JP2003038162A - Sampler for testing bacteria - Google Patents
Sampler for testing bacteriaInfo
- Publication number
- JP2003038162A JP2003038162A JP2002146443A JP2002146443A JP2003038162A JP 2003038162 A JP2003038162 A JP 2003038162A JP 2002146443 A JP2002146443 A JP 2002146443A JP 2002146443 A JP2002146443 A JP 2002146443A JP 2003038162 A JP2003038162 A JP 2003038162A
- Authority
- JP
- Japan
- Prior art keywords
- filter
- sample
- bacteria
- container
- tubular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、飲料水や液体食品
などの液体中の細菌などを検出するために用いられる細
菌検査用サンプル収集装置に関し、より詳細には、フィ
ルタに捕捉された細菌を検出することにより細菌を検査
するのに用いられる細菌検査用サンプル収集装置に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample collecting apparatus for bacterial tests used for detecting bacteria in liquids such as drinking water and liquid foods, and more particularly to bacteria collecting in a filter. The present invention relates to a sample collecting device for bacterial test, which is used for testing bacteria by detecting.
【０００２】[0002]
【従来の技術】従来、液体中の細菌検査に際しては、液
体の検体をスポイドなどを用いて採取し、採取された検
体を培養することにより細菌の数を増加させ、しかる後
検体中の細菌の数を測定する方法が用いられていた。し
かしながら、このような方法では、細菌を培養する必要
があるため測定までに長時間を要する。また、検体中に
濁りを含む成分が含まれていることもあるため、このよ
うな濁りの原因となる成分により測定精度が低下すると
いう問題があった。2. Description of the Related Art Conventionally, in the case of testing bacteria in a liquid, a liquid sample is collected by using a spoid, and the number of bacteria is increased by culturing the collected sample. The method of measuring the number was used. However, in such a method, it takes a long time to perform the measurement because it is necessary to culture the bacteria. Further, since the sample may contain a component containing turbidity, there is a problem that the measurement accuracy is lowered by the component causing such turbidity.
【０００３】他方、「食品と開発Ｖｏｌ．３６，Ｎｏ
１、３２頁〜３８頁（２００１）」には、液体の検体か
ら細菌を検出するための様々な装置が説明されている。
すなわち、上記のように、液体の検体を採取した後、培
養し、しかる後細菌の数を検出するための各種キット、
あるいは培養操作を必要とせずに、直接細菌を測定する
方法などが示されている。しかしながら、前者の培養操
作を必要とするキットでは、前述したように測定に長時
間を必要とする。また、後者の測定方法としては、細菌
の電気抵抗を利用したインピーダンス法や、細胞内のＡ
ＴＰを指標としたバイオルミネッセンス法、メンブレン
フィルタ上で捕捉された細菌をＡＴＰ発光試薬で標識し
て測るメンブレン・フォトン検出法などが開示されてい
る。On the other hand, "Food and Development Vol. 36, No.
1, pages 32 to 38 (2001) "describes various devices for detecting bacteria in a liquid sample.
That is, as described above, after collecting a liquid sample, culturing, then various kits for detecting the number of bacteria,
Alternatively, a method for directly measuring bacteria without the need for culturing operation is shown. However, in the case of the former kit requiring the culture operation, the measurement requires a long time as described above. In addition, as the latter measuring method, an impedance method utilizing the electric resistance of bacteria, or intracellular A
A bioluminescence method using TP as an index, a membrane photon detection method in which bacteria captured on a membrane filter are labeled with an ATP luminescent reagent and measured, and the like are disclosed.
【０００４】しかしながら、これらの方法においても、
液体の検体から菌を採取し、測定するまでの操作が煩雑
であり、一般人が液体の検体から細菌を高精度に測定す
ることは困難であった。例えば、メンブレンフィルタ上
で捕捉された菌をＡＴＰ発光試薬で標識し測定するメン
ブレン・フォトン検出法などでは、液体試料をスポイド
などにより採取した後、フィルタで濾過し、しかる後細
菌数測定装置によりフィルタ上に捕捉された細菌を測定
する必要がある。従って、スポイドや注射器などにより
液体試料を採取する作業、フィルタにより液体の検体を
濾過する作業などが煩雑であった。However, even in these methods,
The operation of collecting and measuring the bacteria from the liquid sample is complicated, and it is difficult for the general public to measure the bacteria with high accuracy from the liquid sample. For example, in the membrane photon detection method in which bacteria captured on a membrane filter are labeled with an ATP luminescent reagent and measured, a liquid sample is collected by a spoid or the like, then filtered by a filter, and then filtered by a bacteria count measuring device. It is necessary to measure the bacteria trapped above. Therefore, the work of collecting a liquid sample with a spoid or a syringe, the work of filtering a liquid sample with a filter, and the like are complicated.
