JP2002257821A - Specimen immobilizing method and sample slide preparing device utilizing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To reliably form the metaphase of a specimen into an appropriate shape by controlling related parameters on a sample slide preparing method, to prepare appropriate sample slides for anybody, to provide the sample slides of stable quality, and to mass-produce them. SOLUTION: The liquid specimen is dried for drying time by which the metaphase of the specimen is formed into an appropriate shape.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a specimen fixing method for fixing a specimen to a specimen slide when producing a specimen slide of cells or chromosomes, and to a specimen slide preparing apparatus using the method. The present invention relates to a sample fixing method capable of forming a sample into an appropriate shape and a preparation apparatus using the method.

[0002]

2. Description of the Related Art In general,
The chromosome examination process is generally divided into five stages: cell culture, cell harvest and preparation of chromosome specimen slides, staining of chromosome bands by the staining method, micrograph photography, and karyotyping.
The present invention is mainly directed to cell harvesting and preparation of chromosome specimen slides.

[0003] In the second step, cell harvesting and preparation of chromosome specimen slides, the cells in the first step, which are metaphase cells in which colcemid is acted on, ie, metaphase, are subjected to hypotonic treatment, This is a process in which a cell membrane or a nuclear membrane is destroyed by applying a shock, and the chromosome that was present in the nucleus, which is a sphere, is developed on a slide glass, and a sample slide in which the metaphase is spread in an appropriate shape as a sample is created. There is a need to If the specimen slide is not finished to a beautiful one, all subsequent operations such as staining of the chromosome band become extremely difficult.

[0004] In the preparation of slides for chromosome specimens, various fixing methods of manually dropping a cell suspension containing chromosomes onto a slide glass have been practiced as a craftsmanship.

As a manual method, there is, for example, a method shown in FIGS. In the flame fixing method shown in FIG.
Drop the cell suspension 3 from the pipette 2 on the top 4 and spread it,
Thereafter, the specimen slide 1 is quickly passed through the flame from the burner 5 to ignite methanol, and the liquid suspension 3 is evaporated to prepare a specimen. In the vapor fixing method shown in FIG. 9, the specimen slide 1 on which the cell suspension 3 is dropped is brought close to the vapor 8 heated by the superheater 6 and evaporated from the container 7 in the same manner as in FIG. Then, the liquid suspension 3 is evaporated to prepare a specimen. In addition, in the fixing method under high temperature and high humidity shown in FIG. 10, a slide glass is placed in a high temperature and high humidity state generated from a wet paper towel 10 heated by a hot plate 9, and the slide glass is placed thereon similarly to FIG. The cell suspension 3 is dropped and spread, and the liquid suspension 3 is evaporated to prepare a specimen. In addition, in the method of fixing at room temperature by dropping from a height shown in FIG. 11, the cell suspension 3 is placed on the workbench 11 and from a height of several millimeters, sometimes about 1.5 meters. Is spread by dropping 4, and the sample of the cell suspension 3 is evaporated to prepare a sample.

[0006] However, in these methods,
Skill is required to form the metaphase of the chromosome of the cell suspension 3 into an appropriate shape, and further, it involves an accidental element to enable generation of the metaphase of the appropriate shape. It is difficult to make a simple specimen slide,
It was not an operation method that anyone could do.

[0007] Further, in these methods, related parameters for enabling generation of a metaphase having an appropriate shape have not been clarified, and the development procedure is based on the experience of a researcher or the like operating at present. Was.

[0008] In the preparation of the above-mentioned conventional specimen slides which are produced by the craftsman technique, it is very difficult for an inexperienced operator to prepare a specimen slide in which the metaphase is spread in a proper shape as a specimen. It was difficult. Furthermore,
These methods have poor reproducibility of manual conditions, and have a problem in the stability of the quality of specimen slides since the test is performed manually.

