JP2003033160A - Nutritional composition for prophylaxis or repair promotion of injury in small intestinal tract, nutritional composition for infant and prophylaxis or healing promoter of disorder in small intestinal tract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a nutritional composition usable in prophylaxis or healing promotion of disorder in the small intestinal tract and a prophylaxis or a healing promoter of the disorder in the small intestinal tract. SOLUTION: This nutritional composition for the prophylaxis or healing promotion of the disorder in the small intestinal tract is formulated with α- lactalbumin or an enzymic digest of the α-lactalbumin in an amount of >=1 mass% of the α-lactalbumin.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a nutritional composition for preventing or repairing small intestinal tract injury, a nutritional composition, and an agent for preventing or repairing small intestinal tract injury.

[0002]

2. Description of the Related Art In Japan, it has been a long time since a hygienic environment has been established, and the frequency of diseases such as diarrhea associated with injury of the small intestine of infants has greatly decreased. However, even if only diarrhea due to viral infection such as rotavirus infection in winter is taken up, these diseases are not rare, and there are still times when these diarrhea in infants can be seen. Widely recognized.

On the other hand, not only in the case of such diarrhea caused by viral infection of infants, but also in adults, many diseases in which the microvilli of the small intestinal mucosa are extensively invaded, that is, the so-called small intestinal tract injury is known. There is. In the case of small intestinal injury, the absorption area of the mucosal surface of the small intestine is reduced, or the absorption of nutrients is reduced due to digestive and absorption disorders due to the lack of digestive enzymes. This will lead to a decrease in nutritional status. Therefore, as described above, promptly repair the damaged state of the small intestine,
Improving gastrointestinal function and restoring nutritional status to good condition is very important.

In the treatment of diseases associated with damage to the small intestinal tract, for example, in the case of acute diarrhea caused by viral infection in infants, disaccharide-degrading enzymes such as lactose are deficient due to damage to the mucosal surface of the small intestine. Since the activity is drastically reduced, so-called lactose-free milk in which lactose, which is the main sugar in child-rearing milk, is replaced with sucrose or dextrin,
Soybean milk made from soybean that does not contain lactose at all has been used.

However, these milks for diarrhea, which do not contain lactose at all, can be used in patients with digestive and absorptive ability due to damage to the small intestinal tract to remove abnormal lactose, thereby causing abnormal fermentation and the like. While preventing the spread of diarrhea caused by sucrose, sucrose, dextrin, etc., which have a relatively high digestive and absorption capacity, are used as alternative sugars to replenish the amount of heat and nutrients such as proteins and vitamins and minerals. Is intended. Therefore, little attention was paid to the repair of damage to the small intestine except for the supply of nutrients.

Therefore, there is a problem that the healing ability of each patient largely depends on the recovery of the symptoms such as diarrhea. For example, in the case of a patient who was not always in good nutrition until then, there was a problem that the period required for recovery of symptoms was long. Therefore, there has been a demand for a nutritional composition that can be used for prevention or promotion of repair of small intestinal tract injury and an effective agent for preventing or repairing small intestinal tract injury.

[0007] By the way, α-lactalbumin is a major protein contained in milk or human milk, and is known to be involved in lactose biosynthesis in the mammary gland as a lactose synthase together with galactosyltransferase. ,
It is believed that this is related to the fact that lactose accounts for the majority of sugars in milk or human milk. In addition, α-lactalbumin is known to have a high ratio of sulfur-containing amino acids and is rich in essential amino acids such as cystine and tryptophan, so it is considered to be a nutritionally important source of amino acids. Has been. Therefore, a baby milk product containing milk protein as a component protein is disclosed in Japanese Patent Application Laid-Open No. H8-78242.
-214775 discloses a baby milk product fortified with an α-lactalbumin component to make the total amino acid composition similar to human milk, and the protein disclosed in Japanese Patent No. 2622789 which is not present in breast milk. Therefore, various proposals have been made, such as a breast milk substitute containing a fraction having a high α-lactalbumin content while reducing β-lactoglobulin that acts as an allergen for infant allergies.