【０００５】[0005]
【発明が解決しようとする課題】本発明の目的は、上述
した従来技術の現状に鑑み、液体の検体中の細菌を簡便
な操作で容易に収集することを可能とする細菌検査用サ
ンプル収集装置を提供することにある。SUMMARY OF THE INVENTION In view of the above-mentioned state of the art, an object of the present invention is to provide a sample collecting apparatus for bacterial test which enables bacteria in a liquid sample to be easily collected by a simple operation. To provide.
【０００６】[0006]
【課題を解決するための手段】本発明に係る細菌検査用
サンプル収集装置は、液体を検体とする細菌検査に際し
サンプルを収集するための細菌検査用サンプル収集装置
であって、検体が注がれる開口と、前記開口から注がれ
た検体を濾過するとともに、検体に含まれていた細菌を
捕捉するためのフィルタが取り付けられるフィルタ取付
部と、前記フィルタで濾過された検体が排出される排出
空間とを有し、前記排出空間に連通されておりかつ前記
フィルタから遠ざかる方向に先端が延ばされた中空針と
を備えるハウジングと、前記ハウジングのフィルタ取付
部に取り付けられたフィルタとを有する細菌収集器具
と、内部が減圧されておりかつ前記中空針により穿刺さ
れ得る栓体により密封されている液体収納容器とを備え
ることを特徴とする。A sample collecting device for bacterial test according to the present invention is a sample collecting device for bacterial test for collecting a sample in a bacterial test using a liquid as a sample, to which a sample is poured. An opening, a filter attachment part for filtering the sample poured from the opening, and a filter for attaching the bacteria contained in the sample, and a discharge space for discharging the sample filtered by the filter And a housing having a hollow needle that is in communication with the discharge space and has a distal end extended in a direction away from the filter, and a filter attached to a filter mounting portion of the housing. An instrument and a liquid storage container, the inside of which is depressurized and which is sealed by a plug body that can be punctured by the hollow needle, are provided.
【０００７】本発明の細菌検査用サンプル収集装置の特
定の局面では、前記ハウジングが、上端に検体が注がれ
る前記開口を有し、下端が開放されている略筒状の第１
の筒状容器と、前記第１の筒状容器の下方部分に嵌め込
まれるように構成されており、かつ上面に前記フィルタ
取付部が設けられた略筒状の第２の筒状容器と、前記第
２の筒状容器の下方から第２の筒状容器に嵌め込まれ
て、第２の筒状容器とともに前記排出空間を構成してお
り、かつ該排出空間に連通しており、かつ先端が下方に
延ばされた前記中空針が設けられた第３の筒状容器とを
備える。[0007] In a specific aspect of the sample collecting apparatus for bacteria test of the present invention, the housing has the opening having the upper end at which the specimen is poured, and the lower end is open, and is substantially cylindrical in shape.
A tubular container, and a substantially tubular second tubular container configured to be fitted into a lower portion of the first tubular container and provided with the filter mounting portion on an upper surface thereof. The second tubular container is fitted into the second tubular container from below, constitutes the discharge space together with the second tubular container, and communicates with the discharge space, and the tip is downward. And a third cylindrical container provided with the hollow needle extended to the.
【０００８】この第３の減圧にされた筒状容器によって
吸引ろ過し得るため、任意の時にどのような場所におい
ても、他の器具の助けを借りないで簡便に測定すること
ができる。[0008] Since suction filtration can be performed by the third depressurized cylindrical container, it is possible to easily perform measurement at any place at any time without the aid of other instruments.
【０００９】本発明に係る細菌検査用サンプル収集装置
の他の特定の局面では、上記第１の筒状容器の中間高さ
位置に、検体中の塵埃を除去するための第２のフィルタ
が取り付けられており、それによって、検体中の塵埃や
濁りを発生する成分などを除去することができ、より高
精度に細菌を検査することを可能とするサンプルを収集
することができる。In another specific aspect of the sample collecting apparatus for bacterial test according to the present invention, a second filter for removing dust in a sample is attached to an intermediate height position of the first cylindrical container. As a result, it is possible to remove dust, turbidity-producing components, and the like in the sample, and collect a sample that enables more accurate testing of bacteria.
【００１０】[0010]
【発明の実施の形態】以下、本発明の具体的な実施形態
を説明することにより、本発明をより詳細に説明する。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail below by describing specific embodiments of the present invention.
【００１１】図１は、本発明の一実施形態に係る細菌検
査用サンプル収集装置の縦断面図であり、図２はその分
解された状態を示す縦断面図である。細菌検査用サンプ
ル収集装置１は、細菌収集器具２と、液体収納容器１１
とを備える。FIG. 1 is a vertical sectional view of a sample collecting apparatus for bacterial test according to an embodiment of the present invention, and FIG. 2 is a vertical sectional view showing its disassembled state. The sample collecting apparatus 1 for bacteria test includes a bacteria collecting instrument 2 and a liquid storage container 11
With.