The present invention has been made in view of these points, and by controlling related parameters related to a method for preparing a specimen slide, a metaphase of a specimen can be surely formed into an appropriate shape, so that anyone can properly adjust the metaphase. It is an object of the present invention to provide a specimen fixing method capable of preparing a specimen slide with a stable quality and a mass production, and a specimen slide preparing apparatus using the method. .

[0010]

Means for Solving the Problems The present inventors have made intensive studies to achieve the above-mentioned object, and have found that a metaphase having a proper shape can be produced by drying a liquid specimen with a predetermined drying time. And completed the present invention.

[0011] Further, the present inventors, as the control parameters of the drying time, when the metaphase is developed by dropping a cell suspension as a specimen onto a sample slide, the temperature, humidity, The present inventors have found that it is desirable to control the amount of the sample dropping solution, the concentration of acetic acid in the Carnoy's solution of the cell suspension, and the like, and completed the present invention.

That is, the specimen fixing method of the present invention is a specimen fixing method for fixing a specimen consisting of cells or chromosomes to a specimen slide, wherein the liquid specimen is dried with a drying time at which the metaphase of the specimen has an appropriate shape. Features.

With this configuration, it is possible to obtain a specimen slide in which the metaphase of the specimen is fixed in an appropriate shape by drying the liquid specimen with a predetermined drying time.

Further, the method for fixing a specimen of the present invention is characterized in that the diameter of the metaphase of the specimen is from 60 to 100 μm and the drying time is from 100 to 300 seconds.
The metaphase of the specimen can be further fixed in a proper shape.

Further, the drying time may be controlled using the temperature and humidity of the sample drying atmosphere, the amount of the liquid sample dropped, the Carnoy's acetic acid concentration of the cell suspension, and the like as control parameters.

By properly controlling such control parameters, the metaphase of the specimen can be fixed to a more appropriate shape.

[0017] The specimen slide preparing apparatus of the present invention comprises:
In a sample slide preparing apparatus for preparing a sample slide by fixing a sample composed of cells or chromosomes to a sample slide, a drying control mechanism for drying a liquid sample with a drying time in which a metaphase of the sample on the sample slide has an appropriate shape. It is characterized by having.

With this configuration, it is possible to dry the liquid specimen for a predetermined drying time by the drying control mechanism and to prepare a specimen slide in which the metaphase of the specimen is fixed in an appropriate shape.

The drying control mechanism can be used to control and control the temperature and humidity of the drying atmosphere, the amount of liquid sample drop, the Carnoy's acetic acid concentration of the cell suspension, and the like. In addition, it is preferable that the metaphase of the specimen on the specimen slide obtained in advance is controlled according to a control loop that controls according to a drying time at which the metaphase has an appropriate shape.

With this configuration, the above-mentioned control parameters can be appropriately controlled, and as a result, the metaphase of the specimen can be fixed to a more appropriate shape.

Further, the sample slide preparing apparatus of the present invention further includes a liquid sample preparing mechanism for obtaining a liquid sample dropped on the sample slide, so that the processes from sample preparation to sample fixing can be performed well. Can be.

[0022]

FIG. 1 is a block diagram showing an embodiment of the present invention.
This will be described with reference to FIG.

FIG. 1 shows a sample slide preparation apparatus 1 according to the present invention.
It is a schematic diagram showing the basic composition of an embodiment.