However, the inventions described in these prior art documents are all aimed at approximating the amino acid composition and protein composition of a dairy product for breastfeeding or a milk substitute as breast milk. Therefore, these prior art documents neither describe nor suggest prevention or promotion of repair of small intestinal tract injury.

[0009] Further, Japanese Patent Publication No. 5-508542 discloses a milk product extract composition obtained from a milk product, and a pharmaceutical or veterinary composition for treating gastrointestinal damage, diseases or ulcers using the milk product extract composition. The thing is disclosed. It is also described that casein, α-lactalbumin and the like may be contained in an amount of less than 0.5% by weight based on the total weight of the milk product extract composition. However, the milk product extract composition is produced from, for example, cheese whey, which is obtained by reducing or removing its salt and / or main protein components, and protein is actively removed. . As described above, the protein is actively removed, and the content of casein, α-lactalbumin, etc. is regulated to a small amount of less than 0.5% by weight. In the invention described in this prior art document, it is understood that lactalbumin is preferably not blended as much as possible. It should be noted that, of course, the prior art document does not describe the effect of α-lactalbumin for preventing or repairing small intestinal tract injury. Further, JP-A-8-301776 describes a substance for promoting differentiation of small intestinal epithelium using a non-protein nitrogen fraction in milk. In the fraction, the protein α
-Lactalbumin etc. are actively excluded, and α-lactalbumin is not contained in the relevant fraction. As a matter of course, this prior art document does not describe any effect of α-lactalbumin for preventing or repairing small intestinal injury. As described above, the effect of preventing or repairing damage to the small intestinal tract of α-lactalbumin has not been known at all.

The present invention has been made in view of the above circumstances, and provides a nutritional composition that can be used for prevention or promotion of repair of small intestinal tract injury and an agent for preventing or repairing small intestinal tract injury. It is an issue.

[0011]

In view of the situation as described above, the present inventors have conducted research and examination in a wide range of fields, focusing on the point of repairing injury of the small intestine. As a result, for example, when the infant whose intestinal tract was injured by diarrhea or the like was an artificial nutrition infant, the so-called lactose-free milk etc., which was replaced with sucrose or dextrin by reducing or removing the lactose content, was used. I needed to feed her. On the other hand, if the infant with an injury to the small intestinal tract was a breast-fed infant or a mixed-fed infant, it often continued to feed the breast milk, in which case the breast milk contained a large amount of lactose. Therefore, we came to notice that there is no adverse effect that the period required for healing is extended. Furthermore, the present inventors have said that, in addition to the effect of removing the cause of infection by the protective factor for infection such as lactoferrin contained in breast milk, there is an ingredient effective in preventing or repairing intestinal injury in breast milk. As a result of intensive studies under the assumption, cell growth factors such as EGF, which have been known to exist, and the above-mentioned milk product extract composition (Table 5
No. 508542), a substance promoting differentiation of small intestinal epithelium due to non-protein nitrogen fraction in milk (JP-A-8-30177).
No. 6), etc., but α-lactalbumin, which is a major protein contained in breast milk, has an effect of preventing or repairing small intestinal tract injury. It has been found that an effect of preventing or repairing intestinal injury can be obtained. In addition, the effect of preventing or repairing small intestinal tract injury of α-lactalbumin obtained by using a certain amount or more is also effective for preventing or repairing small intestinal tract injury caused by causes other than diarrhea. Heading, completed the present invention. That is, the nutritional composition for preventing or repairing small intestinal injury of the present invention comprises α
-The enzyme digested product of lactalbumin and / or α-lactalbumin is blended in an amount of 1% by mass or more in terms of α-lactalbumin. The nutritional composition for preventing or repairing damage to the small intestinal tract can also be used for lactose intolerant patients who cause damage to the small intestine due to lactose. The small intestinal tract injury may be diarrhea. Furthermore, the lactose content per 100 g of solid content of the nutritional composition for preventing or repairing small intestinal tract injury is preferably 1 g or less. In addition, the nutritional composition for infants of the present invention is a nutritional composition containing 1% by mass or more of α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin in terms of α-lactalbumin. The composition is characterized in that the lactose content per 100 g of solid content is 1 g or less. Further, the preventive or repair promoting agent for small intestinal tract injury of the present invention is characterized by containing 1% by mass or more of α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin in terms of α-lactalbumin. . This agent for preventing or repairing intestinal injury is preferably used for lactose intolerant persons. The small intestinal tract injury may be diarrhea.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION (Nutrient composition for preventing or repairing small intestinal tract injury) First, the nutritional composition for preventing or repairing small intestinal tract injury of the present invention will be described. In the nutritional composition, one or a mixture of α-lactalbumin and an enzymatic digest of α-lactalbumin is used. α-lactalbumin is not particularly limited. For example, it may be derived from human milk as well as milk. Further, purified α-lactalbumin may be used, or it may be blended as a whey protein containing α-lactalbumin. More specifically, for example, α-lactalbumin is produced by an ammonium sulfate precipitation method according to a conventional method (for example, Kinjiro Yukawa, “The latest revised dairy technology handbook”, Japan Dairy Technology Association, 120-122, 1975). can do. More conveniently, for example, commercially available α-lactalbumin (for example, manufactured by Davisco Foods, Inc.) can be used.

