JP2002300872A5 - Culture column for 3D cell culture device - Google Patents
Culture column for 3D cell culture device Download PDFInfo
- Publication number
- JP2002300872A5 JP2002300872A5 JP2001105102A JP2001105102A JP2002300872A5 JP 2002300872 A5 JP2002300872 A5 JP 2002300872A5 JP 2001105102 A JP2001105102 A JP 2001105102A JP 2001105102 A JP2001105102 A JP 2001105102A JP 2002300872 A5 JP2002300872 A5 JP 2002300872A5
- Authority
- JP
- Japan
- Prior art keywords
- culture
- column
- cell culture
- culture device
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 title description 5
- 239000002245 particle Substances 0.000 description 7
- QORWJWZARLRLPR-UHFFFAOYSA-H Tricalcium phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 4
- 239000001506 calcium phosphate Substances 0.000 description 4
- 229910000389 calcium phosphate Inorganic materials 0.000 description 3
- 235000011010 calcium phosphates Nutrition 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 1
- 238000001354 calcination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- FDFYYWMHPJTGEO-UHFFFAOYSA-K tetracalcium;phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O FDFYYWMHPJTGEO-UHFFFAOYSA-K 0.000 description 1
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 1
- 235000019731 tricalcium phosphate Nutrition 0.000 description 1
- 229940078499 tricalcium phosphate Drugs 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 緻密又は多孔質球状で粒径が平均粒径±１５％の範囲にある真球状のリン酸カルシウム系セラミックス粒子を培養容器に最密充填して形成される３次元細胞培養装置用培養カラム。
【請求項２】 湿式合成したハイドロキシアパタイトスラリーを仮焼して得られる原料粉末に1:３の割合でバインダーを加え、これを液体窒素に滴下して凍結粒子を得た後、凍結乾燥させ、焼成して得られる球状粒子を培養容器に最密充填する３次元細胞培養装置用培養カラムの製造方法。
【請求項３】 前記リン酸カルシウム系セラミックス粒子の粒径が５００μ±７５μｍである請求項１、２に記載の３次元細胞培養装置用培養カラム。
【請求項４】 前記リン酸カルシウム系セラミックスが、ハイドロキシアパタイト、リン酸三カルシウム、リン酸四カルシウムから選ばれる請求項１に記載の３次元細胞培養装置用培養カラム。
[Claims]
(1) A culture column for a three-dimensional cell culture device, which is formed by densely or densely spherical spherical calcium phosphate-based ceramic particles having a particle diameter in the range of an average particle diameter of ± 15% in a culture vessel.
(2) A binder is added at a ratio of 1: 3 to the raw material powder obtained by calcining the wet-synthesized hydroxyapatite slurry, which is dropped into liquid nitrogen to obtain frozen particles, which are then freeze-dried and fired. A method for producing a culture column for a three-dimensional cell culture device, wherein spherical particles are closely packed in a culture vessel.
(3) 3. The culture column for a three-dimensional cell culture device according to claim 1, wherein the calcium phosphate-based ceramic particles have a particle size of 500 μ ± 75 μm.