The specimen slide preparing apparatus 20 of the present embodiment is provided with a closed space 21 including an appropriate chamber or the like for drying a specimen. A constant temperature block 23 having a built-in heater 22 for heating the cell suspension 3 serving as a specimen dropped on the upper surface of the specimen slide 1 made of a slide glass is installed in a lower portion of the closed space 21. Heater 22
A temperature sensor 24 is disposed in the vicinity of the closed space 2.
The heating is controlled by an outside constant temperature block temperature control unit 25. In the upper part of the closed space 21,
A humidifier 26 is provided. This humidifier 26 is a container 2
The water in 7 is heated by a humidifying heater 28 to generate steam. The generated steam is fan 2
9 causes forced convection in the closed space 21. Above the closed space 21, a humidity sensor 30 is provided.
Humidity is controlled by a humidifying heater control unit 31 outside the closed space 21. The cell suspension 3 is pre-cultured and then prepared by hypotonic treatment, and a predetermined amount is sent out through a transport pipe 34 by a supply pump 33 provided in a pump control unit 32 installed outside the closed space 21. The liquid is supplied dropwise to the upper surface of the specimen slide 1 through a drip pipette 35 installed in the closed space 21. Further, outside the closed space 21, there is provided an optimum deployment temperature value calculation unit 36 for controlling the operation of the constant temperature block temperature control unit 25. The optimal development temperature value calculation unit 36 receives the humidity value from the humidity sensor 30, the supply amount of the cell suspension 3 from the pump control unit 32, that is, the dropping amount value of the sample, and the Carnoy's solution given at the time of preparing the sample. The temperature setting value calculated based on the acetic acid concentration value is output and provided to the constant temperature block temperature control unit 25 through the manual / automatic control switch 37.

The fixing of a specimen and the preparation of a specimen slide by the specimen slide preparing apparatus 20 thus formed will be described.

First, the sample slide 1 is placed on the upper surface of the constant temperature block 23 in a heated state. Next, a predetermined amount of the cell suspension 3 is sent out by the supply pump 33 of the pump control unit 32, and the specimen slide 1 is passed through the dropping pipette 35.
Drop on top. At this time, the temperature in the closed space 21 is set to a predetermined value by the constant temperature block temperature control unit 25 energizing the heater 22 of the constant temperature block 23 in accordance with the temperature set value instructed by the optimum deployment temperature value calculation unit 36. Is controlled. At the same time, the humidity in the closed space 21 is controlled by the humidification heater control unit 31 to control the humidification heater 28 based on the humidification set value so as to become a set value.
The specimen on the specimen slide 1 is dried by the drying atmosphere in the closed space thus formed. In this device 20, the Carnoy's solution acetic acid concentration of the cell suspension is kept constant,
Since an accurate drop volume can be realized by using the precise supply pump 33, these two parameters can be fixed at constant values.

The present inventors have conducted intensive studies using human leukocytes as a specimen. As a result, the dried metaphase on the specimen slide 1 had a diameter of 60 to 100 μm in an appropriate shape. Furthermore, when the drying time of the sample in this case was set to 100 to 300 seconds, a metaphase having a proper shape of the sample was obtained. Here, the proper shape of the metaphase means a state in which all of the 46 chromosomes are within a predetermined diameter in a state of being completely separated without any duplication.

Therefore, in the specimen slide preparing apparatus 20 shown in FIG. 1, in order to realize a predetermined drying speed, a drying time at which the metaphase of the specimen on the specimen slide 1 has an appropriate shape is determined in advance, and this is determined. Combination data such as the temperature and humidity of the drying atmosphere in the closed space 21 when the drying time is realized, the amount of the liquid sample dropped, the Carnoy's solution acetic acid concentration of the cell suspension, and the like are determined, and these data are used as a control loop. The temperature and humidity of the dry atmosphere, the amount of the liquid sample drop, the Carnoy's solution acetic acid concentration of the cell suspension, etc. are stored in the memory within the optimal development temperature value calculation unit 36 based on the data when the next sample slide is prepared. The operation control is performed so as to reproduce.

By controlling the drying speed as a parameter related to the method of preparing the sample slide 1, the metaphase of the specimen can be surely formed in an appropriate shape, and an appropriate sample slide 1 can be prepared by anyone. And the quality of the specimen slide 1 is stabilized.

The drying speed as a related parameter will be further described.

The closed space 21 when the drying time is realized
The drying time is given by the following equation based on the temperature and humidity of the drying atmosphere in the inside, the amount of the liquid sample to be dropped, and the concentration of acetic acid in the Carnoy's solution of the cell suspension.