【００１２】細菌収集器具２は、第１の筒状容器３と、
第２の筒状容器４と、第３の筒状容器５とを備える。第
１の筒状容器３は、上方に開口６ａを有する略円筒状の
筒状部材６と、筒状部材６の下端に外折される上方開口
７ａを有する略円筒状の第２の筒状部材７とを組み合わ
せることにより構成されている。筒状部材６では、開口
６ａ側に向かってすなわち上方にいくにつれて拡がる形
状とされており、それによって液体の検体を容易に筒状
部材６内に注ぐことが可能とされている。The bacteria collecting device 2 comprises a first cylindrical container 3 and
A second tubular container 4 and a third tubular container 5 are provided. The first cylindrical container 3 has a substantially cylindrical cylindrical member 6 having an opening 6a at the upper side, and a substantially cylindrical second cylindrical shape having an upper opening 7a outwardly bent at the lower end of the cylindrical member 6. It is configured by combining with the member 7. The tubular member 6 has a shape that widens toward the opening 6a side, that is, as it goes upward, so that the liquid sample can be easily poured into the tubular member 6.
【００１３】図３（ａ）〜（ｃ）に示されているよう
に、筒状部材６の下面には、複数の貫通口６ｂが形成さ
れている。複数の貫通口６ｂは、液体の検体を下方に流
下させるために設けられている。As shown in FIGS. 3 (a) to 3 (c), a plurality of through holes 6b are formed on the lower surface of the tubular member 6. The plurality of through holes 6b are provided to allow the liquid sample to flow downward.
【００１４】なお、本実施形態では、複数の貫通口６ｂ
が複数のリブ６ｃにより隔てられているが、このような
リブ６ｃを設けることなく、下面に多数の貫通口を形成
した構造であってもよい。In this embodiment, a plurality of through holes 6b are provided.
Are separated by a plurality of ribs 6c, but a structure in which a large number of through holes are formed on the lower surface may be used without providing such ribs 6c.
【００１５】他方、筒状部材７は、図４（ａ）及び
（ｂ）に示すように、上方に開いた開口７ａ及び下方に
開いた開口７ｂを有する。また、筒状部材７の中間高さ
位置には、複数の貫通口７ｃを有するフィルタ載置部７
ｄが設けられている。フィルタ載置部７ｄ上に、本発明
における第２のフィルタ８が載置される。第２のフィル
タ８は、液体の検体中の塵埃などを除去するために用い
られる。この第２のフィルタ８は本発明において必ずし
も必須ではないが、液体の検体中の塵埃や汚染成分を除
去することにより、測定をより高精度に行うことができ
る。第２のフィルタ８を構成する材料としては、例え
ば、ミリポア社製、メンブレンフィルタ品番：ＭＩＬＬ
ＥＸ（ポアサイズ０．５μｍ）などの適宜の塵埃を除去
し得るフィルタ材料を用いることができる。On the other hand, the tubular member 7 has an opening 7a opened upward and an opening 7b opened downward, as shown in FIGS. 4 (a) and 4 (b). Further, the filter mounting portion 7 having a plurality of through holes 7c is provided at the intermediate height position of the tubular member 7.
d is provided. The second filter 8 of the present invention is placed on the filter placement portion 7d. The second filter 8 is used to remove dust and the like in the liquid sample. The second filter 8 is not essential in the present invention, but the measurement can be performed with higher accuracy by removing dust and contaminant components in the liquid sample. As a material forming the second filter 8, for example, a membrane filter manufactured by Millipore, product number: MILL
A filter material capable of removing appropriate dust such as EX (pore size 0.5 μm) can be used.
【００１６】第２のフィルタ８は、フィルタ載置部７ｄ
上に配置され、筒状部材７に筒状部材６を組み合わせた
時に、すなわち筒状部材６の下面がフィルタ８に当接さ
れるように筒状部材６を筒状部材７の開口７ａに挿入
し、組み立てることにより第１の筒状容器３が構成され
る。The second filter 8 has a filter mounting portion 7d.
The tubular member 6 is inserted into the opening 7a of the tubular member 7 when the tubular member 6 is arranged on the upper side, that is, the lower surface of the tubular member 6 contacts the filter 8 when the tubular member 6 is combined with the tubular member 7. Then, the first cylindrical container 3 is constructed by assembling.
