JP2002249436A - Antibacterial agent using rush, manufacturing method thereof, and antibacterial material - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve problems associated with the limited amount of raw materials from which antibacterial agents can be obtained, and to provide an inexpensive and constant mass-production method of natural antibacterial agents, which can be used not only in food industry but also widely in other industrial fields, using an antibacterial substance derived from rush. SOLUTION: Dried rush is ground to powder and taken as an oral medication in tablet form or in powder. Alternatively, a liquid antibacterial agent is provided using extracts of rush ingredients. Since the antibacterial agent prevents the growth of and destroys harmful microorganisms, the agent can be used in food industry as a bacteriostat or a bactericide. When used as a food additive to prevent food spoilage, the agent is safer than chemical agents. The agent is safe when taken by a sitotoxism patient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to the use of a component of rush as an antibacterial agent (including a material) which can be used as a means for preventing harmful microbial contamination causing food poisoning such as Escherichia coli and Salmonella. The present invention relates to a method for producing a liquid antibacterial agent by extracting components.

[0002]

2. Description of the Related Art Conventionally, as means for preventing harmful microbial contamination in foods and foodstuffs, there are, for example, keeping at low temperature such as refrigeration and freezing, reducing water activity such as drying, controlling gas phase such as vacuum and oxygen scavenger, and salt. And an activity inhibitor such as an organic acid or an antibacterial agent (food additive). Among these, antibacterial agents have come to be used relatively frequently from the viewpoint of simplicity and convenience of measures for preventing harmful microorganism contamination.

[0003] Antibacterial agents are roughly divided into chemical agents and biological agents. Often used as chemical agents,
For example, sodium hypochlorite, hypochlorous acid, and the like.
Chemical agents are excellent in that they are inexpensive and provide a strong antimicrobial action even in a small amount, but have the problem of being highly toxic to the human body.

On the other hand, biological agents include microorganism-derived antibacterial agents generally called "antibiotic agents" and antibacterial agents extracted from some animals and plants. These are chemical agents. The antibacterial effect is inferior, but the safety is excellent. Accordingly, in recent years, many institutions have been conducting research on various naturally occurring antibacterial agents, and are working to discover natural antibacterial agents.

[0005]

The use of a natural antibacterial agent is, for example, sprayed for bacteriostasis or sterilization in many factories including the food industry, or used for preventing food spoilage. It can be used as an additive,
It can be taken when harmful microorganisms enter the body.It can be used in a wide range and is expected to be consumed in large quantities.However, raw materials that can be used with antibacterial agents are limited, so they are inexpensive. However, there is a problem that stable mass production is difficult.

[0006] Therefore, there is a problem which must be solved in order to stably supply raw materials which can be taken by the natural antibacterial agent, and to enable mass production at low cost.

The present inventors have conducted various studies focusing on the initial fungicidal properties of rush grass when rush grass is folded,
The present inventors have discovered that the components of rush can be used as antibacterial agents and completed the present invention.

[0008]

As a specific means for solving the above-mentioned problems, the present invention provides a method for pulverizing dried rush into fine powder to obtain a powdered or tableted swallow, In addition to providing an antibacterial agent using rush, which extracts components to form a liquid antibacterial agent, crushes or crushes rush and extracts the antibacterial substance of rush by immersing in an aqueous solution. Providing a method for producing an antibacterial agent, which comprises removing the crushed or crushed rush and producing a rush extract as a liquid antibacterial agent, and further crushing the rush to produce a fibrous portion. Is formed in the form of paper or nonwoven fabric, or the long fiber portion is a processed product of woven or knitted fabric.

In the method for producing an antibacterial agent according to the present invention, an additional requirement that the aqueous solution is water is included.

In the present invention, the antibacterial substance of rush is used as an antibacterial agent, and rush can be mass-produced and stably supplied as a raw material of the antibacterial agent while maintaining a predetermined quality. You can do it.

[0011]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Next, preferred embodiments of the present invention will be described in detail. The cut grass is used in a dried state, and the dried grass is crushed or crushed. In this case, the degree of crushing or pulverization of the rush differs depending on the manner in which the rush is subsequently used as an antibacterial agent, but can be appropriately selected from coarse to fine powder.

First, a description will be given of an example in which the production of a liquid antibacterial agent was conducted on a trial basis.
Then, 1 ml of water was added to the mixture, and the whole was stirred well, and then extracted at room temperature for 24 hours. After the extraction, the powdered rush is removed by filtering through a filter in which a plurality of gauze layers are piled up to obtain a rush extract, and the rush extract is used as a sample for measuring antibacterial activity (liquid antibacterial agent). However, antibacterial activity was obtained as described below. In addition,
The pH of the rush extract is around 6, which is completely different from the antibacterial action found in the high or low pH range.

[Measurement of antibacterial activity of rush extract] The antibacterial activity was measured by a paper disk method. After inoculating the test bacteria into a normal nutrient agar medium (liquid) manufactured by Eiken Chemical Co., Ltd. at 50 ° C, the mixture is solidified on a petri dish, and a paper disc (8 mm thick) manufactured by ADVANTEC is placed on the solidified medium. Then, 20 μl of a liquid antibacterial agent comprising the diluted rush extract was charged, and the petri dish was covered. The petri dish was cultured at 30 ° C. for 15 hours, and the presence or absence of an inhibition circle appeared around the paper disk was confirmed. The unit of activity was shown as dilution ratio × 1,000 / 20 (U / ml).