That is, assuming that drying time (tvap), temperature (T), humidity (RH), acetic acid concentration (D), and liquid volume (V), tvap = F (T.RH.DV) Can be.

The parameter sensitivity (change rate) can be obtained by calculating the change.

That is, Kt = ∂F / ∂T = ∂tvap / ∂T <O Krh = ∂F / ∂RH = ∂tvap / ∂RH> O Kd = ∂F / ∂D = ∂tvap / ∂D> O Kv = ∂F / ∂V = ∂tvap / ∂V> 0 Parameter sensitivity (rate of change) for these drying times
Has a tendency of temperature (-), humidity (+), acetic acid concentration (+), and liquid volume (+). The drying time (tvap) gives a function that can be regarded as a linear function in a range where the temperature (T), humidity (RH), acetic acid concentration (D), and drop volume (V) do not significantly change. That is, tvap = Kt · T + Krh · RH + Kd · D + Kv · V Here, when the drying time (tvap) is controlled to the best value under the developing condition in which the acetic acid concentration (D) and the liquid volume (V) are constant, It is understood that the humidity or the temperature may be controlled.

A control coefficient can be calculated from these change constants, and a temperature, a humidity, a dropping liquid amount, and a Carnoy's solution acetic acid concentration of a cell suspension are given as current values to provide an optimum drying time. Environment can be controlled. Although these parameters have an appropriate range, humidity is difficult to precisely control vapor diffusion, liquid volume control affects the number of developing cells in the sample, and Carnoyer's acetic acid concentration control has a narrow dynamic range of control. There is a problem that feedback control is difficult. Therefore, the temperature condition is a parameter that can be easily controlled.

FIGS. 2 and 3 show another embodiment of the sample slide preparing apparatus of the present invention. In addition to fixing the sample shown in FIG. 1, a liquid sample for dropping a liquid sample onto the sample slide 1 is obtained. It has a sample preparation mechanism.

The sample slide preparing device 40 of the present embodiment
A constant temperature block 23 for fixing a sample formed in the same manner as in FIG. 1 is disposed on the right front side of a flat rectangular base 41. The slide glass supply cassette section 42 adjacent to the left side The sample slides 1 are supplied one by one to the upper surface of the thermostatic block 23. A liquid sample preparation mechanism 43 for obtaining a liquid sample dropped on the sample slide 1 is provided behind the constant temperature block 23. The liquid sample preparation mechanism 43 mainly includes a centrifuge 44 and an XY liquid dropping liquid sample on the sample slide 1.
It is formed by a Z movable pipette mechanism 45.

One of the XYZ movable pipette mechanisms 45 is
Two elevated rails 46 are provided on both left and right sides of the constant-temperature block 23 and the centrifugal separator 44 in the front-rear direction, and a bridge 48 provided between both elevated rails 46 is provided with a roll 48 provided at both ends thereof. A pipette supporting and traveling body 50 is movably mounted on the elevated rail 46 with a roll 49 on the bridging rail 47, and a dropping pipette 35 is provided on the pipette supporting and traveling body 50 so as to be vertically movable. Have been.

The other centrifuge 44 will be described with reference to FIGS.

The centrifugal separator 44 is formed by fixing a rotating member 53 to the open end of a rotating shaft 52 of a centrifugal rotating mechanism 51 such as a motor. The moving buckets 54 are arranged at equal intervals on a circumference around the rotation shaft 52. The swing bucket 54 holds spits 55 so as to be rotatable around a central axis. Further, the centrifuge position detecting mechanism 5
6 is configured to detect that each spitz 55 has stopped at a predetermined stop position. Specifically, the sensor 57 detects a stop position mark (not shown) formed on the outer periphery of the sensor disk 57a fixed to the rotating shaft 52.