【００１７】従って、第１の筒状容器３においては、上
記第２のフィルタ８が中間高さ位置に配置されているこ
とになる。他方、第１の筒状容器３の筒状部材７の下方
部分においては、内周面に雌ねじ７ｅが形成されてい
る。この雌ねじ７ｅを利用して第２の筒状容器４がねじ
込まれるように、第２の筒状容器４の外周面に雄ねじ４
ａが形成されている。また、第２の筒状容器４の上面に
は、フィルタ取付部材９が取り付けられる。フィルタ取
付部材９は、細菌を捕捉するためのフィルタ１０を第２
の筒状容器４に取り付けるために設けられている。Therefore, in the first cylindrical container 3, the second filter 8 is arranged at the intermediate height position. On the other hand, a female screw 7e is formed on the inner peripheral surface of the lower portion of the tubular member 7 of the first tubular container 3. The male screw 4 is attached to the outer peripheral surface of the second tubular container 4 so that the second tubular container 4 can be screwed in using the female screw 7e.
a is formed. A filter attachment member 9 is attached to the upper surface of the second tubular container 4. The filter mounting member 9 has a second filter 10 for capturing bacteria.
It is provided to be attached to the cylindrical container 4.
【００１８】図５（ａ）〜（ｃ）に示すように、本実施
形態では、フィルタ取付部材９は、中心に円形の開口９
ａを有するドーナツ型の平板状部材９ｂを有する。この
平板状部材９ｂの下面には、下方に延びる複数の突起９
ｃが設けられている。図５に拡大して示すように、突起
９ｃでは、その先端にいくにつれて突起９ｃの幅が狭く
なるように先端側に傾斜面９ｃ1が形成されており、傾
斜面９ｃ1の後端に、係止部９ｃ2が設けられている。係
止部９ｃ2は、突起９ｃの上端側部分よりも側方に突出
するように形成されている。As shown in FIGS. 5A to 5C, in this embodiment, the filter mounting member 9 has a circular opening 9 at the center.
It has a donut-shaped flat member 9b having a. A plurality of projections 9 extending downward are provided on the lower surface of the flat plate-shaped member 9b.
c is provided. As shown in an enlarged manner in FIG. 5, in the projection 9c, an inclined surface 9c 1 is formed on the tip side so that the width of the projection 9c becomes narrower toward the tip, and at the rear end of the inclined surface 9c 1 , A locking portion 9c 2 is provided. The locking portion 9c 2 is formed so as to project laterally beyond the upper end side portion of the projection 9c.
【００１９】他方、図７に示すように第２の筒状容器４
では、上面に、複数の貫通口４ｂが形成されている。複
数の貫通口４ｂは、上記フィルタ取付部材９の突起９ｃ
を係止するために設けられている。すなわち、貫通口４
ｂは、突起９ｃの係止部９ｃ 2が設けられている部分の
径よりも若干小さい径の小径部４ｂ1と、小径部よりも
径が大きく、小径部４ｂ1の下方に連ねられた大径部４
ｂ2とを有する。従って、突起９ｃを貫通口４ａに外力
を加えて挿入した後には、小径部４ｂ1と係止部９ｃ2と
が設けられており、係止部９ｃ2が小径部４ｂ1と大径部
４ｂ2との段差により係止されることにより、突起９ｃ
が貫通口４ａから上方に移動されないように構成されて
いる。On the other hand, as shown in FIG. 7, the second cylindrical container 4
Then, a plurality of through-holes 4b are formed on the upper surface. Compound
The number of through-holes 4b are the projections 9c of the filter mounting member 9.
It is provided to lock the. That is, the through hole 4
b is a locking portion 9c of the protrusion 9c 2Of the part where
Small diameter part 4b with a diameter slightly smaller than the diameter1Than the small diameter part
Large diameter, small diameter part 4b1Large diameter part 4 connected below
b2Have and. Therefore, the protrusion 9c is applied to the through hole 4a by an external force.
After adding and inserting the small diameter portion 4b1And locking part 9c2When
Is provided, and the locking portion 9c is provided.2Is the small diameter part 4b1And large diameter part
4b2And the protrusion 9c
Is configured so as not to move upward from the through hole 4a.
There is.
【００２０】他方、上記のようにして、複数の突起９ｃ
を複数の貫通口４ａに挿入することにより、フィルタ取
付部材９が第２の筒状容器４の上面に固定されるが、こ
の固定に際し、フィルタ取付部材９の下面と第２の筒状
容器４との間に上述したフィルタ１０を介在させる。On the other hand, as described above, the plurality of protrusions 9c
The filter mounting member 9 is fixed to the upper surface of the second cylindrical container 4 by inserting the plurality of through holes 4a into the through hole 4a. At the time of this fixing, the lower surface of the filter mounting member 9 and the second cylindrical container 4 are fixed. The filter 10 described above is interposed between the above and.
【００２１】フィルタ１０としては、目的とする細菌を
捕捉し得る適宜のフィルタ材料を用いることができ、よ
り具体的には、０．１〜０．８μｍの径の微細な穴が多
数設けられているフィルタ材料、例えば、東洋濾紙社
製、アドバンテックフィルタ（ポアサイズ０．２μｍ）
を用いることができる。As the filter 10, an appropriate filter material capable of capturing desired bacteria can be used, and more specifically, a large number of fine holes having a diameter of 0.1 to 0.8 μm are provided. Filter material, such as Toyo Roshi Kaisha's Advantech filter (pore size 0.2 μm)
Can be used.
