walled holding parts 9 extending in the longitudinal direction along both rims are provided, and perforations 10 are furnished respectively along the longitudinal direction on the pair of holding parts 9, and one of the pair of holding parts 9 is provided with one or more solution injection parts 11a and/or 11b threadable with pin(s) via which a solution is to be injected.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hybridization bag, a hybridization method and a hybridization apparatus using the same, and more specifically, a hybridization bag which can be automated and easy to handle. The present invention relates to a hybridization bag, a hybridization method using the same, and a hybridization device.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Furthermore, in recent years, cells at different positions on the surface of a carrier such as a slide glass plate or a membrane filter,
Viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA
A specific binding substance, such as A, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Forming a large number of independent spots, then cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c
A substance derived from a living body, such as DNA, DNA, or mRNA, which is collected from the living body by extraction or isolation, or which is further subjected to a chemical treatment, a chemical modification, or the like, such as a fluorescent substance or a dye. A substance labeled with a labeling substance is bound to a specific binding substance by hybridization or the like, to a microarray that is specifically bound,
A microarray analysis system has been developed which irradiates excitation light and photoelectrically detects light such as fluorescence emitted from a labeling substance such as a fluorescent substance or a dye to analyze a substance derived from a living body. According to this microarray analysis system,
A large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and the substance of biological origin labeled with the labeling substance is hybridized, thus In time, there is an advantage that it is possible to analyze a substance of biological origin.

In addition, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, RNAs, etc. derived from living organisms are located at different positions on the surface of a carrier such as a membrane filter. The specific binding substance that can specifically bind to the substance of which the base sequence, base length, composition, etc. are known is dropped using a spotter device to form a large number of independent spots. , Then, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc., collected from the living body by extraction or isolation, or
Furthermore, a substance derived from a living body, which has been subjected to a chemical treatment, a chemical modification, or the like, and which is labeled with a radioactive labeling substance, is specifically bound to a specific binding substance by hybridization or the like. Macro array,
The stimulable phosphor layer containing the stimulable phosphor is brought into close contact with the stimulable phosphor sheet, and the stimulable phosphor layer is exposed.
After that, the photostimulable phosphor layer is irradiated with excitation light, the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected, and biochemical analysis data is generated. A macroarray analysis system using a radiolabeled substance for analyzing is also developed.

[0008]

In a microarray analysis system or a macroarray analysis system, a solution containing a specific binding substance is dropped at different positions on the surface of a biochemical analysis unit such as a membrane filter, and a large number of A specific substance contained in the spot-like region is a biological substance labeled with a radioactive substance, a fluorescent substance, or a labeling substance that causes chemiluminescence when contacted with a chemiluminescent substrate. By hybridizing with the binding substance, the specific binding substance is selectively labeled, and the stimulable phosphor layer of the stimulable phosphor sheet is changed by the radioactive labeling substance selectively contained in a large number of spot-shaped regions. Exposed, the exposed photostimulable phosphor layer, scanned by excitation light, to excite the photostimulable phosphor contained in the photostimulable phosphor layer, Photostimulated photostimulable light emitted from the photostimulable phosphor is generated photoelectrically to generate data for biochemical analysis, or a large number of spot-shaped regions are scanned with excitation light to form a large number of spot-shaped regions. Exciting the fluorescent substance selectively contained, photoelectrically detecting the fluorescence emitted from the fluorescent substance, to generate biochemical analysis data, or
It is required to generate data for biochemical analysis by contacting a chemiluminescent substrate with a labeling substance selectively contained in many spot-like regions and photoelectrically detecting chemiluminescence emitted from the labeling substance. Has been done.

In the case of hybridizing a specific binding substance with a substance of biological origin, conventionally, an experimenter manually performed biochemistry such as a membrane filter in which a large number of spot-shaped regions containing the specific binding substance were formed. The analysis unit is placed in a hybridization bag, and a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contacting with a radioactive labeling substance, a fluorescent substance, or a chemiluminescent substrate is placed in the hybridization bag. Add a hybridization solution containing the product, and apply vibration to the hybridization bag to move the substance of biological origin by convection or diffusion to hybridize the specific binding substance and the substance of biological origin. Remove from hybridization bag and fill with wash solution Placed in the vessel for cleaning was generally.

However, the experimenter manually puts the unit for biochemical analysis in the hybridization bag, adds the hybridization solution, and vibrates the hybridization bag so that the specific binding substance and the biological origin can be obtained. When hybridizing the substance of
There is a problem in that it is unavoidable that the results of hybridization vary depending on the experimenter and the reproducibility decreases, and that even the same experimenter may decrease the reproducibility.

Further, the hybridization bag is
Since it is formed of an extremely thin sheet, there is a problem that handling is difficult.

Therefore, an object of the present invention is to provide a hybridization bag which can automate the hybridization and is easy to handle, a hybridization method and a hybridization apparatus using the same.

[0013]

The object of the present invention is to:
A hybridization bag formed of a plastic sheet and having one end sealed, along both edges, a pair of thick-walled holding portions extending in the longitudinal direction, each of the pair of holding portions, A perforation is formed along the longitudinal direction, and at least one solution injection part through which a solution injection pin can be inserted is formed in one of the pair of holding parts. To be achieved.

According to the present invention, a pair of thick-walled holding portions extending in the longitudinal direction are formed along both edges of the hybridization bag, and the pair of thick-walled holding portions respectively extend in the longitudinal direction. Since the perforations are formed, the hybridization bag can be mechanically and easily transported in the longitudinal direction by engaging the drive mechanism of the hybridization device with the perforations. Since at least one solution injection part through which a pin for injecting a solution can be inserted is formed in one of the pair of holding parts formed along both edges of the hybridization bag, at least 1
When the two solution injection parts are configured to close the part where the pin is inserted after withdrawing the pin where the part is inserted, for example, a hybridization bag is provided with a specific binding substance whose structure or property is known. A plurality of absorptive regions including, after accommodating the biochemical analysis unit formed separated from each other, at least one solution injection unit,
The hybridization solution injection pin for inserting the hybridization solution is inserted, the hybridization solution is injected into the hybridization bag, and the squeeze roller means sandwiches the hybridization bag. Driving the drive mechanism engaged with the perforation, the hybridization bag is moved relative to the squeeze roller means, the hybridization solution is agitated, pre-hybridization is performed and at least one solution injection part The probe solution injection pin for injecting the probe solution is inserted, the probe solution is injected into the hybridization bag, and the hybridization bag is sandwiched by the squeeze roller means, which is formed in the pair of holding portions. Drive the drive mechanism engaged with the perforation to move the hybridization bag with respect to the squeeze roller means and agitate the solution in which the probe solution is added to the hybridization solution. It can be carried out and thus allows the hybridization process to be automated, allowing the experimenter to significantly improve the reproducibility of the hybridization without variations in the hybridization results. At the same time, it is possible to realize a significant labor saving.

Further, according to the present invention, when at least one solution injecting portion is formed with a hole in the portion into which the pin has been inserted after withdrawing the inserted pin, for example, two solution injecting portions are injected. By providing the part, one of the solution injection parts located distal from the sealed one end is inserted with the hybridization solution injection pin, and the hybridization solution is injected into the hybridization bag, and then one solution is injected. A drive mechanism in which the hybridization bag is sealed between the injection part and the other solution injection part, the hybridization bag is held by the squeeze roller means, and the perforation formed in the pair of holding parts is engaged. To move the hybridization bag to the squeeze roller means, and After stirring the lysis solution, pre-hybridization is performed, insert the probe solution injection pin into the other solution injection part, inject the probe solution into the hybridization bag, and then seal with the other solution injection part. Between one end
The hybridization bag is sealed, the squeeze roller means is sandwiched by the squeeze roller means, and the drive mechanism engaged with the perforations formed on the pair of holding portions is driven, and the hybridization bag is moved to the squeeze roller means. In contrast, by allowing the solution to move and stirring the solution in which the probe solution is added to the hybridization solution, it is possible to automate the hybridization process, thus allowing the experimenter to The result of the hybridization does not vary, the reproducibility of the hybridization can be significantly improved, and the significant labor saving can be realized.

[0016] In a preferred aspect of the present invention, the at least one solution injecting section is configured such that the portion in which the pin is inserted is closed after the pin in which it is inserted is pulled out.

According to a preferred embodiment of the present invention, the at least one solution injecting section is configured so that the portion in which the pin is inserted is closed after withdrawing the inserted pin. After injecting the solution or probe solution into the hybridization bag, it is not necessary to seal the hybridization bag before stirring the solution.
The hybridization solution injection probe pin and the probe solution injection pin can be inserted into each of the two solution injection parts to inject the hybridization solution and probe solution into the hybridization bag, thus simplifying the hybridization process. Can be converted.

[0018] In a further preferred aspect of the present invention, the at least one solution injection portion is formed of elastic rubber.

In a preferred aspect of the present invention, one of the pair of holding portions is provided with two solution injection portions into which a solution injection pin can be inserted and which are spaced apart from each other in the longitudinal direction.

According to a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding portions,
Since two solution injecting portions that are separated from each other in the longitudinal direction are formed, when the pin in which the two solution injecting portions are inserted is pulled out and then the portion in which the pin is inserted is closed, , For example, one solution injecting portion located distally from the sealed one end is inserted with a pretreatment liquid injection pin for injecting the pretreatment liquid, and the pretreatment liquid is injected into the hybridization bag, Hold the hybridization bag in the squeeze roller means,
By driving the drive mechanism engaged with the perforations formed in the pair of holding portions, the hybridization bag is moved with respect to the squeeze roller means, the pretreatment liquid is stirred, and the pretreatment is performed, Cut the hybridization bag between one solution injection part and the other solution injection part, drain the pretreatment liquid, and open the hybridization bag between the cutting part of the hybridization bag and the other solution injection part. After sealing, insert the hybridization solution injection pin into the other solution injection part, inject the hybridization solution into the hybridization bag, sandwich the hybridization bag with the squeeze roller means, and hold a pair of them. Drive the drive mechanism that engages the perforations formed on the Move the redidation bag to the squeeze roller means, stir the hybridization solution to perform pre-hybridization, and insert the probe solution injection pin into the other solution injection part to remove the probe solution The squeeze roller means is inserted into the hybridization bag, the squeeze roller means is sandwiched by the squeeze roller means, and the drive mechanism engaged with the perforations formed on the pair of holding portions is driven to move the hybridization bag to the squeeze roller means. Again, hybridization can be performed by moving and stirring the solution in which the probe solution was added to the hybridization solution,
Therefore, in addition to the pre-hybridization process and the hybridization process, it becomes possible to automate the pretreatment process as well, so that the experimenter does not vary the hybridization result, and the reproducibility of the hybridization is significantly improved. In addition to being able to improve it, it is possible to realize significant labor saving.

Further, according to a preferred embodiment of the present invention, one of the pair of holding portions is provided with two solution injecting portions into which the pins for injecting the solution can be inserted and which are separated from each other in the longitudinal direction. Even when a hole is formed in the portion where the pin is inserted after the two solution injecting parts pull out the inserted pin, for example, one solution injecting located distally from the sealed one end After inserting the hybridization solution injection pin into the part, inject the hybridization solution into the hybridization bag, seal the hybridization bag between one solution injection part and the other solution injection part, and squeeze roller. By sandwiching the hybridization bag in the means,
By driving the driving mechanism engaged with the perforations formed in the pair of holding portions, the hybridization bag is moved with respect to the squeeze roller means, the hybridization solution is stirred, and pre-hybridization is performed. After inserting the probe solution injection pin into the other solution injection part and injecting the probe solution into the hybridization bag, seal the hybridization bag between the other solution injection part and the sealed one end. Then, the squeeze roller means holds the hybridization bag, and the driving mechanism engaged with the perforations formed in the pair of holding portions is driven to move the hybridization bag with respect to the squeeze roller means. , The probe solution becomes the hybridization solution The added solution can be agitated to carry out the hybridization, thus allowing the hybridization process to be automated, so that the experimenter does not vary the hybridization results and The reproducibility can be greatly improved, and a large amount of labor can be saved.

In a preferred aspect of the present invention, a pin for injecting a solution is insertable into one of the pair of holding parts, and three solution injecting parts are formed spaced apart from each other in the longitudinal direction.

According to a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding portions,
Since three solution injection parts that are separated from each other in the longitudinal direction are formed, even when holes are formed in the part where the pins are inserted after the three solution injection parts are pulled out from the inserted pins, After inserting the pretreatment liquid injection pin into the first solution injection portion located at the most distal position from the sealed one end and injecting the pretreatment liquid into the hybridization bag, A perforation formed in a pair of holding parts by sealing the hybridization bag between the second solution injection part adjacent to the first solution injection part and sandwiching the hybridization bag by the squeeze roller means. Drive the driving mechanism that is engaged with the squeeze roller means to move the hybridization bag, stir the pretreatment liquid, perform the pretreatment, and The hybridization bag is cut between the part where the dization bag is sealed and the second solution injection part, the pretreatment liquid is discharged, and the part where the hybridization bag is cut and the second solution injection part Part, seal the hybridization bag, insert the hybridization solution injection pin into the second solution injection part, and inject the hybridization solution into the hybridization bag, and then the second solution injection part. And the third solution injecting part adjacent to the second solution injecting part, the hybridization bag is sealed, and the squeeze roller means holds the hybridization bag to form a pair of holding parts. Drive the drive mechanism that is engaged with the perforation to move the hybridization bag The pre-hybridization is performed by moving the probe solution to the scroller means, stirring the hybridization solution, and inserting the probe solution injection pin into the third solution injection part, and the probe solution is transferred into the hybridization bag. Then, the hybridization bag is sealed between the other solution injection part and the sealed one end, and the hybridization bag is sandwiched by the squeeze roller means, and the perforation formed on the pair of holding parts is performed. It is possible to carry out the hybridization by driving the driving mechanism which is engaged with the moving mechanism, moving the hybridization bag with respect to the squeeze roller means, and stirring the solution in which the probe solution is added to the hybridization solution. Yes, therefore prehybridization In addition to the process and the hybridization process, the pretreatment process can be automated, allowing the experimenter to significantly improve hybridization reproducibility without variation in hybridization results. In addition, it will be possible to realize significant labor savings.

[0024] In a further preferred aspect of the present invention, the plastic sheet is formed of a thermoplastic.

According to a further preferred embodiment of the present invention, since the plastic sheet is made of thermoplastic, the hybridization bag can be heat-sealed by using the heater, and the high-structure can be achieved with a simple structure. It will be possible to automate the hybridization process.

The above object of the present invention is also a hybridization bag formed of a plastic sheet and having one end sealed, wherein a pair of thick holding portions extending in the longitudinal direction is formed along both edges. At least one perforation is formed in each of the pair of holding portions along the longitudinal direction, and a pin for injecting a solution can be inserted into one of the pair of holding portions.
In the hybridization bag having one solution injection part, a plurality of absorptive regions containing a specific binding substance having a known structure or property are formed to be separated from each other, and the biochemical analysis unit is housed therein. The other end of the hybridization bag is sealed, the driving mechanism is engaged with the perforation formed in the pair of holding portions, and the hybridization bag is injected with the hybridization solution injection pin at the at least one solution injection portion. And injecting the hybridization solution into the hybridization bag, and inserting the hybridization solution injection pin into the at least 1
Take out from one solution injection part, and squeeze roller means,
The hybridization bag is sandwiched between the one end portion and the at least one solution injection unit, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means to move the hybridization bag. Pre-hybridization is carried out by stirring the hybridization solution in the hybridization bag, and the probe solution injection pin is inserted into the at least one solution injection part, and the probe solution contains a substance of biological origin labeled with a labeling substance. Is injected into the hybridization bag, the probe solution injection pin is pulled out from the at least one solution injection section, and the squeeze roller means is provided with the high pressure sensor between the one end and the at least one solution injection section. Hold the hybridization bag Driving the driving mechanism to move the hybridization bag with respect to the squeeze roller means, stirring the solution in which the probe solution is added to the hybridization solution in the hybridization bag, It is achieved by a hybridization method characterized by carrying out a hybridization.

According to the present invention, there is provided a hybridization bag formed of a plastic sheet and having one end sealed, wherein a pair of thick holding portions extending in the longitudinal direction is formed along both edges. In a pair of holding parts,
Each of the hybridization bags has a structure or a characteristic in which a perforation is formed along the longitudinal direction and at least one solution injection portion into which a solution injection pin can be inserted is formed in one of a pair of holding portions. A plurality of absorptive regions containing known specific binding substances are housed in biochemical analysis units formed separately from each other, the other end of the hybridization bag is sealed, and a pair of hybridization bags is formed. The driving mechanism is engaged with the perforation formed in the holding part, the hybridization solution injection pin is inserted into at least one solution injection part, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection is performed. The pin to at least one solution injection part The squeeze roller means is pulled out, and the hybridization bag between the one end and at least one solution injection section is clamped, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means, The hybridization solution in the hybridization bag is agitated to perform prehybridization, and the probe solution injection pin is inserted into at least one solution injection part to obtain a probe solution containing a substance derived from a living body labeled with a labeling substance. , Injecting into the hybridization bag, pulling out the probe solution injection pin from at least one solution injection part, and causing the squeeze roller means to sandwich the hybridization bag between one end and at least one solution injection part, and drive Drive mechanism, high Since the redidation bag is moved with respect to the squeeze roller means and the solution in which the probe solution is added to the hybridization solution in the hybridization bag is stirred, the hybridization is performed. , It is possible to automate the hybridization process, and therefore, the hybridization result does not vary depending on the experimenter, the hybridization reproducibility can be significantly improved, and the labor saving can be significantly reduced. Can be realized.