Around the centrifugal separator 44, a hypotonic solution pipette 58 for injecting a predetermined amount of a liquid reagent (hypotonic solution or Carnoy's fixative) into a spitz 55 held by the swinging bucket 54; A waste liquid pipette 59 for discharging a predetermined amount of liquid material from the spitz 55 is provided. At the position of the hypotonic solution pipette 58, a Carnoy's fixative solution pipette 60 is also arranged as shown in FIG. In addition, the lower part of the position of the hypotonic solution pipette 58 is provided with a gripper 61 which is extended upward from below by a stirring and lifting mechanism 62.
An agitating mechanism 63 having a driving means (not shown) for gripping the lower end of each spitz 55 stopped and moving it up and down, and rotating the shaft in the forward and reverse directions by the swinging bucket 54 is provided. The hypotonic solution in the hypotonic solution reagent bottle 64 is supplied to the hypotonic solution pipette 58 by the transport pump 65. Acetic acid bottle 6
The acetic acid in 6 and the methanol in the methanol bottle 67 are transported at a predetermined ratio (1: 3 in the present embodiment) by the transport pumps 68 and 69, respectively, and mixed in the two-liquid mixing unit 70 on the way. Will be sent. The waste liquid in the Spitz tube 55 is sucked from the discharge pipette 59 by a transport pump (not shown), and
It is discharged into 1. In the waste liquid portion, as shown in FIG. 4, the waste liquid level detector 72 is formed so that the amount of waste liquid in the Spitz tube 55 can be detected.

The centrifugal rotation mechanism 51, the centrifuge position detector 56, the stirring mechanism 63, and the transport pump 6
Reference numerals 5, 68, and 69 denote the specimen slide preparing device 4 respectively.
The control unit 73 controls the operation of each unit.

Next, the operation of the present embodiment will be described with reference to FIGS.

FIG. 5 is a flowchart showing processing in the first stage for preparing a sample slide glass by the sample slide preparing apparatus of the present invention. FIG. 6 is a flowchart showing processing in the second stage. FIG. 7 is a flowchart showing a process in the third stage.

First, as a preliminary step, the suspension of the cultured cells is transferred to six spitz 55 held in each swinging bucket 54 of the centrifuge 44.

Thereafter, the centrifuge 44 is driven to
Centrifuge at 300 rpm for 10 minutes, and stop one spitz 55 (hereinafter referred to as an operation on this one spitz 55) at the position of the waste pipette 59 by the centrifuge position detecting device 56 described above. (Step ST
1).

Then, the waste liquid pipette 59 is prevented from contacting the stopped spitz 55 with the inner wall of the spitz 55 from above the spitz 55 so that the pipette 59 for the waste liquid has a predetermined depth in the supernatant liquid layer in the spitz 55. The waste liquid is then inserted using the waste liquid level detector 72, and then a waste liquid pump (not shown) is driven to suck out the supernatant (about 5 ml) from the spitz 55 and collect it in the waste liquid tank 71 (step ST2).

When the extraction of the supernatant liquid is completed, the waste liquid pipette 59 is withdrawn from the spitz 55, and the centrifuge rotating mechanism 51 is driven to move the spitz 55 to the position of the hypotonic liquid pipette 58. And a predetermined amount of 5 ml of the hypotonic treatment liquid is injected by driving the transport pump 65 from the hypotonic liquid pipette 58 at the same position, and then the Spitz 55 is eccentrically stirred like a pestle. (Step ST3).

In this manner, the spitz 55 is sequentially moved in the rotation direction of the rotating member 53 by one stop position,
The processing of steps ST2 and ST3 is performed.

Thereafter, the rotating member 53 is stopped for about 15 minutes, and the temperature is maintained at 37 ° C. to perform a hypotonic treatment (step ST4).

Thereafter, the second stirring at the position of the hypotonic solution pipette 58 is sequentially performed on all the spits 55 (step ST5).

The Spitz 5 after the second stirring was completed.
5 is subjected to the same low tone processing as in step ST4 (step ST6).

Thereafter, at the position of the hypotonic solution pipette 58, the transport pump 6 is moved from the Carnoy's fixative solution pipette 60.
The first time, 0.5 ml of Carnoy's fixative is injected by driving 6, 67, and then Spitz 55 is eccentrically stirred like a pestle (step S).
T7).