【００２２】第２の筒状容器４の下方には、開口４ｃが
設けられており、該開口４ｃの内周面に雌ねじ４ｄが形
成されている。また、第２の筒状容器４の天板中央には
複数の貫通孔４ｅが検体を下方に沈下するために形成さ
れている。An opening 4c is provided below the second cylindrical container 4, and an internal thread 4d is formed on the inner peripheral surface of the opening 4c. In addition, a plurality of through holes 4e are formed in the center of the top plate of the second cylindrical container 4 to sink the specimen downward.
【００２３】上記雌ねじ４ｄを利用して第２の筒状容器
４の開口４ｃに第３の筒状容器５がねじ込まれるよう
に、第３の筒状容器５の上方の外周側面に雄ねじ５ａが
形成されている（図８参照）。図８（ａ），（ｂ）に示
すように、第３の筒状容器５では、上面に閉環状突起５
ｂが形成されている。この閉環状突起５ｂは、第３の筒
状容器５を第２の筒状容器４に上記のようにねじ込み、
組み合わせた場合に、閉環状突起５ｂの上面が第２の筒
状容器４の天板の下面に当接するように設けられてい
る。従って、第２の筒状容器４の下面と閉環状突起５ｂ
と第３の筒状容器５の上面とで囲まれた空間が、排出空
間Ａとして形成される。また、上記閉環状突起５ｂに囲
まれた中心部は、下方に延ばされた中空針５ｃに連ねら
れており、すなわち排出空間Ａに中空針５ｃが連通され
ている。中空針５ｃは内部に液体の流路を有し、該流路
は中空針５ｃの下方に設けられた開口５ｄに通じてい
る。A male screw 5a is provided on the outer peripheral side surface above the third tubular container 5 so that the third tubular container 5 can be screwed into the opening 4c of the second tubular container 4 using the female screw 4d. Is formed (see FIG. 8). As shown in FIGS. 8A and 8B, in the third cylindrical container 5, the closed annular protrusion 5 is formed on the upper surface.
b is formed. The closed annular projection 5b is formed by screwing the third tubular container 5 into the second tubular container 4 as described above.
When combined, the upper surface of the closed annular projection 5b is provided so as to contact the lower surface of the top plate of the second tubular container 4. Therefore, the lower surface of the second cylindrical container 4 and the closed annular protrusion 5b.
A space surrounded by and the upper surface of the third cylindrical container 5 is formed as a discharge space A. The central portion surrounded by the closed annular projection 5b is connected to the hollow needle 5c extending downward, that is, the hollow needle 5c is connected to the discharge space A. The hollow needle 5c has a liquid flow passage therein, and the flow passage communicates with an opening 5d provided below the hollow needle 5c.
【００２４】なお、上記雄ねじ５ａが形成されている部
分よりも下方では、第３の筒状容器５は略円筒状の形状
を有し、中空針５ｃよりも下方に円筒状部５ｅが延ばさ
れている。Below the portion where the male screw 5a is formed, the third cylindrical container 5 has a substantially cylindrical shape, and the cylindrical portion 5e extends below the hollow needle 5c. Has been done.
【００２５】上述した第１の筒状容器３、第２の筒状容
器４及び第３の筒状容器５が上記のようにして組み立て
られて、本発明におけるハウジングが構成される。な
お、上記ハウジングは、合成樹脂や金属などの適宜の材
料で構成され得る。The first cylindrical container 3, the second cylindrical container 4 and the third cylindrical container 5 described above are assembled as described above to form the housing of the present invention. The housing may be made of an appropriate material such as synthetic resin or metal.
【００２６】もっとも、中空針５ｃについては、後述の
液体収納容器１１の栓体１３を貫通し得るように、金属
などの剛性を有する材料で構成することが望ましい。図
１，図２に示すように、液体収納容器１１は、有底の容
器本体１２と、栓体１３とを有する。容器本体１２は、
ガラス、合成樹脂などの適宜の材料で構成され、好まし
くは透明性を有する材料で構成される。Of course, the hollow needle 5c is preferably made of a rigid material such as metal so that it can penetrate the plug 13 of the liquid container 11 described later. As shown in FIGS. 1 and 2, the liquid storage container 11 has a bottomed container body 12 and a plug 13. The container body 12 is
It is made of an appropriate material such as glass or synthetic resin, and is preferably made of a transparent material.
【００２７】容器本体１２の開口が栓体１３により閉栓
されている。栓体１３は、液体収納容器１１内を密封し
得るかぎり、ゴムまたはエラストマーなどの適宜の弾性
材料により構成され得る。The opening of the container body 12 is closed by a plug 13. The stopper 13 may be made of a suitable elastic material such as rubber or elastomer as long as it can seal the inside of the liquid container 11.