In a preferred embodiment of the present invention, the at least one solution injection part formed on one of the pair of holding parts of the hybridization bag,
After the insertion of the inserted hybridization solution injection pin and the inserted probe solution injection pin, the parts where the hybridization solution injection pin and the probe solution injection pin are inserted are closed.

According to a preferred embodiment of the present invention, at least one solution injection part formed on one of the pair of holding parts of the hybridization bag draws out the inserted hybridization solution injection pin and probe solution injection pin. After the injection, the hybridization solution injection pin and the probe solution injection pin are configured so that the inserted parts are closed, so after injecting the hybridization solution or the probe solution into the hybridization bag, hand,
It is not necessary to seal the hybridization bag, and the hybridization solution injection pin and the probe solution injection pin are respectively inserted into one solution injection part to insert the hybridization solution and the probe solution into the hybridization bag. Can be injected, thus simplifying the hybridization process.

In a further preferred aspect of the present invention, the at least one solution injection part formed in one of the pair of holding parts of the hybridization bag is formed of elastic rubber.

[0031] In a further preferred aspect of the present invention, after performing hybridization, the vicinity of the other end of the hybridization bag is cut, and the one end and the at least one solution are injected into the squeeze roller means. The hybridization bag between the parts, drive the drive mechanism, move the hybridization bag with respect to the squeeze roller means, to the hybridization solution,
It is configured to drain the solution to which the probe solution has been added.

[0032] In a preferred aspect of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding portions of the hybridization bag, and a first solution injecting portion and a first solution injecting portion which are separated from each other in the longitudinal direction. The plurality of absorptive regions containing a specific binding substance having a known structure or characteristic are housed in the hybridization bag having the solution injection part 2 formed therein to accommodate a biochemical analysis unit, The other end of the hybridization bag is sealed, and the hybridization bag is caused to engage the driving mechanism with the perforations formed in the pair of holding parts, and the first solution injection part and the second solution injection part. Second solution injection part of the solution injection part located distally from the one end of the hybridization bag , Inserting the hybridization solution injection pin, injecting the hybridization solution into the hybridization bag, removing the hybridization solution injection pin from the second solution injection part, and removing the hybridization bag from the The second solution injecting section is sealed near the one end side, the squeeze roller means holds the hybridization bag, and the driving mechanism is driven to move the hybridization bag to the squeeze roller means. , The hybridization solution in the hybridization bag is agitated, pre-hybridization is performed, and the probe solution injection pin is inserted into the first solution injection part to remove the probe solution. , The hybrid The probe solution injection pin is extracted from the first solution injection part, the hybridization bag is sealed near the one end side of the first solution injection part, and the squeeze is performed. A roller means holds the hybridization bag, drives the driving mechanism, and moves the hybridization bag with respect to the squeeze roller means so that the probe solution in the hybridization bag is a hybridization solution. The solution added to the agitator is agitated to prevent hybridization.

According to a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and the first solution injecting part and the second solution injecting part are longitudinally separated from each other. In the hybridization bag in which the solution injection part of is formed, a plurality of absorptive regions containing a specific binding substance having a known structure or property are accommodated in the biochemical analysis unit formed apart from each other,
The other end of the hybridization bag is sealed, the hybridization bag is engaged with the perforations formed in the pair of holding portions, and the first
Of the second solution injection part and the second solution injection part, which are located distally from one end of the hybridization bag.
The hybridization solution injection pin is inserted into the solution injection part of, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection pin is pulled out from the second solution injection part,
The hybridization bag is sealed in the vicinity of the one end of the second solution injecting section, and the squeeze roller means is sealed.
Hold the hybridization bag, drive the drive mechanism, move the hybridization bag with respect to the squeeze roller means, stir the hybridization solution in the hybridization bag, and execute pre-hybridization. The probe solution injection pin is inserted into the first solution injection part, the probe solution is injected into the hybridization bag, the probe solution injection pin is pulled out from the first solution injection part, and the hybridization bag is removed from the first solution injection part. In the vicinity of the one end side of the solution injecting part, the squeeze roller means is sandwiched with the hybridization bag, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means. The plug in the hybridization bag Since the probe solution is configured to stir the solution added to the hybridization solution to prevent hybridization, a hole is formed in the second solution injection portion by inserting the hybridization solution injection pin. By being formed and having the probe solution injection pin inserted therethrough, it is possible to automate the hybridization process even if a hole is formed in the first solution injection part, and therefore the result of the hybridization can be determined by the experimenter. It is possible to significantly improve the reproducibility of hybridization without variation and to realize a great labor saving.

In a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and the first solution injecting part and the first solution injecting part separated from each other in the longitudinal direction are provided. A biochemistry in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are formed in the hybridization bag in which the second solution injection part and the third solution injection part are formed, spaced apart from each other. The analysis unit is accommodated, the other end of the hybridization bag is sealed, and the hybridization bag is engaged with the drive mechanism to the perforations formed in the pair of holding portions, and the first Of the solution injection part, the second solution injection part, and the third solution injection part, the hybridization bag A pretreatment liquid injection pin is inserted into the third solution injection portion located farthest from the end, and the pretreatment liquid is injected into the hybridization bag. The third solution injecting section is pulled out, the hybridization bag is sealed in the vicinity of the one end side of the third solution injecting section, and the squeeze roller means holds the hybridization bag, A driving mechanism is driven, the hybridization bag is moved with respect to the squeeze roller means, the pretreatment liquid in the hybridization bag is stirred, and pretreatment is performed,
The hybridization bag is cut in the vicinity of the one end side of the third solution injection part to discharge the pretreatment liquid, and the hybridization solution injection pin is inserted into the second solution injection part. Then, the hybridization solution is injected into the hybridization bag, the hybridization solution injection pin is pulled out from the first solution injection section, and the hybridization bag is removed from the one end of the second solution injection section. In the vicinity of the section side, sealing is performed, the squeeze roller means holds the hybridization bag, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means. Stir the hybridization solution in the hybridization bag. , Pre-hybridization is performed, the probe solution injection pin is inserted into the first solution injection part, a probe solution is injected into the hybridization bag, and the probe solution injection pin is inserted into the first solution injection part. The hybridization bag is pulled out from the solution injecting section, the hybridization bag is sealed in the vicinity of the one end side of the first solution injecting section, the squeeze roller means holds the hybridization bag, and the driving mechanism is operated. By driving, the hybridization bag is moved with respect to the squeeze roller means, and the probe solution in the hybridization bag is agitated with the solution added to the hybridization solution so that the hybridization is not performed. It is configured.

According to a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding portions of the hybridization bag, and the first solution injecting portion and the second solution injecting portion are longitudinally spaced from each other. For biochemical analysis, a plurality of absorptive regions containing a specific binding substance having a known structure or property are formed in a hybridization bag in which the solution injection part and the third solution injection part of The unit is housed, the other end of the hybridization bag is sealed, and the hybridization bag is attached to the perforations formed on the pair of holding parts.
The drive mechanism is engaged to connect the first solution injection section, the second solution injection section, and the third solution injection section to the third solution injection section located farthest from one end of the hybridization bag. , The pretreatment liquid injection pin is inserted, the pretreatment liquid is injected into the hybridization bag, the pretreatment liquid injection pin is pulled out from the third solution injection part, and the hybridization bag is removed from the third solution injection part. In the vicinity of one end of the, the squeeze roller means is sandwiched, the hybridization bag is sandwiched, the driving mechanism is driven, the hybridization bag is moved with respect to the squeeze roller means, and The pretreatment solution is stirred to perform the pretreatment, and the hybridization bag is cut near the one end side of the third solution injection section. The pretreatment solution is discharged, the hybridization solution injection pin is inserted into the second solution injection part, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection pin is injected into the first solution. Of the second solution injecting section, the hybridization bag is sealed, the squeeze roller means holds the hybridization bag, the driving mechanism is driven, and the hybridization bag is removed. The squeeze roller means is moved to stir the hybridization solution in the hybridization bag, pre-hybridization is performed, and the probe solution injection pin is inserted into the first solution injection part to remove the probe solution. , In the hybridization bag Then, the probe solution injection pin is pulled out from the first solution injection part, the hybridization bag is sealed in the vicinity of one end side of the first solution injection part, and the squeeze roller means holds the hybridization bag. Then, the driving mechanism is driven to move the hybridization bag with respect to the squeeze roller means, and the solution in which the probe solution in the hybridization bag is added to the hybridization solution is stirred to carry out hybridization. Therefore, by inserting the pretreatment liquid injection pin, a hole is formed in the third solution injection portion, and by inserting the hybridization solution injection pin, a hole is formed in the second solution injection portion. Is formed, and by inserting the probe solution injection pin,
Even if holes are formed in the first solution injection part, it is possible to automate the hybridization process, so that the experimenter does not vary the hybridization result, and the reproducibility of hybridization is greatly improved. In addition to being able to improve it, it is possible to realize significant labor saving.

In a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and a first solution injecting part and a first solution injecting part which are separated from each other in the longitudinal direction. The plurality of absorptive regions containing a specific binding substance having a known structure or characteristic are housed in the hybridization bag having the solution injection part 2 formed therein to accommodate a biochemical analysis unit, The other end of the hybridization bag is sealed, and the hybridization bag is caused to engage the driving mechanism with the perforations formed in the pair of holding parts, and the first solution injection part and the second solution injection part. Among the solution injection parts, the second solution injection part located distally from the one end of the hybridization bag The pretreatment liquid injection pin is inserted, the pretreatment liquid is injected into the hybridization bag, the pretreatment liquid injection pin is pulled out from the second solution injection portion, and the squeeze roller means is connected to the one end portion. The hybridization bag is sandwiched between the second solution injecting sections, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means. The pretreatment liquid is stirred, pretreatment is performed, the hybridization bag is cut in the vicinity of the one end side of the second solution injection part, and the pretreatment liquid is discharged,
The hybridization solution injection pin is inserted into the first solution injection part, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection pin is pulled out from the first solution injection part. , The squeeze roller means,
The hybridization bag is sandwiched between the one end portion and the first solution injection unit, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means to move the hybridization bag. The hybridization solution in the hybridization bag is stirred, pre-hybridization is performed, the probe solution injection pin is inserted into the first solution injection part, and the probe solution is injected into the hybridization bag, The probe solution injection pin is pulled out from the first solution injection part, and the squeeze roller means holds the hybridization bag between the one end part and the first solution injection part, so that the drive mechanism is operated. Drive the hybridization bag into the squeeze roller hand Respect, is moved, by stirring a solution in which the probe solution was added to the hybridization solution in the hybridization bag, and is configured to cheat perform hybridization.

According to a preferred embodiment of the present invention, a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and the first solution injecting part and the second solution injecting part which are separated from each other in the longitudinal direction. In the hybridization bag in which the solution injection part of is formed, a plurality of absorptive regions containing a specific binding substance having a known structure or characteristic are accommodated in the biochemical analysis unit formed apart from each other,
The other end of the hybridization bag is sealed, the hybridization bag is engaged with the perforations formed in the pair of holding portions, and the first
Of the solution injecting section and the second solution injecting section, the second solution injecting section located distally from one end of the hybridization bag is inserted with the pretreatment solution injection pin, and the pretreatment solution is injected into the hybridization bag. And the pretreatment liquid injection pin is extracted from the second solution injection part, and the squeeze roller means holds the hybridization bag between the one end part and the second solution injection part to drive the drive mechanism. Then, the hybridization bag is moved with respect to the squeeze roller means to stir the pretreatment liquid in the hybridization bag to perform pretreatment, and the hybridization bag is attached to one end of the second solution injection part. In the vicinity of the section side, the pretreatment solution is cut off, the pretreatment solution is discharged, and the hybridization solution injection pin is inserted into the first solution injection section. The hybridization solution is injected into the hybridization bag, the hybridization solution injection pin is pulled out from the first solution injection section, and the squeeze roller means holds the hybridization bag between the one end and the first solution injection section. Then, the driving mechanism is driven, the hybridization bag is moved with respect to the squeeze roller means, and the hybridization solution in the hybridization bag is stirred,
Pre-hybridization is performed, the probe solution injection pin is inserted into the first solution injection part, the probe solution is injected into the hybridization bag, and the probe solution injection pin is pulled out from the first solution injection part, The squeeze roller means holds the hybridization bag between the one end and the first solution injecting section, drives the drive mechanism, and moves the hybridization bag with respect to the squeeze roller means, thereby forming a hybridization bag. In addition to pre-hybridization process and pre-hybridization process, it also automates the pretreatment process because the probe solution inside is configured to stir the solution added to the hybridization solution and not perform the hybridization. It will be possible Therefore, by the experimenter, without the hybridization results vary, with the hybridization reproducibility becomes possible to greatly improve, it is possible to realize significant labor saving.

[0038] In a further preferred aspect of the present invention, the at least one solution injection part formed in one of the pair of holding parts of the hybridization bag is inserted with the pretreatment liquid injection pin and the high treatment solution injection pin. After the hybridization solution injection pin and the probe solution injection pin are pulled out, the parts where the pretreatment liquid injection pin, the hybridization solution injection pin and the probe solution injection pin are inserted are closed.

According to a further preferred embodiment of the present invention, at least one solution injection part formed on one of the pair of holding parts of the hybridization bag has a pretreatment liquid injection pin and a hybridization solution injection pin inserted therein. And after pulling out the probe solution injection pin,
The pretreatment liquid injection pin, the hybridization solution injection pin, and the probe solution injection pin are configured so that the parts that are inserted therein are closed. Therefore, the pretreatment liquid, the hybridization solution, or the probe solution was injected into the hybridization bag. After that, it is not necessary to seal the hybridization bag prior to stirring the solution, and further, the hybridization solution injection pin and the probe solution injection pin are respectively inserted into the first solution injection part to insert the hybridization bag. It is possible to inject the hybridization solution and the probe solution into it, thus simplifying the hybridization process.

[0040] In a further preferred aspect of the present invention, the plastic sheet is formed of a thermoplastic and is configured to seal the hybridization bag by heat sealing.

According to a further preferred embodiment of the present invention, since the plastic sheet is made of thermoplastic resin and is configured to seal the hybridization bag by heat sealing, a heater for heat sealing is provided. It is sufficient to automate the hybridization process with a simple structure.

The object of the present invention is also to engage the perforations formed on a pair of thick-walled holding portions formed along both edges of the hybridization bag so that the hybridization bag can be moved in both forward and backward directions. A driving mechanism that can be transported to the hybridization bag, a hybridization solution injection pin configured to inject the hybridization bag solution into the hybridization bag, and a biological substance labeled with a labeling substance in the hybridization bag. A probe solution injection pin configured to inject a probe solution containing the heater, a sealing heater configured to heat seal the hybridization bag, a cutter for cutting the hybridization bag in the width direction, and the hybridization bag. A squeeze roller means configured to be held, and an insertion position at which the hybridization solution injection pin and the probe solution injection pin are inserted into the solution injection part formed in the pair of holding parts of the hybridization bag. And a retracted position separated from the solution injecting section.

According to the present invention, the hybridization device engages the perforations formed on the pair of thick-walled holding portions formed along both edges of the hybridization bag, and the hybridization bag is fixed. A drive mechanism that can be transported in both reverse directions, a hybridization solution injection pin configured to inject the hybridization bag solution into the hybridization bag, and a biological substance labeled with a labeling substance in the hybridization bag. The probe solution injection pin configured to inject the probe solution containing the heater, the sealing heater configured to heat seal the hybridization bag, the cutter for cutting the hybridization bag in the width direction, and the hybridization bag sandwiched. A squeeze roller means configured so that the hybridization solution injection pin and the probe solution injection pin are inserted into the solution injection part formed in the pair of holding parts of the hybridization bag, and the solution injection part. Since it is configured to be movable between the retracted position and the retracted position, a plurality of absorptive regions containing a specific binding substance having a known structure or characteristic are formed in the hybridization bag so as to be separated from each other. The hybridization bag can be mechanically and easily moved in the longitudinal direction by accommodating the biochemical analysis unit and engaging the drive mechanism with the perforations formed on the pair of thick-walled holding portions of the hybridization bag. Can be transported to and the hybridization solution injection pin is moved to the insertion position. Then, it is inserted into the solution injection part formed in the pair of holding parts, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection pin is retracted to the retracted position, and then by the heater for sealing. The hybridization bag is heat-sealed, the squeeze roller means holds the hybridization bag, the driving mechanism is driven, the hybridization bag is moved with respect to the squeeze roller means, and the hybridization solution is stirred, Pre-hybridization is performed, and then the probe solution injection pin is moved to the insertion position to be inserted into the solution injection part formed in the pair of holding parts, and the probe solution containing the substance of biological origin labeled with the labeling substance. The hybridization bag After injecting into the inside, retracting the probe solution injection pin to the retracted position, the sealing heater heat seals the hybridization bag, the squeeze roller means holds the hybridization bag, and drives the drive mechanism. Hybridization can be carried out by moving the hybridization bag relative to the squeeze roller means and agitating the solution to which the probe solution has been added to the hybridization solution, thus automating the hybridization process. Therefore, it is possible for the experimenter to significantly improve the reproducibility of the hybridization without variation in the hybridization result, and to realize the great labor saving. Become.