Also, an enzymatic digestion product of α-lactalbumin can be used. The enzyme digest is a raw material α-lactalbumin or whey protein containing it,
It is obtained by digesting (decomposing) this with an enzyme such as a protease. Examples of the raw material used at this time include α-lactalbumin derived from animal milk of cattle, humans, etc. or whey protein containing the same. Further, a protein concentrate obtained by subjecting these α-lactalbumin or a whey protein containing it to a concentration treatment can also be used. For the concentration treatment, a method such as ultrafiltration or ion exchange resin can be applied. Further, these raw materials may be used alone or in combination of two or more.

Examples of the enzyme include animal origin (eg trypsin, chymotrypsin, pepsin, pancreatin etc.), plant origin (eg papain etc.), or microbial origin (eg lactic acid bacteria, yeast, mold, Bacillus subtilis, actinomycetes). etc)
The protease or peptidase of the above can be exemplified. These can be used alone or in appropriate combination.

In the nutritional composition for preventing or repairing small intestinal injury, α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin is added to α-lactalbumin in the nutritional composition.
1 mass% or more in terms of lactalbumin, preferably 3.
It is mixed in a proportion of 5% by mass or more and substantially 33% by mass or less. If it is less than 1% by mass, a sufficient effect cannot be obtained. In the enzymatic digestion product of α-lactalbumin, α
-Because lactalbumin is contained in a decomposed state, in the nutritional composition of the present invention, the blending amount of α-lactalbumin and / or the enzymatic digestion product of α-lactalbumin is the same as that of α-lactalbumin before decomposition. Based on quantity.

The nutritional composition for preventing or repairing small intestinal tract injury uses the same materials as conventional ones except that α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin is added at the above concentration. , Can be manufactured similarly. Specific examples of the nutritional composition for preventing or repairing small intestinal injury include nutritional compositions for infants and adults, and nutritional compositions for adults. The nutritional composition for infants includes, for example, modified milk (for example, infant formula, low birth weight formula, follow-up milk, and formula for allergic diseases, lactose-free milk, inborn errors of metabolism). Special milk, etc.) and the like. Here, the modified milk is a food produced mainly by adding milk or dairy products with nutrients necessary for infants, and in the infancy, it is mainly used as a food substitute for breast milk. ,
It is used as a food that plays a complementary role in breast milk replacement or nutritional intake in infancy, and is manufactured for the purpose of contributing to nutritional intake suitable for infants with specific congenital or acquired diseases. Food. Examples of adult nutritional compositions include enteral nutritional supplements and concentrated liquid foods.

Examples of materials to be blended include proteins other than α-lactalbumin, lipids, sugars, vitamins, minerals and the like. Note that the exemplified nutritional composition generally contains these materials as main components. Other compounds that can be blended include, for example, functional proteins (lactoferrin, lactoperoxidase, etc.),
Examples include nucleic acids, dietary fiber, oligosaccharides (lactulose, raffinose, etc.), useful microorganisms (lactic acid bacteria, bifidobacteria, etc.), and the like.

The form of the nutritional composition for preventing or repairing intestinal injury is not particularly limited, and various forms such as liquid form, powder form and tablets can be applied. In addition, it can be ingested by the same ingestion method as the conventional one.