(4) The culture column for a three-dimensional cell culture device according to claim 1, wherein the calcium phosphate-based ceramic is selected from hydroxyapatite, tricalcium phosphate, and tetracalcium phosphate.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001105102A JP2002300872A (en) | 2001-04-03 | 2001-04-03 | Three-dimensional cell culture support and system using the same support |
| PCT/JP2002/003358 WO2002081620A1 (en) | 2001-04-03 | 2002-04-03 | Three-dimensional cell culture systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001105102A JP2002300872A (en) | 2001-04-03 | 2001-04-03 | Three-dimensional cell culture support and system using the same support |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002300872A JP2002300872A (en) | 2002-10-15 |
| JP2002300872A5 true JP2002300872A5 (en) | 2008-05-15 |
Family
ID=18957854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001105102A Pending JP2002300872A (en) | 2001-04-03 | 2001-04-03 | Three-dimensional cell culture support and system using the same support |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2002300872A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4631049B2 (en) * | 2005-03-18 | 2011-02-16 | 学校法人明治大学 | Bioreactor |
| JP5280432B2 (en) * | 2007-04-27 | 2013-09-04 | ヒュンジン ヤン | Cell culture support having increased specific gravity and method for producing the same |
| FR2979110B1 (en) * | 2011-08-16 | 2013-09-27 | Etat Francais Ministere De La Defense Service De Sante Des Armees | IN VITRO MODELING OF HEMATOPOIETIC STEM CELL MEDICINAL NICHES: A TOOL FOR STUDYING REGULATION OF HEMATOPOIESE, EVALUATING THE POTENTIAL OF NICHING A HEMATOPOIETIC GRAFT AND TESTING THE PHARMACO-TOXICOLOGY OF MEDICINAL PRODUCTS. |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0576364A (en) * | 1991-09-20 | 1993-03-30 | Kanegafuchi Chem Ind Co Ltd | Immobilized product of animal free cell, immobilizing method and culturing method |
| JP3635316B2 (en) * | 1996-11-25 | 2005-04-06 | 株式会社アドバンス | Method for producing spherical ceramic porous body |
| JP2001019570A (en) * | 1999-07-01 | 2001-01-23 | Advance Co Ltd | Apparatus for producing ceramics |
-
2001
- 2001-04-03 JP JP2001105102A patent/JP2002300872A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Martin et al. | Mechanical properties of hydroxyapatite formed at physiological temperature | |
| JP3362267B2 (en) | Bioimplant material and method for producing the same | |
| US6187046B1 (en) | Prosthetic bone material and process for the production of the same | |
| Murray et al. | An improvement in processing of hydroxyapatite ceramics | |
| US4889833A (en) | Granular inorganic moldings and a process for production thereof | |
| Zhu et al. | Composition–structure–property relationships of the CaO–M x O y–SiO 2–P 2 O 5 (M= Zr, Mg, Sr) mesoporous bioactive glass (MBG) scaffolds | |
| Kim et al. | In vitro biodegradable and mechanical performance of biphasic calcium phosphate porous scaffolds with unidirectional macro-pore structure | |
| Miculescu et al. | Influence of the modulated two-step synthesis of biogenic hydroxyapatite on biomimetic products’ surface | |
| JPS62295666A (en) | Continuous two-dimensional porous implant material and its production | |
| Ezhaveni et al. | Preparation and characterization of nano-hydroxyapatite nanomaterials for liver cancer cell treatment | |
| Swain et al. | Preparation of high strength macroporous hydroxyapatite scaffold | |
| AU2015281761B2 (en) | Method for producing porous calcium deficient hydroxyapatite granules | |
| Tangboriboon et al. | Alternative green preparation of mesoporous calcium hydroxyapatite by chemical reaction of eggshell and phosphoric acid | |
| JP2002300872A5 (en) | Culture column for 3D cell culture device | |
| CN102633497B (en) | The biphase ceramics of dicalcium phosphate pottery, dicalcium phosphate and oxyhydrogen-base apatite and manufacture method thereof | |
| JP2756703B2 (en) | Spherical apatite, method for producing the same, and molded article with porous structure | |
| JP2001198208A (en) | Calcium phosphate type biocompatible ceramic sintered body and method of manufacturing the same | |
| PL210026B1 (en) | Method of manufacturing the high-porous, bioactive calcium phosphate implant material | |
| JP2506826B2 (en) | Granular inorganic molded body and method for producing the same | |
| JPH067148A (en) | Carrier for culturing cell and method for culturing cell | |
| Lilley et al. | Brushite cements from polyphosphoric acid, calcium phosphate systems | |
| JPS61242968A (en) | Forming material | |
| Oh et al. | Synthesis and Properties of Bone Cement Containing Dense ß-TCP Granules | |
| Medvecky et al. | Calcium Phosphate Cement Modified with Silicon Nitride/Tricalcium Phosphate Microgranules | |
| Câmara-Pereira et al. | Characterization of Biomaterial and Tissue Response Analysis after Implantation |