In a preferred embodiment of the present invention, the hybridization apparatus further comprises a pretreatment liquid injection pin for injecting a pretreatment liquid into the hybridization bag, and the pretreatment liquid injection pin is provided in the hybridization bag. The hybridization bag is configured to be movable between an insertion position inserted into the solution injection part formed in the pair of holding parts and a retracted position separated from the solution injection part.

According to a preferred embodiment of the present invention, the hybridization apparatus further comprises a pretreatment liquid injection pin for injecting the pretreatment liquid into the hybridization bag, and the pretreatment liquid injection pin is used for the hybridization bag. Prior to pre-hybridization, since it is configured to be movable between an insertion position that is inserted into the solution injection part formed in the pair of holding parts and a retracted position that is separated from the solution injection part, Move the treatment liquid injection pin to the insertion position and insert it into the solution injection part formed in the pair of holding parts, inject the pretreatment liquid into the hybridization bag, and retract the pretreatment liquid injection pin to the retracted position. After that, heat the hybridization bag with the heater for sealing and attach the hybridization to the squeeze roller means. The pretreatment can be performed by sandwiching the bag, driving the driving mechanism, moving the hybridization bag with respect to the squeeze roller means, and stirring the pretreatment liquid. In addition to the process and the prehybridization process, the pretreatment process can also be automated, thus allowing the experimenter to vary the hybridization results and greatly improve the reproducibility of the hybridization. In addition to being possible, it will be possible to realize significant labor savings.

In a preferred embodiment of the present invention, the substance derived from a living body is at least one selected from the group consisting of a radiolabeling substance, a fluorescent substance and a labeling substance which produces chemiluminescence by contacting with a chemiluminescent substrate. It is labeled with the labeling substance.

In the present invention, the fact that the substance derived from the living body is labeled with a fluorescent substance means that the substance is labeled with a fluorescent dye, and that the enzyme is bound to the labeled sample and then the enzyme is used as a fluorescent substrate. When the fluorescent substance is brought into contact with the fluorescent substance to emit fluorescence, the fluorescent substance is labeled with the obtained fluorescent substance.

[0048] In a preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of holes formed therein, and the plurality of absorptive regions are absorptive to the plurality of holes of the substrate. The material is filled and formed.

According to a preferred embodiment of the present invention, the biochemical analysis unit is provided with a substrate having a plurality of holes formed therein, and a plurality of absorptive regions are filled in the plurality of holes of the substrate with an absorptive material. Since it is formed by being formed, the distance between the adsorbing regions adjacent to each other can be controlled as desired, and therefore, the specific binding contained in the adsorbing regions of the biochemical analysis unit can be controlled by the hybridization device. After selectively hybridizing a substance of biological origin labeled with a radioactive labeling substance, the stimulable phosphor layer formed on the stimulable phosphor sheet is adsorbed by the biochemical analysis unit. The stimulable phosphor layer of the stimulable phosphor sheet by scanning with excitation light, and the stimulable phosphor is excited, and the emitted stimulable light is photoelectrically converted. Detected By Rukoto, with high resolution, it is possible to produce biochemical analysis data having an excellent quantitative characteristic.

In a further preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of through holes formed therein, and the plurality of absorptive regions are provided in the plurality of through holes of the substrate. , Is formed by being filled with an adsorptive material.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed by press-fitting an absorptive film containing an absorptive material into the plurality of through holes of the substrate.

In another preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of recesses formed therein, and the plurality of absorptive regions are provided in the plurality of recesses of the substrate. The adsorbent material is filled and formed.

[0053] In another preferred aspect of the present invention, the biochemical analysis unit includes an absorptive substrate formed of an absorptive material, and at least one surface of the absorptive substrate attenuates radiation. A substrate having a plurality of through-holes having a property is adhered, and the specific binding substance is contained in the absorptive substrate in the plurality of through-holes formed on the substrate, thereby adsorbing the plurality of adsorbents. A sex region is formed.

According to another preferred embodiment of the invention,
The biochemical analysis unit has an absorptive substrate formed of an absorptive material, and has a property of attenuating radiation on at least one surface of the absorptive substrate and closely adhering the substrate with a plurality of through holes formed therein. Since a plurality of absorptive regions are formed by containing a specific binding substance in the absorptive substrate in the plurality of through-holes formed in the substrate, the intervals between adjacent absorptive regions can be set as desired. Therefore, the specific binding substance contained in the adsorptive region of the biochemical analysis unit is selectively changed from the biologically-derived substance labeled with the radiolabeled substance by the hybridization device, After hybridizing, the stimulable phosphor layer formed on the stimulable phosphor sheet is exposed to a radioactive labeling substance contained in the absorptive region of the biochemical analysis unit, and the stimulable phosphor layer is exposed. The stimulable phosphor layer of the body sheet is scanned with excitation light, the stimulable phosphor is excited, and the emitted stimulable light is photoelectrically detected to provide high resolution and excellent quantitativeness. It becomes possible to generate data for biochemical analysis.

In a preferred aspect of the present invention, the substrate of the biochemical analysis unit has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating radiation, a plurality of absorptive regions are densely arranged in the biochemical analysis unit. Even when formed, a specific binding substance that can specifically bind to a substance of biological origin in a plurality of absorptive regions of the biochemical analysis unit and has a known base sequence, base length, composition, etc. To the specific binding substance adsorbed to the plurality of adsorptive regions, the substance of biological origin labeled with a radiolabeling substance is specifically bound, and selectively labeled. When exposing the stimulable phosphor layer of the stimulable phosphor sheet by stacking the chemical analysis unit and the stimulable phosphor sheet and using the radiolabeled substance selectively contained in the plurality of absorptive regions Included in each adsorptive area The electron beam (β-ray) emitted from the radioactive labeling substance present in the biochemical analysis unit can be effectively prevented from scattering within the substrate of the biochemical analysis unit. The electron beam (β-ray) emitted from the labeling substance may be selectively incident on the region of the corresponding photostimulable phosphor layer to expose only the region of the corresponding photostimulable phosphor layer. Since it becomes possible, the photostimulable phosphor layer exposed by the radiolabeled substance is scanned with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected to obtain high resolution. , It becomes possible to generate data for biochemical analysis with excellent quantification.

In a preferred aspect of the present invention, when the substrate of the biochemical analysis unit has a distance equal to the distance between the adsorbing regions adjacent to each other, the radiation is transmitted through the substrate. It has the property of attenuating energy to 1/5 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has a distance equal to the distance between the adjacent absorptive regions, the radiation has passed through the substrate, the radiation Energy of 1 /
It has the property of being attenuated to 10 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has a radiation equal to the distance between the adsorbing regions adjacent to each other, the radiation is transmitted through the substrate. Energy of 1 /
It has the property of being attenuated to 50 or less.

[0060] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to the distance between the adjacent absorptive regions, the radiation is Energy of 1 /
It has the property of being attenuated to 100 or less.

[0061] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit is exposed to radiation by a distance equal to the distance between the adjacent absorptive regions, the radiation is absorbed. Energy of 1 /
It has the property of being attenuated to 500 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has a distance equal to the distance between the adjacent absorptive regions, the radiation has passed through the substrate, the radiation Energy of 1 /
It has the property of being attenuated to 1000 or less.

In the present invention, the material for forming the substrate of the biochemical analysis unit preferably has a property of attenuating radiation, but is not particularly limited, and it is an inorganic compound material or an organic compound material. Any of the compound materials can be used, and metallic materials, ceramic materials or plastic materials are preferably used.

In the present invention, examples of the inorganic compound material that can be used to form the substrate of the biochemical analysis unit include gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, Metals such as nickel, cobalt, lead, tin and selenium; brass, stainless steel,
Alloys such as bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride;
Examples thereof include metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; and inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material that can be used to form the substrate of the biochemical analysis unit, and as the polymer compound that can be preferably used, For example, polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethylmethacrylate and butylacrylate / methylmethacrylate copolymers;
Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon 4,10 Nylon such as; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, Cellulose acetate, Nitrocellulose, Starch, Alginic acid Polysaccharides such as calcium and hydroxypropyl methylcellulose; chitin; chitosan; sumacum; gelatin, collagen, kerula Polyamides such emissions and the like can be mentioned copolymers of these polymeric compounds.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the substrate of the biochemical analysis unit is preferably formed of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more, Specific gravity is 1.5g / c
It is particularly preferable that it is formed of a compound material or a composite material of m ³ or more and 23 g / cm ³ or less.

[0067] In another preferred aspect of the present invention, the biochemical analysis unit includes an absorptive substrate formed of an absorptive material, and the plurality of absorptive regions are provided on the absorptive substrate. Alternatively, it is formed by dropping a solution containing a specific binding substance having a known property.

In a preferred embodiment of the present invention, 10 or more absorptive regions are formed on the substrate or the absorptive substrate of the biochemical analysis unit.

In a further preferred aspect of the present invention, 50 or more absorptive regions are formed on the substrate or the absorptive substrate of the biochemical analysis unit.

[0070] In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 100 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 500 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 1000 or more absorptive regions.

[0073] In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 5000 or more absorptive regions.

[0074] In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 10,000 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit is formed with 50,000 or more absorptive regions.

In a further preferred aspect of the present invention, 100,000 or more absorptive regions are formed on the substrate or the absorptive substrate of the biochemical analysis unit.

In a preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit is
It has a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit has a size of less than 1 mm 2. ing.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit has a size of less than 0.5 mm 2. Have

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit has a size of less than 0.1 mm 2. Have

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit has a size of less than 0.5 mm 2. Have

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed on the substrate or the absorptive substrate of the biochemical analysis unit has a size of less than 0.01 mm 2. Have

In a preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 10 or more per cm 2 on the substrate or the absorptive substrate of the biochemical analysis unit.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 50 or more per square centimeter on the substrate or the absorptive substrate of the biochemical analysis unit.

[0085] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in a density of 100 or more per cm 2 on the substrate or the absorptive substrate of the biochemical analysis unit.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 500 or more per cm 2 on the substrate or the absorptive substrate of the biochemical analysis unit.

In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit has a plurality of the absorptive regions of 1000 /
It is formed with a density of not less than square centimeters.

In a further preferred aspect of the present invention, the substrate or the absorptive substrate of the biochemical analysis unit has the plurality of absorptive regions of 5000 /
It is formed with a density of not less than square centimeters.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 10,000 or more per cm 2 on the substrate or the absorptive substrate of the biochemical analysis unit.

In a preferred aspect of the present invention, the plurality of absorptive regions are formed in a regular pattern on the substrate or the absorptive substrate of the biochemical analysis unit.

[0091] In a preferred aspect of the present invention, the plurality of absorptive regions are formed in a substantially circular shape on the substrate or the absorptive substrate of the biochemical analysis unit.

In another preferred aspect of the present invention, the plurality of absorptive regions are formed in a substantially rectangular shape on the substrate or the absorptive substrate of the biochemical analysis unit.

In the present invention, a porous material or a fibrous material is preferably used as the absorptive material for forming the absorptive region or the absorptive substrate of the biochemical analysis unit. The absorptive region or the absorptive substrate can be formed by using the porous material and the fiber material together.

In the present invention, the porous material used to form the adsorptive region of the biochemical analysis unit or the adsorptive substrate may be either an organic material or an inorganic material, or an organic / inorganic composite. .

In the present invention, the organic porous material used to form the adsorptive region or the adsorptive substrate of the biochemical analysis unit is not particularly limited, but is a carbon material such as activated carbon or a membrane. A material capable of forming a filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6, nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof. .

In the present invention, the inorganic porous material used for forming the absorptive region or the absorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, platinum, Examples thereof include metals such as gold, iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fibrous material used for forming the adsorptive region or the adsorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, nylon 6 or nylon. Nylons such as 6,6 and nylon 4,10, nitrocellulose,
Examples thereof include cellulose derivatives such as cellulose acetate and cellulose butyrate.

In the present invention, the absorptive region of the biochemical analysis unit is subjected to oxidation treatment such as electrolytic treatment, plasma treatment and arc discharge; primer treatment using silane coupling agent, titanium coupling agent and the like; surfactant. It can also be formed by surface treatment such as treatment.

[0099]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit carrying a specific binding substance to which a substance of biological origin is hybridized by the hybridization apparatus according to a preferred embodiment of the present invention.

As shown in FIG. 1, the biochemical analysis unit 1 according to this embodiment includes a substrate 2 formed of aluminum and having a large number of substantially circular through holes 3 formed therein at a high density. Nylon 6 is filled inside the large number of through holes 3, and a large number of dot-shaped absorptive regions 4 are formed.
Are formed.

Although not shown exactly in FIG. 1, in the present embodiment, approximately 500 through-holes 3 of approximately circular shape having a size of approximately 10,000 square millimeters of approximately 10,000 are provided.
Substrate 2 regularly with a density of 0 pieces / cm 2.
Is formed in. The absorptive region 4 has a large number of through holes 3 so that its surface is located at the same height as the surface of the substrate 2.
Nylon 6 is filled inside and formed.

FIG. 2 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 2, in a large number of absorptive regions 4 regularly formed in the biochemical analysis unit 1, for example, as a specific binding substance, the base sequence is Multiple known different cDNAs
Are dropped using a spotting device.

As shown in FIG. 2, the spotting device comprises an injector 5 and a CCD camera 6 for injecting a solution of a specific binding substance toward the biochemical analysis unit 1.
Has a spotting head with a CCD camera 6
While observing the tip of the injector 5 and the through hole 3 into which the cDNA should be dropped, when the tip of the injector 5 and the center of the through hole 3 into which the cDNA is dropped match, The cDNA is configured so as to be dropped, and it is guaranteed that the cDNA can be accurately dropped onto the absorptive region 4 formed in the large number of through holes 3.

FIG. 3 is a schematic plan view of a hybridization bag according to a preferred embodiment of the present invention.

As shown in FIG. 3, the hybridization bag 7 according to the present embodiment is formed by plastic sheets 8 and 8 having thermoplasticity. As shown in FIG. 3, both ends of the plastic sheets 8 and 8 are formed. The region near the part is sealed and closed to form a seal part 8a.

As shown in FIG. 3, both edges of the plastic sheets 8 and 8 are formed thick, and a pair of holding portions for holding the hybridization bag 7 are provided at both edges of the hybridization bag 7. The parts 9 are formed, and the pair of holding parts 9 are provided with the perforations 10 along the longitudinal direction.

Further, as shown in FIG. 3, holding portions 9 formed on both edges of the hybridization bag 7.
On one side, a cylindrical first solution injection part 11a and a columnar solution injection part 11b are formed so as to cross the holding part 9, respectively. The first solution injecting portion 11a and the second solution injecting portion 11b are each formed of elastic rubber that is compressed in the radial direction so that the pin can be inserted while the pin is pulled out when the pin is pulled out. It is formed so that the hole formed when it is inserted is closed, and the second solution injecting portion 11b has the sealing portion 8a.
Is formed at a position farther from the first solution injection portion 11a.
Is formed at a position closer to the seal portion 8a.

[0110] For hybridization, the hybridization bag 7 constructed as described above is used.
A biochemical analysis unit 1 in which a specific binding substance having a known structure or characteristic is dropped in a large number of absorptive regions 4 is accommodated therein, and the end portion on the opposite side to the end portion on which the seal portion 8a is formed. Are sealed to form a seal portion 8b.

FIG. 4 is a schematic plan view of a hybridization device according to a preferred embodiment of the present invention.

As shown in FIG. 4, the hybridization apparatus according to the present embodiment has two sets of endless belts 12 and 12 vertically facing each other on both sides thereof.
Is equipped with. Although not shown in FIG. 4, each endless belt 12 has a surface formed with a claw that engages with the perforations 10 formed on the pair of holding portions 9 of the hybridization bag 7.

As shown in FIG. 4, the hybridization apparatus according to this embodiment includes a cutter 13 for cutting the hybridization bag 7 and a heater 14 for heating and sealing the hybridization bag 7.
And a pair of upper and lower squeeze rollers 15.