Since the nutritional composition for preventing or repairing small intestinal tract injury of the present invention is effective for the small intestinal tract injuries of lactose intolerant patients who cause small intestinal tract injuries caused by lactose, its prevention or repair accelerating. It is preferable to use it for. Further, since the small intestinal tract injury is effective in the case of diarrhea, it is preferably used for prevention or promotion of repair. Further, the nutritional composition for preventing or repairing small intestinal injury of the present invention, in addition to small intestinal injury caused by diseases such as diarrhea, so-called through the small intestine for digestive disorders and surgery. Since it also has the effect of promoting the repair of damage due to atrophy of the small intestinal mucosa caused by interruption of enteral nutrition, etc., it can also be used for its prevention or repair promotion.

In the nutritional composition for preventing or repairing intestinal injury, the solid content of the nutritional composition is 10%.
Lactose content per 0 g is 1 g or less, preferably 50 mg
The following is preferable. In this case, the lower the lactose content, the more preferable, so the lower limit value is not particularly limited. By reducing lactose in this way, it is extremely effective in preventing or repairing small intestinal injury in lactose intolerant individuals who cause small intestinal injury due to lactose. In addition, as in the case of acute diarrhea caused by viral infection of infants described above, for infants or adults with small intestinal injury in which the activity of a disaccharide-degrading enzyme such as lactose is rapidly reduced due to injury of the small intestinal mucosal surface. Is also effective. To achieve the lactose compounding conditions, the compounding composition of the material may be set so as to satisfy the conditions. For example, in a product containing a large amount of lactose such as a modified milk, lactose is reduced to 1 g or less by substituting a part or all of lactose with sucrose or dextrin so as to reduce or not contain lactose. can do. Also, soybean milk made from soybean can be used. In particular, in a product containing a large amount of lactose such as a modified milk, the recovery of the small intestinal tract injury tends to be delayed due to the presence of lactose. Therefore, it is preferable to apply such a configuration because the effect is large.

(Nutritional composition for infants) In the present invention, 1% by mass or more of α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin in terms of α-lactalbumin is added, and Also proposed is a nutritional composition for infants having a lactose content of 1 g or less per 100 g of solid content. The use of this nutritional composition is not limited except for infants and infants, and it can also be used for applications other than prevention of small intestinal injury or promotion of repair. The composition of the nutritional composition for infants is the same as the nutritional composition for preventing or repairing small intestinal tract injury described above except that the use is not limited. In addition, in particular, a modified milk, which is a typical example of a nutritional composition for infants, generally contains lactose in many cases. Therefore, the solid content of this prepared milk is 10
It is preferable that the lactose content per 0 g is 1 g or less, since the effect of preventing or repairing small intestinal tract injury can be further enhanced in infants and the like who have small intestinal tract injury due to lactose.

(Prevention or Repair Promoter of Small Intestinal Injury) The preventive or repair promoter of small intestinal injury according to the present invention comprises α-lactalbumin and / or an enzymatic digest of α-lactalbumin in terms of α-lactalbumin. It is characterized in that it is blended in an amount of 1% by mass or more, preferably 10% by mass or more. The daily intake of the agent for preventing or repairing small intestinal tract injury is preferably 0.2 g or more in terms of α-lactalbumin per kg body weight for infants. In the case of an adult , the daily intake of the preventive or repair promoting agent for small intestinal injury is 0.
The amount is preferably 1 g or more, and it is preferable to ingest such an amount of the agent for preventing or repairing small intestinal tract injury once a day or divided into several times a day.

Since the preventive or repair promoting agent for small intestinal tract injury is effective for the small intestinal tract injury for lactose intolerant who causes small intestinal tract injury due to lactose, it is used as a preventive or repair accelerating agent. It is preferable. In addition, since the small intestinal tract injury is effective in the case of diarrhea, it is preferable to use it as a preventive or repair accelerating agent. In addition to small bowel injuries caused by diseases such as diarrhea, injuries due to atrophy of the small intestinal mucosa caused by interruption of so-called enteral nutrition via the small intestinal tract due to digestive diseases and surgery. Since it also has the effect of promoting the repair of the, it can be used as a preventive or repair promoting agent. Further, the use of the agent for preventing or repairing intestinal injury is not particularly limited. For example, it can be added to a nutritional composition such as the above-mentioned modified milk, or can be applied to a drug or the like. That is, it can be used as an additive or a preparation. In that case, the blending amount is preferably adjusted appropriately so as to satisfy the above conditions.

