JP2003009866A - Method for discriminating animal species by sine method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for discriminating animal species by SINE(short interspersed element) method. SOLUTION: This method comprises the steps of preparing each genomic DNA library from at least one animal species to be discriminated, isolating each clone including an orthologous gene locus specifically inserted with a SINE family or subfamily in at least one of the animal species, amplifying by PCR the sequence of the gene locus for each of the animal species using a set of PCR primers annealing on the flanking sequences located on both ends of the SINE family or subfamily in the gene locus, and discriminating the animal species based on the presence/absence of a band indicating the presence of the gene locus by making a gel electrophoresis of the resultant PCR product.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for discriminating animal species by the SINE method. More particularly, SINE
The present invention relates to a method for identifying species of mammals including whale eyes using subfamilies.

[0002]

BACKGROUND OF THE INVENTION Automation of DNA sequencing and molecular cloning techniques has dramatically expanded access to the genome of organisms. In addition, the flood of genomic digital information
He attracts many biologists to computer-based bioinformatics. The results of comprehensive efforts such as the Human Genome Project have shown in great detail what was revealed about 30 years ago by nucleic acid regeneration studies. That is, the vast majority of eukaryotic genomes are composed of repetitive elements that do not code for specific protein products and have no apparent function (Kazazian et al.,
1998). As a result of genome analysis, so-called "junk yard", which does not encode proteins, has newly become a "jungle" of dynamic molecules filled with active elements such as DNA type transposable elements and RNA transposable elements. Is recognized. Retroposons are an interesting ubiquitous repeat sequence that is believed to be available as a taxonomic tool for phylogenetic reconstruction and population analysis, and are members of the RNA-based transposable element. (Brosius,
1991; Shedlock and Okada, 2000). Therefore, retroposon diagnosis provides an important bridge between relevant subfields of evolutionary biology at the individual or species level.

The term "retroposition" refers to how the above elements move between the parent locus and the target locus in the genome by RNA intermediates (Rogers, 19
83). This copy-and-paste process creates a reverse flow of genetic information from RNA to chromosomal DNA (Weine
r, et al., 1986, Schmid, 1996). It differs from DNA-type transposons, such as the mariner and P element, which jump around the chromosomes in a manner that leaves no original copy at the parental locus, i.e. cut and paste (Clark and Kidwell, 1997; Hartl et a
l, 1997). An important feature used to classify retroposons is whether they encode reverse transcriptase (RTase), an enzyme essential for self-amplification.
(Okada, 1991; Eickbush, 1994). Among retroposons that do not self-amplify, short interspe
Since many rsed elements (SINEs) exist in the genome, they are considered to be extremely useful for the study of taxonomy of eukaryotes. SINE is 70-500b in size
It is in the p range and is divided into two categories, those derived from tRNA and those derived from 7SL RNA. 7 SLR
NA-derived SINEs contain only two SINE families, the Alu family of primates and the B1 family of rodents. All other SINEs examined so far have been shown to be from tRNA. LINE encoding RTase for amplification
Also falls into two categories. That is, a mammalian L1 that does not require a specific sequence for amplification but only a simple poly A sequence for reverse transcription, and a stringent 3 ′ end for recognition by RTase for amplification. Are other categories, including most LINEs, that require unique sequence motifs. Most non-mammal SINs
E and LINE share the same 3'terminal tail sequence, the presence of which allows SINE to be amplified by RTase encoded by LINE (Ohshima et al.
al, 1997; Okada et al, 1997; Terai et al., 199
8). In mammals, SINEs, including those derived from tRNA and 7SLRNA, appear to be amplified with the help of RTase encoded by mammalian L1. Therefore, in this case, the conserved sequence motif above is
Not observed in the 3'end tail of SINE. A general diagram for the SINE retro position is shown in FIG.

The fact that retropositions always occur unidirectionally and that more than 10 ⁴ copies of SINE are scattered throughout the host genome make SINE insertion analysis a powerful new approach to molecular taxonomy. It makes things stand out. This SINE insertion analysis shows that other forms of character data, most notably DNA
Complementary sequence and morphology analysis (eg Murata et al,
1993, Shimamura et al., 1997, Takahashi et al, 199
8; Nikaido et al, 1999). S as a taxonomic tool
Papers on INE evolution and their importance are available
(Weiner et al, 1996, Deininger and Batzer, 1993,
Schmid, 1996, Rokas and Holland, 2000; Shedlock an
d Okada, 2000, Shedlock et al. 2000).

By the way, in the discrimination of animal species including whales, after amplifying a specific region of the mitochondrial gene by PCR, they are electrophoresed on an agarose gel to confirm the amplification, and then the sequencing method is used. It has been used to determine the nucleotide sequence, and the difference in the sequence is used as an index for species discrimination. Due to the high cost of the sequence method experimental equipment, most of these analyzes are currently performed by specialized companies. Therefore, in order to discriminate species for a huge number of individuals, the number of analyzes is naturally enormous and the cost thereof is enormous. Furthermore, the analysis of nucleotide sequences still has some inadequacies and uncertainties as described below, and their interpretation is often regarded as a problem.

[0006]

Therefore, there is still a need for an assay method for discriminating animal species including whales simply, quickly and inexpensively without using the analysis of nucleotide sequences.

[0007]

Means for Solving the Problems Now, the present inventor
It has been discovered that INE can be used not only for the study of phylogeny and taxonomy as described above, but also for a simple, rapid, and inexpensive assay method for species identification of animals including whales.
As a result of repeated intensive experiments on a wide range of samples, the certainty was proved and the present invention was completed.
That is, as will be described in detail below, if the SINE insertions are common, it is possible to systematically clarify that some species of those common insertions are monogenic to each other. It can be seen that if the SINE family or subfamily of S. is unique within a particular species, the presence or absence of the insertion can be used for species discrimination. Methods for isolating and identifying SINE subfamily sequences unique to such species are also within the scope of the present invention. Conventional SINE
Although phylogenetic trees have been created using families, it has been done without using the SINE family and classifying them as subfamilies. SINE enables species identification
Can only be performed with SINE that has been recently amplified, so it is necessary to specifically isolate the recently amplified subfamily. This method submits the method.

In one aspect of the present invention, SINE
A method for discriminating an animal species according to the method, comprising the steps of: preparing a genomic DNA library from each of one or more animal species to be discriminated; SIN in seed
A clone containing an orthologous locus in which the E family or subfamily is specifically inserted is isolated respectively; PCR of the sequences of the loci for each of the above animal species using
And the PCR product obtained is subjected to gel electrophoresis, and the species of the animal is discriminated by the presence or absence of a band indicating the presence of the SINE family or subfamily insertion locus. To be done. The SINE family or subfamily has SEQ ID NO: 3
It can be DNA having a sequence selected from the group consisting of ~ 7 or SINE DNA which hybridizes with the above DNA under stringent conditions. For example, the SINE subfamily is a DNA having the CD sequence shown in SEQ ID NO: 6 or the CDO sequence shown in SEQ ID NO: 7.

In another embodiment of the present invention, SINE family or subfamily DNA having a sequence selected from the group consisting of SEQ ID NOS: 3 to 7 or SINE hybridizing with the above DNA under stringent conditions.
DNA is provided. The Ba orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 8 to 21.
Whether it is ndo1, Sp316 corresponding to the sequence shown in any one of SEQ ID NOs: 22 to 35, or SEQ ID NO: 36.
~ Mago19 corresponding to the sequence shown in any one of 46
Or is Ishi14 corresponding to the sequence shown in any one of SEQ ID NOS: 47 to 51, or SEQ ID NOS: 52 to 65
Corresponding to the sequence shown in any one of SEQ ID NOs: 66 to 79, Ishi38 corresponding to the sequence shown in any one of SEQ ID NOS: 66 to 79, or the sequence shown in any one of SEQ ID NOS: 211 to 224. Mago24, Mago26 corresponding to the sequence shown in any one of SEQ ID NOs: 225 to 238, Mago32 corresponding to the sequence shown in any one of SEQ ID NOs: 80 to 93, or SEQ ID NOs: 94 to 98 Which corresponds to the sequence shown in any one of SEQ ID NOs: 99 to 112, the sequence corresponding to the sequence shown in any one of SEQ ID NOS: 99 to 112, or the sequence shown in any one of SEQ ID NOS: 113 to 117. Whether it is Mago21 or Mago22 corresponding to the sequence shown in any one of SEQ ID NOs: 239 to 252,
Sperm8 corresponding to the sequence shown in any one of SEQ ID NOs: 118 to 129, Sperm28 corresponding to the sequence shown in any one of SEQ ID NOs: 130 to 135, or any one of SEQ ID NOs: 136 to 140 Sperm47 corresponding to the sequence shown or SEQ ID NOs: 141 to 1
Amz13 corresponding to the sequence shown in any one of 45, Amz11 corresponding to the sequence shown in any one of SEQ ID NOs: 146 to 151, or SEQ ID NOs: 152 to 15
6 is Tuti24 corresponding to the sequence shown in any one of 6 or Tuti35 corresponding to the sequence shown in any one of SEQ ID NOS: 157 to 171, or SEQ ID NOS: 172 to 1
85 corresponding to the sequence shown in any one of 85, Sp9 corresponding to the sequence shown in any one of SEQ ID NOS: 186-190, or corresponding to the sequence shown in any one of SEQ ID NOS: 191-204 Hump20 to do,
Alternatively, it may be Hump203 corresponding to the sequence shown in any one of SEQ ID NOs: 205 to 210.

In another aspect of the present invention, there is provided a DNA having the sequence shown in any one of SEQ ID NOs: 8 to 238 or an orthologous DNA which hybridizes with the above DNA under stringent conditions. The PCR primer can be selected from the group consisting of SEQ ID NOs: 276 to 325. The animal can be a mammal. Such mammals can belong to the order Artiodactyla.

In another aspect of the invention, SINE
A method of obtaining a consensus sequence of subfamilies, comprising the steps of: sequencing multiple copies of a repeat unit by whole genome transcription in vitro or automatic sequencing of genomic DNA larger than 60 kbp; Alignment to obtain a consensus sequence; find the consensus sequence for the second promoter for RNA polymerase III and create a stem-loop structure containing this sequence; one 5 bases 5'upstream from the stem region As a unit, 3'-PyPyPuPuPu-
Confirm that it is a 5'sequence; consider the next 5 bases forming the anticodon-stem region of the tRNA structure as another unit; the next 7 bases forming the anticodon-loop region of the tRNA structure, Unit, where the 3'end is an AA residue and the 5'end
Confirm that the end is a 3'-TC-5 'residue; consider the next 5 bases as another unit, and confirm the formation of a base pair with the 5 bases assigned to the anticodon-stem region. To place the deletion at the position of the first base 3'of the anticodon-stem in order to align this unit correctly; construct a stem-loop structure for the D region of this tRNA-like structure, Here, the above RNA polymerase II
The presence of two G's in the first promoter region of I was confirmed; the sequence similarity between the above sequence and the tRNA was searched using a DNA database to confirm the similarity of its secondary structure; SI obtained as described above derived from
There is provided the above method, wherein the NE family sequences are aligned to obtain the SINE subfamily from the presence of characteristic nucleotides or deletions; and the SINE subfamily consensus sequence from the SINE subfamily alignment.

The SINE subfamily is the above S
It can be a DNA having a SINE subfamily consensus sequence obtained by the method for obtaining a INE subfamily consensus sequence. In another aspect of the present invention, there is provided a DNA having a SINE subfamily consensus sequence obtained by the method for obtaining a SINE subfamily consensus sequence.

Definitions In the present specification, "SINE" is a short interspersed repetitive sequence having a primary structural length of about 100 to 400 bases, and a tRNA homologous region in order from its 5'end. , T
It refers to those containing an RNA heterologous region and an AT-rich region.
It is characterized by the presence of direct repeats of about 5 to 20 bases, which are considered to be formed when SINE is inserted into the genome, at both ends thereof. In the present specification, "SINE family" means the same tRN
A member of SINE derived from A and having similar sequences to each other. On the other hand, in the present specification, “SINE subfamily” is derived from the same origin (family),
Due to the characteristic mutations, SI seems to be amplified from the same ancestor member in the SINE family.
A member of the NE group. Subfamily is also used interchangeably with type. In the present specification, the orthologous locus means that it is derived from speciation from a common ancestor having two loci. On the other hand, when two genes are generated by gene duplication rather than speciation, they are “paralogous”. It is the orthologous gene, not the paralogous gene, that provides the information necessary for the estimation of the molecular phylogenetic tree.

In the present specification, the "flanking sequence" means a sequence located on both sides of the insertion site when the SINE family or subfamily is specifically inserted into the orthologous locus. PCR primers can be arbitrarily designed within such flanking sequences. In the present specification, the stringent conditions mean template DN.
Put A into a hybrid bag, add an appropriate amount of pre-hybrid solution (6 X SSC, 1% SDS), pack the hybrid bag with a sealer, incubate for 1 hour or more, discard the solution, and remove the hybrid solution (6 X SSC, 1% SDS, 1 X Denh
art's solution, Carrier DNA (Shared Herring Sperm
After adding DNA solution), add a pre-conditioned probe that has been heat-denatured at 95 ° C for 3 minutes, seal the hybrid bag, and then in a 42 ° C water bath overnight (about 15 hours). ) Incubate the membranes with an appropriate volume of wash solution (2X SSC, 1% SD
The conditions under which the probe hybridizes with the template DNA when lightly rinsed with S).

In the present specification, the term "outer group comparison" refers to a species which is assumed to be most closely related to an outgroup, that is, a target organism group, that is, an ingroup in phylogenetic estimation theory. Alternatively, it refers to a method for determining trait polarity based on species group. By including the outer group in the analysis, the position of the inner group root (a common ancestor of the entire inner group phylogenetic tree) can be determined. In the early theory of diversification taxonomy, first the polarity of the trait state of the inner group, that is, the primordial trait state and the derivative trait state, was judged based on the trait state of the outer group, and then it was decided beforehand. Organize species that share a derivative trait state into a monophyletic group based on the polarity of the trait. Out-group comparison is widely used, among other things, as the main method for discriminating the polarity of morphological traits. The rationale is called the most conservative principle.The most conservative estimation of the virtual trait state (criteria for polarity discrimination in the inner group) on the branches connected to the roots of the inner group is made by the most conservative estimation. Because there is. Maddison, Donoghue & Maddison
(1984) and Swofford & Maddison (1987), the systematic estimation of the inner group based on the outer group comparison is logically equivalent to the most conservative systematic estimation that does not perform polarity judgment for the group including the inner group and the outer group. Proved that. Therefore, it has become possible to estimate the most conserved strains based on branch taxonomy even from molecular data such as restriction enzyme cleavage sites and nucleic acid base sequences that are difficult to determine polarity.

In the present specification, the term "co-derived trait" means that a trait state (apomorphy) determined to be derivative is shared as a result of the estimation of the polarity using the outgroup comparison or the like. The theory of branching taxonomy argues that only covalent traits provide information to infer phylogenetic relationships. This is because it is possible to assume a direct common ancestor that gave rise to a species group that shares the deduced trait. Therefore, the shared derivation trait serves as a clue for constructing a monophyletic group (more precisely, a complete phylogenetic group). here,
If a trait distribution mismatch occurs, the derivative trait state of some traits must be considered to be homoplasia that evolved in separate branches rather than from a common ancestor. The validity of the hypothesis of a shared derivation trait is verified by the consistency with the phylogenetic hypothesis selected based on the most parsimony principle, that is, the bifurcation diagram.

In the present specification, "polarity" refers to the evolution direction of the transition order between the trait states of a certain trait. Therefore,
The polarity depends on the model of transition order. The transition order of the trait state reflects the trait of the trait data used for lineage estimation. It is not uncommon for qualitative morphological traits to assume a linear or branched transitional order. On the other hand, most of the molecular data such as nucleobase sequences and restriction enzyme cleavage sites,
Unordered among trait states that cannot be ordered
d) It is a trait. Divergence taxonomy and evolutionary taxonomy require polarity estimation of trait evolution prior to phylogenetic analysis. Outgroup comparison, fossil records and information on ontogeny are used as criteria for estimating polarity, but the most widely used is outgroup comparison.

Evolution of SINE In order to effectively use SINE in the strain estimation, the isolation / identification method and the species discrimination method according to the present invention, SINE is used.
It is important to understand how has evolved. SINEs can be divided into families based on sequence similarity and subfamilies based on characteristic nucleotide presence and / or deletion, as described in detail below. The fate of SINE once inserted in the genome depends on various factors (Schmi
and Maraia 1992) and a mutation that prevents amplification that accumulates in SINE. Furthermore, for the amplification of SINE, R coded in LINE
Since Tase is required, if LINE loses its amplification ability in the genome and dies, it will cause SI in the organism.
The death of the NE is also meant at the same time (Okada et a
l., 1997).

Regarding how SINE is amplified in the evolution process in the genome, there have been two studies of SINE evolution so far.
Two allelic models have been proposed: a master gene model and a multi-source gene model. Master gene model (Fig. 2
(A)) is that only one or a few "master" loci are the source of all progeny copies, which are not capable of replicating on themselves. In this scenario, its amplification over time depends entirely on the conditions and activity of its master gene. On the other hand, multi-source gene model (Fig. 2 (B))
Then, since the offspring can have the same amplification ability as the parent copy, they function as "multi-source genes" over the course of evolution. In the latter model, amplification rate is a function of the rate of increase or decrease in total copy number from all of the source genes. The master gene model is rodent ID SINE (Kim et al, 1994)
And early studies of human Alu repeats (Shen et al., 1991)
Proposed by empirical evidence from. However, subsequent detailed Alu sequence studies and comparative studies from various other taxa now make the most sense to interpret that most SINE family amplification is caused by the multisource gene model. (Matera et al,
1990; Schmid and Maraia, 1992; Leeflang et al, 199
2; Kido et al., 1994; Takasaki et al. 1994; Shedloc
k and Okada, 2000). In practice, subfamilies characterized by the presence and / or deletion of characteristic nucleotides are:
It represents each source gene.

The period between the birth and death of SINEs is the period of activity of SINEs within the host genome, and their longevity is directly related to their use in taxonomy and as tools in the present invention. If the average degree of sequence dissimilarity between members of the SINE subfamily is small, it is reasonable to assume that the subfamily is fairly young and still actively amplified in the host. If the average sequence difference between members of the SINE subfamily is large,
It is reasonable to assume that the subfamily is relatively old and is already inactive or dead in the host. However, the diagnosis of common ancestors between host taxa using SINE insertion is possible only within the active lifespan of a given subfamily. The basic principle of this SINE method will be further described in the "Procedure" section below.

SINE Insertion Kinetics and Feature Theory The key to why SINEs are powerful taxonomic tools is that they are irreversibly independently inserted into the host genome (Murata et al. , 1993; She
dlock and Okada 2000). Since there is no known mechanism for specifically removing SINE from the genome, and the probability of two elements being inserted into the exact same locus or being excised exactly from the same locus is extremely low, the present invention Have SINs at the same locus in two different taxa
The presence of E can be considered as a polarized derivative phenotype in its genome. This defines a clade or monophyletic group in the strict sense of the method of Hennig (1966), which uses only covalently derived traits or shared derivational traits to construct the phylogenetic hypothesis. In this clade, known ancestral conditions, or lack of SINE insertions at a given locus, uniquely identify the outgroup without the need to establish a characteristic polarity via a competing method that produces well-known artifacts. (Hendy and Penny, 1989) (see Figure 3).

Methods for Isolation and Characterization of SINEs The following are strategies for isolating novel SINEs from genomic libraries, screening methods for them, sequencing of clones and SINE family-to-subfamily characterization, representative Copy number quantification in various host taxa, as well as the definitive diagnosis of SINE insertion patterns that provide phylogenetic information.

Procedure Species Selection Method for Generating Genomic Libraries The SINE method consists of the following basic steps: 1) Generation of a genomic library from the selected species; 2) Selection of clones containing the SINE locus. Isolation; 3) Determination of the DNA sequence of the clone; 4) Polymerase chain reaction (PC) within the flanking sequences of the SINE locus.
R) primer design; and 5) SI between related species of interest
PCR diagnostics for the presence or absence of NE. SINE
Once established, a law can provide deterministic results quickly and reliably, but establishing and establishing its procedures and conditions requires considerable time and effort.

Consider, for example, the case of determining the phylogenetic relationship between closely related species A, B, C, and D. The actual phylogenetic tree of the above species is shown in Figure 4 (A). In this case, seed D
, But when selected as a host from which to isolate SINE, no SINE locus providing phylogenetic information is obtained. Species D is a SIN inserted within the common ancestors and older origins of the four taxa of interest.
This is because it includes the E locus. The locus inserted in all four ancestors is shown as SINE3 in FIG. 4 (B). The pattern of the PCR electrophoresis gel showing the presence (+) or absence (-) of SINE3 insertion is shown in the lower part of FIG. 4 (C). In order to isolate SINEs that provide phylogenetic information, species A or species B capable of providing the three SINE loci shown in Figure 4 (B), SINE1, SINE2, and SINE3, must be selected. I have to.
Each of these loci defines a clade, or monophyletic group, that shares a common ancestor in the evolutionary history of these species.

Of a new SINE family from a particular species
Isolation Method SIN in a specific species, eg, species A in Figure 4 (A)
When the E family is completely unknown, it is necessary to newly isolate and analyze the SINE family from its genome. There are two methods by which new SINE families can be isolated from selected species. 1 is total genomic DNA transcription in vitro (Endoh and Okada, 1
986), and others include genomic DNA sequencing of about 60 Kbp and above facilitated by a new high-throughput automated DNA sequencing method (Nikaido and Okada, unpublished).

In Vitro Total Genomic DNA Transcription Most SINEs are known to be derived from tRNA, therefore SINE has an internal promoter for RNA polymerase III. SIN
Although E is extremely rarely transcribed in vivo, SINE can be easily transcribed from naked DNA in vitro. H using a radiolabeled precursor nucleotide, eg alpha P32-GTP
When transcribing certain total genomic DNA in eLa cell extracts, radiolabeled RNA is usually transcribed. In some cases, these radiolabeled transcripts form clear bands when they are subjected to gel electrophoresis.
(Endoh and Okada. 1986; Matsumoto et al. 1986).

This radiolabeled RNA can be used as a probe to screen a genomic library from a selected species of interest. If this transcript forms a clear band in the gel, it represents the actual SINE family. This is because all of the same transcripts from each locus of a given SINE family assemble to form distinct bands. Even if unclear bands are generated from genomic DNA, they can be used as probes for screening. However, in the latter case, the transcript may represent multiple SINE families.

From the experience of the inventor of the present invention, when SINE having a copy number of 10,000 or more is present in the genome of vertebrate and / or invertebrate, they are detected by in vitro transcripts of total genomic DNA. Can be done. FIG. 4 shows whole-genome DN from selected animal species.
Some example patterns of transcripts from A are shown (Endoh
and Okada, 1986).

Greater than 60 kbp by automatic sequencer
Sequencing of Kina Genomic DNA According to the experience of the present inventors, the copy number of the SINE family in the genome is usually over 10,000. Assuming that the entire genome is 3 × 10 ⁹ bp in length and the size of a certain SINE is 300 bp, such SI
The NE family will occupy 0.1% of its genome (eg, 300x10 ⁴ = 3x10 ⁶ ). Thus, sequencing over 60 kbp or more could find two independent SINE sequences within this kind of randomly isolated DNA fragment (eg 600x100 / 0.1 = 6x10 ⁶ ).

This has become easier to achieve in the laboratory with access to the latest models of high throughput automated DNA sequencers. For example, a novel SINE family has recently been characterized from the elephant genome and it has been shown that this novel SINE is distributed among all species of Afroteria (Nikaido and Okada, unpublished). This method can be applied to the genomes of all mammals and perhaps most vertebrates.

Accurate identification of the SINE family
Method of Deducing Its tRNA Structure After sequencing multiple copies of the repeat unit according to the method described above, they can be aligned and the consensus sequence of the repeat family can be deduced. Since there are many repetitive sequences in the genome besides the SINE element,
Proper diagnosis of the sequence is essential. Since most SINEs are known to be from tRNA, they contain a promoter for RNA polymerase III. The RNA polymerase III promoter is a conserved sequence block, having the characteristics of a first promoter and a second promoter separated from each other in its genome. This second promoter is highly conserved and can be easily recognized empirically.

Consider now an example of CHR-2 SINE. The tRNA-like structure of these elements can be established as follows (see Figure 6): First, from the alignment of several sequences of CHR-2, CH
Construct a consensus sequence for R-2 SINE (Fig. 8
reference). 2. Visually search for the consensus sequence of the second promoter for RNA polymerase III. This sequence is 5'-GT (or A) TCG (or A) -3 '. There are no exceptions to this motif when screening this promoter. When this motif is present, it creates a stem-loop structure containing this second promoter sequence. The number of bases in the loop is 7 and the number of base pairs in the stem is 5. Even if all of the bases in the stem region do not form base pairs, they are placed at the appropriate positions within the tRNA, as shown in Figure 6 (A).

3. Consider a unit consisting of 5 bases 5 ′ upstream from the stem region. This is because, in protoplasmic class ItRNA, this extra loop region consists of 5 bases. In the case of CHR-2 SINE,
The sequence of this unit of 5 bases is 3'-CAGGG-5 '.
The 3'-PyPyPuPuPu-5 'sequence is typical of this extra loop in some tRNAs (Sprinzl et al. 198).
7), and thereby, the SRNA tRNA origin can be deduced (see FIG. 6 (B)). 4. The next 5 bases that form the aminoacyl-stem region of the tRNA structure are considered another unit. in this case,
It is 3'-GACGT-5 '(see FIG. 6 (C)). 5. The next 7 bases forming the anticodon-loop region of the tRNA structure are considered yet another unit. In this case it is 3'-AACCGTC-5 '. AA at its 3'end
The 3'-TC-5 'residue at the 5'end of the residue is a good indicator of the origin of this SINE tRNA. This is because these bases are highly conserved in most tRNAs (Galli et al., 1981). This is this SI
It supports the tRNA origin of NE (see Figure 6 (D)). 6. Usually, the next 5 bases are considered another unit and they should base pair with the 5 bases assigned to the anticodon-stem region (FIG. 6 (C)). In this case, the sequence is 3'-CGTCT-5 ', and only its first four bases match well with its anticodon-stem region partner. In order to correctly align this unit, a deletion is placed at the position of the first base 3'of the anticodon-stem (see Fig. 6 (E)). 7. Next, a stem-loop structure for the D region of this tRNA-like structure is constructed. The number of bases in this stem and loop is usually 4 and 8, respectively, but especially tR of SINE
In the NA-like structure, it can vary by 1-2 bases. Apparently, some base pairs in CHR-2 order do not form meaningful secondary structure. In this case, pay attention to the first promoter region. The most prominent feature in the first promoter region is the presence of two G's in that region. Another feature is G and 1 at position 15 in this loop (depending on the tRNA numbering system. The same applies hereinafter).
It is A in 4th place. The A at position 14 is the first base in the loop on the 5'side. Therefore, these bases are placed at the corresponding positions of the loop of this tRNA-like structure (see FIG. 6 (F)). It is well known that T, which is the first base in FIG. 6 (F), is highly conserved in all tRNA molecules. 8. The tRNA-like structure of CHR-2 SINE is shown in FIG.
The sequence shown in (F) can be deduced by combining it with another sequence for this family (FIG. 7 (A)). 9. Next, B in the GenBank DNA database
Using the LASTN program (Altschul et al. 199
0), searching for similarities between CHR-2 SINE and actual tRNA. In this example, tRNA Glu most closely resembles the sequence of CHR-2. FIG. 7 (B) shows human t
The secondary structure of RNA Glu is shown.

Specializing the SINE family as a subfamily
Method of conjecture It is assumed that a SINE family has been characterized in phylogeny within the genome of species A, and it is unknown when this SINE family was first generated during evolution. Further assume that the SINE family was first generated in the old common ancestor of all taxa of the clades Y in FIG. In this case, the copy of SINE present in the genome of species A contains the old SINE amplified at t and the young SINE amplified at u in FIG.
When screening a genomic library of species A with this SINE family consensus sequence,
Both the old SINE and the new SINE described above can be isolated. Since only the phylogenetic relationships of species A, B, C, and D are sought, it is inefficient to look at all times of an isolated SINE amplification event. Rather, it is amplified near the branch of species D and includes branch group X in FIG.
It is much more efficient to try to isolate the SINE locus across all branches of one taxon.

As mentioned above, SINE and LINE are believed to be amplified according to the multisource gene model.
(Schmit and Maraia 1992; Smit et al. 1995). If a source gene is mutated and successfully amplified during evolution, this mutated source gene can be recognized as a subfamily within its corresponding SINE family (Britten et al. 1988). ; Jurka et al.
1995). Subfamilies are amplified at some stage of evolution. Therefore, one subfamily is species A, B,
When amplified only in the common ancestors of C and D, copies of this subfamily can be effectively used to determine the phylogenetic relationship of these four clade.

The SINE family consensus sequence in species A was established as part of the above procedure, and the design of one PCR primer at the 5'end of the SINE sequence and another primer in a conserved region near its 3'end. To enable. The sequence amplified by this primer set includes the entire SINE sequence. This primer set is a genomic DN derived from species A
It can be used to amplify multiple copies of SINE by PCR with A. PCR of this reaction
The product can be cloned into an appropriate vector DNA and sequenced. At this point, the P
Sequencing 100 copies of SINE from a CR product is not a difficult task. By aligning these sequences, one can identify the characteristic nucleotides or possible deletions that represent a subfamily of this SINE family (see Figure 10). A characteristic nucleotide is defined as one that is cooperatively altered at three or more nucleotide positions within a particular subfamily and that can be distinguished from the spontaneous mutations accumulated in the random in the SINE sequence during evolution. To be done. Only after successfully characterizing the subfamily based on the presence of characteristic nucleotides and often specific deletions can the taxonomic distribution of the given subfamily be examined by dot-blot hybridization or PCR. Like

CHR-2 SINE Subfamily FIG. 8 shows Cetaceans and Subpopulations of Hippopota.
muses), and an alignment of copies of the CHR-2 SINE family (Shimamura et al. 1997) originally characterized as present in the genome of the Ruminants. It is easy to see that there are six subfamilies in CHR-2 SINE. FL (full length), MDI (central deletion I), MDII due to the presence of the deletion
(Central deletion II), and the shortest group were characterized. next,
This shortest group can be divided into DT (deletion type), CD (whale deletion type), and CDO (whale deletion tooth whale specific) subfamilies. FIG. 11 shows the alignment results of the consensus sequences of these subfamilies. SEQ ID NO: 1 is an upper consensus sequence, SEQ ID NO: 2 is an FL consensus sequence, SEQ ID NO: 3 is an MDI consensus sequence, SEQ ID NO: 4 is an MDII consensus sequence, SEQ ID NO: 5DT consensus sequence, SEQ ID NO: 6 is a CD consensus sequence, and SEQ ID NO: 7 represents the CDO consansus sequence.

FIG. 12 shows the results of dot-hybridization experiments using probes specific to CD, CDO and other subfamilies. The results clearly show that the CD subfamily is specific to the genome of Cetaceans (dental and bearded whales) and the CDO subfamily is specific to the genome of cetaceans. Therefore, SINEs belonging to the CD subfamily are useful for estimating the phylogenetic relationships of Cetacea, especially bearded whales, while SINEs belonging to the CDO subfamily are useful for estimating phylogenetic relationships of dental whales. It is useful. The distribution of copies of the CDO subfamily also suggests a monophyletic order of the suborder Whales, which includes sperm whales, which was one of the most controversial points in taxonomic biology of mammals.

Flanking SINE PCR Given after the isolation and sequencing of the SINE loci from species A belonging to a subfamily generated in the common ancestor of clade X shown in FIG. PCR experiments can be performed to diagnose the presence or absence of insertions at the locus. A primer sequence is selected by looking at the flanking (flanking) sequence. Care must be taken in the design of the primers for the formation of secondary structure folds and for tandem annealing between upstream and downstream primers. This can be easily checked using various standard software programs written to facilitate PCR primer design available commercially or through the internet. The melting temperature of the oligonucleotide primer is set to around 55 ° C. Therefore, the annealing temperature for PCR is
This temperature must be based when optimizing the amplification of orthologous loci from C and D. Occasionally, the accumulation of mutations in the primer binding region for species B, C, and D prevents efficient primer-template annealing during this reaction. In this case, the annealing temperature should be reduced to about 45-50 ° C. If the PCR product is not amplified by PCR with the first primer, new PCR primers should be designed, with additional attention to potential artifacts that may reduce the efficiency of PCR. FIG. 13 schematically shows the principle of flanking SINE PCR.

FIG. 14 shows an example of the PCR result. In addition, the results of hybridization experiments performed using the same filter using two different probes are also shown. FIG. 14 (A) is a PCR pattern that provides evidence that sea dolphins are monophyletic. Because the PCR products from sea dolphins have the expected fragment size containing the inserted elements, while fragments from other tooth whales are Mago 19
Because it has the expected fragment size that lacks the insertion at.
FIG. 14 (B) shows the hybridization experiment using the SINE probe, while FIG. 14 (C) shows the hybridization experiment using the flanking DNA of the above loci. This latter experiment was performed to demonstrate that orthologous loci were faithfully amplified by PCR in species other than Magnolia that the loci were originally isolated and characterized from.