As shown in FIG. 4, the hybridization apparatus according to the present embodiment is further communicated with a pretreatment liquid tank (not shown) for containing the pretreatment liquid, and the A pretreatment liquid pin 16 for injecting the treatment liquid and a hybridization solution tank (not shown) for accommodating the hybridization solution are communicated with each other, and a hybridization solution injection pin 17 for injecting the hybridization solution into the hybridization bag 7. And a probe solution injection pin 18 for injecting the probe solution into the hybridization bag 7, which communicates with a probe solution chip (not shown) containing a probe solution containing a substance of biological origin labeled with a labeling substance. , Pretreatment pin 16, hybridization solution injection Pin 17 and the probe solution injection pin 18 is mounted on the injection pin head 19.

FIG. 5 is a block diagram showing the control system, drive system and input system of the hybridization apparatus according to the preferred embodiment of the present invention.

As shown in FIG. 5, the control system of the hybridization apparatus according to this embodiment includes a control unit 20 for controlling the operation of the entire hybridization apparatus, and the drive system of the hybridization apparatus is the endless belt 12 Endless belt motor 21 that drives the endless belt 1 and the pin head 19 in a direction parallel to the driving direction of the endless belt 12 and the endless belt 1.
The pre-treatment liquid pump 2 that supplies the pre-treatment liquid to the pre-treatment injection pin 16 from the pin head motor 22 that is moved in the direction orthogonal to the driving direction of 2 and the pre-treatment liquid tank (not shown).
3a, a hybridization solution pump 23b for supplying the hybridization solution to the hybridization solution injection pin 17 from a hybridization solution tank (not shown), and a probe solution pin from the probe solution chip (not shown). 18, a probe solution pump 23c for supplying to 18, a cutter 13 for cutting the hybridization bag 7, and a heater 1 for heating and sealing the hybridization bag 7.
It is equipped with 4.

As shown in FIG. 5, the input system of the hybridization apparatus according to this embodiment has a keyboard 24.

The hybridization apparatus according to this embodiment having the above-described configuration is as follows.
The specific binding substance contained in the large number of adsorptive regions 4 of the biochemical analysis unit 1 is selectively hybridized with the substance of biological origin labeled with the labeling substance contained in the probe solution.

First, the user holds a pair of holding portions 9
Respectively, a pair of upper and lower endless drums 12, 12
The perforation 10 formed between the pair of holding portions 9 is sandwiched between the pair of upper and lower endless drums 12, 1
The hybridization bag 7 is set in the hybridization device so as to be engaged with the second claw (not shown), and the start signal is input to the keyboard 24.

The start signal is the control unit 2
5, the control unit 25 receives the start signal and outputs a drive signal to the endless belt motor 26 to drive the endless belts 12, 12 in the forward direction indicated by arrow A in FIG.

As a result, the perforations 1 formed on the pair of holding portions 9 of the hybridization bag 7
Since the claws (not shown) of the endless drums 12 and 12 are engaged with the hybridization bag 7
Are moved in the positive direction indicated by arrow A in FIG.

The hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the second solution injection portion 11b formed in the holding portion 9 of the hybridization bag 7 reaches the position facing the pin head 19. Then,
A drive stop signal is output from the control unit 20 to the endless belt motor 21, and the endless belts 12, 12 are stopped.

Then, the control unit 20 outputs a pretreatment liquid injection pin drive signal to the pin head motor 22 so that the pretreatment liquid injection pin 16 held by the pin head 19 is held in the holding portion 9 of the hybridization bag 7. The pin head 19 is attached to the endless belt 1 so as to be located at a position opposite to the formed second solution injection part 11b.
2 is moved in a direction parallel to the driving direction of the endless belt 12, and then the pretreatment liquid injection pin 16 is formed in the holding portion 9 of the hybridization bag 7 in the direction perpendicular to the driving direction of the endless belt 12. The pin head 19 is moved until it is inserted into the injection portion 11b.

Thus, when the inside of the hybridization bag 7 communicates with the pretreatment liquid tank (not shown) via the pretreatment liquid pin 16, the control unit 20 sends a drive stop signal to the pin head motor 22. Then, the pin head 19 is stopped, and a drive signal is further output to the pretreatment liquid pump 23a so that the pretreatment liquid contained in the pretreatment liquid tank is driven to a high level via the pretreatment liquid injection pin 16. It is injected into the hybridization bag 7.

When a predetermined amount of pretreatment liquid has been injected into the hybridization bag 7 after a predetermined time has elapsed, the control unit 20 causes the pretreatment liquid pump 2 to
A drive stop signal is output to 3a to stop the supply of the pretreatment liquid, and then a retract signal is output to the pin head motor 22 to return the pin head 19 to the original position.

When the pretreatment liquid injection pin 16 is pulled out,
The hole formed in the second solution injection portion 11b is closed by the elasticity of the compressed rubber.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the reverse direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 4, and the sealing portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the pair of squeeze rollers 15 stir the pretreatment liquid injected into the hybridization bag 7, and the pretreatment liquid is stored in the hybridization bag 7. Pretreatment is performed by uniformly contacting the absorptive region 4 of the chemical analysis unit 1.

Thus, the second solution injection part 11b formed in the holding part 9 of the hybridization bag 7 is
When reaching just before the pair of squeeze rollers 15, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12, and then outputs a drive signal to the endless belt motor 21. The output is performed to drive the endless belt motor 21 in the forward direction indicated by arrow A in FIG.

As a result, the pair of squeeze rollers 15 agitate the pretreatment liquid injected into the hybridization bag 7, and the pretreatment liquid is stored in the hybridization bag 7 and is used as the biochemical analysis unit 1. The uniform contact with the absorptive region 4 of No. 3 and the pretreatment is promoted.

In this way, the pretreatment liquid contained between the second solution injecting portion 11b and the sealing portion 8a of the hybridization bag 7 is agitated by the pair of squeeze rollers 15, and the pretreatment liquid is hybridized. The biochemical analysis unit 1 housed in the bag 7 is uniformly contacted with the absorptive region 4 and the pretreatment proceeds.

When the above operation is repeated for a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. The drive signal is output to the belt motor 21, and the second solution injection portion 11b of the hybridization bag 7 is output.
However, until it passes the cutter 13, the endless belt 1
2 and 12 are driven.

When the second solution injecting portion 11b of the hybridization bag 7 passes through the cutter 13, the control unit 20 outputs a drive stop signal to the endless belt motor 21 and the endless belts 12, 12 are fed.
Drive is stopped, and then a drive signal is output to the cutter 13, so that the second solution injection part 11b and the first solution injection part 1
Between the perforation 10 between 1a and the second solution injection part 11b, the hybridization bag 7 is cut to separate the second solution injection part 11b, and the opening (FIG. (Not shown).

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the reverse direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction indicated by the arrow B in FIG. 4, and the sealing portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the pretreatment liquid injected into the inside of the hybridization bag 7 by the pair of squeeze rollers 15 is cut by the cutter 13 of the hybridization bag 7 to form an opening portion. It is squeezed out via (not shown).

The squeezed pretreatment liquid is recovered in a pretreatment liquid recovery tank (not shown) arranged below the endless belts 12, 12.

Next, the control unit 20 outputs a drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the forward direction indicated by arrow A in FIG.

As a result, the hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the cutter 1 is moved.
The opening formed by being cut by 3 is the heater 1
When it reaches the position facing 4, the control unit 2
0 outputs a driving stop signal to the endless belt motor 21, stops driving the endless belts 12 and 12, turns on the heater 14, and heat seals the opening formed in the hybridization bag 7.

Then, the control unit 20 outputs a drive signal to the endless belt motor 21 to drive the endless belts 12 and 12 in the forward direction indicated by the arrow A in FIG. 4 to hold the hybridization bag 7. First solution injection part 11a formed in part 9
However, when it reaches a position facing the pin head 19, the endless belt motor 21
The drive stop signal is output to the endless belt 1
2, 12 are stopped.

Next, the control unit 20 outputs a hybridization solution injection pin drive signal to the pin head motor 22, and the hybridization solution injection pin 17 held by the pin head 19 is formed in the holding portion 9 of the hybridization bag 7. The pin head 19 is moved in a direction parallel to the driving direction of the endless belt 12 so as to be located at a position facing the first solution injecting portion 11 a, and then in a direction orthogonal to the driving direction of the endless belt 12. The pin head 19 is moved until the hybridization solution injection pin 17 is inserted into the first solution injection portion 11a formed in the holding portion 9 of the hybridization bag 7.

Thus, the hybridization bag 7 is inserted through the hybridization solution injection pin 17.
When the inside of the container is communicated with a hybridization solution tank (not shown), the control unit 20 outputs a drive stop signal to the pin head motor 22 to stop the pin head 19, and further to the hybridization solution pump 23b. A drive signal is output to inject the hybridization solution contained in the hybridization solution tank into the hybridization bag 7 via the hybridization solution injection pin 17.

When a predetermined amount of hybridization solution has been injected into the hybridization bag 7 after a predetermined time has elapsed, the control unit 20
Outputs a drive stop signal to the hybridization solution pump 23b to stop the supply of the hybridization solution, and then outputs a retract signal to the pin head motor 22 to return the pin head 19 to the original position.

Hybridization solution injection pin 17
When is pulled out, the hole formed in the first solution injecting portion 11a is closed by the elasticity of the compressed rubber.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the reverse direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 4, and the sealing portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the pair of squeeze rollers 15 stir the hybridization solution injected into the hybridization bag 7, and the hybridization solution is stored in the hybridization bag 7. Pre-hybridization is performed by making uniform contact with the adsorptive region 4 of the chemical analysis unit 1.

Thus, the first solution injecting portion 11a formed in the holding portion 9 of the hybridization bag 7 is
When reaching just before the pair of squeeze rollers 15, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12, and then outputs a drive signal to the endless belt motor 21. The output is performed to drive the endless belt motor 21 in the forward direction indicated by arrow A in FIG.

As a result, the pair of squeeze rollers 15 agitate the hybridization solution injected into the hybridization bag 7, and the hybridization solution is stored in the hybridization bag 7. The uniform contact with the absorptive region 4 of No. 1 promotes prehybridization.

In this way, the hybridization solution contained between the first solution injecting part 11a and the sealing part 8a of the hybridization bag 7 is agitated by the pair of squeeze rollers 15, and the hybridization solution is hybridized. Pre-hybridization proceeds by uniformly contacting the absorptive region 4 of the biochemical analysis unit 1 housed in the bag 7.

When the above operation is repeated a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. A drive signal is output to the belt motor 21 to drive the endless belts 12, 12.

As a result, the hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the first solution injection portion 11a formed in the holding portion 9 of the hybridization bag 7 faces the pin head 19. When the position is reached, a drive stop signal is output from the control unit 20 to the endless belt motor 21, and the endless belts 12, 12 are stopped.

Next, the control unit 20 sends the probe solution injection pin drive signal to the pin head motor 22.
To the probe solution injection pin 18 held by the pin head 19 so that the probe solution injection pin 18 is positioned at a position facing the first solution injection part 11a formed in the holding part 9 of the hybridization bag 7. The endless belt 12 is moved in a direction parallel to the driving direction, and then
The probe solution injection pin 17 is formed in the holding part 9 of the hybridization bag 7 in the direction orthogonal to the driving direction of the endless belt 12 and is formed into the first solution injection part 11a.
The pin head 19 is moved until it is inserted inside.

In this way, when the inside of the hybridization bag 7 is communicated with the probe solution solution tank (not shown) via the probe solution injection pin 18, the control unit 20 sends a drive stop signal to the pin head motor 22. Output, stop the pin head 19, and further output a drive signal to the probe solution pump 23c,
The probe solution stored in the probe solution tank,
It is injected into the inside of the hybridization bag 7 through the probe solution injection pin 18.

When a predetermined amount of probe solution has been injected into the hybridization bag 7 after a predetermined time has elapsed, the control unit 20 outputs a drive stop signal to the probe solution pump 23c to output the probe solution. After stopping the supply of the pin head, a retract signal is output to the pin head motor 22 to return the pin head 19 to the original position.

When the probe solution injection pin 18 is pulled out, the hole formed in the first solution injection portion 11a is closed by the elasticity of the compressed rubber.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the reverse direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction indicated by the arrow B in FIG. 4, and the sealing portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the solution in which the probe solution is added to the hybridization solution injected into the inside of the hybridization bag 7 is stirred by the pair of squeeze rollers 15, and the probe solution is added to the hybridization solution. The solution to which is added uniformly contacts the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7,
Hybridization is performed.

Thus, the first solution injection part 11a formed in the holding part 9 of the hybridization bag 7 is
When reaching just before the pair of squeeze rollers 15, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12, and then outputs a drive signal to the endless belt motor 21. The output is performed to drive the endless belt motor 21 in the forward direction indicated by arrow A in FIG.

As a result, the solution in which the probe solution was injected into the hybridization solution inside the hybridization bag 7 was agitated by the pair of squeeze rollers 15, and the solution to which the probe solution was added was mixed with the solution. The absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7 is evenly contacted, and the hybridization is promoted.

In this way, the solution in which the probe solution has been injected into the hybridization solution contained between the first solution injection section 11a and the sealing section 8a of the hybridization bag 7 is agitated by the pair of squeeze rollers 15. Then, the solution obtained by adding the probe solution to the hybridization solution uniformly contacts the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7, and the hybridization proceeds.

When the above operation is repeated a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. The drive signal is output to the belt motor 21, and the first solution injection portion 11a of the hybridization bag 7 is output.
However, until it passes the cutter 13, the endless belt 1
2 and 12 are driven.

When the first solution injecting portion 11a of the hybridization bag 7 passes through the cutter 13, the control unit 20 outputs a drive stop signal to the endless belt motor 21 so that the endless belts 12 and 12 can be driven.
Is stopped, and then a drive signal is output to the cutter 13, so that the perforation 10 located at the seal portion 8a of the first solution injecting portion 11a and the first solution injecting portion 11a.
The hybridization bag 7 is cut between it and a to separate the first solution injection part 11a, and an opening (not shown) is formed in the hybridization bag 7.
To form.

Then, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the reverse direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction indicated by the arrow B in FIG. 4, and the sealing portion 8a of the hybridization bag 7 is moved.
However, when passing through the pair of squeeze rollers 15, the probe solution is injected into the hybridization solution contained in the hybridization bag 7 by the pair of squeeze rollers 15, and the hybridization bag 7 is cut by the cutter 13. And is squeezed through the formed opening (not shown).

The solution prepared by injecting the probe solution into the squeezed hybridization solution is recovered in a hybridization solution recovery tank (not shown) arranged below the endless belts 12, 12.

When the solution in which the probe solution is added to the hybridization solution is discharged from the hybridization bag 7, the hybridization bag 7
Are collected from the hybridization apparatus, the biochemical analysis unit 1 is taken out from the hybridization bag 7, and washed in the container containing the cleaning solution.

Thus, the radiation data of the radioactive labeling substance as the labeling substance and the fluorescence data of the fluorescent substance such as the fluorescent dye are recorded in a large number of the absorptive regions 4 of the biochemical analysis unit 1. The fluorescence data recorded in the absorptive region 4 is read by a scanner described later, and biochemical analysis data is generated.

On the other hand, the radiation data of the radiolabeled substance is
The radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by a scanner described later to generate biochemical analysis data.

FIG. 6 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 6, the stimulable phosphor sheet 25 according to this embodiment has a large number of substantially circular through holes 2.
8 is provided with a nickel support 26 formed regularly, and a stimulable phosphor is embedded in a large number of through-holes 28 formed in the support 16 to provide a large number of stimulable phosphor layers. The area 27 is formed in a dot shape.

The large number of through holes 23 are formed in the support 26 in the same pattern as the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and each stimulable phosphor layer region. 27 is formed to have the same size as the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1.

Therefore, although not shown exactly in FIG. 6, there is about 5 of the substantially circular stimulable phosphor layer region 27 having a size of about 10,000 square millimeters of about 10,000.
The support 26 of the stimulable phosphor sheet 25 has a density of 000 pieces / square centimeter and the same regular pattern as the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, It is formed in a dot shape.

In the present embodiment, the support 26
Of the through hole 2 formed in the support 26 such that the surface of the support and the surface of the stimulable phosphor layer region 27 are located at the same height.
A stimulable phosphor is embedded in 8 to form a stimulable phosphor sheet 25.

FIG. 7 shows a large number of stimulable phosphor layers formed on the stimulable phosphor sheet 25 by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1. It is a schematic sectional drawing which shows the method of exposing the area | region 27.

In the present embodiment, the biochemical analysis unit 1 is formed by filling nylon 6 into a large number of through holes 3 formed in the substrate 2 made of aluminum. Even when subjected to a treatment with a liquid, it hardly expands or contracts, and therefore each of the large number of absorptive regions 4 formed in the biochemical analysis unit 1 corresponds to the stimulable phosphor sheet 25. The stimulable phosphor layer region 27 can be exposed by superimposing the stimulable phosphor sheet 25 and the biochemical analysis unit 1 so as to face the stimulable phosphor layer region 27 accurately. become.