The nutritional composition for preventing or repairing the small intestinal tract according to the present invention is an active ingredient for repairing the small intestinal tract injury caused by diarrhea caused by lactose, or the small intestinal tract injury caused by viral or bacterial infection. It was completed by the finding that certain α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin has the effect of promoting it, and its mechanism of action is presumed as follows. That is, Emmanuel Leventhal, Textbook of Gastroenterology and Nutrition in Infancy, Second Edition, pages 459, 464, 11
75 pages, Raven Press, 1989 (Emanuel Lebenthal
ed .: Textbook of Gastroenterology and Nutrition
in Infancy Second Edition p.459, p.464, p.1175, Rav
As described in "En Press, 1989), in the case of diarrhea caused by lactose or diarrhea caused by viral or bacterial infection, the epithelium (brush border membrane) of the small intestinal tract is damaged. However, this results in the loss of disaccharide-degrading enzymes such as lactose-degrading enzyme (lactase) localized in the small intestinal epithelium. In particular, lactose-degrading enzyme (lactase) is the enzyme most susceptible to small intestinal injury,
It is also known to be the enzyme that recovers to the normal state with the longest delay. And V. S. As described in Buckard, “Breast milk and infant formula,” p. 164, Dobunshoin, 1987, secondary lactose-degrading enzymes (lactases), etc. A deficiency causes indigestion and absorption of disaccharides such as lactose, and unabsorbed saccharides such as lactose migrate from the lower small intestine to the large intestine as they are, increasing the osmotic pressure in the intestinal tract and drawing water from surrounding tissues. Or, by causing excessive fermentation by intestinal bacteria present there, and by producing a large amount of gas such as organic acids and carbon dioxide due to overfermentation, the spread of diarrhea and abdominal pain,
It causes symptoms such as bloating. Therefore, lactose-degrading enzyme (lactase) is caused by such small intestinal injury.
When the lactose is lost, the intestinal epithelium (brush border membrane) is allowed to heal spontaneously while limiting the intake of lactose. However, the enzymatic digestion product of α-lactalbumin and α- or lactalbumin mixed in the nutritional composition and the like according to the present invention repairs such damage of the small intestine, that is, small intestinal epithelium (brush border membrane). Defective by promoting the recovery of the small intestinal epithelium to the normal state by the action of promoting the differentiation and proliferation of the constituent cells, and accelerating the expression of disaccharide-degrading enzymes such as lactose-degrading enzyme (lactase) present there. By recovering from the condition and promoting normal digestion and absorption of lactose and the like, recovery of various symptoms due to diarrhea and the like is promoted faster. As described above, the recovery effect of lactose-degrading enzyme (lactase) activity correlates with the repair effect of small intestinal injury. In addition, such a differentiation-promoting action of small intestinal epithelium by α-lactalbumin suppresses the progress of slight inflammation that causes diarrhea if it deteriorates, and manifests with symptoms such as diarrhea. Since it suppresses the development of small intestinal injury, it is clear that it also exerts a preventive effect against diarrhea and the like. As described above, by using at least one of the nutritional composition for preventing or repairing small intestinal tract injury, the nutritional composition for infants, or the agent for preventing or repairing small intestinal tract injury of the present invention, A preventive or repair promoting effect is obtained. For example,
Prevention of diseases associated with damage to the small intestinal tract of infants or adults, or by the effect of repairing, it is possible to prevent or quickly improve the digestive and absorption damage of nutrients due to the disease,
The adverse effects on nutritional status or development can be minimized. In addition, the recovery time is shortened when the small intestine is injured.