【００２８】また、液体収納容器１１は、内部が減圧さ
れている。これは、液体の検体を減圧度を利用して液体
収納容器１３に吸引し、それによってフィルタ８，１０
による検体の濾過を速やかに行うためである。上記液体
収納容器１１としては、例えば、市販の真空採血管など
を用いることができる。すなわち、例えば、積水化学工
業社製、商品名：インセパックやテルモ社製、商品名：
ベノジェクトIIなどを用いることができる。The inside of the liquid container 11 is depressurized. This sucks the liquid sample into the liquid storage container 13 by utilizing the degree of pressure reduction, and thereby the filters 8 and 10 are sucked.
This is because the sample is rapidly filtered by. As the liquid storage container 11, for example, a commercially available vacuum blood collection tube can be used. That is, for example, Sekisui Chemical Co., Ltd. product name: Insepack and Terumo product name:
Benoject II or the like can be used.
【００２９】次に、本実施形態の細菌検査用サンプル収
集装置の使用方法を説明する。使用に際しては、上記ハ
ウジングの第１の筒状容器３の開口６ａから液体の試料
を注ぎ込む。しかる後、中空針５ｃを液体収納容器１１
の栓体１３に穿刺し、貫通させる。その結果、中空針５
ｃの開口５ｄが液体収納容器１１内に位置し、液体収納
容器の減圧度により、液体の検体が上方から下方へ吸引
される。そのため、検体中の塵埃がフィルタ８により除
去され、かつ塵埃等が除去された液体の検体がフィルタ
１０により濾過され、フィルタ１０において細菌が捕捉
される。濾過された液体は、中空針５ｃを通り、液体収
納容器１１内に収納される。Next, a method of using the sample collecting apparatus for bacterial test of this embodiment will be described. In use, a liquid sample is poured from the opening 6a of the first cylindrical container 3 of the housing. Then, the hollow needle 5c is attached to the liquid storage container 11
The plug body 13 is punctured and penetrated. As a result, the hollow needle 5
The opening 5d of c is located in the liquid storage container 11, and the liquid sample is sucked downward from above due to the degree of pressure reduction of the liquid storage container. Therefore, the dust in the sample is removed by the filter 8, the liquid sample from which the dust and the like are removed is filtered by the filter 10, and the bacteria are captured by the filter 10. The filtered liquid passes through the hollow needle 5c and is stored in the liquid storage container 11.
【００３０】従って、本実施形態の細菌検査用サンプル
収集装置を用いれば、大きな開口６ａから液体の検体を
注ぎ込み、中空針５ｃを液体収納容器１１の栓体１３に
突き刺すだけで、容易にフィルタ１０上に細菌を捕捉す
ることができる。Therefore, if the sample collecting apparatus for bacterial test of this embodiment is used, the liquid sample is poured from the large opening 6a and the hollow needle 5c is pierced into the plug body 13 of the liquid storage container 11 to easily filter the filter 10. Bacteria can be trapped on top.
【００３１】よって、検査に際しては、上記のようにし
てフィルタ１０に細菌を捕捉した後に、ハウジングを分
解し、フィルタ１０を取り出し、細菌測定装置に供する
ことにより細菌数を容易に測定することができる。この
場合、上記ハウジングの分解は、第２の筒状容器４を第
１の筒状容器３に対して回転させるだけで容易に行うこ
とができる。また、測定に際しては、上記第２の筒状容
器４にフィルタ取付部材９によりフィルタ１０が取付ら
れている構造のまま、測定装置に適用してもよい。Therefore, at the time of inspection, the number of bacteria can be easily measured by capturing the bacteria in the filter 10 as described above, disassembling the housing, taking out the filter 10 and providing it to the bacteria measuring device. . In this case, the housing can be easily disassembled by simply rotating the second cylindrical container 4 with respect to the first cylindrical container 3. Further, at the time of measurement, the structure in which the filter 10 is attached to the second cylindrical container 4 by the filter attaching member 9 may be applied to the measuring device as it is.
【００３２】本願発明者の実験によれば、上記実施形態
の細菌検査用サンプル収集装置を用い、河川水１ｍＬを
筒状容器６の開口６ａから注ぎ込み、フィルタ９におい
て細菌を捕捉した後、該フィルタ９上の細菌数を、ミリ
ポア社製、微生物検出装置、商品名：ＭｉｃｒｏＳｔａ
ｒ−ＲＭＤＳを用いて測定したところ、１０4の菌数を
確認することができた。すなわち、本実施形態の細菌検
査用サンプル収集装置を用い、上記のように簡便な操作
で細菌がフィルタに捕捉され、かつ捕捉された細菌数を
容易に測定し得ることがわかる。According to an experiment conducted by the inventor of the present application, 1 mL of river water was poured from the opening 6a of the cylindrical container 6 using the sample collecting apparatus for bacterial test of the above-mentioned embodiment, and after the bacteria were captured by the filter 9, the filter was filtered. The number of bacteria on 9 is a microbe detector manufactured by Millipore, trade name: MicroSta
As a result of measurement using r-RMDS, it was possible to confirm a bacterial count of 10 4 . That is, it is understood that the bacteria are collected by the filter and the number of the collected bacteria can be easily measured by the simple operation as described above using the sample collecting apparatus for bacteria inspection of the present embodiment.