Thus, over a predetermined time, each of the large number of absorptive regions 4 formed in the biochemical analysis unit 1 corresponds to the stimulable phosphor layer region 27 formed in the stimulable phosphor sheet 25. By stacking the biochemical analysis unit 1 and the stimulable phosphor sheet 25 so as to face each other, a large number of the stimulable phosphor sheet 25 formed by the radioactive labeling substance contained in the absorptive region 4 is formed. The photostimulable phosphor layer region 27 of is exposed.

At this time, an electron beam (β-ray) is emitted from the radiolabeled substance adsorbed in the absorptive region 4, but the absorptive region 4 of the biochemical analysis unit 1 has a property of attenuating radiation. Since the substrate 2 made of aluminum is formed in a dot shape with being separated from each other, the electron beam (β-ray) emitted from each absorptive region 4 is
The photostimulable phosphor layer scattered in the substrate 2 of the biochemical analysis unit 1 and mixed with the electron beam (β-ray) emitted from the adsorbing regions 4 adjacent to each other and facing the adsorbing regions 4 adjacent to each other. The stimulable phosphor layer region 27 of the stimulable phosphor sheet 25 can be effectively prevented from entering the region 27, and further, the stimulable phosphor layer region 27 is provided on the nickel support 26 having a property of attenuating radiation. Within the large number of through holes 28 formed,
Since the stimulable phosphor is embedded and formed, the electron beam (β-ray) emitted from each absorptive region 4 is scattered in the support 26 of the stimulable phosphor sheet 25 and faces each other. The stimulable phosphor layer region 2 adjacent to the stimulable phosphor layer region 27
It becomes possible to effectively prevent the electron beam (β-ray) emitted from the radiolabeled substance contained in the absorptive region 4 from facing the absorptive region 4. The electron beam (β-ray) emitted from the radiolabeled substance contained in the absorptive region 4 can be selectively made incident on the stimulable phosphor layer region 27 which is adjacent to the absorptive region 4. It is possible to reliably prevent the photostimulable phosphor from being exposed by being incident on the photostimulable phosphor layer region 27 to be exposed by the emitted electron beam.

Thus, a large number of stimulable phosphor layer regions 27 formed on the support 26 of the stimulable phosphor sheet 25 are
Radiation data of radiolabeled substances are recorded.

FIG. 8 shows radiation data of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer regions 27 formed on the stimulable phosphor sheet 25 and a large number of biochemical analysis unit 1 data. FIG. 9 is a schematic perspective view of a scanner that reads fluorescence data recorded in the absorptive region 4 to generate biochemical analysis data, and FIG. 9 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner according to this embodiment is a unit for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer areas 27 formed on the stimulable phosphor sheet 25. It is configured to be able to read fluorescence data such as a fluorescent dye recorded in a large number of absorptive regions 4 of one, and a laser beam 34 having a wavelength of 640 nm.
And a second laser excitation light source 32 for emitting a laser beam 34 having a wavelength of 532 nm.
And a third laser excitation light source 33 that emits laser light 34 having a wavelength of 473 nm.

In the present embodiment, the first laser excitation light source 31 is composed of a semiconductor laser light source, and the second laser excitation light source 32 and the third laser excitation light source 33.
Is composed of a second harmonic generation element.

The laser light 34 generated by the first laser excitation light source 31 is collimated by the collimator lens 35 and then reflected by the mirror 46. First
In the optical path of the laser light 34 emitted from the laser excitation light source 31 and reflected by the mirror 46, the first dichroic mirror 37 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer dichroic mirror 37 A second dichroic mirror 48, which transmits light having a wavelength of 473 nm and reflects light having a wavelength of 473 nm, is provided.
Laser light 34 generated by the laser excitation light source 31 of
Passes through the first dichroic mirror 37 and the second dichroic mirror 48 and enters the mirror 39.

On the other hand, the laser light 34 generated from the second laser excitation light source 32 is passed by the collimator lens 40.
After being made into parallel light, it is reflected by the first dichroic mirror 37, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 48 and enters the mirror 39.

The laser light 34 generated from the third laser excitation light source 33 is collimated by the collimator lens 41, and then the second dichroic mirror 4 is irradiated.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 39.

The laser beam 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
A perforated mirror 44 formed by a concave mirror having a hole 43 formed in the center is arranged in the optical path of No. 4, and the laser light 34 reflected by the mirror 42 passes through the hole 43 of the perforated mirror 44. It passes through and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

The optical head 45 is provided with a mirror 46 and an aspherical lens 47. The laser light 34 incident on the optical head 45 is reflected by the mirror 46, and the aspherical lens 47 causes the glass plate of the stage 50 to be reflected. It is incident on the stimulable phosphor sheet 25 placed on 51 or the biochemical analysis unit 1.

A laser beam 34 is applied to the stimulable phosphor sheet 25.
Is excited, a large number of stimulable phosphor layer regions 27 formed on the support 26 of the stimulable phosphor sheet 25 are excited,
Bright luminescence 55 is emitted, and biochemical analysis unit 1
When the laser light 34 is incident on, the fluorescent substance such as the fluorescent dye contained in the many absorptive regions 4 is excited, and the fluorescence 55 is emitted.

The photostimulable light 55 emitted from the many stimulable phosphor layer regions 27 of the stimulable phosphor sheet 25 or the fluorescence 55 emitted from the many absorptive regions 4 of the biochemical analysis unit 1 are: An aspherical lens 47 provided in the optical head 45 collects the light on a mirror 46, reflects the light on the same side as the optical path of the laser light 34 by the mirror 46, collimates the light, and makes it enter a concave mirror 48.

The photostimulable light 55 or fluorescent light 55 that has entered the concave mirror 48 is reflected by the concave mirror 48,
The light enters the perforated mirror 44.

The photostimulable light 55 or the fluorescent light 55 which has entered the perforated mirror 44 is reflected downward by the perforated mirror 44 formed by a concave mirror and enters the filter unit 58, as shown in FIG. Then, the light of a predetermined wavelength is cut off, enters the photomultiplier 60, and is photoelectrically detected.

As shown in FIG. 9, the filter unit 58 includes four filter members 61a, 61b, 61c,
61d, the filter unit 58 is configured to be movable in the left-right direction in FIG. 8 by a motor (not shown).

FIG. 10 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 10, the filter member 6
1a includes a filter 62a, and the filter 62a includes a first
Is a filter member used when exciting the fluorescent substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 by using the laser excitation light source 31 and reading the fluorescence 55, Cut the light of 640 nm wavelength,
It has a property of transmitting light having a wavelength longer than 640 nm.

FIG. 11 is a sectional view taken along the line BB of FIG.

As shown in FIG. 11, the filter member 6
1b includes a filter 62b, and the filter 62b includes a second
Is a filter member used when exciting the fluorescent substance contained in a large number of absorptive regions 4 formed in the biochemical analysis unit 1 by using the laser excitation light source 32 and reading the fluorescence 55, Cuts light with a wavelength of 532 nm,
It has a property of transmitting light having a wavelength longer than 532 nm.

FIG. 12 is a sectional view taken along the line CC of FIG.

As shown in FIG. 12, the filter member 6
1c includes a filter 62c, and the filter 62c includes a third filter 62c.
Is a filter member used when the fluorescent substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 is excited by using the laser excitation light source 33 of FIG. , 473 nm is cut off, and light with a wavelength longer than 473 nm is transmitted.

FIG. 13 is a sectional view taken along the line DD of FIG.

As shown in FIG. 13, the filter member 6
1d includes a filter 62d, and the filter 62d includes a first
Of the stimulable phosphor sheet 2 using the laser excitation light source 31 of
The stimulable light 55 emitted from the stimulable phosphor layer region 27 by exciting the large number of stimulable phosphor layer regions 27 formed in FIG.
Is a filter used when reading, and has a property of transmitting only the light in the wavelength range of the stimulable light 55 emitted from the stimulable phosphor layer area 27 and cutting the light of the wavelength of 640 nm. There is.

Therefore, the filter members 61a, 61b, 61c, 61d, depending on the laser excitation light source to be used.
Is selectively placed in front of the photomultiplier 60, the photomultiplier 60 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processing device 64.

Although not shown in FIG. 8, the optical head 45 is configured to be movable in the main scanning direction indicated by arrow X and the sub scanning direction indicated by arrow Y in FIG. The entire surface of the biochemical analysis unit 1 is configured to be scanned by the laser light 34.

FIG. 14 is a schematic plan view of the scanning mechanism of the optical head. In FIG. 14, for simplification, the optical system except the optical head 45, the laser light 34, and the fluorescent light 5 are shown.
The optical path of 5 or stimulated emission 55 is omitted.

As shown in FIG. 14, the optical head 45
The scanning mechanism that scans the substrate 70 includes a substrate 70, and a sub-scanning pulse motor 71 and a pair of rails 72 and 62 are provided on the substrate 70.
14 are fixed, and the substrate 7 which is movable on the substrate 70 in the sub-scanning direction indicated by the arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 73, and a threaded rod 74 rotated by the sub-scanning pulse motor 71 is formed in this hole. Engaged.

A main scanning stepping motor 75 is provided on the movable substrate 73. The main scanning stepping motor 75 connects the endless belt 76 to the adjacent through holes 3 formed in the biochemical analysis unit 1, that is, the adjacent through holes 3. The stimulable phosphor layer 25 is formed so that it can be driven intermittently at a pitch equal to the distance between adjacent stimulable phosphor layer regions 27 formed on the stimulable phosphor sheet 25. The optical head 45 is fixed to the endless belt 76, and the main scanning stepping motor 7
5, when the endless belt 76 is driven, the endless belt 76 is moved in the main scanning direction indicated by an arrow X in FIG. In FIG. 14, 67 is a linear encoder for detecting the position of the optical head 45 in the main scanning direction, and 78 is a slit of the linear encoder 77.

Therefore, the main scanning stepping motor 7
5, the endless belt 76 is intermittently driven in the main scanning direction, and when scanning of one line is completed, the substrate 73 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 71. The optical head 45 is
In FIG. 14, all the stimulable phosphor layer areas 27 formed on the stimulable phosphor sheet 25 by the laser light 34 are moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y. Alternatively, all the absorptive regions 4 of the biochemical analysis unit 1 are scanned.

FIG. 15 is a block diagram showing the control system, input system, drive system and detection system of the scanner according to the preferred embodiment of the present invention.

As shown in FIG. 15, the control system of the scanner is a control unit 8 for controlling the entire scanner.
0, a data processing device 64, and a memory 55, and an input system of the scanner includes a keyboard 81 which is operated by a user and can input various instruction signals.

As shown in FIG. 15, the scanner drive system intermittently moves the optical head 45 in the main scanning direction and the main scanning stepping motor 75, and intermittently moves the optical head 45 in the sub scanning direction. Sub-scanning pulse motor 7
1 and 4 filter members 61a, 61b, 61c, 6
A filter unit motor 82 for moving the filter unit 58 having 1d is provided.

The control unit 80 includes a first laser pumping light source 31, a second laser pumping light source 32 or a third laser pumping light source 32.
In addition to selectively outputting the drive signal to the laser excitation light source 33, the drive signal can be output to the filter unit motor 82.

Further, as shown in FIG. 15, the detection system of the scanner is provided with a photomultiplier 60 and a linear encoder 77 for detecting the position of the optical head 45 in the main scanning direction.

In the present embodiment, the control unit 80 controls the first laser pumping light source 31, the second laser pumping light source 32 or the third laser pumping light source 32 according to the position detection signal of the optical head 45 input from the linear encoder 77. The laser excitation light source 33 is configured to be on / off controllable.

The scanner having the above-described structure is provided with a large number of photostimulable substances by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 as follows. The phosphor layer region 27 is exposed to read the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 25 to generate biochemical analysis data.

First, the stimulable phosphor sheet 25 is placed on the glass plate 51 of the stage 50.

Then, the keyboard 8 is set by the user.
An instruction signal for scanning a large number of stimulable phosphor layer regions 27 formed on the stimulable phosphor sheet 25 with the laser beam 34 is input to the first column.

The instruction signal input to the keyboard 81 is
Input to the control unit 80, the control unit 80 outputs a drive signal to the filter unit motor 82 in accordance with the instruction signal, and the filter unit 5
Photostimulable light 55 emitted from the stimulable phosphor by moving 8
The filter member 61d provided with the filter 62d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is located in the optical path of the stimulated light 55.

Further, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to move the optical head 45 in the main scanning direction, and based on the position detection signal of the optical head 45 input from the linear encoder, Of the many stimulable phosphor layer regions 27 formed on the stimulable phosphor sheet 25, the first stimulable phosphor layer region 2
7, at a position where the laser beam 34 can be irradiated, the optical head 4
When it is confirmed that 5 has been reached, a stop signal is output to the main scanning stepping motor 75 and a drive signal is output to the first laser excitation light source 31 to activate the first laser excitation light source 31, Laser light 34 having a wavelength of 640 nm
To emit.

The laser light 34 emitted from the first laser excitation light source 31 is made into parallel light by the collimator lens 35, and then enters the mirror 46 and is reflected.

Laser light 3 reflected by the mirror 46
The light beam 4 passes through the first dichroic mirror 37 and the second dichroic mirror 48 and enters the mirror 39.

The laser light 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
4 passes through the hole 43 of the perforated mirror 44 and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

Laser light 34 incident on the optical head 45
Is reflected by the mirror 46, and by the aspherical lens 47, the first stimulable phosphor layer area 27 of the stimulable phosphor sheet 25 placed on the glass plate 51 of the stage 50.
Is focused on.

As a result, the stimulable phosphor contained in the first stimulable phosphor layer region 27 formed on the substrate 26 of the stimulable phosphor sheet 25 is excited by the laser beam 34 to generate the first stimulable phosphor. Photostimulation light 55 is emitted from the photostimulable phosphor layer region 27 of FIG.

The stimulable light 55 emitted from the first stimulable phosphor region 15 is condensed by the aspherical lens 47 provided in the optical head 45, and is the same as the optical path of the laser light 34 by the mirror 46. Is reflected to the side and made into parallel light,
It is incident on the concave mirror 48.

The photostimulable light 55 incident on the concave mirror 48 is
The perforated mirror 44 is reflected by the concave mirror 48.
Incident on.

[0229] The photostimulable light 55 incident on the perforated mirror 44.
Is reflected downward by the perforated mirror 44 formed by the concave mirror and enters the filter 62d of the filter unit 58, as shown in FIG.

The filter 62d transmits only the light in the wavelength range of the stimulable light 55 emitted from the stimulable phosphor, and emits 640n.
Since it has a property of cutting off light having a wavelength of m, light having a wavelength of 640 nm, which is excitation light, is cut, and light in the wavelength range of the stimulable light 55 emitted from the stimulable phosphor layer region 27. Only the light passes through the filter 62d and is photoelectrically detected by the photomultiplier 60.

The analog signal photoelectrically detected and generated by the photomultiplier 60 is output to the A / D converter 63, converted into a digital signal, and output to the data processing device 64.

After the first laser excitation light source 31 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 80 outputs a drive stop signal to the first laser excitation light source 31, The driving of the first laser excitation light source 31 is stopped, and a driving signal is output to the main scanning stepping motor 75 to cause the optical head 45 to move to the adjacent stimulable phosphors formed on the stimulable phosphor sheet 25. It is moved by a pitch equal to the distance between the layer regions 27.

Based on the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Are moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 27, and the first laser excitation light source 31
The stimulable phosphor sheet 2 emits the laser light 34 emitted from the
When it is confirmed that the second stimulable phosphor layer area 27 formed in No. 5 has moved to a position where irradiation is possible, the control unit 80 outputs a drive signal to the first laser excitation light source 31. , Turn on the first laser excitation light source 31,
The laser light 34 excites the stimulable phosphor contained in the second stimulable phosphor layer region 27 formed on the stimulable phosphor sheet 25.

Similarly, the laser beam 34 is applied to the second photostimulable phosphor layer region 27 formed on the substrate 26 of the stimulable phosphor sheet 25 for a predetermined time, and the second photostimulable phosphor layer region 27 is irradiated. The stimulable phosphor contained in the stimulable phosphor layer region 27 is excited, and the stimulable light 55 emitted from the second stimulable phosphor layer region 27 is converted by the photomultiplier 60 into a photomultiplier 60.
When photoelectrically detected and analog data is generated,
The control unit 80 includes the first laser excitation light source 3
1 to turn off the first laser excitation light source 31 and to output the main scanning stepping motor 75.
Then, a drive signal is output to move the optical head 45 by one pitch equal to the distance between adjacent photostimulable phosphor layer regions 27.

Thus, the first laser excitation light source 31 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 45, and based on the position detection signal of the optical head 45 input from the linear encoder 77, Optical head 45
However, when it is confirmed that the scanning by the laser beam 34 on the stimulable phosphor layer area 27 on the first line has been completed by being moved by one line in the main scanning direction, the control unit 80 causes the main scanning A drive signal is output to the stepping motor 75 to return the optical head 45 to the original position, and a drive signal is output to the sub-scanning pulse motor 71.
The movable substrate 73 is moved by one line in the sub-scanning direction.