Interpretation of PCR data When examining relatively recently diverged species, orthologous S
The flanking sequences at the INE locus are faithfully conserved, and typically do not pose problems that interfere with PCR diagnosis. However, PCR is more often missed when examining relatively old divergent taxa, making interpretation of experimental results difficult. In unsuccessful PCR, SINE-minus data appears. That is, there is a successful PCR amplification at a given locus indicating the absence of SINE insertion. The unsuccessful PCR is
As is (eg,
"?") Is coded. There should be ancestral polymorphisms and subsequent incomplete phylogeny when there are inconsistent insertion patterns between the independent loci examined.

Distribution of SINEs in the Mammalian Genome Generally, most mammals have large amounts of SINEs. They include hybridization between the species investigated.
Based on the pattern (eg, CHR-2 SINE distribution in FIG. 12), it is clearly eye, suborder, superfamily, family, genus, or species specific. Such empirical evidence is based on SI
It shows that the NE family is newly generated in many ancestral mammalian strains. However, its generation mechanism is not fully understood. The reason for such large numbers of SINEs or retroposons in the mammalian genome may be that the RTase encoded by the mammalian L1 alters its template recognition specificity in the mammalian common ancestor. . This allows the recognition of the poly A tail required for the retroposition present in many LINEs, which strictly recognizes the 3'tail responsible for the formation of the stem-loop structure (Ohshima et al. 1996; Oka
da et al. 1997; Kajikawa et al.). Such a scenario could have allowed poly A-containing RNAs to become pseudogenes via L1 RTases in the mammalian genome.

FIG. 15 shows the recently proposed mammalian phylogenetic tree (Waddell et al. 1999; Cao et al. 2000; Ni
kaido et al. 2000). The mammalian SINE family characterized to date is listed above in FIG. Simply put,
The oldest SINE family distributed in the entire mammalian genome is MIR (Smit and Riggs 1995; Jurka et al.
al. 1995). Although the Alu family is clearly specific to the primate genome, its distribution among its related species, such as the cynomolgus monkey, has not been investigated in detail. Rodent B1,
B2 and ID are specific to the rodent genome. The rabbit C family has been reported within the Genome of the order Lagomorpha, but its distribution among related species has not been reported. SINE family present in the Artiodactyla genome, for example,
CHR-1, CHR-2, CHRS, CHRS-S, P
RE-1 and Bov-tA have been investigated in detail
(Shimamura et al. 1997; Shimamura et al. 1999; Nik
aido et al. 1999). CanSINEA was first reported from the Canidae genome (Minnick et al. 1992; Coltma.
n and Wright 1994), and was subsequently shown to occur during evolution within many other carnivorous genomes (van der Vlugt an.
d Lenstra 1995). An equine SINE, named ERE family, was reported and its distribution examined (Sa
kagami et al. 1994; Gallagher et al. 1999). The bat SINE family is Borodulina and Kramerov (199
9) and named VES, and other bat SINE families have recently been characterized
(Kawai et al.). The elephant SINE family was recently isolated and shown to be distributed among Afrothelia species (Nikaido and Okada). Where the derived tRNA
Or SINE that has a completely different array
SINEs that are derived from the same origin but have a diagnostic mutation can be identified as mily by Type or subfamily
Are distinguished as. It is considered that a certain SINE explosively increases its copy number at a certain time, and the time of its explosive amplification and the time axis in the evolution of the whole organism are related to their phylogenetic relationship and the distribution of SINE.

Importantly, although many SINE families have been isolated to date within the mammalian genome, they have not yet been characterized to their subfamily structure. Therefore, according to the present invention, its subfamily structure will be clarified in the future, and a new mammalian SINE family or subfamily can be further acquired.

New amplification of SINE: fixed and short term
If the species divergence SINE is very newly amplified in the evolution of the genome and is not fixed between populations of one species, its status as a common trait is unstable and it is used for phylogenetic tree generation. Should not be. However, such SINE distributions can be used for analysis of population structure (eg, Hama
da et al. 1998). If species divergence occurs in the short term, that is, before most SINEs are fixed among populations via genetic drift, ancestral polymorphisms and subsequent incomplete phylogenetic classification may lead to inconsistent SINEs. Create an insertion pattern. This phenomenon, combined with the irreversible nature of the SINE retroposition, provides a bias for using SINE to investigate historical patterns of phylogenetic taxonomy in explosively derived taxa. If the ancestor has a polymorphism and the SINEs are incompletely distributed to the offspring and create inconsistent insertion patterns, the most conservative method, which is one of the phylogenetic tree construction methods, is used at each locus. It is useful to evaluate the SINE character matrix for the presence or absence of insertions. Unfixed, polymorphic SI
Although NEs are not useful for high-level phylogenetic relationships, they are known to be excellent taxonomic tools for population analysis (Deininger and Batzer,
1994, Stoneking et al, 1997; Hamada et al, 199
8). Therefore, according to the methods described herein,
By identifying and characterizing the SINE family or subfamily, it is possible to carry out a method for determining the species of animals using the SINE family or subfamily.

Function and value of flanking sequence (useful
Sex) Information on the nucleotide flanking sequences within the locus examined for SINE insertions is useful. Although the insertion data is of course useful for creating a phylogenetic tree and for use in the method according to the present invention, the integration of the insertion sequence with the flanking sequence does not affect the length of the branches between clades defined by the independent SINE insertion case. Information about the quality (Lum et al, 2000; Shedlock and Okada, 2000). Furthermore, since the flanking sequences associated with a given insert are literally linked, the consistency between the SINE-derived topological versus flanking sequences is important for assessing the basic hypothesis of irreversible insertion at each locus. Provides numerous approaches (Lum et al, 2000). Independent SIN
There may be apparent discrepancies between the phylogenetic trees if there is homoplasia at the E locus, or trait inconsistency. Such an approach is emerging as a new dimension of SINE analysis, and SINE
It provides the basis for enhancing the statistical evaluation of law.

[0047]

EXAMPLES The present invention will be described in detail below with reference to examples. However, the technical scope of the present invention is not limited by the following examples.

Materials and Methods Buffers, Enzymes and Other Reagents Various buffers used for manipulating nucleic acids, enzymes, media used for culturing E. coli, and other reagents are Wako Pure Chemical Industries, Ltd., Si.
Sambrook et purchased from gma, Difco, FMC
It was adjusted with reference to the reference of al. (1989). Various restriction enzymes, modification enzymes, and vector DNA were purchased from Takara Shuzo Co., Ltd., Toyobo Co., Ltd., and Amersham Life Sciences. The radioisotope was purchased from Daiichi Pure Chemicals Co., Ltd. Regarding PCR primers, those ordered by Oligo Express (Amersham Pharmacia Biotech Co., Ltd.) for synthesis were used.

Genomic DNA Various samples (tissue or DNA) used in this study are shown below. The whales and cloven-hoofed English names used in the analysis,
Table 1 below for Japanese and scientific names:

[Table 1] Shown in. Bactrian camels extracted DNA from muscle tissue provided by Dr. Minoru Yoshida of the University of Tokyo School of Medicine. For pigs, the DNA stored in our laboratory was used as it was. The peccary used the DNA provided by Dr. Hiroshi Yasue of the Livestock Experiment Station of the Ministry of Agriculture and Fisheries. Javan deer used DNA provided by San Diego Zoo, USA. The DNA provided by Isao Munechika of Chiba City Zoological Park was used for Axis deer, Amemequilin, Sable antelope, Japanese serow, Markhor, Muflon, and hippopotamus. The sheep used was extracted from the liver provided by Mr. Yasushi Mukai of Ueda Meat Inspection Center, Nagano Prefecture. For cattle, DNA was extracted from the kidneys provided by the Sagami Branch Office of the Meat Hygiene Research Center in Kanagawa Prefecture and used.

As a sample of narwhal and great whale, a tissue piece considered to be muscle was obtained from Dr. Masaki Miya of the Chiba Prefectural Central Museum, and DNA was extracted and used. Regarding the sample of Great Gray Whale, it is known that it belongs to the genus Great Gray Whale of the family Red Whale, but the species name is unknown. Other samples except river dolphins were used by extracting DNA from various tissue pieces provided by Dr. Hidehiro Kato of the Large Whale Research Laboratory, Oceanic Fisheries Research Institute, Fisheries Agency. For samples of river dolphins, see Dr. Robert L. Brownell, Center for Southwestern Fisheries Science, USA.
Jr .: Chief Marine Mammal Division, Southeast Fish
eries Science Center, PO BOX 271, LaJolla, Calif
ornia 92038), I had the procedure for acquisition in Japan carried out. Healy H. Hamilton of California State University, Berkeley, for the subcutaneous tissue slices of the Amazon dolphin and the liver tissue slices of the La Plata dolphin based on it.
DNA was extracted by the donation from Dr. Gandhiskawa dolphins were provided with dried bone samples by Mr. Tadashi Yamada of National Science Museum and extracted DNA. As for dolphins, fresh blood was collected from individuals kept in the aquarium of the Institute for Aquatic Biological Research, Chinese Academy of Sciences, heparinized on the spot, transported to the laboratory, and DNA was extracted immediately. Extracted DNA
Is currently stored in a Chinese laboratory because it cannot be brought into Japan.

Extraction of Genomic DNA Genomic DNA was extracted from the tissues of the animal species used for analysis by a simple method (Rapid method; method using Proteinase K).
The extracted genomic DNA was used as a template for library preparation and PCR. Also, they are stored at 4 ° C.
Cut tissues (liver, muscle) of whale and cloven-hoofed animals stored in a -40 ° C freezer into a few millimeters square with a scalpel, transfer to a microtube, weigh the tissue, and then 1X into the microtube. TNE buffer (10mM Tris-HCl (pH8.0),
500 μl of 100 mM NaCl, 1 mM EDTA (pH 8.0)) was added. Finely crush the tissue pieces in the tube using a Pasteur pipette, and then add 1X Lysis buffer to the tissue and TNE buffer.
(20 mg / ml Proteinase K, 2% SDS, 10 mM Tris-HCl (pH 8.
0), 150 mM NaCl, 10 mM EDTA (pH 8.0)) was added, and the mixture was inverted and stirred, and then incubated using a water bath at 55 ° C. to completely dissolve the tissue piece. Add the same amount of phenol as the solution and add 1-2 with a belly dancer (200-300 rpm).
Stir for hours. After centrifugation (room temperature, 12000 rpm, 5 min),
The supernatant was transferred to another 2.0 ml microtube. In the same manner, phenol / chloroform extraction and then chloroform extraction were repeated, and 0.1 volume of 3M sodium acetate and 2 volumes of ethanol were added to the final supernatant. At this time, in many cases, fibers were confirmed, and after rinsing with 70% ethanol, an appropriate amount of TE buffer was added to dissolve the pellet. From a blood sample of a northern dolphin
DNA extraction is performed using a commercially available column (QIAamp Tissue / Blood Kit C
at. No.29306: Qiagen Co., Ltd.) was used.

Flanking PCR (mainly for increasing homologous loci)
With flanking PCR, primers are designed in the flanking regions on both sides (upstream and downstream) so as to sandwich the SINE sequence.
This means performing PCR using DNA as a template. The primers used at that time were designed to have a length of about 17 to 30 nucleotides and a Tm (Melting Temperature) value of about 55 ° C. Regarding its design, CPrimer (Ver.1.08, Bristol and Anderso, which can be downloaded on the Internet as Macintosh version free software)
n (1995)) was used. Reaction composition: Template DNA (100 ~
500 ng); 10 X PCR Buffer (100 mM Tris -HCl (pH 8.
3), 500 mM KCl, 15 mM MgCl ₂ 5 μl; dNTP Mixture
(2.5 mM each) 4 μl; Forward and Reverse Primer (5
pmol / μl) 2 μl each; TaKaRa TaqTM (5 U / μl) 0.25
μl; adds ddH ₂ O up to final volume 50 μl. Reaction conditions: 94 ℃ (Pre-denature) 2-3 min .; and 30 cycles
s of 94 ℃ (Denature) 30 sec .; 45 ℃ ~ 60 ℃ (Anneal
ing) 1 min; 72 ℃ (Extension) 30 ~ 90sec. At this time An
The temperature of the not only the primer Tm value,
The optimum temperature was sought by changing the loci according to the ease of increase of the loci.

Colony PCR Colony PCR is a very convenient method for selecting whether or not a target DNA fragment is contained as an insert in a clone when performing subcloning. Conventionally, plasmid DNA was prepared by Miniprep and then digested with a restriction enzyme to confirm the presence or absence of the insert.However, it is possible to check this process only by PCR, so it takes a lot of time and labor. It leads to shortening. First, lightly peck the colonies of E. coli on the plate with a toothpick.
Simply rub it onto a 5 ml PCR microtube and use it as a template to perform ordinary PCR in a reaction system of 20 μl. The primer used at this time is prepared based on the sequence specific to the vector. Vector DNA that serves as a template because the cell membrane of E. coli is destroyed during heat denaturation in the PCR reaction
This PCR can be performed by dissolving in the solution. As will be described later, after confirming the presence or absence of the insert by this PCR, this PCR product can be directly used as a template for sequencing by using an ABI sequencer, so that the sequence of the insert can also be performed immediately after the insert check. It was considerably shortened.

Determination of nucleotide sequence The following sequencer and sequence reaction kit were used to determine the nucleotide sequence. LI-COR dNA Sequencer
Sequence using (Model 4000): SequiTherm EXC
ELL ™ II Long-ReadTM DNA Sequence Kit-LC (Ca
t. No. SE7701LC, EPICENTRE TECHNOLOGIES); ABI
Sequencing with PRIZM ™ 310 Genetic Analyser: BigDye Terminator Cycle Sequencing FS Rea
dy Reaction Kit (P / N 4303152, Perkin Elmer).
When the subcloned plasmid DNA was used as a template for the sequencing reaction, it was prepared using the SDS-Alkaline and Mg precipitation method. When determining the base sequence of the locus containing the SINE sequence isolated by screening, M4 and RV on the vector sequence, and an oligonucleotide primer (fluorescein (aloca ( Co., Ltd.)
Was used. When PCR products are directly sequenced, add Srimp Alkaline Phosphatase (SAP) 2U /
μl 0.5 μl; Exonuclease I 10U / μl 0.5 μl (both from Amersham Life Sciences) were added directly to the plate at 30 ° C at 37 ° C.
After incubating for a minute to decompose the oligonucleotide, it was incubated at 85 ° C. for 15 minutes to inactivate the enzyme, which was used as a template for the sequencing reaction.

Preparation of genomic library About 50 μg of sucrose gradient genomic DNA was completely digested with an appropriate restriction enzyme (Hind III was mainly used in this study), phenol / chloroform extraction and ethanol precipitation were performed. , 200
It was dissolved in μl of TE buffer. 15 ml plastic tubes for ultracentrifugation (Centrifuge Tubes-50 Ultra
-Clear (TM) Tube 14 X 89 mm, Order No. 344059:
Create a 10-40% sucrose gradient on BECKMAN, add the DNA solution onto it, attach to a rotor, and centrifuge (25000) with an ultracentrifuge (L8-70M, Serial No. 7C869: BECKMAN). rpm., 15 ° C., 15 hours). After centrifugation,
Fractionation (about 250 to 400 μl / fraction) was carried out by dropping 10 to 15 drops into a 1.5 ml microtube. The fraction is 0.7
Electrophorese on a 1% agarose gel, select 4 to 6 fraction tubes so as to sandwich the fraction containing the fragment of about 2 to 4 kbp, and perform ethanol precipitation.
It was dissolved in buffer. 0.7 to 1% agarose electrophoresis was performed again to determine the fraction used for library preparation.

Construction of plasmid library In this experiment, pUC18 / HindIII or pUC19 / Hin was used.
Using only the plasmid library using dIII,
No phage library was created. Since it was known from our experience in our laboratory that it is possible to obtain a sufficient amount of positive clones without using a phage library, because there is a sufficient copy number of SINE in the whale and artiodactyl genomes. To save time, we used a plasmid library with relatively few steps. The plasmid library was prepared as follows. The composition is TaKa
Ra Ligation Kit Ver.1 A solution, 12 μl; Same B solution, 1.5 μ
l; Vector DNA (pUC18 or 19 / HindIII ~ 100 ng / μl), 0.
5 μl; Insert DNA (Genomic DNA Sucrose Density Gra
dient Fraction), 1.0 μl: The above solutions were mixed in a 0.5 ml tube, and then left on a cool block at 16 ° C for 30 minutes or longer. This genomic library is stored at -20 ° C.

Flow until isolation of clone containing SINE sequence FIG. 16 shows a flow of an experiment in the SINE method. Screening Preparation of membrane used for screening Add the above-mentioned genomic library mixture to a 0.5 ml tube.
An appropriate amount of 1 μl of E. coli (E. coli JM105 strain) competent cells was added and mixed, and the mixture was allowed to stand on ice for 30 minutes. 42
After heat shock at ~ 45 ° C for 45 seconds,
L / amp / X-gal / IPTG incubated at 37 ° C
The plates were plated and left in a 37 ° C incubator overnight. 20 colonies per plate
It was adjusted so that the number of cells was 0 to 300, and a library for 10 to 20 plates was spread. Colonies that grew on this plate were collected using a nylon membrane (Colony / Plaque Screen ™).
NEF-978: Transferred to NEN Research Products, and then denatured solution (0.4 M NaOH, 0.6 M NaCl), neutralized solution (1 M NaCl, 0.5 M Tris-HCl (pH7.0)) for about 3 minutes. I left it. The membrane was well dried after draining. After transferring the colonies, the plate was incubated at 37 ° C. so that the colonies were sufficiently grown again so that the positive clones described later could be easily picked up.

Preparation of probe used for screening Oligonucleotides or PCR products were used for screening. The oligonucleotide sequences actually used are shown in Table 2 below:

[Table 2] Shown in. The method of preparation is first for oligonucleotides: ddH2O 27-37 μl; Oligo Nucleotide (5 pmol / μl)
1 μl; 10 X T4 Polynucleotide Kinase Buffer 5 μl;
T4 polynucleotide Kinase 2 μl; [γ-32P] ATP 5〜15
μl was mixed in a 0.5 ml tube and then incubated at 37 ° C. The sequences of the oligonucleotides used as probes are shown in Table 2 above. When using PCR products (Primer-Ex
tension method): Template DNA, 500 μg; Forward prime
r (12.5 nmol / μl) 2 μl; Reverse primer (12.5 nmol
/ μl) 2 μl dDTP Mixture (dATP, dGTP, dTTP) 2.5 μ
l; Heat denaturation of the above mixture at 95 ° C for 5 minutes, then at 55 ° C
It was annealed for 1 minute and then transferred to ice. Furthermore, the following reagents were mixed in order and then incubated at 60 ° C. for 30 minutes. 10X BcaBEST (trademark) Buffer 2.5 µl; BcaBEST (trademark) DNA polymerase 2 µl; [α- 32 P] dCTP. After the reaction, each probe was a NICK Column (Sephadex G-50 DNA
Grade: Amersham Pharmacia Biotech Co., Ltd.)
It was eluted with and purified. RI counts were measured with a liquid scintillation counter. (A Geiger counter was also used for simple measurement.)

Hybridization The membrane was placed in a hybrid bag, and an appropriate amount of prehybridization solution (6 X SSC, 1% SDS) was added thereto. The hybrid bags were packed with a sealer and incubated for 1 hour or more. Discard the solution and remove the hybrid solution (6 X SSC, 1% SDS,
1 X Denhart's solution, Carrier DNA (Shared Herrin
g Sperm DNA solution) was added, and then a preconditioned probe that had been heat-denatured at 95 ° C. for 3 minutes was added, and the hybrid bag was sealed. Then, it was incubated overnight (about 15 hours) in a 42 ° C water bath. After confirming the membranes by lightly rinsing counts with an appropriate amount of wash solution (2XSSC, 1% SDS), wash with a new wash solution by setting the water bath temperature to 55-60 ° C. I went.

Development After discarding the wash solution and lightly rinsing with fresh wash solution, the count was confirmed using a Geiger counter. Mount, membrane, X-ray film, Inten in dark room
The cassettes were placed in the order of sifier, and this was placed in a -80 [deg.] C. frizer for exposure. The X-ray film was taken out of the cassette in a dark room, and the X-ray film was placed in the order of a developing solution, a stop solution and a fixing solution for development.

Isolation of Positive Clone The position of the positive clone was confirmed by stacking the developed X-ray film and the plate plated with the library. Before performing Miniprep for colonies that are considered to be the positive clones, primers prepared based on the consensus sequence of the SINE sequence used for screening, not the sequence of the vector to confirm the presence or absence of the SINE sequence in the clone before performing Miniprep Colony with
PCR was performed. After that, a plasmid of a positive clone was prepared, and its neighboring region was sequenced using a LI-COR sequencer. Since the subsequent operation is as described above, it will be briefly described below.

First, a primer was prepared based on the flanking sequence, and flanking PCR was performed using genomic DNAs of various organisms as templates. And in many cases, it is possible to easily amplify the homologous locus for a closely related species of the species used for screening, but the homology of a distantly related group or divergence that is thought to have occurred in an old age. As for the locus, since there are more base substitutions accumulated in the vicinity region, annealing is not successful with the primer based on one sequence used in the screening, and it is often the case that amplification is not possible with the first flanking PCR. Was given. At that time, the nucleotide sequences of the amplified homologous loci of various species were determined, their consensus was taken into consideration, new flanking primers were prepared, and PCR was performed again.

In estimating the phylogenetic relationship between the whale and the Artiodactyla, Southern hybridization was performed after agarose gel electrophoresis to confirm whether the amplified band was a homologous locus. The process is shown below. After agarose gel electrophoresis, it was stained with an ethidium bromide (EtBr) solution, and the migration pattern was confirmed and photographed with a digital camera. Then denaturing solution (0.4 NaOH, 0.6M NaC
l) Membrane fully dipped in 2 pieces, filter paper 2 pieces in this order,
JK wiper, Kim towel (trademark), a dictionary as a weight, etc. were placed on the gel so that air bubbles would not get in between and left overnight (up to 15 hours). The membrane was left in a neutralizing solution (1 M NaCl, 0.5 M Tris-HCl (pH 7.0)) for about 15 minutes to neutralize it, and then dried sufficiently. Subsequent operations such as hybridization and development are the same as those in the above-mentioned screening, and will be omitted. In addition, since the nucleotide sequences of most of the loci were determined when estimating the phylogenetic relationships within the Whale Order, basically, confirmation by Southern hybridization was not performed.

Example 1: CHR- in the Artiodactyla genome
2 Distribution of each subfamily Regarding the origin of whales, its internal system is divided into morphological classification and molecular classification. There are three subspecies of the Whale eye, which includes many modern species, the Orchidacea and the Beard Whale, and the original Whale subgenus (also known as the Antarctic whale), which is thought to have been the ancestors of these. Divided into eyes
(Fordyce et al., 1994), the classification of modern whales is distinguished by whether they have teeth or not, as is clear from the classification name. However, whiskers can also see teeth until the very last stage of the development, and among the fossil species of the original whales, it is natural that they are in the intermediate stage between whales and whiskers. There is also a toothed whale with a tooth (that is, a toothed whiskers), so as a practical matter, it is not possible to assert the monophyleticity of a tooth whale only by the presence of teeth. This is because the "beard" of a whale is probably a shared trait when considered systematically, but the "tooth" trait of a toothed whale is considered to be a primitive trait. However, only whales have the ability to eco-locate, and the presence of melon bodies associated with them is one of the most famous traits that suggest monophyly of whales.

While the morphological classification strongly asserts the monophyly of each of the toothed whales and whiskers, according to the phylogenetic analyzes that have been conducted so far using molecules, most of them are toothed. It suggests that whales do not form monophytes. Among them, Milinkovith and Meye became the cause of big controversy.
In the phylogenetic tree proposed by the comparative analysis of mitochondrial gene sequences of r (1993), this analysis shows that the group of sperm whales contained in the whales is closely related to whiskers rather than other whales. That is, the whale is multi-system. Following this result, a number of molecular statistical studies were carried out, but most of them resulted in multiple or insolvent whales (eg, Arnason et al., 1994; Adachi).
et al., 1996; Smith et al., 1996: see Figure 17). Therefore, the species discrimination was performed using the SINE method to solve the following problems: Problem (1) Problems of monophyly of the whale; (2)
Phylogenetic relationships in all whale dolphins, including all dolphins; (3) Divergence age of each whale. The present invention has made it possible to clarify not only the determination of systematic relations, but also the problems of statistical analysis that have been advanced with absolute reliability up to the present.

As described above, the present inventor has roughly divided FL (Full Lengt) into CHR-2 existing in the Artiodactyla genome.
h), MD (Middle Deletion type), DT (Deletion Type), C
D (Cetacea Deletion type), CDO (Cetacea Deletion ty
pe Odontoceti) was found to exist. It is considered that the distribution of each subfamily reflects the phylogenetic relationship because the amplification time of each subfamily differs during evolution. An alignment of the consensus sequences for each subfamily is shown in Figure 18. As is clear from FIG. 18, each subfamily has Diagnostic base substitutions or deletions in them.

Dot hybridization was performed to confirm the distribution of each of these SINE subfamilies in the whale and Artiodactyla. For the probe used, an oligo probe was designed at a position where CD and CDO could be distinguished from each other (thick line of alignment), and a PCR product was used for FL. Figure 12 shows the results of dot hybridization.
Shown in. First, it was suggested that FL is distributed only in hippopotamus and cattle (representing ruminants) in all Whale orders and Artiodactyla, and not in the mammalian genomes added as pigs, camels and other subgroups. Next, CD is considered to be uniquely distributed only in the whale order and not in the even artiodactyla, strongly supporting the monophyly of the whale order. Furthermore, regarding CDO, since a strong signal was confirmed only in the whale, it is considered that this subfamily has undergone explosive amplification specifically in the suborder Whale,
This data supports the monophyly of the whale, which has been questioned by molecular analysis. However, if no signal is confirmed in the dot hybridization analysis, the possibility that the copy number was simply low in that strain cannot be ruled out, so it is not possible to infer the phylogenetic relationship based solely on the results. Therefore, we actually isolated the gene loci in which those SINEs were inserted in the whale eye genome and estimated their phylogenetic relationships.

Example 2-1: Insertion of SINE into the genome
Analysis of the internal lineage of the Whale eye as an index
CDs that are uniquely distributed in the whole whale eye from SINE,
Focusing on CDO, which is thought to be specifically amplified in the subgenus Odontocephalus, we have isolated loci containing SINE sequences belonging to these subfamilies from various genomic libraries of Whales. As described above, for efficient phylogenetic analysis, the distribution of subfamilies and their amplification timing can be associated and predicted. Regarding the phylogenetic analysis of sperm whales, the reason why such loci could not be isolated despite the continued search for loci suggesting monophyly I thought it was because I was paying attention to the CDOs that are distributed only in Japan. Therefore, we only screened the sperm whale genomic library using the sequence of the CD which was supposed to be amplified before CDO. Below is a summary of the correspondence between each locus name and which whale species the genomic library used from: Mago: Magondo, Isi: Dolphin, Amz:
Amazon River Dolphin, Tuti: Whale, Sp & Sperm:
Sperm Whale, Bando: Banded Dolphin, and Hump: Humpback Whale. The PCR primers used to isolate the loci suggesting phylogenetic relationships in the order Whales are shown in Table 3 below.

[Table 3] Shown in.

Example 2-2: Supporting monophyly of Whale order
The locus locus Bando1 bottlenose dolphins, to isolate the gene locus Sp316 from a genomic library of the sperm whale. Nucleotide sequence determination of these loci, design of primers in the SINE vicinity region, PCR using genomic DNA of the Artiodactyla as a template
Reaction and separation by agarose gel electrophoresis were performed.
As a result, as shown in FIG. 19, a band pattern in which SINE was commonly inserted in whale eyes was confirmed at the loci Bando1 and Sp316. Since the hippopotamus used as the most closely related outer group does not have this insertion, it is considered that the insertion occurred at the time when the Whale eye was a common ancestor. These loci strongly support the monophyly of Whale order. Locus Bando1
It is considered probable that CD was first inserted in the common ancestor of the whale eye, and then CDO was inserted in the common ancestors of two species of river dolphins, Amazon river dolphin and Laplata river dolphin, in South America. Thus, this locus suggests not only monophyly of the whale order but also that of the South American river dolphin. At the Sp316 locus, CHR-1 type III has been shown to be inserted in a common ancestor of the order Whale. The loci Ba are shown in FIGS.
The results of the ndo1 alignment and the results of the alignment of the Sp316 locus are shown in FIG.

Example 2-3: Supporting monophyly of the dolphin superfamily
Owned locus At the locus Mago19, a CD that is specific to four dolphin superfamily species (Dolphinidae: Collembola, Banded dolphin; Mussel dolphin: Dall's porpoise; Narwhal family: Narwhal)
Insertion of O was confirmed (see FIG. 24). This locus strongly supports the monophyly of the dolphin superfamily. 25 and 2
6 shows the result of alignment of the locus Mago19.

Example 2-4: Infraorder: Delphinida
Loci supporting loci Mago24, Mago26, Mago32, Isi14, Isi36, Isi3
In 8, the insertion of CDO was confirmed only in 4 species of dolphin superfamily, 2 species of South American river dolphin, Amazon river dolphin, La Plata river dolphin, and White dolphin dolphin (see FIGS. 27 and 28).
Thus, these loci support the monophyly of this group. Muizon (1988, 1994) summarized these groups as Delphinida subclasses (classically lower than suborders) based on their morphological characteristics, and is in good agreement with this idea. This is also strongly supported by the analysis of the mitochondrial Cytb gene sequence by Arnason (1996).
It also suggests that the dolphins are multi-systemic and that the Ganges dolphins are more primitive than the other dolphins.
FIG. 29 shows the result of the alignment of the locus Ishi14. 30 to 31 show the results of the alignment of the locus Ishi36. The locus Ish is shown in FIGS.
The result of the i38 alignment is shown. 34 to 35 show the results of alignment of the locus Mago24.
36 to 37 show the results of alignment of the locus Mago26. 38 to 39 show the results of alignment of the locus Mago32.

Example 2-5: Phylogenetic relationship of Red Whale family
At the loci Mago8 and Mago13, which suggest a relationship, the insertion of CDOs specific to Delphinida and two species of blue whales belonging to the family Whale, the Great Whale, was confirmed (see FIG. 40). From this, it can be said that the red whale family is the most closely related taxa to Delphinida in modern species. Also, it can be seen that the scorpionfish is a more primitive group than the red whale. FIG. 41 shows the result of alignment of the locus Mago8. Figure 42 shows the locus Mago1.
The result of the alignment of 3 is shown.

Examples 2-6: Gandhiskawa dolphin family
Locus that suggests a relationship. At the loci Mago21 and Mago22, insertion of CDO specific to Delphinida, red whale, and scorpion dolphin was confirmed (see FIG. 43). Therefore, the stag dolphin family (Indus dolphins are not included in the analysis because there is no sample, but probably form a monophyletic group with swan dolphins.) It became clear.
FIG. 44 shows the result of alignment of the locus Mago21. 45 to 47 show the results of alignment of the locus Mago22.

Examples 2-7: Supporting monophyly of whales
At the loci Sperm8, Sperm28, and Sperm47, the insertion of CD was confirmed in all species of the subfamily Whale, including sperm whales (see FIG. 48). The results of analysis of these loci strongly support the monophyly of the whale, which has been questioned by molecular statistical studies. The loci Sp is shown in FIGS.
The result of the alignment of erm8 is shown. FIG. 51 shows the result of alignment of the locus Sperm28. FIG. 52 shows the result of the alignment of the locus Sperm47.

Examples 2-8: Monophyly of South American river dolphins
In the loci Amz13 and Bando1, which support the locus , insertion of CDO was commonly found in two species of South American river dolphin, Amazon river dolphin and Laplata river dolphin (see FIG. 53). There are some hypotheses about the phylogenetic relationship between the two South American river dolphins and the three species of the northern dolphin, but there is also a theory that the northern dolphin forms a monophyletic group with any of the southern dolphins (Kasuya, 1973; Barnes et al. al., 1985) These two loci strongly supported that the South American river dolphins are monophyletic. Among the several hypotheses proposed so far, it is considered to be the most ideal biogeographical phylogenetic relationship. FIG. 54 shows the result of alignment of the locus Amz13.

Example 2-9: Specific for Amazon River Dolphin
Typical locus Amz11 Fig. 55 shows the band pattern of the locus Amz11 showing that CDO was specifically inserted in Amazon river dolphin. FIG. 56 shows the result of alignment of the locus Amz11.

Examples 2-10: Unique to Red Whale Family
Shows a specific locus Tuti24, Tuti35 band pattern locus Tuti24, Tuti35 indicating that there was a specific insertion of CDO to beaked whale in Figure 57. FIG. 58 shows the result of alignment of the gene locus Tuti24. The gene locus Tuti3 is shown in FIGS.
The results of 5 alignments are shown.

Example 2-11: Three sperm whales
Specific locus for sp9 and sperm whale family
Sp2 Fig. 61 shows the locus Sp9 indicating that CDO was inserted in three sperm whale superfamily species, and the band pattern of sperm whale specific Sp2. 62 to 63 show the results of the alignment of the locus Sp2. FIG. 64 shows the result of the alignment of the locus Sp9.