In this way, the spitz 55 is sequentially moved by one stop position in the rotation direction of the rotating member 53,
The processing of step ST7 is performed.

Thereafter, the centrifuge 44 is spun down at 1300 rpm for 10 minutes (step ST8).

Next, as shown in FIG. 6, when the centrifugal separator 44 is stopped, about 5 ml of the spits 55 stopped at the position of the waste liquid pipette 59 in the same manner as in the step ST2. The supernatant liquid is sucked out of the spitz 55 and collected in the waste liquid tank 71 (step ST).
9).

When the second extraction of the supernatant is completed, the waste liquid pipette 59 is withdrawn from the spitz 55, and the centrifuge rotating mechanism 51 is driven to remove the spitz 55 for the hypotonic solution. Move to the position of the pipette 58, and at the same position, as in Step ST7,
From the Carnoa acetic acid liquid pipette 60, the transport pump 66,
The second predetermined amount of 3 m
l of Carnoy's fixative is injected, and then Spitz 55 is eccentrically stirred like a pestle (step ST1).
0).

In this way, the spitz 55 is sequentially moved by one stop position in the rotation direction of the rotation member 53,
The processing of steps ST9 and ST10 is performed.

When all the spits 55 have been stirred, the centrifugal separator 44 is driven at 1300 rpm for 6 minutes to spin down (step ST11).

At the time when the centrifuge 44 is stopped, the steps 55 are stopped at the position of the waste pipette 59 and the steps ST9 to ST1 are sequentially performed.
1 is repeated three to four times (step ST1).
2).

When the last centrifugation is completed, the process proceeds to the processing in FIG.

In steps ST13 and ST14 of FIG. 7, the above-described steps ST9 and ST1 are sequentially started from the spitz 55 stopped at the position of the waste liquid pipette 59.
The same processing as in step 0 is performed for the suction of the supernatant, the injection of the Carnoy's fixative, and the eccentric stirring of the spitz 55 to all spitz 55.

Thereafter, the transport pumps 66 and 67 are driven from the Carnoia acetic acid liquid pipette 60 in the same manner as in step ST14 for the spitz 55 stopped at the position of the hypotonic liquid pipette 58. The last Carnoy's fixative for adjusting the cell suspension concentration is injected, and then the Spitz 55 is eccentrically stirred like a pestle (step ST15). This process is performed for all Spitz 55
Apply to

Thereafter, the XYZ movable pipette mechanism 45 is operated to stop the dropping pipette 35, and the Spitz 5
Then, the cell suspension 3 in the spitz 55 is drawn into the dropping pipette 35, and is repeatedly discharged and subjected to a tapping process (step ST16).

Thereafter, the supply pump 33 is driven to collect the cell suspension 3 into the dropping pipette 35 (step ST17). Then, the XYZ movable pipette mechanism 45 is driven to pull out the dropping pipette 35 from the spitz 55, and then the XYZ movable pipette mechanism 45 is driven to move the dropping pipette 35 to above the spot position of the sample slide 1. , Then the XYZ movable pipette mechanism 4
5 is driven again to move the tip of the dropping pipette 35 to just above the upper surface of the specimen slide 1. Then, by driving the supply pump 33, the required several drops of the cell suspension 3 are dropped on the slide glass 19 from the dropping pipette 35 (step ST18).

Thereafter, the liquid cell suspension 3 on the specimen slide 1 is satisfactorily dried by the method described in the embodiment shown in FIG. 1 by the constant temperature block 23, and a metaphase having an appropriate shape is formed.

As described above, according to the present embodiment, it is possible to obtain the cell suspension 3 which is a liquid sample dropped on the sample slide 1 and to fix the liquid sample well.

The present invention is not limited to the above-described embodiment, but can be variously modified as needed.