Based on the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is returned to its original position, and the movable substrate 73 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 80 sequentially moves from the first laser excitation light source 31 to the stimulable phosphor layer area 27 on the first line. The stimulable phosphor layer region 27 of the second line is processed in the same manner as the irradiation of the emitted laser beam 34.
Are sequentially irradiated with laser light 34 emitted from the first laser excitation light source 31 to excite the stimulable phosphor contained in the stimulable phosphor layer region 27, thereby stimulating the stimulable phosphor layer. Area 2
The photostimulated light 55 emitted from 7 is sequentially photoelectrically detected by the photomultiplier 60.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processing device 64.

In this way, all the many stimulable phosphor layer regions 27 formed on the substrate 26 of the stimulable phosphor sheet 25 are scanned by the laser light 34 emitted from the first laser excitation light source 31, and many Stimulable phosphor layer region 27 of
The stimulable phosphor contained in is excited, and the emitted stimulable light 55 is photoelectrically detected by the photomultiplier 60, and the generated analog data is converted by the A / D converter 63. When converted into digital data and sent to the data processing device 64, the control unit 8
From 0, the drive stop signal is output to the first laser excitation light source 31, and the drive of the first laser excitation light source 31 is stopped.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 4 formed in the biochemical analysis unit 1 is read to generate the biochemical analysis digital data, first, the operator The biochemical analysis unit 1 is set on the glass plate 51 of the stage 50.

Next, the operator specifies the type of the fluorescent substance as the labeling substance and inputs the instruction signal to the effect that the fluorescent data should be read, to the keyboard 81.

The instruction signal input to the keyboard 81 is
When the control unit 80 receives the instruction signal and receives the instruction signal, the control unit 80 determines the laser excitation light source to be used according to the table stored in the memory (not shown) and the filter 62.
It is determined which of a, 62b, and 62c is to be located in the optical path of the fluorescent light 55.

For example, Rhodamine (registered trademark), which can be most efficiently excited by a laser having a wavelength of 532 nm, as a fluorescent substance for labeling a substance of biological origin
Is used and is input to the keyboard 81, the control unit 80 selects the second laser excitation light source 32, selects the filter 62b, and outputs a drive signal to the filter unit motor 82. Then, the filter unit 58 is moved to cut off the light having the wavelength of 532 nm, and the filter member 61b having the property of transmitting the light having the wavelength longer than 532 nm is released from the biochemical analysis unit 1. It is located in the optical path of the fluorescent light 55 to be formed.

Further, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to move the optical head 45 in the main scanning direction, and based on the position detection signal of the optical head 45 inputted from the linear encoder, When it is confirmed that the optical head 45 has reached the position where the laser beam 34 can be irradiated to the first absorptive region 4 among the many absorptive regions 4 formed in the biochemical analysis unit 1. , A stop signal is output to the main scanning stepping motor 75, and a drive signal is output to the second laser excitation light source 32 to activate the second laser excitation light source 32.
A laser beam 34 having a wavelength of 532 nm is emitted.

The laser light 34 emitted from the second laser excitation light source 32 is made into parallel light by the collimator lens 40, and then enters the first dichroic mirror 37 and is reflected.

The laser beam 34 reflected by the first dichroic mirror 37 passes through the second dichroic mirror 48 and enters the mirror 39.

The laser beam 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
4 passes through the hole 43 of the perforated mirror 44 and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

Laser light 34 incident on the optical head 45
Is reflected by the mirror 46, and is focused by the aspherical lens 47 on the biochemical analysis unit 1 mounted on the glass plate 51 of the stage 50.

As a result, the laser beam 34 excites a fluorescent substance such as a fluorescent dye contained in the first adsorptive region 4 of the biochemical analysis unit 1, for example, rhodamine to emit fluorescence.

Here, in the biochemical analysis unit 1 according to the present embodiment, a large number of absorptive regions 4 are formed on the substrate 2 which is made of aluminum and has a property of attenuating light so as to be spaced apart from each other. Therefore, the fluorescent substance contained in the absorptive region 4 is excited, and the fluorescent light 55 emitted from the fluorescent substance is excited and emitted from the fluorescent substance contained in the adjacent absorptive region 4. Mixing with the fluorescent light 55 can be reliably prevented.

The fluorescent light 55 emitted from the rhodamine is condensed by the aspherical lens 47 provided in the optical head 45, reflected by the mirror 46 to the same side as the optical path of the laser light 34, and made into parallel light. , Enters the concave mirror 48.

The fluorescent light 55 that has entered the concave mirror 48 is reflected by the concave mirror 48 and enters the perforated mirror 44.

Fluorescent light 55 that has entered the perforated mirror 44 is
The perforated mirror 44 formed by the concave mirror reflects the light downward as shown in FIG. 8 and makes it incident on the filter 62b of the filter unit 58.

Since the filter 62b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm, which is excitation light, is cut and emitted from rhodamine. Fluorescence 55
Only the light in the wavelength range of (4) passes through the filter 62b and is photoelectrically detected by the photomultiplier 60.

The analog signal photoelectrically detected by the photomultiplier 60 and generated is output to the A / D converter 63, converted into a digital signal, and output to the data processing device 64.

When a predetermined time, for example, several microseconds has elapsed after the second laser excitation light source 32 was turned on, the control unit 80 outputs a drive stop signal to the second laser excitation light source 32, The drive of the second laser excitation light source 32 is stopped, and a drive signal is output to the main scanning stepping motor 75 to cause the optical head 45 to move to the adsorbing regions 4 adjacent to each other formed in the biochemical analysis unit 1.
Move by a pitch equal to the distance between.

On the basis of the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is moved by one pitch equal to the distance between the adjacent absorptive regions 4 formed in the biochemical analysis unit 1,
Laser light 34 emitted from the second laser excitation light source 32
Is confirmed to have moved to a position where the second absorptive region 4 formed in the biochemical analysis unit 1 can be irradiated, the control unit 80 sends a drive signal to the second laser excitation light source 32. Output the second laser excitation light source 3
2 is turned on, and the laser beam 34 excites the fluorescent substance, for example, rhodamine, contained in the second absorptive region 4 formed in the biochemical analysis unit 1.

Similarly, the laser light 34 irradiates the second absorptive region 4 formed in the biochemical analysis unit 1 for a predetermined time, and the fluorescence emitted from the second absorptive region 4 is emitted. When 55 is photoelectrically detected by the photomultiplier 60 and analog data is generated, the control unit 80 outputs an off signal to the second laser pumping light source 32 to output the second laser pumping light source. Three
2 is turned off and the main scanning stepping motor 7
5, a drive signal is output to move the optical head 45 by one pitch equal to the distance between the adsorbing regions 4 adjacent to each other formed in the biochemical analysis unit 1.

In this way, the first laser excitation light source 31 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 45, and based on the position detection signal of the optical head 45 input from the linear encoder 77, Optical head 45
Is moved by one line in the main scanning direction, and all the absorptive regions 4 of the first line of the biochemical analysis unit 1 are moved.
When it is confirmed that the laser beam 34 is scanned by the laser beam 34, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to return the optical head 45 to the original position, and at the same time, the sub scanning pulse motor. 71
A driving signal is output to the movable substrate 73 to move the movable substrate 73 by one line in the sub-scanning direction.

Based on the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is returned to its original position, and the movable substrate 73 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 80 causes the absorptive region 4 of the first line formed in the biochemical analysis unit 1 to be formed.
Then, in the same manner as irradiating the laser beam 34 emitted from the second laser excitation light source 32 in sequence, the absorptive region 4 of the second line formed in the biochemical analysis unit 1
The fluorescence 55 emitted from the adsorptive region 4 by exciting the rhodamine contained in the photomultiplier 6
0 causes photoelectric detection.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processing device 64.

In this way, all the absorptive regions 4 formed in the biochemical analysis unit 1 are scanned by the laser light 34 emitted from the second laser excitation light source 32 and formed in the biochemical analysis unit 1. A large number of absorptive areas 4
Rhodamine contained in is excited and the emitted fluorescence 55 is photoelectrically detected by the photomultiplier 60, and the generated analog data is converted into digital data by the A / D converter 63. , The control unit 80 when sent to the data processing device 64.
Then, the drive stop signal is output to the second laser excitation light source 32, and the drive of the second laser excitation light source 32 is stopped.

According to this embodiment, the hybridization bag 7 is provided with a pair of holding parts 9 for holding the hybridization bag 7 at both edges thereof, and the pair of holding parts 9 each have a holding part 9. Since the perforations 10 are formed along the longitudinal direction, by providing the endless belts 12 and 12 with the claws that engage with the perforations 10 formed on the hybridization bag 7, it is possible to increase the height of a thin sheet. The hybridization bag 7 can be easily transported as desired, and therefore, the hybridization can be easily automated, so that great labor saving can be realized.

Further, according to this embodiment, the first solution injecting portion 11a and the second solution injecting portion 11b, which are cylindrical, are formed on one side of the holding portion 9 so as to cross the holding portion 9, respectively. The pretreatment liquid, the hybridization solution, or the probe solution can be injected into the hybridization bag 7 by inserting the pretreatment liquid pin 16, the hybridization solution injection pin 17, or the probe solution injection pin 18. The hybridization bag 7 can be attached to the endless belt 12,
By reciprocating between the pair of squeeze rollers 15 by 12, the pretreatment solution, the hybridization solution or the solution obtained by adding the probe solution to the hybridization solution contained in the hybridization bag 7 is agitated, It is possible to bring the biochemical analysis unit 1 into uniform contact with the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7.
Pre-treatment, pre-hybridization and hybridization can be automatically carried out simply by sandwiching them between 2 and 12 and setting them in the hybridization device.

Further, in the present embodiment, the hybridization apparatus is provided with the cutter 13 and the heater 14, and since the hybridization bag 7 is formed of the thermoplastic sheet, the second bag is formed after the pretreatment is completed. The portion of the hybridization bag 7 in which the solution injection portion 11b is formed is cut to form an opening, and the endless belts 12 and 12 are driven to move the hybridization bag 7 between the pair of squeeze rollers 15. The pretreatment liquid is discharged from the hybridization bag 7 by passing it through, the hybridization bag 7 is sealed, the opening is closed, and then the hybridization solution is injected from the first solution injection part 11a. , Perform prehybridization, and further From the solution injection unit 11a, by injecting probe solution, perform hybridization, after completion of the hybridization, the first solution injection section 1
The portion of the hybridization bag 7 on which 1a is formed is cut to form an opening, and the endless belt 1
2 and 12 drive the hybridization bag 7
By passing between the pair of squeeze rollers 15, the solution in which the probe solution is added to the hybridization solution can be discharged from the hybridization bag 7 by the pair of squeeze rollers 15. In addition, it becomes possible to carry out hybridization.

Further, according to this embodiment, the first solution injecting portion 11a and the second solution injecting portion 11b are each formed of elastic rubber compressed in the radial direction,
While the pin can be inserted, when the pin is pulled out, the hole formed when the pin is inserted is formed so as to be closed. The hybridization solution and the probe solution can be injected into the hybridization bag 7, and after the injection of the pretreatment liquid, the hybridization solution and the probe solution, the pair of squeeze rollers 15 can be used without sealing the hybridization bag 7. By this, it becomes possible to stir the solution in which the pretreatment liquid, the hybridization solution and the probe solution are added to the hybridization solution, and therefore, the hybridization operation can be simplified.

FIG. 16 is a schematic perspective view of a hybridization bag 7 according to another preferred embodiment of the present invention.

As shown in FIG. 16, in the hybridization bag 7 according to this embodiment, the first solution injection part 91a, the second solution injection part 91b and the third solution injection part 91c are the same as the hybridization bag. The hybridization bag 7 shown in FIG. 3 except that it is formed on one of the holding parts 9 formed on both edges of the hybridization bag 7.
It has the same configuration as.

In this embodiment, the first solution injection section 9
1a, the second solution injection part 91b and the third solution injection part 91c are respectively formed of soft plastic, and the pretreatment liquid pin 16, the hybridization solution injection pin 17 and the probe solution injection pin 18 are inserted therethrough. Is configured to be able to.

Also in this embodiment, similarly to the above-described embodiment, the user holds the pair of holding portions 9 between the pair of upper and lower endless drums 12, 12 to form the pair of holding portions 9. The hybridization bag 7 is set in the hybridization device so that the perforations 10 are engaged with the claws (not shown) of the pair of upper and lower endless drums 12, 12 and a start signal is input to the keyboard 24. .

The start signal is the control unit 2
When the control unit 25 receives the start signal, the control unit 25 outputs a drive signal to the endless belt motor 26 to move the endless belts 12 and 12 to the position shown in FIG.
In, the drive is performed in the positive direction indicated by arrow A.

As a result, the perforations 1 formed on the pair of holding portions 9 of the hybridization bag 7
Since the claws (not shown) of the endless drums 12 and 12 are engaged with the hybridization bag 7
Are moved in the positive direction indicated by arrow A in FIG.

The hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the third solution injection portion 91c formed in the holding portion 9 of the hybridization bag 7 reaches the position facing the pin head 19. Then,
A drive stop signal is output from the control unit 20 to the endless belt motor 21, and the endless belts 12, 12 are stopped.

Next, the control unit 20 outputs a pretreatment liquid injection pin drive signal to the pin head motor 22 so that the pretreatment liquid injection pin 16 held by the pin head 19 is held in the holding portion 9 of the hybridization bag 7. The pin head 19 is attached to the endless belt 1 so as to be located at a position facing the formed third solution injection part 91c.
2 is moved in a direction parallel to the driving direction of the endless belt 12, and then the pretreatment liquid injection pin 16 is formed in the holding solution 9 of the hybridization bag 7 in a direction orthogonal to the driving direction of the endless belt 12. The pin head 19 is moved until it is inserted into the injection portion 91c.

In this way, when the inside of the hybridization bag 7 is communicated with the pretreatment liquid tank (not shown) through the pretreatment liquid pin 16, the control unit 20 sends a drive stop signal to the pin head motor 22. Then, the pin head 19 is stopped, and a drive signal is further output to the pretreatment liquid pump 23a so that the pretreatment liquid contained in the pretreatment liquid tank is driven to a high level via the pretreatment liquid injection pin 16. It is injected into the hybridization bag 7.

When a predetermined amount of pretreatment liquid has been injected into the hybridization bag 7 after a predetermined time has elapsed, the control unit 20 causes the pretreatment liquid pump 2 to
A drive stop signal is output to 3a to stop the supply of the pretreatment liquid, and then a retract signal is output to the pin head motor 22 to return the pin head 19 to the original position.

At the same time, the control unit 20 outputs a reverse driving signal to the endless belt motor 21 to move the endless belts 12, 12 to the arrow B in FIG.
Drive in the direction indicated by.

[0279] When the pretreatment liquid injection pin 16 is pulled out,
Although a hole is formed in the third solution injecting section 91c, the hybridization bag 7 has a pair of holding sections 9 sandwiched and conveyed by the endless belts 12 and 12, and a pair of squeeze rollers 15 are provided. Therefore, the pretreatment liquid contained in the hybridization bag 7 does not leak from the hole formed in the third solution injection portion 91c.

The endless belts 12 and 12 are driven in the direction shown by the arrow B, and the perforations 10 adjacent to the third solution injecting portion 91c of the hybridization bag 7 pass through the heater 14 to inject the third solution. When the portion 91c reaches just before the heater 14, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 and turn on the heater 14.

As a result, the third solution injecting section 91c and the perforation 1 adjacent to the third solution injecting section 91c.
Between 0, the hybridization bag 7 is heat sealed.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21,
The endless belts 12, 12 are driven in the direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 16, and the seal portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the pair of squeeze rollers 15 stir the pretreatment liquid injected into the hybridization bag 7, and the pretreatment liquid is stored in the hybridization bag 7. Pretreatment is performed by uniformly contacting the absorptive region 4 of the chemical analysis unit 1.

When the heat-sealed portion of the hybridization bag 7 reaches just before the pair of squeeze rollers 15, the control unit 20
A drive stop signal is output to the endless belt motor 21 to stop the drive of the endless belts 12 and 12, and then a drive signal is output to the endless belt motor 21 to move the endless belt motors 12 and 12 in FIG. It is driven in the positive direction indicated by arrow A.

As a result, the pair of squeeze rollers 15 stir the pretreatment liquid injected into the hybridization bag 7, and the pretreatment liquid is stored in the hybridization bag 7. The uniform contact with the absorptive region 4 of No. 3 and the pretreatment is accelerated.

In this way, the pretreatment liquid contained between the heat-sealed portion of the hybridization bag 7 and the seal portion 8a is agitated by the pair of squeeze rollers 15, and the pretreatment liquid is transferred to the hybridization bag 7 The adsorbent region 4 of the biochemical analysis unit 1 housed therein is uniformly contacted, and the pretreatment proceeds.

When the above operation is repeated a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. A drive signal is output to the belt motor 21 so that the heat-sealed portion of the hybridization bag 7 passes through the cutter 13 until the endless belt 1
2 and 12 are driven.