【００３３】[0033]
【発明の効果】本発明に係る細菌検査用サンプル収集装
置では、ハウジングが検体が注がれる開口を有し、該開
口から液体の検体を容易に注ぐことができる。また、上
記ハウジングの中空針を液体収納容器の栓体に穿刺し、
液体収納容器とハウジングの帯出空間を連通することに
より、液体収納容器の減圧度を利用して、液体の検体が
フィルタにおいて速やかに濾過され、細菌がフィルタに
確実に捕捉される。従って、非常に簡便な操作で、液体
の検体から細菌検査用サンプルを収集することができ
る。In the sample collecting apparatus for bacterial test according to the present invention, the housing has the opening through which the sample is poured, and the liquid sample can be easily poured from the opening. Further, the hollow needle of the housing is punctured into the stopper of the liquid storage container,
By making the liquid storage container and the housing space of the housing communicate with each other, by utilizing the degree of pressure reduction of the liquid storage container, the liquid sample is quickly filtered by the filter and the bacteria are reliably captured by the filter. Therefore, it is possible to collect a sample for bacterial test from a liquid specimen by a very simple operation.
【００３４】上記ハウジングが、第１〜第３の筒状容器
とを備える場合には、細菌をフィルタに捕捉した後、第
２の筒状容器を第１の筒状容器から取り外すだけで、細
菌が捕捉されたフィルタを容易に取り出すことができ
る。When the housing is provided with the first to third cylindrical containers, the bacteria can be captured by the filter, and then the second cylindrical container can be removed from the first cylindrical container. The filter that has been captured can be easily taken out.
【００３５】検体中の塵埃を除去するための第２のフィ
ルタが第１の筒状容器に取り付けられている場合には、
液体の検体中の塵埃や汚染成分を除去することができ、
より高精度に細菌検査を行うことができる。When the second filter for removing dust in the sample is attached to the first cylindrical container,
Dust and contaminants in liquid samples can be removed,
Bacteria test can be performed with higher accuracy.
【図１】本発明の一実施形態に係る細菌検査用サンプル
収集装置の縦断面図。FIG. 1 is a vertical cross-sectional view of a sample collecting apparatus for bacterial test according to an embodiment of the present invention.
【図２】本発明の一実施形態に係る細菌検査用サンプル
収集装置の分解状態を示す縦断面図。FIG. 2 is a vertical cross-sectional view showing a disassembled state of the sample collecting apparatus for bacterial test according to the embodiment of the present invention.
【図３】（ａ）〜（ｃ）は、本発明の一実施形態で用い
られる第１の筒状容器を構成する筒状部材の平面図、縦
断面図及び底面図。3A to 3C are a plan view, a vertical cross-sectional view, and a bottom view of a tubular member that constitutes a first tubular container used in an embodiment of the present invention.
【図４】（ａ）及び（ｂ）は、本発明の一実施形態で用
いられる第１の筒状容器を構成するための筒状部材を示
す平面図及び縦断面図。4 (a) and 4 (b) are a plan view and a vertical cross-sectional view showing a tubular member for forming a first tubular container used in an embodiment of the present invention.
【図５】（ａ）〜（ｃ）は、本発明の一実施形態で用い
られる第２の筒状容器の平面図、側面図及び底面図を示
す。5A to 5C are a plan view, a side view, and a bottom view of a second cylindrical container used in the embodiment of the present invention.
【図６】図５に示した第２の筒状容器に用いられた突起
及び係止部を説明するための拡大正面図。FIG. 6 is an enlarged front view for explaining a protrusion and a locking portion used in the second tubular container shown in FIG.
【図７】（ａ）及び（ｂ）は、本発明の一実施形態で用
いられる第２の筒状容器の平面図及び縦断面図。7A and 7B are a plan view and a vertical sectional view of a second cylindrical container used in an embodiment of the present invention.
【図８】（ａ）及び（ｂ）は、本発明の一実施形態で用
いられる第３の筒状容器の平面図及び縦断面図。8A and 8B are a plan view and a vertical sectional view of a third cylindrical container used in an embodiment of the present invention.