Examples 2-12: Fin whales (or whiskers)
Hump20, Hump203 locus specific to whale) Figure 65 shows the band patterns of the Hump20 and Hump203 loci indicating that there was CD insertion specific to the fin whale (or whiskers). The locus Hump2 is shown in FIGS.
The result of 0 alignment is shown. Fig. 69 shows the locus H
The result of the alignment of ump203 is shown.

Example 3: Preparation of phylogenetic tree inside Whale Order The SINE insertion patterns obtained from the above results are summarized in a matrix (see FIG. 70). The phylogenetic tree in the whale order estimated based on this is shown in FIG. 71. As much as possible, the nucleotide sequences of the PCR products obtained during the analysis of each locus were determined and aligned. It was confirmed that the resulting band pattern was not caused by secondary insertion or deletion. We also consider that these sequences are important for future research as information on the nuclear gene sequences of Cetacea.

Results The phylogenetic relationships of the modern dental whales revealed by the above examples are summarized below. 1. The whales form a monophyletic group. The sperm whale superfamily is the first divergent group in modern tooth whales. 2. A striated dolphin (probably an Indus dolphin and a single strain) diverged next to sperm whales. It is derived from a different lineage than other river dolphins. 3. Next, a group of red whales diverges. In addition, the genus Beradius and the genus Mesoplodon of the red whale family are single strains. 4. Two species of river dolphins in South America, Amazon river dolphin and La Plata river dolphin, are single strains. 5. The dolphin family, the porpoise family and the narwhal family, which belong to the dolphin superfamily, form a monophyletic group.

The above phylogenetic relationships are not only important phylogenetically, but when a specimen consisting of several animals is provided, it is possible to select an appropriate locus into which SINE has been inserted. It is the basis of the reliability of the species discriminating method using the SINE method according to the present invention, which can discriminate which species the specimen is without the nucleotide sequence analysis.
By establishing a phylogenetic tree by the SINE method for animals other than Whale eyes, it is possible to establish the same species discriminating method in those eyes as in the Whale eyes. This is because the phylogenetic analysis using the molecular statistical method has limitations as described below.
In the past, molecular statistical studies on the phylogenetic relationships of sperm whales have yielded few results suggesting monophyly of the whale. Since these analyzes mainly used the sequences of mitochondrial genes, it was thought that the phenomenon was initially specific to mitochondria, but the analysis of the amino acid sequence of myoglobin (Czelusniak et al.,
(1990) showed the same results, and analysis of nuclear gene IRBP (S
Statistical analysis of the molecular sequence was also shown by mith et al., 1996), in which the polymorphism of the whale (the sperm whale is more closely related to the whale) is statistically advantageous. Then, it seems that the result seems to be that the whale has multiple strains (see Fig. 72). However, the above results clearly suggest that the whales form a monophyletic group, and the monophyly of the whales is almost certain from the viewpoint of morphological characteristics.

Then, why is the result of a molecular statistical analysis of whales such that a sperm whale is closely related to a whale? Outgroup selection is often a problem. In other words, the molecular information only shows the molecular distance of each group, and the roots of the phylogenetic tree (Root, ) Need to be fixed. However, its position may vary depending on the type and number of outgroups,
The analysis that uses only one bovine as the outgroup, such as the early analysis of Arnason (1994), is considered to be quite unreliable. In fact, regarding the Cytb gene, Adachi and Hasegawa (199
According to the research in 5), it is known that changing the outgroup greatly changes the result. However, the subsequent analysis using a large number of Artiodactyla and other ungulates in the outgroup (Arnason et al., 1996) could not suggest monophyly of the whale, so it was simply outgroup. It is unlikely that the choice of was a problem. Next, it is possible that the molecular evolution rate may differ between each taxon of whales. A study by Martin (1993) and others showed a correlation between the body size of an organism and the effects of metabolism, which are thought to affect the rate of base substitution. Generally, the larger the body size, the slower the base substitution rate. I suggested that. In other words, it is considered that small toothed whales such as dolphins caused a statistical error because their molecular evolution rate was higher than that of large bearded whales such as sperm whales and fin whales. However, the maximum likelihood method (M
aximum likelihood analysis) is the maximum savings method (Maximum p
Unlike the arsimony analysis), the results did not change even though the molecular evolution rate among the taxa was different, although the characteristic was that it rarely produced a wrong phylogenetic tree.

It is also quite possible that the species divergence occurred in a short time. For example, if the sperm whales branch off from the whales in a very short time after the branching of the whales, the whales are enough to accumulate common base substitutions among the whales. If there is no time, and if the sperm whales have their own history for a long time until then, the base substitutions that have accumulated during that time may have accumulated during the period of common ancestry of the whales. It is considered that base substitution is unnecessarily performed. Actually, about 35 million years ago, the formation of the Antarctic Ocean Current caused by the northern polarization of the Australian continent is expected to cause speciation due to the adaptive emission of whales at a considerable speed (F
ordyce, 1992). In such cases, it is considered impossible to estimate the lineage by molecular statistical analysis using any analysis method. Also, if a more probable systematic estimation could be made on the assumption of various statistical assumptions, it would be a very artificial systematic tree. This seems to deviate from the idea of "objectivity", which is superior to morphological research.

[0085]

EFFECT OF THE INVENTION One of the advantageous effects of the method for discriminating animal species according to the present invention is that the sequencing step can be omitted.
In general, a sequencer costs at least 15 million yen, and for consumables, the cost of a single sequence is approximately 1200 yen. Therefore, the analysis of a large number of specimens is very expensive. Furthermore, in the case of conducting a commercial analysis by an inspection company, at present one sample is charged at 10,000 yen or more. In terms of time, the sequencing step requires at least about 1 hour and 30 minutes for each sample, and thus the species determination method using the SINE method, which can omit this step, has high analysis efficiency. Most importantly, the analysis of DNA nucleotide sequences involves statistical manipulation. As described above, such a statistical method has a problem that the results may vary depending on the analysis method. On the other hand, in the SINE method, since the species discrimination is performed using the irreversible phenomenon of SINE insertion as an index, the result is clear and no special statistical processing is required. In conclusion, the species discrimination method using the SINE method according to the present invention is
Rather than the species discrimination method based on the comparison of base sequences of the prior art,
It can be said that it is excellent in terms of money, time, and reliability.

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[0087]