When the heat-sealed portion of the hybridization bag 7 passes through the cutter 13, the control unit 20 outputs a drive stop signal to the endless belt motor 21 and the endless belts 12, 12 are fed.
Drive is stopped, and then a drive signal is output to the cutter 13 to cut the hybridization bag 7 between the heat-sealed portion and the perforations 10 adjacent thereto, and the heat-sealed portion is Three
The opening (not shown) is formed in the hybridization bag 7 together with the solution injection part 91c.

Then, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to move the endless belts 12, 12 to the arrow B in FIG.
Drive in the reverse direction indicated by.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 16, and the seal portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, however, the pretreatment liquid injected into the inside of the hybridization bag 7 is squeezed out by the pair of squeeze rollers 15 through the opening (not shown).

The squeezed pretreatment liquid is recovered in a pretreatment liquid recovery tank (not shown) arranged below the endless belts 12, 12.

Next, the control unit 20 outputs a drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the forward direction indicated by arrow A in FIG.

As a result, the hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the cutter 1
The opening formed by being cut by 3 is the heater 1
When it reaches the position facing 4, the control unit 2
0 outputs a driving stop signal to the endless belt motor 21, stops driving the endless belts 12 and 12, turns on the heater 14, and heat seals the opening formed in the hybridization bag 7.

Next, the control unit 20 outputs a drive signal to the endless belt motor 21 to drive the endless belts 12, 12 in the forward direction indicated by the arrow A in FIG. 16 to hold the hybridization bag 7. Second solution injection part 91b formed in part 9
However, when it reaches a position facing the pin head 19, the endless belt motor 21
The drive stop signal is output to the endless belt 1
2, 12 are stopped.

Next, the control unit 20 outputs a hybridization solution injection pin drive signal to the pin head motor 22, and the hybridization solution injection pin 17 held by the pin head 19 is formed in the holding portion 9 of the hybridization bag 7. The pin head 19 is moved in a direction parallel to the driving direction of the endless belt 12 so as to be positioned at a position opposite to the second solution injecting portion 91 b, and then in a direction orthogonal to the driving direction of the endless belt 12. , The pin head 19 is moved until the hybridization solution injection pin 17 is inserted into the second solution injection part 91b formed in the holding part 9 of the hybridization bag 7.

Thus, the hybridization bag 7 is inserted through the hybridization solution injection pin 17.
When the inside of the container is communicated with a hybridization solution tank (not shown), the control unit 20 outputs a drive stop signal to the pin head motor 22 to stop the pin head 19, and further to the hybridization solution pump 23b. A drive signal is output to inject the hybridization solution contained in the hybridization solution tank into the hybridization bag 7 via the hybridization solution injection pin 17.

When a predetermined amount of hybridization solution has been injected into the hybridization bag 7 after a predetermined time has passed, the control unit 20
Outputs a drive stop signal to the hybridization solution pump 23b to stop the supply of the hybridization solution, and then outputs a retract signal to the pin head motor 22 to return the pin head 19 to the original position.

At the same time, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to move the endless belts 12, 12 to the arrow B in FIG.
Drive in the direction indicated by.

Hybridization Solution Injection Pin 17
Is pulled out, a hole is formed in the second solution injecting section 91b, but in the hybridization bag 7, the pair of holding sections 9 is formed by the endless belts 12, 12.
Is carried by the pair of squeeze rollers 15 and not carried by the hybridization bag 7.
The hybridization solution accommodated in the first solution does not leak from the hole formed in the second solution injection part 91b.

The endless belts 12 and 12 are driven in the direction indicated by the arrow B, and the perforations 10 adjacent to the second solution injecting portion 91b of the hybridization bag 7 pass through the heater 14 to inject the second solution. When the portion 91b reaches just before the heater 14, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 and turn on the heater 14.

As a result, the second solution injection section 91b and the perforation 1 adjacent to the second solution injection section 91b.
Between 0, the hybridization bag 7 is heat sealed.

Then, the control unit 20 outputs a reverse drive signal to the endless belt motor 21,
The endless belts 12, 12 are driven in the direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction indicated by the arrow B in FIG. 16, and the sealing portion 8a of the hybridization bag 7 is moved.
When passing through the pair of squeeze rollers 15, the pair of squeeze rollers 15 stir the hybridization solution injected into the hybridization bag 7, and the hybridization solution is stored in the hybridization bag 7. Pre-hybridization is performed by making uniform contact with the adsorptive region 4 of the chemical analysis unit 1.

When the heat-sealed portion of the hybridization bag 7 reaches just before the pair of squeeze rollers 15, the control unit 20
A drive stop signal is output to the endless belt motor 21 to stop the drive of the endless belts 12 and 12, and then a drive signal is output to the endless belt motor 21 to move the endless belts 12 and 12 in FIG.
It is driven in the positive direction indicated by arrow A.

As a result, the pair of squeeze rollers 15 agitate the hybridization solution injected into the hybridization bag 7, and the hybridization solution is stored in the hybridization bag 7. The uniform contact with the absorptive region 4 of No. 1 promotes prehybridization.

In this way, the hybridization solution contained between the heat-sealed portion of the hybridization bag 7 and the seal portion 8a is agitated by the pair of squeeze rollers 15, and the hybridization solution is transferred to the hybridization bag 7. Pre-hybridization proceeds by uniformly contacting the absorptive region 4 of the biochemical analysis unit 1 housed therein.

When the above operation is repeated a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. A drive signal is output to the belt motor 21 to drive the endless belts 12, 12.

As a result, the hybridization bag 7 is moved in the forward direction indicated by the arrow A, and the first solution injection part 91a formed in the holding part 9 of the hybridization bag 7 faces the pin head 19. When the position is reached, a drive stop signal is output from the control unit 20 to the endless belt motor 21, and the endless belts 12, 12 are stopped.

Then, the control unit 20 sends the probe solution injection pin drive signal to the pin head motor 22.
To the probe solution injection pin 18 held by the pin head 19 so as to be positioned at a position facing the first solution injection part 91a formed in the holding part 9 of the hybridization bag 7. The endless belt 12 is moved in a direction parallel to the driving direction, and then
The probe solution injection pin 17 is formed in the holding portion 9 of the hybridization bag 7 in the direction orthogonal to the driving direction of the endless belt 12, and the first solution injection portion 91a.
The pin head 19 is moved until it is inserted inside.

Thus, when the inside of the hybridization bag 7 communicates with the probe solution solution tank (not shown) via the probe solution injection pin 18, the control unit 20 sends a drive stop signal to the pin head motor 22. Output, stop the pin head 19, and further output a drive signal to the probe solution pump 23c,
The probe solution stored in the probe solution tank,
The probe solution is injected into the hybridization bag 7 via the probe solution injection pin 18, and the probe solution is added to the hybridization solution in the hybridization bag 7.

When a predetermined time has elapsed and a predetermined amount of the probe solution is injected into the hybridization bag 7 and added to the hybridization solution, the control unit 20 causes the probe solution pump 23c to operate.
After the drive stop signal is output to stop the supply of the probe solution, a retract signal is output to the pin head motor 22 to return the pin head 19 to the original position.

At the same time, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to move the endless belts 12, 12 to the arrow B in FIG.
Drive in the direction indicated by.

Hybridization solution injection pin 17
Is pulled out, a hole is formed in the first solution injecting section 91a, but in the hybridization bag 7, the pair of holding sections 9 is formed by the endless belts 12, 12.
Is carried by the pair of squeeze rollers 15 and not carried by the hybridization bag 7.
The hybridization solution and probe solution housed in the first solution do not leak out from the hole formed in the first solution injection part 91a.

The endless belts 12, 12 are driven in the direction shown by the arrow B, and the perforations 10 adjacent to the first solution injecting portion 91a of the hybridization bag 7 pass through the heater 14 to inject the first solution. When the portion 91a reaches just before the heater 14, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 and turn on the heater 14.

As a result, the first solution injecting section 91a and the perforation 1 adjacent to the first solution injecting section 91a.
Between 0, the hybridization bag 7 is heat sealed.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21,
The endless belts 12, 12 are driven in the direction indicated by arrow B in FIG.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 16, and the seal portion 8a of the hybridization bag 7 is moved.
However, when passing through the pair of squeeze rollers 15, the probe solution is added to the hybridization solution in the hybridization bag 7 by the pair of squeeze rollers 15 and the prepared solution is agitated to obtain the hybridization solution. The solution to which the probe solution is added uniformly contacts the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7, and hybridization is performed.

When the heat-sealed portion of the hybridization bag 7 reaches just before the pair of squeeze rollers 15, the control unit 20
A drive stop signal is output to the endless belt motor 21 to stop the drive of the endless belts 12 and 12, and then a drive signal is output to the endless belt motor 21 so that the endless belt motor 21 shown in FIG.
It is driven in the positive direction indicated by arrow A.

As a result, the solution in which the probe solution was added to the hybridization solution in the hybridization bag 7 was agitated by the pair of squeeze rollers 15, and the solution in which the probe solution was added was changed to the hybridization solution. The pretreatment is promoted by uniformly contacting the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7.

In this way, the solution obtained by adding the probe solution to the hybridization solution housed between the heat-sealed portion of the hybridization bag 7 and the sealing portion 8a is stirred by the pair of squeeze rollers 15, The solution obtained by adding the probe solution to the hybridization solution uniformly contacts the absorptive region 4 of the biochemical analysis unit 1 housed in the hybridization bag 7, and the hybridization proceeds.

When the above operation is repeated a predetermined number of times, the control unit 20 outputs a drive stop signal to the endless belt motor 21 to stop the drive of the endless belts 12, 12 once, and then the endless belts 12 and 12. A drive signal is output to the belt motor 21 so that the heat-sealed portion of the hybridization bag 7 passes through the cutter 13 until the endless belt 1
2 and 12 are driven.

When the heat-sealed portion of the hybridization bag 7 passes through the cutter 13, the control unit 20 outputs a drive stop signal to the endless belt motor 21 and the endless belts 12, 12 are fed.
Is stopped, and then a drive signal is output to the cutter 13 to cut the hybridization bag 7 between the heat-sealed portion and the perforations 10 adjacent thereto, and the heat-sealed portion is moved to the second position. Together with the solution injection part 91b and the first solution injection part 91a of
An opening (not shown) is formed.

Next, the control unit 20 outputs a reverse drive signal to the endless belt motor 21 to move the endless belts 12, 12 to the arrow B in FIG.
Drive in the reverse direction indicated by.

As a result, the hybridization bag 7 is moved in the opposite direction shown by the arrow B in FIG. 16, and the seal portion 8a of the hybridization bag 7 is moved.
However, when passing through the pair of squeeze rollers 15, the probe solution is added to the hybridization solution contained in the hybridization bag 7 by the pair of squeeze rollers 15 so that the solution has an opening (not shown). Is squeezed through.

The solution prepared by adding the probe solution to the squeezed hybridization solution is collected in the hybridization solution (not shown) arranged below the endless belts 12, 12.

When the solution in which the probe solution is added to the hybridization solution is discharged from the hybridization bag 7, the hybridization bag 7
Are collected from the hybridization apparatus, the biochemical analysis unit 1 is taken out from the hybridization bag 7, and washed in the container containing the cleaning solution.

In this way, the radiation data of the radioactive labeling substance as the labeling substance and the fluorescence data of the fluorescent substance such as the fluorescent dye are recorded in the many absorptive regions 4 of the biochemical analysis unit 1. The fluorescence data recorded in the absorptive region 4 are shown in FIGS.
The data is read by the scanner shown in Fig. 3 and biochemical analysis data is generated.

On the other hand, the radiation data of the radiolabeled substance is
In the same manner as in the above embodiment, the radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by the scanner shown in FIGS. 8 to 15, and biochemical analysis data is obtained. Is generated.

According to this embodiment, the first solution injection section 9
Since the 1a, the second solution injection section 91b and the third solution injection section 91c are made of soft plastic, the hybridization bag 7 can be manufactured easily and at low cost. . The present invention is
Without being limited to the above embodiment, various modifications are possible within the scope of the invention described in the claims,
It goes without saying that they are also included in the scope of the present invention.

For example, in the embodiment shown in FIG. 3, the hybridization bag 7 is provided with the first solution injecting portion 11a and the second solution injecting portion 11b. When the biochemical analysis unit 1 is used, one of the first solution injection part 11a and the second solution injection part 11b may be formed in the hybridization bag 7.

In the embodiment shown in FIG. 16, the hybridization bag 7 is provided with a first solution injection part 91a, a second solution injection part 91b and a third solution injection part 91c. However, when the biochemical analysis unit 1 that has been subjected to the pretreatment is separately used, the first solution injection part 91a, the second solution injection part 91b and the third solution injection part 91a are used.
Of the solution injecting parts 91c of 2 above, two solution injecting parts may be formed in the hybridization bag 7.

Further, in the above-described embodiment, the hybridization apparatus is provided with the pretreatment liquid injection pin 16, the hybridization solution injection pin 17 and the probe solution injection pin 18, but the pretreatment liquid pin 16 is omitted. You can also

Furthermore, in the above-described embodiment, the hybridization bag 7 is conveyed in both the forward and reverse directions by using the endless belts 12, 12 having the nails formed on the surface thereof. By engaging the perforations 10 formed on the edges,
Any driving means can be used as long as it can convey the hybridization bag 7, and a gear mechanism or the like can be used instead of the endless belts 12, 12.

In the above embodiment, the hybridization bag 7 is provided at a position near the seal portion 8a,
The cutter 13 is provided, and the heater 14 for sealing is provided at a more distant position.
4 may be arranged in reverse.

Furthermore, in the above embodiment, the pretreatment liquid injection pin 16, the hybridization solution injection pin 17 and the probe solution injection pin 18 are the pin head 1
9 and is configured to be moved by the pin head motor 22.
6. The hybridization solution injection pin 17 and the probe solution injection pin 18 can be configured to be moved by independent driving means.

Further, in the above-mentioned embodiment, the hybridization device is provided with a pair of squeeze rollers 15, but it is also possible to provide two or more pairs of squeeze rollers 15.

Furthermore, in the embodiment shown in FIG. 3, the first solution injection part 11a and the second solution injection part 1 are
The pins 1b are made of elastic rubber compressed in the radial direction, respectively, and the pins can be inserted, while the holes formed when the pins are inserted are closed when the pins are pulled out. So that the first solution injection part 1
It suffices that 1a and the second solution injecting portion 11b are formed, and it is not always necessary to form the first solution injecting portion 11a and the second solution injecting portion 11b by the elastic rubber compressed in the radial direction. .

Further, in the above-mentioned embodiment, the substrate 2 of the biochemical analysis unit 1 is provided with about 0.0
The substantially circular adsorptive regions 4 having a size of 1 square millimeter have a density of about 5000 pieces / square centimeter,
Although formed according to a regular pattern, the absorptive region 4 does not necessarily need to be formed in a substantially circular shape, and can be formed in any shape such as a rectangular shape.

In the above-mentioned embodiment, the substrate 2 of the biochemical analysis unit 1 is provided with about 0.01 of about 10,000.
The substantially circular adsorptive regions 4 having a size of square millimeter are formed according to a regular pattern with a density of about 5000 pieces / square centimeter, but the number and size of the adsorptive regions 4 are determined according to the purpose. Therefore, the number of adsorbent regions 4 having a size of 10 or more and less than 5 mm 2 is 10 or more.
It is formed on the substrate 2 with a density of at least one piece / square centimeter.

Further, in the above-mentioned embodiment, the substrate 2 of the biochemical analysis unit 1 has about 0.0
The substantially circular adsorptive regions 4 having a size of 1 square millimeter have a density of about 5000 pieces / square centimeter,
Although formed according to a regular pattern, it is not always necessary to form the absorptive region 4 or the spot-like region 174 according to a regular pattern.

In the above embodiment, the biochemical analysis unit 1 has nylon 6 filled inside the many through holes 3 formed in the substrate 2 made of aluminum.
Although it has a large number of absorptive regions 4 formed, it is not always necessary that the absorptive regions 4 of the biochemical analysis unit 1 be formed of nylon 6, and a membrane filter other than nylon 6 may be used. Formable porous materials, for example, nylons such as nylon 6,6 and nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof, or The absorptive region 4 of the biochemical analysis unit 1 can be formed by a porous carbon material such as activated carbon, and further, a metal such as platinum, gold, iron, silver, nickel, aluminum; alumina, silica, titania. , A metal oxide such as zeolite; a metal salt such as hydroxyapatite or calcium sulfate, an inorganic porous material such as a complex thereof, or a bundle of a plurality of fibers forms the adsorptive region 4 of the biochemical analysis unit 1. You may do it.