As test bacteria, Bacillus subtilis (Bac
hillus subtilis) IFO3335, Salmonella typhimurium IFO13245,
Micrococcus luteus IFO
3333, Escherichia coli IFO
3972, Staphylcoco
ccus aureus) IFO12732. The test bacterium was in a normal nutrient agar slant manufactured by Eiken Chemical Co., Ltd. at 30 ° C.
Was used for one week.

[0015] As a result, the liquid antibacterial agent comprising the rush extract showed activity values as shown in the following table, and antibacterial activity against each bacterium was observed. [Table] Bacterial name Antibacterial activity (U / ml) Bacillus subtilis 3200 U / ml Salmonella typhimurium 1600 U / ml Micrococca luteus 3200 U / ml Escherichia coli 6400 U / ml Staphylococcus aureus 100 U / ml

When the rush is used as an antibacterial agent, the rush can be finely powdered to be used as a powder, or it can be tabletted and used as a swallow (drug). In short, it can be naturally predicted that when taken as a swallow, the antibacterial substance of rush is extracted in the body and exerts an antibacterial action equivalent to that of the aforementioned rush extract.

Utilizing the grass having antibacterial properties as described above, a liquid antibacterial agent composed of a grass extract extracts bacteriostasis or sterilization in a food processing factory, a restaurant, a cafeteria, or a school lunch kitchen. For the purpose, it can be used not only as a safe spraying agent but also as a food additive for preventing food spoilage, and all of them can contribute to preventing food poisoning before it occurs. For food poisoning patients, fine powder of rush can be used as a powder or tablet.

Although only five kinds of microorganisms were selected as test bacteria in the previous experiment, they exhibited remarkable antibacterial activity against food poisoning bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus, etc. The above experiments suggest that antibacterial substances of rush can be used as antibacterial agents for a wide range of bacteria, regardless of whether they are gram negative or gram positive.

Furthermore, an antibacterial material can be obtained by utilizing the antibacterial properties of rush. That is, rush is crushed to convert the fiber into, for example, paper or non-woven fabric, or a part of the fiber is mixed into paper or non-woven fabric material, or finely split to form the long fiber original shape. It can be applied to processed products such as woven or knitted fabrics in the state of being left, and the processed products can be used as a packaging material for articles requiring antibacterial use, or for example, disposable diapers.

In any case, in the present invention, an antibacterial agent is obtained from rush, and rush can be mass-produced if cultivated by a rush farmer. It can be stably supplied in large quantities while keeping it, and furthermore, it is only necessary to crush or pulverize dried rush and use it as it is, or just immerse it in an aqueous solution to extract antibacterial substances, and the work process is extremely It is a simple, highly productive, and stable supply of safe antimicrobial agents.

[0021]

As described above, the antibacterial agent using rush of the present invention according to the present invention is obtained by pulverizing dried rush into fine powder to obtain a powdered or tableted swallow, or It extracts a component of grass to make a liquid antibacterial agent, and since the antibacterial agent has an action of preventing the growth of harmful microorganisms and killing it, it is intended for bacteriostatic or sterilization in the food industry. It can be used, and it is safer than chemical agents when used as a food additive to prevent food spoilage, and it is safe even if taken by patients with food poisoning This has the effect.

Further, the method for producing an antibacterial agent according to the present invention comprises:
Crushed or crushed rush, immersed in an aqueous solution to extract the antibacterial substance of rush, and then remove the crushed or crushed rush to produce a rush extract as a liquid antibacterial agent. The production process is remarkably simple, and rush can be produced in large quantities. Therefore, it can be supplied stably as a raw material of the antibacterial agent with a predetermined quality. Since it can be provided, it has an excellent effect that it can be provided relatively inexpensively.

Further, the antibacterial material according to the present invention is a material in which rush is crushed to form a fibrous portion in the form of paper or nonwoven fabric, or a long fiber portion is processed into a woven or knitted product, Because the processed product itself has an antibacterial effect,
It has an excellent effect that it can be applied to various wide applications requiring bacteriostasis or sterilization such as packaging materials.

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Claims (4)

[Claims] Claims 1. A dried rush grass is pulverized to a fine powder to obtain a powdered or tableted swallow, or a component of rush grass is extracted to obtain a liquid antibacterial agent. An antibacterial agent using grass. 2. The rush is crushed or crushed and immersed in an aqueous solution to extract the antibacterial substance of rush, and then the crushed or crushed rush is removed and the rush extract is used as a liquid antibacterial agent. A method for producing an antibacterial agent, which is produced. 3. The method for producing an antibacterial agent according to claim 2, wherein the aqueous solution is water. 4. An antibacterial material characterized by crushing rush grass to form a fibrous portion in the form of paper or nonwoven fabric, or forming a long fiber portion into a woven or knitted product.