[0025]

EXAMPLES The present invention will be described in detail below with reference to examples. (Example 1) 22 kg of α-lactalbumin (manufactured by Davisco Foods) and 12 kg of lactate casein (manufactured by New Zealand Daily Board) were dissolved in 280 kg of purified water,
1 kg of a mineral mixture (prepared by Tomita Pharmaceutical Co., Ltd.) was dissolved in 10 kg of purified water and added. Subsequently, this solution was heated to 60 ° C., 50 kg of adjusted fat (manufactured by Taiyo Oil & Fats Co., Ltd.), 100 kg of malt dextrin (manufactured by Matsutani Chemical Co., Ltd.), 11 kg of sucrose (manufactured by Nippon Sugar Beet Sugar Co., Ltd.), and a vitamin mixture (Tanabe Seiyaku Co., Ltd.). (Manufactured by the company) 40 g, homogenized, 120 ℃
It was sterilized for 2 seconds, concentrated, and spray-dried to obtain about 190 kg of lactose-free milk powder for lactose intolerant.

Example 2 In 7 kg of warm water (60 ° C.),
Add 5 kg of α-lactalbumin (Davisco Foods) and 15 kg of dextrin (Sanmatsu Kogyo),
A K-homomixer (manufactured by Tokushu Kika Kogyo Co., Ltd.) was used for dissolution and dispersion to prepare a liquid material. 140 g of succinic acid monoglyceride (manufactured by Kao), 2.2 g of adjusted fat (manufactured by Taiyo Oil & Fat Co., Ltd.), and mineral mixture (manufactured by Tomita Pharmaceutical Co., Ltd.)
400 g and 20 g of vitamin mixture (manufactured by Tanabe Seiyaku Co., Ltd.)
Was added and pre-emulsified using a TK homomixer (made by Tokushu Kika Kogyo Co., Ltd.), and water was added to adjust the total amount to 100 kg. Next, the pre-emulsion was homogenized by using a high-pressure homogenizer (manton Gorin Co., Ltd.) to homogenize the two-step treatment of 5 MPa in the first step and 50 MPa in the second step five times to prepare a liquid liquid food of about 92 kg. Liquid liquid food about 11kg
200 ml each was filled in a retort pouch (manufactured by Toyo Seikan Co., Ltd.), and then sterilized by a retort sterilizer (manufactured by Hisaka Seisakusho) at 125 ° C. for 10 minutes to prepare 50 liquid liquid foods.

Example 3 5.5 kg of α-lactalbumin (manufactured by Davisco Foods) and 10 kg of casein (manufactured by Nippon Protein Co., Ltd.) were dissolved in 190 kg of purified water, and 550 g of a mineral mixture (prepared by Tomita Pharmaceutical Co., Ltd.) was purified to 10 kg. It was dissolved in water and added. Subsequently, the solution was heated to 60 ° C., 20 kg of adjusted fat (manufactured by Taiyo Oil & Fat Co., Ltd.), 49 kg of dextrin (manufactured by Showa Sangyo Co., Ltd.), 5 kg of sucrose (manufactured by Nippon Beet Sugar Co., Ltd.), 10 kg of lactose (manufactured by Mirai Co., Ltd.), And 23 g of vitamin mixture (manufactured by Tanabe Seiyaku Co., Ltd.) and homogenized,
It was sterilized at 120 ° C. for 2 seconds, concentrated and spray-dried to obtain about 97 kg of infant formula. The infant fed with the infant formula of Example 3 also showed good growth as the diarrhea symptom recovered.

Example 4 The results of feeding various diets to rats were compared as follows. 1) Preparation of feed A control feed containing casein as a protein source in the formulation shown in Table 1, a lactose-containing feed in which most of sugars in the control feed were replaced with lactose, and a part of casein in the control feed was α-. A feed containing α-lactalbumin substituted with lactalbumin was prepared.

[0029]

[Table 1]

2) Test method Fifteen 3-week-old male SD rats were acclimated and fed with a control feed for 1 week, and then fed with a lactose-containing feed for 7 days to experimentally induce diarrhea. After that, the mice were divided into 3 groups so that the average body weight was the same. One group among them was fed with α-lactalbumin-containing feed for 3 days (hereinafter referred to as LA group), the other group was fed with control feed for 3 days (hereinafter referred to as LC group), and the remaining The lactose-containing feed was continuously fed to the 1st group for 3 days (hereinafter referred to as the LL group).