[Sequence list]                             SEQUENCE LISTING <110> Okada, Norihiro <120> Method of the identification of a certain species by a SINE metho d <130> 1013131 <140> JP 2000-999,999 <141> 2001-03-30 <160> 325 <170> PatentIn version 3.0 <210> 1 <211> 321 <212> DNA <213> Consensus Sequence. <220> <221> misc_feature <223> CHR-2 SINE General cons. <400> 1 gggcttccct ggtggcgcag tggttaagaa tccgcctgcc aatgcagggg acacgggttc 60 gagccctggt ccgggaagat cccacatgcc gcggagcaac taagcccgtg cgccacaact 120 actgagcctg cgctctagag cccgcgagcc acaactactg agcccacgtg ccacaactac 180 tgaagcccgc gtgcctagag cccgtgctcc gcaacaagag aagccaccgc aatgagaagc 240 ccgtgcaccg caacgaagag tagcccccgc tcaccgcaac tagagaaagc ctgcgcgcag 300 caacgaagac ccaacgcagc c 321 <210> 2 <211> 321 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE FL cons. <400> 2 gggcttccct ggtggcgcag tggttaagaa tccgcctgcc aatgcagggg acacgggttc 60 gagccctggt ccaggaagat cccacatgcc gcggagcaac taagcccgtg cgccacaact 120 actgagcctg cgctctagag cccgcgagcc acaactactg agcccacgtg ccacaactac 180 tgaagcccgc gcgcctagag cccgtgctcc gcaacaagag aagccaccgc aatgagaagc 240 ccgtgcaccg caacgaagag tagcccccgc tcgccgcaac tagagaaagc ccgcgcgcag 300 caacgaagac ccaacgcagc c 321 <210> 3 <211> 300 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE MDI cons. <400> 3 gggcttccct ggtggcgcag tggttaagaa tccgcctgcc aatgcagggg acacgggttc 60 gakccctggt ccgggaagat cccacatgcc gcggagcaac taagcccgtg cgccacaact 120 actgagcctg tgctctagag cccrsgagcc acaactactg aagcccacgt gcctagagcc 180 cgtgctccgc aacaagagaa gccaccgcaa tgagaagccc gcgcaccgca acgaagagta 240 gcccccgctc accgcaacta gagaaagccc tgcgcacagc aacgaagacc caacrcagcc 300 <210> 4 <211> 285 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE MDII cons. <400> 4 gggcttccct ggtggcgcag tggttgagaa tccgcctgcc aatgcagggg acacgggttc 60 gagccctggt ccgggaagat cccacatgcc gcggagcaac taagcccgtg cgccacaact 120 actgagcctg cgtgcygcaa ctactgaagc ccgcgtgcct agagcccgtg ctccgcaaca 180 agagaagcca ccgcgatgag aagcccgtgc accrcaacga agagtagccc ccgatcaccg 240 caactagaga aagcccgcgc gcagcaacga agacccaacg cagcc 285 <210> 5 <211> 261 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE DT cons. <400> 5 ggacttcycy ggtggcgcag tggttaagaa tctgctgcca atgcagggga cacgggttyg 60 agccctggtc tgggaagatc ccacatgccg tggagcract aagcccatgc gccacaacta 120 ctgagcctgt gtgcctagag cccgtgctct gcaacaagag aagccaccac aataagaagc 180 ccgtgcaccg caacgaagag tagcccccac tcaccgcaac tagagaaagc ctgcgtgcag 240 caacgaagac ccaacacagc c 261 <210> 6 <211> 262 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE CD cons. <400> 6 gggcttccct ggtggcgcag tggttgagaa tctgcctgcc aatgcagggg acacgggttc 60 gagccctggt ctgggaagat cccacatgcc gcggagcaac taggcccgtg agccacaact 120 actgagcctg cgcgtctgga gcctgtgctc cgcaacaaga gaggccgcga tagtgagagg 180 cccgcgcacc gcgatgaaga gtggcccccg cttgccgcaa ctagagaaag ccctcgcaca 240 gaaacgaaga cccaacacag cc 262 <210> 7 <211> 194 <212> DNA <213> Consensus Sequence <220> <221> misc_feature <223> CHR-2 SINE CDO cons. <400> 7 gggcttccct ggtggcgcag tggttgagag tccgcctgcc gatgcagggg acacgggttc 60 gtgccccggt ccgggaagat cccacatgcc gcggagcggc tgggcccgtg agccatggcc 120 gctgagcctg tgcgtccgga gcctgtgctc cgcaacggga gaggccacaa cagtgagagg 180 cccgcgtacc gcaa 194 <210> 8 <211> 641 <212> DNA <213> Bando 1 Bottlenosed <400> 8 ttgtcaaggt gcttcgcttt agcgattcta ataggtatgt agtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatattga ggatcttctc atatgctttt ttaccatctg 120 tgtaccttct ttggtgaagt gtttctcaaa tcttttgcac atttaagaaa ttggattttg 180 ggacttccct ggtggtgcag tggttgagaa tccacctgcc agtgcagagg acatgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccgtg tgccacaact 300 accgagcctg tactctagag cccacgagcc acaactactg aagcccgtgc acctagagcc 360 catgctccac aacaagagta gccaccatga tgagaagccc acgcacctca atgacaagtg 420 gtccccgctc accacaacta gagaaagccc acgtgcagca acgaagaccc aacgcagcca 480 aaaattaatt aattaattga aaaaaattgg atagtgtgct tttggtgata ttcttataat 540 ttagagatta attcacttta agttgattct cctttaaaat ttaaatatcc cagtataaaa 600 attaataaac agaagtctct attaaaacag agtctggggg c 641 <210> 9 <211> 644 <212> DNA <213> Bando 1 Short-finned <400> 9 ttgtcaaggt gcttcgcttt agcgattcta ataggtatgt agtagatatc tcatcatggt 60 tttaatgcaa ttctctaatg actaatatta gaggatcttc tcatatgctt ttttaccatc 120 tgtgtacctt ctttggtgaa gtgtttctta aatcttttgc acatttaaga aattggattt 180 tgggacttcc ctggtggtgc agtggttgag aatccacctg ccagtgcagg ggacatgggt 240 tcgatccctg gtccaggaag atcccacatg ccacagagca actaagcccg tgtgccacaa 300 ctaccgagcc tgtactctag agcccacgag ccacaactac tgaagcccgt gcacctagag 360 cccatgctcc acaacaagag aagccaccat gatgagaagc ccacgcacct caatgtcaag 420 tggtccccgc tcaccacaac tagagaaagc ccacgtgcag caacgaagac ccaacgcagc 480 caaaaataaa ttaattaatt gaaaaaaaat tggaaagtgt gcttttggtg aaattcttat 540 aatttagaga ttaattcact ttaagttgat tctcctttaa aatttaaata tcccagtata 600 aaaattaata aacagaagcc tctattaaaa cagagtctgg gggc 644 <210> 10 <211> 637 <212> DNA <213> Bando 1 Dall's <400> 10 ttgtcaaggt gcttcgcttt agcgattcta ataggtatgt agtgatatct catcatggtt 60 ttaatgcaat tctctaataa ctaatattga ggatcttctc atatgctttt ttaccatctg 120 tgtaccttct ttggtgaagt gtttctcaaa tcttttgcac atttaagaaa ttggattttg 180 ggacttccct ggtggcacag tggttgagaa tccacctgcc aatgcagggg acatgggttc 240 gatccctggt ccgggaagat cccacatgac acagagcaac taagcccgtg tgccacaact 300 accgagcctg tgctctagag cccacgagcc acaactactg aagcccatgc acctagagcc 360 catgctccac acaagagaag ccaccatgat gagaagccca cgcacctcaa tgacaagtgg 420 tccccactca ccacaactag agaaagccca cgtgcagcaa cgaagaccca acacagccaa 480 aaataaatta attgaaaaaa aattggatag tgtgcttttg gtgatattct tataatttag 540 agattaattc actttaagtt gattctcctt taaaatttaa atatcccagt ataaaaatta 600 ataaacagaa tcctctatta aaacagagtc tgggggc 637 <210> 11 <211> 639 <212> DNA <213> Bando 1 Narwhal <400> 11 ttgtcaaggt gcttcgcttt agcgattcta ataggtatgt agtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatattga ggatcttctc atatgctttt ttaccatctg 120 tgtaccttct ttggtgaagt gtttctcaaa tcttttgcac atttaagaaa ttggattttg 180 ggacttccct ggtggtgcag tggttgagaa tccacctgcc aatgcagggg acatgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccgtg tgccacaact 300 accgagcctg tgctctagag cccacgagcc acaactactg aagcccgtgc acctagagcc 360 catgctccac aacaagagaa gccaccatga tgagaagccc acgcacctca atgacacgtg 420 gtccccgctc accacaacta gagaaagccc acgtgcagca acgaagaccc aacgcagcca 480 aaaataaatt aattgaaaaa aaattggata gtgtgctttt ggtgatgttc ttataattta 540 gagattaatt cactttaagt tgattctcct ttaaaattta aatatcccag tataaaaatt 600 aataaaacag aagcctctat taaaacagag tctgggggc 639 <210> 12 <211> 861 <212> DNA <213> Bando 1 Amazon <220> <221> CDO <222> (536) .. (755) <400> 12 ttgtcaaggt gcttcgcttt agcaattcta ataggtgtgt agtgatatct catcatggtt 60 ttaatgcaat tctttaatga ctaatattga ggatcttctc atatgctttt ttaccatctg 120 cgcaccttat ttggtgaagt gtttctcaaa tcttctgcac atttaaaaaa ttggatttta 180 ggacttccct ggtggtgcag tggttgagaa tctgcctgtc aatgcagggg acatgggttc 240 aatccctggt ccaggaagat cccacatgcc acagagcaac taagcacgtg tgccacaact 300 accgagcctg tgctctagag cccatgagcc acaactactg aagcccgtgc acctagagcc 360 catgctccaa aacaagagaa gccacaatga tgagaagccc acgtacctca atgacgagtg 420 gcctccgctc gccacaacta gagaaagctc acgtgcagca acgaagaccc aacgcagcca 480 aaaataaatt aattaatttt aaaaaaatgg atggtgtgct tttggtgata ttcttttttt 540 ttttttcttt cggtacacgg gcctctcacc gttgtggtct ctcccgttgc tgagcacagg 600 ctctggacgt gcaggcccag cggccatggc tcacgggccc agccgccccg cggcatgtgg 660 gatcttcccg gaccggggca cgaacccgtg tcccctgcat cggcaggcgg acttccaacc 720 actgcgccac cagggaagcc cttggtgata ttcttataat ttagagatta attcacttta 780 agttgattct cctttaaaat ttaaatatcc cagtataaaa attaataaac agaagcctct 840 attaaaacag agtctggggg c 861 <210> 13 <211> 868 <212> DNA <213> Bando 1 La Plata <220> <221> CDO <222> (537) .. (762) <400> 13 ttgtcaaggt gcttcgcttt agcaattcta ataggggtgt agtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatattga ggatcttctc atatgctttt ttaccatctg 120 cgtaccttat ttggtgaagt gtttctcaaa tcttctgcac atttaaaaaa ttggatttta 180 gaatttccct ggtggtgcag tggttgaaaa tccgcttgcc aatgcaggga acatgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccgtg tgccacaact 300 accgagcctg tgctctagag cccatgagcc acaactactg aagcctgtgc acctagagcc 360 catgctccac aacaagagaa gccaccagga tgtgaagccc acgcacctca atgatgagtg 420 gccactgctc gccacaacta gagaaagccc atgtgcagca acgaagaccc aatgcagcca 480 aagataaatt aattaattaa aaaaaaattg gatggtatgc ttttggtgat attctttttt 540 tttttttttt ttcccccctg tatgtgggcc tctcaccgtt gtggcctctc ccgttgtgga 600 ggacaggctc tggacgcaca ggcccagcag ccatggctca cgggcccagc cgctccacag 660 catgtgggat cctcccggac cggggcacga acccgtgtcc cctgcatcgg caggaggact 720 ccccaccact gcgccaccag ggaagccctt ggtgatattc ttataattta gagattaatt 780 cactttaagt tgattctcct ttaaaattta aatatcacag tataaaaatt aataaacaga 840 agcctctatt aaaacagagt ttgggggc 868 <210> 14 <211> 640 <212> DNA <213> Bando 1 Baiji <400> 14 ttgtcaaggt gcttcgcttt agcaattcta ataggtgtgt agtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatattga ggatcttctc atatgccttt ttaccatctg 120 cgtaccttat ttggtgaagt gtttctcaaa tcttctgcac atttaaaaaa ttggatttta 180 ggacttccct ggtggtgcag tggttgagaa tccgcctgcc aatgcagggg acacgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccgtg tgccacacct 300 accgagcctg tgctctagag cccatgagcc acaactactg aagcccgtgc acctaaagcc 360 catgctccac aacaagagaa gccaccataa tgagaagccc acgcacctca atgacgagtg 420 gcccccactc gccacaacga gaggaagccc acatgcagga acgaagaccc aacgcagcca 480 aaaatcaatt aattaattta aaaaattgga tagtgtgatc ttggtgatag tcttataatt 540 tagagattaa ttcactttaa gttgattctc ctttaaaatt taaataaccc agtataaaaa 600 ttaataaaca gaagcctcta ttaaaacaga gtctgggggc 640 <210> 15 <211> 627 <212> DNA <213> Bando 1 Beaked <400> 15 ttgtcaaggt gcttcgcttt agtgagtcta ataggtgtgt agtgatatct catcatggtt 60 ttaattctct aatgactaat attgaggatc ttctcatatg cttttttacc atctgcatac 120 cttctttggt gaagtgtttc tcaaatcttt tgcacattta aaaattggat tttgggactt 180 ccctggtggt gcagtggttg agaatctgcc tgccaatgca ggggacacgg gtttgatccc 240 tggtccagga agatcccaca tgccacagag cagctaagcc catgtgccac aactaccgag 300 cctgtgctct agagcccaca agccacaact actgaagccc gtgcacctag agcccatgct 360 ccacaacaag agaagccacc atgatgagaa gcccatgcac ctcaatgatg agtagccccc 420 gcttgccaca actagagaaa gcccacgtgc agcaaagacc caacacagcc aaaaataaat 480 taattttaaa aaattggata gtgtgctttt ggtgatattt ttataattta gagattaatt 540 cactttaagt tgattctcct ttaaaattta aatatcccag ctaaaaatta ataaacagaa 600 gcctctatta aaacagagtc tgggggc 627 <210> 16 <211> 680 <212> DNA <213> Bando 1 Ganges <400> 16 ttgtcaaggt gcttcgcttt agcgattcta ataggtgtgt agtgatatct tgtcatggtt 60 ttaatgcaat tctctaatga ctaatactaa ggatcttctc atatgctttt ttaccatctg 120 tgtaccttct ttggtgaagt gtttctcaaa tcttttgcac atttaaaaat ttggattttg 180 ggacttccct ggtggtgcag tggttgagaa tctgcctacc aatgcagggg acatgggttc 240 gagccctggt ccaggaagat cccacatgcc acagagcaac taagccggtg tgccacaact 300 accgagcctg tgctctagag cccacgagcc acaactactg aagcctgtgc acctagagcc 360 catgctccac aacaagagaa gccaccatga tgagaagccc acgcacctca atgaagagta 420 gccctcgctc gccacaacta gagaaagcac acatgcagca acgaagaccc cctgctcgcc 480 acaactagag aaagcccacg tgcagcaacg aagacccaac gcagccaaaa ataaattgat 540 ttaaaaaaaa ttggatagtc tgcttttggt gatattctta taatttagag attcacttta 600 agttgattct cctttaaaat ttaaatatcc cagtataaaa attaataaac agaagcctct 660 attaaacaga gtctgggggc 680 <210> 17 <211> 657 <212> DNA <213> Bando 1 Sperm <400> 17 ttgtcaaggt gcttcgcttt agcgattcta ataggtgtgt agtgatatgt catcatggtt 60 ttaatgcaat tctctaatga ctaatattga ggatcttctc atatgctttt tttttttttt 120 tttttttttt ttaccatctg cgtaccttct ttggtgaagc gtttctcaaa tcttttgcac 180 atttaaaaaa ttggatctgg ggacttccct ggtggcacag tggttgagaa tccgcctgcc 240 aatgcagggg acacgagttc gatccctggt ccaggaagat cccacaggcc acagagcaac 300 taagcccgtg tgccacaacg actgagcctg tgctctagag cccatgagcc acaactactg 360 aagaccgtgc acctagagcc catgctccac aacaagagaa gccaccgtga tgagaagccc 420 acgcacgtca atgaagagca gcccccgctc gccacaacta gaaaaagccc acgtgcagca 480 acgaagaacc aatgcagcca aaaataaatt aattaaaaaa aattggatag tgtgcttttg 540 gtgatattct tataatttag agattaattc actttaagtt gattctcctc taaaatttaa 600 atatcccagt ataaaaatta ataaacagaa gcctctatta aaacagagtc tgggggc 657 <210> 18 <211> 684 <212> DNA <213> Bando 1 Humpback <400> 18 ttgtcaaggt gcttcgcttt aggatttcta ataggtgtgt ggtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatgttga ggatcttctc gtatgttttt ttaccatctg 120 cgtaccttct ttggtgaagt gtttctcaaa acttttgcac atttaaaaaa ttggattgtg 180 ggatttccct ggtggtgcag tggttgagaa ttcgcctgcc aatgcagggg acatgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccgtg tgccacaact 300 accgagcctg tgctctagag cccatgagcc acaactactg aagcccgtgc acctaaagcc 360 catgctccac agcaagagaa gccaccatga tgagaagccc acgcacctca atgaagagta 420 gcccccgctc gccacaacta gagaaagccc acgtgcagca gtgaagaccc cccgcttacc 480 acaactagag aaagcccatg tgcagcaacg aagacccaac gcagccaaaa ataaattaac 540 taaaaaaaat tggattgtgt gcttttggtg atattcttat aatttagaga ttaattcatt 600 ttaagttgat tctcctttaa aatttaaata tcccagtata aaaattaata aacagaagcc 660 tctattaaaa cagagtctgg gggc 684 <210> 19 <211> 685 <212> DNA <213> Bando 1 Fin <400> 19 ttgtcaaggt gcttcgcttt agcgattcta ataggtgtgt ggtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatgttga ggatcttctc gtatgctttt ttaccatctg 120 cgtaccttct ttggtgaagt gtttctcaaa acttttgcac atttaaaaaa ttggattgtg 180 ggatttccct ggtggtgcag gggttgagaa tttgcctgcc aatgcagggg acatgggttc 240 gatccctggt ccaggaagat cccacatgcc acagagcaac taagcccatg tgccacaact 300 accgaacctg tgctctagag cccatgagcc acaactactg aagcccgtgc acctaaagcc 360 catgctccac agcaagagaa gccaccatga tgagaagccc acacacctca atgaagagta 420 gcccccgctc gccacaacta gagaaagccc acgtgcagca gtgaagatcc cccacttacc 480 acaactagag aaagcccatg tgcagcaacg aagacccaac gcagccaaaa ataaattaat 540 taaaaaaatt ggattgtgcg cttttggtga tattcttata atttagaaat taattcattt 600 taagttgatt ctcctttaaa atttaaatat ccctagtatc taaaattaat aaacagaagc 660 ctctattaaa acagagtctg ggggc 685 <210> 20 <211> 683 <212> DNA <213> Bando 1 Minke <400> 20 ttgtcaaggt gcttcgcttt agcgattcta ataggtgtgt ggtgatatct catcatggtt 60 ttaatgcaat tctctaatga ctaatgttga ggatcttctc gtatgctttt ttaccatctg 120 cgtaccttct ttggtgaagt gtttctcaaa acttttgcac atttaaaaaa ttggattgtg 180 ggatttccct ggtggtgcag tggttgagaa ttcgcctgcc aatgcagggg acatgggttc 240 gatccctggt ccaggaagat ccacatgcca cagagcaact aagcccgtgt gccacaacta 300 ccgagcctgt gctctagagc ccatgagcca caactactga agcccgtgca cctaaagccc 360 atgctccaca gcaagagaag ccaccatgat gagaaaccca cgcacctcaa tgaagagtag 420 cccccgctcg ccacaactag agaaagccca cgtgcagcag tgaagacccc ctgcttacca 480 caactagaga aagcccatgt gcagcaacga agacccaacg cagccaaaaa taaattaatt 540 aaaaaaaatt ggattgtgtg cttttggtga tattcttata atttagagat taattcattt 600 taagttgatt ctcctttaaa atttaaatat cccagcataa aaattaataa acagaagcct 660 ctattaaaac agagtctggg ggc 683 <210> 21 <211> 303 <212> DNA <213> Bando 1 Hippo <400> 21 ttgtcaaggt gcttgcttta gtgattctaa taggtgagta gtgatatctc accatggttt 60 taatacaatt ctctaacaac taatactgag gatcctctca tatacttatt taccatctgt 120 gtatcttctt cggtgaaatg tttttcaaat cttttgcaca tttaaaaatt agattgtgtgg 180 cttttggtga tattcttata atttagagat taattcattt taagttgatt cttctttaaa 240 atttataaat cccagtataa aaattaataa acagaaacct ctattaaaac agagtctggg 300 ggc 303 <210> 22 <211> 365 <212> DNA <213> Sp316 Bottlenosed <400> 22 agtgtggctt catatccctt ggcaattctc agcagcaatg agacagagta ctcaggatgc 60 cttctcgcat actcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gacaagctac ataatcggga cttccctggt ggcccagtgg ctaagtctcc 180 gcactcccaa tgcagggggc ctgggttcga tccctggtca gggaactaga tcccatatgc 240 atgctgcaac taagagccag tgcagacaaa taaataagta aataaaacat caaaaaaaaa 300 aacaccccat aattaaccac aaaattaact catatcataa aggtctgaat ttgactaaac 360 cccat 365 <210> 23 <211> 364 <212> DNA <213> Sp316 Short-finned <400> 23 agtgtggctt catatccctt ggcaattctc agcagcaatg agacagagta ctcaggatgc 60 cttctcgcat actcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gacaaactac ataattggga cttccctggt ggcccagtgg ctaattctcc 180 gcactcccaa tgcaaggggc ctgggttcga tccctggtca gggaactaga tcccatatgc 240 atgctgcaac taagagccag tgcagacaaa taaataagta aataaaacat caaaaaaaaa 300 acaccccaca attaaccacg aaattaactc atatcataaa gggctgaatt tgactaaacc 360 ccat 364 <210> 24 <211> 362 <212> DNA <213> Sp316 Dall's <400> 24 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac ataattggga cctccctggt ggcccagtgg ctaagtctct 180 gcactcccaa tgcagtgggc ccgggttcga tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagagccag tgcacacaaa taaataagta aataaaacat caaaaaaaaa 300 aacccataat taaccacaaa attaactcat atcataaagg tctgaatttg actaaacccc 360 at 362 <210> 25 <211> 364 <212> DNA <213> SP316 Narwhal <400> 25 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagcgta ctcaggatgc 60 cttcttgcat attcatacaa aaacctaatt aagatgacaa taaaatagct ttagttggac 120 aaatgagcaa gtaaaaacta cataattggg acttccctgg tggcccagtg gctaagtctc 180 tgcactccca atgcaggggg cccgggttcg atccctggtc agggaactag atcccacatg 240 catgctgcaa ctaagagcca gtgcacacaa ataaataagt aaataaaaca tcaaaaaaaa 300 aaccccataa ttaaccacaa aattaactca tatcataaag gtctgaattt gactaaaccc 360 catg 364 <210> 26 <211> 363 <212> DNA <213> Sp316 Amazon <400> 26 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac ataattggga cttccctcgt ggcccagtgg ctaagtctcc 180 gcactcccaa tgcagggggc ccgggttcga tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagagccag tgcagacaaa taaataagta aataaaacat caaaaaaaac 300 ccacccataa ttagtcacaa aattaactca tatcctaaag gtctgaattt gactaaaccc 360 cat 363 <210> 27 <211> 363 <212> DNA <213> Sp316 La Plata <400> 27 agtgtggctt catacccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcgtacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactat ataactggga cttccctggt ggcccagtgg ctaagtcgct 180 gcactcccaa tgcagggggc ccgggttcga tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagttccag tacagacaaa taaataagta aataaaacat aaaaaaaaaa 300 ccaaaaacca taattagcca caaaattaac tcatatccta aaggtctgaa tttgactaaa 360 ccc 363 <210> 28 <211> 362 <212> DNA <213> Sp316 Baiji <400> 28 agtgtggctt catatccctt ggcaattctc aatagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac ataattggga cttccctggt gacccagtgg ctaagtctcc 180 gcactcccaa tgcagggggc ccgggttcga tccctggcca gggaactaga tcccacatgc 240 atgctgcaac taagagccag tgcagacaaa taaataagta aataaaacat aaaaaaaaaa 300 acaccataat taaccacaaa attaactcat atcctaaagg tctgaatttg actaaacccc 360 at 362 <210> 29 <211> 359 <212> DNA <213> SP316 Beaked <400> 29 agtgtggctt catatgcctt ggcaattctc aacagcaatg agacagagta ctcagggtgc 60 cttcttgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac ataattggga cttccctggt ggcccagtgg ctaagtctcc 180 gcactcccaa tgcagggggc ccgggttcga tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagagccgg tgcagacaaa taaataagta aataaaacat caaaaaaaaa 300 accccataat taaccacaaa agtaacttat aacctaaagg tctgaatttg actaaaccc 359 <210> 30 <211> 348 <212> DNA <213> Sp316 Ganges <400> 30 agtgtggctt catatgcctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaaat aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac agaattggga cttccctggt ggcccagtgg ctaagtctcc 180 acactcccaa tgcagggggc ccgggtttga tccatggtca gggaactaga tcccacatgc 240 atgctgcaac taagagccgg tgcagacaaa caaataagta aaataaaaca tcaaaaaaaa 300 aaccccataa ttaaccacaa aagtaactca tatcctaaag gtctgaat 348 <210> 31 <211> 354 <212> DNA <213> SP316 Sperm <400> 31 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacac aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaactac ataattggga cttccctggt ggtccagtgg ctaagtctct 180 gcactcccaa tgcagggggc ccgggttcga tccctggtca ggaaactaga tcccaaatgc 240 atgctgcatc taagagccgg tgcagacaaa taaataagta aataaaacat caaaaaaaac 300 ccccataatt aaccacaaaa gtaactcata tcctaaaggt ctgaatttga ctaa 354 <210> 32 <211> 361 <212> DNA <213> Sp316 Humpback <400> 32 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaaccac ataattggga cttccctggt ggtccagtgg ctaagtctct 180 gcactcccaa tgcagggggc ccaggttcca tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagacccgg tgcagacaaa taaataagta aataaaacat caaaaaaaac 300 cccataatta accacaaaag taactcatat cctaaaggtc tgaatttgac taaaccccat 360 g 361 <210> 33 <211> 360 <212> DNA <213> Sp316 Fin <400> 33 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgt 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaaccac ataattggga cttccctggt ggtccagtgg ctaagtctct 180 gcactcccaa tgcagggggc ccgggttcca tccctggtca gggaactaga tcccacatgc 240 atgctgcaac taagacccgg tgcagacaaa taaataagta aataaaacat caaaaaaaac 300 cccataatta accacaaaag taactcatat cctaaaggtc tgaatttgac taaaccccat 360 <210> 34 <211> 365 <212> DNA <213> Sp316 Minke <400> 34 agtgtggctt catatccctt ggcaattctc aacagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa taaaatagat ttagttggac 120 aaatgagcaa gaaaaaccac ataattggga cttccctggt ggtccagtgg ctaagtctct 180 gcactcccaa tgcagggggc ccgggttcca tccctggtca gggaactaga tcccacatgc 240 atgctgcagc taagacccgg tgcagacaaa taaataagta aataaaacat caaaaaaaaa 300 aaaaacccat aattaaccac aaaagtaact catatcctaa aggtctgaat ttgactaaac 360 cccat 365 <210> 35 <211> 198 <212> DNA <213> Sp316 Hippo <400> 35 agtgtggctt cataaaattt ggcaactctc aatagcaatg agacagagta ctcaggatgc 60 cttctcgcat attcatacaa aaacctaatt aagatgacaa caaaataggt ttagttagac 120 agatgaacaa gaaaaaccat atggttaccc aaaaaagtaa ctcttatcct aaaggtctga 180 atttgactaa accccatg 198 <210> 36 <211> 493 <212> DNA <213> Mago 19 Bottlenosed <220> <221> CDO <222> (139) .. (337) <400> 36 ctgcacagtt ttggctcaat cattacaact gctataggtt gagtggtgtc ccctgaaaag 60 atatgttgaa gtcctagggt tgtgagtgtt attttaaaat gggtctctaa agacataatc 120 attaaagatg aagtcctagg gctttcctgg tggcgcagtg gttgagagtc cgcctgccga 180 tgcaggggac acgggttcat gccccgatcc gggaggccca tgagccatgg ccgctgagcc 240 tgtgtgtccg gagcctgtgt ccgcaacggg agaggccacc acagtgagag gcccacgtac 300 cgcaaaaaaa aaaaaaaaaa aaaaagatga agtcctagag tgggctataa tccaatatgg 360 caggtttcct tattaaaaag agaaaaaaaa agacacagac agatatacac agaggaaaga 420 tgatgtgaag acatacgggg aaaacgtcat gtgatgagga agcagagaat aaagtgatct 480 tacacatgac cag 493 <210> 37 <211> 495 <212> DNA <213> Mago 19 Narwhal <220> <221> CDO <222> (140) .. (338) <400> 37 ctgcacagtt ttggctcaat cattacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctagggc tgtgaatgtt atttgaaaat ggggtctcta cagacataat 120 cattaaagat gaagtcctag ggcttccctg gtggcgcagt ggttgagagt ccgcctgccg 180 atgcagggga cacgggttcg tgccccagtc cgggaggccc gtgagccatg gccgctgagc 240 ctgtgtgtcc ggagcctgtg tccgcaacgg gagaggccac cacagtgaga ggcccacgta 300 ccgcaaaaaa aaaaaagaaa aaaaaagatg aagtcctaga gtgggctata atccaatatg 360 gcaggtttcc ttattaaaaa gagaaaaaaa agacaccgac agatatacac aggggaaaga 420 tgatgtgaag acatacgggg aaaacgtcat gtgatgagga aggcagagaa taaagtgatg 480 cttacacatg accag 495 <210> 38 <211> 498 <212> DNA <213> Mago 19 Dall's <220> <221> CDO <222> (140) .. (341) <400> 38 ctgcacagtt ttggctcaat cattacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctagggc tgtgaatgtt atttgaaaat ggggtctcta cagacataat 120 cattaaagat gaagtcctag ggcttccctg gtggtgcagt ggttgagagt ccgcctgccg 180 atatagggga cacgggttcg tgccccagtc cgggaggccc gtgagccatg gccgctgagc 240 ctgtgtgtcc agagcctgtg tccgcaacgg gagaggccat cacagtgaga ggcccacata 300 ccgcaaaaaa aaaaaaaaaa aaaaaaaaag atgaagtcct agagtgggct ataatccaat 360 atggcaggtt tccttattaa aaagagaaaa aaaagacacc gacagatata cacagaggaa 420 agattatgtg aagacatacg gggaaaacgt catgtgatga ggaaggcaga gaataaagtg 480 atgcttacac atgaccag 498 <210> 39 <211> 307 <212> DNA <213> Mago 19 Amazon <400> 39 ctgcacagtt ttggctcaat cattacagct gcttataggt tgagtggtgt cccctgagaa 60 gatatgttga agccctaacc ctcagtacct gtgaatgtta tttgaaaatg gggtctctac 120 agacataatc attaaagatg aagtcctaga gtgggctgta atccaatatg gcaggtttcc 180 ttattaaaaa gagaaagaaa aaagacacag acagatatac acagagaaaa gatgatgtga 240 aggcatacgg ggaaaacggc atgtgatgag aaaggcagag aataaagtga tgcttacaca 300 tgaccag 307 <210> 40 <211> 309 <212> DNA <213> Mago 19 La Plata <400> 40 ctgcacagtt ttggctcaat cattacagct gcttataggt tgagtggtgt cccctgagaa 60 gatatgttga agtcctaacc ctcagtacct gtgaatgtta tttgaaaatg gggtctctac 120 agacataatc attaaagatg aagtcctaga gtgggctgta atccaatatg gcaggtttct 180 tattaaaaag agaaaaaaaa aaaaagacac agacagatat acacagagga aagatgatgt 240 gaaggcatac gggggaaaca gcatgtgatg agaaaggcag aaaataaagt gatgcttaca 300 catgaccag 309 <210> 41 <211> 293 <212> DNA <213> Mago 19 Baiji <400> 41 ctgcacagtt ttggctcaat cattacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctaaccc tcagtacctg tgaatgttat ttgaaaatgg ggtctctaca 120 gacataatca ttaaagatga agtcctagag tgggctgtaa tccaatatgg caggtttcct 180 tattaaaaag agaaaaaaaa gacacagaca gaggaaagat gatgtgaaga catacgggga 240 aaacatcatg tgatgaggaa ggcagagaat aaagtgatgc ttcacatgac cag 293 <210> 42 <211> 311 <212> DNA <213> Mago 19 Beaked <400> 42 ctgcacagtt ttggctcaat cattacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctaaccc tcagcacctg tgaatgttat ttgaaaatgg agtctctata 120 gacataatca ttaaagatga agtcctagag tgggctgtaa tccaacatgg caggtttctt 180 tattaaaaag agaaagaaaa aagacacaga cagatataca cagaagaaag atgatgtgaa 240 gacatatggg gaaaacacat catcatgtga tgatgaaggc agagaataaa gtgatgctta 300 cacatgacca g 311 <210> 43 <211> 313 <212> DNA <213> Mago 19 Ganges <400> 43 ctgcacagtt ttggctcaat cactacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctaaccc ttagtacctg cgaatgttat ttgaaaatgg ggtctctaca 120 gacataatca ttaaagatga agtcctagag tgggctgtaa cccaatatgg caggtttcct 180 tcttaaaaag agaggaaaaa aaaaaacaaa acacagacag atatacacag aggaaagatg 240 atatgaagac atacggggaa aacgtcatat gatgattaag gcagagaata aagtgatgct 300 tacacatgac cag 313 <210> 44 <211> 304 <212> DNA <213> Mago 19 Sperm <400> 44 ctgcacagtt ttggctcaat cattgcagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa atcccaaccc tcagtacctg tgaacgttat ttgaaaatgg ggtctttaca 120 gacataatca ttaaagatga agtcctagag tgggctgtaa tccaatatgg caggtttcct 180 tattaaaaag agaaaaaaaa gacacagaca gatacacaca gaggaaagat gatgcgaaga 240 catacgggga agacgtcatg tgatgatgaa ggcagagaat aaagtgatgc ttacacatga 300 ccag 304 <210> 45 <211> 308 <212> DNA <213> Mago 19 Humpback <400> 45 ctgcacagtt ttggctcaat cattacaact gctatgagtt gagtggtatc ccctgagaag 60 atatgttgaa gtcctaaccc tcagtacctg tgaatgttat ttgaaaatgg agtctttaca 120 gacataatca ttaaagatga agtcctagag tgggctgtaa tccaatatga cagttttcct 180 tattaaaaag agggaaaaaa aagacacaaa gacagatata cacagaggaa agatgatgtg 240 aagacatacg gagaaaacat catgtgatga tgaaggcaga gaataaagtg atgcttacac 300 atgaccag 308 <210> 46 <211> 305 <212> DNA <213> Mago 19 Minke <400> 46 ctgcacagtt ttggctcaat cattacagct gctataggtt gagtggtgtc ccctgagaag 60 atatgttgaa gtcctaaccc tcagtacctg tgaatgttat ttgaaaatgg ggtctttaca 120 gacataatca ttaaagatga agtcctagag tgggctgtaa tccaatatgg cagggttcct 180 tattaaaaag agaaaaaaag acacaaagac agatatacac agagaaaaga tgatgtgaag 240 acatatggag aaaacatcat gtgatgatga aggcagagaa taaagtgatg cttacacatg 300 accag 305 <210> 47 <211> 594 <212> DNA <213> Ishi 14 Dall's <220> <221> CDO <222> (229) .. (450) <400> 47 ttcccctata ttctccatgg ttttattgta aagaggcatt atacttccaa atgaagaata 60 aaaatattgc aatcactccc caaccctgag ggtgaggttc ctaaaatttt aacattatac 120 ttccaaatga agaataaaaa tattgcaatc actccccaac cctgagggtg aggttcctaa 180 aattttaagt tttcataaat gacaggggaa tttaagaact taaggtaggg gcttccctgg 240 tggmtcagtg gttgagagtt ggcctgccga tgcaggggac acgggtttgt gccccagtct 300 gggaagatcc catatgccgc agagcggctg ggcccgtgag ccataaccgc tgagcctgcg 360 cctccagagc ctgtgctccg caacgggaga ggccacaaca gtgagaggcc cgcgtaccaa 420 aaaaaaaaga aaatgaagaa cttaaggtag ggtgtagaga agaagatgga tattcaccat 480 atgaaaatat ttatcattat aattttatta catacataaa aagaaaaaga gaatagcaga 540 ataaaaagaa agaaaataaa ttgcacccac atatcttaat gagtaataat gacc 594 <210> 48 <211> 518 <212> DNA <213> Ishi 14 Amazon <220> <221> CDO <222> (153) .. (380) <400> 48 ttcccctata ttctccatgg ttttattgta aagaggcatt atacttccaa aagaagaata 60 aaaatattgc aatcactccc caaccctgag ggtgaggttc ctaaaatttt aagttttcat 120 atatgatagg ggaatttaag aacttaagct aggggcttcc ctggtggcgc agtggttgag 180 agtccgcctg ccgatgcagg ggacacgggt tcgtgcccca gtccgggagg atcccatatg 240 ccacagagca gctggacctg tgagccatgg ccactgagtc tgtgcatcca cagcctgtgc 300 tccgcaacgg gagaggccac aacagtgaga ggcctgcgta ccaaaaaaaa aaaaaaaaaa 360 aaaaaaagaa cttaaggtgg ggtgtagagc agaagatggt tattcaccat atgaaaatat 420 ttatcattat aattttatta catacataaa aagaaaaaga atagcagaat aaaaagaaag 480 gaaattgcac ccacatatct taatgggtaa taatgccc 518 <210> 49 <211> 288 <212> DNA <213> Ishi 14 Ganges <400> 49 ttcccctata ttctccatgg ttttattgta aagaggcatt gtagttccaa atgaagaata 60 aaaatattgc aatcactccc caaccctgag ggtgaggttc ctaaaatttt aagtttttgt 120 aactaatagg ggaatttaag aacttaaggt agggtgtaga gaaggagatg gatattcaca 180 atatgaagat atttatcatt attttattac ataaaaagaa aaagagaata gcagaataaa 240 acgctgaaaa taaattgcac ccacatatct taatgagtaa taatgacc 288 <210> 50 <211> 293 <212> DNA <213> Ishi 14 Sperm <400> 50 ttcccctata ttctccatgg ttttattgta aagaggcatt atacttccaa ataaagaata 60 aaaatattgc aatcactccc caaccctgag ggtgaggttc ctaaaatttt aagttttcat 120 aaatgatagg ggaatttaag aacttaaggt agggtgtaaa gaagatggat attcaccata 180 tgaagatatt tatcattata attttattac atacataaaa agaaaaagag agtagcagaa 240 taaaaagaaa gaaaataaat tgcacccaca tatcttaatg agtaataatg acc 293 <210> 51 <211> 293 <212> DNA <213> Ishi 14 Humpback <400> 51 ttcccctata ttctccatgg ttttattgta aagagacatt atacttccaa atgaagaata 60 aaaatattgc aatcactccc cgaccctgag ggtgaggttc ctaaaatttt aagttttcat 120 aaatgatagg ggaacttaag aacttaaggt agggtgtaga gaagaagatg gatattcacc 180 atatgaagat atttatcata attttattac atacataaaa agaaaaagag aatagcagaa 240 taaaaagaaa gaaaataaat tgcacccaca tatcttaatg agtaataatg acc 293 <210> 52 <211> 504 <212> DNA <213> Ishi 36 Bottlenosed <220> <221> CDO <222> (186) .. (412) <400> 52 aaacccattt taactagcag agtacttaat ttttcccata taacttatgt gaccaaataa 60 cacaatttct ttaaattaaa acatgctttt aatttttaca atttatataa aaattattgg 120 aagcaaacac tacttgaact tttctcatgt tttcaaataa ttattatcac taataaggtg 180 atcctgggct tccctggtgg cgcagtggtt gagagtccac ctgccgatgc aggggacacg 240 ggtttgtgcc ccggtccggg aagatacccc atgccgcgga gcggctgggc ccgtgaacca 300 tggccactgg gcctgcgcgt ccggagccgg tgctcgcaac gggagaggcc acaacagtga 360 gaggcccaag taccgcaaaa aaaaaaaaaa aaaaaaaaaa aaaggtgatt cttattatca 420 aactaccctt attaacagca aactatattt gataaatata taatatctat atacttctcc 480 cttcccaagt atatgaattg ttac 504 <210> 53 <211> 501 <212> DNA <213> Ishi 36 Short-finned <220> <221> CDO <222> (185) .. (409) <400> 53 aaacccattt taactgcaga gtacttaatt tttcccatat aacttatgtg accaaataac 60 acaatttctt taaattaaaa catgctttta atttttacaa tttatataaa aattattgga 120 agcaaacact acttgaactt ttctcatgtt ttcaaataat tattatcact aataaggtga 180 tcctgggctt ccctggtggc gcagtggttg agagtccacc tgccgatgca ggggacacgg 240 gtttgtgccc cggtccggga agatcccaca tgccgcggag cggctgggcc cgtgaaccat 300 ggccgctggg cctgcgcgtc cggagccggt gctccgcaac gggagaggtc acaacagtga 360 gaggcccaag taccgcaaaa aaaaaaaaaa aaaaaaaaaa ggtgattctt attatcaaac 420 tacccttatt aacagcaaac tatatttgat aaatatataa tatctatata cttctccctt 480 cccaagtata tgaattgtta c 501 <210> 54 <211> 512 <212> DNA <213> Ishi 36 Dall's <220> <221> CDO <222> (190) .. (420) <400> 54 aaacccattt taactgcaga gtacttaatt tttcccaaat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa acactacttg aacttttcac atggtttcaa ataattataa tcactaataa 180 ggtgatcctg ggcttccctg gtggcagtgg ttgagagtcc gcctgccgat gcaggggaca 240 tgggttcgtg ccctggtcsg gaagatccca catgccgtgg agcggctggg cccgtgagcc 300 atggccgctg agcctgcgcg tcaggagcct gtgctccgcg acgggagagg ccacaacagt 360 gagaggtccg cgtatcgcaa aaaaaaaata ataataataa taaaaaaaaa aggtgatact 420 tattatcaaa ctactcttat taacagcaaa ctatatttga taaatatata atatctatat 480 acttctccct tcccaagtat atgaattgtt ac 512 <210> 55 <211> 515 <212> DNA <213> Ishi 36 Narwhal <220> <221> CDO <222> (190) .. (423) <400> 55 gaacccattt taactgcaga gtactaaatt tttcccaaat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa acactacttg aacttttcac atggtttcaa acaattataa tcactgataa 180 ggtgatcctg ggcttccctg gtggcgcagt ggttgagagt ccgcctgccg atgcagggga 240 catgggttcg tgcctcggtc cgggaagatc ccacatgccg tggagagtct gggcccgtga 300 gccatggccg ctgagcctgc gcgtcaggag cctgtgctcc gcgacgggag aggccacaac 360 agtgagaggc ccgcgtatcg caaaaaataa taatgataat aataaaaaaa ataaggtgat 420 ccttattatc aaactactct tattaacagc aaactatatt tgataaatat ataatatcta 480 tatacttctc ccttcccaag tatatgaatt gttac 515 <210> 56 <211> 504 <212> DNA <213> Ishi 36 Amazon <220> <221> CDO <222> (190) .. (412) <400> 56 aaacccattt taactgcaga gtacttaatt tttcccatat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa acactacttg aacttttctc atggtttcaa ataattatta tcactaataa 180 ggtgatcctg ggcttccctg gtggcacagt ggttgagagt ctgcctgccg atgcagggga 240 cacgggttca tgccccggtc cgggaagatc ccacatgccg cggagcggct gggcccgtga 300 gccatggccg ctgagcctgc gcgtccggag cctgtgctcc acaacgggag aggccacaac 360 agtgagaggc ccgcgtacag caaaaaaata aataaataaa taaggtgatc cttattatca 420 aactaccctt attaacagca aactatattt gataaatata taatatctat acacttctcc 480 cttcccaagt atatgaattg ttac 504 <210> 57 <211> 501 <212> DNA <213> Ishi 36 La Plata <220> <221> CDO <222> (190) .. (409) <400> 57 aaacccattt taactgcaga gtacttaatt tttcccatat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaagtta 120 ttggaagtaa acactacttg aacttttctc atggtttcaa ataactatta tcactaataa 180 ggtgatcctg ggcttccatg gtggcacagt ggttgagagt ccgcctgccg attcagggga 240 cacgggttcg tgccccggtc cgggaagatc ccacgtgccg cagagcggct gggcccgtga 300 gccatggcaa ctgagcctgc gcatccggag cctgtgctcc gcaacgggag agaccacaac 360 agtgagaggc ccgcgtccgc aaaaaaaaaa aaaaaaaaaa ggtgatcctt attatcaaac 420 tacccttatt aacatcaaac tatatttgat aaatatataa tatctatata cttctccctt 480 cccaagtata tgaattgtta c 501 <210> 58 <211> 508 <212> DNA <213> Ishi 36 Baiji <220> <221> CDO <222> (189) .. (419) <400> 58 aacccatttt aactgcagag tacttaattt ttcccatata acttatgtga ccaaataaca 60 caatttcttt aaataaaaac atgcttttaa tttttacaat ttaactagta taaaaattat 120 tggaagcaaa cactacttga acttttctta tggtttcaaa taattattat