[0069]

As described above, the specimen fixing method of the present invention and the specimen slide preparing apparatus using this method are constructed and operated, so that the related parameters relating to the specimen slide producing method are controlled. In addition, the metaphase of the specimen can be surely formed in an appropriate shape, anyone can make an appropriate specimen slide, and the quality of the specimen slide is stable, and mass production is possible. Has the effect of

[Brief description of the drawings]

FIG. 1 is an overall conceptual diagram showing an embodiment of a sample slide preparation device according to the present invention.

FIG. 2 is a front view showing another embodiment of the sample slide preparing device according to the present invention.

FIG. 3 is a plan view of FIG. 2;

FIG. 4 is a schematic diagram showing the configuration of a centrifuge according to the present invention.

FIG. 5 is a flowchart showing a process for preparing a sample slide glass using the sample slide preparing device according to the present invention.

FIG. 6 is a flowchart showing processing after FIG. 5;

FIG. 7 is a flowchart showing processing after FIG. 6;

FIG. 8 is a schematic diagram showing a conventional method for fixing a cell suspension.

FIG. 9 is a schematic view showing another conventional method for fixing a cell suspension.

FIG. 10 is a schematic view showing still another conventional method for fixing a cell suspension.

FIG. 11 is a schematic view showing still another method for fixing a conventional cell suspension.

[Description of Signs] 20, 40 Specimen slide preparation device 21 Closed space 23 Constant temperature block 25 Constant temperature block temperature control unit 26 Humidifier 31 Humidification heater control unit 32 Pump control unit 36 Optimal development temperature value calculation unit 43 Liquid sample preparation mechanism 44 Centrifuge 45 XYZ movable pipette mechanism 58 Pipette for hypotonic solution 60 Pipette for Carnoy's fixative

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Tomoko Nagaoka 2-7-7 Honcho, Funabashi-shi, Chiba F-term in Ad Science Co., Ltd. 2G045 BA14 BB21 BB23 BB49 BB50 CA01 CB01 CB30 FA16 HA16 JA07 2G052 AA33 DA07 FA05 HC22 HC23 HC24 2H052 AE07

Claims (8)

[The claims] 1. A specimen fixing method for fixing a specimen comprising cells or chromosomes to a specimen slide, wherein the liquid specimen is dried with a drying time during which a metaphase of the specimen becomes an appropriate shape. 2. The proper shape of the metaphase of the specimen is
The specimen fixing method according to claim 1, wherein the diameter is set to 60 to 100 µm. 3. The specimen fixing method according to claim 1, wherein the drying time is 100 to 300 seconds. 4. The method according to claim 1, wherein the drying time is controlled by controlling the temperature and humidity of a drying atmosphere, the amount of a liquid sample to be dropped, the concentration of Carnoy acetic acid in a cell suspension, and the like as control parameters. Item 4. The specimen fixing method according to any one of Items 3. 5. A specimen slide preparing apparatus for preparing a specimen slide by fixing a specimen comprising cells or chromosomes to a specimen slide, wherein the liquid specimen is dried with a drying time at which the metaphase of the specimen on the specimen slide has an appropriate shape. A specimen slide preparation device, comprising a drying control mechanism for causing the sample slide to be produced. 6. The drying control mechanism according to claim 1, wherein the temperature and humidity of the drying atmosphere, the amount of the liquid drop of the liquid sample, the concentration of the Carnoy's acetic acid in the cell suspension, and the like can be controlled. Item 6. The specimen slide preparation device according to Item 5. 7. The drying control mechanism includes a temperature and a humidity of a drying atmosphere, a drop amount of a liquid sample, and a cell suspension according to a drying time at which a metaphase of the sample on a sample slide obtained in advance has an appropriate shape. The specimen slide preparing apparatus according to claim 6, further comprising a control loop for controlling a Carnoy's acetic acid concentration of the liquid and the like. 8. The specimen slide producing apparatus according to claim 5, further comprising a liquid specimen producing mechanism for obtaining a liquid specimen dropped on the specimen slide.