１…細菌検査用サンプル収集装置 ２…ハウジング ３…第１の筒状容器 ４…第２の筒状容器 ５…第３の筒状容器 ５ｃ…中空針 ６，７…筒状部材 ６ａ…開口 ８…第２のフィルタ ９…フィルタ取付部材 １０…フィルタ １１…液体収納容器 １２…容器本体 １３…栓体 Ａ…排出空間 1. Sample collection device for bacteria test 2 ... Housing 3 ... the first cylindrical container 4 ... Second cylindrical container 5 ... Third cylindrical container 5c ... Hollow needle 6, 7 ... Cylindrical member 6a ... opening 8 ... Second filter 9 ... Filter mounting member 10 ... Filter 11 ... Liquid storage container 12 ... Container body 13 ... Plug A ... Discharge space
───────────────────────────────────────────────────── フロントページの続き (72)発明者 横井 正之 大阪市北区西天満２−４−４ 積水化学工 業株式会社内 (72)発明者 澤田 芳秀 大阪市北区西天満２−４−４ 積水化学工 業株式会社内 Ｆターム(参考） 4B029 AA08 AA09 BB02 CC01 GB01 GB02 HA06 HA10 ─────────────────────────────────────────────────── ─── Continued front page (72) Inventor Masayuki Yokoi 2-4-4 Nishitenma, Kita-ku, Osaka Sekisui Chemical Co., Ltd. Business (72) Inventor Yoshihide Sawada 2-4-4 Nishitenma, Kita-ku, Osaka Sekisui Chemical Co., Ltd. Business F-term (reference) 4B029 AA08 AA09 BB02 CC01 GB01 GB02 HA06 HA10
Claims (3)
ルを収集するための細菌検査用サンプル収集装置であっ
て、 検体が注がれる開口と、前記開口から注がれた検体を濾
過するとともに、検体に含まれていた細菌を捕捉するた
めのフィルタが取り付けられるフィルタ取付部と、前記
フィルタで濾過された検体が排出される排出空間とを有
し、前記排出空間に連通されておりかつ前記フィルタか
ら遠ざかる方向に先端が延ばされた中空針とを備えるハ
ウジングと、前記ハウジングのフィルタ取付部に取り付
けられたフィルタとを有する細菌収集器具と、 内部が減圧されておりかつ前記中空針により穿刺され得
る栓体により密封されている液体収納容器とを備えるこ
とを特徴とする細菌検査用サンプル収集装置。1. A sample collecting device for a bacteria test for collecting a sample in a bacteria test using a liquid as a sample, comprising: an opening into which a sample is poured; and a sample poured from the opening, and A filter mounting portion to which a filter for capturing bacteria contained in the sample is mounted, and a discharge space for discharging the sample filtered by the filter, the filter being in communication with the discharge space and the filter A bacteria collecting instrument having a housing having a hollow needle whose tip extends in a direction away from the housing, and a filter attached to a filter mounting portion of the housing; A sample collection device for a bacteria test, comprising: a liquid container that is sealed by the obtained stopper.
ている略筒状の第１の筒状容器と、 前記第１の筒状容器の下方部分に嵌め込まれるように構
成されており、かつ上面に前記フィルタ取付部が設けら
れた略筒状の第２の筒状容器と、 前記第２の筒状容器の下方から第２の筒状容器に嵌め込
まれて、第２の筒状容器とともに前記排出空間を構成し
ており、かつ該排出空間に連通しており、かつ先端が下
方に延ばされた前記中空針が設けられた第３の筒状容器
とを備える、請求項１に記載の細菌検査用サンプル収集
装置。2. A substantially tubular first tubular container having an opening at the upper end for pouring a sample and an open lower end, and a lower portion of the first tubular container. A second tubular container having a substantially tubular shape and having an upper surface provided with the filter mounting portion, and a second tubular container from below the second tubular container. A third cylinder that is fitted and constitutes the discharge space together with the second cylindrical container, is in communication with the discharge space, and is provided with the hollow needle whose tip extends downward. The sample collecting apparatus for a bacteria test according to claim 1, further comprising a container.
検体中の塵埃を除去するための第２のフィルタが取り付
けられている、請求項２に記載の細菌検査用サンプル収
集装置。3. An intermediate height position of the first cylindrical container,
The sample collecting device for a bacteria test according to claim 2, wherein a second filter for removing dust in the sample is attached.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002146443A JP2003038162A (en) | 2001-05-22 | 2002-05-21 | Sampler for testing bacteria |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001152698 | 2001-05-22 | ||
| JP2001-152698 | 2001-05-22 | ||
| JP2002146443A JP2003038162A (en) | 2001-05-22 | 2002-05-21 | Sampler for testing bacteria |
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| Publication Number | Publication Date |
|---|---|
| JP2003038162A true JP2003038162A (en) | 2003-02-12 |
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ID=26615519
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002146443A Pending JP2003038162A (en) | 2001-05-22 | 2002-05-21 | Sampler for testing bacteria |
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| US11921018B2 (en) | 2021-01-06 | 2024-03-05 | Hero Scientific Ltd. | Filtration sampling devices |
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