cactaataag 180 gtgatcctgg gcttccctgg tggcgcagtg gttgagagtc cgcctgccga tgcaggggac 240 acgggttcgt gccccagtct gggaggatcc cacatgccgc ggagcggctg ggcccgtgag 300 ccatggccac tgagcctgca cgtccggagc ctgtgctccg caacgggaga ggcacaacag 360 tgagaggccc gcgtccgcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ggtgatcctt 420 attatcaaac tacccttatt aacagcaaac tatatttgat aaatatatat ctatatactt 480 ctcccttccc aagtatatga attgttac 508 <210> 59 <211> 281 <212> DNA <213> Ishi 36 Beaked <400> 59 aaacccattt taactgcaga gtacttaatt ttttccatat aacttatgcg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa atactacttg aacttttctc atgatttcaa ataattatta tcactaataa 180 ggtgatcctt attatcaaac tacccttatt aacagcaaaa tatatttgat aaatatataa 240 tatctatata cttctccctt cccaagtata tgaattgtta c 281 <210> 60 <211> 283 <212> DNA <213> Ishi 36 Ganges <400> 60 taaacccatt ttaactgcag agtacttaat ttttccctta taacttatgt gaccaaataa 60 cacaatttct ttaaataaaa acatgctttt aatctttaca atttaactag tataaaaatt 120 attggaagca aacactactt gaacttttct catggtttca aataattatt atcactaata 180 aggtgatcct tattatccaa ctacccttaa taacagcaaa ctatatttga tgaatatata 240 atatctatat acttctccct tcccaagtat atgaattgtt aca 283 <210> 61 <211> 281 <212> DNA <213> Ishi 36 Sperm <400> 61 aaacccattt taactgcaga gtacttaatt tttcccatat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa acactacttg aacttttctc atggtttcaa ataattatta tcactaataa 180 ggtgatcctt attatcaaac tacccttatt aacagcaaac tatatttgac aaatatataa 240 tatctatata cttctccctt cccaagtata tgaattgtta c 281 <210> 62 <211> 280 <212> DNA <213> Ishi 36 Humpback <400> 62 aaacccattt taactgcaga gtacttaatt tttcccatat aacttatgtg accaaataac 60 acaatttctt taaataaaaa catgctttta atttttacaa tttaactagt ataaaaatta 120 ttggaagcaa cactacttga actcttctca tggtttcaaa taattattat cactaataag 180 gtgatcctta ttatcaaact acccttatta acagcaaact atatttgata aatatataat 240 atctatatac ttctcccttc ccaagtatat gatttgttac 280 <210> 63 <211> 283 <212> DNA <213> Ishi 36 Minke <400> 63 taaacccatt ttaactgcag agtacttaat ttttcccata taacttatgt gaccaaataa 60 cacaatttct ttaaataaaa acatgctttt aatttttaca atttaactag tataaaaatt 120 attggaagca aacactactt gaactcctct catggtttca aataattatt atcactaata 180 aggtgatcct tattatcaaa ctacccttat taacagcaaa ctatatttga taaatatata 240 atatctatat acttctccct tcccaagtat atgaattgtt aca 283 <210> 64 <211> 282 <212> DNA <213> Ishi 36 Fin <400> 64 taaacccatt ttaactgcag agtacttaat ttttcccata taacttatgt gaccaaataa 60 cacaatttct ttaaataaaa acatgctttt aatttttgca atttaactag tataaaaatt 120 attggaagca aacactactt gaactcttct catggtttca aataattatt atcactaata 180 aggtgatcct tattatcaaa ctacccttat taacagcaaa ctatatttga taaatatata 240 atatctatat acttctccct tcccaagtat atgatttgtt ac 282 <210> 65 <211> 276 <212> DNA <213> Ishi 36 Hippo <400> 65 aaatccattt aactgtagag tacttaattt ttcccatata cttatgcgac caaatacaca 60 atttatttaa acaaaaacat gcttttaatc tttacaattt agctagtata aaaattactg 120 gaagcaaaca ctagttgaac ttttttcatg gtttcaaata attagtatca ttaataaaga 180 tccttattat caaactaccc ttattaatag caaactgtat ttgataaata tacaatatct 240 atattcttct cccttcccaa gtatatgaat tgttac 276 <210> 66 <211> 385 <212> DNA <213> Ishi 38 Bottlenosed <220> <221> CDO <222> (49) .. (278) <400> 66 tgaatcataa gctctcgtcc atgattttcc taataaaaga acacttgagg gcttccctgg 60 tggcgcagtg gttgagagtc tgcctgccga tgcaggtgac acgggttcat gccctggacc 120 gggaagatcc cacatgccgc gaagcagctg ggcccgtgag ccatggccgc tgagcctgcg 180 agtctggagc ctgtgctcca caacgggaga ggctacaaca gtgagaggcc cgcgtaccgc 240 aaaaacaaaa caaaacaaaa caaacaaaaa acacctgagg ctgtttattc atttacagct 300 taaaaaagaa aaaggagtcc tgccccttga tatatataca tctcaaacaa atccttggtt 360 ccaatctgga ctttcatgtg tttat 385 <210> 67 <211> 393 <212> DNA <213> Ishi 38 Short-finned <220> <221> CDO <222> (49) .. (286) <400> 67 tgaatcataa gctctcgtcc atgattttcc taataaaaga acacctgagg gcttccctgg 60 tggcgcagtg gttgagagtc tgcctgccga tgcaggtgac acgggttcat gccctggacc 120 gggaagatcc cacatgccgc gaagcggctg ggcccgtgag ccatggccgc tgagcctgcg 180 cgtctggagc ctgtgcgcca caacgggaga ggccacaaca gtgagaggcc cgcgtaccgc 240 aaaaacaaaa caaaacaaaa caaacaaaaa aacaaaaaac acctgaggct gtttattcat 300 ttacagctta aaaaagaaaa aggagtcctg tcccttgata tatatacatc tcaaacaaat 360 ccttggttcc aatctggact ttcatgtgtt tat 393 <210> 68 <211> 390 <212> DNA <213> Ishi 38 Dall's <220> <221> CDO <222> (50) .. (283) <400> 68 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag ggcttccctg 60 gtggcgcagt ggttgagagt ccgcctgcca atgcagggga cacgggttca tgccctggac 120 cgggaagatc ccacatgccg tgaagcggct gggcccgtga gccatggccg ctgagcctgc 180 gcgtctggag cctgtgctcc acaacgggag aggccacaac agtgagaggt ctgcgtaccg 240 caaaaacaaa acaaaacaaa aaaaaacaaa acaaaacacc tgaggctgtt tattcattta 300 cagccttaaa aagaaaaagg agtcctgccc cttgatatat atacatctca aacaaatcct 360 tggttccaat ctggactttc atgtgtttat 390 <210> 69 <211> 393 <212> DNA <213> Ishi 38 Narwhal <220> <221> CDO <222> (49) .. (286) <400> 69 tgaatcataa gctctcgtcc atgattttcc taataaaaga acacctgagg gcttccctgg 60 tggcgcagtg gttgagagtc cgcctgccga tgcaggggac acgggttcat gccccggtcc 120 gggaagatcc cacatgccgc ggagtggctg ggcccgtgag ccatggccgc tgagcctgcg 180 cgtctggagc ctgtgctcca caacgggaga ggccacaaca gtgagaggcc tgcgtaccgc 240 aaaaacaaaa caaaacaaaa caaaacaaaa caaaaaaaac acctgaggct gtttattcat 300 ttacagcctt aaaaagaaaa aggagtcctg ccccttgata tatatacatc tcaaacaaat 360 ccttggttcc aatctggact ttcatgtgtt tat 393 <210> 70 <211> 369 <212> DNA <213> Ishi 38 Amazon <220> <221> CDO <222> (50) .. (262) <400> 70 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag ggcttccctg 60 gtggtgcagc ggttgagagt ccgcctgccg atgcaggtga cacgggttca tgccctggac 120 cgggaagatc ccatatgccg cagagcgctg ggcccgtgag ccatggctgc tgagcctgcg 180 cgtccggagc ctgtgctcca cgacgggaga ggccacaaca gtgagaggcc cgcgtaccgc 240 aaaaaaaaaa aagaacacct gaggctgttt attcatttac agcttaaaaa agaaaaaggg 300 ggcctgcccc ttgatatata tacatctcaa acaaatcctt ggttccaatc tggactttca 360 tgtgtttat 369 <210> 71 <211> 367 <212> DNA <213> Ishi 38 La Plata <220> <221> CDO <222> (50) .. (260) <400> 71 ttgaatcata agctctcgtc catgattttc ctagtaaaag aacacctgag ggcttccctg 60 gtggcgcagt ggttgagagt ccgcctgccg atgcaggtga cacgggttcg tgccctggac 120 cgggaagatc ccacatgcca cagagcggct gggcccgtga gccatggctg ctgagcctgc 180 gcgtcccgag cctgtgctcc acaacgggag aggccacaac agggagaggc ccgcgtaccg 240 ccaaaaaaaa gaacacctga ggctgtttat tcatttacag cttaaaaaag aaaaaggagt 300 cctgcccctt gatatatgta catctcaaac aaacccttgg ttccaatctg gactttcatg 360 tgtttat 367 <210> 72 <211> 388 <212> DNA <213> Ishi 38 Baiji <220> <221> CDO <222> (49) .. (281) <400> 72 tgaatcataa gctctcgtcc atgattttcc taataaaaga acacctgagg gcttccctga 60 tggcgcagtg gttgagagtc cgcctgccga tgcaggtgac acgggttcat gccctggacc 120 gggaagatcc cacatgccgc ggagcagctg ggcccgtgag ccatggccgc tgagcctgcg 180 cgtctggagc ctgtgctcca caatgggaga ggccacaaca gtgagaggcc cgtgtaccac 240 aaaaacaaaa caaaacaaaa caaaagaaac aaaacacctg aggctgttta ttcatttaca 300 gcttaaaaaa gaaaaaggac tcctgcccct tgatatatat acatctcaaa caaatccttg 360 gttccaatct ggactttcat gtgtttat 388 <210> 73 <211> 155 <212> DNA <213> Ishi 38 Beaked <400> 73 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag gctgtttatt 60 catttacagc ttaaaaaaaa aaaagagtcc tgccccttga tatatataca tctcaaacaa 120 atccttggtt ccaatctgga ctttcatgtg tttat 155 <210> 74 <211> 159 <212> DNA <213> Ishi 38 Ganges <400> 74 ttgaatcata agctctcgtc catgattttc ctaattaaag aacacctgag gctgtttatt 60 catttacagc ttaaaaaaaa aaaaaaagga gtcctgaccc ttgatatata tacatctcaa 120 acaaatcctt agttccaatc tggactttca tgtgtttat 159 <210> 75 <211> 156 <212> DNA <213> Ishi 38 Sperm <400> 75 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag gctgtttatt 60 catttacact ttaaaaaaaa aaaaagagtc ctgccccttg atatatatac atctcaaaca 120 aatccttggt tccaatctgg actttcatgt gtttat 156 <210> 76 <211> 154 <212> DNA <213> Ishi 38 Humpback <400> 76 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag gctgtttatt 60 catttacagc ttaaaaaaaa aaagagtcct gccccttgat atatatacat ctcaaacaaa 120 tccttggttc caatctggac tttcatgtgt ttat 154 <210> 77 <211> 154 <212> DNA <213> Ishi 38 Fin <400> 77 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag gctgtttatt 60 catttacagc ttaaaaaaaa aaagagtcct gccccttgat atatatacat ctcaaacaaa 120 tccttggttc caatctggac tttcatgtgt ttat 154 <210> 78 <211> 152 <212> DNA <213> Ishi 38 Minke <400> 78 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacctgag gctgtttatt 60 catttacagc ttaaaaaaaa agagtcctgc cccttgatat atatacatct caaacaaatc 120 cttggttcca atctggactt tcatgtgttt at 152 <210> 79 <211> 154 <212> DNA <213> Ishi 38 Hippo <400> 79 ttgaatcata agctctcgtc catgattttc ctaataaaag aacacttgag agtgtttatt 60 cattaggaat gaaggaggat gaaggagtcc tgccccttga tatatataaa tccaaacaaa 120 tccttggttc caatctggac tttcatgtgt ttat 154 <210> 80 <211> 402 <212> DNA <213> Mago 32 Bottlenosed <220> <221> CDO <222> (62) .. (282) <400> 80 cactcattca tgtacaccca gatacaaaca tctatatatt tactttataa aagtggaatt 60 agggcttccc tggtagcaca gtggttgaga gtccgcctac caatgcaggg gacacgggtt 120 cgtgccccgg tctgggaaga tcccacatgc cacggagcgg ctgggcccgt gagccatggc 180 cgctgagcct gtgcatctgg agcctgtgct ccacaacggg agaggccaca gcggtgagag 240 gcccgcatac cgcaaaaaaa aaaaaaaaaa aaagtggaat tattctaaac attgaagtct 300 gaaacttgct tttcttcccc acataataaa ccagtgatat ctttctagga caacacatga 360 gctatttctc atggttttta atggctgcat tatgttccac tt 402 <210> 81 <211> 399 <212> DNA <213> Mago 32 Short-finned <220> <221> CDO <222> (61) .. (279) <223> "n" means undetermined. <400> 81 cactcattca tgtacaccca gatacaaaca tctatatatt tactttataa aagtgaatta 60 ggcttccctg gtagnacagt ggttgagagt ccgcctgcca atgcagggga cacgggtttg 120 tgccccggtc tgggaagatc ccaccatgcc ggagcggctg ggcccgtgag ccatggccgc 180 tgagcctgtg catctggagc ctgtgctcca caacgggaga ggccacagcg gtgagaggcc 240 cgcataccgc aaaaaaaaaa aaaaaaaaaa gtgggattat tctaaacatt aaagtctgaa 300 acttgctttt cttccccaca caataaacca gtgatatctt tctaggacaa cacatgagct 360 atttctcatg gtttttaatg gctgcattat gttccactt 399 <210> 82 <211> 400 <212> DNA <213> Mago 32 Dall's <220> <221> CDO <222> (61) .. (280) <400> 82 actcattcat gtacacccag atacaaacat ctatatattt actttataaa agtggaatta 60 gggcttccct ggtggcgcag tggttgagag tccacctgcc aatgcagggg acatgggttc 120 gtgccccggt ccgggaagat ccaacatgct gcggagggct gggcccggga gccatggccg 180 ctgagcctgt gtgtctggag tctgtgctcc gcaacgggag aggccacagc ggtgagaggc 240 ccgcgtaccg caaaaaaaaa aaaaaaaaaa agtggaatta ttctaaacat taaagtctga 300 aacttgcttt tcttccccac ataataaacc agtgatatct ttctaggaca atacatgagc 360 tatttctcat ggtttttaat ggctgcatta tgttccactt 400 <210> 83 <211> 405 <212> DNA <213> Mago 32 Narwhal <220> <221> CDO <222> (61) .. (285) <400> 83 cactcattca tgtacaccca gatacaaaca tctatatatt tactttataa aagtggaatt 60 agggcttccc tggtggcgca gtggttgaga gtccacctgc caatgcaggg gacacgggtt 120 tgtgccccgg tccgggaaga tcccacatgc tgcggagcgg ctgggcccgt gagccatggc 180 cgctgagcct gtgcgtctgg agcctgtgct ccgcaacggg agaggccaca gcggtgagag 240 gccccgtacc gcaaaaaaaa aaaaaaaaaa aaaaaagtcg aattattcta aacattaaag 300 tctgaaactt gcttttcttc cccacataat aaaccagtga tatctttcta ggacaacaca 360 tgagctattt ctcatggttt ttaatggctg cattatgttc cactt 405 <210> 84 <211> 417 <212> DNA <213> Mago 32 Amazon <220> <221> CDO <222> (101) .. (329) <400> 84 gtagtttggt acaatttcat tcctactctc tctcacattc actcattcat gcacacccag 60 atacaatcat ctatatattt actttataaa agtggaatta gggcttccct ggtggcgcag 120 tggttgagag tccacctgcc aatgcagggg acatgggttc gtgccccagt ccgggtaaga 180 tcccacgtgc cgtggagtgg ctgggcccat gagccatggc cgctgagcct gtgcgtccgg 240 agcctgtgct ccgcagtggg agaggccaca gaggtgagag gcccgcgtac cgcaaaaaaa 300 acaaaaaaca aaaaacaaaa gtggaattat tctaaacatc agagtccgaa acttgctttc 360 cttccccaca taataaacca gtgatatctt tctaggacaa cacatgagct atttcta 417 <210> 85 <211> 397 <212> DNA <213> Mago 32 La Plata <220> <221> CDO <222> (100) .. (314) <400> 85 tagtttggta caatttcatt cctactctct ctcacattca ctcattcatg tacacccaga 60 tacaaacatc tatatattta ctttataaaa gtggaattag ggcttccttg gtggcgcagt 120 ggttgagagt ccgcctgcca atgcagggga cacgggttcg tgccccagtc cgggaagatc 180 ccacgtgccg tggagcggct gggcccatga gccatggccg ctgagcctgt gcgtccggag 240 cctgtgctcc gcaacgggag aggccacaac agtgagaggc ccgcgtacca caaaaaaaaa 300 caaaagtgga attattctaa acattagagt ctgaaacttg cttttcgtcc ccacataata 360 aaccagtgat atctttctag gacaacacat gagctat 397 <210> 86 <211> 401 <212> DNA <213> Mago 32 Baiji <220> <221> CDO <222> (62) .. (281) <400> 86 cactcattca tgtacaccca gttacaaaca tctatatatt tactttataa aagtggaatt 60 agggcttccc tggtggcgca gtggttgaga gtccacctgc caatgcaggg gacacgggtt 120 ggtgccccag tccgggaaga tcccacatgc cgtggagcgg ctgggcccgt gagccatggc 180 cgctgagcct gtgcgtccgg agcctgtgct ccgcaatgag agaggccaca gtggtgagag 240 gcccgcgtac cacaaagaaa caaaaaacaa aagtggaatt attctaaaca ttagagtctg 300 aaacttgctt ttcttcccca cataataaac cagtgatatc tttctaggac aacacatgag 360 ctatttctca tggtttttaa tggctgcatt atgttccact t 401 <210> 87 <211> 184 <212> DNA <213> Mago 32 Beaked <400> 87 tatagtttgg tacaatttca ttcctactct cacactcact cattcatgta cacccagata 60 caaacatcta tatatttact ttataaaagt ggaattactc taaacattag aatctgaaac 120 ttgctttttt tccccacata ataaaccagt gatatctttc taggacaaca catgagctat 180 ttct 184 <210> 88 <211> 171 <212> DNA <213> Mago 32 Ganges <400> 88 ttcattccta ctctctctca ctcactcatt catgtatacc cagatacaaa catctataca 60 tttactttat aaaagtggaa ttattctaaa cattagagtc tgaaacttgc ttttttcccc 120 cacataataa accagtgata tctttccagg acaacacatg agctatttct a 171 <210> 89 <211> 188 <212> DNA <213> Mago 32 Sperm <400> 89 tatagtttgg tacaatttca ttcctactct ctctcacact cactgattca tgtacaccca 60 gatacaaaca tctatatatt tactttacaa aagtggaatt attctaaaca ctagagtctg 120 aaacttgctt ttttccccca cataataaac cagtgatatc tttctaggac aacacatgag 180 ctatttct 188 <210> 90 <211> 181 <212> DNA <213> Mago 32 Humpback <400> 90 cactcattca tgtacaccca gatacaaaca cctatatatt tactttacaa aagtggaatt 60 attctaaaca ttagagtctg aaacttgctt tttttcccca cataataaac cagtgatatc 120 tttctaggac aacacatgag ctatttctca tggtttttaa tggctgcatt atgttccact 180 t 181 <210> 91 <211> 148 <212> DNA <213> Mago 32 Fin <400> 91 cactcattca tgtacaccca gatacaaaca cctatatatt tactttacaa aagtggaatt 60 attctaaaca ttagagtctg aaacttgctt tttttcccca cataataaac cagtgatatc 120 tttctaggac aacacatgag ctatttct 148 <210> 92 <211> 181 <212> DNA <213> Mago 32 Minke <400> 92 cactcattca tgtacaccca gatacaaaga cctatatatt tactttacaa aagtggaatt 60 attctaaaca ttagagtctg aaacttgctt tttttcccca cataataaac cagtgatatc 120 tttctaggac aacacatgag ctatttctca tggtttttaa tggctgcatt atgttccact 180 t 181 <210> 93 <211> 190 <212> DNA <213> Mago 32 Hippo <400> 93 tatagtttgg tacaatttca ttcctactct gctccacact cactcactca tgcacaccct 60 agatactaaa catctgtaga tttattttac aaaaatggaa ttactcaaaa cattaaagtc 120 tgaaacttgc cttttttccc cacataatac accagtgata tctttctagg acaacacatg 180 agctatttct 190 <210> 94 <211> 405 <212> DNA <213> Mago 8 Short-finned <220> <221> CDO <222> (92) .. (315) <400> 94 tgctaactct agattgcaat gaaccaaaat tgaaaggaaa agagaatctt atttccaggc 60 acactcttat gtatataaaa tgctatcatg ggggcttccc tggtggcgca gtggttgaga 120 gtccgcctgc cgatgcaggg gacatgggtc tgtgcctcat ccgggaagat cccacatgcc 180 gtggagcggc tgggcccgtg agccatggcc cctgagcctg cgcgtctgga gcctgtgctc 240 cgcaacggga gaggccacaa cagtgagagg cccgcatacc gcaaaaaaaa aaaaaaaaaa 300 aaaaagctat catggtctac aataaaaata atcaataagt tttatcaaat gctaccatgt 360 tcatggtact gtttatttag aaaggctcaa tcacgaaaaa ttccc 405 <210> 95 <211> 398 <212> DNA <213> Mago 8 Beaked <220> <221> CDO <222> (90) .. (310) <400> 95 tgctaactct agattgcaat gaaccaaaat tgaaagaaaa agaatcttat ttccaggcac 60 attcttatgt atataaaatg ctatcatggg ggcttccctg gtggtgcagt ggttgagagt 120 ccgcctaccg atgcagggga cacgggttcg tgcccaggtc cgggaagatc ccacatgcgc 180 ggagcggctg ggcccgtgag ccatggccgc tgagcctgcg cgtctggagc ctgtgctccg 240 caatgggaga ggtcacaaca gtgagaggcc cacataccgc aaaaaaaaaa aaaacacaat 300 gctatcatgg tctacaatca aaaaatcagt atgttttatt aaatgctacc atgttcatgg 360 tgctgtttat ttagaaaggc tcaatcacga aaattccc 398 <210> 96 <211> 181 <212> DNA <213> Mago 8 Ganges <400> 96 tgctaactct agattgcaat gaaccaaaat taaaagaaaa agagaatctt atttccaggc 60 acattcttat gtatataaaa tgctatcatg gtctacaatc aaaataatca ataagtttta 120 ttaaatgtta ccacgttcat ggtactgttt atttagaaag gctcaatcac gaaaaattcc 180 c 181 <210> 97 <211> 182 <212> DNA <213> Mago 8 Sperm <400> 97 tgctaactct agattgcaat gaaccaaaat tgaaagaaaa agagaatctt atttccaggc 60 acattcttat gtatataaaa tgctatcatg tttacgatca aagtaatcaa taagttttat 120 ttaaatgcta ccatgttcat ggtactgttt atttagaaag gctcaatcac gaaaaaattc 180 cc 182 <210> 98 <211> 178 <212> DNA <213> Mago 8 Humpback <400> 98 tgctaactct agattgcaat gaaccaaaat tgaaagaaaa agagaatctt atttccaggc 60 acattgctta tgtatataaa atgctatcat ggtctacaat caaaataata agttttatta 120 aatgctacca tgttcatggt actgtttatt tagaaaggct caatcacgaa aaattccc 178 <210> 99 <211> 320 <212> DNA <213> Mago 13 Bottlenosed <220> <221> CDO <222> (50) .. (268) <400> 99 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtggcacagt ggttgagggt ccgcctgccg atgcagggga cacgggttca tgccccagtc 120 cgggaagatc ccacatgccg cagagcggct aggcccgtga gccatggcca gtgagcctgc 180 gcatccggag cctgtgctcc gccatgggag aggccacaac agtgagaggc ccgcgtaccg 240 caaaaaaaaa aaaaaaatag aggatctcca gctataaaac ctctaaataa agttgtaatg 300 ccagcaaaag tacagcccag 320 <210> 100 <211> 321 <212> DNA <213> Mago 13 Short-finned <220> <221> CDO <222> (50) .. (270) <400> 100 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtggcacagt ggttgagggt ccgcctgccg atgcagggga cacgggttca tgccccagtc 120 cgggaagatc ccacatgccg cagagcgcta ggcccgtgag ccatggccag tgagcctgcg 180 cgtccggagc ctgtgctccg ccatgggaga ggccacaaca gtgagaggcc cgcgtaccgc 240 aaaaaaaaaa aaaaaaaaat agaggatctc cagcataaaa cctctaaata aagttgtaat 300 gccagcaaaa gtacagccca g 321 <210> 101 <211> 342 <212> DNA <213> Mago 13 Dall's <220> <221> CDO <222> (50) .. (289) <400> 101 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtggcgcagt ggttgagggt ccgcctgcca atgcagggga catgggttcg tgccccggtc 120 cgggaagatc ccacatgccg cagagcggct aggcccgtga gccatggccg gtgagcctgc 180 gcgtccggag cctgtgctcc acacgggaga ggccacaaca gtgagaggcc cgcgtaccgc 240 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaata gaggatctcc tagctataaa 300 acctctaaat aaagttgtaa tgccagcaaa agtacagccc ag 342 <210> 102 <211> 317 <212> DNA <213> Mago 13 Narwhal <220> <221> CDO <222> (50) .. (266) <400> 102 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtggcgcagt ggttgagggt ccgcctgcca atgcagggga catgggttca tgccccggtc 120 cgggaagatc ccacatgccg cagagcggct aggcccgtga gccatggccg gtgagcctgc 180 ccgtccagag cctgtgctct gccacgggag aggccacaac agtgagaggc ctgcgtaccg 240 caaaaaaaaa aaaaagagag gatctccagc ataaaacctt taaataaagt tgtaatgcca 300 gcaaaagtac agcccag 317 <210> 103 <211> 300 <212> DNA <213> Mago 13 Amazon <220> <221> CDO <222> (50) .. (247) <400> 103 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtaggcgcag tggttgaggg tccgcctgcc aatgcagggg acatgggttc atgccccagt 120 ccgggagcag gtaggcccgt gagccatggc cactgagcct gcgcgtccgg agcctgtgct 180 ccacaacagg agaggccaca acagtgacag gtccgcgtac cgcaaaaaaa aaaaaaaaga 240 ggatctccag ctataaaacc tctaaataaa gttgtaatgc ctagcaaaag tacagcccag 300 <210> 104 <211> 308 <212> DNA <213> Mago 13 La Plata <220> <221> CDO <222> (50) .. (255) <400> 104 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcg ggcttccctg 60 gtggcgcagt ggttgagggt ccgcctgcca atgcagggga cacgggttcg tgccccggtc 120 cgggagcggc taggcccgtg agccatggcc gctgagcctg cgcgtccgga gcctgtgctc 180 cgcaatggga gaggccacaa cagagaggtc cgcgtaccgc aaaaaaaaaa aaaaaaaaaa 240 aaaaaagagg atctccagct ataaaacctc taaataaagt tgtaatgcct agcaaaagta 300 cagcccag 308 <210> 105 <211> 293 <212> DNA <213> Mago 13 Baiji <220> <221> CDO <222> (50) .. (242) <400> 105 aaaaaatgtt tctatcacta tatacaatat ttttaaaata gaggatctcg ggcttccctg 60 gtggcgcagt ggttgagggt ccacctgccg atgcagggga cacgggttcg tgccccggtc 120 agggagtggc taggcccgtg agccatggcc actgagcctg cgcgtccgga gcctgtgctc 180 cgcaacggga gaggccacaa cagtgagagg tctgcgtacc gcaaaaaaaa atagaggatc 240 tccagcataa aacctctaaa taaagttgca atgccagcaa aagtacagcc cag 293 <210> 106 <211> 317 <212> DNA <213> Mago 13 Beaked <220> <221> CDO <222> (50) .. (266) <400> 106 aaaaaatgtt tctatcacta tctacaatat ttaaaaaata gaggatctca ggcttctctg 60 gtggtgcagt ggttgagggt ccgcctgccg atgcagggga cacgggtttg tgccccggtc 120 cgggaagaat cccacatgcc gcagagcgct gggcccgtga gccatggccg ctgagcctgc 180 acatccggag cctgtgctcc gcaacgggag aggccacaac agtgagaggc ctgcgtacca 240 caaaaaaaaa aaaaaaagag gatctccagc ataaaacctc tgaataaagt tgtaatgcca 300 gcaaaagtac agcccag 317 <210> 107 <211> 100 <212> DNA <213> Mago 13 Ganges <400> 107 aaaaaatgtt tctatcacta tctacaatat ttttaaaata gaggctctcc agcataaaac 60 ctctaaataa agttgtaatg ccagcaaaag tacagcccag 100 <210> 108 <211> 100 <212> DNA <213> Mago 13 Sperm <400> 108 aaaaaatgtt tctatcacta tctacaatat tttaaaaata gaggatctcc agcataaaac 60 ctctaaataa agttgtaatg ccagcaaaag tacagcccag 100 <210> 109 <211> 100 <212> DNA <213> Mago 13 Humpback <400> 109 aaaaaatgtt tctatcacta tctacaatac tttaaaaata gagaatctcc agcataaaac 60 ctctaagtaa agttgtaatg ccagcaaaag tacagcccag 100 <210> 110 <211> 100 <212> DNA <213> Mago 13 Fin <400> 110 aaaaaatgtt tctatcacta tctacaatac tttaaaaata gaggatctcc agcataaaac 60 ctctaagtaa agttgtaatg ccagcaaaag tacagcccag 100 <210> 111 <211> 100 <212> DNA <213> Mago 13 Minke <400> 111 aaaaaatgtt tctatcccta tctacaatac tttaaaaata gaggatctcc agcataaaac 60 ctctaaataa agttgtaatg ccagcaaaag tacagcccag 100 <210> 112 <211> 99 <212> DNA <213> Mago 13 Hippo <400> 112 aaaaatgttt ctatcactat ctacaatatt ttaaaaatag aggatctcca gcataaaacc 60 tctaaataaa gttgtaatgc cagcaaaagt acagcccag 99 <210> 113 <211> 370 <212> DNA <213> Mago 21 Short-finned <220> <221> CDO <222> (63) .. (289) <400> 113 ccttcttcat caggttatta ggaagattcc gatagttaat ataaaatata taaagtattt 60 aggggcttcc ctggtggcgc agtggttgag agtccacctg ccgatgcagg ggacacaggt 120 tcgtgccctg gtccgggagg atgccacatg scgcagagca gctgggcccg tgagccatgg 180 ccgctgagcc tgagcatctg gagcctgtgc tccgcaatgg gagaggccac aacagtgaga 240 ggcccacgta ccgcaaaaaa aaaaaaatat atatatatat agtatttaga acagagttta 300 gaacacagta agtactatgt tagtgttagc ttttatagat gtgattatct agtgggattt 360 gttgatttcc 370 <210> 114 <211> 373 <212> DNA <213> Mago 21 La Plata <220> <221> CDO <222> (63) .. (292) <400> 114 ccttcttcat caggttatta ggaagattcc gttagttcat ataaaatata taaagtattt 60 aggggcttcc ctcgtggtgc agtggttgag agtccgcctg ccgacgcagg ggacgtgggt 120 ccgggaagat cccacatgcc gtggagcggc tgggcccgtg agccatggcc gctgagcctg 180 agcgtccgga gcctgtgctc tgcaatggga gaggccacaa cagtgagagg cctgtgtacc 240 gcaaaaaaaa aaattttttt aataaacaaa tatatatata tatagtattt agaacagagt 300 ttagaacaca gtaagtacta tgttagtgtt agcttttata gatgtgatta tctagtggga 360 tttgttgatt tcc 373 <210> 115 <211> 406 <212> DNA <213> Mago 21 Ganges <220> <221> CDO <222> (63) .. (325) <400> 115 ccttcttcat caggttatta ggaagattcc attagttaat ataaaatata taaagtattt 60 aggggcttct ctggtggcgc agtggttgag agtccacctg ccgatgcagg ggacacgggt 120 tcgtgccctg gtcccggaag atcccacatg tgcggagcag ctggtcccgt gagccacggc 180 cgctgagcct gcgcgtccgg agcctgtstc cgcaacggga aaggccacaa cagtgagagg 240 cccgcttacc gcaaaaaata aaacatacat acatatatac atatagatat agatgtagat 300 atagatagat agataaagta tttagaacag agtttagaac acagtaagta ctatgttagt 360 gttagttttt atagatgtga ttatctagtg ggatttgttg atttcc 406 <210> 116 <211> 143 <212> DNA <213> Mago 21 Sperm <400> 116 ccttcttcat caggttatta ggaagattcc gttagttaat agaaaatatg taaagtattt 60 aggacggagt ttagaacaca gtaagtacta tgttagtgtt agcttttata gatgtgatta 120 tctagtggga tttgttgatt tcc 143 <210> 117 <211> 144 <212> DNA <213> Mago 21 Humpback <400> 117 ccttcttcat caggttatta ggaagattcc gttagttaat gtaaaatata taaagtattt 60 agaacagagt ttagaacaca gtaagtacta tgttagtagt tagcttttac agatgtgatt 120 atgtagtggg atttgttgat ttcc 144 <210> 118 <211> 526 <212> DNA <213> Sperm 8 Dall's <220> <221> CD <222> (154) .. (495) <400> 118 gccaatctct gtatttgttc atcaacagaa aaagggatat gaagtatgag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcaccacctt agtaacctcc 120 agtagatcat gtctacttat aaaaatttgt gttgggcttc cctggtggca cagtggttga 180 gaatctgcct gccaatgcag gggacacggg ttcgagccct ggtctgggaa gatcccacat 240 gccgcgaagc aactagggcc gtgagccaca actactgagc ctgcgcgtct ggagcctgtg 300 ctcctcaaca agagaggcca cgatagtgag aggcccgtgc accgcgatga agagtggccc 360 ctgcttgcca caactagaga aagccctcgc acagaaacaa agacccaaca cagccaaaaa 420 taaataaata aataaaaatt taaaaaagta acatgttcaa gtacaattgg tgtctttaaa 480 aaacaaatct atgtttttct atgtaagaca tgcaggtagg tggaca 526 <210> 119 <211> 533 <212> DNA <213> Sperm 8 Narwhal <220> <221> CD <222> (154) .. (502) <400> 119 gccaatctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcaccacctt agtaacctcc 120 agtagatcat gtctacttat aaaaatttgt gttgggcttc cctggtggcg cagtggttga 180 gaatctgcct gccaatgcag gggacacggg ttcgagccct gggctgggaa gatcccacat 240 gcagcaaagc aactaggccc gtgagccaca actactgagc ctgcgcgtct ggagcctgtg 300 ctcctcaaca agagaggcca cgatagtgag aagcccgtgc accgcgatga agagtggccc 360 ccgcttgcca caactagaga aagccctcgc acagaaacga agacccaaca cagtgaaaga 420 taaataaata aattaattaa ttaataaaaa agtaacaaat gttcaagtac aattggtgtc 480 tttaaagaaa aaaatctgtg tttttctatg taagacatgc aggtaggtgg aca 533 <210> 120 <211> 545 <212> DNA <213> Sperm 8 Amazon <220> <221> CD <222> (154) .. (514) <400> 120 gccaatctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcacctcctt agtaacctcc 120 agtggatcat gtctacttac aaaaatttgt gttgggcttc cctggtggcg cagtggttga 180 gaatctgcct gccaatgcag gggacacggg ttcgagccct ggtctgagaa cataccacat 240 accacggagc aactaggccc gtgagccaca actgagcctg catgtctgga gcctgtgctc 300 tgcaacaaga gaggccacga tagtgacagg cccgtgcacc gcgatgaaga gtggcccccg 360 cttgccacaa ctagagaaag ccctctcaca gaaatgaaga cccaacacag ccaaaaataa 420 ataaataaat aaatataaat aaataaataa aatttaaaaa aaagtaacaa gtgttcaagt 480 acaattggtt tcttaaaaaa aaaaaatctg tgtttttcta tgtaagacat gcaggtaggt 540 ggaca 545 <210> 121 <211> 535 <212> DNA <213> Sperm 8 La Plata <220> <221> CD <222> (154) .. (504) <400> 121 gccaatctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcacctcctt agtaacctcc 120 agtagaccat gtctacttat aaaaatgtgt gttgggcttc cctggcggcg cagtggttga 180 gaatttgcct gccaatgcag gggacatggg ttcgagccct ggtctgggaa gatcccacat 240 accgcggagc aactaggacc gtgagccaca actactgagc ctgcgcgtct ggagcctgtg 300 ctccgcaaca agagaggcca cgatagtgag aggcccgtgc accgcgatga agagtggccc 360 ccgcttgcca caactacaga aagctctctc acagaaacga agacccaaca cagccaaaaa 420 taaataaata aataaataaa ttttttttaa aaagtaacaa atgttcaagt acaattggtg 480 acttaaaaaa aaaaaatctg tgtttttcta tgtaagacat gcaggtaggt ggaca 535 <210> 122 <211> 529 <212> DNA <213> Sperm 8 Baiji <220> <221> CD <222> (151) .. (498) <400> 122 gccaatctct gtatttgttc atcaatagaa aaggatgtga agtgttagat aaaagatttc 60 tctaacttat ctactcattc ccctcctcca cactctctca cctccttagt aacctccagt 120 agatcatggc tacttataaa gatctgtgtt ggtctttcct ggtgatgcag tggttgagaa 180 tctgcctgcc aatgcagggg acacgggttc gagccctggt ctgggaagat cccacaagcc 240 gcggagcaac taggcccgtg agccacaact actgagcctg cgcgtctgga gcctgtgctc 300 cgcaacaaga caggccgcga tagtgagagg cccgcgcacc gcgatgaaga gtggcccccg 360 cttgccgcaa ctagagaaag ccctcgcaca gaaacgaaga cccaacacag ccaaaaataa 420 ataaattaat taattttttt aaaaaagtaa caaatgttca agtacaattg gtgtctttaa 480 aaaaaagaaa tctgtgtttt tctatgtaag acatgcaggt aggtggaca 529 <210> 123 <211> 539 <212> DNA <213> Sperm 8 Beaked <220> <221> CD <222> (154) .. (508) <400> 123 gccaatcttt gtatttgttc atcaatagaa aaatggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcaccttctt agtaacctcc 120 agtagatcat ggctacttat aaaaatctgt gttgggcttc cctggtggca cagtggttga 180 gaatctgcct gccaatgcag gggacacggg ttcgagccct ggtctgggaa gatcccacat 240 accgtggagc aactaggccc atgagccaca actactgagc ctgcgcgtct ggagcttgtg 300 ctctgcaaca agagaggctg cgatagggag aggcctgcgc accgtgatga agagtggccc 360 ccgctcgcca caactagaga aaaccctcgc acagaaatga agacccaaca cagccaaaaa 420 taaataaata aataaataaa taaataaaaa tttaaaaaag taacaaatgt tcaagtacaa 480 ttggtgtctt taaaaaaaaa tctgtgtttt tctatgtaag acatgcaggt aggtggaca 539 <210> 124 <211> 535 <212> DNA <213> Sperm 8 Ganges <220> <221> CD <222> (155) .. (504) <400> 124 gccaatctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcaccttctt ggtaaacctc 120 cagtaggtca tggctactta taaaaatctg tgttgggctt ccctggtggc gcagtggttg 180 agaatcttcc tgccgatgca gggaacatgg gtttgagccg tgctctggga agatcccaca 240 tgccacggag caactaggcc cgtgagccac aattactgag cctgcgcgtc tggagcctgt 300 gctccacaac aagagaggcc gcgataatga gaggcccgcg catcgcgatg aagagtggcc 360 ctgctcgccg caactagaga aagccctcgc acagaaacga agacccaaaa cagccaaaaa 420 taaataaata aattttttaa aaaaagtaac aaatgttcaa gtacaattgg tgtctttttt 480 tttttttttt ttttaatctg tgtttttcta tgtaagacat gcaggtaggt ggaca 535 <210> 125 <211> 526 <212> DNA <213> Sperm 8 Sperm <220> <221> CD <222> (154) .. (495) <400> 125 gccaatctct gtatttgttc atcaatagaa aaagggatat gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct ccacactctc tcaccttctt agtaacctcc 120 aacagatcat ggctacttat aaaaatctgt gttgggcttc cctggtggcg cagtggttga 180 gaatctgcct gctaacgcag gggacacggg ttcgagccct ggtctgggaa gatcccacat 240 gccgcggagc aactagacac gtgagccaca actactgagc ctgcgcgtgt ggagcctgtg 300 ctccgcaaca agagaggccg cgatagtgag aggcccgcgc actgcgatga agagtggccc 360 ccgcttgcca caactagaga aagcccttgc acagaaacga agacccaaca cagcgataaa 420 taaataaata gttttttaaa aaaaagtaac aaatattcaa gtacaattgg tgtctttaaa 480 aaaaaaatct gtgtttttct atgtaagaca tgcaggtagg tggaca 526 <210> 126 <211> 184 <212> DNA <213> Sperm 8 Humpback <400> 126 gccaatctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaatt tatctactca ctccccttct ccacactcta tcaccttctt agtaacctcc 120 agtagatcat ggctacttat aaaaatctgt gtttttctat gtaagacatg caggtaggtg 180 gaca 184 <210> 127 <211> 184 <212> DNA <213> Sperm 8 Fin <400> 127 gccaatcttt gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttccccttct ccacactctc tcaccttctt aggaacctcc 120 agtagatcat ggttacttat aaaaatctgt gtttttctat gtaagacatg caggtaggtg 180 gaca 184 <210> 128 <211> 185 <212> DNA <213> Sperm 8 Minke <400> 128 gcaatcctct gtatttgttc atcaatagaa aaagggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttccccttct ccacactctc tcaccttctt aggaacctcc 120 agtagatcat ggctacttat aaaaatctag tgtttttcta tgtaagacat gcaggtaggt 180 ggaca 185 <210> 129 <211> 185 <212> DNA <213> Sperm 8 Hippo <400> 129 gccaatctct gtatttgttc atcaatagaa aacaggatgt gaagtattag ataaaaagat 60 ttctctaact tatctactca ttcccctcct tcacactttc tcaccttctc agtaacatcc 120 agtagatcat ggctacttat aaaaatctag tgtttttcta cgtaagacat gcaggtgggt 180 ggaca 185 <210> 130 <211> 468 <212> DNA <213> Sperm 28 Bottlenosed <220> <221> CD <222> (71) .. (365) <400> 130 gcttgtttag tggtggtgaa ctcttttagc ttttgcttat ctttaaaatt cttttttaaa 60 aattaattca ggacttccct ggtggtgcag tggttgagaa tctgcctgcc aatgcagggg 120 acacgggttc gagccctggt ctgggaagat cccacatgct gcggaggaac tgggcccatg 180 agccacagct actgagcctg cgcgtctgga gcctgtggtc tgcaacaaga gaggccatga 240 tagtgagagg cccgcacaac gggatgaaga gtggtccccg ctcaccgcaa ctagagaaag 300 ccctcgcaca gaaacgaaga cccaacacat ccaaaaataa ataaataaat taaaaaatta 360 attcaatcac tgataagtgt ttattgaata catattatgt gtcagacatt gtgttgggtg 420 cttgaaataa tggtgctaag cttggggata attattgctg tagcaggc 468 <210> 131 <211> 411 <212> DNA <213> Sperm 28 Amazon <220> <221> CD <222> (57) .. (353) <400> 131 tgtgacctct ttagcttttg cttaatctgt aaaattcttt ttttaaaatt aattcagggc 60 ttccctggtg gtgcagtggt tgagaatctg cctgccaatg caggggacac gggttcgagc 120 cctggtctgg gaagatccca catgccrcgg agcaactggg cccatgagcc acagctactg 180 agcctgcgtg tctggagcct gtgctccgca acaagagagg ctgtgatagt gagaggcctg 240 cgcaacggga tgaagagtgg tcccagctcg ccgcaactag agaaagccct caaacagaaa 300 ctaagaccca acacagccaa aaataaataa ataaatttaa aaaaattaat tcaatcactg 360 ttaagtgttt attgagtaca tattatatgt cagacattgt gtggggtgct a 411 <210> 132 <211> 414 <212> DNA <213> Sperm 28 Baiji <220> <221> CD <222> (58) .. (355) <400> 132 agagaacctc ttttagcttt tgcttatctt taaaattctt ttttaaaaat taattcaggc 60 cttccctggt ggtgcagtgg ttgagaatct gcctgccaat gcaggggaca cgggttcgag 120 gcctggtctg ggaagatccc acatgccgtg gagcaactgg gcccatgagc cacagctact 180 gagtctgcgt gtctggagcc tgtgctctgc aacaagagaa gccgtgatag tgagaggccc 240 gcgcaacggg atgaagagtg gtccctgctc gccacaacta gagaaagccc tcgcacagaa 300 acgaagaccc aacacagcca aaaataaata aataaattaa aaaaaaatta attcaatcag 360 tgataaatgt ttattgagta catattatgt gtcagacatt gtgttggggt gcta 414 <210> 133 <211> 470 <212> DNA <213> Sperm 28 Beaked <220> <221> CD <222> (71) .. (367) <400> 133 gcttgtttag tggtggtgaa ctcttttagc ttttgcttat ctgtaaaatt ctttttttta 60 aattaattca gggcttccgt ggtggtgcag tggttgagaa tctgcctgcc aatgcggggg 120 acacgggttc gagccctggt ctgggaagac cccacatgcc acggagcaac tgggcccatg 180 ggccacagct actgagcctg cgcatctgga gcctgtgctc tgcaacaaga gaggccgcga 240 tagtgagagg cccgcgcacc gcaatgaaga gtggtccccg ctcaccgcaa ctagagaaag 300 tgctcgcaca gaaacgaaga cccaacacag ccaaaaataa ataaattaat ttaaaaaaat 360 taattcaatc actgataagt gtttattgag tacatattat gtgtcagaca ttgtgttggg 420 tgcttgaaat aatggtgcta agcttgggga taattattgc tgtagcaggc 470 <210> 134 <211> 470 <212> DNA <213> Sperm 28 Sperm <220> <221> CD <222> (71) .. (368) <400> 134 gcttgtttag tggtggtgaa ctcttttagc ttttgcctat ctgtaaaatt ccttttttaa 60 aattaattca gggcttccct ggtggtgcag tggttgagaa tctgcctgcc aatgcagggg 120 acacaggttc aagccctggt ctgggaagat cccacatgcc gcagagcaac tgggcccgtg 180 agccacagct actgagcctg cacatctgga gcctgtgctc tgcaacaaga gaggccgcaa 240 tagtgagagg cccgcgcacc atgatgaaga gtggtccctg ctcaccgcaa ctagagaaag 300 ccctcgcaca gaaacgaaga tccaacacag ccaaaaataa ataaatacat ttttaaaaaa 360 ttaattcaat cactgataag tgtttactga gtacatatta tgtgtcagac attgtgttgg 420 gtgcttgaaa taatggtgtt aaacttggga taattattgc tgtagcaggc 470 <210> 135 <211> 172 <212> DNA <213> Sperm 28 Fin <400> 135 gcttgtttag tggtggtgaa ctcttttagc ttttgcttat ctgtaaaagt ttttttttta 60 attaattcaa tcactgataa gtgtttattg agtacatatt atgtggcaga cattgtgttg 120 ggtgcttgaa ataatggtgc taagcttggg gataattatt gctgtagcag gc 172 <210> 136 <211> 539 <212> DNA <213> Sperm 47 Bottlenosed <220> <221> CD <222> (137) .. (469) <400> 136 gggagaagga aatgaaaggc tttccctaaa actggacacc ccacccatta aggtgttaag 60 taattgcgtt cggtggggaa ctgctcagga tccaaggggg catcacgtgg aagccatgaa 120 tcaaatattt gcttaagggc ttccctggtg acgcagtggt tgagagtctg cctgccaatg 180 caggggacac gggttcgagc cctggtctgg gaggatccca catgccgcgg agcaactggg 240 cctgtgaacc ataactactg agcctgcgcg tctgaagcct gtaatccgca acaagagagg 300 ccgcgatagt gagaggcccg cgcaccgcga tggggagtgg cccccgctcg tcgcaactag 360 agaaagccct cgcacagaaa cgaaaaccca acgcagccat aaataaataa ataaatttta 420 aaaatgaaaa aaaaaggact acttaaaaaa tatatatata ttcgcttaag taagccaagg 480 gtctgagcgt ctaaatgacc ttcccaaagt catacaggaa catactggca cctccttca 539 <210> 137 <211> 530 <212> DNA <213> Sperm 47 Beaked <220> <221> CD <222> (137) .. (461) <400> 137 gggagaagga aatgaaaggc cttccctaaa actggacacc ccacccatta aggtgctaag 60 taattgagtt cggtggggaa ctgctcagga tccagggggg catcacgtgg aagccatgaa 120 tcaaatattc gcttaagggc ttccctggtg gcgcagtggt tgagaatctg cctgctagtg 180 caggggacac gggttcgagc cctggtctgg gaggatccca catgctgcgg agcaactagg 240 cccgtgagcc acgactactg agcctgcatg tctggagcct ctgctccgca acaggagagg 300 ccggcgatag tgagaggccc gcacgccgca atggggagtg gcccctgctt gccacaacta 360 gagaaaccct cgcacagaaa cgaagaccca acgcagccat aaataaattt ttaaaattaa 420 aaaaaagtac tttaaatata tatatatata tattcgctta agtaagccaa gggtctgacc 480 gtctaaatga cttcccaaag tcatacagga acatactggc acctccttca 530 <210> 138 <211> 525 <212> DNA <213> Sperm 47 Ganges <220> <221> CD <222> (103) .. (469) <400> 138 gacaccccac ccattaaggt gttaagtaat tgcgttcggt ggggaactgc tcaggatcca 60 ggggggcatc acgtggaagc catgaatcaa atattcactt cagggcttcc ctggtggcgt 120 agtggttgag aatctgcctg ccaatgcagg ggacacgggt tcgagcccta gtctgggaga 180 tccacatgtc gcagagcaac tgggccatga gccacactac tgagcctgcg catctggagc 240 ctgtactccg caacagagag gccgcgatag tgagaggccg cgcactacga tgaagagtgg 300 ccacgcttgc cacactagag aagccctcgc acagaaccga agacccaaca cagccataat 360 aataataata atttaaaatt aaaaaagact acttcaatat atatatatat atatatatat 420 atatattttt tttttttttt tttttttttt tttttttttt tttgcttaag taagccaagg 480 gtctgagcat ctaaatgact tcccaaagtc atacaggaac atact 525 <210> 139 <211> 544 <212> DNA <213> Sperm 47 Sperm <220> <221> CD <222> (136) .. (475) <400> 139 gggagaagga aatgaaaggc cttccctaaa actggacacc ccacccatta aggtgttaag 60 taattgcatt cggtggggaa ctgctcagga tccaaggggg catcagctgg aagccatgaa 120 tcaatattca cttaagggct tccctggtgg cacagtggtc gagaatctgc ctgccgatgc 180 aggggacacg ggttcgagcc ctggtctggg gagatcccac atgccgcgga gcaactgggc 240 ccttgagccg caactgctga gcctgtgcgt ctggagcctg tgctccgcag caagagaggc 300 tgcgatagtg agaggcccac gcaccgcgat gaagagcggc caccgcttgc cacaactaga 360 gaaagccctc gcacagaaac gaagacccaa cacagccata aagaaagaaa gaaagaaatt 420 ttaaaaatta aaaataaaag gactacttta aaaaaaaaga aaacaattcg cttaagtaag 480 ccaagggtct gagcgtctaa atgacttccc aaagtcatac aggaacatac tggcacctcc 540 ttca 544 <210> 140 <211> 205 <212> DNA <213> Sperm 47 Fin <400> 140 gggagaagga aatgaaaggc cttccctaaa actggatacc ccacccatta aggtgttacg 60 taattgtgtt cggtggggaa ctgctcagga tccaaggggg catcacgtgg aagccatgaa 120 tcaaatattc gcttaagtaa accaagggtc tgagcgtcta aatgacttcc caaagtcata 180 caggaacata ctggcacctc cttca 205 <210> 141 <211> 216 <212> DNA <213> Amz 13 Bottlenosed <400> 141 aggtccatca taacagaata cttgtctgcc ctcatgccaa cacgggacaa taaacttctt 60 gagaacaggc catttgcctt tcttctctct gtccctggca cctgacaaca aaaaagtgcc 120 caaataaacc tgcactttca gctacaggag ggtctgctgc aggagctact ggaacggcca 180 tgggggaact cagcaggagt catcagtggt ggagaa 216 <210> 142 <211> 442 <212> DNA <213> Amz 13 Amazon <220> <221> CDO <222> (122) .. (347) <223> "n" means undetermined. <400> 142 aggtccatca taacagaata cttgtctgcc ctcatgccaa cacagganaa taaacttctt 60 gagaacagac catttgcctt tcttctctct gtcccttgca cctgacaaca aaaaagtacc 120 cggcttgccc tggtggcgca gtggttgaga gccgcctgcc gatgcagggg acatgggttc 180 gtgctccggt ccgggaagat cccacatgcc gsrgcrgykg gcccgtgagc catggccgct 240 gagcctgcgc atccsgagym ygtgctccac aacgggagag gccacaacag tgagaggcct 300 gcttaccgca aaaaacagac aaaaaacaaa caaacaaaaa agtgcccaaa taaacctgtg 360 ttttcagcta caggagggtc tgttgcagga gctactggaa cggccatggg ggagctcagc 420 aggagtcatc agtggtggag aa 442 <210> 143 <211> 399 <212> DNA <213> Amz 13 La Plata <220> <221> CDO <222> (122) .. (356) <400> 143 aggtccatca taacagaata cttgtctgcc ctcatgccaa cacgggacaa taaacttctt 60 gagaacagac catttgcctt tcttctctct gtccctggca cctgacaaca aaaaagtgcc 120 cgggtttcac tggtggcgca gtggttgaga gtccgcctgc cgatgcaggg gacacgggtt 180 cgtgccccgg tccgggaaga tcccacatgc cgtggggcgt ctgggcccgt gagccatggc 240 tgctgagcct gtgcgtccgg agcctgtgct ccgcagcggg agaggccacg acagtgagag 300 gcccgcgtac cgcaaaaaca aaaaaacaaa cagaaaaaac agacaaaaaa gtgcccaaat 360 aaacctgtgt tttcagccac aggagggtct gttgcagga 399 <210> 144 <211> 105 <212> DNA <213> Amz 13 Baiji <400> 144 tgagaacaga ccatttgcct ttcttctctc tgtccctggc aactgacaac aaaaaagtgc 60 ccaaataaat ctgtgttttc agctacagga gggtctgttg cagga 105 <210> 145 <211> 217 <212> DNA <213> Amz 13 Humpback <400> 145 aggtccatca taacagaata cttgtctgcc ctcatgccaa cacgggacaa taaacttctt 60 gagaacagac catttgcctt tcttctctct gtccctggca cctgacaaca gaaaagtgcc 120 caaataaacc tgtgttttca actagaggag cggtctgctg caggagctac tggaacggcc 180 atgggggaac tcagcaggag tcatcagtgg tggagaa 217 <210> 146 <211> 184 <212> DNA <213> Amz 11 Bottlenosed <400> 146 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt ggagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacgtaagct gtgaaaagcc agaagcagaa 120 tgatgtcttt cccctgatgc aaaggtcact gctgtttttt tctcattcgt tccagctgac 180 caca 184 <210> 147 <211> 400 <212> DNA <213> Amz 11 Amazon <220> <221> CDO <222> (95) .. (309) <400> 147 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt atagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacggggctt ccctggtgcg cagtggttga 120 gagtccgcct gccgatgcag gggacacggg ctcgtgcccc gggccgggag gatcccacat 180 gctgcggaga ggctgggccc gtgagccatg gccgctgagc ctgtgcgtcc ggagcctgtg 240 ctctgcaacg ggagaggcac gatagtgaga ggccgcgtac cacaaaaaaa aaaaaaaaaa 300 ccacaaacgt aagctgtgaa aagccagaag cagagtgatg tctttcccct gatgcaaagg 360 tcactgcctg tttttttctc attcgttcca gctgaccaca 400 <210> 148 <211> 184 <212> DNA <213> Amz 11 La Plata <400> 148 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt agagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacgtaagct gtgaaaagcc agaagcagag 120 tgatgtcttt cccctgatgc aaaggtcact gctgtttttt tctcattcgt tccagctgac 180 caca 184 <210> 149 <211> 184 <212> DNA <213> Amz 11 Baiji <400> 149 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt aaagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacgtaagct gtgaaaagcc agaagcacag 120 tgatgtcttt cccctgatgc aaaggtcact gctgtttttt tctcattcgt tccagctgac 180 caca 184 <210> 150 <211> 184 <212> DNA <213> Amz 11 Sperm <400> 150 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt agagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacataagct gtgaaaagcc agaagcagaa 120 tgatgtcttt cccctgatgc aaaggtcact gctgtttttt tctcattcgt tccagctgac 180 caca 184 <210> 151 <211> 184 <212> DNA <213> Amz 11 Humpback <400> 151 gcatcatttg gctggttaga atttctgcca tttccctgtg gatcaagatt agagatgaac 60 tttcaggttc agtcatacat taaaaacaca aacataagct gtgaaaagcc agaagcagaa 120 tgatgtcttt cccctgatgc aaaggtcact gctgtttttt tctcattcgt tccagctgac 180 caca 184 <210> 152 <211> 579 <212> DNA <213> Tuti 24 Bottlenosed <400> 152 ctccttcagc aactccagtt acttgcacac taagccactt aacattgtcc cacagctcac 60 tgatactcta ttgtgcttta aagtctcttc tctttgtgct ccatcttgca tagtttcttg 120 tattgtgttc gagtttatta atcttttctc ctgcaacgta caatctgttg gtaataccat 180 tcagtatatt gtaaatttca aatgttggat atttcatttc tagaaatttg atttgggtct 240 tttttaatat tcttcatgtc taaaaattat tttttaaatc tttcttctag cttattgaac 300 atatgaaata acattataat atttagttta atgtctttgt ttactagttc catcatctgt 360 atcatttctg agacaatttt atttgactga ttttcctcct cattgtgaat tatatttttg 420 tagttctttg caagcccagt tggattttga gttactttat tataaataca ttacaacatt 480 acataaagtt tggggaaatt gaaaaagcaa aatgatttat aagctttcca tgctaataac 540 tttcattttt gttttctcat ctacacttca tccacatcc 579 <210> 153 <211> 819 <212> DNA <213> Tuti 24 Meso. Sp. <220> <221> CDO <222> (360) .. (595) <400> 153 ctccttcagc aactccagtt acttgcacac taagccactt aacattgtcc cacagctcac 60 tgatactcta ttgtgcttta aattctcttc tctttgtgct ccatcttgca tagtttcttg 120 tattgtgttc aggtttacta atcttttctc ctgcagtgta caatctgttg gtaataccat 180 tcagtatatt ttaaatttca aatgttggat atttcatttc tagaaatttg atttgggtct 240 ttttttaata ttcttcatgt ctaaaaatta tttttaaaat ctttcttcta gcttactaaa 300 catatgaaat aacattataa taattagttt aatgtctttg tttactagtt ccatcatctg 360 ggcttccctg gtggtgcagt ggttgagagt ttgcctgccg atgcagggca cacgggtttg 420 tgccccagtc agggaagatc ccacatgcca tggagtggct gggcccgtga gccatggcca 480 ctgagcctgt gtgtccggag cctgtgctcc ataacaggag aggccacaac agtgagaggc 540 ccacgtacca caaaaaaaca aacaaacaaa aatcaaacaa attagttcca tcatctgtat 600 catttctaag acacttttat ttgactgatt ttcctcttca ttgtgaatta tatttttgta 660 gttctttgca agcccagttg gattttgagt cactttatta taaatacatt acaacattac 720 ataaagtttg ggaaaattga aaaagcaaaa tgatttataa gctttccatg ttaataactc 780 tcatttttat gttttctcat ctacacttca tccacatcc 819 <210> 154 <211> 817 <212> DNA <213> Tuti 24 Beaked. Part. <220> <221> CDO <222> (362) .. (593) <223> "n" means undetermined. <400> 154 ctccttcagc aactccagtt acttgcatac taagccactt aacattgtcc cacagctcac 60 tgatactcta ttgtgcttta aattctcttt tctctttgtg ctccatcttg catagtttct 120 tgtattgtgt tcaagtttac taatcttttc tcctgcaatg tacaatctgt tggtaatacc 180 attaagtata ttttaaattt caagtgttgg atatttcatt tctagaaatt tgatttgggt 240 atttttttaa tattcttcat gtctaaaaat tattttttaa atctttcttc tagcttattg 300 aacatatgaa ataacattat aataattagt ttaatgtctt tgtttactag ttccatcatc 360 tgggcatccc tggtggtgca gtggttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gagcatggca 480 ctgagctgtg tgtctggagc tgtgctcata acaggagagg ccacaacagt gagaggcccg 540 cgtaccacaa aaaaaacaaa caaacaaaaa tcaaacaaat tagttccatc atctgtatca 600 tttctaagac acttttattt gactgatttt cctcctcatt gtgaattata tttttgtagt 660 tctttgcaag cccaattgga ttttgagtta ctttattata gatacattac aacattacat 720 aaagtttggg gaaattgaaa aagcaaaatg atttataagc tttccatgtt aataactttc 780 atttttatgt tttctcatct acacttcatc cacatcc 817 <210> 155 <211> 585 <212> DNA <213> Tuti 24 Sperm <400> 155 ctccttcagc aactccagtt acttgcacac taagccactt aacattgtcc cacagctcac 60 tgatactcta ttgtgcttta gagtctcttt tctctttgtg ttccatcttg catagtttct 120 tgtattgtgt tcaagtttac taatcttttc tcctgcgaga catacaatct gttggtaata 180 ccattcagta tattttaaat ttcaaatgtt ggatatttca tttctagaaa tttgattcgg 240 gtctttttta atattcttca tgtctaaaaa ttatttttta aatctttctt ctagcttatt 300 gatcatatga aataacatta taataattag tttaatgtct ttgtttacta gttccatcat 360 ctgtatcatt tctgagacac ttttatttga ctgattttcc tcctcattgt gaattatatt 420 tttgtagttc tttgcaagtc cagttggatt ttgagttact ttattataaa tacattacaa 480 cattacataa agtttgggga aattgaaaaa gcaaagtgat ttataagctt tccatgctaa 540 taactctcat ttttatgttt tctcatctac acttcatcca catcc 585 <210> 156 <211> 583 <212> DNA <213> Tuti 24 Humpback <400> 156 ctccttcagc aactccagtt acttgcacac taagccactt accattgtcc cacagctcac 60 tgatactcta ttgtgcttta aagtctcttt tctctttgtg ttccatcttg cataatttct 120 tgtattgtgt tcaagtttac taatcttttc tcctgcgaca tataatctgt tggtaatacc 180 attcagtata ttttaaattt caaatgttgg atatttcatt tctagaaatt tgatttgggt 240 cttttctaat attcttcatg tctaaaattt attttttaaa tctttcttct agcttattga 300 acatatgaaa taacattata ataattagtt taatgtcttt gtttactagt tccatcatct 360 gtatcacttc tgagacactt ttatttgact gattttcctc ctcattgtga attatatttt 420 tgtagttctt tgcaagccca gtcggatttt gagttacttt attataaata cattacaaca 480 ttacataaag tttggggaaa ttgaaaaagc aaaatgattt ataagctttc catgctaata 540 actttcattt ttatgttttc tcatctacac ttcatccaca tcc 583 <210> 157 <211> 162 <212> DNA <213> Tuti 35 Bottlenosed <400> 157 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctaa ttttaaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 158 <211> 162 <212> DNA <213> Tuti 35 Short-finned <400> 158 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctaa ttttaaaata 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 159 <211> 162 <212> DNA <213> Tuti 35 Dall's <400> 159 gccatactga ggcttaggca tgaattttgt tattttctgt ctgttgctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctaa ttttaaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 160 <211> 161 <212> DNA <213> Tuti 35 Narwhal <400> 160 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctaa ttttaaaatg 120 ttttttataa tatatgttac ttgccaacta tgtatgtagg g 161 <210> 161 <211> 158 <212> DNA <213> Tuti 35 Amazon <400> 161 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta tcaccaaaat 60 taatttgcta actcttacat aaaataatgt aacatttata gcctattttt aaaatgtttt 120 ttataagtat atgttacttg ccaactatgt atgtaggg 158 <210> 162 <211> 161 <212> DNA <213> Tuti 35 La Plata <400> 162 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaactctt acataacata atgtaacatt tatagtctat tttttaaatg 120 tttttataag tatatgttac ttgccaacta tgtatgtagg g 161 <210> 163 <211> 162 <212> DNA <213> Tuti 35 Baiji <400> 163 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctat ttttaaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 164 <211> 374 <212> DNA <213> Tuti 35 Beaked <220> <221> CDO <222> (111) .. (326) <400> 164 gccatactga ggcttaggca tgaattttgt tattttctgt ttcttaatta tcaccaagat 60 taatttgcta attcttacat aaaataatgt aacatttata gtctattttt tttttttttg 120 gcggtacgtg ggcctctcac tgttgtggcc tctcccattg cggagcacag gccccggaca 180 cgcaggctca gtggtcatgg ttcacagacc cagccactcc gcggcatgtg agatcctccc 240 ggaccggggc acgaacccgt gacccttgca tcagcaggca gactctcaac cactgcacca 300 ccagggaagc cctatagtct attttttaaa tgttttttat aagtatatgt tacttgccaa 360 ctatgtatgt aggg 374 <210> 165 <211> 375 <212> DNA <213> Tuti 35 Meso.sp. <220> <221> CDO <222> (111) .. (327) <400> 165 gccatactga ggcttaggca tgaattttgt tattttctgt ttcttaatta tcaccaaaag 60 taatttgcta attcttacat aaaataatgt aacatttata gtcttttttt tttttttttt 120 tgcggtacgc gggcctctca ctgttgtggc ctctcctgtt gtggagcaca ggctccggac 180 gcacaggctc agtggccatg gttcatgggc ctagccactc cgcagcatgt gagatcctcc 240 tggacagggg catgaaccca tgacccctgc atcggcaggc agactctcaa ccactgcacc 300 accagggaag ccctatagtc tattgttaaa atgtttttta taagtatatg ttacttgcca 360 actatgtatg taggg 375 <210> 166 <211> 163 <212> DNA <213> Tuti 35 Ganges <400> 166 gccatactga ggcttaggca tgaattttgt tattttctag tctgtttctt aattattacc 60 aaaattaatt tgctaattct tacataaaat aatgtaacat tcatagtcta tttttaaaat 120 gttttctgta agtatatgtt acttgccaac tatgtatgta ggg 163 <210> 167 <211> 162 <212> DNA <213> Tuti 35 Sperm <400> 167 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 gaattaattt gctaattctt acataaaata atgtaacatt tatagtctat ttttaaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 168 <211> 161 <212> DNA <213> Tuti 35 Humpback <400> 168 gccatactga ggttaggatg aattttgtta ttttctgtca tctttcttaa ttatcaccaa 60 aattaatttg ctaattctta cataaaataa cgtaacattt atagtctatt tttaaaatgt 120 tttttataag tatatgttac ttgccaacta tgtatgtagg g 161 <210> 169 <211> 162 <212> DNA <213> Tuti 35 Fin <400> 169 gccatactga ggcttaggca tgaattttgt tattatctgt ctgtttctta attatcacca 60 aaattaatct gctaattctt acataaaata atgtaacatt tatagtctat ttttaaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 170 <211> 162 <212> DNA <213> Tuti 35 Minke <400> 170 gccatactga ggcttaggca tgaattttgt tattttctgt ctgtttctta attatcacca 60 aaattaattt gctaattctt acataaaata atgtaacatt tatagtctat tttttaaatg 120 ttttttataa gtatatgtta cttgccaact atgtatgtag gg 162 <210> 171 <211> 160 <212> DNA <213> Tuti 35 Hippo <400> 171 gccatactga ggcttaggca tgaattttgt tattctgtct gtttcttaat tatcaccaaa 60 attaatttgc taattcttac ataaaataac gtaacattta tagtctattt tttaaatgtc 120 ttttataagt atacgttact tgccaactat gtatgtaggg 160 <210> 172 <211> 183 <212> DNA <213> Sp2 Bottlenosed <400> 172 gagataaatg aaggcaccca tttaaaatca ataggaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atacagctct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 173 <211> 183 <212> DNA <213> Sp2 Short-finned <400> 173 gagataaatg aaggcaccca tttaaaatca ataggaaagc atgaaaaaat aggcagaagt 60 gaaataagag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atatagctct 120 tgaaataaag gataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 174 <211> 185 <212> DNA <213> Sp2 Dall's <400> 174 gagataaatg aaggcaccca tttaaaatca gtaggaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaagctaa aagaaatgta ggaaacaaga atactagctc 120 ttgaaataaa gaataaactc aactaggata aaatataaac tgaaagtaga cagggaaaag 180 atcag 185 <210> 175 <211> 176 <212> DNA <213> Sp2 Narwhal <400> 175 gagataaatg aaggcaccca tttaaaatca gtaggaaagc atgaaaaaat aggcagaagt 60 gaaataagta atatgagaaa aaactaaaaa aaatgtagga aataagaata tagctcttga 120 aataataaac tcaacaggat aaaatataaa ctgaaagtag acagggaaaa gatcag 176 <210> 176 <211> 183 <212> DNA <213> Sp2 Amazon <400> 176 gagataaatg aaggcaccca tttaaaatca gtagaaaagc atgaaaaact aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga ttatagttct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 177 <211> 184 <212> DNA <213> Sp2 La Plata <400> 177 gagataaatg aaggcaccca tttagaatca gtaggaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atagagctct 120 tgaaataaag aataaactcc actaggataa aatataaact gaaagtagac agggaaaaga 180 tcag 184 <210> 178 <211> 184 <212> DNA <213> Sp 2 Baiji <400> 178 gagataaatg aaggcaccca tttaaaatca gtaggaaagc aggaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atatagctct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cagt 184 <210> 179 <211> 184 <212> DNA <213> Sp 2 Beaked <400> 179 gagataaatg aaggcaccca tttaaaatta ataggaaagc atgaaaaaat aggcaggagt 60 gaaataatag gtaatatgag aaaaaaacta aaagaaatct aggaaataag aatatagctc 120 ttgaaataaa gaataaactc aacaggataa aatataaact gaaagtagac agggaaaagg 180 tcag 184 <210> 180 <211> 183 <212> DNA <213> Sp 2 Ganges <400> 180 gagataaatg aaggcaccca tttaaaatca ataggaaagc atgaaaaaat aggcagaagt 60 gaagtaatag gtaatatgag aaaaaactaa atgaaatcta ggaaataaga atatagctct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 181 <211> 413 <212> DNA <213> Sp 2 Sperm <220> <221> CDO <222> (123) .. (351) <400> 181 gagataaatg aaggcaccca tttaaaatca ataggaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaaaata aaagaaatct aggaaataag aatatagctc 120 ttgggcttcc ctggtggcgc agtggttggg agtccgcctg ccgatgcagg ggacatgggt 180 tcgtgccccg gtctgggagg atcccacatg ccgcggagtg gctgggcctg tgagccatgg 240 ccgctgagcc tgcgcatccg gagcctgtgc tctgcaacgg gagaggccac aacagtgaga 300 ggcccgcgta ccgccaaaaa aaaaaaaaaa aaaaaataga atatagctct tgaaataaag 360 aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat cag 413 <210> 182 <211> 183 <212> DNA <213> Sp 2 Hump <400> 182 gagataaatg aaggcaccca tttagaatca ataagaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atatagctct 120 tgaaataaag aataaactca acagtataaa atacaaactg aaagtagaca gggaaaagat 180 cag 183 <210> 183 <211> 183 <212> DNA <213> Sp 2 Fin <400> 183 gagataaatg aaggcaccca tttaaaatca ataagaaagc atgaaaaaat aggcagaagt 60 gaaatagtag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atatagctct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 184 <211> 183 <212> DNA <213> Sp 2 Minke <400> 184 gagataaatg aaggcaccca tttaaaatca ataagaaagc atgaaaaaat aggcagaagt 60 gaaataatag gtaatatgag aaaaaactaa aagaaatcta ggaaataaga atatagctct 120 tgaaataaag aataaactca acaggataaa atataaactg aaagtagaca gggaaaagat 180 cag 183 <210> 185 <211> 183 <212> DNA <213> Sp 2 Hippo <400> 185 gagataaatg aaggcaccca tttaaaatca ataggaaaac atgaaaaaat aggcagaagt 60 gaaataatag gtagtatgaa aaagaactaa aagaaatcca ggaaataaga atatagcttt 120 tgaaacagaa tataactcaa caggataaaa tataaactga aagtagacag ggaaaagatc 180 agt 183 <210> 186 <211> 262 <212> DNA <213> Sp 9 Bottlenosed <400> 186 ccattttctg ggctcaacat aaatcttcca gactctagtg catttctagg gaagaacgga 60 gggaagccca gtaataactg ggattggagt tccttctact tcagtggaat taaggctgtg 120 atcatttaag tctgacgtga atttaatttg atttaaacac aataaagaaa cgtgacattt 180 cttgcacgcc caaatttgta cttacagctt taaacctaac tagtggtgga tactatatac 240 gaagcagtgt taaacgagat gt 262 <210> 187 <211> 262 <212> DNA <213> Sp 9 Amazon <400> 187 ccattttctg ggctcaacat aaatcttcca gaccctagtg catttctagg gaagaacgga 60 gggaagccca gtaataactg ggattggatt tccttctact tccgtggaat taaggctggg 120 atcatttaag tctgacgtga atttaatttg atttaaacac aataaagaaa cgtgacattt 180 cttgcacacc caaatttgta cttacagctt taaacctaac tagtggtgga tactataatc 240 gaagcagtgt taaacgagat gt 262 <210> 188 <211> 262 <212> DNA <213> Sp 9 Beaked <400> 188 ccattttctg ggctcaacat aaatcttcca gactctagtg catttctagg gaagaacgga 60 gggaagccca gtaataactg ggattggatt tccttctact tcagtggaat taaggctggg 120 ctcatttaag tctgaggtga atttaatttg atttaaacac gataaagaaa cgtgacattt 180 cttgcgcgcc caaatttgta cttacagctt taaacctaac tagtggtgga tactatatac 240 gaagcagtgt taaacgagat gt 262 <210> 189 <211> 486 <212> DNA <213> Sp 9 Sperm <220> <221> CDO <222> (178) .. (401) <400> 189 ccattttctg ggctcaacat aaatcttcca gactctagtg catttctagg gaagaacgga 60 gggaagccca gtaataactg ggattggatt tccttctact tcagtggaat taaggctggg 120 attatttaag tcggaggtga atgtaatttg atttaaacac aataaagaaa cgtgacaggg 180 cttacctggt ggcgcagtgg ttgagaatcc gcctgccgat gcaggggaca cgggttcgtg 240 ccccggtccg ggaagattcc cacatgccgc agagtggctg ggcccgtgag ccatggctgc 300 tgagcctscg catccggagc ctgtgctccg caacgggaga ggccacaaca gtgagaggcc 360 cgcgtaccgc aaaaaaaaaa aaaaaaaaaa gaaacgtgac atttcttgca tgcccaaatt 420 tgtacttaca gctttcaacc taactagtgg tggatactat atacgaagca gtgttaaacg 480 agatgt 486 <210> 190 <211> 262 <212> DNA <213> Sp 9 Humpback <400> 190 ccattttctg ggctcaacat aaatcttcca gactctagtg catttctagg gaggaatgaa 60 gggaagccca gtgataactg ggattggatt tccttctact tcagtgaaat taaggttggg 120 atcatttaag tctgaggtga atttaatttg atttaaacac aataaagaat cgtgacattt 180 cttgcacacc caaatttgta cttacagctt taaacctagc tagtggtgga tactatattc 240 gaagcagtgt taaacgagat gt 262 <210> 191 <211> 376 <212> DNA <213> Hump 20 Bottlenosed <400> 191 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgaga ttgcttactg 120 ccattctaca tgggtagaac agacgttggt gctcgcattt aagaactaga tcttccagag 180 aaggtcagca tagtgctctg ggaagcctcc agattcattt tagaggtcct aatgactctt 240 tttcatggtg ctttcaaatt actcatctca agcctatcat tatatacctc ttgttttcac 300 tggcggcatt ggagaccctc ttggcattat ttattgggct tcagtgatat caacaggagg 360 atccaatttc ccttca 376 <210> 192 <211> 376 <212> DNA <213> Hump 20 Short-finned <400> 192 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgaga ttgcttactg 120 ccattctaca tgggtagaac agacgttggt gcttgcattt aagaaataga tcttccagag 180 aaggtcagca tagtgctctg ggaagcctcc atattcattt tagaggtcct aatgactctt 240 tttcatggtg ctttcaaatt actcatctca agcctatcat tatatacctc ttgttttcac 300 tggcggcatt ggagtccctc ttggcattat ttattgggct tcagtggtat caacaggagg 360 atccaatttc ccttca 376 <210> 193 <211> 376 <212> DNA <213> Hump 20 Dall's <400> 193 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgaga ttgcttactg 120 ccattctaca tgggtagaac agacgttggt gcttgcattt aagaactaga tcttccagag 180 aaggtcagca tagtgctctg ggaagcctcc agattcattt tagaggtcct aatgactctt 240 tttcgtgatg ctttaaaatt actcatctca agcctatcat tatatacctc ttgttttcac 300 tggcggcatt ggaggccctc ttggcattat ttattgggct tcagtggtat caacaggagg 360 atccaatttc ccttca 376 <210> 194 <211> 376 <212> DNA <213> Hump 20 Narwhal <400> 194 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgaga ttgcttactg 120 ccattctaca tgggtagaac agacgttggt gcttgcattt aagaactaga tcttccagag 180 aaggtcagca tagtgctctg ggaagcctcc agattcattt tagaggtcct aatgactctt 240 tttcatggtg ctttaaaatt acttatctca agcctatcat tatatacttc ttgttttcac 300 tggcggcatt ggaggccctc ttggcattat ttattgggct tcagtggtat caacaggagg 360 atccaatttc ccttca 376 <210> 195 <211> 373 <212> DNA <213> Hump 20 Amazon <400> 195 atccacatat cagctggcat tacagtctgc caaagtatta acatgaatag agaaggaaat 60 gctttaacta gttttagctt taaaagttct ttaccacttt tatgtgattg cttactgcca 120 ttctacatgg gtagaacaga tgttggtgtt tgtttttaag aactagatct tccagagaag 180 gtcagcatag tgctctggga agcctccaga ttcattttag aggtcctaat gacttatttt 240 tgtggtgctt taaaattatt catctcaagc ctatcattat atacctcttg ttttcactgg 300 tggcattgga ggccctcttg gcattattta ttggtcttcg gtggtatcaa caggaggatc 360 caatttccct tca 373 <210> 196 <211> 376 <212> DNA <213> Hump 20 La Plata <400> 196 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagaggagga 60 aatgctttaa ctagttttag ctttaaaagt tcttcatcac ttttatgcga ttgcttactg 120 ccatcctaca cgggtagaac agatgttggt gtttgctttt aagaactaga tcttccagag 180 aaggtcagca gagtgctctg ggaagcctcc agattcattt tagaggtcct aatgactctt 240 tttcgtggtg ccttaaaatg agtcatctca agcctatcat tatatacctc ttgttttcac 300 tggcggcgct ggaggccctc tcggcattgt ttattggtct tcggtggtat caacaggagg 360 atccaatttc ccttca 376 <210> 197 <211> 373 <212> DNA <213> Hump 20 Baiji <400> 197 atccacatat cagctggcat tacagtctgc caaagtattc acatgaatag agaaggaaat 60 gctttaacta gttttagctt taaaagttct ttatcacttt tatgtgattg cttactgcca 120 ttctacatgg gtagaacaga tgttggtgtt tgcttttaag aactagatct tccagagaag 180 gtcagcatag tgctctggga agcctccaga ttcattttag aggtcctaat gactcttttt 240 cgtgttgctt taaaattatt catctcaagc ctatcattat atacctcttg ttttcaatgg 300 cggcattgga ggccctcttg gcattattta ttggtcttca gtggtatcaa caggaggatc 360 caatttccct tca 373 <210> 198 <211> 378 <212> DNA <213> Hump 20 Beaked <400> 198 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgtga ttgcttactg 120 ccattctgca tgggtagaac agatgttggt gtttgctttt aagaactaga tctcccagag 180 aaggtcagca ggtgctctgg gaagcctcca gattcatttt agagttccta atgactcttt 240 tttgtggtgc tttaaaatta ctcatctcaa gcttatcatt atatacctct tgttttcact 300 ggtggcattg gaggccctct tggcattatt tattgggctt cagtggtata cacaggagga 360 ggatccaatt tcccttca 378 <210> 199 <211> 365 <212> DNA <213> Hump 20 Ganges <400> 199 atccacatat cagctggcat tacagtgctg ccaaagtatt aacatgaata gagaaggaaa 60 tgctttaact agttttagct ttaactgttc tttatcactt ttatgtgatt gcttactgcc 120 attctacaga tgttggtgtt tgcttttaag aactagatct cccagagaag gccagcatag 180 tgctctggga agcctccaga ttcattttag aggtcctaat gactcttttt cgtggtgctt 240 taaaattact catctcaagc ctatcattat atactcttgt tttcactggt ggcattggag 300 gccctcttgg cattatttat tgggcttcag tggtatccac aggaggagga tccaatttcc 360 cttca 365 <210> 200 <211> 379 <212> DNA <213> Hump 20 Sperm <400> 200 cctatccaca tatcagctgg caatacagtc tgccaaagta ttaacatgaa gagagaagga 60 aatgctttaa ctagttttag ctttaaaagt tctttatcac ttttatgtga ttgcttactg 120 ccattctacg tgggtagaac agatgttggt gtttgctttt aagaactaga tctcccagag 180 aaggtcagca tagagctctg ggaagcctcc agattccttt tagaggtcct aatgactctt 240 ttttgtggtg ctttaaaatt actcatctca agcctatcat tgtatacctc ttgttttcac 300 tggcgtcatt ggaggccctc gtggcattat ttattgggct tcagtggtat caataggagg 360 aggatccaat ttcccttca 379 <210> 201 <211> 764 <212> DNA <213> Hump 20 Humpback <220> <221> CD <222> (177) .. (398) <400> 201 cctatccaca tatcagctgg cattacagtc tgccaaagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaactgt tctttatcag ttttatgtga ttgcttactg 120 ccattctaca tgggtagaac agatgttggt gtttgctttt aagaactaca tctcctgggc 180 ttccctggtg gcacagtggt tgagaatctg cctgccaatg caggggacat gggttcgagc 240 cctggtctgg gaagatccca catgccgcgg agcaactatg tccgtgagcc acaactactg 300 agcctgcgca tctggagcct gtgctccgca acaagagagg ccgcgatagt gagaggcccc 360 cgcaccgcga tgaagcgtgg cccccgcttg ccgcaactag agaaagcccc cgcacagaaa 420 cgaagaccca acacagccat aaataaataa ataaataaat aaataaataa cagtaggtct 480 ttcgataaat gaggggtcca agacaagaga accagaggtc cattggttta aaaaaaaaaa 540 aaaaaaaaaa ctagatctcc cagagaaggt cagcatagtg ctctgggaag cctccagatt 600 cattttagag gtcttaatga ctctttttca tgatgcttta aaattactca tctcaagcct 660 atcattatat acctcttgtt ttcactggag gcattggagg ccctcttggc attatttatt 720 gggcttcagt ggtatcaaaa ggaggaggat ccaatttccc ttca 764 <210> 202 <211> 762 <212> DNA <213> Hump 20 Fin <220> <221> CD <222> (177) .. (398) <400> 202 cctatccaca tatcagctgg cattacagtc tgtcagagta ttaacatgaa tagagaagga 60 aatgctttaa ctagttttag ctttaactgt tctttatcag ttttatgtga ttgcttactg 120 ccattctaca tgggtagaac agatgttggt gtttgctttt aagaactaga tctcctgggc 180 ttccctggtg gcacagtggt tgagaatctg cctgccaatg cagggggcat gggttcgagc 240 cctggtctgg gaagatccca catgccgcgg agcaactagg tccgtgagcc acaactactg 300 agcctgcgcg tctggagcct gcgctccgca acaagagagg ccgcaatggt gagaggccca 360 cgcatcgcga tgaagagtgg cccccagttg ccgcaactag agaaagccct tgcacagaaa 420 cgaagatcca acacagccat aaataaataa ataaataaat aaataaataa cagtaggtct 480 ttagataaat gaagggtcca agacaagaga accaggggtc cattggttta aaaaaaaaaa 540 acaaaaaact agatctccca gagaaggtca gcatagtgct ctgggaagcc tccagattca 600 ttttagaggt cttaatgact ctttttcgtg ctgctttaaa attactcatc tcaagcctat 660 cattatatac ctcttgtttt cactggcggc attggaggcc ctcttggcat tatttattgg 720 gcttcagtgg tatcaaaagg aggaggatcc aatttccctt ca 762 <210> 203 <211> 767 <212> DNA <213> Hump 20 Minke <220> <221> CD <222> (175) .. (397) <400> 203 tatccacata tcagctggca ttacagtctg ccaaagtatt aacatgaata gagaaggaaa 60 tgctttaact agttttagct ttaactggtc tttatcagtt ttatgtggtt gcttactgcc 120 attctacatg ggtagaacag atgttggtgt tttcttttaa gaactagatc tcctgggctt 180 ccctggtggc acagtggttg agaatctgcc tgccaatgca gggggcatgg gttcgagccc 240 tggtctggga agatcccaca tgccgcggag caattatgtc cgtgagccac aactactgag 300 cctgcgcgtc tggaccctgt gctccgcaac aagagaggcc gcgatagtga gaggccccgg 360 gcaccacgat gaagagcggc ccccacttgc cgcaactaga gaaagccctc gcacagaaac 420 gaagacccaa cacagccata aataaataaa taaataaata aataaataaa taaaaaacag 480 taggtctttt gataaatgag gggtccaaga caacagaacc agaggtccat tggtttaaaa 540 aaaaaacaaa aaactagatc tctcagagaa ggtcagcata gtgctctggg aagcctccag 600 atccatttta gaggtcttaa tgactctttt tcgtgatgct ttaaaattac tcatctcaag 660 cctatcatta tatacctctt gttttcactg gcggcattgg agaccctctt ggcattattt 720 attgggcttc agtggtatca acaggaggag gatccaattt cccttca 767 <210> 204 <211> 381 <212> DNA <213> Hump 20 Hippo <400> 204 cctatccaca tatcagctgg cattacagcc tgccaaaggg tttgaacatg aatagagaag 60 gaaatgcttt aactagttgt agctttaact gttctttacc acttttatgt gatcgcttgc 120 agccattcta caaggctaga acagatgttg gtgtctactt ctaagaacta gatctcccag 180 agaaggtcag catagtgccc tgggaagcct ccagattcat tttagaggtc ctaatgactc 240 tttttcatgg tgctttaaaa tcactcatct cagacctatc attatgtacc tcttgttttc 300 actggcggca ttggaggccc tcttggcatt atttatgggg ctttggtggt atcaacagga 360 ggaggatcca atttcccttc a 381 <210> 205 <211> 100 <212> DNA <213> Hump 203 Bottlenosed <400> 205 atacagtaca cttgggcagg aagttagtta ctatgtgtag tccaaactaa aatagtaggg 60 aattatgctc cacctccctg agggcagaat agcttcataa 100 <210> 206 <211> 100 <212> DNA <213> Hump 203 Amazon <400> 206 atacagtaca cttgggcagg aagttagtta ctatgtgtag tccaaactaa aatagtaggg 60 aattatgctc cacctccctg agggcagaat agcttcataa 100 <210> 207 <211> 100 <212> DNA <213> Hump 203 Beaked <400> 207 atacagtaca cttgggcagg aagttagtta ctatgtgtag tccaaactaa aatagtaggg 60 aattatgctc cacctccctg agggcagaat agcttcataa 100 <210> 208 <211> 100 <212> DNA <213> Hump 203 Sperm <400> 208 atacagtaca cttgggcagg aagttagtta ctctgtgtag tccaaactgg aatagtaggg 60 aattatgctc tacctccctg agggcagaat agcttcataa 100 <210> 209 <211> 403 <212> DNA <213> Hump 203 Humpback <220> <221> CD <222> (62) .. (364) <400> 209 atacagtaca cttgggcagg aagttagtta ctatgtgtag tccaaactaa aatagtaagg 60 ggggcttccc tggtggtgca gtggttgaga atctgcctgc caatgcaggg gacatgggtt 120 cgagccctgg tctgggaaga tcccacatgc cacggagcaa ctgggcccat gagccacaat 180 tactgagcct gcacgtctgg agcctgtgct ccgcaacaag agaggccatg atagtgagag 240 gcccgcgcac cacgatgaag agtggccccc gcttgccaca actagagaaa gctctcgcac 300 agaaacaaag accgaacaca gccaaaaata aataaataaa taaataaatt taaaatagta 360 gggaattatg ctctacctcc ctgagggcag aatagcttca taa 403 <210> 210 <211> 403 <212> DNA <213> Hump 203 Minke <220> <221> CD <222> (62) .. (364) <400> 210 atacagtaca cttgggcagg aagttagtta ctatgtgtag tccaaactaa aatagtaagg 60 ggggcttccc tggtggtgca gtggttgaga atctgcctgc caatgcaggg gacacgggtt 120 cgagccctgg tctgggaaga tcccacatgc cacggagcag ctaggcccgt gagccacaat 180 tactgagcct gcacgtctgg agcctgtgct ccgcaacagg aggggccatg atagtgagag 240 gcccgcgcac cgcgatgaag agtggccccc gcttgccgca actagagaaa gctctcgcac 300 agaaacgaag accgaacaca gccaaaaata aataaataaa taaataaatt taaaatagta 360 gggaattatg ctctacctcc ctgagggcag aatagcttca taa 403 <210> 211 <211> 390 <212> DNA <213> Mago 24 Bottlenosed <220> <221> CDO <222> (64) .. (289) <400> 211 caaactgaac aagaatgtgg attgagtggc cagcatacct cttcatttaa aacagggttt 60 ctcgggcttc cctggtggca cagtggttga gagtctgcct gctgatgcag gggacacggg 120 ttcgtgcccc ggtccaggaa gatcccacat gccgcggact gggcccgtga gccatggccg 180 ctgagcctgc gcgtccggag cctgtgctcc gcaacaggag aggccacaac agtgagaggc 240 ccgcgtaccg caaaaaaaaa aaaaagaaaa aacaaaagca gcgtttctca cctcaacagt 300 attgacatct tgagccagat gatgcttttt ttttttggac tggagactgg gggacaccac 360 tgcttctccc tcttagatgc ctccccagat 390 <210> 212 <211> 390 <212> DNA <213> Mago 24 Short-finned <220> <221> CDO <222> (65) .. (289) <400> 212 tcaaactgaa caagaatgtg gattgagtgg ccagcgtacc tcttcattta aaacagggtt 60 tctcgggctt ccctggtggc gcagtggttg agagtccgcc tgccgatgca ggggacacgg 120 gttcgtgccc cggtccggga agatcccaca tgccgcggac tgggcccgtg agccatggcc 180 gctgagcctg cgcgtccgga gcctgtgctc cgcaacggga gaggccacaa cagtgagagg 240 cccgcgtacc gcaaaaaaaa aaaaagaaaa aacaaaagca gcgtttctca cctcaacagt 300 attgacatct tgagccagat gatgcttttt ttttttggac tggagactgg gggacaccac 360 tgcttctccc tcttagatgc ctccccagat 390 <210> 213 <211> 396 <212> DNA <213> Mago 24 Dall's <220> <221> CDO <222> (65) .. (295) <400> 213 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagagct 60 tctcgggctt ccctggtggt gcagtggtta agagtctgcc tgccgatgca ggggacacgg 120 gttcgtgccc cggtctggga agatcccaca tgccgcggaa aggctgggcc catgagccat 180 ggccgctgag cctgcgcgtc cggagcctgt ctccgcaatg ggagaggcca caacggtgag 240 aggcccggta ccgcaaaaaa aaaaaaaaac acaaaaaaca aaaacagtgt ttctcacctc 300 aacagtactg acatcttgag ccagatgatg cttttgtttt ttggactgga gattggggga 360 caccactgct tctccctctt agatgcctcc ccagat 396 <210> 214 <211> 391 <212> DNA <213> Mago 24 Narwhal <220> <221> CDO <222> (65) .. (291) <400> 214 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagagtt 60 tctcgggctt ccctggtggc gcagtggttg agagtctgcc tgccgatgca ggggacacgg 120 gttcgtgccc cggtccagga agatcccaca tgccacggaa aggctgggcc cgtgagccat 180 ggccgctgag cctgcgcgtc cggagcctgt gctccgcaac gggagaggcc acaacagtga 240 gaggcccggt accgcaaaaa aaaaaaagaa aaaacaaaaa cagtgtttct cacctcaaca 300 gtactgacat cttgagccag atgatgcttt tttttttgga ctggagattg gtggacacca 360 ctgcttctcc ctcttagatg cctccccaga t 391 <210> 215 <211> 394 <212> DNA <213> Mago 24 Amazon <220> <221> CDO <222> (65) .. (293) <400> 215 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagggtt 60 tctcgggctt ccctggtggc gcagtggttg acagtccgcc tgtcaatgca ggggacacgg 120 gtttgtgccc cggtcctgga agatcccaca tgccgcggag cctgcgcccg tgagccatgg 180 ccgctgagcc tgtgcgtccg gagcctgtgc tccgcaacgg gagaggccac agtagtgaga 240 ggcctgcgta ccgcaaaaaa aaaaaaaaag aaaaaacaaa aacagggttt ctcacctcaa 300 cagtactgac atcttgagcc aggtgatgct tctttttttt ggactggaga ttgggggaca 360 ccactgcttc tccctcttag atgcctcccc agat 394 <210> 216 <211> 393 <212> DNA <213> Mago 24 La Plata <220> <221> CDO <222> (79) .. (292) <400> 216 tcaaactgaa caagaatgtg gattgagtgg ccgggtacct cttcaattaa aacagggttt 60 ctcgggcttc cccggtggcg cagtggttga gagtccgcct gccaatgcag gggaaagggt 120 ttgtgccccg gtccgggaag atcccacatg ccccggagcg gctgggcccg tgagccatgg 180 ccgctgagcc tgcacgtccg gagcctgtgc tctgcaacgg gatgaggcca caacagtgag 240 aggcccgcat accgcaaaaa aaaaaaaaga aaaaacaaaa acagcgtttc tcacctcaac 300 agtactgaca tcttgagcca gatgatgctt ttttgttttg gactggagat tgggggacac 360 cactgcttct ccctcttaga tgcctcccca gat 393 <210> 217 <211> 391 <212> DNA <213> Mago 24 Baiji <220> <221> CDO <222> (65) .. (289) <400> 217 tcaaactgaa caagaatgtg gattgagtgg ccagggaacc tcttcattta aaacagggtt 60 tctcgggctt ccctggtggc gcagtggttg agtgtccgcc tgccaatgca ggggacacgg 120 gttcgtgccc cggtccggga agatcccaca tgccgcggag cggctgggcc cgtgagccat 180 ggccgctgag cctgcgcgtc cggagcctgt gctccgcaac gggagaggcc acaacagtga 240 gaggcccgcg taccgcaaaa aaaaagaaaa aacaaaaaca gcgtttctca cctcaacagt 300 actgacatct tgagccagat gatgcttttt tttttttgga ctggagattg ggggacacca 360 ctgcttctcc ctcttagatg cctccccaga t 391 <210> 218 <211> 164 <212> DNA <213> Mago 24 Beaked <400> 218 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagggtt 60 tctcacctca acagtactga catcttgagc caggtgatgc tttgtttttt ggactggaga 120 ttgggggaca ccacagcttc tccctcttag atgcctcccc agat 164 <210> 219 <211> 166 <212> DNA <213> Mago 24 Ganges <400> 219 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagagtt 60 tctcacccca acagtactga catcttgagc cagatgatgc catttttttt ttggactgga 120 gattggggga caccactgct tctccctctt agatgcctcc ccagat 166 <210> 220 <211> 166 <212> DNA <213> Mago 24 Sperm <400> 220 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagtgtt 60 tctcacctca acagtactga catcttgagc cagatgatgc ttttttgttt ttggactgga 120 gattggggga caccactgct tctccctctt agatgcctcc ccagat 166 <210> 221 <211> 167 <212> DNA <213> Mago 24 Humpback <400> 221 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagcgtt 60 tctcacctca acagtactga catcttgagc cagatgatgc tttttttttt tttggacggg 120 agattggggg acaccactgc ttctccctct tagatgcctc cccagat 167 <210> 222 <211> 183 <212> DNA <213> Mago 24 Fin <400> 222 tcaaactgaa cagaatgtgg attgagtggc cagggtacct cttcatttaa aacagcgttt 60 ctcacctcaa cagtactgac atcttgagcc agatgatgct tttttttttt tttttttttt 120 tttttttttg gacgggagat tgggggacac cactgcttct ccctcttaga tgcctcccca 180 gat 183 <210> 223 <211> 172 <212> DNA <213> Mago 24 Minke <400> 223 tcaaactgaa caagaatgtg gattgagtgg ccagggtacc tcttcattta aaacagcgtt 60 tctcacctca acagtactga catcttgagc cagatgatgc tttttttttt tttttttggg 120 acgggagatt gggggacacc actgcttctc cctcttagat gcctccccag at 172 <210> 224 <211> 178 <212> DNA <213> Mago 24 Hippo <400> 224 tcaaactgaa caagaatgtg gattgagtgg ccagtgtatc ttttcattta aaaaagcatt 60 tcgtacctca atagtaccga catcttgagt cagacaaggt tttttgtttt ttttggtttt 120 ttttggactg gaaattgggg gataccactg cctctccctc ttagatgcct ccccagat 178 <210> 225 <211> 598 <212> DNA <213> Mago 26 Bottlenosed <220> <221> CDO <222> (99) .. (320) <400> 225 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaacg taacattaaa aagatctggg gcttccctgg tggtgcagta 120 gttgagagtc cgtctgccga tgcaggggac acgggttcgt gccccggtcc gggaggatcc 180 cacatgccat ggagcggctc tgggcccgtg agccatggcc actgggcctg tgcgtccaga 240 gcctgtgctc cgcaacggga gaggccgcaa cagtgagagg ctcacgaacc gaaaaaaaaa 300 aaaaaaaaaa aaaagatctg tattacgaat tccaaacaga gcacaaaaca agaaggaacg 360 accacaaaat tccaaatgta attaatatta gagtgctatt tctattagtt cagtaaatcc 420 tactctagtt gcaagtaaac gctgtcttct tcaaagcact ggtcccattg attggtttga 480 tcttggatct taaattgtca gtctcagctt tgatggccat attaaaatat ttttgctaga 540 agaaacatca cacttgcttt cacacattta cttggttcag gtgttgtctt gtgtaagg 598 <210> 226 <211> 591 <212> DNA <213> Mago 26 Short-finned <220> <221> CDO <222> (99) .. (314) <400> 226 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaaca taacattaaa aagatctggg gcttccctgg tggtgcagta 120 gttgagagtc cgcctgccgg tgcaggggac acaggatcgt gccctggtcc aggaggatcc 180 cacatgccac ggagcggctg ggcccgtgag ccatggccac tgggcctgtg cgtccagagc 240 ctgtgctccg caacgggaga ggccacaaca gtgagaggcc cacgaaccgc aaaaaaaaaa 300 aaaaaaaaga tctgtattac gaattccaaa cagagcacaa agcaagaagg aacgaccaca 360 aaattccaaa tgtaattaat attagagtgc tatttctatt agttcagtaa atcctactct 420 acttgcaagt aaacgctgtc ttcttcaaag cactggtccc attgattggt ttgatcttgg 480 atcttaaatt gtcagtctca gctttgatgg ccatattaag atatttttgc tagaagaaac 540 atcacacttg ctttcacaca tttacttggt tcaggtgttg tcttgtgtaa g 591 <210> 227 <211> 579 <212> DNA <213> Mago 26 Dall's <220> <221> CDO <222> (100) .. (307) <400> 227 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ctttaagtga 60 taaaaaataa tctgtgaaac gtaacattaa aaagatctgg ggcttccctg gtgacgcagt 120 agttgagagt ccgcctgctg atgcagggga cgtgggttcg tgccccggtc taggaagatc 180 ccacatgcca cagagcggct gggcccgtga gccatggccg ctgagcctgc ccgtccggac 240 cctgtgctcc gcaacgggag aggtcacaac agtgagaggc ccacaaactg caaaaaaaaa 300 agatctgtat tacgaattcc aaacagcaca aaaaaggaac gaccacaaaa ttccaaatgt 360 aattaatatt agagtgctat ttctattggt tcagtaaatc ctactctagt tgcaagaaaa 420 cgctgtcttc ttcaaaacac tggtcccatt gattggtttg atcttagatc ttaaattgtc 480 agtctcagct ttgatggcca tattaaaata tctctgctag aagacacatc acacttgctt 540 ttgcacattt acttggttca ggtgttgtct tgtgtaagg 579 <210> 228 <211> 594 <212> DNA <213> MAgo 26 Narwhal <220> <221> CDO <222> (99) .. (318) <400> 228 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttgaatga 60 taaaaataat ctgtgaaacg taacattaaa aagatctggg gcttccctgg tgacgcagta 120 gttgagagtc cgcctgccga tgcaggggac acgggttcgt gccccggtct gggaggatcc 180 cacatgccgc agagcggctg ggcccgtgag ccatggctgc tgagcctgcc cgtccggacc 240 ctgtgctccg caacaggaga ggtcacaaca gtgagaggcc cacagaccgc aaaaaaaaaa 300 aaaaaaaaaa aaaatctgta ttacggattc caaacagcac aaaacaaaaa ggaacgacca 360 caaaattcca aatgtaatta atattagagt gctatttcta ttggttcagt aaatcctact 420 ctagttgcaa gtaaacgctg tcttcttcaa agcactggtc ccattgattg gtttgatctt 480 agatcttaaa ttgtcagtct cagctttgat ggccatatta aaatatttct gctagaagaa 540 acatcacact tgcttttgca catttacttg gttcaggtgt tgtcttgtgt aagg 594 <210> 229 <211> 579 <212> DNA <213> Mago 26 Amazon <220> <221> CDO <222> (99) .. (314) <400> 229 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaacg taacattaaa aagatctggg gcttccctgg tggcgcagtg 120 gttgagagtc cgcctgccga tgcaggggat atgggttcgt gccccagtct gggaagatcc 180 cacatgccgc ggagtggctg ggcccgtgag ccatggccac tcggcctgcg tgtccggagc 240 ctgtgctccg caacgggaga ggccacaaca gtgagaggcc cacgaaccac aaaaaaaaac 300 cccaaaaaga tctgtattac gaattccaaa cagagcacaa aacaagaagg aatgaccaca 360 aaattccaaa tgtaattaat attagagtgc tatttatatt ggttcagtaa atcctactct 420 agttgcaagt aaacactgtc ttcttcaaag cactggtccc atcttggatc ttaaattgtc 480 agtctcagct ttgatggcta tattaaaata tttttgctag aagaaacatc acacttgctt 540 tcgcacattt acttggttca ggtgttgtct tgtgtaagg 579 <210> 230 <211> 590 <212> DNA <213> Mago 26 La Plata <220> <221> CDO <222> (99) .. (312) <400> 230 cacatacact ttgaaattac ttaccagtgt tttaaatata aattctcata ttttaaatga 60 taaaaataat ctgtgaaacg taacattaaa aagatctggg gcttccctgg tggcgcagta 120 gttgagagtc cgcctgccga tgcacgggac acaggttcgt gccccggtcc gggaggatcc 180 cacgttccat ggagcggctg ggcccttgag ccatggccac tgggcctgcg catccagagc 240 ctgtgctccg caacaggaga ggccacaaca gtgagaggcc cgtgaaccgc aaaaaaaaaa 300 ggaagagatc tgtattatga attccaaaca gagcacaaaa caagaaggaa tgaccacaaa 360 attccaaatg taattaatat tagagttcta tttctattag ttcagtaaat cctactctag 420 ttgcaagtaa atgctgtctt cttcaaagca ctggtcccat tgataggttt gatcttggat 480 cttaaattgt cagtctcagc tttgatgacc atattaaaat atttttgcta gaggaagcat 540 cacacttgct ttgacacatt tacttggttc aggtgttgtc ttgtgtaagg 590 <210> 231 <211> 605 <212> DNA <213> Mago 26 Baiji <220> <221> CDO <222> (99) .. (328) <400> 231 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaatg taacattaaa aagatctggg gcttccctgg tggcgcagta 120 ggtgagagtc cacctgccga tgcaggggac atgggttcgt gcccggtccg ggaggatccc 180 acatgccgca gagcggctgg gcccgtgagc catggccatt gggcctgcgc gtccagagcc 240 tgtgctccgc aacgggagag acccaacagt gagaggcccg cgtaccgcaa aaaaaaaaaa 300 aacaaaaaaa aacaacaaaa aaaatctgat tacgaattcc aaacagagca caaaacaaga 360 aggaacgacc acaaaattcc aaatgtaatt aatattagag tgctatttct actggttcag 420 taaatcctac tctagttgcg agtaaacgct gttttcttca aagcactagt cccattgatt 480 ggtttgatct tggatcttaa attgtcagtc tcagctttga tggccatatt aaaatatttt 540 tgctagaaga aacatcacac ttgctttcac acatttactt ggttcaggtg ttgtcttgtg 600 taagg 605 <210> 232 <211> 376 <212> DNA <213> Mago 26 Beaked <400> 232 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaact taacattaaa aagatctgta ttacgaattc caaacagagc 120 acaaaacaaa aaggaacgac cacaatattc caaatgtaat taatattaga gtgctatttc 180 tattggttga gtaaatccta ctctagttgc aagtaaacgc tgtcttcttc aaagtactgg 240 tccgattcat tggtttgttc ttggatctta aattgtcagt ctcagctttg atggccatat 300 taaaatattt ttgctagaag aaacatcaca cttgctttca cacatttact tggttcaggt 360 gttgtcttgt gtaagg 376 <210> 233 <211> 380 <212> DNA <213> Mago 26 Ganges <400> 233 cacatacact ttgaaattac ttaccagtat tttaagtata aattttcata tttttaatga 60 taaaaataat ctgtgaaact taacattaaa aagatctgta ttacgaactc caaacagagc 120 acaaaacaaa aaggaacgac cacaaaattc caaatgtaat taatattaga gtgctatttc 180 tattggttca gtaaattcct acactagttg caagtaaacg ctgtcttctt caaagcactg 240 gtcccattga ttggtttgtt cttggatctt aaattgtcag tctcagcttt gatggccata 300 ttaaaatatt tttgctagaa gaaacatcac acttgctttt gcactttcct tacttggttc 360 aggtgttgtc ttgtgtaagg 380 <210> 234 <211> 376 <212> DNA <213> Mago 26 Sperm <400> 234 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaacga 60 taaaaataat ctgtgaaact taacattaaa aagatctgta ttacaaattc caaacagagc 120 acaaaacaaa aaggaatgac caccaaattc caaatgtaat taatattaga gtgctatttc 180 tattggttca gtaaatccta ctctagttgc aagtaaacac tgtcttcttc aaagcactgg 240 tcccattgat tggtttgttc ttggatctta aattgtcagt ctcagctttg atggccatat 300 tgaaatattt ttgctagaag aaacatcaca cttgctttcg cacatttact tggttcaggt 360 gttgtcttgt gtaagg 376 <210> 235 <211> 376 <212> DNA <213> Mago 26 Humpback <400> 235 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaact taacattaaa aagatctgta ttacgaattc caaacagagc 120 acaaaacaaa aaggaacgac cacaaaattc caaatgtaat taatattaga gtgctatttc 180 tattggttca gtaaatccta ctctagttgc aagtaaacgc tgtcttcttc aaagcactgg 240 tcccattgat tggtttgttc ttggatctta aattgtcagt ctcagcattg atggccatat 300 taatatattt tttctagaag aaacatcaca cttgctttcg cacatttact tggttcaggt 360 gttgtcttgt gtaagg 376 <210> 236 <211> 366 <212> DNA <213> Mago 26 Fin <400> 236 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaact ttacattaaa aagatctgta ttacgaattc caaacagagc 120 acaaaacaaa aaggaacgac cacaaaattc caaatgtaat taatattaga gtgctatttc 180 tatgggttca gcaaatccta ctctagttgc tagtaaacgc tgtcttcttc aaagcactgg 240 tctcattgat tggtttgttc ttggatctta aattgtcagt ctcagctttg atggccatat 300 taaaatattt ttgctagaag aaacatcaca cttgctttcg cacatttact tggttcaggt 360 gttgtc 366 <210> 237 <211> 366 <212> DNA <213> Mago 26 Minke <400> 237 cacatacact ttgaaattac ttaccagtat tttaaatata aattttcata ttttaaatga 60 taaaaataat ctgtgaaact taacattaaa aagatctgta ttacgaattc caaacagagc 120 acaaaacaaa aaggaacgac cacaaaattc caaatgtaat taatattaga gtgctatttc 180 tattggttca gtaaatccta ctctagttgc aagtaaatgc tgtcttcttc aaagcactgg 240 tcccattgat tggtttgttc ttggatctta aattgtcagt ctcagcattg atggccatat 300 taatatattt tttgtagaag aaacatcaca cttgctttcg cacatttact tggttcaggt 360 gttgtc 366 <210> 238 <211> 372 <212> DNA <213> Mago 26 Hippo <400> 238 cacatacact ttgaaattac ttaccagtct tttaaatata attttcatat tttaaaagat 60 aaaaataatc tgtgaaattt aacattagaa agatctgtat tatgaaattc aaacagagca 120 aaaccaaaaa gcaacgacca cgaaattcca aatgtaatta ataataaggt gatacttcta 180 ttagttcagt aaatcctact cttgttgtca agtaaatgct ctcttcttca aagcactgat 240 cccattgatc agtttgttct tggatcttaa actgtcgatc tcagctttga tggccatatt 300 aaaatatttt tctagaaaac atcacacttg ctttcgcaca tttgcttggt tcaggtgttg 360 tcttgtgtaa gg 372 <210> 239 <211> 740 <212> DNA <213> Mago 22 Bottlenosed <220> <221> CDO <222> (349) .. (570) <400> 239 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata atgatgtgaa acaacagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatt aaatgtggat gttttatttt aaaagaaatt 240 tgaagtattt tcattttaga gtaaattatt tttctagatg aagagaaact atatgtggaa 300 aaatgcaaaa aaaaaaaagt ggcattttaa ttaagaataa agaaattagg gcttccctgg 360 tggtgcagtg gttgagagtc cgcctgccaa tgcaggggac acagtttcgt gccccggtcc 420 gggaagatcc cacatgccgc agagcggctg ggcccgtgag ccatggccac tgagcctgtg 480 catctggaac ctgtgctccg cagcgggaga ggccacaaca gtgagaggcc cgcgtaccac 540 aaaaaaaaaa agaaagaaag gaagaaatta gaacaaaaac caaatctgtt tgcatagatg 600 tgaagaagat tcacctataa ataatcctga aactaatttc tctagagact attttctggc 660 catttgagat caaggcacaa tctcaattga gactaagaat gacaacaaat atagataagt 720 tgtctcattg aacaggaacc 740 <210> 240 <211> 743 <212> DNA <213> Mago 22 Short-finned <220> <221> CDO <222> (352) .. (573) <400> 240 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaacagaaa atcctcaaat aaagagcaaa ttgtagtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatt aaatgtggat gttttatttt taaaagaaat 240 ttgaagtatt ttcattttag agtaaattat ttttctagat gaagagaaac tacatgtgga 300 caaatgcaaa aaaaaaaaaa agtggcattt taattaagaa taaagaaatt agggcttccc 360 tggtggtgca gtggttgaga gtccgcctgc cgatgcaggg gacacagttt cgtgccccgg 420 tccgggaaga tcccacatgc cgcagagcgg ctgggcccgt gagccatggc cactgagcct 480 gtgcatctgg aacctgtgct ccgcagcggg agaggccaca acagtgagag gcccgcgtac 540 cgcaaaaaaa gaaagaaaga aagaaagaaa ttagaacaaa aaccaaatct gtttgcatag 600 atgtgaagaa gattcaccta taaataatcc tgaaactaat ttctctagag actattttct 660 ggccatttga gatcaaggca caatctcaat tgagactaag aatgacaaca aatatagata 720 agttgtctca ttgaacagga acc 743 <210> 241 <211> 748 <212> DNA <213> Mago 22 Dall's <220> <221> CDO <222> (351) .. (575) <400> 241 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaaa gtggcatttt aattaagaat aaagaaatta gggcttccct 360 ggtggtgcag tggttgagag tccgcctgcc gatgcagggg atacgggttc gtgccccggt 420 ctgggaagat cccacatgcc gcagagcggc tgggcctgtg agccatggcc actgagcctg 480 tgtgtccaga acctgtgctc cacagtggga gaggtcacaa cagtgagagg cccgtgcacc 540 gcaaaaaaaa aagaaagaaa gaaagaaaga aattagaaca aaaaccaaat ctgtttgcat 600 agatgtgaag gagaagattc acctataaat aatactgaaa ctaatttttc tagagactat 660 tttctggcca tttgagatca aggcacaatc tcaattgaga ctaagaatga caacaaatat 720 agataagttg tctcattgaa caggaacc 748 <210> 242 <211> 752 <212> DNA <213> Mago 22 Narwhal <220> <221> CDO <222> (349) .. (579) <400> 242 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa attctcaaat aaagtagcaa attgtggtct 120 ttgttagaaa atgaaattat ttctatactt gttctgatta gttatttggt atgaactatt 180 gtttattatt tattataaaa ataaagagat ctaaatgtgg atgttttatt tttaaaagaa 240 atctgaagta ttttcatttt agagtaaatt atttttctag atgaagagaa actacatgtg 300 gaaaaatgca aaaaaaaagt ggcattttaa ttaagaataa agaaattagg gcttccctgg 360 tggtgcagtg gttgagagtc cgcctgccga tgcaggggac acgggttcgt gccctggtcc 420 gggaagatcc cacatgccgc gaagcggctg ggcctgtgag ccatggccac tgagcctgtg 480 cgtccagaac ctgtgctccg cagtgggaga ggccacaaca gtgagaggcc cgcgtaccgc 540 aaaaaaaaaa aagaaagaaa gaaagaaaga aagaaattag aacaaaaacc aaatctgtgt 600 gcatagatgt gaaggagaag attcacctat aaataatcct gaaactaatt tttctagaga 660 ctattttctg gccatttgag atcaaggcac aatctcaatt gagactaaga atgacaacaa 720 atatagataa gttgtctcat tgaacaggaa cc 752 <210> 243 <211> 734 <212> DNA <213> MAgo 22 Amazon <220> <221> CDO <222> (337) .. (561) <400> 243 gcccactaaa tactactaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgca acaatagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctgtacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctacg tgtggaaaaa tgcaaaaaaa 300 aaaaaagtgg cattttaatt aagaataaag aaattagggc ttccttggtg gtgcagtggt 360 tgagagtcca cctgccgatg caggggacac gggtttgtgc cccggtccgg gaagatccca 420 cgtgccgcag agcggctggg cccgtgagcc atggccgctg agcctgcgcg tccagagatt 480 gtgctccgca acgggagagg ccacaacagt gagaggccca cgtacagcaa aaaaaaaaaa 540 aaaaaaagaa taaagaaatt agaacaaaag ccaaatctgt ttgcatagat gtgaaggaga 600 agattcacct ataaataatc ctgaaactaa tttctctaga gactattttc tggccatttg 660 agatcaaggc acaatctcaa ttgagactaa gaatgacaac aaatatagat aagttgtctc 720 attgaacagg aacc 734 <210> 244 <211> 739 <212> DNA <213> Mago 22 La Plata <220> <221> CDO <222> (350) .. (566) <400> 244 gcccactaaa tactattaca ctaaagcaat tagacttaac taagcctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa atcctcaaat aaagagcaga ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagttt taaatctgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtag 300 aaaaatgcaa aaaaaaaaag tagcgtttta attaagaata aagaaattag ggcttccctg 360 gtggtgcagt ggttgagagt ccacctgccg aagcagggga cacgggttcg tgccccaatc 420 cgggaggatc ccacatgcca cggagcggct gggcccatgt gccgtggccg ctgagcctgc 480 gcgtccagag actgtgctct gcaacaggag aggccacaac agtgagaggc ccacgtaccg 540 caaaaaaaaa aagtataaag agattagaac aaaaaccaaa tctgtttgca tagatgtgaa 600 ggaaaagatt cacctataaa taatcgtgaa actaatttct ctagagacta ttttctggcc 660 atttgagatc aaggcacaat ctcaattgag actaagaatg acaacaaata tagataagtt 720 gtctcattga acaggaacc 739 <210> 245 <211> 734 <212> DNA <213> Mago 22 Baiji <220> <221> CDO <222> (344) .. (561) <400> 245 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttagta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta cagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaaa gtggcatttt aataaagaaa ttagggcttc cctggtgatg 360 cggtggttga gagtccgcct gccgatgcag gggacgcggg ttcgggcccc ggtccgggaa 420 gatcccacat gccgcggagc ggctgggcct gtgagtcatg gctactgagc ccgtgcgtcc 480 agaacctgtg ctctgcagcg ggagaggcca caacagtgag aggcccgcgt accgcaaaaa 540 aaaaaaagaa taaagaaatt agaacaaaaa ccaaatctgt ttgcatagac gtgaaggaga 600 agattcacct ataaataatc ctgaaactaa tttctctaga gactgttttc tggccatttg 660 agatcaaggc acaatctcaa ttgagactaa gaatgtcaac aaatatagat aagttgtctc 720 attgaacagg aacc 734 <210> 246 <211> 740 <212> DNA <213> Mago 22 Beaked <220> <221> CDO <222> (349) .. (567) <400> 246 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa accctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgatattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaagt ggcattttaa ttaagaataa agaaatcagg gcttccctgg 360 tggcacagtg tttgagagtc cacctgccga tgcaggggac acgggtttgt gccccggtcc 420 gggaagatcc cacatgcctc ggagcggcta ggcccatgag ccatggccac tgagcctgtg 480 cgtccggaac ctgtgctccg caatgggaga ggccacaaca gtgagaggcc cacgtactgc 540 aaaaaaaaaa aaagaataaa gaaattagaa caaaaaccaa atctgtttgc atagatgtga 600 aggagaagat tcacctataa ataatcctga aactaatttc tctagagact attttctggc 660 catttgagat caaggcacaa tctcaattga gactaagaat gacaacgaat atcgataagt 720 tgtctcattg aacaggaacc 740 <210> 247 <211> 752 <212> DNA <213> Mago 22 Ganges <220> <221> CDO <222> (352) .. (579) <400> 247 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa gcagatgata 60 cgttagaata attatgtgaa acagtagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg tgctgattgg ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttca gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaaa agtggcattt taattaagaa taaagaaatt agggcttccc 360 tggtggtaca gtggttgaga gtctgcctgc cgatgcaggg gacgcgggtt cgtgccccgg 420 tccgggaaga tcccacaagc cgcagagcgg ctaggcccgt gagccatggc cgctgagcct 480 gcgcatctgc agcctgtgct ccacaacggg agaggccaca acagtgagaa tcccgcatac 540 ggcaaaaaaa aaaaaaaaaa aaaaagaata aagaaattgg aacaaaaacc aaatctgttt 600 gcacagatgt gaaggagaag attcacctat aaataatcct gaaactaatt tctctagaga 660 ctattttctg gccatttgag atcaaggcac aatctcaatt gagactaaga atgacaacaa 720 atatagataa gttgtctcat tgaacaggaa cc 752 <210> 248 <211> 523 <212> DNA <213> Mago 22 Sperm <400> 248 gcccactaaa tactattaca ctaaagcaat tagacttagc taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa atcctcaaat aaagagcaaa ttgtggtctt 120 tgttagaaaa tgaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattt attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaaa gtggcatttt aattaagaat aaagaaatta gaacaaaaac 360 caaatctgtt tgcatagatg tgaaggagaa gattcaccta taaataatcc tgaaactaat 420 ttctctagag actattttct ggccatttgt gctcaaggca caatctcaat tgagactaag 480 aatgacaacg aatatagata agttgtctca ttgaacagga acc 523 <210> 249 <211> 525 <212> DNA <213> Mago 22 Humpback <400> 249 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtccaaa atagatgata 60 cattagaata attatgtgaa acagtagaaa atcctcaaat aaagaacaaa ttgtggtctt 120 tgttagaaaa agaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattc attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaaa aagtggcatt ttaattaaga ataaagaaat tattacaaaa 360 gccaaatctg tttgcataga tgtgaaggag aagattcacc cataaataat cctgaaacta 420 atttctctag agactatttt ctggccattt gagatcaagg cacaatctca attgagacta 480 agaatgacaa cgaatataga taagttgtct cattgaacag gaacc 525 <210> 250 <211> 523 <212> DNA <213> Mago 22 Fin <400> 250 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acaatagaaa atcctcaagt aaagaacaaa ttgtggtctt 120 tgttagaaaa agaaattatt tccatacttg ttctgattag ttatttggta tgaactattg 180 tttattattc attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcacttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaagaaaaaa gtggcatttt aattaagaat aaagaaatta ttacaaaagc 360 caaatctgtt tgcatagatg tgaaggagaa gattcaccta taaataatcc tgaaactaat 420 ttctctagag actattttct ggccatttga gatcaaggca caatctcagt tgagactaag 480 aatgacaacg aatatagata agttgtctca ttgaacagga acc 523 <210> 251 <211> 522 <212> DNA <213> Mago 22 Minke <400> 251 gcccactaaa tactattaca ctaaagcaat tagacttaac taagtctaaa atagatgata 60 cattagaata attatgtgaa acagtagaaa atcctcaaat aaagaacaaa ttgtggtctt 120 tgttagaaaa agaaattatt tctatacttg ttctgattag ttatttggta tgaactattg 180 tttattattc attataaaaa taaagagatc taaatgtgga tgttttattt ttaaaagaaa 240 tttgaagtat tttcatttta gagtaaatta tttttctaga tgaagagaaa ctacatgtgg 300 aaaaatgcaa aaaaaaaaag tggcatttta actaagaata aagaaattat tacaaaagcc 360 aaatctgttt gcatagatgt gaaggagaag attcacctat aaataatcct gaaactaatt 420 tctctagaga ctattttctg gccatttgag atcaaagcac aatctcaatt gagactaaga 480 atgacaacga atatagataa gttgtctcat tgaacaggaa cc 522 <210> 252 <211> 518 <212> DNA <213> Mago 22 Hippo <400> 252 gcccactaaa tactattaca ctaaagcaac tagacttaac ttaggtaagt ctacaataga 60 taatacatta gaagaattca gtgaaacaat agaagatctt caaataaaga gcgtattgca 120 gtctttgtta gaaaatgaaa ttatctctgt acttgctcag gttagttatt tggtatgaac 180 tatggcttat tacttattat aaaaataaaa gaatctaaat gttggtgttt tatttttaga 240 agaaatttaa agcattttca ttttagagta aatgattttt ctagaggaaa agaaactaca 300 tgtgaaaaat gcaaaaaaag aaagcggcat tttaataaaa tataaagaaa ttggaacaaa 360 ttgtttgcat gaatatgaag gagaagattc acctataaat aatcttgaaa ttaatttctc 420 tagagactat tttctggcca tttgagatca aggcacaatc tcaattgaga ctaagaatga 480 caactaatat agataagttg tctcattgaa caggaacc 518 <210> 253 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHRS F Oligonucleotide for PCR <400> 253 gtggtctagt ggttaggayy yrg 23 <210> 254 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR R oligonucleotide for PCR <400> 254 cagttcccag accagggatt g 21 <210> 255 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type I CHR-1 F oligonucleotide for PCR <400> 255 gtggcacagt ggttaagaat ctg 23 <210> 256 <211> 19 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type I CHR-1 R oligonucleotide for PCR <400> 256 ctgcacagct tgtgggatc 19 <210> 257 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type II Type II Fologonucleotide for PCR and sequencing <400> 257 gcrgtccagt ggttaagact 20 <210> 258 <211> 21 <212> DNA <213> Artificial Sedquence <220> <221> misc_feature <223> CHR-1 Type II Type II R oligonucleotide for PCR and sequencing <400> 258 rcagcatgtg ggatcttagt t 21 <210> 259 <211> 26 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type II Type II Probe <400> 259 taagactctg hgcttccamt gcaggg 26 <210> 260 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type III Type III Probe <400> 260 aatgcagggg gcccrggttt ga 22 <210> 261 <211> 18 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type III Type III F oligonucleotide for PCR and sequencing <400> 261 gacttccctg gtggtcca 18 <210> 262 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type III Type III R oligonucleotide for PCR <400> 262 ctgaccaggg atcaaacccg 20 <210> 263 <211> 19 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-1 Type III Type III R SEQ oligonucleotide for sequencing <400> 263 cctgaccagg gatcaaacc 19 <210> 264 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 Full length CHR-2 F oligonucleotide for PCR <400> 264 gtggcacagt ggttaagaat ctg 23 <210> 265 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 Full length CHR-2 R oligonucleotide for PCR <400> 265 tgcgttgggt ctttgttgct 20 <210> 266 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily CD probe <400> 266 tctggagcct gtgctccgca 20 <210> 267 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily CD probe 2 <400> 267 caagagaggc cgcgatagtg a 21 <210> 268 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily CD prb2'R oligonucleotide for PCR <400> 268 gcctctcact atcgcggcct 20 <210> 269 <211> 20 <212> DNA <213> Artificial Sequuence <220> <221> misc_feature <223> CHR-2 CD subfamily M. Up oligonucleotide for PCR and sequencing <400> 269 ccctggtccg ggaagatccc 20 <210> 270 <211> 19 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily M. Down oligonucleotided for PCR and sequencing <400> 270 ggctgtgttg ggtcttcgt 19 <210> 271 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily # 13 Up oligonucleotide for sequencing <400> 271 ctggtccggg aagatcccac 20 <210> 272 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CD subfamily # 13 Up (Anti) oligonucleotide for sequencing <400> 272 gtgggatctt cccggaccag 20 <210> 273 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CDO subfamily Bera-1 probe <400> 273 cgcaayrgga gaggccacaa 20 <210> 274 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CDO subfamily CDO F1 oligonucleotide for PCR and sequencing <400> 274 gcagtggttg agagtchgcc tg 22 <210> 275 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> CHR-2 CDO subfamily CDO R2 oligonucleotide for PCR and sequencing <400> 275 ttgtggcctc tccyrttgcg 20 <210> 276 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Bando 1 Bando F1 PCR primer <400> 276 ttgtcaaggt gcttcgcttt ag 22 <210> 277 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Bando 1 Bando 1 R1 PCR primer <400> 277 gcccccagac tctgttttaa tag 23 <210> 278 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp316 Sp316 F1 PCR primer <400> 278 tcctccagtg tggcttcata 20 <210> 279 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp316 Sp316 R1 PCR primer <400> 279 ttactgcatg gggtttagtc aa 22 <210> 280 <211> 25 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm8 Sperm8 F1 PCR primer <400> 280 gccaatctct gtatttgttc atcaa 25 <210> 281 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm8 Sperm8 R1 PCR primer <400> 281 gtccacctac ctgcatgtct tac 23 <210> 282 <211> 27 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm28 Sperm28 F1 <400> 282 gtgaactctt ttagcttttg cttatct 27 <210> 283 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm28 Sperm28 R1 PCR primer <400> 283 agcacccaac acaatgtctg 20 <210> 284 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm47 Sperm47 F1 PCR primer <400> 284 gacaccccac ccattaaggt 20 <210> 285 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm47 Sperm47 F2 PCR primer <400> 285 gggagaagga aatgaaaggc 20 <210> 286 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm47 Sperm47 R1 PCR primer <400> 286 tgaaggaggt gccagtatgt t 21 <210> 287 <211> 25 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sperm47 Sperm47 R2 PCR primer <400> 287 agtatgttcc tgtatgactt tggga 25 <210> 288 <211> 24 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago21 Mago21 F2 PCR primer <400> 288 ccttcttcat caggttatta ggaa 24 <210> 289 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago21 Mago21 R2 PCR primer <400> 289 ggaaatcaac aaatcccact a 21 <210> 290 <211> 27 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago22 Mago22 F1 PCR primer <400> 290 gcccactaaa tactattaca ctaaagc 27 <210> 291 <211> 27 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago22 Mago22 R1 PCR primer <400> 291 ggttcctgtt caatgagaca acttatc 27 <210> 292 <211> 24 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago8 Mago8 F2 PCR primer <400> 292 gctaactcta gattgcaatg aacc 24 <210> 293 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago8 Mago8 R2 PCT primer <400> 293 gggaattttt cgtgattgag c 21 <210> 294 <211> 28 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mgo13 Mago13 F3 PCR primer <400> 294 aaaaaatgtt tctatcacta tctacaat 28 <210> 295 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago13 Mago13 R2 PCR primer <400> 295 ctgggctgta cttttgctgg 20 <210> 296 <211> 24 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago24 Mago24 F1 PCR primer <400> 296 tcaaactgaa caagaatgtg gatt 24 <210> 297 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago24 Mago24 R1 PCR primer <400> 297 atctggggag gcatctaaga g 21 <210> 298 <211> 28 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago26 Mago26 F3 PCR primer <400> 298 cacatacact ttgaaattac ttaccagt 28 <210> 299 <211> 24 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago26 Mago26 R2 PCR primer <400> 299 ccttacacaa gacaacacct gaac 24 <210> 300 <211> 26 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago32 Mago32 F2 PCR primer <400> 300 atagtttggt acaatttcat tcctac 26 <210> 301 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Mago32 Mago32 R2 PCR primer <400> 301 agaaatagct catgtgttgt cct 23 <210> 302 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi14 Isi14 F1 PCR primer <400> 302 ttcccctata ttctccatgg ttt 23 <210> 303 <211> 28 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi14 Isi14 R2 PCR primer <400> 303 ggtcattatt actcattaag atatgtgg 28 <210> 304 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi36 Isi36 F2 PCR primer <400> 304 aaacccattt taactgcaga gt 22 <210> 305 <211> 25 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi36 Isi36 R2 PCR primer <400> 305 gtaacaattc atatacttgg gaagg 25 <210> 306 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi38 Isi38 F1 PCR primer <400> 306 ttgaatcata agctctcgtc cat 23 <210> 307 <211> 24 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Isi38 Isi38 R1 PCR primer <400> 307 ataaacacat gaaagtccag attg 24 <210> 308 <211> 21 <212> DNA <213> Artificial Sequenc <220> <221> misc_feature <223> Mago19 Mago19 F1 PCR primer <400> 308 ctgcacagtt ttggctcaat c 21 <210> 309 <211> 23 <212> DNA <213> Artificial Sequnce <220> <221> misc_feature <223> Mago19 Mago19 R1 PCR primer <400> 309 ctggtcatgt gtaagcatca ctt 23 <210> 310 <211> 26 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Amz13 Amz13 F1 PCR primer <400> 310 aggtccatca taacagaata cttgtc 26 <210> 311 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Amz13 Amz13 R1 PCR primer <400> 311 ttctccacca ctgatgactc c 21 <210> 312 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Amz11 Amz11 F1 PCR primer <400> 312 gcatcatttg gctggttaga at 22 <210> 313 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Amz11 Amz11 R1 PCR primer <400> 313 tgtggtcagc tggaacgaat 20 <210> 314 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Tuti24 Tuti24 F2 PCR primer <400> 314 ctccttcagc aactccagtt ac 22 <210> 315 <211> 22 <212> DNA <213> Artificial Sequenced <220> <221> misc_feature <223> Tuti24 Tuti24 R2 PCR primer <400> 3150 ggatgtggat gaagtgtaga tg 22 <210> 316 <211> 26 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Tuti35 Tuti35 F1 PCR primer <400> 316 ccctacatac atagttggca agtaac 26 <210> 317 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Tuti35 Tuti35 R1 PCR primer <400> 317 gccatactga ggcttaggca t 21 <210> 318 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp9 Sp9 F1 PCR primer <400> 318 ccattttctg ggctcaacat aa 22 <210> 319 <211> 23 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp9 Sp9 R1 PCR primer <400> 319 acatctcgtt taacactgct tcg 23 <210> 320 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp2 Sp2 F1 PCR primer <400> 320 gagataaatg aaggcaccca t 21 <210> 321 <211> 26 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Sp2 Sp2 F1 PCR primer <400> 321 tactgatctt ttccctgtct actttc 26 <210> 322 <211> 21 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Hump20 Hump20 up PCR primer <400> 322 cctatccaca tatcagctgg c 21 <210> 323 <211> 20 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Hump20 Hump20 down PCR primer <400> 323 tgaagggaaa ttggatcctc 20 <210> 324 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Hump203 Hump203 F1 PCR primer <400> 324 atacagtaca cttgggcagg aa 22 <210> 325 <211> 22 <212> DNA <213> Artificial Sequence <220> <221> misc_feature <223> Hump203 Hump203 R1 PCR primer <400> 325 ttatgaagct attctgccct ca 22