Further, in the above-mentioned embodiment, the biochemical analysis unit 1 is provided with the aluminum substrate 2, but it is not always necessary to form the substrate 2 of the biochemical analysis unit 1 from aluminum. Alternatively, the substrate 2 can be formed of another material. The material for forming the substrate 2 of the biochemical analysis unit 1 preferably has a property of attenuating radiation,
The biochemical analysis unit 1 is not particularly limited, and may be made of an inorganic compound material or an organic compound material.
The substrate 2 can also be formed, and a metal material, a ceramic material or a plastic material is preferably used, and a metal material is particularly preferably used from the viewpoint of having a high radiation attenuation ability. Examples of metal materials that can be preferably used to form the substrate 2 of the biochemical analysis unit 1 include gold, silver, copper, zinc, and the like.
Metals such as aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin, and selenium; alloys such as brass, stainless steel, and bronze. Examples of inorganic compound materials other than metal materials include silicon and amorphous. Silicon materials such as silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. Can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, as the organic compound material that can be used to form the substrate 2 of the biochemical analysis unit 1, a polymer compound is preferably used, and a preferable polymer compound is, for example, a polyolefin such as polyethylene or polypropylene. Acrylic resins such as polymethylmethacrylate and butylacrylate / methylmethacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyethylene naphthalate and Polyester such as polyethylene terephthalate; nylon such as nylon 6, nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; polydiphe Silicone resins such as siloxane; phenolic resins such as novolac; epoxy resins; polyurethane; polystyrene; butadiene-styrene copolymers; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; Examples include chitosan; sumac, polyamide such as gelatin, collagen, keratin, and copolymers of these polymer compounds. These may be composite materials, and if necessary, metal oxide particles, glass fibers and the like can be filled, and by blending an organic compound material,
It can also be used.

Further, in the above-mentioned embodiment, in the many absorptive regions 4 of the biochemical analysis unit 1, nylon 6 is filled inside the many through holes 3 formed in the substrate 2 made of aluminum. Although formed, the adsorptive film containing the adsorptive material is press-fitted into the multiple through holes 3 formed in the substrate 2 to form the multiple adsorptive regions 4 of the biochemical analysis unit 1. You can also

Further, in the above embodiment, the large number of absorptive regions 4 of the biochemical analysis unit 1 are obtained by filling the inside of the large number of through holes 3 formed in the aluminum substrate 2 with nylon 6. , Which is formed, has a property of attenuating radiation on at least one surface of the absorptive substrate formed of the absorptive material, and a substrate having a plurality of through-holes is adhered to the substrate to form a plurality of through-holes. It is also possible to form a plurality of absorptive regions separated from each other by the absorptive substrate in the holes.

[0345]

According to the present invention, it is possible to provide a hybridization bag which can automate hybridization and is easy to handle, a hybridization method and a hybridization apparatus using the same.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit carrying a specific binding substance to which a substance derived from a living body is hybridized by a hybridization device according to a preferred embodiment of the present invention.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic plan view of a hybridization bag according to a preferred embodiment of the present invention.

FIG. 4 is a schematic side view of a hybridization device according to a preferred embodiment of the present invention.

FIG. 5 is a block diagram showing a control system, a drive system and an input system of a hybridization apparatus according to a preferred embodiment of the present invention.

FIG. 6 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 7 shows a large number of stimulable phosphor layer regions formed on a stimulable phosphor sheet by a radiolabel substance contained in a large number of adsorptive regions formed in a biochemical analysis unit. It is a schematic sectional drawing which shows the method of exposing.

FIG. 8 is a schematic perspective view of a scanner.

FIG. 9 is a schematic perspective view showing details of a scanner near the photomultiplier.

10 is a schematic cross-sectional view taken along the line AA of FIG.

11 is a schematic cross-sectional view taken along the line BB of FIG.

FIG. 12 is a schematic cross-sectional view taken along the line CC of FIG.

FIG. 13 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 14 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 15 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner.

FIG. 16 is a schematic perspective view of a hybridization bag according to another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical analysis unit 2 substrates 3 through holes 4 Adsorbable area 5 injectors 6 CCD camera 7 hybridization bag 8 plastic sheets 8a, 8b Seal part 9 Holder 10 perforations 11a First solution injection part 11b Second solution injection part 12 endless belt 13 cutter 14 heater 15 Squeeze Roller 16 Pretreatment liquid pin 17 Hybridization solution injection pin 18 Probe solution injection pin 19 injection pin head 20 control unit 21 Endless belt motor 22 pin head motor 23a Pretreatment liquid pump 23b Hybridization solution pump 23c probe solution pump 24 keyboard 25 Accumulative phosphor sheet 26 Support 27 Photostimulable phosphor layer area 28 through holes 31 First Laser Excitation Light Source 32 Second laser excitation light source 33 Third Laser Excitation Light Source 34 Laser light 35 Collimator lens 36 mirror 37 First Dichroic Mirror 38 Second dichroic mirror 39 mirror 40 collimator lens 41 Collimator lens 42 mirror 43 Holes for Mirror 44 holed mirror 45 Optical head 46 mirror 47 Aspherical lens 48 concave mirror 50 stages 51 glass plate 55 Fluorescence or stimulated emission 58 Filter unit 60 Photomultiplier 61a, 61b, 61c, 61d Filter member 62a, 62b, 62c, 62d filters 63 A / D converter 64 data processor 70 board 71 Sub-scanning pulse motor 72 a pair of rails 73 Movable board 74 rod 75 Main scanning stepping motor 76 endless belt 77 Linear encoder 78 Linear encoder slit 80 control unit 81 keyboard 82 Filter unit motor 91a First solution injection part 91b Second solution injection part 91c Third solution injection part

Continued front page    F term (reference) 2G045 DA12 DA13 DA14 FB02 FB07                       FB12 GC15 HA14                 4B029 AA07 AA23 BB20 FA15                 4B063 QA01 QA17 QA18 QA19 QA20                       QQ42 QQ52 QR56 QS25 QS34                       QS39 QX02 QX07

Claims (23)

[Claims] 1. Formed from a plastic sheet,
A hybridization bag having one end sealed, along both edges, a pair of thick-walled holding portions extending in the longitudinal direction are formed, and in each of the pair of holding portions,
A hybridization bag, wherein perforations are formed along the longitudinal direction, and at least one solution injection part into which a pin for injecting a solution can be inserted is formed in one of the pair of holding parts. 2. The high according to claim 1, wherein the at least one solution injecting unit is configured to close a portion in which the pin is inserted after pulling out the inserted pin. Hybridization bag. 3. The hybridization bag according to claim 2, wherein the at least one solution injection part is formed of elastic rubber. 4. A solution injecting portion, into which a solution injecting pin can be inserted and which is spaced apart from each other in the longitudinal direction, is formed in one of the pair of holding portions. The hybridization bag according to any one of items. 5. The solution injection unit according to claim 1, wherein a pin for injecting a solution can be inserted into one of the pair of holding units, and three solution injection units spaced from each other in the longitudinal direction are formed. Hybridization bag. 6. The hybridization bag according to claim 1, wherein the plastic sheet is made of a thermoplastic resin. 7. Formed by a plastic sheet,
A hybridization bag having one end sealed, along both edges, a pair of thick-walled holding portions extending in the longitudinal direction are formed, and in each of the pair of holding portions,
A perforation is formed along the longitudinal direction, and at least one solution injection part capable of inserting a solution injection pin is formed in one of the pair of holding parts. A plurality of absorptive regions containing known specific binding substances accommodate biochemical analysis units formed apart from each other, seal the other end of the hybridization bag, and the hybridization bag, A drive mechanism is engaged with the perforations formed in the pair of holding parts, and the at least one solution injection part is
The hybridization solution injection pin is inserted, the hybridization solution is injected into the hybridization bag, the hybridization solution injection pin is pulled out from the at least one solution injection part, and the squeeze roller means is connected to the one end part. By sandwiching the hybridization bag between the at least one solution injecting section and driving the driving mechanism, the hybridization bag is moved with respect to the squeeze roller means, and The hybridization solution is agitated to perform prehybridization and at least 1
Insert the probe solution injection pin into the two solution injection parts,
A probe solution containing a substance derived from a living body labeled with a labeling substance is injected into the hybridization bag, and the probe solution injection pin is connected to the at least one
From one solution injecting section, and the squeeze roller means holds the hybridization bag between the one end and the at least one solution injecting section, drives the driving mechanism, and Move to the squeeze roller means,
A hybridization method, wherein a solution obtained by adding a probe solution to the hybridization solution in the hybridization bag is stirred to perform hybridization. 8. After performing the hybridization,
The vicinity of the other end of the hybridization bag is cut, and the squeeze roller means holds the hybridization bag between the one end and the at least one solution injection part, and drives the drive mechanism,
The hybridization method according to claim 7, wherein the hybridization bag is moved with respect to the squeeze roller means, and the solution in which the probe solution is added to the hybridization solution is discharged. 9. The at least one solution injection part formed in one of the pair of holding parts of the hybridization bag, after pulling out the hybridization solution injection pin and the probe solution injection pin inserted, The hybridization method according to claim 7 or 8, wherein a portion where the hybridization solution injection pin and the probe solution injection pin are inserted is closed. 10. The at least one formed on one of the pair of holding portions of the hybridization bag.
The hybridization method according to claim 9, wherein the one solution injection portion is formed of elastic rubber. 11. A first solution injection part and a second solution injection part, into which a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag and which are spaced apart from each other in the longitudinal direction. The above-mentioned hybridization bag accommodates a biochemical analysis unit in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are formed apart from each other, and the other end of the hybridization bag A portion of the first solution injection part and the second solution injection part of the hybridization bag, the driving mechanism is engaged with the perforations formed in the pair of holding parts. The hybridization solution is added to the second solution injection unit located distally from the one end of the hybridization bag. The hybridization solution is injected into the hybridization bag by inserting the injection pin, the hybridization solution injection pin is pulled out from the second solution injection section, and the hybridization bag is injected into the second solution. A portion near the one end side, and the squeeze roller means holds the hybridization bag and drives the driving mechanism to move the hybridization bag with respect to the squeeze roller means. Then, the hybridization solution in the hybridization bag is agitated to perform pre-hybridization, and the probe solution injection pin is inserted into the first solution injection part to insert the probe solution into the hybridization bag. Inject into the front The probe solution injection pin is pulled out from the first solution injection part, the hybridization bag is sealed in the vicinity of the one end side of the first solution injection part, and the hybridization bag is attached to the squeeze roller means. And driving the drive mechanism to move the hybridization bag with respect to the squeeze roller means, and agitate the solution in which the probe solution in the hybridization bag is added to the hybridization solution. The hybridization method according to claim 7, wherein the hybridization is performed. 12. A first solution injection part, into which a pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and which is separated from each other in the longitudinal direction,
A plurality of absorptive regions containing a specific binding substance having a known structure or characteristic are formed in the hybridization bag in which the second solution injection part and the third solution injection part are formed, separated from each other. The chemical analysis unit is housed, the other end of the hybridization bag is sealed, and the hybridization bag is engaged with the driving mechanism to the perforations formed in the pair of holding portions, and the first Solution injection part of the second
Of the solution injecting part and the third solution injecting part, the pretreatment liquid injection pin is inserted into the third solution injecting part located farthest from the one end of the hybridization bag, and the pretreatment is performed. A liquid is injected into the hybridization bag, the pretreatment liquid injection pin is pulled out from the third solution injection part, and the hybridization bag is near the one end side of the third solution injection part. Then, the squeeze roller means is sandwiched, the hybridization bag is sandwiched, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means. The pretreatment solution is stirred to perform pretreatment, and the hybridization bag is
In the vicinity of the one end side of the third solution injection part, cutting is performed, the pretreatment liquid is discharged, and the hybridization solution injection pin is inserted into the second solution injection part to remove the hybridization solution. , Injecting into the hybridization bag, pulling out the hybridization solution injection pin from the first solution injection part, the hybridization bag in the vicinity of the one end side of the second solution injection part, After sealing, the squeeze roller means holds the hybridization bag, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means, and the hybridization bag inside the hybridization bag is moved. Perform pre-hybridization by stirring the hybridization solution The probe solution injection pin is inserted into the first solution injection part, the probe solution is injected into the hybridization bag, and the probe solution injection pin is extracted from the first solution injection part. The hybridization bag is sealed in the vicinity of the one end side of the first solution injecting section, the squeeze roller means holds the hybridization bag, and the driving mechanism is driven to drive the hybridization bag. Is moved with respect to the squeeze roller means, and the solution in which the probe solution in the hybridization bag is added to the hybridization solution is stirred to perform the hybridization. 9. The hybridization method according to item 8. 13. A pin for injecting a solution can be inserted into one of the pair of holding parts of the hybridization bag, and a first solution injecting part and a second solution injecting part which are separated from each other in the longitudinal direction are formed. The above-mentioned hybridization bag accommodates a biochemical analysis unit in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are formed apart from each other, and the other end of the hybridization bag A portion of the first solution injection part and the second solution injection part of the hybridization bag, the driving mechanism is engaged with the perforations formed in the pair of holding parts.
A pretreatment liquid injection pin is inserted into the second solution injection portion located distally from the one end of the hybridization bag, and the pretreatment liquid is injected into the hybridization bag. The injection pin is pulled out from the second solution injection portion, and the squeeze roller means,
The hybridization bag is sandwiched between the one end portion and the second solution injecting section, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means to move the hybridization bag. The pretreatment liquid in the hybridization bag is agitated to perform the pretreatment, and the hybridization bag is cut near the one end side of the second solution injection part, and the pretreatment liquid is discharged. The hybridization solution injection pin is inserted into the first solution injection part, the hybridization solution is injected into the hybridization bag, and the hybridization solution injection pin is inserted from the first solution injection part. Withdrawing the squeeze roller means, the hybrid between the one end and the first solution injecting section is removed. The homogenization bag is sandwiched,
The driving mechanism is driven, the hybridization bag is moved with respect to the squeeze roller means, the hybridization solution in the hybridization bag is agitated, and pre-hybridization is performed. In the solution injection part, the probe solution injection pin is inserted, the probe solution is injected into the hybridization bag, the probe solution injection pin is extracted from the first solution injection part, the squeeze roller means, The hybridization bag is sandwiched between the one end portion and the first solution injecting portion, the driving mechanism is driven, and the hybridization bag is moved with respect to the squeeze roller means to move the hybridization bag. The probe solution in the hybridization bag The added solution internalization solution was stirred and hybridization method according to claim 7 or 8, characterized in that cheat perform hybridization. 14. The at least one formed on one of the pair of holding portions of the hybridization bag.
Two solution injection parts, the pretreatment liquid injection pin inserted,
After the hybridization solution injection pin and the probe solution injection pin are pulled out, the pretreatment solution injection pin, the hybridization solution injection pin and the probe solution injection pin are configured to be closed. The hybridization method according to claim 13, which is characterized in that 15. The hybridization method according to claim 7, wherein the plastic sheet is formed of a thermoplastic and the hybridization bag is sealed by heat sealing. 16. The substance derived from a living body is labeled with at least one labeling substance selected from the group consisting of a radioactive labeling substance, a fluorescent substance, and a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate. The hybridization method according to any one of claims 7 to 15, wherein: 17. The biochemical analysis unit comprises a substrate having a plurality of holes formed therein, and the plurality of absorptive regions are provided.
17. The hybridization method according to claim 7, wherein the plurality of holes of the substrate are formed by filling an adsorptive material. 18. The biochemical analysis unit includes an absorptive substrate formed of an absorptive material, and at least one surface of the absorptive substrate has a property of attenuating radiation and has a plurality of through holes. Adhere the substrate with the
17. The plurality of absorptive regions are formed by containing the specific binding substance in the absorptive substrate in the plurality of through holes formed in the substrate. The method of hybridization according to item 1. 19. The hybridization method according to claim 17, wherein the substrate of the biochemical analysis unit has a property of attenuating radiation. 20. When the radiation of the substrate of the biochemical analysis unit is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1/5. 20. The hybridization method according to claim 19, which has the following property of attenuating. 21. The biochemical analysis unit comprises an absorptive substrate formed of an absorptive material, and the plurality of absorptive regions are specific binding substances whose structures or characteristics are known to the absorptive substrate. The hybridization method according to any one of claims 7 to 16, which is formed by dropping a solution containing 22. A drive mechanism capable of conveying the hybridization bag in both forward and reverse directions by engaging with perforations formed in a pair of thick-walled holding portions formed along both edges of the hybridization bag. And a hybridization solution injection pin configured to inject a hybridization bag solution into the hybridization bag, and a probe solution containing a biological substance labeled with a labeling substance into the hybridization bag A probe solution injection pin configured as described above, and a sealing heater configured to heat seal the hybridization bag,
A cutter for cutting the hybridization bag in the width direction and a squeeze roller means configured to be able to sandwich the hybridization bag are provided, and the hybridization solution injection pin and the probe solution injection pin are the same as those of the hybridization bag. A hybridization device, wherein the hybridization device is configured to be movable between an insertion position inserted into a solution injection part formed in the pair of holding parts and a retracted position separated from the solution injection part. 23. Further, a pretreatment liquid injection pin for injecting a pretreatment liquid is provided in the hybridization bag, and the pretreatment liquid injection pin is formed in the pair of holding portions of the hybridization bag. 23. The hybridization device according to claim 22, wherein the hybridization device is configured to be movable between an insertion position inserted into the solution injection unit and a retracted position separated from the solution injection unit.