3) Test Results-Observation of Diarrhea Symptoms All of the 3 groups that were fed the lactose-containing feed for 8 days from the start of the test caused diarrhea symptoms by ingesting an excessive amount of lactose. L fed the control feed for 3 days thereafter
The diarrhea symptom gradually recovered in both the C group and the LA group fed the α-lactalbumin-containing feed, but the diarrhea symptom continued in the LL group fed the lactose-containing feed. -Activity of lactose-degrading enzyme After completion of the test for all 17 days, rats in each group were dissected, duodenum, jejunum, and ileum were collected in accordance with a standard method, mucous membranes were scraped from each site, and protein content was determined in a standard method. The activity of lactose-degrading enzyme (lactase), which is a small intestinal mucosal enzyme, was measured. The results are shown in Table 2. The significance level was compared between the LC group and the LA group. In addition, N. S. Is evaluated as no significant difference, and P <0.05 is evaluated as significant difference. Therefore, it was confirmed that in the LA group, the effect of recovering the activity of the lactose-degrading enzyme was greater than that in the LC group. This means that the degree of recovery of the lactose-degrading enzyme lost from the epithelium of the small intestine damaged by diarrhea caused by lactose
Compared to the LC group fed a control diet without lactose,
Α-containing lactose without addition of α-lactalbumin
This shows that the LA group fed with the lactalbumin-containing feed was statistically significantly promoted.
Therefore, as described above, the recovery effect of lactose-degrading enzyme, which is known to be most slowly recovered in the repair of small intestinal injury, was shown to have a promoting effect. It is nothing but confirmation of the effect of promoting repair of small intestinal injury.

[0032]

[Table 2]

From the results of these Examples and Comparative Examples, α-
It was confirmed that lactalbumin has a preventive or repair promoting effect on small intestinal injury represented by diarrhea.

[0034]

As described above, in the present invention,
It is possible to provide a nutritional composition and an agent for preventing or repairing small intestinal tract injury, which is excellent in the effect of preventing or repairing small intestinal tract injury.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Takashi Shimizu             Morinaga 5-83 Higashihara 5-chome, Zama City, Kanagawa Prefecture             Dairy Co., Ltd. Nutritional Science Research Institute (72) Inventor Tomomi Kawakami             Morinaga 5-83 Higashihara 5-chome, Zama City, Kanagawa Prefecture             Dairy Co., Ltd. Nutritional Science Research Institute (72) Inventor Reiko Koizumi             Morinaga 5-83 Higashihara 5-chome, Zama City, Kanagawa Prefecture             Dairy Co., Ltd. Nutritional Science Research Institute F-term (reference) 4B018 MD20 MD22 MD71 ME11                 4C084 AA02 BA43 BA44 CA18 CA20                       CA38 CA59 DA36 NA14 ZA66                       ZA72 ZC21

Claims (8)

[Claims] 1. A nutritional composition for preventing or repairing small intestinal tract injury, which comprises 1% by mass or more of α-lactalbumin and / or an enzymatic digest of α-lactalbumin in terms of α-lactalbumin. . 2. The nutritional composition for preventing or repairing small intestinal injury according to claim 1, which is for lactose intolerant who causes small intestinal injury due to lactose. 3. The nutritional composition for preventing or repairing small intestinal injury according to claim 1, wherein the small intestinal injury is diarrhea. 4. The nutritional composition for preventing or repairing small intestinal injury according to claim 1, wherein the nutritional composition has a lactose content of 1 g per 100 g of solid content.
The following nutritional composition for preventing or promoting repair of intestinal injury. 5. A nutritional composition containing 1% by mass or more of α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin in terms of α-lactalbumin, which is based on 100 g of solid content of the nutritional composition. A nutritional composition for infants, which has a lactose content of 1 g or less. 6. A preventive or repair accelerating agent for small intestinal injury, which comprises 1% by mass or more of α-lactalbumin and / or an enzymatic digestion product of α-lactalbumin in terms of α-lactalbumin. 7. The agent for preventing or repairing small intestinal tract injury according to claim 6, which is for lactose intolerant persons. 8. The preventive or repair-promoting agent for small intestinal injury according to claim 6 or 7, wherein the small intestinal injury is diarrhea.