[Brief description of drawings]

1 is a general diagram of the SINE retro position.

FIG. 2 (A) represents the master gene model and FIG. 2 (B) represents the multisource gene model.

FIG. 3 shows the utility of SINE as an indicator of evolution.

FIG. 4 represents a phylogenetic tree model of species AD. Figure 4 (C) shows P
Indicates a CR pattern.

FIG. 5 is an example of some patterns of transcripts from total genomic DNA from selected animal species.

FIG. 6 shows the tRNA-like structure of CHR-2 SINE.

FIG. 7 (A) is a tRNA of CHR-2 SINE.
FIG. 7 (B) shows human tRNA.
The secondary structure of Glu is shown.

FIG. 8 depicts the construction of the consensus sequence for CHR-2 SINE from several sequence alignments of CHR-2.

FIG. 9: A phylogenetic tree containing old SINEs amplified at t and young SINEs amplified at u.

FIG. 10 represents the identification of the characteristic nucleotides or possible deletions representing a subfamily of the SINE family by alignment.

FIG. 11 shows an alignment of consensus sequences for the CHR-2 SINE family subfamily.

FIG. 12 shows the results of dot-hybridization experiments using probes specific for CD, CDO, and other subfamilies, respectively.

FIG. 13 schematically shows the principle of flanking SINE PCR.

FIG. 14 shows an example of a flanking PCR result. Further, FIGS. 14B and 14C simultaneously show the results of the hybridization experiment conducted using the same filter using two different probes.

FIG. 15 shows a phylogenetic tree of a mammal.

FIG. 16 shows the flow of experiments in the SINE method.

FIG. 17 shows a phylogenetic tree advocated from molecular statistical studies (a controversy over the problem of monophyly of whales).

FIG. 18 shows an alignment of consensus sequences for each CHR-2 subfamily.

FIG. 19 shows band patterns of Bando1 and Sp316 loci showing that SINE was commonly inserted in Whale eyes.

FIG. 20 shows the results of alignment of the locus Bando1.

FIG. 21 shows the results of alignment of the locus Bando1.

FIG. 22 shows the results of alignment of the Bando1 locus.

FIG. 23 shows the results of the alignment of the locus Sp316.

FIG. 24 shows a band pattern of the locus Mago19 showing that there was a specific CDO insertion in four dolphin superfamily (Miluaceae: Coccinella vulgaris, Band dolphin; Muricidae: Dall's porpoise; narwhal: narwhal). This locus strongly supports the monophyly of the dolphin superfamily.

FIG. 25 shows the results of alignment of the locus Mago19.

FIG. 26 shows the results of alignment of the locus Mago19.

FIG. 27: Loci Isi14, which indicates that CDO was inserted in 4 species of dolphin superfamily, 2 species of South American river dolphin, Amazon river dolphin, La Plata river dolphin, and White cormorant dolphin
The band patterns of Isi36 and Isi38 are shown.

FIG. 28: Locus Mago24, which shows that CDO was inserted in 4 species of dolphin superfamily, 2 species of South American river dolphin, Amazon river dolphin, La Plata river dolphin, and White cormorant dolphin.
The band patterns of Mago26 and Mago32 are shown.

FIG. 29 shows the results of the alignment of the Ishi14 locus.

FIG. 30 shows the results of alignment of the Ishi36 locus.

FIG. 31 shows the results of alignment of the Ishi36 locus.

FIG. 32 shows the results of the alignment of the Ishi38 locus.

FIG. 33 shows the results of the alignment of the locus Ishi38.

FIG. 34 shows the results of alignment of the locus Mago24.

FIG. 35 shows the results of alignment of the locus Mago24.

FIG. 36 shows the results of alignment of the locus Mago26.

FIG. 37 shows the results of alignment of the locus Mago26.

FIG. 38 shows the results of alignment of the locus Mago32.

FIG. 39 shows the results of alignment of the locus Mago32.

FIG. 40 shows band patterns of loci Mago8 and Mago13 indicating that CDO insertions specific to Delphinida and two whales belonging to the family Whale family, Great Gray Whale, were included.

FIG. 41 shows the results of alignment of the locus Mago8.

FIG. 42 shows the results of alignment of the locus Mago13.

FIG. 43 shows banding patterns of loci Mago21 and Mago22 indicating that CDO-specific insertions in Delphinida, red whale, and scorpionfish have been performed.

FIG. 44 shows the results of alignment of the Mago21 locus.

FIG. 45 shows the results of alignment of the locus Mago22.

FIG. 46 shows the results of alignment of the locus Mago22.

FIG. 47 shows the results of alignment of the locus Mago22.

[Fig. 48] A locus Sperm8, Sp indicating that CD insertion was common to all species of the subgenus Whale, including sperm whales.
The band patterns of erm28 and Sperm47 are shown.

FIG. 49 shows the results of alignment of the locus Sperm8.

FIG. 50 shows the results of the alignment of the locus Sperm8.

FIG. 51 shows the results of the alignment of the locus Sperm28.

FIG. 52 shows the results of the alignment of the locus Sperm47.

FIG. 53 shows band patterns of Amz13 and Bando1 loci showing that CDO was commonly inserted in two species of river dolphins in South America, Amazon river dolphin and Laplata river dolphin.

FIG. 54 shows the results of the alignment of the locus Amz13.

FIG. 55 shows the band pattern of Amz11 locus showing that CDO was specifically inserted in Amazon dolphins.

FIG. 56 shows the results of alignment of the locus Amz11.

FIG. 57 shows band patterns of Tuti24 and Tuti35 loci showing that CDO was specifically inserted in the Red Whale family.

FIG. 58 shows the results of alignment of the Tuti24 locus.

FIG. 59 shows the results of alignment of the Tuti35 locus.

FIG. 60 shows the results of alignment of the Tuti35 locus.

FIG. 61 shows the band patterns of the locus Sp9 indicating that CDO was inserted in three sperm whales and the sperm whale specific Sp2.

FIG. 62 shows the result of the alignment of the locus Sp2.

FIG. 63 shows the results of the alignment of the locus Sp2.

FIG. 64 shows the results of the alignment of the locus Sp9.

FIG. 65 shows band patterns of Hump20 and Hump203 loci showing that there was a specific CD insertion in the fin whale (or whiskers).

FIG. 66 shows the results of alignment of the locus Hump20.

Figure 67 shows the results of the alignment of the Hump20 locus.

FIG. 68 shows the results of alignment of the locus Hump20.

FIG. 69 shows the results of the alignment of the locus Hump203.

FIG. 70 shows a matrix summarizing SINE insertion patterns.

71 shows a phylogenetic tree in the whale order estimated based on the matrix shown in FIG. 70. FIG.

FIG. 72 shows the results (limits) of molecular statistical analysis.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 33/566 G06F 17/30 170F G06F 17/30 170 C12N 15/00 ZNAA F term (reference) 2G045 AA40 BA11 BB50 CB01 DA13 FB02 JA01 4B024 AA11 AA20 CA01 CA20 HA11 HA20 4B063 QA13 QA18 QA20 QQ02 QQ42 QQ50 QR08 QR32 QR40 QR42 QR62 QS16 QS25 QS31 QS40 QX01 QX10 5B075 UU19

Claims (35)

[Claims] 1. A method for discriminating animal species by the SINE method, comprising the steps of: preparing a genomic DNA library from each of one or more animal species to be discriminated; A clone containing an orthologous locus in which SINE belonging to a SINE family or subfamily is specifically inserted in at least one of the species is isolated; and located at both sides of the SINE family or subfamily at the locus. PC that anneals to flanking sequences
The sequences of the loci for each of the above animal species were amplified by PCR using a set of R primers; and the resulting PCR products were gel electrophoresed to yield the above SI
The species of the animal is discriminated by the presence or absence of a band indicating the presence of the NE family or subfamily insertion locus;
The method comprising: 2. The SINE family or subfamily is DNA having a sequence selected from the group consisting of SEQ ID NOS: 1 to 7 or SINE DNA hybridizing with the DNA under stringent conditions. the method of. 3. The method according to claim 2, wherein the SINE subfamily is a DNA having a CD sequence shown in SEQ ID NO: 6 or a CDO sequence shown in SEQ ID NO: 7. 4. A SINE family or subfamily D having a sequence selected from the group consisting of SEQ ID NOs: 3 to 7.
SINE DNA which hybridizes with NA or the above DNA under stringent conditions. 5. A Bando in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 8 to 21.
The method of claim 1, wherein the method is 1. 6. The Sp31, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 22 to 35.
The method of claim 1, wherein the method is 6. 7. The Mago in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 36 to 46.
The method of claim 1, wherein the method is 19. 8. The Ishi in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 47 to 51.
14. The method of claim 1, which is 14. 9. The Ishi in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 52 to 65.
The method of claim 1, wherein the method is 36. 10. The Ish in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 66 to 79.
The method of claim 1, wherein the method is i38. 11. The M orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 211 to 224.
The method of claim 1, which is ago24. 12. The M corresponding to the sequence shown in any one of SEQ ID NOs: 225 to 238, wherein the orthologous locus is M.
The method according to claim 1, which is ago26. 13. A mag, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 80 to 93.
The method of claim 1, wherein the method is o32. 14. A mag, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 94 to 98.
The method of claim 1, wherein the method is o8. 15. The Ma, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 99 to 112.
The method of claim 1, which is go13. 16. The M corresponding to the sequence shown in any one of SEQ ID NOs: 113 to 117, wherein the orthologous locus is M.
The method according to claim 1, which is ago21. 17. The M corresponding to the sequence shown in any one of SEQ ID NOs: 239 to 252, wherein the orthologous locus is M.
The method according to claim 1, which is ago22. 18. The S corresponding to the sequence shown in any one of SEQ ID NOS: 118 to 129, wherein the orthologous locus is S.
The method according to claim 1, which is perm8. 19. The S corresponding to the sequence shown in any one of SEQ ID NOs: 130 to 135, wherein the orthologous locus is S.
The method according to claim 1, which is perm28. 20. The S or the orthologous locus corresponding to the sequence shown in any one of SEQ ID NOs: 136 to 140.
The method according to claim 1, which is perm47. 21. The A, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 141 to 145.
The method of claim 1, wherein the method is mz13. 22. A in which the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 146 to 151.
The method of claim 1, wherein the method is mz11. 23. The T-corresponding to the sequence shown in any one of SEQ ID NOs: 152 to 156, wherein the orthologous locus is
The method of claim 1, wherein the method is uti24. 24. The T corresponding to the sequence shown in any one of SEQ ID NOs: 157 to 171 in which the orthologous locus is expressed.
The method of claim 1, wherein the method is uti35. 25. The orthologous locus corresponds to the sequence shown in any one of SEQ ID NOS: 172-185.
The method of claim 1, which is p2. 26. The S orthologous locus corresponding to the sequence shown in any one of SEQ ID NOs: 186 to 190.
The method of claim 1, which is p9. 27. The H, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 191-204.
The method of claim 1, which is ump20. 28. The H, wherein the orthologous locus corresponds to the sequence shown in any one of SEQ ID NOs: 205 to 210.
The method of claim 1, which is ump203. 29. A DNA having the sequence shown in any one of SEQ ID NOs: 8 to 238 or an orthologous DNA which hybridizes with the above DNA under stringent conditions. 30. The PCR primer is SEQ ID NO: 2.
The method of claim 1 selected from the group consisting of 76-325. 31. The animal of claim 1, wherein the animal is a mammal.
The method described in. 32. The method of claim 31, wherein the animal belongs to the Artiodactyla. 33. A method of obtaining a consensus sequence of the SINE subfamily, the steps of: sequencing multiple copies of a repeat unit by whole genome transcription in vitro or automatic sequencing of genomic DNA larger than 60 kbp; Aligning the sequences from the species to obtain a consensus sequence; RNA polymerase III
Find the consensus sequence of the second promoter for and make a stem-loop structure containing this sequence; 3'-PyPyPuPuPu-5 'sequence with 5 bases 5'upstream from the stem region as one unit Make sure that there is; t
The next 5 bases forming the anticodon-stem region of the RNA structure are considered as other units; the next 7 bases forming the anticodon-loop region of the tRNA structure are considered as still other units, where the 3 It was confirmed that the'end was an AA residue and the 5'end was a 3'-TC-5 'residue; the next 5 bases were regarded as another unit and assigned to the anticodon-stem region. Confirmation of base pair formation with 5 bases, where a deletion was placed at the position of the first base 3'to the anticodon-stem to align this unit correctly; Construct a stem-loop structure for the D region, where R above
2 in the first promoter region of NA polymerase III
The presence of two G's; the sequence similarity between the sequence and the tRNA was searched using a DNA database to confirm its secondary structure similarity; as described above from multiple species. The resulting SINE family sequences are aligned and the presence of characteristic nucleotides or deletions results in SIN
Obtaining the E subfamily; and obtaining the SINE subfamily consensus sequence from the SINE subfamily alignment. 34. The method according to claim 1, wherein the SINE subfamily is a DNA having a SINE subfamily consensus sequence obtained by the method for obtaining a SINE subfamily consensus sequence according to claim 33. 35. An S obtained by the method for obtaining a consensus sequence of the SINE subfamily according to claim 33.
DN with consensus sequence of INE subfamily
A.